CN114051943B - Non-human primate memory ability evaluation system - Google Patents
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Abstract
The invention discloses a non-human primate memory capability evaluation system, which comprises an adaptive feeding stage experiment, a memory capability evaluation experiment and an experimental device, wherein the adaptive feeding stage experiment and the memory capability evaluation experiment are completed based on the cooperation of the experimental device, the experimental device comprises a lifting table and a cage, the cage is used for providing necessary living environment for an experimental animal, the experimental time of the adaptive feeding stage is 10-30 days, the adaptive feeding stage experiment has the function of enabling the experimental animal to adapt to the living environment and feed and can know an experimenter, the experimental time of the memory capability evaluation experiment is 30-60 days, an experimental object of the memory capability evaluation experiment should pass through the adaptive feeding stage experiment, and the experimenter performs a delayed response experiment on the experimental animal and evaluates the memory capability of the experimental animal. The evaluation system provided by the invention can be used for evaluating the short-term, long-term and overload memory abilities of the non-human primates respectively, and meanwhile, the experimental device is simple in structure, and the manufacturing cost is saved.
Description
Technical Field
The invention relates to the field of research on memory capacity of non-human primates, in particular to a memory capacity evaluation system of the non-human primates.
Background
The non-human primates belong to mammalians and primates, refer to primates with many biological characteristics similar to human beings, have high degrees of evolution, and are important experimental animals, wherein macaques are most used, and the non-human primates have many biological characteristics similar to human beings, such as miscellaneous food, more developed intelligence, nerve control, capability of manipulating tools by hands, common characteristics of common mammals, incapability of synthesizing vitamin C in vivo, and need to be taken from food. For example, macaque has 75% -98.5% homology with human genetic material, is an ideal animal model for human diseases and basic research, and is commonly used for research in the fields of infectious diseases, genetic diseases, cardiovascular diseases, behaviourology, neurobiology, reproductive physiology, pharmacology and toxicology, oncology and the like.
The non-human primate animal model is quite similar to human beings in biological evolution, anatomical structure (particularly brain structure) and physiological function, and compared with rodent experimental animals, the pharmacological or toxicological experimental results obtained by using the non-human primate animal model can better analize the body reflection of human beings after the administration of the drug, so that the non-human primate animal model has indispensable important roles in the aspects of researching human disease mechanisms, developing novel drugs and the like.
In the aspect of evaluating non-human primate animal models of human nervous system degenerative diseases such as Parkinson's disease, alzheimer's disease and the like, behavioral experimental data are very critical and can visually reflect the quality of model animals. Most of the conventional behavioral experimental devices aim at small experimental animals such as rodents, such as a mouse water maze learning memory test platform with the publication number of CN205124681U, the test platform can only aim at the small animals such as the rodents and cannot be used for evaluating the memory capacity of the non-human primates, but the conventional assessment device equipment for the non-human primates, such as an automatic device for evaluating the memory function of the primate experimental animals with the publication number of CN104663490B and an animal cognition evaluation method, has the problems of high manufacturing cost, long domestication period before experiments and the like, but part of simple devices, such as a non-human primate cognition and motion behavior test device with the publication number of CN204192622U, cannot obtain critical delayed reaction experimental data and cannot evaluate the memory capacity of the animals.
Disclosure of Invention
In order to solve the technical problems, the invention provides a memory ability evaluation system for a non-human primate, which comprises an adaptive feeding stage experiment, a memory ability evaluation experiment and an experimental device, wherein the adaptive feeding stage experiment and the memory ability evaluation experiment are completed based on the cooperation of the experimental device, the experimental device comprises a lifting table and a cage, the cage is arranged at the top of the lifting table and is used for providing a necessary living environment for an experimental animal, the experimental time duration of the adaptive feeding stage experiment is 10-30 days, in the adaptive feeding stage experiment, an experimenter normally feeds the experimental animal to enable the experimental animal to adapt to the living environment and feed and can know the experimenter, the time duration of the memory ability evaluation experiment is 30-60 days, the experimental object of the memory ability evaluation experiment is the experimental animal which passes the adaptive feeding stage experiment, and in the memory ability evaluation experiment, the experimenter performs a delayed response experiment on the experimental animal to evaluate the memory ability of the experimental animal.
