CN101803634A - Kelp dietary fiber yoghourt and production method thereof - Google Patents

Kelp dietary fiber yoghourt and production method thereof Download PDF

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Publication number
CN101803634A
CN101803634A CN 200910019182 CN200910019182A CN101803634A CN 101803634 A CN101803634 A CN 101803634A CN 200910019182 CN200910019182 CN 200910019182 CN 200910019182 A CN200910019182 A CN 200910019182A CN 101803634 A CN101803634 A CN 101803634A
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dietary fiber
kelp dietary
kelp
tangle
sucrose
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CN101803634B (en
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孟秀梅
刘昌衡
唐聚德
张绵松
袁文鹏
夏雪奎
王小军
孙永军
胡炜
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Biology Institute of Shandong Academy of Sciences
Shandong Homey Aquatic Development Co Ltd
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Biology Institute of Shandong Academy of Sciences
Shandong Homey Aquatic Development Co Ltd
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Abstract

The invention relates to a kelp dietary fiber yoghourt and a production method thereof. The production method comprises the following steps: adding a water solution of kelp dietary fibers, stabilizer and sucrose to weighed fresh milk, wherein the stabilizer comprises sodium carboxymethylcellulose and xanthan gum, and the mass ratio of the sodium carboxymethylcellulose to the xanthan gum is 2:1; and homogenizing, sterilizing, cooling, then adding strains to ferment, and cooling to obtain the kelp dietary fiber yoghourt, wherein the strains comprise Streptococcus thermophilus and Lactobacillus bulgaricus, and the mass ratio of the Streptococcus thermophilus to the Lactobacillus bulgaricus is 1:1; and the kelp dietary fiber yoghourt comprises the following components in percentage by weight: 80-90% of the fresh milk, 1-3% of the kelp dietary fibers, 4-10% of the sucrose, 0.3-0.8% of the stabilizer, 1-5% of strains and balance of water. The kelp dietary fiber yoghourt is sweet and sour, is rich in nutritive materials, and can smooth intestinal tract, prevent and cure constipation, reduce cholesterol and regulate blood fat and blood sugar in case of being drunk for a long term, and is especially applicable to the diet of middle-aged and elderly people, diabetes patients and overweight people. The production method has the advantages of advanced process, reasonable steps and easy operation, and is applicable to industrialized production.

Description

A kind of kelp dietary fiber yoghourt and production method thereof
Technical field
The present invention relates to a kind of preparation that uses the cultured milk of additive, especially a kind of kelp dietary fiber yoghourt and production method thereof with kelp dietary fiber and the preparation of milk combining with fermentation.
Background technology
We know, yoghurt is to be raw material with fresh milk, through adding beneficial bacterium (leavening) again after the Ma Shi sterilization in milk, makes a kind of milk product after fermentation, and its taste is sour-sweet fine and smooth, is loved by the people.Sugar in the sweat milk, protein have and are decomposed into little molecule (as galactolipin and lactic acid, little peptide chain and amino acid etc.) about 20%; Aliphatic acid in the yoghurt after the fermentation has increased by 2 times than raw material milk; Also can produce multivitamin in the sweat, as VB1, VB2, VB6, VB12 etc.Compare with fresh milk, sour milk not only has whole nutrients of fresh milk, and sour milk can make protein form trickle newborn piece, lactic acid and calcium are in conjunction with the calcium lactate that generates, easier being digested and assimilated, its lactic acid bacteria that contains has and promotes gastric secretion, improves appetite, strengthens the effect of digestion, and can secrete the material useful to health during breeding in human body intestinal canal, keeps intestinal tract normal flora clump balance.
Dietary fiber is the dietary nutrient that a kind of difficult quilt digests, and mainly comes from the cell membrane of plant.It is playing the part of important role on maintenance digestive system health, can clean wall of digestive and strengthen digestive function, and alimentary canal and prevention colon cancer are protected in removing of carcinogen in dilution and the acceleration food and noxious material; And can slow down digestion rate and rapid drainage cholesterol, allow blood sugar and cholesterol in the blood be controlled at desirable level.Therefore, the enough fiber of picked-up also can angiocardiopathy preventing, cancer, diabetes and other disease.
