Summary of the invention
The object of the invention and design philosophy are just to existing weak point in the above-mentioned prior art; Nutritional needs characteristics according to the different growth phases of yam; Simultaneously according to the spray culture characteristics; A kind of potato spray culture nutrient solution that has comprised N, P, K element and multiple type of trace element and preparation method thereof is provided, through adding sterilant, controls the generation of main disease in the spray culture process effectively simultaneously.In nutritive medium supply, realize the dynamic management of nutritive medium, promptly, solve plant poor growth in seedling stage in the potato spray culture according to the corresponding nutritive ingredient of different increases in demand of yam seedling stage, knot potato phase, knot potato phase over growth influences problems such as output.
The object of the invention can be realized through following technique measures:
Potato spray culture nutrient solution of the present invention comprises mother liquor A, mother liquor B, mother solution C, mother liquor D and mother liquor E; Wherein said mother liquor A comprises the raw material of following weight volume ratio: saltpetre 30-40g/L; Potassium primary phosphate 20-30g/L; MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L, three water Sytron 4.0-5.0g/L, surplus is a water; Said mother liquor B comprises the raw material of following weight volume ratio: four water-calcium nitrate 45-55g/L, and surplus is a water; Said mother solution C comprises the raw material of following weight volume ratio: potassiumiodide 0.8-0.9g/L; Boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L, Zinc Sulphate Heptahydrate 8.0-9.0g/L; Sodium Molybdate Dihydrate 0.2-0.3g/L; CoCL2 0.025-0.03g/L, cupric sulfate pentahydrate 0.02-0.03g/L, surplus is a water; Said mother liquor D comprises the raw material of following weight volume ratio: urea 100-200g/L, and surplus is a water; Said mother liquor E comprises the raw material of following weight volume ratio: potassium primary phosphate 100-200g/L, surplus is a water.
The method that is used to prepare said potato spray culture nutrient solution of the present invention comprises the steps:
A, preparation mother liquor A: volume ratio is got saltpetre 30-40g/L by weight, potassium primary phosphate 20-30g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L, three water Sytron 4.0-5.0g/L,, the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 45-55g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.8-0.9g/L by weight, boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L; Zinc Sulphate Heptahydrate 8.0-9.0g/L, Sodium Molybdate Dihydrate 0.2-0.3g/L, CoCL2 0.025-0.03g/L; Cupric sulfate pentahydrate 0.02-0.03g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 100-200mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 100-200mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Beneficial effect of the present invention is following:
1, owing to N, P, K element proportioning in this prescription relatively meet the needs that the potato raw long hair is educated, so the output of spray culture is improved largely.
2, owing to added agricultural streptomycin, can reduce the mashed seedling of plant, seedling death phenomenon, effectively improve the surviving rate of plant at yam field planting initial stage and seedling phase.Knot adds metalaxyl the potato phase in nutritive medium, can effectively prevent disease such as late blight through the nutritive medium circulation propagate maybe, thereby improve the output of spray culture and the quality of micro potato.
3, because this prescription adds a certain proportion of urea in the potato seedling phase, improved the ratio of nitrogenous fertilizer in the nutritive medium, can promote the cauline leaf ramp, thereby the knot potato joint position in the groove is increased fast, for potato spray culture has been laid the high yield basis.
4, phosphate fertilizer and potash fertilizer can promote yam knot potato and potato piece to expand, this prescription adds a certain proportion of potassium primary phosphate tying the potato phase, have replenished the deficiency of potash fertilizer and phosphate fertilizer in the nutritive medium, can improve the output of potato spray culture greatly.
Through evidence; In potato spray culture, use nutrient solution prescription individual plant knot potato number of the present invention to reach 47.3; Expand into the potato number and reached 87.3; Improved 19.7% and 26.0% than MS culture medium prescription respectively, improved 13.7% and 10.2%, wherein expanded into the potato number and expand into several 76 of potato than domestic relevant report individual plant and improved 14.9% than the disclosed nutrient solution prescription of Chinese patent ZL98115898.6.
This shows; The present invention is through the proportioning of adjustment N, P, K element; Add sterilant such as agricultural streptomycin, metalaxyl and carry out the nutritive medium dynamic management; Improve the knot potato quantity and the potato piece size of potato spray culture greatly, and made full use of each effective constituent in the nutritive medium, reduced to produce and dropped into.
Embodiment
To combine embodiment to further describe below the present invention:
Embodiment 1
A, preparation mother liquor A: volume ratio is got saltpetre 39g/L by weight, potassium primary phosphate 24g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 37g/L, three water Sytron 4.9g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 47g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.83g/L by weight, boric acid 6.2g/L, four water manganous sulfate 22.3g/L; Zinc Sulphate Heptahydrate 8.6g/L, Sodium Molybdate Dihydrate 0.25g/L, CoCL2 0.025g/L; Cupric sulfate pentahydrate 0.025g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 100g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 100g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 100mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 100mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Embodiment 2
A, preparation mother liquor A: volume ratio is got saltpetre 32g/L by weight, potassium primary phosphate 27g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 39g/L, three water Sytron 4.2g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 52g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.89g/L by weight, boric acid 6.7g/L, four water manganous sulfate 22.7g/L; Zinc Sulphate Heptahydrate 8.3g/L, Sodium Molybdate Dihydrate 0.27g/L, CoCL2 0.028g/L; Cupric sulfate pentahydrate 0.022g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 150g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 150g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 200mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 200mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Embodiment 3
A, preparation mother liquor A: volume ratio is got saltpetre 36g/L by weight, potassium primary phosphate 22g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35g/L, three water Sytron 4.6g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 54g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.86g/L by weight, boric acid 6.5g/L, four water manganous sulfate 22.5g/L; Zinc Sulphate Heptahydrate 8.9g/L, Sodium Molybdate Dihydrate 0.22g/L, CoCL2 0.03g/L; Cupric sulfate pentahydrate 0.029g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 180g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 180g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 150mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 150mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.