Specifically, one side of the cage is provided with a tray, a plurality of hampers are detachably mounted at the top of the tray, the shape of each hamper is consistent, the tray is used for enabling the hampers to move back and forth relative to the cage, and the hampers are used for placing food for experiments.
Specifically, the hamper is opaque, and the number of hamper sets up to 4 at least, and each top of hamper all is provided with the lid.
Preferably, the hamper can be changed different colours or adopt different hamper arrangement modes according to the experiment needs, can also set up different opening modes to the hamper.
Preferably, the tray can be horizontally or vertically arranged according to the experiment requirement.
Specifically, a window is arranged on one side of the cage close to the tray, and the window is used for enabling the experimental animal to take the food in the food box.
Specifically, be provided with opaque baffle between cage utensil and the tray, the baffle can reciprocate for cage utensil, the baffle is used for separating cage utensil and tray, just the baffle makes the laboratory animal in the cage utensil can't see the tray.
Specifically, one side of the cage is provided with a video capturing device, the video capturing device comprises a camera and a video camera, and the video capturing device is used for monitoring and recording the behaviors of the experimental animals.
Specifically, the adaptive feeding phase experiment comprises the following steps:
1) Before the experiment begins, the experimental animals are given 50% of the normal food or are not fed in the night before the experiment;
2) The experimenter pulls the baffle open, marks the hamper and moves the hamper to a position which can not be reached by the experimental animal;
3) At the beginning, the interval between the experimenter and the experimental animal is more than 0.5m, and 1 part of food loved by the experimental animal is randomly put into one of the hampers under the condition that the experimental animal can clearly see the operation of the experimenter;
4) Moving the tray to a position where the experimental animal can touch, withdrawing the experimenter to 2m, observing and recording the number of the hamper touched by the experimental animal, and recording the result if the experimental animal does not take food before;
5) Collecting and collating experimental data, and taking the experimental data as data of delay time 0 s;
6) Repeating the steps 1) to 5) for 4 times every day for each experimental animal, and arranging 2 days for experimental operation every week;
7) Judging the effect of the experiment in the adaptive feeding stage, if the experimental animal can sit well after the experiment starting signal is sent out, and can wait for the experiment in the face of the experimenter, the effect of the experiment in the adaptive feeding stage is good, and the memory ability evaluation experiment can be entered.
Specifically, in the step 3), randomly putting one of the boxes refers to determining the number of the box for putting food by using a random number table.
Specifically, the memory ability evaluation test comprises the following steps:
a) Completing the experimental operations of the steps 1) to 3);
b) The baffle plate is immediately placed back after food is placed, the experimental animal is separated from the hamper, countdown is started according to the experimental needs, and the baffle plate is immediately pulled up after the countdown is finished;
c) Moving the tray to a position where the experimental animal can touch the hamper, withdrawing an experimenter to 2m, observing and recording the hamper number touched by the experimental animal, and recording the result if the experimental animal does not take food before;
d) Collecting and collating experimental data, and taking the experimental data as data of delay time;
e) Each animal was repeated 4 times daily for each of the experimental animals, and the experimental procedures were performed 2 days per week.
Specifically, in step B), the times of the countdown are set to 5s, 10s, 15s, 30s, and 60s.
Compared with the prior art, the invention has the beneficial effects that:
1. through the arrangement of the lifting platform in the experimental device, the lifting platform can adapt to different cage heights, so that experimental animals can be detected in the native cage, and the stress reaction of the animals caused by the replacement of the living environment is avoided;
2. by combining the adaptive feeding stage experiment, the memory capacity evaluation experiment and the experimental device, the short-term, long-term and overload memory capacities of the non-human primates can be evaluated while the manufacturing cost is controlled.
Drawings
FIG. 1 is a schematic view of the overall structure of an experimental apparatus of the present invention;
FIG. 2 is a flow chart of an experiment of the adaptive feeding phase of the present invention;
FIG. 3 is a flow chart of a memory assessment experiment according to the present invention;
in the figure, 1, a lifting platform; 2. a cage; 3. a tray; 4. a food box; 5. a box cover; 6. a window; 7. a baffle plate; 8. a video capture device.
