CN101785852A - Dermis trace intervention anti-wrinkle cosmetic preparation and method - Google Patents
Dermis trace intervention anti-wrinkle cosmetic preparation and method Download PDFInfo
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- CN101785852A CN101785852A CN201010034390A CN201010034390A CN101785852A CN 101785852 A CN101785852 A CN 101785852A CN 201010034390 A CN201010034390 A CN 201010034390A CN 201010034390 A CN201010034390 A CN 201010034390A CN 101785852 A CN101785852 A CN 101785852A
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Abstract
The invention discloses a cosmetic method for repairing skin wrinkle and pitting defect, which includes that dermis trace intervention surgery is adopted to deliver composite preparation which contains biological active components and consists of recombinant human Platelet-Derived Growth Factor (rhPDGF) with dermis repairing function, Transforming Growth Factor (TGF) and Hyaluronic Acid (HA) into dermis for stimulating the growth and mitosis of fibroblast, the synthesis and secretion of collagen and HA, the generation and repairing of original fiber and flexibility fiber so that the skin defects of skin pitting, deep wrinkle and dermis fiber tissue fracture are repaired for a long term, and the skin beautifying and anti-aging effect are realized.
Description
Technical field
The invention discloses the new purposes of beauty treatment of a kind of recombined human transforming growth factor and derivative growth factor of recombined human blood platelet, the technical field that patent relates to is the application in beautifying skin and defying age field of interventional medicine and medicine biological technique.
Background technology
Skin is the organ of human body maximum, 5%～15% of gross weight percentage of liveweight, and the gross area is 1.5～2 square metres.Skin has the stable function of the human internal environment of maintenance; prevent the barrier action of losing and stop the intrusion of extraneous harmful substance of moisture, electrolyte and other nutrient substance in the body; on physiology, play important defencive function; skin also is health and old and feeble vital sign and sign simultaneously, has important beauty treatment function.The damage of skin happens occasionally, and skin aging is inevitable.
It is sagging that skin aging begins to show as cutis laxa, because circulation ability weakens, begins around eye, forehead, the cervical region to occur expanding, and the corium connective tissue loses normal function, and skin loses soft moist outward appearance, and dissolubility collagen disappears and makes pachylosis; The skin elasticity collagen protein that runs off gradually, skin mound structure can not swell and keep shape; Reasons such as sebum secretion reduction cause xerosis cutis, and skin liquid hold-up ability goes down; Elastic fibers of skin is rotten, and wrinkle of skin begins to occur and deepen year by year.Anatomical research shows that corium fibroblast quantity reduces gradually after 20 years old, and the collagen protein total content is annual to reduce 1%, the collagen fiber chap, and density increases, and becomes insolubility, is difficult for being decomposed by collagenase, and collagen stability increases.Relevant collagen gene expression research discloses, and type i collagen gene expression reduces, and type i collagen is synthetic to be reduced, and the gene expression of III Collagen Type VI increases.Because these changes of collagen fiber weaken the conduction of collagen stress, in case be subjected to undue the stretching that lacerated wound just takes place easily.The reticular layer of corium elastic fiber is arranged and is changed, fiber obscurity boundary under the Electronic Speculum, and accidental fibrous fracture, these variations were seen after 50 years old more.The contained microfilament of elastic fiber reduces, and the inclusions of elastic fiber increases, elastic fiber morphological variation complexity, cause skin stretch after elastic-restoring force weaken.The papillary layer of corium elastic fiber also changes, and normally rises to the elastic webs of being close under the basement membrane dermal papilla extreme higher position and has disappeared.Electron microscopic examination shows that improper elastic fiber is made of a large amount of microfibre precipitations, and the degeneration of this elastic fiber causes cutis laxa, forms tiny wrinkle.Because the structural change of collagen and elastic fiber causes the change of skin biomechanics.The skin expansion service measured by the utilization machinery and elastic-restoring force is found, the skin expansion service just obviously descended after 70 years old, and elastic-restoring force is from beginning decline very early.So skin elasticity can be used as a parameter weighing the skin aging degree.Skin tension obviously descends after 35 years old.
