CN101822320B - Vitamin water aqua and preparation method thereof - Google Patents
Vitamin water aqua and preparation method thereof Download PDFInfo
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- CN101822320B CN101822320B CN2010101796369A CN201010179636A CN101822320B CN 101822320 B CN101822320 B CN 101822320B CN 2010101796369 A CN2010101796369 A CN 2010101796369A CN 201010179636 A CN201010179636 A CN 201010179636A CN 101822320 B CN101822320 B CN 101822320B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D7/00—Edible oil or fat compositions containing an aqueous phase, e.g. margarines
- A23D7/01—Other fatty acid esters, e.g. phosphatides
- A23D7/011—Compositions other than spreads
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
- A23D9/013—Other fatty acid esters, e.g. phosphatides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
- A61K31/51—Thiamines, e.g. vitamin B1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7135—Compounds containing heavy metals
- A61K31/714—Cobalamins, e.g. cyanocobalamin, i.e. vitamin B12
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
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Abstract
The invention relates to a vitamin water aqua. The raw materials of the water aqua comprise liposome, vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin H, folic acid and solubilizer. In the invention, liposoluble vitamins are coated in a microcapsule structure of the liposome and form a homogenized and stable water aqua with water-soluble vitamins. The water aqua has stable storage character in a long time, smaller grain diameter of components and a cytoid structure and can activate the self immunologic function after entering an animal body to keep the immune level of organisms in a higher state; and meanwhile, a plurality of vitamins can be effectively stored in the organism, so that the invention avoids the problems of the loss and the insufficient utilization rate of the vitamins in the metabolic process, remarkably improves the utilization rate and the conversion rate of feed, improves the antibody level of animal organisms and enhances the disease resistant capability.
Description
Technical field
The invention belongs to a kind of vitamin(e) supplement in the animal aquaculture technology, especially a kind of vitamin water aqua and preparation method thereof.
Background technology
Vitamin is that body is kept the essential a kind of organic substance of normal function institute, but it can not synthesize in vivo, or synthetic amount seldom, need be by food intake.Vitamin is being regulated the body metabolism and is being kept aspect such as physiological function and bringing into play important effect, it mainly participates in number of chemical reaction in the internal metabolism widely with the form of coenzyme and catalyst, thereby the eucaryotic cell structure of assurance body tissue organ and function is normal, keep the daily normal health of animal and comings and goings, the shortage of vitamin can cause organism metabolic disorder, influences animal health and normal biological activity.
Vitamin can be divided into by its solubility: liposoluble vitamin and water soluble vitamin.The effect of liposoluble vitamin is various, except participating in directly and influencing the special metabolic process, also is combined with intracellular receptor, influences special genes and expresses.As the term suggests water soluble vitamin is can be water-soluble, be insoluble to fat and organic solvent, eat in a large number generally can not produce and accumulate and toxic action, the overwhelming majority participates in various enzyme system work with coenzyme or prothetic group form, all plays epochmaking effect in many links of intermediate supersession.As seen liposoluble vitamin and water soluble vitamin have great effect to the health of animal health, but because liposoluble vitamin can only be dissolved in the liposoluble substance, so the utilization rate of liposoluble vitamin when reality is used is lower, especially how liposoluble vitamin and water soluble vitamin are made a kind of homogeneous, stable aqua, be the difficult problem that aquaculture remains to be captured always.At present known method exists that active ingredient in the aqua is less, aqua is unstable and the shortcoming of complex process.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of homogeneous, stable vitamin water aqua are provided, and the raw material weight of this aqua is formed and is comprised: liposome, 1.89～5.85 parts vitamin A, 2～4 parts vitamin D, 1～3 part vitamin E, 1～2 part vitamin K, 4～6 parts vitamin B1,2～4 parts vitamin B2,2～6 parts vitamin B6,1～2 part cobalamin, 1～6 part vitamin C, 2～4 parts biotin, 1～2 part folic acid and solubilizer.
The raw material weight of liposome is formed and is comprised: 0～10 part soybean lecithin, 0～15 part DSPC, 0～40 part sorbierite and 5 parts cholesterol.
