CN101851651A - Biosynthesis method for temperature-resistant dendritic slow-digestion starch - Google Patents
Biosynthesis method for temperature-resistant dendritic slow-digestion starch Download PDFInfo
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Abstract
The invention provides a biosynthesis method for temperature-resistant dendritic slow-digestion starch, which belongs to the technical field of non-chemically modified starch. The method utilizes a biological catalytic conversion technique for beta-amylase, amylomaltase, maltose-base amylase, chorismic amylase and transglucosylase to recombine starch molecular chain of different-source commercialized starch through synergistic modification of novel hydrolysis-transglycosylation, such as corn starch, wheat starch, rice starch, potato starch, cassava starch, sweet potato starch, mung bean starch, chickpea starch, sorghum starch, sago starch, banana taro starch and the like, thereby obtaining the temperature-resistant dendritic starch with the characteristic of slow digestion. The temperature-resistant dendritic slow-digestion starch obtained by the method can be used as a functional raw/auxiliary material to develop hypoglycemia healthy foods, energy slow-release foods, nutritional factor-targeted controlled-release carrier materials and the like.
Description
Technical field
What the present invention relates to is a kind of with the method for biological enzyme from the synthetic temperature-resistant dendritic slow-digestion starch of native starch, belongs to on-chemically modified starch technical field.
Background technology
Along with the variation of social structure, quickening, food habits and the food composition of rhythm of life change and social senilization, very big change has taken place in the human diseases spectrum, chronic disease, constantly rise as insulin resistant relative disease ratios such as diabetes, cardiovascular disorder, obesity, hyperlipidaemia, hypertension, become global great public health problem at present.Prevent the key that above-mentioned disease takes place to be scientific and reasonable diet formula.
(slowly digestible starch SDS) is meant that those are absorbed but slow starch by complete digestion to slow-digestion starch in small intestine.According to the classification of starch bioavailability, it can be defined as the starch nutrition segment of under in-vitro simulated digestion condition, being mixed enzyme (pancreatic, saccharifying enzyme and saccharase) digestion in 20～120min.As a kind of novel functional foodstuff, slowly digest and assimilate after SDS is edible, continuous energy release, keep the postprandial blood sugar stable state, while can be prevented and be treated various relevant chronic diseases with diet (as obesity, diabetes, glycogen storage disease and metabolic syndrome), thereby becomes a research focus in Food science and modern nutriology field day by day.At present, the research institution of SDS mainly concentrates on American-European countries, and relevant report only is confined to preparation method and physiological function research, and is domestic actually rare at this area research.And the SDS preparation method mainly adopts the pasted starch recrystallization handle to form physics attitude SDS, and this product is easily changing poor heat stability under certain processing conditions; Utilize the hydrolysis/commentaries on classics glycosides effect of amylase to modify the temperature-resistant dendritic SDS of starch molecular chain synthetic chemistry attitude, this product can satisfy the process operation of food and the demand that nutrition keeps, and has the vast market using value as a kind of novel functional foodstuff auxiliary material.
Summary of the invention
The object of the present invention is to provide a kind ofly be of high nutritive value, the biosynthetic means of nourishing function is good, glycemic index is low temperature-resistant dendritic slow-digestion starch.
Technical scheme of the present invention: a kind of biosynthetic means of temperature-resistant dendritic slow-digestion starch, with starch is raw material, utilize the biocatalysis transformation technology of beta-amylase, maltose glucosyltransferase, malt-base amylase, branch's amylase, transglucosidase, modify the reorganization starch molecular chain by multienzyme synergism hydrolysis-commentaries on classics glycosides, thereby obtain temperature-resistant dendritic slow-digestion starch; Technology is:
A) take by weighing the 1g raw starch and be made into the starch milk solution that mass concentration is 1%-50%, handle 2-30min temperature 80-100 ℃ of following boiling then, make its complete gelatinization;
B) treat that starch paste is cooled to room temperature, add the sodium-acetate buffer of 10-50mL pH 4.0-7.0 and the mixed enzyme solution of 100-5000U/g substrate, mixed enzyme solution is selected beta-amylase, maltose glucosyltransferase, malt-base amylase, branch's amylase, transglucosidase for use, optional two kinds of enzymes are formed, and the proportioning of two kinds of enzymes is volume ratio 1: 1～1: 100;
C) at temperature 30-90 ℃, pH 4.0-7.0 constantly stirs isothermal reaction 1-24h;
D) enzyme that goes out is handled the back sample liquid to adopt molecular weight cut-off is that the membrane ultrafiltration of 500-1000Da is handled, then with the products obtained therefrom drying, pulverize and promptly obtain temperature-resistant dendritic slow-digestion starch.