In recent years, dietary fiber is applied to existing report in the dairy produce.For example, in the disclosed CN101438738A Chinese invention patent ublic specification of application on May 27th, 2009 disclosed " a kind of hypoglycemic sour milk and production method thereof " it is made up of degreasing fresh milk, water-soluble dietary fiber, xylitol, lactalbumin, stabilizing agent, Aspartame, acesulfame potassium, trivalent chromium, streptococcus thermophilus, lactobacillus delbruockii subspecies bulgaricus.Trivalent chromium in this sour milk plays the auxiliary health-care effect that reduces blood sugar.And for example, June 10 in 2009 Granted publication CN100496263C Chinese invention patent specification in disclosed " hypoglycemic sour milk ", it comprises raw milk, ferment agent for sour milk and additive, additive is a Cobastab 1, Cobastab 2, niacinamide, calcium pantothenate, trivalent chromium, FOS, soluble dietary fiber, xylitol.
The effect that it has the protection islet function, improves glycometabolism, reduces blood pressure and blood lipoid and delay and prevent the generation and the development of complication at the characteristics of diabetic supersession, provides the diabetes patient dairy produce.
The nutritive value of sea-tangle is very high, contains abundant algin, sweet mellow wine, iodine, cellulose and mineral matter etc.The dietary fiber that extracts from sea-tangle (Dietaryfiber) is water-insoluble dietary fiber, its physiological function has been compared a great difference with the dietary fiber that land plant is produced, it has the stronger oil of holding, moisture holding capacity, solubilising and induce action of microorganisms, the energy huge legendary turtle is closed the cholesterol in the alimentary canal, harmful substances such as heavy metal, reduce carcinogenic generation and promote enterocinesia, prevention colon cancer and constipation, simultaneously, in prevention with improve the heart disease that coronary sclerosis causes, regulate the blood sugar level and the aspects such as prevention of obesity disease and gall stone of diabetes, have unique health-care effect.
At present, yet there are no and be the report that kelp dietary fiber and milk composite fermentation prepare kelp dietary fiber yoghourt.
Summary of the invention
In order to solve deficiency of the prior art, the invention provides a kind of kelp dietary fiber yoghourt and production method thereof.This kelp dietary fiber yoghourt is sweet mouthfeel, nutritious not only, and has comprised the health care of dietary fiber, but angiocardiopathy preventing, cancer, obesity and adjusting blood sugar level.Its production method technology is advanced rationally, strong operability, is suitable for suitability for industrialized production
The technical solution adopted for the present invention to solve the technical problems is: a kind of kelp dietary fiber yoghourt, it is characterized in that: it includes fresh milk, kelp dietary fiber, sucrose, stabilizing agent, bacterial classification and water, the percentage by weight of described said components is respectively: fresh milk 80~90%, kelp dietary fiber 1~3%, sucrose 4~10%, stabilizing agent 0.3~0.8%, bacterial classification 1~5%, surplus is a water, and the percentage by weight sum of described component is 100%.
Described stabilizing agent is the mixture of sodium carboxymethylcellulose and xanthans, and its ratio is 2: 1.
Described bacterial classification is the Mixed Microbes that streptococcus thermophilus and lactobacillus bulgaricus constitute, and its mixed proportion is 1: 1.
