CN113142073B - Multifunctional integrated cage for non-human primate experimental animals and use method thereof - Google Patents
Multifunctional integrated cage for non-human primate experimental animals and use method thereof Download PDFInfo
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- CN113142073B CN113142073B CN202110526594.XA CN202110526594A CN113142073B CN 113142073 B CN113142073 B CN 113142073B CN 202110526594 A CN202110526594 A CN 202110526594A CN 113142073 B CN113142073 B CN 113142073B
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- plate
- rack
- cage
- holding rod
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0005—Stable partitions
- A01K1/0017—Gates, doors
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
- A01K1/0356—Feeding or drinking devices associated with cages
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/027—Exercising equipment, e.g. tread mills, carousels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a multifunctional integrated cage for a non-human primate experimental animal and a use method thereof, wherein the multifunctional integrated cage comprises a cage body, the left side surface of the cage body is provided with an animal access gate, the front side surface of the cage body is a transparent plate, the rear side surface of the cage body is a background plate, and the top surface of the cage body is provided with a transverse holding rod area, a longitudinal holding rod area and an oblique holding rod area; the transverse holding rod area consists of a plurality of holding rods which are transversely distributed; the longitudinal holding rod area consists of a plurality of holding rods which are longitudinally distributed; the oblique holding rod area consists of a plurality of holding rods which are distributed obliquely; the top surface is also detachably provided with hanging rack components; the left side surface and the right side surface of the cage body are respectively provided with a plurality of feeding ports; an adjustable hanger component is also distributed on the outer side of the feeding port. The integrated cage can provide multiple behavioural testing functions, video recording, feeding monitoring and other functions, and has accurate, scientific and reliable data acquisition and good use effect.
Description
Technical Field
The invention relates to the technical field of multifunctional cages for non-human primate experimental animals, in particular to a multifunctional integrated cage for non-human primate experimental animals and a use method thereof.
Background
After relevant modeling, a non-human primate experimental animal (hereinafter referred to as experimental animal) generally needs to record or record relevant indexes, such as behaviours, for example, a model monkey with diabetes or senile dementia, and during the experimental period, the relevant limb fine activity indexes of the experimental animal need to be evaluated so as to judge the recovery condition after the relevant medicines are used. For example, the smoothness of the movements of the joints of limbs, fingers and the like, because the experimental animal cannot directly communicate with human beings, the objective evaluation currently has technical difficulties, especially the performance movement capability/level of the experimental animal which is naturally reflected is obtained under the influence of any external person, so that the objective and accurate evaluation is realized, and the technical problems which are needed to be solved are more urgent at present. The evaluation of the behaviours generally relates to video photography, and automatic analysis is generally performed through software at present after video photography, but the current related video acquisition cage has great defects, mainly that the current software is difficult to effectively distinguish part of animal limb actions in video, so that the related video cannot realize better automatic analysis. Meanwhile, in addition to the evaluation of the behaviours, the diabetic monkey needs to record the indexes such as water intake, urination and the like, because the related data are also found to be best acquired in the environment familiar to the experimental animal, after all, the state (such as impatience and the like) of the experimental animal in a new environment can be abnormal, and the acquisition of objective data is not facilitated, therefore, the multifunctional cage with functions of raising, behavioural testing and video recording is very necessary to integrate.
Disclosure of Invention
The invention provides a multifunctional integrated cage for a non-human primate experimental animal, which can provide a plurality of functions such as a behavioural test function and video recording and has good use effect, and a use method thereof.