Utilize the biocatalysis transformation technology of enzyme, modify design starch molecule chain structure by multienzyme synergism hydrolysis-commentaries on classics glycosides, and acquisition content surpasses 60% temperature-resistant dendritic slow-digestion starch.
Raw starch is selected W-Gum, wheat starch, rice starch, yam starch, tapioca (flour), sweet potato starch, green starch, garbanzo starch, sorghum starch, sago starch or canna starch for use.
Temperature-resistant dendritic slow-digestion starch branch density is 10%～40%, and high temperature steaming is handled the basic no change of temperature-resistant dendritic slow-digestion starch content.
Application with described method synthetic temperature-resistant dendritic slow-digestion starch: be used for as functional supplementary material exploitation hypoglycemia heath food, energy slow-release food, nutritional factor target controlling and releasing solid support material etc.
Above-mentioned cooling, the enzyme that goes out, drying, pulverizing etc. are the current techiques of current food processing field.
The full starch analysis kit that adopts Irish Megazyme company to produce is measured the content of temperature-resistant dendritic slow-digestion starch according to the Englyst method, product SDS content 〉=60% that obtains, branch's density is 10%～40%, and high temperature steaming is handled the basic no change of back SDS content.
Branch's density of dendroid starch (branch density) represented by the ratio of α-1,6 glycosidic link in the molecular chain fine structure,
1Chemical shift is the quantity that the peak value of 5.4ppm and 5.0ppm defines α-1,4 glycosidic link and α-1,6 glycosidic link respectively on the H NMR collection of illustrative plates.
Beneficial effect of the present invention:
1, raw material of the present invention adopts the starch of cereal starch, potato starch, legume starch and other plant origin common on the domestic market, and raw material sources extensively, are not subjected to the restriction in the place of production and season.
2, be raw material with starch, adopt enzyme process synthesis technique of the present invention to handle, effectively improve the content of heatproof slow-digestion starch, guarantee the product excellent processing performance, be of high nutritive value, cost obviously descends; The present invention simultaneously adopts process for cleanly preparing, and cost is relatively low, and is pollution-free substantially to environment.
3, the present invention surpasses 60% temperature-resistant dendritic slow-digestion starch with novel starch enzymic hydrolysis-commentaries on classics glycosides effect acquisition content, good product quality, high temperature process are handled the basic no change of back SDS, and the product yield of prior art for preparing is low, poor heat stability is difficult to realize commercialization.
4, the use of the technology of the present invention has realized serialization production, and operation is simple, is easy to automatic control.
5, continuous energy release after the development product digestion of the present invention, can keep the postprandial blood sugar stable state, prevent insulin resistant, the novel modified starch that belongs to high nutritive value, can be used for developing the special-purpose fast food rod of diabetics, sportsmen's special food, functional factor target controlling and releasing coating material etc., higher scientific and technological added value is arranged, have wide potential market, have high social and economic benefit.
Specific embodiments
Embodiment 1
Taking by weighing the 1g waxy corn starch, to be made into concentration be 20% starch milk solution, handles 30min in 80 ℃ of following boilings of temperature then, makes its complete gelatinization; Treat that starch paste is cooled to room temperature, add the sodium-acetate buffer of 26mL pH 5.5 and the amylase mixed solution of 1000U/g substrate, wherein mix enzyme liquid and form by beta-amylase and transglucosidase (volume ratio 1: 1); Under 55 ℃ of conditions, constantly stir isothermal reaction 10h; The enzyme that goes out is handled the membrane ultrafiltration processing that back employing molecular weight cut-off is 650Da, then products obtained therefrom drying, pulverizing are promptly obtained temperature-resistant dendritic slow-digestion starch, SDS content 78% after measured, and branch's density is 35%, and high temperature steaming is handled the basic no change of back SDS content.
Embodiment 2
Taking by weighing the 1g yam starch, to be made into concentration be 1% starch milk solution, handles 5min in 100 ℃ of following boilings of temperature then, makes its complete gelatinization; Treat that starch paste is cooled to room temperature, add the sodium-acetate buffer of 10mL pH 4.5 and the amylase mixed solution of 500U/g substrate, wherein mix enzyme liquid and form by branch's amylase and malt-base amylase (volume ratio 1: 10); Under 35 ℃ of conditions, constantly stir isothermal reaction 24h; It is the membrane ultrafiltration processing of 500Da that the back sample liquid of handling the enzyme that goes out adopts molecular weight cut-off, then products obtained therefrom drying, pulverizing is promptly obtained temperature-resistant dendritic slow-digestion starch.SDS content 65% after measured, and temperature-resistant dendritic slow-digestion starch branch density is 19%, and high temperature steaming is handled the basic no change of temperature-resistant dendritic slow-digestion starch content.
