CN101735947A - Soil anammox bacterium gathering system simulating natural environment - Google Patents

Abstract

The invention discloses a soil anammox bacteria enrichment system simulating natural environment. It includes water inlet tank, peristaltic pump, time controller, reactor main body, nozzle, soil column, disc with holes, support, drainage pipe, sedimentation area, mud discharge pipe, packing layer, mud inlet, disc hole , Nozzle round hole, the lower part of the reactor main body is provided with brackets, the reactor main body is provided with nozzles, soil columns, discs with holes, packing layer, and sedimentation area in sequence from top to bottom, and the bottom of the reactor main body is provided with drainage pipes and sludge discharge A plurality of mud inlets are provided on the side wall of the soil column of the main body of the reactor, and the upper end of the nozzle is connected with the peristaltic pump and the water inlet tank in sequence, and the peristaltic pump is connected with the time controller. Advantages of the present invention: 1) Take the corresponding depth of soil layer in the soil column to establish a soil column simulating the natural environment, which is conducive to the enrichment of anammox bacteria in the soil; 2) The time control component realizes the intermittent water inflow of the reactor ; 3) This enrichment system completely simulates the original ecology, and can enrich the anammox bacteria in the soil ecosystem.

Description

Soil Anammox Bacteria Enrichment System Simulating Natural Environment

technical field

The invention relates to a soil anammox bacteria enrichment system simulating natural environment.

Background technique

Anaerobic ammonia oxidation (Anammox) is a process in which ammonia nitrogen reacts with nitrite nitrogen to generate nitrogen gas under anaerobic conditions. From the perspective of electron donor, anammox is an oxidation reaction of ammonia, similar to nitrification; but from the perspective of electron acceptor, anammox is a reduction reaction of nitrite, similar to reverse reaction. nitrification. That is to say, anaerobic ammonia oxidation can be regarded as both nitrification and denitrification. In the nitrogen cycle, both nitrification and denitrification are involved, which changes the traditional nitrification-denitrification nitrogen cycle mode. The discovery of anaerobic ammonium oxidation has added new content to the microbial nitrogen cycle. Anaerobic ammonium oxidation was first discovered in artificial wastewater treatment systems, but anaerobic ammonium oxidation reactions are also commonly found in natural ecosystems, such as in marine ecosystems, lake sediments, estuary sediments, soil groundwater treatment systems, etc. Anammox activity was detected in the system and was confirmed to play an important role. The contribution of ANAMMOX to the global nitrogen cycle cannot be ignored.

The soil nitrogen cycle is an important part of the global nitrogen cycle. Its nitrogen transformation process has a huge impact on the global nitrogen cycle. There are anoxic and anaerobic layers, aerobic and anoxic interfaces in the soil ecosystem. From the perspective of the conditions for anaerobic ammonium oxidation, it is a suitable environment for anaerobic ammonium oxidation, but it is an anaerobic environment for soil nitrogen cycle. Oxyammoxidation reports are few. In marine ecosystems, the contribution of anammox to the nitrogen cycle can reach 30-50%, but in soil ecosystems, the traditional soil nitrification-denitrification nitrogen cycle mode caused by the intervention of anammox reaction Neither the changes nor the contribution of each link in the soil nitrogen cycle have been reported. Therefore, to find out the soil anammox process is of great significance for improving the soil nitrogen cycle model and even the global nitrogen cycle model.

The present invention establishes a soil anammox bacteria enrichment system by simulating a natural soil ecosystem, enriches soil anammox bacteria, and expands the population range of anammox bacteria. The contribution of soil nitrogen cycle and the further development of anammox process lay a good foundation.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies of the prior art and provide a soil anammox bacteria enrichment system simulating the natural environment.

The soil anammox bacteria enrichment system simulating the natural environment includes water inlet tank, peristaltic pump, time controller, reactor body, nozzle, soil column, disc with holes, bracket, drain pipe, sedimentation area, and sludge discharge pipe , packing layer, mud intake, disc hole, nozzle hole, the lower part of the reactor main body is provided with brackets, and the reactor main body is provided with nozzles, soil columns, discs with holes, packing layer, sedimentation The bottom of the main body of the reactor is provided with drainage pipes and mud discharge pipes, and multiple mud inlets are provided on the side wall of the soil column of the main body of the reactor. The upper end of the nozzle is connected with the peristaltic pump and the water inlet tank in sequence. device connection.

