CN101851651B - A kind of biological synthesis method of temperature-resistant dendritic slow-digestion starch - Google Patents
A kind of biological synthesis method of temperature-resistant dendritic slow-digestion starch Download PDFInfo
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Abstract
A biological synthesis method for temperature-resistant dendritic slow-digestion starch, belongs to on-chemically modified starch technical field. The present invention utilizes the living things catalysis transformation technology of beta amylase, amylomaltase, malt-base amylase, branch's amylase, transglucosidase, by the commercialization starch of separate sources, as cornstarch, wheaten starch, rice starch, farina, tapioca, sweet potato starch, green starch, chick-pea starch, sorghum starch, sago starch and canna starch etc., modify restructuring starch molecular chain with novel hydrolysis-turn glycosides amylase, thereby obtain the temperature-resistant dendritic starch with slow digestion characteristics. The temperature-resistant dendritic slow-digestion starch that the present invention obtains can be used as functional supplementary material exploitation hypoglycemia healthy food, energy slow-release food, trophic factors target controlling and releasing carrier material etc.
Description
Technical field
What the present invention relates to is that a kind of biological enzyme of using synthesizes temperature-resistant dendritic slow-digestion starch from native starchMethod, belongs to on-chemically modified starch technical field.
Background technology
Along with the variation of social structure, quickening, eating habit and the food composition of rhythm of life change and societyAging, there is very big change in human diseases spectrum, and chronic disease, as diabetes, angiocardiopathy, fertilizerThe insulin resistance relevant disease ratios such as fat disease, hyperlipidemia, hypertension constantly rise, and have become complete at presentThe public health problem that ball is great. Prevent the pathogenetic key of above-mentioned disease to be scientific and reasonable diet structure.
Slow-digestion starch (slowlydigestiblestarch, SDS) refers to that those are digested suction completely in small intestineReceive but slow starch. According to the classification of starch bioavailability, can be defined as simulation in vitroThe starch being digested by mixed enzyme (pancreaticα-amylase, carbohydrase and invertase) in 20～120min under digestion conditionNutrition segment. As a kind of novel functional food, SDS slowly digests and assimilates after eating, sustained releaseEnergy, maintain postprandial blood sugar stable state, can prevent and treat simultaneously various and diet chronic diseases (asObesity, diabetes, glycogen storage disease and metabolic syndrome), thereby day by day become Food Science and modern nutritionA study hotspot in field. At present, the research institution of SDS mainly concentrates on American-European countries, relevant reportRoad is only confined to preparation method and physiological function research, domestic actually rare at this area research. And SDSPreparation method mainly adopts gelatinized starch recrystallization to process and forms physics state SDS, and this product is at certain processing barUnder part, easily change poor heat stability; Utilize the hydrolysis of amylase/turn glycosides effect Modified Starch moleculeThe temperature-resistant dendritic SDS of chain synthetic chemistry state, process operation and nutrition that this product can meet food keepDemand, there is wide market using value as a kind of novel functional food auxiliary material.
Summary of the invention
The object of the present invention is to provide be a kind ofly of high nutritive value, health care is good, glycemic index is lowThe biological synthesis method of temperature-resistant dendritic slow-digestion starch.
Technical scheme of the present invention: a kind of biological synthesis method of temperature-resistant dendritic slow-digestion starch, with starchFor raw material, utilize beta amylase, amylomaltase, malt-base amylase, branch's amylase,The living things catalysis transformation technology of transglucosidase, is hydrolyzed-turns glycosides by multienzyme synergism and modify restructuring starch molecular chain,Thereby acquisition temperature-resistant dendritic slow-digestion starch; Technique is:
A) take 1g raw starch and be made into the starch milk solution that mass concentration is 1%-50%, then in temperatureAt 80-100 DEG C, 2-30min is processed in boiling, makes its complete gelatinization;
B) treat that gelatinized corn starch is cooled to room temperature, add 10-50mLpH4.0-7.0 sodium-acetate buffer withThe mixed enzyme solution of 100-5000U/g substrate, mixed enzyme solution select beta amylase, amylomaltase,Malt-base amylase, branch's amylase, transglucosidase, optional two kinds of enzymes composition, the proportioning of two kinds of enzymesFor volume ratio 1: 1～1: 100;
C), at temperature 30-90 DEG C, pH4.0-7.0, constantly stirs isothermal reaction 1-24h;
D) go out enzyme process after sample liquid to adopt molecular cut off be 500-1000Da film hyperfiltration treatment, thenDry products obtained therefrom, pulverizing are obtained to temperature-resistant dendritic slow-digestion starch.