Embodiment 3
Taking by weighing 1g long-grained nonglutinous rice starch, to be made into concentration be 40% starch milk solution, handles 15min in 90 ℃ of following boilings of temperature then, makes its complete gelatinization; Treat that starch paste is cooled to room temperature, add the sodium-acetate buffer of 50mLpH 7.0 and the amylase mixed solution of 4500U/g substrate, wherein mix enzyme liquid and form by malt-base amylase and maltose glucosyltransferase (volume ratio 1: 90); Under 90 ℃ of conditions, constantly stir isothermal reaction 1.5h; It is the membrane ultrafiltration processing of 1000Da that the back sample liquid of handling the enzyme that goes out adopts molecular weight cut-off, then products obtained therefrom drying, pulverizing is promptly obtained temperature-resistant dendritic slow-digestion starch.SDS content 71% after measured, and temperature-resistant dendritic slow-digestion starch branch density is 24%, and high temperature steaming is handled the basic no change of temperature-resistant dendritic slow-digestion starch content.
Claims (5)
1. the biosynthetic means of a temperature-resistant dendritic slow-digestion starch, it is characterized in that: be raw material with starch, utilize the biocatalysis transformation technology of beta-amylase, maltose glucosyltransferase, malt-base amylase, branch's amylase, transglucosidase, modify the reorganization starch molecular chain by multienzyme synergism hydrolysis-commentaries on classics glycosides, thereby obtain temperature-resistant dendritic slow-digestion starch; Technology is:
A) take by weighing the 1g raw starch and be made into the starch milk solution that mass concentration is 1%-50%, handle 2-30min temperature 80-100 ℃ of following boiling then, make its complete gelatinization;
B) treat that starch paste is cooled to room temperature, add the sodium-acetate buffer of 10-50mL pH 4.0-7.0 and the mixed enzyme solution of 100-5000U/g substrate, mixed enzyme solution is selected beta-amylase, maltose glucosyltransferase, malt-base amylase, branch's amylase, transglucosidase for use, optional two kinds of enzymes are formed, and the proportioning of two kinds of enzymes is volume ratio 1: 1～1: 100;
C) at temperature 30-90 ℃, pH 4.0-7.0 constantly stirs isothermal reaction 1-24h;
D) enzyme that goes out is handled the back sample liquid to adopt molecular weight cut-off is that the membrane ultrafiltration of 500-1000Da is handled, then with the products obtained therefrom drying, pulverize and promptly obtain temperature-resistant dendritic slow-digestion starch.
2. the biosynthetic means of a kind of temperature-resistant dendritic slow-digestion starch according to claim 1, it is characterized in that: the biocatalysis transformation technology that utilizes enzyme, modify design starch molecule chain structure by multienzyme synergism hydrolysis-commentaries on classics glycosides, and acquisition content surpasses 60% temperature-resistant dendritic slow-digestion starch.
3. the biosynthetic means of a kind of temperature-resistant dendritic slow-digestion starch according to claim 1 and 2, it is characterized in that: raw starch is selected W-Gum, wheat starch, rice starch, yam starch, tapioca (flour), sweet potato starch, green starch, garbanzo starch, sorghum starch, sago starch or canna starch for use.
4. the biosynthetic means of a kind of temperature-resistant dendritic slow-digestion starch according to claim 1 and 2, it is characterized in that: temperature-resistant dendritic slow-digestion starch branch density is 10%～40%, and high temperature steaming is handled the basic no change of temperature-resistant dendritic slow-digestion starch content.
5. with the application of claim 1 or 2 described method synthetic temperature-resistant dendritic slow-digestion starch, it is characterized in that: be used for as functional supplementary material exploitation hypoglycemia heath food, energy slow-release food, nutritional factor target controlling and releasing solid support material.