Aging, over-drying environment, pull factor such as skin undeservedly, all can destroy the structure of collagen protein, cause the appearance of fine line even wrinkle.Collagen protein is as follows in the transformation of skin skin corium structure, by " single Shu Lianjie (the intrastrand links) " of cambium, become " bundle and Shu Lianjie (interstrand links) ", the mode that becomes " intermolecular binding (intermolecular links) " at last exists.Therefore the overall structure of collagen protein just becomes comparatively closely that but support force weakens relatively, causes the tension force of skin corium to reduce thereupon, will make the skin corium elastin lose elasticity gradually, makes skin present slack and undisciplined outward appearance.And the physico-chemical property of collagen protein equally also can change to some extent, and the collagen protein of cambium is water solublity, and the collagen protein of aging tissue then is water-insoluble.Relatively collagen protein content in vivo then can be found, the survival age, the long more interior proteic total content of collagen of its body of animal can be few more, and the ratio of water-insoluble collagen protein also can increase thereupon.
Solve at present that wrinkle of skin and old and feeble mode mainly comprise 1, the nutrient substance of oral defying age and additional loss composition, comprise vitamin C, vitamin E, collagen protein, hyaluronic acid (hyaluronic acid) etc., perhaps 2, adopt injection creotoxin, Elipten AG, hyaluronic acid, collagen protein etc. to replenish or suppress the medicine or the nutritional preparation of loss composition.Oral way is very complicated and uncontrollable by the process that digestive system and metabolism arrive skin corium, can only have generalized Nutrition from threpsology's angle, lack the support of clinical data, particularly can't accomplish the specific aim reparation specific skin injury and deep wrinkle.The injection system targeting is strong, and effect is remarkable, but cost is high, or has in various degree side effect as creotoxin, Elipten AG etc., or as hyaluronic acid, collagen protein etc. because of being absorbed by perienchyma or shorter with the intercellular substance loss maintenance phase.
Cell growth factor is that a class is by combining the polypeptides matter of manifold effects such as the growth of adjusting cell and other cell functions with special, high affine cell-membrane receptor.Be present in platelet and various adult and embryonal tissue and the most of cultured cell, the variety classes cell is had certain specificity.On secretion characteristic, somatomedin mainly belongs to autocrine (autocrine) and paracrine (paracrine).Many somatomedin have been purified and have been determined its structure composition.All kinds of somatomedin all have its corresponding receptor, are the transmembrane proteins that is prevalent on the cell membrane, and many receptors have kinase activity, particularly tyrosine kinase activity (as pdgf receptor, EGF receptor etc.).Because somatomedin is by the normal cell secretion, has not both had medicine class toxicity, does not also have immunoreation, therefore in its physiological action mechanism of research simultaneously, what have tries out in clinical treatment.Somatomedin has multiple, as platelet class somatomedin (platelet source somatomedin, PDGF; Osteosarcoma source somatomedin ODGF), epidermal growth factor subclass (epidermal growth factor, EGF, transforming growth factor, TGF α and TGF β), fibroblast growth factor (α FGF, β FGF), insulin like growth factor (IGF-I, IGF-II), nerve growth factor (NGF), interleukin class somatomedin (IL-1, IL-1, IL-3 etc.), erythropoietin (EPO), colony stimulating factor (CSF) etc.
