CN110419732B - Method for improving nutritional quality of plant compound - Google Patents
Method for improving nutritional quality of plant compound Download PDFInfo
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- CN110419732B CN110419732B CN201910699411.7A CN201910699411A CN110419732B CN 110419732 B CN110419732 B CN 110419732B CN 201910699411 A CN201910699411 A CN 201910699411A CN 110419732 B CN110419732 B CN 110419732B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
Abstract
The invention discloses a method for improving the nutritional quality of a plant compound, and belongs to the technical field of modern nutritional food processing. The invention takes starch with a specific cyclized dendritic structure as a carrier, and prepares the phytochemical-cyclized dendritic starch clathrate compound by a physical field-assisted solid state dispersion technology, thereby improving the nutritional quality of the fat-soluble functional factor. The product of the invention solves the problems of low solubility, low bioavailability and the like of natural plant compounds, and the application range of the product can relate to the fields of functional foods, medicines, cosmetics and the like.
Description
Technical Field
The invention belongs to the technical field of modern nutritional food processing, and particularly relates to a method for improving the nutritional quality of a plant compound.
Background
In recent years, china faces a new and severe situation: large population base, coexistence of nutrient deficiency and nutrient structure imbalance, wider disease spectrum, coexistence of disease spectrum of developing countries and developed countries; the number of non-infectious chronic diseases is increased sharply due to unreasonable dietary structure, and patients with diabetes and diabetes at the early stage have 2.5 hundred million people, patients with hyperlipidemia have 9000 ten thousand people, and patients with obesity have more than 7000 ten thousand people; when the method is advanced to an aging society, 2.41 million people are aged at 60 years old and above, and the problems of health care and geriatric diseases of the aged are getting worse. Chinese outline of food and nutrition development (2014-2020), proposes to develop nutritional health food with emphasis, control the growth of nutritional diseases, and construct a "big health" pattern. Therefore, the development of functional food has important effects on improving the physical health level of people and saving medical expense.
Plant compounds are a number of low molecular weight end products produced by plant metabolism, which protect the body against a variety of diseases and are known as "gifts given to humans by plants". The plant compounds are mainly classified into carotenoids, phenolic compounds, phytosterols, protease inhibitors, terpenes, sulfides, phytic acid, and the like, according to chemical structure or biological action. However, many natural active ingredients have the characteristics of high melting point, poor water solubility, easy oxidative decomposition, difficult digestion and absorption by human bodies and the like, so that an effective active ingredient delivery system is designed to improve the utilization rate of bioactive substances, and the like, and thus, the design has urgent needs in the field. At present, natural nutrients are developed by taking cyclodextrin, protein and lipid as matrix carriers to construct phytochemical microcapsules and emulsion products, but the existing microcapsule products taking cyclodextrin as wall materials can improve the stability of core materials, but the problems of low solubility and low bioavailability in a water phase are not solved; the emulsion taking protein as an emulsifier is sensitive to the environment, is easy to aggregate, flocculate and precipitate under equal electric points or high ionic strength, causes system instability, and is not suitable for some food systems; lipid carriers and the like are susceptible to pH or enzymes in the gastrointestinal tract environment, and are structurally damaged and dislocated to release active components, so that targeted cell absorption cannot be enhanced, and the process is complex. For the reasons mentioned above, it would be a great prospect to provide a method for improving the nutritional quality of natural plant compounds.
Disclosure of Invention
In order to solve the above problems, the present inventors have developed a method for improving the nutritional quality of a phytochemical. The product of the invention solves the problems of low solubility of natural plant compounds and the like, and the application range of the product can relate to the fields of functional foods, medicines, cosmetics and the like.
The invention is realized by the following technical scheme: the novel cyclized dendritic starch is used as a carrier, and the phytochemical-dendritic starch inclusion compound is prepared by a physical field assisted solid dispersion technology, so that the nutritional quality of the fat-soluble functional factor is improved.
