CN102008754A - Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration - Google Patents
Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration Download PDFInfo
- Publication number
- CN102008754A CN102008754A CN2010105867931A CN201010586793A CN102008754A CN 102008754 A CN102008754 A CN 102008754A CN 2010105867931 A CN2010105867931 A CN 2010105867931A CN 201010586793 A CN201010586793 A CN 201010586793A CN 102008754 A CN102008754 A CN 102008754A
- Authority
- CN
- China
- Prior art keywords
- cell growth
- growth factor
- hyaluronic acid
- nutrient
- acid derivatives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration. The cosmetic filler is prepared from the following components by weight percent: 1-20% of cell growth factor, 5-80% of cell growth nutrient and 0.1-20% of hyaluronic acid gel; and the preparation method of the cosmetic filler comprises: fully dissolving the cell growth factor and the cell growth nutrient in water; adding the hyaluronic acid gel powder, and fully stirring to enable the cell growth factor and the cell growth nutrient to be absorbed by crosslinked hyaluronic acid gel. In the invention, the hyaluronic acid gel and the growth factor are compounded for filling defected soft tissues, thus the purposes of slowly releasing the growth factor to promote cell growth and repairing the defected soft tissues are achieved, and the effectiveness is durable; and extensive trials show that the cosmetic filler can become effective as soon as the cosmetic filler is injected, the handfeel is soft and real, rejection is avoided, red and swollen phenomena are prevented in the injection process, the growth effect is favorable, and the defect of short retention time caused by only injecting hyaluronic acid gel is solved.
Description
Technical field
The present invention relates to the regenerated filler of a kind of promotion human body soft tissue, especially render a service beauty treatment filler lasting, that have the promotion soft tissue regeneration of long-term efficacy.
Background information
Tissue often influences face appearance etc. because the various pains of injury, disease, contingency, wound etc. cause the default or facial wound of soft tissue, need fill and repair or beauty treatment etc., to repair default soft tissue or to make people's face well-done, generous or increase beautiful sense etc. by simple cosmetic surgery; In the prior art, as the filler in the implant into body of reparation or cosmetic surgery, normally used is paraffin, silica gel, animal collagen, polyacrylamide gel etc., rejection takes place because of the material that is not human body self in these materials easily, some material even produce the stronger non-constant of rejection, histocompatibility with human body, cause a lot of side effect, its scope of application is subjected to bigger restriction.
In the prior art, hyaluronic acid derivatives is a kind of good soft tissue filler, is the important component part of tissue, and histocompatibility is very good, as animal collagen rejection might not arranged.But directly use single hyaluronic acid derivatives as soft tissue filling material, will decompose absorption because of metabolism after a while, do not reach the purpose of long-term filling.
In the prior art, promote the cell growth factor of cell growth, has the regenerated function of the histiocyte of promotion, but the reproduction speed of cell and cell are subjected to the time length of somatomedin effect that obvious relation is arranged, and that is to say that cell only just can ceaselessly grow under the stimulation that is subjected to cytokine for a long time, however the direct injection cell growth factor, at suitable of short duration of the time that body local stops, what have has only 1-2 hour, does not reach the purpose of stimulating cellular growth, and its effectiveness is difficult to lastingly.
Goal of the invention
The present invention is directed in the prior art as repairing or the existing above-mentioned deficiency of the intravital filler of implant into body of cosmetic surgery, a kind of beauty treatment filler with promotion soft tissue regeneration of long-term efficacy is provided.
Technical scheme of the present invention: have the beauty treatment filler of the promotion soft tissue regeneration of long-term efficacy, it is characterized in that comprising following components in weight percentage,
Promote the cell growth factor of cell growth, content is 0.1-20%;
Nutrient for cell growth, content are 5-80%;
Hyaluronic acid derivatives, content are 0.1-20%;
Above-mentioned cell growth factor, nutrient for cell growth are pressed aforesaid part by weight, fully be dissolved in the water, add the hyaluronic acid derivatives powder then, fully stir, make cell growth factor and nutrient for cell growth in the solution be crosslinked the absorption of hyaluronic acid derivatives powder.