Utilize the living things catalysis transformation technology of enzyme, be hydrolyzed-turn glycosides by multienzyme synergism and modify design starchMolecular chain structure, and obtain content and exceed 60% temperature-resistant dendritic slow-digestion starch.
Raw starch select cornstarch, wheaten starch, rice starch, farina, tapioca,Sweet potato starch, green starch, chick-pea starch, sorghum starch, sago starch or canna starch.
Temperature-resistant dendritic slow-digestion starch branch density is 10%～40%, and thermophilic digestion is processed temperature-resistant dendritic slowDigestible starch content is substantially unchanged.
Application with the synthetic temperature-resistant dendritic slow-digestion starch of described method: for as functional supplementary materialExploitation hypoglycemia healthy food, energy slow-release food, trophic factors target controlling and releasing carrier material etc.
Above-mentioned cooling, the enzyme that goes out, dry, pulverizing etc. are the current techiques of current food processing field.
Adopt the full starch analysis kit that Irish Megazyme company produces to measure heatproof tree according to Englyst methodThe content of dendritic slow-digestion starch, product SDS content >=60% that obtains, branch's density is 10%～40%,And it is substantially unchanged that thermophilic digestion is processed rear SDS content.
Branch's density (branchdensity) of dendroid starch is by α-1 in strand fine structure, 6 glycosidic bondsRatio represent,1On HNMR collection of illustrative plates, chemical shift is that the peak value of 5.4ppm and 5.0ppm is fixed respectivelyJustice α-Isosorbide-5-Nitrae glycosidic bond and α-1, the quantity of 6 glycosidic bonds.
Beneficial effect of the present invention:
1, raw material of the present invention adopt cereal starch common on domestic market, potato starch, bean starch andThe starch of other plant origin, raw material sources extensively, are not subject to the restriction in the place of production and season.
2, taking starch as raw material, adopt enzyme process synthesis technique of the present invention processing, effectively improve heatproof and digest slowlyThe content of starch, ensures product excellent processing performance, is of high nutritive value, cost obviously declines; The present invention simultaneouslyAdopt process for cleanly preparing, cost is relatively low, substantially pollution-free to environment.
3, the present invention obtains content with novel starch enzyme hydrolysis-turn glycosides effect and exceedes 60% temperature-resistant dendritic slow and disappearChange starch, good product quality, it is substantially unchanged that high temperature process is processed rear SDS, and product prepared by prior artProduct yield is low, and poor heat stability is difficult to realize commercialization.
4, the use of the technology of the present invention has realized serialization production, and operation is simple, is easy to automatic control.
5, continuous energy release after the product digestion of the present invention's exploitation, can maintain postprandial blood sugar stable state, prevents from sending outRaw insulin resistance, belongs to the New Modified Starch of high nutritive value, can be used for developing diabetes patient specialFast food rod, sportsman's special food, function factor target controlling and releasing coating material etc., have higher science and technology additionalValue, has wide potential market, has higher Social benefit and economic benefit.