A first object of the present invention is to provide a method for improving the nutritional quality of a phytochemical, said method comprising the steps of:
(1) Preparation of cyclized dendritic starch: dispersing the degreased starch in a solvent to obtain 1g/mL-5g/mL starch suspension, and adding an acid catalyst to perform starch degradation reaction; after degradation, dissolving the obtained starch degradation product in a buffer solution, and adding a transglycosidase preparation; the transglycosidase preparation comprises: a microbial source transglycosidase preparation obtained by carrying out activation culture and fermentation on archaea or bacteria to produce enzyme, or a plant source transglycosidase preparation obtained by extracting cereal grain endosperm; the sugar chain branching activity/depolymerization activity of the transglycosidase preparation is >30;
(2) Dispersing the cyclized dendritic starch in the step (1) and preparing 0.5-10mg/mL cyclized dendritic starch solution in water, and then adding a plant compound and uniformly mixing to obtain a plant compound-cyclized dendritic starch clathrate compound; wherein the mass ratio of the cyclized dendritic starch to the plant compound is (1-10): (0.5-10).
In one embodiment of the present invention, the sugar chain branching activity of the enzyme preparation refers to the activity of reducing the absorbance of linear starch-iodine complex at 660nm, and is based on the ability of the transglycosidase preparation to cleave the alpha-1, 4 glycosidic bond and transfer to another glucose residue to form a cyclic chain structure to reduce linear starch fragments, as tested in detail: branching activity (U/mL) = [ (absorbance of linear starch-iodine complex at 660 nm-absorbance of linear starch-iodine complex at 660nm after enzyme preparation addition)/(absorbance of linear starch-iodine complex at 660 nm) ] x 100/10 × 20. The sugar chain depolymerization activity means: the activity of reducing the molecular weight of the starch is the enzyme quantity required for reducing the molecular weight of the starch to 500000Da when the transglycosidase preparation acts on 1g of starch substrate and reacts for 8 hours under the optimal temperature and pH conditions for catalysis of the carbohydrase. The specific test method comprises the following steps: molecular weight reduction activity (U/mL) =1/[ (amount of enzyme required to reduce molecular weight of starch to 500000 Da/1000) × (1000 mg/sample mass) ]. Wherein the activities are all measured at 70 ℃ and pH 7.0.
In one embodiment of the present invention, the step (2) is to disperse the cyclized dendritic starch and the plant compound in water to prepare a cyclized dendritic starch solution and a plant compound solution, respectively, and then mix them.
In one embodiment of the invention, the concentration of the phytochemical solution is 0.2-0.5mg/mL.
In one embodiment of the invention, the mass ratio of the phytochemical solution to the cyclized dendritic starch solution is 5.
In one embodiment of the present invention, the defatted starch in step (1) refers to any one of corn starch, tapioca starch, potato starch, rice starch and wheat starch, or any one of common starch and waxy starch.
In one embodiment of the present invention, the degreasing treatment includes extracting a lipid component in starch with an organic solvent such as ethanol or cyclohexane.
In one embodiment of the present invention, the pH of the acid catalyst in the step (1) is 2.5 to 4.0.
In one embodiment of the present invention, the acid catalyst in step (1) comprises any one or more of phosphoric acid, boric acid, organic sulfonic acid, hydrochloride and sulfate.
In one embodiment of the invention, the glycosidase preparation has a sugar chain branching/depolymerizing activity >30. The transglycosidase preparation can be derived from any one or more of archaea, bacteria and plants.
In one embodiment of the present invention, the method for activated culture of archaea or bacteria comprises the following steps: under the aseptic condition, taking the bacterial liquid stored in the glycerin pipe, and inoculating the bacterial liquid into a sterilized seed LB culture medium for culture; the fermentation enzyme production package comprises the following steps: and after activation, inoculating the cells into a fermentation LB culture medium, culturing the cells by a constant-temperature shaking table until the cell concentration OD600 is not larger than 0.6, centrifuging the cells for 15min at 10000rpm, removing supernatant, collecting the cells, freezing, drying and crushing the cells, and the like to obtain the enzyme preparation.
In one embodiment of the invention, the microbial source comprises: bacillus stearothermophilus ATCC 7953, thermus thermophilus Caldicoloyamamurae UTM801CGMCC 6185, thermus thermophilus Streptococcus thermophilus ATCC 14485, thermus thermophilus ATCC33923, and Thermus thermophilus Aeropyrumpernix K1 (available from Japan Industrial technology institute).