PDGF is the endogenous protein and peptide with duplex structure of human body, form by 109 aminoacid, 8 pairs of disulfide bond are arranged in the molecular structure, molecular weight is 24～30KDa, be stored in the platelet with the particulate form of α, the PDGF concentration that exists among the human normal plasma is about 17.5+/-3.1 (SD) ng/mL, and is capable with blood circulation.During tissue damaged, the inflammatory cell of macrophage, platelet, infiltration, impaired endotheliocyte and activated stellate cells all can be secreted PDGF, play a role in the mode of autocrine, paracrine.PDGF is the main mitosis source that secretion of platelet discharges, and is a very important somatomedin that acts on mesenchymal cell, directly promotes Normocellular propagation and differentiation, and reversing the differentiation aged cells is young stem cell, can recover cell viability.The PDGF of denier is the variation in the trigger cell phase of the cycles strongly, the DNA of irritation cell is synthetic, cause the synthetic and mitosis of RNA, delay the normal cell senescence process, prolong the life cycle of cell, quicken the apoptosis process of mutant, promote the renewal and the regeneration of cell, has good promotion cel l proliferation, regenerative cell is increased gradually, PDGF can bring out the formation of other cell growth factor (hEGF, hbFGF, hNGF, aFGF, TGF-β) simultaneously, promotes the various types of cells growth comprehensively.
Dermatology studies show that rhPDGF can effectively strengthen cell viability, promotes the metabolism of skin histology, makes the skin histology progressive additive, and the normal skin structure is recovered gradually.Reparation for propagation, regeneration and the damaged skin tissue of epidermis cell has extremely strong effect, promote the division of various skin cells such as fibroblast, epidermis cell, neurocyte and the regeneration of new skin histology, promote the formation of collagen fiber, reticular fiber, elastic fiber.Through using after a while, can effectively repair the damaged skin tissue, obviously recover, strengthen skin elasticity, unfold wrinkle, slow down aging is returned to skin and compacts and whippy young state.RhPDGF can induce formation, growth and the differentiation of capillary of skin, microcirculation improvement effectively provides the skin desired nutritional, strengthens Skin Cell to the absorption of nutrient substance with to the drainage of metabolic waste, promote skin vitality, delaying decrepitude of skin strengthens the resistance to environment invasion and attack, ultraviolet, pollutant, stimulus object, sensitizer, inflammatory cell, and prevention acne, skin sore take place, the desalination facial colour spot, improve facial dark and gloomy color and luster, the nourishing skin is white touched with red, and is young vigorous and graceful.The outer macromolecular synthetic and secretion (as hyaluronic acid, glycoprotein etc.) of rhPDGF energy irritation cell, promote the growth of human body self collagen protein, hyaluronic acid and fibrinous synthetic and collagen fiber, reticular fiber, elastic fiber, regulate the secretion of collagen protein and mucopolysaccharide, keep moisture and electrolyte metabolism in the skin histology, improve the exsiccosis of atrophy skin, make Skin Cell fuller, intercellular substance is more full, eliminate or the minimizing wrinkle, conditioning skin children is tender smooth, and is moist plentiful.
RhPDGF is to the reparation and the beautification function of Skin Cell:
RhPDGF adopts technique for gene engineering expression and highly purified through modern chromatography, to human skin cell without any toxic and side effects, be unique gene engineering product in numerous cell growth factor, be widely used in the treatment that diabetic ulcer healing, tissue regeneration, movable joint shaping such as rescue at the aspect at present abroad by drugs approved by FDA listing.Shang Weiyou sees its research and application aspect beautifying skin reparation and defying age.RhPDGF is skin corium tissue defect and old and feeble excellent repairing composition, regulates and reproduces in the face of the microstructure of skin histologies such as fibroblast, angle unit cell at cellular layer, has broad application prospects in beautifying and antisenility and beauty and shaping field.