Further feature is: also comprise the human body self tissue stem cell, addition accounts for the 1-20% of gross weight.
After the adding hyaluronic acid derivatives fully stirs, add catalyst, the factor and the hyaluronic acid of cell growth are reacted, the somatomedin joint is propped up to hyaluronic acid.
The present invention has the beauty treatment filler of the promotion soft tissue regeneration of long-term efficacy, is equivalent to prior art, has following advantage:
1, histocompatibility is very good, rejection can not take place basically; The present invention is grouped together hyaluronic acid derivatives and cell growth factor and the cell needed nutrient of growing, and the histocompatibility of hyaluronic acid derivatives and human body is very good, rejection can not take place.
2, the effectiveness persistency is strong, has secular effectiveness; Hyaluronic acid derivatives plays interim filling and supports
Effect, simultaneously somatomedin and nutrient for cell growth there is slow releasing function, make the hyaluronic acid derivatives border cell be subjected to the effect of cell growth factor for a long time and grow, when hyaluronic acid when slowly metabolism absorbs in vivo, somatomedin and nutrient for cell growth be slow release also, has just promoted border cell's growth, so how many how many cells of cross-linked-hyaluronic acid metabolism just grow, just in time reach balance, so just reach the default purpose of long-term repair soft tissue, just have long-term efficacy.
3, in addition, the present invention can also be put prior cultured human body self tissue stem cell such as fibroblast, the preceding cell of fat etc. as accelerate tissue growth in this filler, these cells have the merisis ability, they can utilize in the gel and the surrounding tissue nutritional labeling, and growth is rapider under the effect of somatomedin.
4, filler of the present invention, be a kind ofly can be transplanted to the intravital promotion soft tissue regeneration of people, the comparatively ideal filler of effect, be widely used in the filling skin depressions, tissue defect also can be used for human body beauty treatment as wrinkle removing, rich, augmentation rhinoplasty, chin lengthening, breast enlargement etc.
5, through extensively on probation, injection takes effect immediately, and is soft true, not only do not have rejection, also do not have red and swollen phenomenon during injection, and growth result is good, overcome short defective of simple injection hyaluronic acid derivatives retention time.
Summary of the invention
Have the beauty treatment filler of the promotion soft tissue regeneration of long-term efficacy, it is characterized in that comprising following components in weight percentage,
Promote the cell growth factor of cell growth, content is 0.1-20%;
Nutrient for cell growth, content are 5-80%;
Hyaluronic acid derivatives, content are 0.1-20%;
The cell growth factor of described promotion cell growth, mainly be meant fibroblast growth factor, epidermal growth factor, platelet class somatomedin, insulin like growth factor, transforming growth factor, nerve growth factor, calf blood protein-removed extraction etc. include but not limited to above-mentioned concrete cell growth factor; Can be singly a kind of with wherein, also can mix with arbitrary proportion and the cell growth factor of the multiple proportioning that obtains with wherein two or more; According to different position of human body and different treatment, beauty treatment needs, select suitable a kind of cell growth factor for best usually, also can adopt the various kinds of cell somatomedin to mix and the mixed type cell growth factor that obtains with arbitrary proportion.
Nutrient for cell growth mainly is meant vitamin, trace element, electrolyte, aminoacid, glucose, organic acid etc., includes but not limited to above-mentioned concrete nutrient or nutrition goods and materials; Nutrient for cell growth has had the product of plurality of specifications to sell on market as a kind of material, can be classified as prior art, and the proportioning of employing prior art is mixed and obtained.
Hyaluronic acid derivatives plays slow release, filling, support as slow releasing agent.