In order to achieve the above object, the present invention adopts the following technical scheme:
the multifunctional integrated cage for the non-human primate experimental animals comprises a cage body, wherein an animal access gate is arranged on the left side surface of the cage body, the front side surface of the cage body is a transparent plate, the rear side surface of the cage body is a background plate, and the top surface of the cage body is provided with a transverse holding rod area, a longitudinal holding rod area and an oblique holding rod area; the transverse holding rod area consists of a plurality of holding rods which are transversely distributed; the longitudinal holding rod area consists of a plurality of holding rods which are longitudinally distributed; the oblique holding rod area consists of a plurality of holding rods which are distributed obliquely; the top surface is also detachably provided with hanging rack components; the left side surface and the right side surface of the cage body are respectively provided with a plurality of feeding ports; an adjustable hanger component is also distributed on the outer side of the feeding port;
the rack assembly comprises two rack fixing seats and a rack; a plurality of fixing holes are formed in the bottom plate of the rack fixing seat, and a fixing slot is formed in the middle of the bottom plate; the article hanging rack is 7-shaped as a whole, and the inclined edge of the article hanging rack is sequentially provided with a first layer inclined plate, a horizontal layer plate and a second layer inclined plate from bottom to top; the first layer inclined plate and the horizontal layer plate and the second layer inclined plate are respectively provided with a plurality of food insertion holes; the two ends of the object hanging rack are correspondingly inserted into the object hanging rack fixing seat to realize detachable fixing; the two hanging rack fixing seats are respectively fixed on the left side and the right side of the top surface of the cage body;
the adjustable hanging rack assembly comprises a hanging rack fixing seat and a hanging rack; the middle part of the hanging rack fixing seat is provided with a longitudinal slot; fixing holes are formed in two sides of the longitudinal slot; the object hanging rack comprises a 7-shaped socket and a 7-shaped adjusting rack; the sockets are detachably distributed in the longitudinal slots; a locking bolt is further arranged on the upper side of the socket; an adjusting long groove is formed on the inner side plate surface of the adjusting storage rack corresponding to the locking bolt; the adjusting long grooves are inserted into the locking bolts to be movably distributed and are pressed and fixed by the locking bolts; the outer side plate surface of the adjusting storage rack is provided with a plurality of food jacks along the inner and outer directions.
Furthermore, the upper plate surface of the socket is also provided with scales, and the scales are distributed corresponding to the adjusting long grooves.
Furthermore, the background plate is a milky white background plate, and an LED lamp strip is arranged in the background plate.
Further, the width and height dimensions of the background plate are larger than those of the front side surface.
Furthermore, the animal access gate is correspondingly provided with a memory test board fixing slot.
The method for detecting the behavioural exercise capacity of the multifunctional integrated cage for the non-human primate experimental animal comprises the following steps of:
(1) Feeding the experimental animals to be detected in the cage body in advance for one meal;
(2) Setting up a video recorder, wherein the maximum image pickup picture is in the range of the background plate;
(3) The adjustable shelf is adjusted to have a telescopic length relative to the socket according to the requirement, the locking bolt is tightened for fixation, and then the long food is fully inserted into the food jacks on the hanging shelf and the adjustable shelf respectively;
(4) After video recording is started, the object hanging frame and the object hanging frame are respectively inserted into the object hanging frame fixing seat and the object hanging frame fixing seat correspondingly, and then a technician immediately leaves the periphery of the cage body;
(5) The animal to be tested naturally shows the actions of limb coordination, climbing, arm pull-up and grabbing under the attraction of the long-strip food, so that the real movement capability of joints and fingers of the animal to be tested is known.
Compared with the prior art, the invention has the beneficial effects that:
(1) According to the invention, the milky white background plate with the LEDs is arranged, so that the contrast ratio between the limbs of the experimental animal and the background plate can be enhanced in the video monitoring process, and the distinguishing effect of the limb actions of the experimental animal and the obtaining effect of the definition are obviously improved.
(2) The invention is characterized in that a plurality of feeding ports are respectively arranged on the left side surface and the right side surface of the cage body; the adjustable hanging rack components are distributed on the outer side of the feeding port correspondingly, the telescopic length of the adjustable hanging rack components can be adjusted according to requirements, and when the experimental animals are monitored from the side to obtain foods with different heights and different distances, the natural and real upper limb activities and the finger grabbing fine action conditions without human factor interference are facilitated, objective comparison of the disease modeling process, the disease modeling process and the drug treatment process is facilitated, and objective evaluation is facilitated.