In one embodiment of the present invention, the preparation of the plant-derived transglycosidase preparation comprises the following steps: weighing grain seeds in the grouting period, adding a buffer solution, homogenizing, filtering, centrifuging to obtain a crude enzyme solution, separating and purifying by an ion exchange column and gel chromatography, collecting active ingredients, and performing freeze-drying treatment to obtain the enzyme preparation.
In one embodiment of the present invention, the method for preparing the cyclized dendritic starch in the step (1) specifically comprises the following steps:
(1) Weighing 10-25g of starch subjected to degreasing treatment, suspending the starch in 6-10mL of absolute ethyl alcohol, continuously adding 10-100mL of acid catalyst solution, reacting at 20-60 ℃ for 30-120min, neutralizing the pH value after the reaction is finished, carrying out fractional precipitation, washing and drying;
(2) Dissolving starch degradation products in 50-100mL phosphate buffer solution (pH 7.0) to prepare solution with the mass concentration of 2% -30%, heating in 70 ℃ water bath for 30-60min, then adding 600-1000U transglycosidase preparation, and reacting for 8-16h under heat preservation;
(3) Heating to inactivate enzyme, centrifuging, and vacuum drying the obtained supernatant to obtain the target product.
In one embodiment of the present invention, the step (2) is mixing the vegetable compound and the cyclized dendritic starch at an environment of 30-50 ℃.
In one embodiment of the invention, the mixing is carried out at a speed of 1000 to 4000rpm for 0.5 to 5 hours.
In one embodiment of the present invention, after the mixing in the step (2), the mixture may be homogenized at 10000-15000rpm for 1-5min.
In one embodiment of the invention, the homogenized mixture may be further treated in ultrasound at a power of 200-500W for 2-20min at 0-10 deg.C.
In one embodiment of the present invention, the step (2) further comprises: and after uniformly mixing, centrifuging the mixed system, taking supernatant, and drying to obtain the plant compound-cyclic dendritic starch clathrate compound.
In one embodiment of the present invention, the method specifically includes the following steps:
(1) Every 10-25g of degreased starch is suspended in 6-10mL of absolute ethyl alcohol, 10-100mL of acid catalyst solution is continuously added to react for 30-120min at 20-60 ℃, and after the reaction is finished, the pH value is neutralized, and fractional precipitation, washing and drying are carried out; dissolving starch degradation products in 50-100mL phosphate buffer solution (pH 7.0) to prepare solution with the mass concentration of 2% -30%, heating in 70 ℃ water bath for 30-60min, then adding 600-1000U of novel transglycosidase preparation, and reacting for 8-16h under heat preservation; heating to inactivate enzyme, centrifuging, and vacuum drying the obtained supernatant to obtain cyclized dendritic starch;
(2) Weighing a certain mass of cyclized dendritic starch and a natural plant compound, wherein the former is dissolved in purified water to prepare the concentration of 0.5-10mg/mL by mass percent, and the latter is dissolved in absolute ethyl alcohol to prepare the concentration of 0.2-0.5mg/mL by mass percent.
(3) Adding the guest plant compound solution into the main body cyclized dendritic starch solution according to the proportion of 5.
(4) Placing in ultrasonic action device, controlling power at 200-500W, and treating at 0-10 deg.C for 2-20min.
(5) Centrifuging, and vacuum drying the obtained supernatant to obtain high nutritional quality plant compound-cyclic dendritic starch clathrate.
In one embodiment of the invention, the cyclized dendritic starch has a molecular weight of 3000-7000Da, the cyclized structure consists of 6.5% of alpha-1, 6 glucosidic bonds and 93.5% of alpha-1, 4 glucosidic bonds, and has an average size DP21.
In one embodiment of the present invention, the phytochemical comprises one or more fat-soluble functional factors of natural products such as curcumin, quercetin, beta-carotene, lutein, lycopene, capsaicin, resveratrol, phytosterols, and the like.
The second purpose of the invention is to provide a plant compound-cyclized dendritic starch clathrate compound by utilizing the method.
The third purpose of the invention is to apply the plant compound-cyclized dendritic starch clathrate compound in the fields of functional food, medicines and cosmetics.