Explained hereafter method of the present invention is: cell growth factor, the nutrient for cell growth of above-mentioned promotion cell growth are pressed aforesaid part by weight, fully be dissolved in the water, need stirring or elevated temperature etc. in case of necessity, make it to reach the requirement that promotes the cell growth, add the cross-linked hyaluronic acid gel powder then, fully stir, make cell growth factor and nutrient for cell growth in the solution be crosslinked the absorption of hyaluronic acid derivatives powder fully, and can not separate out moisture.
Also comprise the human body self tissue stem cell,,, add in this filler,, play the effect of accelerate tissue growth as supplementary element as cell or other stem cell before fibroblast, the fat with prior cultured human body self tissue stem cell.The addition of human body self tissue stem cell accounts for the 1-20% of gross weight.
Can fully stir at adding hyaluronic acid derivatives solution, and behind the mix homogeneously, adding weight is that the 0.005-1% aldehyde material is as catalyst, what this aldehyde material was commonly used is formaldehyde, Biformyl, glutaraldehyde etc., but be not limited to these three kinds, the factor and the hyaluronic acid derivatives of cell growth are reacted, the somatomedin joint is propped up to hyaluronic acid, reuse weight is that the cross-linking agent of 0.1-20% makes cross-linking hyaluronic acid form gel, what cross-linking agent was commonly used is divinylsulfone, also can use hydrazides, 1,4 butanediol glycidyl ethers, carbodiimide etc., but be not limited to that these are several.
Cell growth factor of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Nutrient for cell growth of the present invention, its content can be 5%, 6%, 8%, 10%, 15%, 20%, 28%, 30%, 35%, 40%, 45%, 48%, 50%, 54%, 58%, 60%, 65%, 68%, 70%, 75%, 78%, 80% etc.; Hyaluronic acid derivatives of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Human body self tissue stem cell of the present invention, its content can be 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc.; Cross-linking agent of the present invention, its content can be 0.1%, 0.2%, 0.5%, 0.8%, 1%, 2%, 5%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20% etc., can both satisfy needs of the present invention.
Embodiment 1: with cell growth factor and nutrient for cell growth by a certain percentage of the weight, cell growth factor is 40 grams, nutrient for cell growth 1 standard bag (the DMEM dehydrated medium is used to prepare 1000 ml vols), fully be dissolved in 1000 ml waters and (meet medical distilled water or pure water etc.), need stirring or elevated temperature etc. in case of necessity, add 20 gram hyaluronic acid derivatives powder then, fully stir and soak, make cell growth factor and nutrient for cell growth in the solution be crosslinked the hyaluronic acid derivatives absorption fully, after no longer separating out moisture, make filler of the present invention, sterilization is 30 minutes under 100 degree.
Embodiment 2: with cell growth factor and nutrient for cell growth by a certain percentage of the weight, cell growth factor is 45 grams, nutrient for cell growth 1 standard bag (the DMEM dehydrated medium is used to prepare 1000 ml vols), fully be dissolved in 1000 ml waters and (meet medical distilled water or pure water etc.), need stirring or elevated temperature etc. in case of necessity, make it to reach the requirement that promotes the cell growth, add 25 gram hyaluronic acids then, fully add 0.005% formaldehyde behind the mixing, the factor and the hyaluronic acid of cell growth are reacted, the cell growth factor joint is propped up to hyaluronic acid, add 50 divinylsulfones then and form gel, sterilization is 30 minutes under 100 degree.
Embodiment 3:, the filler that will make according to the inventive method production, under aseptic condition, implant and cultivate successful human body self tissue stem cell in advance, as (including but not limited to these cells) such as cells before fibroblast, the fat, the filler that will implant the human body self tissue stem cell then is expelled to the place that human body needs.
Using method: the filler of the present invention that will make is put into syringe, and the syringe needle with different length injects the default position of soft tissue as required, makes local form reach ideal degree; Be expelled to the default position of human body soft tissue, play conduct or the beautifying face effect of repairing.