Solubilizer is Macrogol 6000 and the polyvinylpyrrolidone that mixed by weight 2: 1.
Another object of the present invention provides a kind of preparation method of vitamin water aqua, and this method may further comprise the steps:
(1) preparation liposome
One or more and cholesterol in soybean lecithin, DSPC or the sorbierite are mixed the back add in the reactor, the reaction time is 30-45min, and reaction temperature is 20～65 ℃;
(2) preparation liposoluble vitamin solution
Vitamin A, vitamin D, vitamin E, vitamin K and solubilizer are dissolved in the mixed solution of organic solvent chloroform and ethanol, make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
Vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, biotin, folic acid are dissolved in the PBS buffer solution, make water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and organic solvent is removed in vacuum evaporation, adds water soluble vitamin solution again, and normal temperature left standstill 2～4 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid.
And the finished product after step (5) is handled also passes through the 0.45um filtering with microporous membrane.
And the soybean lecithin described in the step (1) is that 0～10 part, DSPC are that 0～15 part, sorbierite are that 0～40 part, cholesterol are 5 parts, more than is parts by weight.
And the solubilizer described in the step (2) is Macrogol 6000 and the polyvinylpyrrolidone that mixed by weight 2: 1.
And the volume ratio of the chloroform described in the step (2) and ethanol is 2: 1.
And the pH value of the PBS buffer solution described in the step (3) is 6.0～7.4, and the buffer solution temperature is 30～45 ℃.
And the vacuum evaporation condition described in the step (4) is that organic solvent is removed in vacuum evaporation below 50 ℃.
Advantage of the present invention and good effect are:
Utilize the microcapsule structure of liposome among the present invention, liposoluble vitamin is wrapped in inside and forms homogeneous, stable aqua with water soluble vitamin.It is stable to deposit proterties in this aqua is long-time, its grain diameter of components is less and have a structure of class cell, entering the autoimmune function that can activate body in the animal body makes the immunity of organism level keep higher state, multivitamin can effectively be stored in the body, the loss of vitamin in metabolic process and the problem of utilization rate deficiency have been avoided, obviously improved utilization rate and the conversion ratio of feed, improve animal body antibody horizontal, strengthened resistance against diseases.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
The invention provides a kind of homogeneous, stable vitamin water aqua, the raw material weight of this aqua is formed and is comprised: liposome, 1.89～5.85 parts vitamin A, 2～4 parts vitamin D, 1～3 part vitamin E, 1～2 part vitamin K, 4～6 parts vitamin B1,2～4 parts vitamin B2,2～6 parts vitamin B6,1～2 part cobalamin, 1～6 part vitamin C, 2～4 parts biotin, 1～2 part folic acid and solubilizer.
The raw material weight of liposome is formed and is comprised: 0～10 part soybean lecithin, 0～15 part DSPC, 0～40 part sorbierite and 5 parts cholesterol.Solubilizer is Macrogol 6000 and the polyvinylpyrrolidone that mixed by weight 2: 1.
Embodiment 1
A kind of preparation method of vitamin water aqua may further comprise the steps:
(1) preparation liposome
10g soybean lecithin and 5g cholesterol are mixed in the back adding reactor, and the reaction time is 30-45min, and reaction temperature is 20 ℃;
(2) preparation liposoluble vitamin solution
11000000IU (3.78g) vitamin A, 160000000IU (4g) vitamin D, 2000IU (2g) vitamin E, 1000IU (1g) vitamin K, 10g Macrogol 6000 and 5g polyvinylpyrrolidone are dissolved in the mixed solution of organic solvent chloroform and ethanol (weight ratio of chloroform and ethanol is 2: 1), make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
It is 6.0 PBS buffer solution that 4g vitamin B1,4g vitamin B2,2g vitamin B6,2g cobalamin, 4g vitamin C, 2g biotin, 2g folic acid are dissolved in the pH value, and the buffer solution temperature is 30 ℃, makes water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and at 0.08Mpa, organic solvent (chloroform and ethanol) is removed in vacuum evaporation under the condition below 50 ℃, adds water soluble vitamin solution again, and normal temperature left standstill 2 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid, and the particle diameter of the solid particle in the finished product is 0.1um～0.4um.