The filler layer is pebbles of different sizes and shapes, with a particle diameter of 10-40 mm. The volume ratio of the soil column to the filler layer is 5.5-6:1. The precipitation area is conical, and the volume ratio of the precipitation area to the reactor main body is 5-6%. The diameter of the disc is the same as the inner diameter of the reactor, and small holes with a diameter of 3-5 mm are uniformly distributed in the disc. The distance between the plurality of mud-taking ports is 8-9 cm. The time controller controls the timing power-on and power-off of the peristaltic pump through programming, so that the peristaltic pump is fed with water every 3-3.2 hours, and the water-intake time is 2-3 minutes each time. The diameter of the nozzle is the same as the inner diameter of the reactor, the nozzle is covered with nozzles, and the diameter of the nozzle is 1-2 mm. The aspect ratio of the reactor main body is 4-5:1. The soil column is the soil of paddy field.

Compared with the prior art, the present invention has beneficial effects: 1) The main body of the reactor is columnar, and the corresponding depth of soil layer is taken in the reactor to establish a soil column simulating the natural environment, maintain the original ecological system of the soil, and ensure the soil flora The original state of the structure is conducive to the enrichment of anammox bacteria in the soil; 2) The setting of the reactor packing layer can effectively prevent the blockage of the water flow in the reaction and the large loss of soil, and has a good biological retention capacity. And can play a supporting role; 3) The perforated partition with holes between the soil column layer and the filler layer can effectively block the soil, reduce soil loss, and make the water in the soil column layer flow down smoothly; 4) The time control equipment realizes The intermittent water inflow to the reactor can generate a large water pressure instantaneously, which can make the connected spray equipment produce a large number of small water droplets, and realize the uniform water distribution in the reactor; 5) This enrichment system completely simulates the original ecology, and can Enriching the anammox bacteria in the soil ecosystem and expanding the population range of anammox bacteria can lay a good foundation for in-depth research on soil anammox and its contribution to the soil nitrogen cycle.

Description of drawings

Fig. 1 is a schematic diagram of the structure of the soil anammox enrichment system simulating the natural environment;

Fig. 2 is a structural schematic diagram of the disc with holes of the present invention;

Fig. 3 is a schematic diagram of the nozzle structure of the present invention;

In the figure: water inlet tank 1, peristaltic pump 2, time controller 3, reactor main body 4, nozzle 5, soil column 6, disc with holes 7, bracket 8, drain pipe 9, sedimentation area 10, mud discharge pipe 11 , Filling layer 12, get mud opening 13, disc circular hole 14, nozzle circular hole 15.

Detailed ways

As shown in the accompanying drawing, the soil anammox bacteria enrichment system simulating the natural environment includes a water inlet tank 1, a peristaltic pump 2, a time controller 3, a reactor body 4, a nozzle 5, a soil column 6, and a disc with holes 7 , support 8, drainage pipe 9, settling area 10, mud discharge pipe 11, packing layer 12, mud intake 13, disc hole 14, nozzle hole 15, the lower part of the reactor main body 4 is provided with a support 8, and the reactor main body 4 is provided with nozzle 5, soil column 6, perforated disc 7, packing layer 12, sedimentation area 10 in order from top to bottom, and the bottom of reactor main body 4 is provided with drainage pipe 9 and mud discharge pipe 11. 4. A plurality of mud intakes 13 are established on the side wall of the soil column 6. The upper end of the nozzle 5 is connected with the peristaltic pump 2 and the water inlet tank 1 in sequence, and the peristaltic pump 2 is connected with the time controller 3.