(3) According to the invention, the transverse holding rod area, the longitudinal holding rod area and the oblique holding rod area are arranged, and the object hanging rack assembly is matched, and meanwhile, the object hanging rack is correspondingly provided with a first inclined plate layer, a horizontal layer plate layer and a second inclined plate layer in sequence; the food on the three fetching layers can realize the attraction of three gradient foods, so that the experimental animal is prompted to hold the holding rod area by itself to be in a hanging state, and the limb movement condition of the experimental animal can be estimated; then attracting the food corresponding to the first inclined plate which is gradually inclined upwards to be grabbed in the transverse holding rod area, and acquiring the coordination capacity condition of limbs and the grabbing capacity evaluation of the food which is gradually becoming higher; the horizontal grip region is converted into the longitudinal grip region, and the oblique grip region is converted, so that the conversion grabbing capacity of the experimental animal at 90 degrees and other angles and the evaluation of joint movement capacity can be directly obtained; and the higher second-layer inclined plate food can shoot the action that the longer limb stretches out of the cage to grasp, gradually improves the grasping difficulty, is a test, can intuitively acquire relevant behavioral activity indexes, and realizes more intuitive, objective and accurate acquisition of required behavioural data.
Drawings
FIG. 1 is a schematic view of the cage of the present invention;
FIG. 2 is a schematic view of the structure of the adjustable hanger assembly of the present invention;
FIG. 3 is a schematic view of the structure of the hanger of the present invention;
FIG. 4 is a schematic view of a holder of the present invention;
fig. 5 is a schematic diagram of the complete combination of the present invention.
Description of the embodiments
As shown in fig. 1 to 5, a multifunctional integrated cage for a non-human primate experimental animal comprises a cage body, an animal access gate is arranged on the left side surface 3 of the cage body, a memory test board fixing slot 4 is correspondingly arranged at the animal access gate in this embodiment, that is, a dragging iron door with a rectangular structure is arranged at ordinary times for inserting and fixing after transferring the animal into the cage body, and when the behavioural memory capability test is required, a related test board which is commonly used at present is correspondingly inserted into the memory test board fixing slot 4, and the dragging iron door is pulled out for testing. The front side 1 of the cage body is a transparent plate, and can be supported by glass or acrylic materials, the rear side 9 is a background plate, the background plate of the embodiment is a milky background plate, an LED lamp strip is further arranged in the background plate and used for providing soft light, the contrast ratio is improved in video, relevant fine action details are better presented, the width and the height of the background plate of the embodiment are larger than those of the front side, the experimental animal moving range is in the background plate in video, the automatic analysis of follow-up software is facilitated, and the use effect is good. The top surface of the cage body is provided with a transverse grip region 6, a longitudinal grip region 7 and an oblique grip region 8; the transverse holding rod area 6 consists of a plurality of holding rods which are transversely distributed; the longitudinal holding rod area 7 consists of a plurality of holding rods which are longitudinally distributed; the oblique holding rod region 8 consists of a plurality of holding rods which are distributed obliquely, and is provided with the three holding rod regions, so that the three holding rod regions are mainly used for testing the conversion of the holding rod direction of an experimental animal in the climbing and holding rod hanging process, and visually evaluating the joint movement capability and the like of the experimental animal; the top surface is also detachably provided with hanging rack components; a plurality of feeding ports 5 are also respectively arranged on the left side surface and the right side surface of the cage body; an adjustable hanger component is also distributed on the outer side of the feeding port 5.
The rack assembly of the present embodiment includes two rack holders 26 and a rack; a plurality of fixing holes 28 are formed in the bottom plate of the rack fixing seat 26, and a fixing slot 27 is formed in the middle of the rack fixing seat; the whole article hanging rack is 7-shaped, and the inclined edge of the article hanging rack is sequentially provided with a first layer inclined plate 19, a horizontal layer plate 22 and a second layer inclined plate 23 from bottom to top; the first layer inclined plate 19, the horizontal layer plate 22 and the second layer inclined plate 23 are respectively provided with a plurality of food insertion holes 21; the two ends 20 and 25 of the rack are correspondingly inserted into the rack fixing seat to realize detachable fixation, in this embodiment, the stability of rack fixation is better realized, the two ends can be made of silica gel or rubber, friction force is improved, and the phenomenon of shaking and the like in the use process, especially in the animal feeding process, is prevented; the two rack holders 26 are respectively fixed on the left and right sides of the top surface of the cage body.
