CN114916452A - Device for testing influence of IVC environmental parameters on thermal comfort of SPF experimental animals - Google Patents
Device for testing influence of IVC environmental parameters on thermal comfort of SPF experimental animals Download PDFInfo
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- CN114916452A CN114916452A CN202210586435.3A CN202210586435A CN114916452A CN 114916452 A CN114916452 A CN 114916452A CN 202210586435 A CN202210586435 A CN 202210586435A CN 114916452 A CN114916452 A CN 114916452A
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- 238000012360 testing method Methods 0.000 title claims abstract description 144
- 238000010171 animal model Methods 0.000 title claims abstract description 33
- 230000007613 environmental effect Effects 0.000 title claims abstract description 24
- 208000019901 Anxiety disease Diseases 0.000 claims abstract description 27
- 230000036506 anxiety Effects 0.000 claims abstract description 27
- 230000033001 locomotion Effects 0.000 claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 241001465754 Metazoa Species 0.000 claims description 15
- 230000009965 odorless effect Effects 0.000 claims description 8
- 230000000386 athletic effect Effects 0.000 claims 1
- 230000008520 organization Effects 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 21
- 230000003542 behavioural effect Effects 0.000 abstract description 6
- 241000699670 Mus sp. Species 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 238000005192 partition Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000009967 tasteless effect Effects 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/027—Exercising equipment, e.g. tread mills, carousels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The invention discloses a device for testing the influence of IVC environmental parameters on the thermal comfort of SPF-level experimental animals, which comprises a box body, a depression testing chamber, a plurality of light sources, a learning and memory testing chamber, a movement capability testing chamber, a plurality of cameras, an anxiety testing chamber and a computer, wherein the depression testing chamber, the learning and memory testing chamber, the movement capability testing chamber and the anxiety testing chamber are arranged in the box body, the light sources and the cameras are respectively arranged in the depression testing chamber, the learning and memory testing chamber and the movement capability testing chamber, the light sources and the cameras are respectively connected with the computer, the depression testing mechanism, the learning and memory testing layer, the movement capability testing mechanism and the anxiety testing mechanism are respectively arranged in the depression testing chamber, the learning and memory testing chamber, the movement capability testing chamber and the anxiety testing chamber, compared with the prior art, the device is researched based on a behavioral experiment, and can more comprehensively and accurately reflect the influence of environmental factors on the experimental animals, and the structure is simple, and the operation is convenient.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of experimental animal behavioural experiment testing devices, in particular to a device for testing the influence of IVC (in-vitro fertilization) environmental parameters on the thermal comfort of SPF (specific pathogen free) experimental animals.
[ background ] A method for producing a semiconductor device
SPF (Specific Pathogen Free, SPF) grade laboratory animals, i.e. Pathogen-Free animals, are laboratory animals which, apart from the pathogens which the cleaning animals are supposed to exclude, do not carry pathogens which are primarily potentially pathogenic for infections or conditions and which are highly disruptive to scientific experiments, and which are Free of Specific microorganisms and parasites in their organism, but which are not tolerated. The SPF experimental animal has certain adaptive capacity to the thermal environment, if the thermal environment is slightly uncomfortable, the experimental animal can also tolerate and adapt to a period of time, but the good thermal comfortable environment is more favorable for the growth of the animal, and is convenient for the experimental research of the animal. Meanwhile, the experimental animals do not have the language specific to human beings, and the comfortable growth temperature and humidity range of the experimental animals are difficult to determine. Therefore, how to determine a thermal comfort environment suitable for the growth of the experimental animal is very critical.
[ summary of the invention ]
The invention aims to solve the problems in the prior art, provides a device for testing the thermal comfort influence of IVC environmental parameters on SPF experimental animals, can more comprehensively and accurately reflect the influence of environmental factors on the experimental animals by researching based on behavioral experiments, and has the advantages of simple structure and convenient operation.
In order to achieve the purpose, the invention provides a device for testing the influence of IVC environmental parameters on the thermal comfort of SPF-level experimental animals, which comprises a box body, a depression testing chamber, a plurality of light sources, a learning and memory testing chamber, a movement ability testing chamber, a plurality of cameras, an anxiety testing chamber and a computer, wherein the depression testing chamber, the learning and memory testing chamber, the movement ability testing chamber and the anxiety testing chamber are arranged in the box body, the light sources and the cameras are respectively arranged in the depression testing chamber, the learning and memory testing chamber and the movement ability testing chamber, the light sources and the cameras are respectively connected with the computer, and the depression testing mechanism, the learning and memory testing layer, the movement ability testing mechanism and the anxiety testing mechanism are respectively arranged in the depression testing chamber, the learning and memory testing chamber, the movement ability testing chamber and the anxiety testing chamber.