(6) be harmful to bacterium, tinning through the 0.45um filtering with microporous membrane to go out.
Embodiment 2
A kind of preparation method of vitamin water aqua may further comprise the steps:
(1) preparation liposome
10g soybean lecithin, 20g sorbierite and 5g cholesterol are mixed the back add in the reactor, the reaction time is 3045min, and reaction temperature is 65 ℃;
(2) preparation liposoluble vitamin solution
5500000IU (1.89g) vitamin A, 160000000IU (4g) vitamin D, 2000IU (2g) vitamin E, 1000IU (1g) vitamin K, 10g Macrogol 6000 and 5g polyvinylpyrrolidone are dissolved in the mixed solution of organic solvent chloroform and ethanol (weight ratio of chloroform and ethanol is 2: 1), make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
It is 7.4 PBS buffer solution that 4g vitamin B1,2g vitamin B2,2g vitamin B6,1g cobalamin, 6g vitamin C, 2g biotin, 2g folic acid are dissolved in the pH value, and the buffer solution temperature is 45 ℃, makes water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and at 0.08～0.1Mpa, organic solvent (chloroform and ethanol) is removed in vacuum evaporation under the condition below 50 ℃, adds water soluble vitamin solution again, and normal temperature left standstill 4 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid, and the particle diameter of the solid particle in the finished product is 0.1um～0.4um.
(6) be harmful to bacterium, tinning through the 0.45um filtering with microporous membrane to go out.
Embodiment 3
A kind of preparation method of vitamin water aqua may further comprise the steps:
(1) preparation liposome
5g soybean lecithin, 5g DSPC, 20g sorbierite and 5g cholesterol are mixed the back add in the reactor, the reaction time is 30-45min, and reaction temperature is 55 ℃;
(2) preparation liposoluble vitamin solution
11000000IU (3.78g) vitamin A, 160000000IU (4g) vitamin D, 2000IU (2g) vitamin E, 2000IU (2g) vitamin K, 10g Macrogol 6000 and 5g polyvinylpyrrolidone are dissolved in the mixed solution of organic solvent chloroform and ethanol (weight ratio of chloroform and ethanol is 2: 1), make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
It is 7.0 PBS buffer solution that 4g vitamin B1,2g vitamin B2,6g vitamin B6,2g cobalamin, 4g vitamin C, 2g biotin, 2g folic acid are dissolved in the pH value, and the buffer solution temperature is 32 ℃, makes water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and at 0.08～0.09Mpa, organic solvent (chloroform and ethanol) is removed in vacuum evaporation under the condition below 50 ℃, adds water soluble vitamin solution again, and normal temperature left standstill 3.5 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid, and the particle diameter of the solid particle in the finished product is 0.1um～0.4um.
(6) be harmful to bacterium, tinning through the 0.45um filtering with microporous membrane to go out.
Embodiment 4
A kind of preparation method of vitamin water aqua may further comprise the steps:
(1) preparation liposome
10g DSPC, 40g sorbierite and 5g cholesterol are mixed the back add in the reactor, the reaction time is 30-45min, and reaction temperature is 45 ℃;
(2) preparation liposoluble vitamin solution
11000000IU (3.78g) vitamin A, 80000000 (2g) vitamin D, 2000IU (2g) vitamin E, 2000IU (2g) vitamin K, 10g Macrogol 6000 and 5g polyvinylpyrrolidone are dissolved in the mixed solution of organic solvent chloroform and ethanol (weight ratio of chloroform and ethanol is 2: 1), make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
It is 6.5 PBS buffer solution that 4g vitamin B1,4g vitamin B2,6g vitamin B6,2g cobalamin, 1g vitamin C, 4g biotin, 1g folic acid are dissolved in the pH value, and the buffer solution temperature is 41 ℃, makes water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and at 0.09MPa, organic solvent (chloroform and ethanol) is removed in vacuum evaporation under the condition below 50 ℃, adds water soluble vitamin solution again, and normal temperature left standstill 2.5 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid, and the particle diameter of the solid particle in the finished product is 0.1um～0.4um.
