CN101827934B - D4去饱和酶和d5延伸酶 - Google Patents

D4去饱和酶和d5延伸酶 Download PDF

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Publication number
CN101827934B
CN101827934B CN200780100540.5A CN200780100540A CN101827934B CN 101827934 B CN101827934 B CN 101827934B CN 200780100540 A CN200780100540 A CN 200780100540A CN 101827934 B CN101827934 B CN 101827934B
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CN200780100540.5A
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English (en)
Chinese (zh)
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CN101827934A (zh
Inventor
A·M·伯杰
G·S·吉鲁阿德
H·瑞迪亚宁塔斯
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DSM NUTRITIONAL Co Ltd
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Ocean Nutrition Canada Ltd
DSM Nutritional Products AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200780100540.5A 2007-07-13 2007-10-31 D4去饱和酶和d5延伸酶 Expired - Fee Related CN101827934B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94973007P 2007-07-13 2007-07-13
US60/949,730 2007-07-13
PCT/IB2007/004553 WO2009010825A2 (en) 2007-07-13 2007-10-31 D4 desaturases and d5 elongases

Related Child Applications (1)

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CN201210579165.XA Division CN103074312B (zh) 2007-07-13 2007-10-31 D4去饱和酶和d5延伸酶

Publications (2)

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CN101827934A CN101827934A (zh) 2010-09-08
CN101827934B true CN101827934B (zh) 2014-02-26

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Family Applications (2)

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CN200780100540.5A Expired - Fee Related CN101827934B (zh) 2007-07-13 2007-10-31 D4去饱和酶和d5延伸酶
CN201210579165.XA Expired - Fee Related CN103074312B (zh) 2007-07-13 2007-10-31 D4去饱和酶和d5延伸酶

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US (2) US8765422B2 (enExample)
EP (1) EP2171049A4 (enExample)
JP (1) JP2010533003A (enExample)
CN (2) CN101827934B (enExample)
AU (1) AU2007356650A1 (enExample)
CA (1) CA2695161C (enExample)
MX (1) MX2010000548A (enExample)
WO (1) WO2009010825A2 (enExample)
ZA (1) ZA201001045B (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010000548A (es) * 2007-07-13 2010-03-09 Ocean Nutrition Canada Ltd Desaturasas d4 y elongasas d5.
US9133463B2 (en) 2011-03-07 2015-09-15 Dsm Nutritional Products Ag Engineering microorganisms
CN102559710B (zh) * 2011-06-27 2013-10-30 中国农业科学院油料作物研究所 等鞭金藻δ4-脂肪酸去饱和酶基因及其克隆方法
AU2014229307B2 (en) * 2013-03-13 2017-08-31 Dsm Nutritional Products Ag Engineering microorganisms

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002026946A2 (en) * 2000-09-28 2002-04-04 Bioriginal Food & Science Corporation Fad4, fad5, fad5-2, and fad6, fatty acid desaturase family members and uses thereof
WO2005118814A2 (en) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070970B2 (en) 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
PT1254238E (pt) * 2000-02-09 2009-11-20 Basf Se Novo gene de elongase e processo para a preparação de ácidos gordos poli-insaturados
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
ATE495263T1 (de) 2002-03-16 2011-01-15 Univ York Transgene pflanzen, die fettsäurebiosynthese enzyme exprimieren
CN1860211B (zh) * 2003-08-01 2010-08-04 巴斯福植物科学有限公司 用于在转基因生物中产生多不饱和脂肪酸的方法
PL1723220T3 (pl) 2004-02-27 2013-09-30 Basf Plant Science Gmbh Sposób wytwarzania wielokrotnie nienasyconych kwasów tłuszczowych w roślinach transgenicznych
JP4567047B2 (ja) * 2004-02-27 2010-10-20 ビーエーエスエフ プラント サイエンス ゲーエムベーハー トランスジェニック植物における多価不飽和脂肪酸の製造方法
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
JP5886511B2 (ja) 2005-06-07 2016-03-16 ディーエスエム ニュートリショナル プロダクツ アーゲーＤＳＭ Ｎｕｔｒｉｔｉｏｎａｌ Ｐｒｏｄｕｃｔｓ ＡＧ 脂質および抗酸化剤の製造のための真核微生物
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
MX2010000548A (es) * 2007-07-13 2010-03-09 Ocean Nutrition Canada Ltd Desaturasas d4 y elongasas d5.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002026946A2 (en) * 2000-09-28 2002-04-04 Bioriginal Food & Science Corporation Fad4, fad5, fad5-2, and fad6, fatty acid desaturase family members and uses thereof
WO2005118814A2 (en) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Burja AM 等.Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species: screening of strains and optimization of omega-3 production..《Appl Microbiol Biotechnol.》.2006,第72卷(第6期),第1161-1169页. *
Xiao Qiu 等.Identification of a Δ4 Fatty Acid Desaturase fromThraustochytrium sp. Involved in the Biosynthesis of Docosahexanoic Acid by Heterologous Expression inSaccharomyces cerevisiae and Brassica juncea.《THE JOURNAL OF BIOLOGICAL CHEMISTRY》.2001,第276卷(第34期),第31561–31566页. *

Also Published As

Publication number Publication date
AU2007356650A1 (en) 2009-01-22
EP2171049A4 (en) 2010-12-29
US20140329291A1 (en) 2014-11-06
CA2695161C (en) 2018-05-22
ZA201001045B (en) 2011-06-29
US20110059494A1 (en) 2011-03-10
JP2010533003A (ja) 2010-10-21
WO2009010825A3 (en) 2010-05-27
US9441212B2 (en) 2016-09-13
CA2695161A1 (en) 2009-01-22
CN103074312A (zh) 2013-05-01
HK1148309A1 (en) 2011-09-02
MX2010000548A (es) 2010-03-09
EP2171049A2 (en) 2010-04-07
US8765422B2 (en) 2014-07-01
CN101827934A (zh) 2010-09-08
CN103074312B (zh) 2015-01-07
WO2009010825A2 (en) 2009-01-22

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