Preferably, the inner space of the box body is divided into an upper space and a lower space by a partition board, the upper space is a depression testing room, the lower space is divided into a learning memory testing room, a motor ability testing room and an anxiety testing room by partition boards, different behavioristic experimental devices are placed in different testing rooms, and instruments are not interfered with one another.
Preferably, the depression testing mechanism comprises a reservoir, a water inlet pipe and a water discharge pipe are arranged on the reservoir, a water inlet valve and a water discharge valve are respectively arranged on the water inlet pipe and the water discharge pipe, and the water inlet valve and the water discharge valve can realize water supply and water discharge functions.
Preferably, the learning and memory test layer comprises a layer of wood shaving bedding material and an odorless tissue-towel sheet evenly laid on the layer of wood shaving bedding material.
Preferably, the exercise capacity testing mechanism comprises a plurality of supporting columns and transverse plates arranged on the supporting columns.
Preferably, the anxiety test room comprises a bright box and a dark box arranged at one end of the bright box, the bright box is communicated with the dark box through a shuttle hole, and a light source and a camera which are connected with a computer are arranged in the bright box and the dark box.
Preferably, a timer is arranged at the upper end of the box body, so that the time recording work of the behavioural experiment is facilitated.
Preferably, the lower end of the box body is provided with a plurality of movable wheels, so that the whole testing device can be conveniently moved.
Preferably, the light source is an adjustable light source, and different illumination requirements of a behavior experiment can be met.
The invention has the beneficial effects that: according to the invention, four different ethological experiments are carried out on the SPF experimental animals under different environmental parameters, and whether the environments of the SPF experimental animals are healthy or not is judged and analyzed, so that the influence of the IVC environmental parameters on the thermal comfort of the SPF experimental animals is obtained.
The features and advantages of the present invention will be described in detail by embodiments with reference to the accompanying drawings.
[ description of the drawings ]
FIG. 1 is a schematic structural diagram of a device for testing the influence of IVC environmental parameters on the thermal comfort of SPF-grade experimental animals according to the present invention;
FIG. 2 is a partial side view of a device for testing the thermal comfort effect of IVC environmental parameters on SPF-grade laboratory animals in accordance with the present invention;
fig. 3 is a schematic diagram of an anxiety testing mechanism.
In the figure: the test system comprises a box body 1, a timer 2, a depression test chamber 3, a water storage tank 4, a light source 5, a drain valve 6, a drain pipe 7, a water inlet valve 8, a water inlet pipe 9, a learning and memory test chamber 10, a motor ability test chamber 11, a moving wheel 12, a camera 13, a light box 14, a dark box 15, a transverse plate 16, an anxiety test chamber 17, a CAN field bus 18, a computer 19, a shaving cushion layer 20, a tasteless tissue paper towel 21, a support column 22, a partition plate 23 and a shuttling hole 24.