TGF-β is the multiple tissue of a kind of parent, multiple effect and polyfunctional cytokine.It is produced by mesenchymal cell, also can be produced by the lymphocyte that soaks into, mononuclear phagocyte and platelet.The binary that TGF-β borrows disulfide bond to connect by two structure 12.5kDa subunits identical or close, molecular weight, its cDNA sequence studies show that monomeric TGF-β 112 amino acid residues are from the c-terminus cracking by the precursor molecule that contains 400 amino acid residues (per-pro-TGF-β).Pre-pro-TGF-β N end contains a signal peptide, cleavedly before secretion fall, become the polypeptide chain precursor (pro-TGF-β) of inactive state, by changing ionic strength, acidify or protease hydrolysis excision N end parts amino acid residue, remaining c-terminus partly is formed with active TGF-β.Originally the biological function of TGF-β is studied mainly at aspects such as inflammation, tissue repair and fetal development, growth, differentiation and the immunologic function of discovered in recent years TGF-β pair cell all have important regulatory role.TGF-β 1, β 2 and β 3 functional similarities, in general, the super stimulation of cell that TGF-β originates to mesenchyme, and the cell in epithelium or neuroderm source is played inhibitory action: promote the growth of fibroblast, osteoblast and Schwann cell.TGF-β 1, TGF-β 2 promote the generation of human fibroblasts IL-6, and its mechanism may be by the adjusting to the IL-6 genetic transcription; Suppress the formation of epithelial cell, osteoclast, endothelial cell growth and fat, cardiac muscle, skeletal muscle.But some biological function of TGF-β antagonism EGF; Promote extracellular matrix (ECM) as the expression of collagen protein, fibronectin and the degraded of inhibition ECM, form generation, propagation and the atomization of pair cell play an important role, and help fetal development and cytothesis.Experiment shows in the animal body, and local injection TGF-β can promote wound healing and typical granulation tissue to form.Too much TGF-β can make tissue fibering, can cause the fibroblast migration, suppresses collagenase and other MMP activities, increases collagen and produces, and reduces collagen degradation, promotes apposition.
The present invention can stimulate rhPDGF to bring into play effect at double by rhPDGF and TGF-β use in conjunction.
Because there is epidermal barrier in healthy skin, make moisture content, oils and fats, electrolyte, protein macromolecule can't free in and out the horny layer of epidermis, make conditioning such as rhPDGF repair composition and can only see through the part that passages such as hair follicle and sweat gland enter the skin corium denier, influence has limited these active component and has played a role.The present invention passes through the little Jie's art of skin corium (pressure importing, micropin importing, frosted Exfoliating Scrub importing, microwave importing, radio frequency importing, electrophoresis import, spray importing, intradermal injection importing, the importing of pixel laser and applied in any combination thereof), can make the Transdermal absorption efficient of cell growth factor improve 10-1000 doubly, satisfy the needs of different skin beautifying and antisenility reparation conditioning.
Summary of the invention
The invention discloses a kind of be used to improve looks repair the preparation and the method for wrinkle of skin and depression defect, import maneuver or interventional medicine means by skin corium trace interventional procedure-utilization, overcome or penetrate epidermal barrier trace (Gamma Magnitude unit) is contained derivative growth factor of recombined human blood platelet (the recombinant human Platelet-Derived Growth Factor that bioactive ingredients has dermal tissue reparation function, be called for short rhPDGF), transforming growth factor (Transforming Growth Factor, be called for short TGF), hyaluronic acid (HA) compound formulation imports the target cell of skin corium target location, stimulate growth of fibroblasts and mitosis in the skin corium, collagen protein and hyaluronic synthetic and secretion, the generation of collagen fiber and elastic fiber and reparation, thereby permanent repair is such as skin depressions, deep wrinkle, skin defects such as corium fabric tissue fracture reach and beautify the antidotal effect of skin.