The invention has the beneficial effects that:
1) The method of the invention obviously improves the solubility and the carrying capacity of the natural phytochemicals, and greatly improves the in vivo absorption and metabolism of the phytochemicals, thereby improving the bioavailability thereof and really meeting the health requirements of the consumers on modern functional foods. The grain diameter of the phytochemical-cyclized dendritic starch clathrate compound is 100-1000nm, and the phytochemical load rate is more than 1.3%; the solubility in water is improved by 10-250 times, caCO 2 The permeability of cell membrane is improved by more than 3 times.
2) The method has simple and convenient steps and controllable reaction conditions, realizes continuous, low-cost and green production, fully utilizes natural phytochemicals rich in resources in China, designs a starch-based stable carrying system for targeted transportation and activity protection of fat-soluble active factors, improves the nutritional quality of modern food, and realizes the manufacture of personalized and nutritional precise food.
3) The product prepared by the method has the effects of preventing and treating diseases and maintaining human health, can be directly put into the market as a terminal product for consumers to eat, can also be used as a raw material of food, medicine and daily chemicals, has higher scientific and technological additional value, has wide potential market, can greatly improve the value of agricultural and sideline products, has important significance for improving the health level of people, and has higher social benefit and economic benefit.
Drawings
FIG. 1 is a schematic diagram of the structure of a cyclized dendritic starch;
FIG. 2 is a MALDI-TOF-MS mass spectrum of the cyclized dendritic starch;
FIG. 3 is the particle size distribution of the β -carotene-cyclized dendritic starch clathrate in example 1.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples.
And (3) particle size determination: the sample to be tested is prepared into 0.1% (w/v) solution, and the particle size distribution is measured by a Malvern Nano ZS tester at 25 ℃.
Solubility determination method: 20mg of the inclusion compound is accurately weighed and dissolved in 1mL of deionized water, the mixture is balanced for 12 hours at room temperature in a dark place, and insoluble substances are removed by centrifugation (3000rpm, 5min) at 4 ℃. Adding 4 times volume of anhydrous ethanol into 0.2mL of centrifugate, vortex-shaking for 15min, and centrifuging (10000rpm, 5min) to separate and extract phytochemical components and starch. And (4) taking the supernatant, measuring a light absorption value by an ultraviolet spectrometer, and substituting the measured light absorption value into a standard curve equation to calculate the solubility.
The load factor calculation method comprises the following steps: referring to the content (W) of the soluble phytochemical and the mass (M) of the soluble starch obtained by the solubility measurement method, the load ratio calculation formula is as follows: load factor (%) = W/M × 100.
CaCO 2 Cell membrane permeability measurement method: cell membrane permeability is the mass percentage of phytochemicals within the Caco-2 cells and the basal portion of the lower layer of the isolation chamber to the phytochemicals initially added to the upper layer of the cells, and trans-epithelial resistance values inside and outside the culture chamber of the cell monolayer are measured using a Millicell-ERS electron voltmeter to monitor the degree of tightness between epithelial cells and determine the integrity of the cell monolayer.
Preparation of transglycosidase preparation:
plant-derived transglycosidase preparation: the enzyme preparation is obtained by extracting endosperm of grain kernels in a growth phase, weighing 100g of grain kernels in a filling phase, adding 300mL of phosphate buffer (pH 7.2, 50 mM), homogenizing, filtering, centrifuging to obtain a crude enzyme solution, separating and purifying by an ion exchange column and gel chromatography, collecting active ingredients, and performing freeze-drying treatment to obtain the enzyme preparation. Wherein the plant grain kernel endosperm comprises: rice grain endosperm, wheat grain endosperm, corn grain endosperm, sorghum grain endosperm, and the like.