Claims (3)
1. have the beauty treatment filler of the promotion soft tissue regeneration of long-term efficacy, it is characterized in that comprising following components in weight percentage,
Promote the cell growth factor of cell growth, content is 0.1-20%;
Nutrient for cell growth, content are 5-80%;
Hyaluronic acid derivatives, content are 0.1-20%;
Described cell growth factor includes but not limited to fibroblast growth factor, epidermal growth factor, platelet class somatomedin, insulin like growth factor, transforming growth factor, nerve growth factor, calf blood protein-removed extraction; Single a kind of with wherein, or with wherein two or more with the arbitrary proportion mixing and obtain;
Above-mentioned cell growth factor, nutrient for cell growth are pressed aforesaid part by weight, fully be dissolved in the water, add the hyaluronic acid derivatives powder then, fully stir, make cell growth factor and nutrient for cell growth in the solution be crosslinked the absorption of hyaluronic acid derivatives powder.
2. described beauty treatment with promotion soft tissue regeneration of long-term efficacy is filled according to claim 1
Agent is characterized in that: also comprise the human body self tissue stem cell, addition accounts for the 1-20% of gross weight.
3. according to claim 1 or 2 described beauty treatment filleies with promotion soft tissue regeneration of long-term efficacy, it is characterized in that: after the adding hyaluronic acid derivatives fully stirs, adding weight is that the 0.005-1% aldehyde material is as catalyst, the factor and the hyaluronic acid derivatives of cell growth are reacted, the somatomedin joint is propped up to hyaluronic acid; Reuse weight is that the cross-linking agent of 0.1-20% makes cross-linking hyaluronic acid form gel, and what cross-linking agent was commonly used is divinylsulfone, or hydrazides, or 1,4 butanediol glycidyl ethers, or carbodiimide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010586793.1A CN102008754B (en) | 2010-12-14 | 2010-12-14 | Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010586793.1A CN102008754B (en) | 2010-12-14 | 2010-12-14 | Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102008754A true CN102008754A (en) | 2011-04-13 |
| CN102008754B CN102008754B (en) | 2014-01-15 |
Family
ID=43839205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010586793.1A Active CN102008754B (en) | 2010-12-14 | 2010-12-14 | Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102008754B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805880A (en) * | 2011-05-31 | 2012-12-05 | 上海国睿生命科技有限公司 | Tissue engineering gel scaffold material, preparation method and use thereof |
| CN102813961A (en) * | 2011-06-10 | 2012-12-12 | 冯淑芹 | Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof |
| CN104014000A (en) * | 2014-06-10 | 2014-09-03 | 白晋 | Injection reagent for cosmetic filling |
| CN105030647A (en) * | 2015-09-14 | 2015-11-11 | 广州赛莱拉干细胞科技股份有限公司 | Preparation reducing wrinkles and preparing method thereof |
| CN105106093A (en) * | 2015-09-14 | 2015-12-02 | 广州赛莱拉干细胞科技股份有限公司 | Facial rejuvenation face beautifying preparation and preparation method thereof |
| CN105233270A (en) * | 2015-11-12 | 2016-01-13 | 上海昊海生物科技股份有限公司 | Medicine composition for water-optical treatment and application of medicine composition |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN112386519A (en) * | 2019-08-16 | 2021-02-23 | 上海可米日化股份有限公司 | Beauty gel and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785852A (en) * | 2010-01-21 | 2010-07-28 | 孙杰 | Dermis trace intervention anti-wrinkle cosmetic preparation and method |
-
2010
- 2010-12-14 CN CN201010586793.1A patent/CN102008754B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785852A (en) * | 2010-01-21 | 2010-07-28 | 孙杰 | Dermis trace intervention anti-wrinkle cosmetic preparation and method |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805880A (en) * | 2011-05-31 | 2012-12-05 | 上海国睿生命科技有限公司 | Tissue engineering gel scaffold material, preparation method and use thereof |