Skin corium trace interventional procedure can improve the absorption efficiency of skin corium cell growth factor compound formulation, the purpose of realization beautifying skin reparation deep layer wrinkle and skin defect specifically includes but not limited to pressure importing, micropin importing, the importing of frosted exfoliation, microwave radio importing, ultrasound wave importing, electrophoresis importing, injection importing, intradermal injection importing, laser importing and applied in any combination thereof.Pressure imports and to be meant by comprising that cell growth factor compound formulation that pressure that massage, beatings, inflation platen etc. are applied to the skin outside will be coated in skin surface is by hair follicle and sweat gland and the acceleration of the horny layer slit method through epidermal barrier.The micropin importing is meant and utilizes sewing single needle, sewing pricking with needle, micropin running roller to make tiny micropore in the epidermal surface of target site, forms introduction channel in short-term, promotes to be coated in the method for the cell growth factor compound formulation of skin surface by epidermal barrier.The importing of frosted exfoliation is meant and utilizes abrasive material suitably to polish at skin surface, removes the dead bark of horny layer outside and reduces cuticular thickness, promotes to be coated in the method for the cell growth factor compound formulation of skin surface by epidermal barrier.Microwave radio imports and to be meant and to utilize microwave/radio frequency to import instrument, improves the skin internal temperature with the frequency electromagnetic waves of 10KHz-100GHz, strengthens the gap between corneocyte, with the method for the horny layer permeability that strengthens the cell growth factor preparation.Ultrasound wave imports and to be meant the principle of utilizing the ultra sonic machinery vibration, strengthens the intercellular gap of keratodermatitis with the high speed of the 10KHz-1MHz ripple that shakes, with the method for the horny layer permeability that strengthens the cell growth factor preparation.Electrophoresis imports and is meant the electrostatic field force that produces by the electrophresis apparatus introducing equipment, causes cell membrane aquaporin (Aquaporins) open-minded, effectively bioactive material is infiltrated the method for skin corium cell by this passage.Spray importing and be meant that the superpower pressure by needleless injector is pressed into skin with medicinal liquid by superfine liquid bundle, about 0.25 second of injection persistent period, reach painless, noiseless, safety, the method that fast enters skin corium.Intradermal injection imports the method that the cell growth factor compound formulation is injected into skin corium position to be repaired by syringe that is meant.Laser imports and be meant that the micropore that utilizes pixel laser, dot matrix laser to form imports the cell growth factor compound formulation method of skin corium on horny layer.
Description of drawings
The specific embodiment
Active component: derivative growth factor of recombined human blood platelet rhPDGF-BB, source: Merck chemical, invitrogen; Transforming growth factor rhTGF-β, source: Merck chemical, invitrogen.
Functional component: hyaluronic acid HA, source: honest Fu Ruida (injection stage).
Auxiliary element: mannitol, dextran, water-solubility collagen, boric acid leucine, boric acid valine, boric acid alanine, N-acetylcystein, propylene glycol, Zuo En, source: commercially available (injection stage).
The preparation of freeze-dried powder preparation: active component, functional component and auxiliary element are mixed with aqueous solution with cryogenic purincation water, and place ice bath standby.Above-mentioned aqueous solution branch is installed in the clean cillin bottle,, place freeze dryer plug false add plug.Open the vacuum lyophilization agent and formulation temperature is reduced to-60 ℃, temperature fall time postpones 3-6 hour.Start vacuum pump, vacuum in the operating room is evacuated to 0.3MPa.Heat up gradually, distillation, the distillation time is 12-24 hour.Water sublimed to preparation finishes, and charges into nitrogen in the operating room, and the cap seal dress is rolled in tamponade.
The manufacturing of special-purpose lyase: with the PBS solution dissolving of auxiliary element with pH5.0-7.4, add glycerol, mix homogeneously according to dosage divided to install in the cillin bottle, jumps a queue, and rolls the cap seal dress, with 105 ℃ of steam high temperature sterilizes 20 minutes.
Embodiment 1
Measure rhPDGF 0.01g～0.1g, TGF 0.001g～0.1g, HA 1g～10g, mannitol 10～100g, dextran 10～100g, water-solubility collagen 5～50g, add purified water to 1000g, packing, lyophilizing charges into nitrogen, the cap seal dress is rolled in tamponade.Boric acid leucine 0g～10g, boric acid valine 0g～10g, boric acid alanine 0g～10g, N-acetylcystein 0g～10g, propylene glycol 0g～5g, assistant grace 0g～5g, pH5.0～7.5PBS add to 1000g, packing, roll cap seal dress, with 105 ℃ of steam high temperature sterilizes 20 minutes.
