CN101781148B - Potato spray culture nutrient solution and preparation method thereof - Google Patents

Potato spray culture nutrient solution and preparation method thereof Download PDF

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CN101781148B
CN101781148B CN201010106179A CN201010106179A CN101781148B CN 101781148 B CN101781148 B CN 101781148B CN 201010106179 A CN201010106179 A CN 201010106179A CN 201010106179 A CN201010106179 A CN 201010106179A CN 101781148 B CN101781148 B CN 101781148B
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mother liquor
volume ratio
water
mother
preparation
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CN101781148A (en
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庞淑敏
方贯娜
李建欣
周建华
赵建设
李红记
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Abstract

The invention discloses potato spray culture nutrient solution and a preparation method thereof. The potato spray culture nutrient solution comprises mother solution A, mother solution B, mother solution C, mother solution D and mother solution E. The mother solution A comprises the following raw materials in weight volume ratio: 30 to 40 g/L of potassium nitrate, 20 to 30 g/L of monopotassium phosphate, 4.0 to 5.0 g/L of ferrisodium ethylenediamine tetracetate trihydrate, 3.0 to 4.0 g/L of sodium ethylenediamine tetracetate dehydrate, and the balance of water. The mother solution B comprises the following raw materials in weight volume ratio: 45 to 55 g/L of calcium nitrate tetrahydrate, and the balance of water. The mother solution C comprises the following raw materials in weight volume ratio: 0.8 to 0.9 g/L of potassium iodide, 6.0 to 7.0 g/L of boric acid, 22 to 23 g/L of manganese sulfate tetrahydrate, 8.0 to 9.0 g/L of zinc sulfate heptahydrate, 0.2 to 0.3 g/L of sodium molybdate dehydrate, 0.025 to 0.03 g/L of cobalt chloride hexahydrate, 0.02 to 0.03 g/L of copper sulfate pentahydrate, and the balance of water. The mother solution D comprises the following raw materials in weight volume ratio: 100 to 200 g/L of urea, and the balance of water. The mother solution E comprises the following raw materials in weight volume ratio: 100 to 200 g/L of monopotassium phosphate, and the balance of water.