The adjustable hanger assembly of the embodiment comprises a hanger fixing seat 10 and a hanger; the middle part of the hanging rack fixing seat 10 is provided with a longitudinal slot 12; the two sides of the longitudinal slot 12 are provided with fixing holes 11, and when in use, the fixing holes 11 are penetrated by U-shaped bolts or iron wires to be fixed on the cage body; the object hanging frame comprises a 7-shaped socket 13 and a 7-shaped adjusting object placing frame 14; the sockets 13 are detachably distributed in the longitudinal slots 12; the upper side of the socket 13 is also provided with a locking bolt 16; an adjusting long groove 15 is formed on the inner side plate surface of the adjusting storage rack 14 corresponding to the locking bolt; the adjusting long slots 15 are inserted into the locking bolts 16 to be movably distributed and are pressed and fixed by the locking bolts 16, namely, the adjusting rack 14 is adjusted in a telescopic manner according to the age of the experimental animal as required so as to change the relative distance between food and the experimental animal, which is also used for detecting the limb coordination ability of the experimental animal; the outer side plate surface of the adjusting storage rack 14 is provided with a food fixing lug 17, a plurality of food inserting holes 18 are formed in the inner side and the outer side of the food fixing lug 17, and when the adjusting storage rack is used, strip-shaped food such as sweet potato strips, hawthorn strips, apple strips and the like can be inserted into the food inserting holes 18. Of course, in order to improve accuracy, a scale may be further disposed on the upper plate surface of the socket 13, where the scale corresponds to the distribution of the adjustment elongated slots.
Of course, for example, to the diabetes monkey can also be with dedicated accurate collection device of urine volume, have accurate measurement non-human primate experimental animal water intake function's watering device etc. integrated to on the cage body as required, the integrated level is high, excellent in use effect.
The method for detecting the behavioural exercise capacity of the multifunctional integrated cage for the non-human primate experimental animal comprises the following steps of:
(1) Feeding the experimental animals to be detected in the cage body in advance for one meal;
(2) Setting up a video recorder, wherein the maximum image pickup picture is in the range of the background plate;
(3) The adjustable shelf is adjusted to have a telescopic length relative to the socket according to the requirement, the locking bolt is tightened for fixation, and then the long food is fully inserted into the food jacks on the hanging shelf and the adjustable shelf respectively;
(4) After video recording is started, the hanging rack and the hanging rack are correspondingly inserted into the hanging rack fixing seat and the hanging rack fixing seat respectively, and then a technician immediately leaves the periphery of the cage body (in order to shape an environment which does not interfere with the surrounding or affect the heart state of the experimental animal, so as to promote the experimental animal to more naturally and objectively present the behavioural actions of limbs, joints and the like of the experimental animal, and be beneficial to more objectively collecting required data);
(5) The animal to be tested naturally shows the actions of limb coordination, climbing, arm pull-up and grabbing under the attraction of the long-strip food, so that the real movement capability of joints and fingers of the animal to be tested (such as finger flexibility or upper limb stationary tremors of the animal model for grabbing food in different areas and different test situations of a diabetic monkey or senile dementia) is known, and accurate and reliable hand information is provided for objective scientific evaluation.
Claims (6)
1. The utility model provides a multi-functional integrated cage for non-human primate experimental animals which characterized in that: the animal feeding and discharging gate comprises a cage body, wherein an animal feeding and discharging gate is arranged on the left side face of the cage body, the front side face of the animal feeding and discharging gate is a transparent plate, the rear side face of the animal feeding and discharging gate is a background plate, and a transverse holding rod area, a longitudinal holding rod area and an oblique holding rod area are arranged on the top face of the animal feeding and discharging gate; the transverse holding rod area consists of a plurality of holding rods which are transversely distributed; the longitudinal holding rod area consists of a plurality of holding rods which are longitudinally distributed; the oblique holding rod area consists of a plurality of holding rods which are distributed obliquely; the top surface is also detachably provided with hanging rack components; the left side surface and the right side surface of the cage body are respectively provided with a plurality of feeding ports; an adjustable hanger component is also distributed on the outer side of the feeding port;
the rack assembly comprises two rack fixing seats and a rack; a plurality of fixing holes are formed in the bottom plate of the rack fixing seat, and a fixing slot is formed in the middle of the bottom plate; the article hanging rack is 7-shaped as a whole, and the inclined edge of the article hanging rack is sequentially provided with a first layer inclined plate, a horizontal layer plate and a second layer inclined plate from bottom to top; the first layer inclined plate and the horizontal layer plate and the second layer inclined plate are respectively provided with a plurality of food insertion holes; the two ends of the object hanging rack are correspondingly inserted into the object hanging rack fixing seat to realize detachable fixing; the two hanging rack fixing seats are respectively fixed on the left side and the right side of the top surface of the cage body;
the adjustable hanging rack assembly comprises a hanging rack fixing seat and a hanging rack; the middle part of the hanging rack fixing seat is provided with a longitudinal slot; fixing holes are formed in two sides of the longitudinal slot; the object hanging rack comprises a 7-shaped socket and a 7-shaped adjusting rack; the sockets are detachably distributed in the longitudinal slots; a locking bolt is further arranged on the upper side of the socket; an adjusting long groove is formed on the inner side plate surface of the adjusting storage rack corresponding to the locking bolt; the adjusting long grooves are inserted into the locking bolts to be movably distributed and are pressed and fixed by the locking bolts; the outer side plate surface of the adjusting storage rack is provided with a plurality of food jacks along the inner and outer directions.