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Cited By (12)
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| CN106086112A (en) * | 2016-06-26 | 2016-11-09 | 陈毅忠 | A kind of method utilizing manioc waste to prepare slow-digestion starch |
| CN106497470A (en) * | 2016-11-02 | 2017-03-15 | 常州市鼎升环保科技有限公司 | A kind of preparation method of high viscosity aging resistance casting glue |
| WO2018037225A3 (en) * | 2016-08-24 | 2018-04-12 | Pepsis Limited | Enzyme compositions and diagnostics for use in enterometabolic dysfunction |
| CN108300745A (en) * | 2017-12-29 | 2018-07-20 | 齐鲁工业大学 | A kind of method that complex enzyme prepares starch special |
| CN110317843A (en) * | 2019-07-31 | 2019-10-11 | 江南大学 | A kind of supermolecule dendroid amylum grain and its synthetic method |
| CN110408665A (en) * | 2019-07-31 | 2019-11-05 | 江南大学 | A kind of Microstrip Loop dendriform starch derivatives and its processing method |
| CN110419732A (en) * | 2019-07-31 | 2019-11-08 | 江南大学 | A method of improving plant compound nutritional quality |
| CN110714040A (en) * | 2019-10-28 | 2020-01-21 | 江南大学 | Biosynthesis method of dendritic glucosyl nanoparticles |
| CN112075561A (en) * | 2020-09-30 | 2020-12-15 | 江南大学 | Preparation method of functional starch beverage |
| CN112852906A (en) * | 2021-01-13 | 2021-05-28 | 江南大学 | Method for synergistically preparing slowly digestible maltodextrin by using two starch branching enzymes |
| CN113106131A (en) * | 2021-03-31 | 2021-07-13 | 齐鲁工业大学 | Method for preparing slowly digestible starch by using composite modification method |
| CN115058466A (en) * | 2022-07-29 | 2022-09-16 | 江南大学 | Freeze-thaw stable type biological modified starch and preparation method thereof |
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| EP3909437A4 (en) * | 2019-01-10 | 2022-11-02 | Ajinomoto Co., Inc. | Method for manufacturing starch-containing food |
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Cited By (16)
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| CN106086112A (en) * | 2016-06-26 | 2016-11-09 | 陈毅忠 | A kind of method utilizing manioc waste to prepare slow-digestion starch |
| WO2018037225A3 (en) * | 2016-08-24 | 2018-04-12 | Pepsis Limited | Enzyme compositions and diagnostics for use in enterometabolic dysfunction |
| CN106497470A (en) * | 2016-11-02 | 2017-03-15 | 常州市鼎升环保科技有限公司 | A kind of preparation method of high viscosity aging resistance casting glue |
| CN108300745A (en) * | 2017-12-29 | 2018-07-20 | 齐鲁工业大学 | A kind of method that complex enzyme prepares starch special |
| CN108300745B (en) * | 2017-12-29 | 2021-04-27 | 齐鲁工业大学 | Method for preparing special modified starch by using complex enzyme |
| CN110419732B (en) * | 2019-07-31 | 2022-10-11 | 江南大学 | Method for improving nutritional quality of plant compound |
| CN110317843A (en) * | 2019-07-31 | 2019-10-11 | 江南大学 | A kind of supermolecule dendroid amylum grain and its synthetic method |
| CN110408665A (en) * | 2019-07-31 | 2019-11-05 | 江南大学 | A kind of Microstrip Loop dendriform starch derivatives and its processing method |
| CN110419732A (en) * | 2019-07-31 | 2019-11-08 | 江南大学 | A method of improving plant compound nutritional quality |
| CN110714040A (en) * | 2019-10-28 | 2020-01-21 | 江南大学 | Biosynthesis method of dendritic glucosyl nanoparticles |
| CN112075561A (en) * | 2020-09-30 | 2020-12-15 | 江南大学 | Preparation method of functional starch beverage |
| CN112075561B (en) * | 2020-09-30 | 2022-09-27 | 江南大学 | Preparation method of functional starch beverage |
| CN112852906A (en) * | 2021-01-13 | 2021-05-28 | 江南大学 | Method for synergistically preparing slowly digestible maltodextrin by using two starch branching enzymes |
| CN113106131A (en) * | 2021-03-31 | 2021-07-13 | 齐鲁工业大学 | Method for preparing slowly digestible starch by using composite modification method |
| CN115058466A (en) * | 2022-07-29 | 2022-09-16 | 江南大学 | Freeze-thaw stable type biological modified starch and preparation method thereof |
| CN115058466B (en) * | 2022-07-29 | 2023-08-25 | 江南大学 | Freeze-thawing stable biological modified starch and preparation method thereof |
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Denomination of invention: A method for biosynthesis of temperature resistant dendrimer slow digestible starch Effective date of registration: 20210308 Granted publication date: 20160504 Pledgee: Agricultural Bank of China Limited Leling City sub branch Pledgor: SHANDONG ZHONGGU STARCH SUGAR Co.,Ltd. Registration number: Y2021980001520 |
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