Description

Potato spray culture nutrient solution and preparation method thereof
Technical field
The present invention relates to all kinds of nutrition supply technology in the plant soil-less culturing, take into account the disease control technology simultaneously, relate to a kind of potato spray culture nutrient solution of producing micro potato for potato spray culture and preparation method thereof specifically.
Background technology
Yam is the fifth-largest crop in the world, and China is potato planting first big country.Because yam is an asexually propagated crop; Plantation throughout the year can accumulate virus; Thereby cause the seed potato serious degradation; Therefore, cultivating detoxification technology and soilless culture toxicity micro potato raising technology with stem-tip tissue is that the virus-free seed potato raising technology of core is being brought into play important effect in Potato Industry.Matrix-less potato cultivating is a kind of new micro potato production technology that grew up in recent years; It is with the root system of regularly supplying with plant behind the nutrient liquid atomizing through the nutritive medium recycle system (comprising liquid storing pool, inlet channel, water return pipeline and shower nozzle); Thereby for plant strain growth provides nutrition, and total system can realize robotization through controlling box.This technological advantage mainly is that micro potato output is high, is tens times that micro potato is produced in substrate culture; Secondly owing to can realize gathering and avoided infecting of various germs in the soil in batches; The quality of micro potato has also obtained very big improvement with kind property; Simultaneously owing to breed the raising of efficient and the robotization of production process; The production cost of micro potato is reduced significantly, and realized batch production and management automation that micro potato is produced.Comprehensive above advantage, potato spray culture comes into one's own gradually in toxicity-removing white potato potato seed breeding of method and brings into play the effect of wanting emphatically.
The sport technique segment that influences the spray culture effect is many, and wherein nutrient solution prescription is to influence one of key of success factor.Chinese patent ZL98115898.6 discloses a kind of matrix-less potato cultivating method and equipment, comprising nutrient solution prescription.The disclosed nutrient solution prescription of this patented technology has comprised the yam required various nutritive ingredients of growing, and spray culture is had certain effect of increasing production.But the proportioning of N, P, K element is not the yam needed optimum proportion that grows in this prescription, and this prescription is also inhaled fertile rule different features according to different yams in the period of growing simultaneously, and nutrient composition is carried out dynamic management.More than the deficiency of two aspects make the output of potato spray culture not bring into play to greatest extent, also wasted nutritive medium simultaneously to a certain extent, increased to produce and dropped into.
Summary of the invention
The object of the invention and design philosophy are just to existing weak point in the above-mentioned prior art; Nutritional needs characteristics according to the different growth phases of yam; Simultaneously according to the spray culture characteristics; A kind of potato spray culture nutrient solution that has comprised N, P, K element and multiple type of trace element and preparation method thereof is provided, through adding sterilant, controls the generation of main disease in the spray culture process effectively simultaneously.In nutritive medium supply, realize the dynamic management of nutritive medium, promptly, solve plant poor growth in seedling stage in the potato spray culture according to the corresponding nutritive ingredient of different increases in demand of yam seedling stage, knot potato phase, knot potato phase over growth influences problems such as output.
The object of the invention can be realized through following technique measures:
Potato spray culture nutrient solution of the present invention comprises mother liquor A, mother liquor B, mother solution C, mother liquor D and mother liquor E; Wherein said mother liquor A comprises the raw material of following weight volume ratio: saltpetre 30-40g/L; Potassium primary phosphate 20-30g/L; MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L, three water Sytron 4.0-5.0g/L, surplus is a water; Said mother liquor B comprises the raw material of following weight volume ratio: four water-calcium nitrate 45-55g/L, and surplus is a water; Said mother solution C comprises the raw material of following weight volume ratio: potassiumiodide 0.8-0.9g/L; Boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L, Zinc Sulphate Heptahydrate 8.0-9.0g/L; Sodium Molybdate Dihydrate 0.2-0.3g/L; CoCL2 0.025-0.03g/L, cupric sulfate pentahydrate 0.02-0.03g/L, surplus is a water; Said mother liquor D comprises the raw material of following weight volume ratio: urea 100-200g/L, and surplus is a water; Said mother liquor E comprises the raw material of following weight volume ratio: potassium primary phosphate 100-200g/L, surplus is a water.
The method that is used to prepare said potato spray culture nutrient solution of the present invention comprises the steps:
A, preparation mother liquor A: volume ratio is got saltpetre 30-40g/L by weight, potassium primary phosphate 20-30g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L, three water Sytron 4.0-5.0g/L,, the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 45-55g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.8-0.9g/L by weight, boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L; Zinc Sulphate Heptahydrate 8.0-9.0g/L, Sodium Molybdate Dihydrate 0.2-0.3g/L, CoCL2 0.025-0.03g/L; Cupric sulfate pentahydrate 0.02-0.03g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 100-200mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 100-200mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Beneficial effect of the present invention is following:
1, owing to N, P, K element proportioning in this prescription relatively meet the needs that the potato raw long hair is educated, so the output of spray culture is improved largely.
2, owing to added agricultural streptomycin, can reduce the mashed seedling of plant, seedling death phenomenon, effectively improve the surviving rate of plant at yam field planting initial stage and seedling phase.Knot adds metalaxyl the potato phase in nutritive medium, can effectively prevent disease such as late blight through the nutritive medium circulation propagate maybe, thereby improve the output of spray culture and the quality of micro potato.
3, because this prescription adds a certain proportion of urea in the potato seedling phase, improved the ratio of nitrogenous fertilizer in the nutritive medium, can promote the cauline leaf ramp, thereby the knot potato joint position in the groove is increased fast, for potato spray culture has been laid the high yield basis.
4, phosphate fertilizer and potash fertilizer can promote yam knot potato and potato piece to expand, this prescription adds a certain proportion of potassium primary phosphate tying the potato phase, have replenished the deficiency of potash fertilizer and phosphate fertilizer in the nutritive medium, can improve the output of potato spray culture greatly.
Through evidence; In potato spray culture, use nutrient solution prescription individual plant knot potato number of the present invention to reach 47.3; Expand into the potato number and reached 87.3; Improved 19.7% and 26.0% than MS culture medium prescription respectively, improved 13.7% and 10.2%, wherein expanded into the potato number and expand into several 76 of potato than domestic relevant report individual plant and improved 14.9% than the disclosed nutrient solution prescription of Chinese patent ZL98115898.6.
This shows; The present invention is through the proportioning of adjustment N, P, K element; Add sterilant such as agricultural streptomycin, metalaxyl and carry out the nutritive medium dynamic management; Improve the knot potato quantity and the potato piece size of potato spray culture greatly, and made full use of each effective constituent in the nutritive medium, reduced to produce and dropped into.
Embodiment
To combine embodiment to further describe below the present invention:
Embodiment 1
A, preparation mother liquor A: volume ratio is got saltpetre 39g/L by weight, potassium primary phosphate 24g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 37g/L, three water Sytron 4.9g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 47g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.83g/L by weight, boric acid 6.2g/L, four water manganous sulfate 22.3g/L; Zinc Sulphate Heptahydrate 8.6g/L, Sodium Molybdate Dihydrate 0.25g/L, CoCL2 0.025g/L; Cupric sulfate pentahydrate 0.025g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 100g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 100g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 100mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 100mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Embodiment 2
A, preparation mother liquor A: volume ratio is got saltpetre 32g/L by weight, potassium primary phosphate 27g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 39g/L, three water Sytron 4.2g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 52g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.89g/L by weight, boric acid 6.7g/L, four water manganous sulfate 22.7g/L; Zinc Sulphate Heptahydrate 8.3g/L, Sodium Molybdate Dihydrate 0.27g/L, CoCL2 0.028g/L; Cupric sulfate pentahydrate 0.022g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 150g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 150g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 200mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 200mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
Embodiment 3
A, preparation mother liquor A: volume ratio is got saltpetre 36g/L by weight, potassium primary phosphate 22g/L, and MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35g/L, three water Sytron 4.6g/L pour in the container, and the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 54g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.86g/L by weight, boric acid 6.5g/L, four water manganous sulfate 22.5g/L; Zinc Sulphate Heptahydrate 8.9g/L, Sodium Molybdate Dihydrate 0.22g/L, CoCL2 0.03g/L; Cupric sulfate pentahydrate 0.029g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 180g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 180g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 150mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium.
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 150mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.

