CA2695161C - D4 desaturases and d5 elongases - Google Patents

D4 desaturases and d5 elongases Download PDF

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Publication number
CA2695161C
CA2695161C CA2695161A CA2695161A CA2695161C CA 2695161 C CA2695161 C CA 2695161C CA 2695161 A CA2695161 A CA 2695161A CA 2695161 A CA2695161 A CA 2695161A CA 2695161 C CA2695161 C CA 2695161C
Authority
CA
Canada
Prior art keywords
disclosed
desaturase
sequence
cell
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2695161A
Other languages
English (en)
French (fr)
Other versions
CA2695161A1 (en
Inventor
Adam M. Burja
Gabrielle S. Girouard
Helia Radianingtyas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM Nutritional Products AG
Original Assignee
DSM Nutritional Products AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM Nutritional Products AG filed Critical DSM Nutritional Products AG
Publication of CA2695161A1 publication Critical patent/CA2695161A1/en
Application granted granted Critical
Publication of CA2695161C publication Critical patent/CA2695161C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2695161A 2007-07-13 2007-10-31 D4 desaturases and d5 elongases Expired - Fee Related CA2695161C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94973007P 2007-07-13 2007-07-13
US60/949,730 2007-07-13
PCT/IB2007/004553 WO2009010825A2 (en) 2007-07-13 2007-10-31 D4 desaturases and d5 elongases

Publications (2)

Publication Number Publication Date
CA2695161A1 CA2695161A1 (en) 2009-01-22
CA2695161C true CA2695161C (en) 2018-05-22

Family

ID=40260143

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2695161A Expired - Fee Related CA2695161C (en) 2007-07-13 2007-10-31 D4 desaturases and d5 elongases

Country Status (9)

Country Link
US (2) US8765422B2 (enExample)
EP (1) EP2171049A4 (enExample)
JP (1) JP2010533003A (enExample)
CN (2) CN103074312B (enExample)
AU (1) AU2007356650A1 (enExample)
CA (1) CA2695161C (enExample)
MX (1) MX2010000548A (enExample)
WO (1) WO2009010825A2 (enExample)
ZA (1) ZA201001045B (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8765422B2 (en) * 2007-07-13 2014-07-01 Dsm Nutritional Products Ag D4 desaturases and D5 elongases
CA2829296C (en) * 2011-03-07 2019-10-29 ShuoCheng ZHANG Engineering thraustochytrid microorganisms
CN102559710B (zh) * 2011-06-27 2013-10-30 中国农业科学院油料作物研究所 等鞭金藻δ4-脂肪酸去饱和酶基因及其克隆方法
WO2014141098A1 (en) 2013-03-13 2014-09-18 Dsm Nutritional Products Ag Engineering microorganisms

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070970B2 (en) 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
ES2331228T3 (es) 2000-02-09 2009-12-28 Basf Se Nuevo gen de elongasa y proceso para la preparacion de acidos grasos poliinsaturados.
CN100567483C (zh) * 2000-09-28 2009-12-09 生物源食物及科学公司 脂肪酸去饱和酶家族成员fad4、fad5、fad5-2和fad6及它们的应用
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
US7705202B2 (en) 2002-03-16 2010-04-27 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
CA3037924A1 (en) * 2003-08-01 2005-02-10 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids in transgenic organisms
EP3543324B1 (de) 2004-02-27 2022-11-30 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigten fettsäuren in transgenen pflanzen
EP4219670A3 (de) 2004-02-27 2023-08-09 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
EP2357243B1 (en) 2004-04-22 2018-12-12 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
ES2351973T3 (es) * 2004-06-04 2011-02-14 Fluxome Sciences A/S Células metabólicamente modificadas por ingeniería para la producción de ácidos grasos poliinsaturados.
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast
EP3238543B1 (en) 2005-06-07 2019-03-20 DSM Nutritional Products AG Eukaryotic microorganisms for producing lipids and antioxidants
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
US8765422B2 (en) * 2007-07-13 2014-07-01 Dsm Nutritional Products Ag D4 desaturases and D5 elongases

Also Published As

Publication number Publication date
CN103074312A (zh) 2013-05-01
CA2695161A1 (en) 2009-01-22
AU2007356650A1 (en) 2009-01-22
CN101827934A (zh) 2010-09-08
US9441212B2 (en) 2016-09-13
MX2010000548A (es) 2010-03-09
EP2171049A4 (en) 2010-12-29
HK1148309A1 (en) 2011-09-02
CN101827934B (zh) 2014-02-26
US20110059494A1 (en) 2011-03-10
US20140329291A1 (en) 2014-11-06
CN103074312B (zh) 2015-01-07
JP2010533003A (ja) 2010-10-21
WO2009010825A3 (en) 2010-05-27
WO2009010825A2 (en) 2009-01-22
EP2171049A2 (en) 2010-04-07
US8765422B2 (en) 2014-07-01
ZA201001045B (en) 2011-06-29

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Effective date: 20201102