Detailed Description
In the following, the technical solutions of the present invention will be described clearly and completely, it should be noted that, in the following description, the terms "front", "back", "left", "right", "upper" and "lower" refer to directions in fig. 1, the terms "bottom" and "top", "inner" and "outer" refer to directions toward or away from a specific component geometry, respectively, and furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be interpreted as indicating or implying relative importance or implicitly indicating the number of indicated technical features, the term "plurality" means two or more, and the terms "mounted", "connected", "fixed" and the like are to be interpreted broadly, for example, "connected" may be fixedly connected, detachably connected, or integrally connected; "coupled" may be direct or indirect through an intermediary. The specific meanings of the above terms in the disclosed embodiments of the invention can be understood by those of ordinary skill in the art as appropriate. It should be apparent that the described embodiments are only some of the embodiments of the present invention, and not all of them. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Materials, instruments, reagents and the like used in the following examples are commercially available unless otherwise specified. The technical means used in the examples are conventional means well known to those skilled in the art, unless otherwise specified.
Example one
As shown in fig. 1, the experimental device comprises an elevating platform 1 and a cage 2, the cage 2 is arranged on the top of the elevating platform 1, the cage 2 is used for providing necessary living environment for experimental animals, a tray 3 is arranged on one side of the cage 2, a plurality of food boxes 4 are detachably mounted on the top of the tray 3, the shape of each food box 4 is consistent, the tray 3 is used for enabling the food boxes 4 to move back and forth relative to the cage 2, the food boxes 4 are used for placing food for experiments, the food boxes 4 are opaque food boxes 4, and a box cover 5 is arranged on the top of each food box 4, so that the experimental animals cannot see the food from the outside of the food boxes 4, the food boxes 4 containing the food can be found only through self-memory, the number of the food boxes 4 is set to be at least 4, the number of the food boxes 4 can be increased to 4-10 according to the needs of experiments, or the color of the food boxes 4 and the opening mode of the box cover 5 can be changed according to the needs, thereby testing the memory load of the experimental animals.
One side that cage utensil 2 is close to tray 3 is provided with window 6, window 6 is used for making the experimental animal can take the food in the hamper 4, be provided with opaque baffle 7 between cage utensil 2 and the tray 3, baffle 7 can reciprocate for cage utensil 2, baffle 7 is used for separating cage utensil 2 and tray 3, and baffle 7 makes the experimental animal in the cage utensil 2 can't see tray 3, when the experiment goes on, baffle 7 is in the state of rising, thereby let the experimental animal can observe the operation of experimenter and form corresponding memory, the test stage, the experimenter should put down baffle 7 immediately after putting food, make the experimental animal can't observe the food position, thereby accomplish the test according to the memory of experimental animal.
One side of the cage 2 is provided with a video capturing device 8, the video capturing device 8 comprises a camera and a video camera, and the video capturing device 8 is used for monitoring and recording the behaviors of the experimental animals.
As shown in fig. 2, which is a schematic flow chart of the experiment in the adaptive feeding stage, the experiment duration in the adaptive feeding stage is 10-30 days, and in the experiment in the adaptive feeding stage, the experimenter normally feeds the experimental animal to adapt the experimental animal to the living environment and the feed, and can know the experimenter, the specific steps are as follows:
1) Before the experiment begins, the experimental animals are given 50% of the normal food or are not fed in the night before the experiment;
2) The experimenter pulls the baffle 7 open, marks the hamper 4 and moves the hamper 4 to a position which can not be reached by the experimental animal;
3) At the beginning, the distance between the experimenter and the experimental animals is more than 0.5m, under the condition that the experimenter can clearly see the operation of the experimenter, 1 part of favorite food of the experimental animals is randomly thrown into one of the food boxes 4, and the random throwing into one of the food boxes 4 means that the random number table is used for determining the number of the food box 4 into which the food is thrown;
4) Moving the tray 3 to a position where the experimental animal can touch, withdrawing the experimenter to 2m away, observing and recording the number of a hamper 4 touched by the experimental animal at first, and recording the result if the experimental animal does not take food before;
5) Collecting and sorting experimental data, and taking the experimental data as data of delay time 0 s;
6) Repeating the steps 1-5 for 4 times every day for each experimental animal, and arranging 2 days every week for experimental operation;
7) Judging the effect of the experiment in the adaptive feeding stage, if the experimental animal can sit well after the experiment starting signal is sent out, and can wait for the experiment in the face of the experimenter, the effect of the experiment in the adaptive feeding stage is good, and the memory ability evaluation experiment can be entered.