(6) be harmful to bacterium, tinning through the 0.45um filtering with microporous membrane to go out.
Embodiment 5
A kind of preparation method of vitamin water aqua may further comprise the steps:
(1) preparation liposome
15g DSPC and 5g cholesterol are mixed in the back adding reactor, and the reaction time is 30-45min, and reaction temperature is 25 ℃;
(2) preparation liposoluble vitamin solution
17000000 (5.85g) vitamin A, 160000000IU (4g) vitamin D, 2000IU (2g) vitamin E, 1000IU (1g) vitamin K, 10g Macrogol 6000 and 5g polyvinylpyrrolidone are dissolved in the mixed solution of organic solvent chloroform and ethanol (weight ratio of chloroform and ethanol is 2: 1), make liposoluble vitamin solution;
(3) preparation water soluble vitamin solution
It is 6.8 PBS buffer solution that 6g vitamin B1,4g vitamin B2,6g vitamin B6,2g cobalamin, 4g vitamin C, 2g biotin, 2g folic acid are dissolved in the pH value, and the buffer solution temperature is 37 ℃, makes water soluble vitamin solution;
(4) preparation vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and at 0.08Mpa, organic solvent (chloroform and ethanol) is removed in vacuum evaporation under the condition below 50 ℃, adds water soluble vitamin solution again, and normal temperature left standstill 4 hours;
(5) the vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid, and the particle diameter of the solid particle in the finished product is 0.1um～0.4um.
(6) be harmful to bacterium, tinning through the 0.45um filtering with microporous membrane to go out.
The vitamin water aqua stability analysis of embodiment 1～5 the results are shown in Table 1, table 2 and table 3:
| Time | 1 month | 3 months | 6 months | 12 months | 18 months |
| Embodiment 1 | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous |
| Embodiment 2 | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous |
| Embodiment 3 | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous |
| Embodiment 4 | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous |
| Embodiment 5 | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous | The weak yellow liquid stable homogeneous |
Table 1: the stability analysis of aqua long-term storage
As can be known from the results of Table 1, this vitamin water aqua is after long-term storage, and proterties is stable.
| Time | 1 month (%) | 3 months (%) | 6 months (%) | 12 months (%) | 18 months (%) |
| Vitamin A | 99.97 | 99.95 | 99.95 | 99.94 | 99.93 |
| Vitamin D | 99.94 | 99.93 | 99.93 | 99.91 | 99.91 |
| Vitamin E | 99.96 | 99.95 | 99.93 | 99.93 | 99.91 |
| Vitamin K | 99.99 | 99.97 | 99.97 | 99.95 | 99.95 |
Table 2: the liposoluble constituent average content of aqua long-term storage is analyzed
As can be known from the results of Table 2, this vitamin water aqua after long-term storage, liposoluble constituent content abundance.
Table 3: use the animal sign analysis behind this vitamin water aqua
As can be known from the results of Table 3, after the animal poultry and livestock used this vitamin water aqua, its efficiency of feed utilization, weightening finish index and immune level all significantly improve, and directly use, and be easy and simple to handle.
In sum, the present invention deposits that proterties is stable after the long period, active constituent content is sufficient, obviously improve efficiency of feed utilization and conversion ratio, raising body antibody horizontal strengthens resistance against diseases, product is easy to use, solved liposoluble vitamin absorption, utilize low, awkward problem.