A kind of production method of kelp dietary fiber yoghourt is characterized in that: through the following step
(1), the preparation of kelp dietary fiber:
Choose sea-tangle after clean, drying, pulverizing, in pH value 4~5, temperature (50 ± 2) ℃, adopt cellulase and protease hydrolyzed 0.5~2h, to destroy the cell membrane of sea-tangle, discharge algin, wherein the consumption of cellulase and protease is: every gram sea-tangle dry powder adopts alive the living for cellulase 10~15u of 20000u/g, enzyme of enzyme to be protease 18~25u of 100000u/g; Adopting 18~20 times of dried sea-tangle weight, concentration then is 3~8% sodium carbonate liquor, and under temperature (55 ± 5) ℃ condition, the alkaline hydrolysis time is 1~3h, and the protein with in the alkaline hydrolysis digestion sea-tangle enzymolysis liquid is further purified kelp dietary fiber; Adopting 10~15 times of dried sea-tangle weight, concentration again is 1~3% liquor natrii hypochloritis, and 10~30min is handled in bleaching to kelp dietary fiber; Adopt 2~3 times of dried sea-tangle weight, concentration to dewater again for the kelp dietary fiber of the edible ethanol of (95 ± 5) % after bleaching is handled; Kelp dietary fiber after will dewatering at last places vacuum drying chamber (55 ± 5) ℃ vacuum drying, ultramicro grinding 200 orders that sieve, kelp dietary fiber, standby;
(2), the preparation of stabilizing agent and aqueous sucrose solution
Weighing stabilizing agent sodium carboxymethylcellulose and xanthans, both ratios are 2: 1; The stabilizing agent of weighing sucrose and above-mentioned weighing mixes in proportion, and adds water and at 60-65 ℃ it is fully dissolved, its aqueous solution, standby;
(3), the preparation of mixed liquor and processing
The aqueous solution of the kelp dietary fiber of weighing fresh milk, above-mentioned preparation, the stabilizing agent of above-mentioned preparation and sucrose is poured in the container, after stirring, be placed on and carry out homogeneous under 50-75 ℃, 15-25MPa and handle, mixed liquor;
(4), fermentation
With the mixed liquor behind the above-mentioned homogeneous under 85~100 ℃ of temperature conditions, sterilization 10~20min, pour inoculation container then into, be cooled to 40~45 ℃, insert the Mixed Microbes of streptococcus thermophilus and lactobacillus bulgaricus, both ratios are 1: 1, stir, be placed on 43 ℃ of bottom fermentation 3~5h after, take out and to place air to naturally cool to room temperature, be placed on 0~4 ℃ of refrigeration of freezer, 12~24h then, promptly get dietary fiber yoghourt.
The used water of described steady dissolution agent and sucrose is demineralized water.
Kelp dietary fiber yoghourt of the present invention is to add kelp dietary fiber, sucrose and stabilizing agent in fresh milk, homogeneous, and sterilization, the cooling back adds strain fermentation, cooling forms.In dairy produce, added kelp dietary fiber, satisfied people to nutritional labeling demands such as protein, vitamin A, fat in, increased the health care of dietary fiber again, enlarged the scope of application of dairy produce.This kelp dietary fiber yoghourt sweet mouthfeel, nutritious, long-term drinking can make enteron aisle unobstructed, anti-treating constipation, and can reduce cholesterol, and regulate blood fat, blood sugar, be particularly suitable for the elderly, diabetic and overweight people and eat.The production method technology advanced person of this kelp dietary fiber yoghourt, step is reasonable, and strong operability is suitable for suitability for industrialized production.
The specific embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
The component of kelp dietary fiber yoghourt and percentage by weight are respectively in the present embodiment: fresh milk 80%, kelp dietary fiber 3%, sucrose 9%, stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) 0.5%, bacterial classification (streptococcus thermophilus: lactobacillus bulgaricus=1: 1) 2%, surplus is a water.
The production method of kelp dietary fiber yoghourt is in the present embodiment:
Get that sea-tangle is cleaned, after the drying and crushing, in pH value 4.0,48 ℃ of temperature, adopt cellulase and protease to carry out enzymolysis processing 2h, it with the enzyme amount is: every gram sea-tangle dry powder needs alive the living for the cellulase 10u of 20000u/g, enzyme of enzyme to be the protease 18u of 100000u/g, cell membrane through the sea-tangle behind the enzymolysis doubly destroys, and algin fully obtains discharging; Adopt then 18 times of dried sea-tangle weight, concentration be 3% sodium carbonate liquor 60 ℃ of temperature, the alkaline hydrolysis time is 1h, by catapepsis, helps being further purified of dietary fiber through the protein in the alkaline hydrolysis sea-tangle enzymolysis liquid; Adopting 10 times of dried sea-tangle weight, concentration again is 1% liquor natrii hypochloritis, to kelp dietary fiber bleaching 30min; Adopting 2 times of dried sea-tangle weight, concentration again is that kelp dietary fiber after 100% edible ethanol is handled bleaching dewaters; Be placed on 50 ℃ of vacuum drying in the vacuum drying chamber at last, ultramicro grinding 200 orders that sieve, kelp dietary fiber, standby.
With stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) mix according to the above ratio with sucrose, and add demineralized water and it is fully dissolved at 60 ℃, its aqueous solution, standby.
Weighing is poured in the container by above-mentioned percentage by weight through the aqueous solution of fresh milk, the above-mentioned kelp dietary fiber that makes, stabilizing agent and the sucrose of filtration, stir, be placed on then under 65 ℃, 20MPa condition, carry out homogeneous and handle, get mixed liquor, standby.
Under 95 ℃ of temperature, pour the mixed liquor behind the homogeneous into inoculation container behind the sterilization 15min, insert bacterial classification (streptococcus thermophilus: lactobacillus bulgaricus=1: 1), stir by above-mentioned percentage by weight when being cooled to 43 ℃.
Be placed on 43 ℃ of bottom fermentation 5h after the inoculation, taking-up places air to naturally cool to room temperature, is placed on freezer 0-4 ℃ of refrigeration 20h then, promptly gets dietary fiber yoghourt.
Embodiment 2
The component of kelp dietary fiber yoghourt and percentage by weight are respectively in the present embodiment: fresh milk 85%, kelp dietary fiber 1%, sucrose 6%, stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) 0.8%, bacterial classification (streptococcus thermophilus: lactobacillus bulgaricus=1: 1) 4%, surplus is a water.
The production method of kelp dietary fiber yoghourt is in the present embodiment:
Get that sea-tangle is cleaned, after the drying and crushing, in pH value 4.5,50 ℃ of temperature, adopt cellulase and protease to carry out enzymolysis processing 1h, it with the enzyme amount is: every gram sea-tangle dry powder needs alive the living for the cellulase 12u of 20000u/g, enzyme of enzyme to be the protease 20u of 100000u/g, cell membrane through the sea-tangle behind the enzymolysis doubly destroys, and algin fully obtains discharging; Adopt then 19 times of dried sea-tangle weight, concentration be 5% sodium carbonate liquor 55 ℃ of temperature, the alkaline hydrolysis time is 2h, by catapepsis, helps being further purified of dietary fiber through the protein in the alkaline hydrolysis sea-tangle enzymolysis liquid; Adopting 12 times of dried sea-tangle weight, concentration again is 2% liquor natrii hypochloritis, and to kelp dietary fiber bleaching 20min, adopting 2.5 times of dried sea-tangle weight, concentration is that kelp dietary fiber after 95% edible ethanol is handled bleaching dewaters; Be placed on 55 ℃ of vacuum drying in the vacuum drying chamber at last, ultramicro grinding 200 orders that sieve, kelp dietary fiber, standby.
With stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) mix according to the above ratio with sucrose, and add demineralized water and it is fully dissolved at 63 ℃, its aqueous solution, standby.
Weighing is poured in the container according to the above ratio through the aqueous solution of the fresh milk, the above-mentioned kelp dietary fiber that makes, stabilizing agent and the sucrose that filter, stirs, and is placed on then under 75 ℃, 15MPa condition, carries out homogeneous and handles, must mixed liquor, and standby.
Under 85 ℃ of conditions, pour the mixed liquor behind the homogeneous into inoculation container behind the sterilization 20min, insert bacterial classification (streptococcus thermophilus: lactobacillus bulgaricus=1: 1), stir by above-mentioned weight ratio when being cooled to 43 ℃.
Be placed on 43 ℃ of bottom fermentation 3h after the inoculation, taking-up places air to naturally cool to room temperature, is placed on freezer 0-4 ℃ of refrigeration 12h then, promptly gets dietary fiber yoghourt.
Embodiment 3
The component of kelp dietary fiber yoghourt and percentage by weight are respectively in the present embodiment: fresh milk 90%, kelp dietary fiber 2%, sucrose 4%, stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) 0.3%, bacterial classification (streptococcus thermophilus: lactobacillus bulgaricus=1: 1) 1%, surplus is a water.