Claims (2)

1. potato spray culture nutrient solution; It is characterized in that: said nutritive medium comprises mother liquor A, mother liquor B, mother solution C, mother liquor D and mother liquor E; Wherein said mother liquor A comprises the raw material of following weight volume ratio: saltpetre 30-40g/L, potassium primary phosphate 20-30g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L; Three water Sytron 4.0-5.0g/L, surplus is a water; Said mother liquor B comprises the raw material of following weight volume ratio: four water-calcium nitrate 45-55g/L, and surplus is a water; Said mother solution C comprises the raw material of following weight volume ratio: potassiumiodide 0.8-0.9g/L; Boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L, Zinc Sulphate Heptahydrate 8.0-9.0g/L; Sodium Molybdate Dihydrate 0.2-0.3g/L; CoCL2 0.025-0.03g/L, cupric sulfate pentahydrate 0.02-0.03g/L, surplus is a water; Said mother liquor D comprises the raw material of following weight volume ratio: urea 100-200g/L, and surplus is a water; Said mother liquor E comprises the raw material of following weight volume ratio: potassium primary phosphate 100-200g/L, surplus is a water.
2. preparation method who is used to prepare the said potato spray culture nutrient solution of claim 1, it is characterized in that: said method comprises the steps:
A, preparation mother liquor A: volume ratio is got saltpetre 30-40g/L by weight, potassium primary phosphate 20-30g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 35-40g/L; Three water Sytron 4.0-5.0g/L; Pour in the container, the tap water constant volume is used in the back that stirs, and gets final product to such an extent that mother liquor A is subsequent use;
B, preparation mother liquor B: volume ratio is got four water-calcium nitrate 45-55g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor B is subsequent use after stirring;
C, preparation mother solution C: volume ratio is got potassiumiodide 0.8-0.9g/L by weight, boric acid 6.0-7.0g/L, four water manganous sulfate 22-23g/L; Zinc Sulphate Heptahydrate 8.0-9.0g/L, Sodium Molybdate Dihydrate 0.2-0.3g/L, CoCL2 0.025-0.03g/L; Cupric sulfate pentahydrate 0.02-0.03g/L; Respectively with pouring volumetric flask into after the dissolving of a small amount of tap water, and add the tap water constant volume, pour into after fully shaking up and get final product to such an extent that mother solution C is subsequent use in the container;
D, preparation mother liquor D: volume ratio is got urea 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor D is subsequent use after stirring;
E, preparation mother liquor E: volume ratio is got potassium primary phosphate 100-200g/L and is poured in the container by weight, adds the tap water constant volume, gets final product to such an extent that mother liquor E is subsequent use after stirring;
Be 15-20 days the storage period of f, each mother liquor;
G, utilize mother liquor to prepare setting date and seedling phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor D dilution; 1000 times of mother solution C dilutions; Adding effective constituent simultaneously is the 100-200mg/L agricultural streptomycin, stirs to be made into setting date and seedling phase nutritive medium;
H, utilize mother liquor preparation knot potato phase nutritive medium: respectively with 100 times of mother liquor A, mother liquor B, mother liquor E dilution, 1000 times of mother solution C dilutions, adding effective constituent simultaneously is the 100-200mg/L metalaxyl, stirs to be made into knot potato phase nutritive medium.
CN201010106179A 2010-02-05 2010-02-05 Potato spray culture nutrient solution and preparation method thereof Expired - Fee Related CN101781148B (en)

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CN102010247B (en) * 2010-10-12 2013-04-24 丽江伯符农业科技发展有限公司 Nutrient solution for potato detoxified seedling reproduction
CN102417417B (en) * 2011-08-31 2014-01-15 南京市蔬菜科学研究所 Industrialized seedling raising nutrient solution as well as preparation method and application thereof
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CN110950718A (en) * 2020-01-16 2020-04-03 宁夏西吉县恒丰农业综合开发有限公司 Nutrient solution for producing original seeds of detoxified potatoes by aeroponic culture and preparation method thereof

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CN1961649A (en) * 2005-11-09 2007-05-16 石河子大学 A cultivation medium for test-tube seedling of detoxified potato and method for preparing the same
CN1843127A (en) * 2006-05-16 2006-10-11 沈阳化工学院 Plant source bacteriocide for agriculture

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