When the effect of the adaptive stage feeding experiment is good, a memory ability evaluation experiment can be performed, as shown in fig. 3, the memory ability evaluation experiment is a schematic flow chart of the memory ability evaluation experiment, the duration of the memory ability evaluation experiment is 30-60 days, the experimental subject of the memory ability evaluation experiment is an experimental animal which passes the adaptive feeding stage experiment, in the memory ability evaluation experiment, an experimenter performs a delayed response experiment on the experimental animal to evaluate the memory ability of the experimental animal, and the adaptive stage feeding experiment comprises the following steps:
a) Completing the experimental operations of step 1) -step 3) of the adaptive stage feeding experiment;
b) The baffle 7 is immediately placed back after food is placed, the experimental animal is separated from the hamper 4, countdown is started according to the experimental requirement, the countdown time is set to be 5s, 10s, 15s, 30s and 60s, and the baffle 7 is immediately pulled up after the countdown is finished;
c) Moving the tray 3 to a position where the experimental animal can touch the hamper 4, withdrawing the experimenter to 2m away, observing and recording the number of the hamper 4 touched by the experimental animal, and recording the result if the experimental animal does not take food before;
d) Collecting and collating experimental data, and taking the experimental data as data of delay time;
e) Each animal was repeated 4 times daily for each of the experimental animals, and the experimental procedures were performed 2 days per week.
In memory ability evaluation experimental stage, change the time of count-down, can test experimental animals ' short-term memory and long-term memory, through the opening mode that changes colour, arrangement mode and lid 5 of hamper 4, can also test experimental animals's memory load, combine video capture device 8, can also be with the complete record of experimental animals's action to be favorable to perfecting or proofreading and correct experimental data.
In summary, the above embodiments are merely preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (7)
1. The system is characterized by comprising an adaptive feeding stage experiment, a memory ability evaluation experiment and an experimental device, wherein the adaptive feeding stage experiment and the memory ability evaluation experiment are completed based on the cooperation of the experimental device, the experimental device comprises a lifting table (1) and a cage (2), the cage (2) is arranged at the top of the lifting table (1), the cage (2) is used for providing a necessary living environment for an experimental animal, the experimental time of the adaptive feeding stage experiment is 10-30 days, in the adaptive feeding stage experiment, an experimenter normally feeds the experimental animal to adapt the experimental animal to the living environment and feed, and can know the experimenter, the experimental time of the memory ability evaluation is 30-60 days, an experimental object of the memory ability evaluation experiment is an experimental animal which passes the adaptive feeding stage experiment, and in the memory ability evaluation experiment, the experimenter performs a delayed reaction experiment on the experimental animal to evaluate the memory ability of the animal;
a tray (3) is arranged on one side of the cage (2), a plurality of hampers (4) are detachably mounted on the top of the tray (3), the shape of each hamper (4) is consistent, the tray (3) is used for enabling the hampers (4) to move back and forth relative to the cage (2), the hampers (4) are used for placing food used for experiments, an opaque baffle (7) is arranged between the cage (2) and the tray (3), the baffle (7) can move up and down relative to the cage (2), the baffle (7) is used for separating the cage (2) and the tray (3), and the baffle (7) prevents experimental animals in the cage (2) from seeing the tray (3);
the memory ability evaluation experiment comprises the following steps:
a) Before the experiment begins, the experimental animals are given 50% of the normal food or are not fed in the night before the experiment; the experimenter pulls the baffle (7) open, marks the hamper (4) and moves the hamper (4) to a position which can not be reached by the experimental animal; at the beginning, the interval between the experimenter and the experimental animal is more than 0.5m, and 1 part of food loved by the experimental animal is randomly put into one of the hampers (4) under the condition that the experimental animal can clearly see the operation of the experimenter;
b) The baffle (7) is put back immediately after food is placed, the experimental animal is separated from the hamper (4), countdown is started according to the experimental needs, and the baffle (7) is pulled up immediately after the countdown is finished;
c) Moving the tray (3) to a position where the experimental animal can touch the hamper (4), withdrawing the experimenter to 2m, observing and recording the number of the hamper (4) touched by the experimental animal, and recording the result if the experimental animal does not take food before;
d) Collecting and collating experimental data, and taking the experimental data as data of delay time;
e) Each animal was repeated 4 times daily with 2 days of each week.