The processing method of kelp dietary fiber yoghourt is in the present embodiment:
Get that sea-tangle is cleaned, after the drying and crushing, in pH value 5,52 ℃ of temperature, adopt cellulase and protease to carry out enzymolysis processing 0.5h, it with the enzyme amount is: every gram sea-tangle dry powder needs alive the living for the cellulase 15u of 20000u/g, enzyme of enzyme to be the protease 25u of 100000u/g, cell membrane through the sea-tangle behind the enzymolysis doubly destroys, and algin fully obtains discharging; Adopt then 20 times of dried sea-tangle weight, concentration be 8% sodium carbonate liquor 50 ℃ of temperature, the alkaline hydrolysis time is 3h, by catapepsis, helps being further purified of dietary fiber through the protein in the alkaline hydrolysis sea-tangle enzymolysis liquid; Adopting 15 times of dried sea-tangle weight, concentration again is 3% liquor natrii hypochloritis, kelp dietary fiber is bleached handled 10min; Adopting 3 times of dried sea-tangle weight, concentration is that 90% edible ethanol is handled the back kelp dietary fiber to bleaching and carried out processed; Be placed on 60 ℃ of vacuum drying in the vacuum drying chamber at last, ultramicro grinding 200 orders that sieve, kelp dietary fiber, standby.
With stabilizing agent (sodium carboxymethylcellulose: xanthans (w/w)=2: 1) mix according to the above ratio with sucrose, and the water that adds after softening fully dissolves it at 65 ℃, its aqueous solution, standby.
Weighing is poured in the container by above-mentioned weight ratio through the mixed liquor of the fresh milk, the above-mentioned kelp dietary fiber that makes, stabilizing agent and the sucrose that filter, stirs, and is placed on then to carry out homogeneous under 50 ℃, 25MPa condition and handle, must mixed liquor, and standby.
Under 100 ℃ of temperature conditions, pour the mixed liquor behind the homogeneous into inoculation container behind the sterilization 10min, insert bacterial classification by above-mentioned weight ratio when being cooled to 45 ℃, stir.
Be placed on 43 ℃ of bottom fermentation 4h after the inoculation, taking-up places air to naturally cool to room temperature, is placed on freezer 0-4 ℃ of refrigeration 18h then, promptly gets dietary fiber yoghourt.
Kelp dietary fiber yoghourt provided by the invention, its kelp dietary fiber can improve the drink mouthfeel in dairy produce, improve stability, and does not take place the disadvantageous physical and chemical reaction of human body with any composition wherein; Drink the back and strengthen satiety, minimizing is to the intake of heat material, long-term drinking can significantly help the control fat-reducing, and make enteron aisle happy, and can reduce cholesterol, adjusting blood fat, blood sugar, anti-treating constipation, the generation of angiocardiopathy preventing and colon cancer is particularly suitable for the elderly, diabetic and overweight people and drinks.

Claims (5)

1. kelp dietary fiber yoghourt, it is characterized in that: it includes fresh milk, kelp dietary fiber, sucrose, stabilizing agent, bacterial classification and water, the percentage by weight of described said components is respectively: fresh milk 80~90%, kelp dietary fiber 1~3%, sucrose 4~10%, stabilizing agent 0.3~0.8%, bacterial classification 1~5%, surplus is a water, and the percentage by weight sum of described component is 100%.
2. a kind of kelp dietary fiber yoghourt according to claim 1 is characterized in that: described stabilizing agent is the mixture of sodium carboxymethylcellulose and xanthans, and its ratio is 2: 1.
3. a kind of kelp dietary fiber yoghourt according to claim 1 and 2 is characterized in that: described bacterial classification is the Mixed Microbes that streptococcus thermophilus and lactobacillus bulgaricus constitute, and its ratio is 1: 1.