Embodiment 1 described cell growth factor compound formulation is applicable to that pressure importing, frosted Exfoliating Scrub importing, microwave radio import, ultrasound wave imports, electrophoresis imports and applied in any combination.
Embodiment 2
Measure rhPDGF 0.01g～0.1g, TGF 0.001g～0.1g, HA 5g～20g, mannitol 10～100g, add purified water to 1000g, packing, lyophilizing charges into nitrogen, and the cap seal dress is rolled in tamponade.Dextran 10～100g, water-solubility collagen 5～50g, pH5.0～7.5PBS added to 1000g, and cap seal dress is rolled in packing, with 105 ℃ of steam high temperature sterilizes 20 minutes.
Embodiment 2 described cell growth factor compound formulations are applicable to that micropin imports, the frosted Exfoliating Scrub imports, injection imports, intradermal injection imports, laser imports and applied in any combination.
Embodiment 3
Measure rhPDGF 0.01g～0.1g, TGF 0.001g～0.1g, HA 1g～10g, carbomer 3～10g, carboxymethyl cellulose 5～15g, dextran 10～100g, water-solubility collagen 5～50g, boric acid leucine 0g～10g, boric acid valine 0g～10g, boric acid alanine 0g～10g, N-acetylcystein 0g～10g, propylene glycol 0g～5g, assistant grace 0g～5g, nipalgin fat 0.01～0.5g, add purified water to 1000g, packing, roll the cap seal dress, deepfreeze.
Embodiment 3 described cell growth factor compound formulations are applicable to that pressure importing, frosted Exfoliating Scrub importing, microwave radio import, ultrasound wave imports, electrophoresis imports and applied in any combination.
Quality evaluation: above-mentioned three embodiment carry out safety testing, stability test and beauty functions test, the result shows cell growth factor compound formulation safety and stability of the present invention, has clear and definite beautifying and antisenility wrinkle removal effect, be suitable as cosmetics and cooperate the little Jie's art of beauty treatment to practice, have a good application prospect.
Safety testing
| Irritated test | Test case | Non-stimulated | Slight irritated | Other untoward reaction | Safety evaluatio |
| Embodiment 1 | ??120 | ??119 | ??1 | ??0 | Good |
| Embodiment 2 | ??120 | ??120 | ??0 | ??0 | Good |
| Embodiment 3 | ??120 | ??118 | ??2 | ??0 | Good |
Stability test
| Active conservation rate | Test case | 6 months | 12 months | 18 months | 24 months | Estimation of stability |
| Embodiment 1 | ??10 | ?99 | ??98 | ??96 | ??95 | Good |
| Embodiment 2 | ??10 | ?99 | ??98 | ??96 | ??95 | Good |
| Embodiment 3 | ??10 | ?95 | ??90 | ??86 | ??80 | Well |
The cosmetic result test
| Crinkle-removing whitening | Test case | Highly significant | Significantly | Improve | To no effect | Estimate |
| Embodiment 1 | ??90 | ??19 | ??25 | ??40 | ??6 | Well |
| Embodiment 2 | ??50 | ??30 | ??20 | ??0 | ??0 | Good |
[0038]
| Embodiment 3 | ??150 | ??31 | ??37 | ??61 | ??21 | Well |
Claims (7)
1. cell growth factor compound formulation and be used for the method that beautifying skin is repaired deep layer wrinkle and skin defect.
2. the described cell growth factor compound formulation of claim 1 includes but not limited to derivative growth factor of recombined human blood platelet (recombinanthuman Platelet-Derived Growth Factor, abbreviation rhPDGF), recombined human transforming growth factor (recombinant humanTransforming Growth Factor, rhTGF), hyaluronic acid (Hyaluronic Acid is called for short HA).