Microbial transglycosidase preparation: the method comprises the steps of screening archaea or bacteria from nature, activating, culturing, fermenting and producing enzyme, wherein the strain is activated: under aseptic conditions, 200. Mu.L of the suspension stored in Glycine max tube was inoculated into a sterilized 250mL Erlenmeyer flask containing 100mL of LB medium as a seed, and cultured at 37 ℃ for 12 hours. Fermentation culture: the resulting mixture was inoculated into a 250mL Erlenmeyer flask containing 100mL of fermentation LB medium at an inoculum size of 2% (v/v) under aseptic conditions. After inoculation, the mixture is placed in a constant temperature shaking table at 37 ℃ for cultivation until the thallus concentration OD600=0.6, centrifuged for 15min at 10000rpm, the supernatant is discarded, the thallus is collected, and the enzyme preparation is obtained by the steps of freeze drying, crushing and the like. The microorganisms include: bacillus stearothermophilus ATCC 7953, thermus thermophilus Caldicoloyamamurae UTM801CGMCC 6185, thermus thermophilus Streptococcus thermophilus ATCC 14485, thermus thermophilus ATCC33923, thermus thermophilus Aeropyrumpernix K1 (available from Japan Industrial technology institute)
Example 1
Dispersing the degreased corn starch in a solvent to obtain 1g/mL starch suspension, and adding an acid catalyst to carry out starch degradation reaction; after completion of the degradation, the resulting starch degradation product was dissolved in a buffer, and added with an archaebacteria-derived transglycosidase preparation (sugar chain branching activity/depolymerization activity = 41) to react for 10 hours to obtain a cyclized dendritic starch having an average molecular weight of 4500Da in which the cyclic structure is composed of 6.5% of α -1,6 glycosidic bonds and 93.5% of α -1,4 glycosidic bonds and an average size of DP21.
Dissolving the obtained starch sample in water to prepare a cyclized dendritic starch solution with the mass percentage concentration of 0.5mg/mL; dissolving beta-carotene in absolute ethyl alcohol to prepare a beta-carotene solution with the mass percentage concentration of 0.2 mg/mL; adding a beta-carotene solution into a cyclized dendritic starch solution according to a mass ratio of 5; placing in an ultrasonic action device, controlling power at 200W, and treating at 0 deg.C for 12min; centrifuging, and vacuum drying the obtained supernatant to obtain the beta-carotene-cyclized dendritic starch clathrate compound with high nutritional quality.
The average grain diameter of the obtained inclusion compound is 280nm, and the beta-carotene loading rate is 1.4%; the solubility in water is improved by 120 times compared with that of the pure beta-carotene, caCO 2 The cell membrane permeability is improved by 4.2 times compared with that of pure beta-carotene.
Example 2
Dispersing degreased waxy rice starch in a solvent to obtain 5g/mL starch suspension, and adding an acid catalyst to carry out starch degradation reaction; after the completion of degradation, the resulting starch degradation product was dissolved in a buffer, and a plant-derived transglycosidase preparation (sugar chain branching activity/depolymerization activity = 32.1) was added and reacted for 8 hours to obtain a cyclized dendritic starch having an average molecular weight of 3750Da in which the cyclic structure is composed of 6.5% of α -1,6 glycosidic bonds and 93.5% of α -1,4 glycosidic bonds and has an average size DP21.
Dissolving the obtained starch sample in purified water to prepare the starch sample with the mass percent concentration of 10mg/mL, and dissolving curcumin in absolute ethyl alcohol to prepare the starch sample with the mass percent concentration of 0.5mg/mL; adding the curcumin solution into the main body cyclized dendritic starch solution according to the proportion of 10; placing in an ultrasonic action device, controlling power at 220W, and treating at 10 deg.C for 8min; centrifuging, and vacuum drying the obtained supernatant to obtain curcumin-cyclized dendritic starch clathrate with high nutritional quality. The average grain diameter is 164nm, and the curcumin loading rate is 2.2%; the solubility in water is improved by 80 times, caCO 2 The permeability of cell membrane is increased by 3.5 times.
Example 3
Dispersing degreased potato starch in a solvent to obtain 2g/mL starch suspension, and adding an acid catalyst to carry out starch degradation reaction; after completion of degradation, the resulting starch degradation product was dissolved in a buffer, and added with a bacterial-derived transglycosidase preparation (sugar chain branching activity/depolymerization activity = 55.0) to react for 12 hours to obtain a cyclized dendritic starch having an average molecular weight of 6700Da in which the cyclic structure is composed of 6.5% of α -1,6 glycosidic bonds and 93.5% of α -1,4 glycosidic bonds and an average size of DP21.
Dissolving a starch sample in purified water to prepare 5mg/mL of mass percent concentration, and dissolving lycopene in absolute ethyl alcohol to prepare 0.3mg/mL of mass percent concentration; adding a lycopene solution into a main body cyclized dendritic starch solution according to a ratio of 20; placing in an ultrasonic wave action device, controlling power at 250W, and treating at 4 deg.C for 2min; centrifuging, and vacuum drying the supernatant to obtain lycopene with high nutritional quality-cyclodendritic starch inclusion compounds. The average grain diameter is 455nm, and the loading rate of lycopene is 1.7%; the solubility in water is improved by 110 times, caCO 2 The permeability of the cell membrane is improved by 5.2 times.