2. A multi-functional integrated cage for non-human primate experiments as claimed in claim 1 wherein: the upper plate surface of the socket is also provided with scales, and the scales are distributed corresponding to the adjusting long grooves.
3. A multi-functional integrated cage for non-human primate experiments as claimed in claim 1 wherein: the background plate is a milky white background plate, and an LED lamp strip is further arranged in the background plate.
4. A multi-functional integrated cage for non-human primate experiments as claimed in claim 1 wherein: the width and height dimensions of the background plate are larger than those of the front side surface.
5. A multi-functional integrated cage for non-human primate experiments as claimed in claim 1 wherein: the animal access gate is also correspondingly provided with a memory test board fixing slot.
6. A method for testing behavioural movement ability using a multifunctional integrated cage for non-human primate experimental animals as claimed in any one of claims 1 to 5, comprising the steps of:
(1) Feeding the experimental animals to be detected in the cage body in advance for one meal;
(2) Setting up a video recorder, wherein the maximum image pickup picture is in the range of the background plate;
(3) The adjustable shelf is adjusted to have a telescopic length relative to the socket according to the requirement, the locking bolt is tightened for fixation, and then the long food is fully inserted into the food jacks on the hanging shelf and the adjustable shelf respectively;
(4) After video recording is started, the object hanging frame and the object hanging frame are respectively inserted into the object hanging frame fixing seat and the object hanging frame fixing seat correspondingly, and then a technician immediately leaves the periphery of the cage body;
(5) The animal to be tested naturally shows the actions of limb coordination, climbing, arm pull-up and grabbing under the attraction of the long-strip food, so that the real movement capability of joints and fingers of the animal to be tested is known.
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| CN202110526594.XA CN113142073B (en) | 2021-05-14 | 2021-05-14 | Multifunctional integrated cage for non-human primate experimental animals and use method thereof |
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| CN114051943B (en) * | 2021-11-08 | 2023-04-07 | 广东春盛生物科技发展有限公司 | Non-human primate memory ability evaluation system |
| CN115191363B (en) * | 2022-07-12 | 2023-05-26 | 广西医科大学第一附属医院 | Joint movement testing system and method for experimental animals |
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| CN2759154Y (en) * | 2004-09-23 | 2006-02-22 | 首都医科大学北京神经科学研究所 | Combined type quadrumana experimental cage |
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| CN107047339A (en) * | 2017-03-29 | 2017-08-18 | 袁超 | A kind of medical experiment monkey class rearging cage |
| CN210113910U (en) * | 2019-04-01 | 2020-02-28 | 广东蓝岛生物技术有限公司 | Special cage suitable for experimental animal social behavior assessment |
| CN111616064A (en) * | 2020-06-23 | 2020-09-04 | 深圳先进技术研究院 | Animal conditioned position preference experimental device |
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| US4685424A (en) * | 1986-08-14 | 1987-08-11 | Bernard Migler | Pole-"housing" for monkeys |
| CN2759154Y (en) * | 2004-09-23 | 2006-02-22 | 首都医科大学北京神经科学研究所 | Combined type quadrumana experimental cage |
| CN204742149U (en) * | 2015-05-29 | 2015-11-11 | 苏州猴皇动物实验设备科技有限公司 | Monkey cage for animal experiments |
| CN107047339A (en) * | 2017-03-29 | 2017-08-18 | 袁超 | A kind of medical experiment monkey class rearging cage |
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