3. the described method of claim 1 refers to be meant by importing maneuver or interventional medicine means by corium trace interventional procedure, trace (Gamma Magnitude unit) is contained the growth factor activity composition have target cell and the target tissue that dermal tissue is repaired rhPDGF, the TGF of function, hyaluronic acid compound formulation importing skin corium target location, beauty treatment reparation and anti-ageing reduce wrinkle effect that the performance of cell growth factor compound formulation is determined.The micro-interventional procedure of skin corium includes but not limited to pressure importing, micropin importing, the importing of frosted exfoliation, microwave radio importing, ultrasound wave importing, electrophoresis importing, injection importing, intradermal injection importing, laser importing and applied in any combination thereof.
4. the described cell growth factor compound formulation of claim 2 rhPDGF-BB content 10ng/g-1mg/g does not wherein comprise rhPDGF-AB, rhPDGF-AA, and molecular weight is 23～28KDa, and purity is not less than 95%, and specific activity is not less than 1 * 10
5IU/mg, level of endotoxin are lower than 0.1ng/ μ g, and preferred purity is higher than 99%, and specific activity is higher than 5 * 10
5The rhPDGF-BB of IU/mg.
5. the described cell growth factor compound formulation of claim 2 TGF-β content 0ng/g-100 μ g/g does not wherein wherein comprise TGF-α, and molecular weight is 25KDa, and purity is not less than 95%, and level of endotoxin is lower than 0.1ng/ μ g.
6. the described cell growth factor compound formulation of claim 2 hyaluronic acid 1 μ g/g-50mg/g wherein.
7. the described pressure of claim 3 imports and is meant by comprising that cell growth factor compound formulation that pressure that massage, beatings, inflation platen etc. are applied to the skin outside will be coated in skin surface pressurizes through epidermal barrier by hair follicle and sweat gland and horny layer slit, improve the absorption efficiency of skin corium cell growth factor compound formulation, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described micropin importing of claim 3 is meant and utilizes sewing single needle, sewing pricking with needle, micropin running roller to make tiny micropore in the epidermal surface of target site, form introduction channel in short-term, promote the cell growth factor compound formulation that is coated in skin surface to pass through epidermal barrier, improve the absorption efficiency of skin corium cell growth factor compound formulation, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described frosted exfoliation importing of claim 3 is meant and utilizes abrasive material suitably to polish at skin surface, remove the dead bark of horny layer outside and reduce cuticular thickness, promote the cell growth factor compound formulation that is coated in skin surface to pass through epidermal barrier, improve the absorption efficiency of skin corium cell growth factor compound formulation, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described microwave radio importing of claim 3 is meant and utilizes microwave/radio frequency to import instrument, frequency electromagnetic waves with 10KHz-100GHz improves the skin internal temperature, strengthen the gap between corneocyte, to strengthen the horny layer permeability of cell growth factor preparation, improve the absorption efficiency of skin corium cell growth factor compound formulation, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described ultrasound wave of claim 3 imports and is meant the principle of utilizing the ultra sonic machinery vibration, strengthen the intercellular gap of keratodermatitis with the high speed of the 10KHz-1MHz ripple that shakes, to strengthen the horny layer permeability of cell growth factor preparation, improve the absorption efficiency of skin corium cell growth factor compound formulation, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described electrophoresis of claim 3 imports and is meant the electrostatic field force that produces by the electrophresis apparatus introducing equipment, cause cell membrane aquaporin (Aquaporins) open-minded, effectively bioactive material is infiltrated cell by this passage, realize the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described injection of claim 3 imports and is meant that the superpower pressure by needleless injector is pressed into skin with medicinal liquid by superfine liquid bundle, about 0.25 second of injection persistent period, reaches painless, noiseless, safety, quick, the purpose that the realization beautifying skin is repaired deep layer wrinkle and skin defect.The described intradermal injection importing of claim 3 is meant by syringe the cell growth factor compound formulation is injected into skin corium position to be repaired, realizes the purpose of beautifying skin reparation deep layer wrinkle and skin defect.The described laser of claim 3 imports and be meant that the micropore that utilizes pixel laser, dot matrix laser to form imports skin corium with the cell growth factor compound formulation, realizes the purpose of beautifying skin reparation deep layer wrinkle and skin defect on horny layer.
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