Claims (6)
1. a vitamin water aqua is characterized in that the weight composition of the raw material of this aqua comprises: liposome, 1.89～5.85 parts vitamin A, 2～4 parts vitamin D, 1～3 part vitamin E, 1～2 part vitamin K, 4～6 parts Cobastab
1, 2～4 parts Cobastab
2, 2～6 parts Cobastab
6, 1～2 part Cobastab
12, 1～6 part vitamin C, 2～4 parts biotin, 1～2 part folic acid and solubilizer;
The raw material weight of described liposome is formed and is comprised: 0～10 part soybean lecithin, 0～15 part DSPC, 0～40 part sorbierite and 5 parts cholesterol;
The preparation method of described vitamin water aqua may further comprise the steps:
⑴ prepare liposome
One or more and cholesterol in soybean lecithin, DSPC or the sorbierite are mixed the back add in the reactor, the reaction time is 30-45min, and reaction temperature is 20～65 ℃;
⑵ prepare liposoluble vitamin solution
Vitamin A, vitamin D, vitamin E, vitamin K and solubilizer are dissolved in the mixed solution of organic solvent chloroform and ethanol, make liposoluble vitamin solution;
⑶ prepare water soluble vitamin solution
With Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, vitamin C, biotin, folic acid be dissolved in the PBS buffer solution, makes water soluble vitamin solution;
⑷ prepare vitamin water aqua
Liposoluble vitamin solution is dissolved in the liposome, and organic solvent is removed in vacuum evaporation, adds water soluble vitamin solution again, and normal temperature left standstill 2～4 hours;
⑸ vitamin water aqua homogeneous is handled
Vitamin water aqua after leaving standstill is made finished product after with milling treatment of colloid.
2. a kind of vitamin water aqua according to claim 1 is characterized in that: Macrogol 6000 and the polyvinylpyrrolidone of described solubilizer for mixing by weight 2:1.
3. a kind of vitamin water aqua according to claim 1 is characterized in that: the finished product after described step ⑸ handles also passes through the 0.45um filtering with microporous membrane.
4. a kind of vitamin water aqua according to claim 1, it is characterized in that: the volume ratio of the chloroform described in the step ⑵ and ethanol is 2:1.
5. a kind of vitamin water aqua according to claim 1, it is characterized in that: the pH value of the PBS buffer solution described in the step ⑶ is 6.0～7.4, the buffer solution temperature is 30～45 ℃.
6. a kind of vitamin water aqua according to claim 1 is characterized in that: the vacuum evaporation condition described in the step ⑷ is that organic solvent is removed in vacuum evaporation below 50 ℃.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101796369A CN101822320B (en) | 2010-05-22 | 2010-05-22 | Vitamin water aqua and preparation method thereof |
| AU2011101750A AU2011101750A4 (en) | 2010-05-22 | 2011-05-20 | Aqueous formulation of vitamins and preparation method thereof |
| KR1020127021887A KR20130083822A (en) | 2010-05-22 | 2011-05-20 | Aqueous formulation of vitamins and preparation method thereof |
| PCT/CN2011/074437 WO2011147281A1 (en) | 2010-05-22 | 2011-05-20 | Aqueous formulation of vitamins and preparation method thereof |
| AU2011257786A AU2011257786A1 (en) | 2010-05-22 | 2011-05-20 | Aqueous formulation of vitamins and preparation method thereof |
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| CN2010101796369A CN101822320B (en) | 2010-05-22 | 2010-05-22 | Vitamin water aqua and preparation method thereof |
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| CN101822320A CN101822320A (en) | 2010-09-08 |
| CN101822320B true CN101822320B (en) | 2013-07-03 |
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| CN (1) | CN101822320B (en) |
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| CN101822320B (en) * | 2010-05-22 | 2013-07-03 | 鼎正动物药业（天津）有限公司 | Vitamin water aqua and preparation method thereof |
| CN102552058B (en) * | 2011-12-27 | 2013-05-01 | 东南大学 | Compound vitamin lipid nanoparticle and preparation method thereof |