[ detailed description ] embodiments
This example illustrates the principles of the present invention by way of anxiety testing via light box 14 and dark box 15 experiments, exercise performance testing via cross board balance experiments, learning and memory testing via wood shavings bedding layer 20 and odorless tissue towel sheet 21 nesting experiments, and depression testing via reservoir forced swimming experiments
Referring to fig. 1, 2 and 3, the device for testing influence of IVC environmental parameters on thermal comfort of SPF-level experimental animals comprises a box body 1, a depression testing chamber 3, a plurality of light sources 5, a learning and memory testing chamber 10, a movement ability testing chamber 11, a plurality of cameras 13, an anxiety testing chamber 17 and a computer 19, wherein the depression testing chamber 3, the learning and memory testing chamber 10, the movement ability testing chamber 11 and the anxiety testing chamber 17 are arranged in the box body 1, the light sources 5 and the cameras 13 are arranged in the depression testing chamber 3, the learning and memory testing chamber 10 and the movement ability testing chamber 11, the light sources 5 and the cameras 13 are connected with the computer 19, the depression testing mechanism, the learning and memory testing chamber 10, the movement ability testing chamber 11 and the anxiety testing chamber 17 are respectively provided with a depression testing mechanism, a learning and memory testing layer, a movement ability testing mechanism and an anxiety testing mechanism, the inner space of the box body 1 is divided into an upper layer space and a lower layer space by a partition plate 23, the upper layer space is a depression testing chamber 3, the lower layer space is divided into a learning memory testing chamber 10, a movement ability testing chamber 11 and an anxiety testing chamber 17 by the partition plate 23, the depression testing mechanism comprises a reservoir 4, a water inlet pipe 9 and a water outlet pipe 7 are arranged on the reservoir 4, a water inlet valve 8 and a water outlet valve 6 are respectively arranged on the water inlet pipe 9 and the water outlet pipe 7, the learning memory testing chamber comprises a shaving cushion layer 20 and an odorless tissue paper piece 21 uniformly tiled on the shaving cushion layer 20, the movement ability testing mechanism comprises a plurality of supporting columns 22 and transverse plates 16 arranged on the supporting columns 22, the anxiety testing chamber 17 comprises a bright box 14 and a dark box 15 arranged at one end of the bright box 14, the bright box 14 is communicated with the dark box 15 through a shuttle hole 24, and a light source 5 and a camera 13 connected with a computer 19 are also arranged in the bright box 14 and the dark box 15, the upper end of the box body 1 is provided with the timer 2, the lower end of the box body 1 is provided with the plurality of movable wheels 12, the light source 5 is an adjustable light source, the experimental process recorded by the camera 13 CAN be observed through the computer 19, and the CAN field bus 18 is used for receiving the instruction sent by the software of the computer 19.
The working process of the invention is as follows:
according to the device for testing the influence of IVC environmental parameters on the thermal comfort of SPF experimental animals, a certain number of inbred male and female mice of four-week old are randomly distributed into different living environments in the working process, and are bred for 6 weeks to perform behavioral experiments. The anxiety testing mechanism experiment is used for testing the anxiety of the mouse, adopts the camera shooting video tracking technology to identify the situation of the target acquisition activity, and comprises the following steps: the adjustable light source 5 on the light box 14 is adjusted to the natural light brightness, the light box 14 is exposed to the natural light source, and the illumination intensity of the dark box 15 is adjusted to 2 lux. The mice were placed in the open box 14 with the head facing the shuttle aperture, freely explored for 10min, and acclimated twice. In the third experiment, the CAN field bus 18 receives an instruction sent by the software, the camera 13 collects the activity condition of the mouse, the image acquisition card converts the collected analog signal into a digital signal, and the software of the computer 19 processes and analyzes the transmitted digital signal.
The balance experiment is used for detecting the motion ability of the mouse, the width of the transverse plate 16 is 2cm, and a soft cushion is paved below the transverse plate 16. The test was performed for 3 consecutive days, with 2 days training for 1 day of testing. Mice were placed on one end of the transverse plate 16 and scored by stimulating the mice to walk across the transverse plate 16 and observing their passage. Scoring criteria for the experiment: can not stand for 0 minutes on the transverse plate 16; keeping the horizontal plate 16 still for 1 minute; attempt to pass 2 minutes of falling on the cross plate 16; the sliding wheel can smoothly pass through the transverse plate 16, but the sliding frequency exceeds 5 times and 3 minutes; the number of slipping times of the transverse plate 16 is more than 1 and less than 5 and 4 minutes; the number of slipping times of the transverse plate 16 is 1 time and 5 minutes; the total smooth passage through the transverse plate 16 is 6 minutes.
The nesting experiment is used for detecting the learning and memory capacity of a mouse, a shaving bedding layer 20 with the thickness of 1cm is placed in the learning and memory testing room 10, meanwhile, a square odorless thin tissue towel piece 21 with the thickness of 4.5cm is evenly paved on the shaving bedding layer 20, the mouse is placed in the learning and memory testing room 10, and the nesting condition of the mouse is observed after 2 hours and scored. The scoring criteria were as follows: 210 points of odorless tissue-towel sheets are completely not touched; the tasteless tissue-towel sheet 21 is scattered at all positions of the learning and memory testing room 10, and no bite mark is 1 point; the tasteless tissue-towel sheet 21 is gathered to one side of the learning and memory test room 10, but is loose without a formed nest and has no obvious bite mark of 2 points; the odorless tissue-towel sheet 21 is gathered and folded, a formed but relatively flat nest is visible, and part of the nest is bitten by 3 minutes; the odorless tissue-towel sheet 21 is torn into small pieces which are gathered together and folded into a deeper nest 4.