2. The non-human primate memory ability evaluation system according to claim 1, wherein the food boxes (4) are opaque food boxes (4), and the number of the food boxes (4) is set to at least 4, and a box cover (5) is provided on the top of each of the food boxes (4).
3. The system for assessing the memory of non-human primates according to claim 1, wherein the cage (2) is provided with a window (6) on the side close to the tray (3), and the window (6) is used for enabling the experimental animal to access the food in the hamper (4).
4. The non-human primate memory ability evaluation system according to claim 1, wherein one side of the cage (2) is provided with a video capturing device (8), the video capturing device (8) comprising a camera and a video camera, the video capturing device (8) being used for monitoring and recording the behavior of the laboratory animal.
5. The non-human primate memory assessment system according to claim 1 wherein the adaptive feeding phase experiment comprises the steps of:
1) Before the experiment begins, the experimental animals are given 50% of the normal food or are not fed in the night before the experiment;
2) The experimenter pulls the baffle (7) open, marks the hamper (4) and moves the hamper (4) to a position which can not be reached by the experimental animal;
3) At the beginning, the interval between the experimenter and the experimental animal is more than 0.5m, and 1 part of food loved by the experimental animal is randomly put into one of the hampers (4) under the condition that the experimental animal can clearly see the operation of the experimenter;
4) Moving the tray (3) to a position where the experimental animal can touch, withdrawing the experimenter to 2m, observing and recording the number of a hamper (4) touched by the experimental animal firstly, and recording the result if the experimental animal does not take food before;
5) Collecting and collating experimental data, and taking the experimental data as data of delay time 0 s;
6) Repeating the steps 1) to 5) for 4 times every day by each experimental animal, and arranging 2 days every week for experimental operation;
7) Judging the effect of the experiment in the adaptive feeding stage, if the experimental animal can sit well after the experiment starting signal is sent out, and can wait for the experiment in the face of the experimenter, the effect of the experiment in the adaptive feeding stage is good, and the memory ability evaluation experiment can be entered.
6. The system for evaluating memory ability of a non-human primate according to claim 5, wherein in the step 3), the randomly putting one of the trays (4) is to determine a number of the tray (4) to which food is put using a random number table.
7. The system for evaluating memory ability of a non-human primate according to claim 1, wherein the time for counting down in step B) is set to 5s, 10s, 15s, 30s and 60s.
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| CN103598144B (en) * | 2013-12-05 | 2015-05-20 | 昆明医科大学 | Tree shrew behavioristic evaluation method |
| CN103704173A (en) * | 2013-12-12 | 2014-04-09 | 内蒙古医科大学 | Method of testing scatter-hoarding behavior in rodents |
| CN104663490B (en) * | 2015-03-03 | 2018-10-09 | 广西南宁灵康赛诺科生物科技有限公司 | The automatic device of a set of evaluation primate laboratory animal memory function and the cognitive ability evaluation method of animal |
| US20170000906A1 (en) * | 2015-06-30 | 2017-01-05 | Mousera, Inc | System and method of measuring efficacy of cancer therapeutics |
| CN111685057B (en) * | 2020-07-14 | 2021-11-09 | 北京大学第六医院 | Device and method for training and testing working memory capacity of rodent |
| CN113142073B (en) * | 2021-05-14 | 2023-05-30 | 广西盛思蓝生物医药技术有限公司 | Multifunctional integrated cage for non-human primate experimental animals and use method thereof |
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