4. the production method of a kind of kelp dietary fiber yoghourt according to claim 1 is characterized in that: through the following step
(1), the preparation of kelp dietary fiber:
Choose sea-tangle after clean, drying, pulverizing, in pH value 4~5, temperature (50 ± 2) ℃, adopt cellulase and protease hydrolyzed 0.5~2h, to destroy the cell membrane of sea-tangle, discharge algin, wherein the consumption of cellulase and protease is: every gram sea-tangle dry powder adopts alive the living for cellulase 10~15u of 20000u/g, enzyme of enzyme to be protease 18~25u of 100000u/g; Adopting 18~20 times of dried sea-tangle weight, concentration then is 3~8% sodium carbonate liquor, and under temperature (55 ± 5) ℃ condition, the alkaline hydrolysis time is 1~3h, and the protein with in the alkaline hydrolysis digestion sea-tangle enzymolysis liquid is further purified kelp dietary fiber; Adopting 10~15 times of dried sea-tangle weight, concentration again is 1~3% liquor natrii hypochloritis, and 10~30min is handled in bleaching to kelp dietary fiber; Adopt 2~3 times of dried sea-tangle weight, concentration to dewater again for the kelp dietary fiber of the edible ethanol of (95 ± 5) % after bleaching is handled; Kelp dietary fiber after will dewatering at last places vacuum drying chamber (55 ± 5) ℃ vacuum drying, ultramicro grinding 200 orders that sieve, kelp dietary fiber, standby;
(2), the preparation of stabilizing agent and aqueous sucrose solution
Weighing stabilizing agent sodium carboxymethylcellulose and xanthans, both ratios are 2: 1; The stabilizing agent of weighing sucrose and above-mentioned weighing mixes in proportion, and adds water and at 60-65 ℃ it is fully dissolved, its aqueous solution, standby;
(3), the preparation of mixed liquor and processing
The aqueous solution of the kelp dietary fiber of weighing fresh milk, above-mentioned preparation, the stabilizing agent of above-mentioned preparation and sucrose is poured in the container, after stirring, be placed on and carry out homogeneous under 50-75 ℃, 15-25MPa and handle, mixed liquor;
(4), fermentation
With the mixed liquor behind the above-mentioned homogeneous under 85~100 ℃ of temperature conditions, sterilization 10~20min, pour inoculation container then into, be cooled to 40~45 ℃, insert the Mixed Microbes of streptococcus thermophilus and lactobacillus bulgaricus, both ratios are 1: 1, stir, be placed on 43 ℃ of bottom fermentation 3~5h after, take out and to place air to naturally cool to room temperature, be placed on 0~4 ℃ of refrigeration of freezer, 12~24h then, promptly get dietary fiber yoghourt.
5. the production method of a kind of kelp dietary fiber yoghourt according to claim 4, it is characterized in that: the used water of described steady dissolution agent and sucrose is demineralized water.
CN 200910019182 2009-09-25 2009-09-25 Kelp dietary fiber yoghourt and production method thereof Expired - Fee Related CN101803634B (en)

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CN106720337A (en) * 2016-11-11 2017-05-31 青岛旭西辰网络技术有限公司 A kind of health-care algae Yoghourt
CN106578077A (en) * 2016-11-21 2017-04-26 北京世纪博康医药科技有限公司 Milk tea containing kelp dietary fibers and preparation method thereof
CN107937317A (en) * 2017-12-26 2018-04-20 山东得益乳业股份有限公司 Lactobacillus delbruockii subspecies bulgaricus, its application and Yoghourt prepared therefrom
CN107937317B (en) * 2017-12-26 2021-02-05 山东得益乳业股份有限公司 Lactobacillus delbrueckii subsp bulgaricus, application thereof and yoghourt prepared from lactobacillus delbrueckii subsp bulgaricus
CN109136303A (en) * 2018-08-31 2019-01-04 中国海洋大学 A kind of preparation method of the seaweed diet fiber rich in algin oligosaccharide
CN110787191A (en) * 2019-11-25 2020-02-14 自然资源部第一海洋研究所 Application of soluble dietary fiber in kelp in preparation of lipid-lowering and weight-losing medicine and functional food
CN112998075A (en) * 2021-03-23 2021-06-22 大连工业大学 Production method of kelp yoghourt with eye protection function
CN113647461A (en) * 2021-08-20 2021-11-16 成都市农林科学院 Double-protein yoghourt containing natto hormone and preparation method thereof

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