The filler layer 12 is pebbles of different sizes and shapes, with a particle diameter of 10-40mm. The volume ratio of the soil column 6 to the filler layer 12 is 5.5˜6:1. The precipitation zone 10 is conical, and the volume ratio of the precipitation zone 10 to the reactor main body 4 is 5-6%. The diameter of the disc 7 is the same as the inner diameter of the reactor, and small holes 14 with a diameter of 3-5 mm are evenly distributed in the disc. The distance between the plurality of mud-taking ports 13 is 8-9 cm. The time controller 3 controls the timing power on and off of the peristaltic pump 2 through programming, so that the peristaltic pump feeds water every 3 to 3.2 hours, and the time for each water feed is 2 to 3 minutes. The diameter of the nozzle 5 is the same as the inner diameter of the reactor, and the nozzle 15 is covered with the nozzle, and the diameter of the nozzle is 1-2 mm. The aspect ratio of the reactor main body 4 is: the soil column 6 is the soil of a paddy field.

The main body of the reactor of the present invention is made of plexiglass and has a cylindrical shape. The main body of the reactor is sequentially provided with a nozzle, a soil column, a disc with holes, a packing layer, and a sedimentation area from top to bottom. A certain space is reserved on the upper part of the soil column for temporarily accumulating water. The soil column layer is filled with soil layers of different depths to maintain the original soil ecosystem and ensure the original state of the soil flora structure, which is conducive to the enrichment of anammox bacteria in the soil; the filler layer is filled with pebbles of different sizes and shapes, which can It effectively prevents the clogging of the water flow in the reaction and the massive loss of soil, plays the role of filtering and supporting, and ensures that the reactor has a good bioretention capacity. There is a settling area at the bottom of the main body of the reactor, which can settle part of the soil brought out from the upper part at the initial stage of operation, preventing the blockage of the outlet pipe. Excessive soil deposited in the settling area can be discharged from the sludge discharge pipe at the bottom of the reactor. The natural lake water is used as the influent, and an appropriate amount of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is artificially added to it, in order to accelerate the enrichment of soil anammox bacteria. The peristaltic pump is controlled by the command set by the time controller to realize intermittent water feeding in the reactor. After the incoming water passes through the spray device, a large number of small water droplets are formed, which are evenly distributed on the surface of the soil layer of the reactor. The water body slowly infiltrates in the soil column, and enters the filler layer after passing through the disc with round holes. Part of the soil that is brought out can be intercepted by the filler, and the water body continues to flow down and flows out through the outlet pipe. The entire enrichment system realizes intermittent operation.

Claims (10)

1. the soil anaerobic ammonoxidation bacterium concentrating system of a simulating natural environment, it is characterized in that comprising inlet chest (1), peristaltic pump (2), time controller (3), reactor body (4), shower nozzle (5), earth pillar (6), disk with holes (7), support (8), water shoot (9), settling region (10), shore pipe (11), packing layer (12), get mud mouth (13), disk circular hole (14), shower nozzle circular hole (15), reactor body (4) bottom is provided with support (8), be provided with shower nozzle (5) in the reactor body (4) from top to bottom successively, earth pillar (6), disk with holes (7), packing layer (12), settling region (10), reactor body (4) bottom is provided with water shoot (9), shore pipe (11), put at reactor body (4) on the sidewall of earth pillar (6) and establish a plurality of mud mouths (13) of getting, shower nozzle (5) upper end and peristaltic pump (2), inlet chest (1) connects successively, and peristaltic pump (2) is connected with time controller (3).

2. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described packing layer (12) is the pebbles of different size shape, and particle diameter is 10～40mm.

3. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that the described earth pillar (6) and the volume ratio of packing layer (12) are 5.5～6: 1.

4. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described settling region (10) is taper, the volume ratio 5～6% of settling region (10) and reactor body (4).

5. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that the diameter of described disk (7) is identical with reactor inside diameter, and the uniform distribution aperture is the aperture (14) of 3～5mm in the disk.

6. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described a plurality of spacing of getting mud mouth (13) is 8～9cm.

7. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1, it is characterized in that the timing power on/off of described time controller (3) by programming Control peristaltic pump (2), make peristaltic pump advance water one time, each flooding time 2～3min every 3～3.2h.

8. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that the diameter of described shower nozzle (5) is identical with reactor inside diameter, and shower nozzle is covered with nozzle (15), and nozzle diameter is 1～2mm.

9. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that the aspect ratio of described reactor body (4) is: 4～5: 1.

10. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that described earth pillar (6) is the soil of rice terrace.

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