Specific embodiments
Embodiment 1
Taking 1g waxy corn starch, to be made into concentration be 20% starch milk solution, then at 80 DEG C of temperature, steamsBoil and process 30min, make its complete gelatinization; Treat that gelatinized corn starch is cooled to room temperature, add the acetic acid of 26mLpH5.5The novel starch enzyme mixation of sodium buffer solution and 1000U/g substrate, wherein mixes enzyme liquid by beta amylase and turns PortugalGlycosidase (volume ratio 1: 1) composition; Under 55 DEG C of conditions, constantly stir isothermal reaction 10h; The enzyme that goes out is adopted after processingThe film hyperfiltration treatment that is 650Da with molecular cut off, then obtain heatproof by dry products obtained therefrom, pulverizingDendritic slow-digestion starch, SDS content 78% after measured, branch's density is 35%, and thermophilic digestion placeAfter reason, SDS content is substantially unchanged.
Embodiment 2
Taking 1g farina, to be made into concentration be 1% starch milk solution, then boiling at 100 DEG C of temperatureProcess 5min, make its complete gelatinization; Treat that gelatinized corn starch is cooled to room temperature, add the sodium acetate of 10mLpH4.5The novel starch enzyme mixation of buffer solution and 500U/g substrate, wherein Hun Meiyeyou branch amylase and maltoseBase amylase (volume ratio 1: 10) composition; Under 35 DEG C of conditions, constantly stir isothermal reaction 24h; The enzyme place of going outAfter reason, sample liquid adopts the film hyperfiltration treatment that molecular cut off is 500Da, then by dry products obtained therefrom, powderThe broken temperature-resistant dendritic slow-digestion starch that obtains. SDS content 65% after measured, temperature-resistant dendritic slow-digestion forms sedimentPowder branch density is 19%, and it is substantially unchanged that thermophilic digestion is processed temperature-resistant dendritic slow-digestion starch content.
Embodiment 3
Taking 1g Indica Rice Starch, to be made into concentration be 40% starch milk solution, then boilery at 90 DEG C of temperatureReason 15min, makes its complete gelatinization; Treat that gelatinized corn starch is cooled to room temperature, the sodium acetate that adds 50mLpH7.0 is slowRush the novel starch enzyme mixation of liquid and 4500U/g substrate, wherein mixed enzyme liquid is by malt-base amylase and wheatBud sugar transglucosylase (volume ratio 1: 90) composition; Under 90 DEG C of conditions, constantly stir isothermal reaction 1.5h; Go outEnzyme is processed rear sample liquid and is adopted the film hyperfiltration treatment that molecular cut off is 1000Da, then products obtained therefrom is dried,Pulverize and obtain temperature-resistant dendritic slow-digestion starch. SDS content 71% after measured, temperature-resistant dendritic slow-digestionStarch branch density is 24%, and it is substantially unchanged that thermophilic digestion is processed temperature-resistant dendritic slow-digestion starch content.
Claims (2)
1. a biological synthesis method for temperature-resistant dendritic slow-digestion starch, is characterized in that: taking starch as raw material,The living things catalysis transformation technology that utilizes branch's amylase, transglucosidase, amylomaltase, passes throughMultienzyme synergism is hydrolyzed-turns glycosides and modifies restructuring starch molecular chain, thereby obtains temperature-resistant dendritic slow-digestion starch; WorkSkill is:
A) take 1g ative starch and be made into the starch milk solution that mass concentration is 1%-50%, then in temperatureAt 80-100 DEG C, 2-30min is processed in boiling, makes its complete gelatinization;
B) treat that gelatinized corn starch is cooled to room temperature, add 10-50mLpH4.0-7.0 sodium-acetate buffer withThe mixed enzyme solution of 100-5000U/g substrate, mixed enzyme solution is selected branch's amylase, transglucosidase, maltoseTransglucosylase, optional two kinds of enzymes composition, the proportioning of two kinds of enzymes is volume 1: 1～1: 100;
C), at temperature 30-90 DEG C, pH4.0-7.0, constantly stirs isothermal reaction 1-24h;
D) go out enzyme process after sample liquid to adopt molecular cut off be 500-1000Da film hyperfiltration treatment, thenDry products obtained therefrom, pulverizing are obtained to temperature-resistant dendritic slow-digestion starch.