| CN103549219B (en) * | 2013-11-19 | 2015-03-04 | 厦门星鲨动物保健品厂 | Drug feed additive for promoting skin health of aquatic animals and preparation method thereof |
| CN103783323B (en) * | 2014-02-05 | 2016-05-18 | 褚旭 | A kind of animal complex vitamin premix preparation method |
| CN104000833B (en) * | 2014-03-07 | 2016-05-18 | 江西赣南海欣药业股份有限公司 | A kind of vitamin D2With calcium levulinate injection composition and preparation method thereof |
| KR20160008275A (en) * | 2014-07-14 | 2016-01-22 | 배대승 | Vitamin C Composition with Improved Absorption Rate and the Method thereof (1) |
| CN108208411B (en) * | 2018-03-01 | 2021-08-03 | 山东德州神牛药业有限公司 | Metabolism immunoregulation composite solution for livestock and poultry and preparation method thereof |
| CN109044974A (en) * | 2018-09-06 | 2018-12-21 | 澳汀斯（广州）生物医药科技有限公司 | Coat nano vesicle preparations and its application of vitamin D and vitamin K |
| CN114766674A (en) * | 2022-04-26 | 2022-07-22 | 湖南赛菱思生物科技有限公司 | Microcapsule powder compounded with MCT and phospholipid oil as well as preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1096194A (en) * | 1993-06-07 | 1994-12-14 | 北京和德实业公司 | The cosmetics of the lecithin liposome of embedding vitamin and method for making thereof |
| CN1225815A (en) * | 1998-06-10 | 1999-08-18 | 南方医院 | Dried lipid article and preparation method therefor |
| CN1364459A (en) * | 2001-07-02 | 2002-08-21 | 刘银良 | Method for preparing slow-releasing and controlled releasing functional microcapsule |
| CN1410055A (en) * | 2002-06-06 | 2003-04-16 | 上海家化联合股份有限公司 | Vitamin A liposome and its preparation method |
| CN101502326A (en) * | 2009-02-23 | 2009-08-12 | 江南大学 | Method for preparing vitamin E nano liposome |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6361800B1 (en) * | 2000-04-13 | 2002-03-26 | Cooper Concepts, Inc. | Multi-vitamin and mineral supplement |
| CN1843327A (en) * | 2006-04-17 | 2006-10-11 | 重庆医药工业研究院有限责任公司 | Stable freeze-dried formulation containing multiple kinds of vitamin and its preparation method |
| CN101564384B (en) * | 2008-07-14 | 2012-03-07 | 烟台绿叶动物保健品有限公司 | Multivitamin aqueous solution preparation |
| CN101491499B (en) * | 2009-02-19 | 2010-10-13 | 陶灵刚 | Composite liposome for injection containing 12 vitamins and preparation method thereof |
| CN101822320B (en) * | 2010-05-22 | 2013-07-03 | 鼎正动物药业（天津）有限公司 | Vitamin water aqua and preparation method thereof |
-
2010
- 2010-05-22 CN CN2010101796369A patent/CN101822320B/en active Active
-
2011
- 2011-05-20 KR KR1020127021887A patent/KR20130083822A/en not_active Application Discontinuation
- 2011-05-20 AU AU2011257786A patent/AU2011257786A1/en active Pending
- 2011-05-20 AU AU2011101750A patent/AU2011101750A4/en not_active Ceased
- 2011-05-20 WO PCT/CN2011/074437 patent/WO2011147281A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1096194A (en) * | 1993-06-07 | 1994-12-14 | 北京和德实业公司 | The cosmetics of the lecithin liposome of embedding vitamin and method for making thereof |
| CN1225815A (en) * | 1998-06-10 | 1999-08-18 | 南方医院 | Dried lipid article and preparation method therefor |
| CN1364459A (en) * | 2001-07-02 | 2002-08-21 | 刘银良 | Method for preparing slow-releasing and controlled releasing functional microcapsule |
| CN1410055A (en) * | 2002-06-06 | 2003-04-16 | 上海家化联合股份有限公司 | Vitamin A liposome and its preparation method |
| CN101502326A (en) * | 2009-02-23 | 2009-08-12 | 江南大学 | Method for preparing vitamin E nano liposome |
Non-Patent Citations (3)
| Title |
|---|
| 刘亚文等.维生素C脂质体的制备研究.《大豆科学》.2007,第26卷(第2期), * |
| 张伟光等.大豆磷脂维生素E脂质体的制备.《食品工业科技》.2007,第28卷(第5期), * |
| 李国锋等.维生素E脂质体的制备.《中国现代应用药学》.1997,第14卷(第4期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2011101750A4 (en) | 2015-02-05 |
| WO2011147281A1 (en) | 2011-12-01 |
| AU2011257786A1 (en) | 2012-08-30 |
| CN101822320A (en) | 2010-09-08 |
| KR20130083822A (en) | 2013-07-23 |
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