Example 4 Process optimization
Referring to example 3, the concentration of the cyclized dendritic starch solution was changed from 5mg/mL to 0.1mg/mL and 15mg/mL, and the corresponding amounts of the solutions were adjusted to ensure the same mass ratio of the cyclized dendritic starch to lycopene and the other conditions were unchanged, and the corresponding product was prepared. The results of the obtained product are shown in table 1.
TABLE 1 results of products prepared from solutions of different concentrations of cyclized dendritic starch
Comparative example 1:
referring to example 3, the corresponding product was prepared by replacing the cyclized dendritic starch with β -cyclodextrin under otherwise unchanged conditions.
The solubility of the obtained product in water is improved by 1.1 times, caCO 2 The permeability of cell membrane is improved by 0.7 times.
The specific embodiments described herein are merely illustrative of the spirit and some of the experiments performed. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or scope of the invention as defined in the appended claims.
Claims (3)
1. A method for improving the nutritional quality of a phytochemical, said method comprising the steps of:
(1) Preparation of the cyclized dendritic starch: dispersing degreased starch in a solvent to obtain 1-5 g/mL starch suspension, and adding an acid catalyst to perform starch degradation reaction; after degradation, dissolving the obtained starch degradation product in a buffer solution, adding a transglycosidase preparation for reaction, heating to inactivate the enzyme, centrifuging, and drying the obtained supernatant in vacuum to obtain the cyclized dendritic starch;
the transglycosidase preparation is as follows: the microbial transglycosidase preparation obtained by carrying out activated culture on archaea or bacteria and producing enzyme by fermentation or the plant transglycosidase preparation obtained by extracting endosperm of cereal grains; the ratio of the branching activity and the depolymerization activity of the sugar chain of the transglycosidase preparation is more than 30; the preparation method of the microbial transglycosidase preparation comprises the following steps: under the aseptic condition, taking a bacterial solution stored in the glycerinum tube, and inoculating the bacterial solution into a sterilized seed LB culture medium for culture; the fermentation enzyme production package comprises the following steps: activating, inoculating in fermentation LB culture medium, and culturing with constant temperature shaking table to obtain thallus concentration OD 600 Centrifuging at 10000rpm for 15min for 0.6, collecting the mycelia by discarding the supernatant, and pulverizing the lyophilized powder to obtain enzyme preparation; the microorganism source is as follows: bacillus stearothermophilusBacillus stearothermophilusATCC 7953 Thermus extremophilusCalditerricolayamamuraeUTM801CGMCC 6185, thermococcus extremusStreptococcus thermophilusATCC 14485 Thermus thermophilusThermus thermophilesATCC33923, archaebacterium thermophilum K1; the preparation method of the plant source transglycosidase preparation comprises the following steps: weighing grain seeds in the grouting period, adding a buffer solution, homogenizing, filtering, centrifuging to obtain a crude enzyme solution, separating and purifying by an ion exchange column and gel chromatography, collecting active ingredients, and performing freeze-drying treatment to obtain an enzyme preparation;
(2) Dispersing the cyclized dendritic starch in the step (1) in water to prepare a 0.5-10mg/mL cyclized dendritic starch solution, then adding the plant compound solution, and uniformly mixing to obtain a plant compound-cyclized dendritic starch clathrate compound; wherein the mass ratio of the cyclized dendritic starch solution to the plant compound solution is 5; the plant compound is one or more fat-soluble functional factors selected from curcumin, quercetin, beta-carotene, lutein, lycopene, capsaicin, resveratrol and phytosterol; the concentration of the plant compound solution is 0.2-0.5 mg/mL; the phytochemical solution is a solution obtained by dispersing the phytochemical in ethanol.
2. A phytochemical-cyclized dendritic starch clathrate compound prepared by the method of claim 1.
3. The use of the plant compound-cyclic dendritic starch clathrate of claim 2 in the field of functional food, pharmaceutical, and cosmetic preparation.
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