2. the biological synthesis method of a kind of temperature-resistant dendritic slow-digestion starch according to claim 1, itsFeature is: temperature-resistant dendritic slow-digestion starch branch density is 10%～40%, and thermophilic digestion is processed heatproof branchShape slow-digestion starch content is substantially unchanged.
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| CN106086112A (en) * | 2016-06-26 | 2016-11-09 | 陈毅忠 | A kind of method utilizing manioc waste to prepare slow-digestion starch |
| GB201614415D0 (en) * | 2016-08-24 | 2016-10-05 | Pepsis Ltd | Enzymes for the treatment of human enterometabolic dysfunction |
| CN106497470A (en) * | 2016-11-02 | 2017-03-15 | 常州市鼎升环保科技有限公司 | A kind of preparation method of high viscosity aging resistance casting glue |
| CN108300745B (en) * | 2017-12-29 | 2021-04-27 | 齐鲁工业大学 | Method for preparing special modified starch by using complex enzyme |
| CN110419732B (en) * | 2019-07-31 | 2022-10-11 | 江南大学 | Method for improving nutritional quality of plant compound |
| CN110408665B (en) * | 2019-07-31 | 2021-03-26 | 江南大学 | Microstrip ring dendritic starch derivative and processing method thereof |
| CN110317843B (en) * | 2019-07-31 | 2021-03-30 | 江南大学 | Supermolecule dendritic starch particle and synthetic method thereof |
| CN110714040A (en) * | 2019-10-28 | 2020-01-21 | 江南大学 | Biosynthesis method of dendritic glucosyl nanoparticles |
| CN112075561B (en) * | 2020-09-30 | 2022-09-27 | 江南大学 | Preparation method of functional starch beverage |
| CN112852906B (en) * | 2021-01-13 | 2023-03-24 | 江南大学 | Method for synergistically preparing slowly digestible maltodextrin by using two starch branching enzymes |
| CN113106131A (en) * | 2021-03-31 | 2021-07-13 | 齐鲁工业大学 | Method for preparing slowly digestible starch by using composite modification method |
| CN115058466B (en) * | 2022-07-29 | 2023-08-25 | 江南大学 | Freeze-thawing stable biological modified starch and preparation method thereof |
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| US20080175977A1 (en) * | 2006-01-25 | 2008-07-24 | Harrison Michael D | Fiber-Containing Carbohydrate Composition |
| CN101117352A (en) * | 2007-08-29 | 2008-02-06 | 江南大学 | Method for producing high temperature stable slow-slaking amidon and uses thereof |
Non-Patent Citations (2)
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| Zihua Ao et al."Starch with a Slow Digestion Property Produced by Altering Its Chain Length, Branch Density, and Crystalline Structure".《Journal of agriculture and Food Chemistry》.2007,(第55期),第4541页左栏最后一段至右栏第一段. * |
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| EP3909437A4 (en) * | 2019-01-10 | 2022-11-02 | Ajinomoto Co., Inc. | Method for manufacturing starch-containing food |
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Effective date of registration: 20191225 Address after: 253600 Leling economic and Technological Development Zone, Shandong, Dezhou Patentee after: Shandong Zhonggu Starch Sugar Co., Ltd. Address before: 214122 Jiangsu Province, Wuxi City Lake Road No. 1800, Jiangnan University State Key Laboratory of food science and technology Patentee before: Jiangnan University |
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Denomination of invention: A method for biosynthesis of temperature resistant dendrimer slow digestible starch Effective date of registration: 20210308 Granted publication date: 20160504 Pledgee: Agricultural Bank of China Limited Leling City sub branch Pledgor: SHANDONG ZHONGGU STARCH SUGAR Co.,Ltd. Registration number: Y2021980001520 |
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