The forced swimming test is used for the depression detection of mice. A reservoir 4 is arranged in the depression testing chamber 3, before the experiment begins, a water inlet valve 8 is opened, a water inlet pipe 9 is connected with a water source, water is supplied into the reservoir 4 until the water depth is 15cm, and the water inlet valve 8 is closed after the water supply is finished. The total experiment time is 6min, the first 2min belongs to the adaptation stage, and the second 4min enters the test stage. The mice were placed in a reservoir 4 and after the acclimation phase, the time the mice stopped struggling in water or exhibited a floating state was observed and recorded. After the experiment is finished, the drain valve 6 is opened, and the water in the reservoir 4 is drained from the drain pipe 7.
The data of the four groups of behavioral experiments are processed and analyzed through computer software, and finally, a relevant conclusion that IVC environmental factors influence the thermal comfort of SPF-level experimental animals is obtained.
The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.
Claims (9)
- IVC environmental parameter is to the thermal comfort influence testing arrangement of SPF level experimental animals, its characterized in that: the device comprises a box body (1), a depression testing room (3), a plurality of light sources (5), a learning and memory testing room (10), a movement ability testing room (11), a plurality of cameras (13), an anxiety testing room (17) and a computer (19), wherein the depression testing room (3), the learning and memory testing room (10), the movement ability testing room (11) and the anxiety testing room (17) are arranged in the box body (1), the light sources (5) and the cameras (13) are arranged in the depression testing room (3), the learning and memory testing room (10) and the movement ability testing room (11), the light sources (5) and the cameras (13) are connected with the computer (19), and a depression testing mechanism, a learning and memory testing layer and a learning and memory testing layer are respectively arranged in the depression testing room (3), the learning and memory testing room (10), the movement ability testing room (11) and the anxiety testing room (17), A motor ability testing mechanism and an anxiety testing mechanism.
- 2. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-grade laboratory animals according to claim 1, characterized in that: the interior space of box (1) is separated into upper and lower two-layer space through baffle (23), and upper strata space is depression test room (3), and lower floor's space passes through baffle (23) and separates into learning memory test room (10), motor ability test room (11) and anxiety test room (17).
- 3. Device for testing the impact of IVC environmental parameters on the thermal comfort of SPF-class laboratory animals according to claim 1, characterized in that: depression accredited testing organization includes cistern (4), be equipped with inlet tube (9) and drain pipe (7) on cistern (4), be equipped with water intaking valve (8) and drain valve (6) on inlet tube (9) and drain pipe (7) respectively.
- 4. Device for testing the impact of IVC environmental parameters on the thermal comfort of SPF-class laboratory animals according to claim 1, characterized in that: the learning and memory test layer comprises a shaving bedding layer (20) and an odorless tissue towel sheet (21) which is evenly paved on the shaving bedding layer (20).
- 5. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-grade laboratory animals according to claim 1, characterized in that: the athletic ability testing mechanism comprises a plurality of supporting columns (22) and transverse plates (16) arranged on the supporting columns (22).
- 6. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-grade laboratory animals according to claim 1, characterized in that: the anxiety test chamber (17) comprises a bright box (14) and a dark box (15) arranged at one end of the bright box (14), the bright box (14) is communicated with the dark box (15) through a shuttle hole (24), and a light source (5) and a camera (13) which are connected with a computer (19) are arranged in the bright box (14) and the dark box (15).
- 7. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-grade laboratory animals according to claim 1, characterized in that: the upper end of the box body (1) is provided with a timer (2).
- 8. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-grade laboratory animals according to claim 1, characterized in that: the lower end of the box body (1) is provided with a plurality of movable wheels (12).
- 9. Device for testing the thermal comfort impact of IVC environmental parameters on SPF-class laboratory animals according to any one of claims 1 to 8, characterized in that: the light source (5) is an adjustable light source.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210586435.3A CN114916452A (en) | 2022-05-26 | 2022-05-26 | Device for testing influence of IVC environmental parameters on thermal comfort of SPF experimental animals |
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| CN202210586435.3A CN114916452A (en) | 2022-05-26 | 2022-05-26 | Device for testing influence of IVC environmental parameters on thermal comfort of SPF experimental animals |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6678413B1 (en) * | 2000-11-24 | 2004-01-13 | Yiqing Liang | System and method for object identification and behavior characterization using video analysis |
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Application publication date: 20220819 |