| CN102813961A (en) * | 2011-06-10 | 2012-12-12 | 冯淑芹 | Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof |
| CN104014000A (en) * | 2014-06-10 | 2014-09-03 | 白晋 | Injection reagent for cosmetic filling |
| CN105030647A (en) * | 2015-09-14 | 2015-11-11 | 广州赛莱拉干细胞科技股份有限公司 | Preparation reducing wrinkles and preparing method thereof |
| CN105106093A (en) * | 2015-09-14 | 2015-12-02 | 广州赛莱拉干细胞科技股份有限公司 | Facial rejuvenation face beautifying preparation and preparation method thereof |
| CN105106093B (en) * | 2015-09-14 | 2018-06-15 | 广州赛莱拉干细胞科技股份有限公司 | A kind of face rejuvenation cosmetic formulation and preparation method thereof |
| CN105233270A (en) * | 2015-11-12 | 2016-01-13 | 上海昊海生物科技股份有限公司 | Medicine composition for water-optical treatment and application of medicine composition |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN106421920B (en) * | 2016-11-16 | 2019-05-14 | 沈阳细胞治疗工程技术研发中心有限公司 | A kind of fat filler and preparation method thereof |
| CN112386519A (en) * | 2019-08-16 | 2021-02-23 | 上海可米日化股份有限公司 | Beauty gel and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102008754B (en) | 2014-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102008754B (en) | Cosmetic filler with long-term effectiveness capable of promoting soft tissue regeneration | |
| AU2013251800B2 (en) | Flowable tissue matrices | |
| CN105311677B (en) | Method, substrate and the system of cell inoculation for medical implants | |
| JP2021164731A (en) | Injectable silk fibroin foams and uses thereof | |
| Young et al. | Injectable biomaterials for adipose tissue engineering | |
| Zhao et al. | Osteogenic media and rhBMP-2-induced differentiation of umbilical cord mesenchymal stem cells encapsulated in alginate microbeads and integrated in an injectable calcium phosphate-chitosan fibrous scaffold | |
| CN106999635A (en) | Repair of cartilage graft support and its manufacture method | |
| EP3545979A1 (en) | Compositionsand methods for tissue filling and regeneration | |
| CN105999410A (en) | Acellular tissue matrix composite and preparation method thereof | |
| ES2739656T3 (en) | Tissue adhesive in which collagen and fibrin are mixed, and method to prepare it | |
| CN102657584A (en) | Degradation-stabilised, biocompatible collagen matrices | |
| JP2013500315A (en) | Injectable composition combining filler and fibroblast growth medium | |
| JP2009542384A5 (en) | ||
| CN106176292A (en) | A kind of biodegradable injection molding process decorative material and preparation method thereof | |
| CN105056285B (en) | It is a kind of can adhesion organization crack growth factor combine dressing and preparation method thereof | |
| CN107921101A (en) | For generating the powder composition and its application method of cross-linked proteins foam | |
| CN101610791A (en) | The compositions of promoting bone regeneration and recovery | |
| CN104587531B (en) | A kind of preparation method of gel stent of repairing articular cartilage damage | |
| CN115429937B (en) | Soft tissue filling repair material and preparation method thereof | |
| CN108498855A (en) | A kind of antibacterial anti hemorrhagic colloidal sol and preparation method thereof | |
| CN108465124A (en) | A kind of self-crosslinking injection beauty packing material and preparation method | |
| CN101347638A (en) | Tissue-patching material for injection and preparation method thereof | |
| CN109337383B (en) | Collagen-based self-repairing hydrogel and preparation method thereof | |
| CN106110377B (en) | A kind of bioadhesive and its preparation method and application based on epsilon-polylysine | |
| CN108578617A (en) | A kind of application of umbilical cord blood mesenchymal stem cell drug in promoting knee cartilage regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |