CN101735947A - Soil anammox bacterium gathering system simulating natural environment - Google Patents
Soil anammox bacterium gathering system simulating natural environment Download PDFInfo
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- CN101735947A CN101735947A CN200910155264A CN200910155264A CN101735947A CN 101735947 A CN101735947 A CN 101735947A CN 200910155264 A CN200910155264 A CN 200910155264A CN 200910155264 A CN200910155264 A CN 200910155264A CN 101735947 A CN101735947 A CN 101735947A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/18—Fixed or packed bed
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/22—Settling tanks; Sedimentation by gravity
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Abstract
The invention discloses a soil anammox bacterium gathering system simulating a natural environment, comprising a water intake tank, a peristaltic pump, a time controller, a reactor main body, a spray head, an earth pillar, a disc with holes, a support, a water discharging pipe, a precipitation zone, a sludge discharging pipe, a packing layer, a plurality of sludge taking ports, circular holes of the disc and circular holes of the spray head, wherein the lower part of the reactor main body is provided with the support, the reactor main body is sequentially provided with the spray head, the earth pillar, the disc with holes, the packing layer and the precipitation zone from top to bottom, the bottom of the reactor main body is provided with the water discharging pipe and the sludge discharging pipe, the sludge taking ports are arranged on the side wall for placing the earth pillar on the reactor main body, the upper end of the spray head is sequentially connected with the peristaltic pump and the water intake tank, and the peristaltic pump is connected with the time controller. The soil anammox bacterium gathering system has the advantages that an earth layer with the corresponding depth is put in the earth pillar to establish the earth pillar simulating the natural environment so as to be beneficial to the gathering of anammox bacteria in soil; the time controlling assembly realizes the intermittent water intake of the reactor; and the gathering system completely simulates the original ecology and can gather the anammox bacteria in a soil ecosystem.
Description
Technical field
The present invention relates to a kind of soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment.
Background technology
(Anaerobic ammonia oxidation Anammox) is meant under anaerobic ammonia nitrogen and the reaction of nitrous acid nitrogen to Anammox, generates the process of nitrogen.From the angle of electron donor, Anammox is the oxidizing reaction of an ammonia, is similar to nitrification; But from the angle of electron acceptor(EA), Anammox then is the reduction reaction of a nitrite, is similar to denitrification.That is to say that Anammox both can be a nitrification, can be denitrification also, has got involved simultaneously in nitrification and the denitrification in Nitrogen Cycling, has changed traditional nitrated-denitrification nitrogen cycle pattern.Circulation has increased new content to microorganism nitrogen in the discovery of Anammox effect.Anammox is found in artificial wastewater's treatment system the earliest, but the Anammox reaction also is prevalent in the natural ecosystems, as in Marine ecosystems, all detected anaerobic ammoxidation activity in the ecosystems such as lake sediment, estuarine deposit, soil groundwater treatment system, and be proved and have vital role.The contribution that Anammox is risen in global Nitrogen Cycling can not be ignored.
The Soil Nitrogen circulation is an important component part of global nitrogen cycle.Its nitrogen transformation process has tremendous influence to global nitrogen cycle.Having anoxic and anaerobic layer, aerobic and anoxybiotic interface in soil ecosystem, from the condition of Anammox takes place, is the suitable environment that Anammox takes place, but for the report of Anammox in the soil nitrogen circulation seldom.In Marine ecosystems, Anammox can reach 30-50% to the contribution of nitrogen cycle, but in soil ecosystem, the contribution that the change of traditional soil nitrification-denitrification nitrogen cycle pattern that the intervention of reacting owing to Anammox causes and each link are risen in the Soil Nitrogen circulation does not all appear in the newspapers.Therefore verify soil anaerobic ammonia oxidation process, for improving the soil nitrogen circulation pattern and all having great importance to global Nitrogen Cycling pattern.
The present invention is by the simulating nature soil ecosystem, set up soil anaerobic ammonoxidation bacterium concentrating system, enrichment soil anaerobic ammonia oxidation bacteria, enlarge the population scope of anaerobic ammonia oxidizing bacteria, lay a good foundation with further exploitation anaerobic ammonia oxidation process for furtheing investigate anaerobic ammonia oxidizing bacteria and the contribution in the soil nitrogen circulation thereof.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment is provided.
The soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment comprises inlet chest, peristaltic pump, time controller, reactor body, shower nozzle, earth pillar, disk with holes, support, water shoot, the settling region, shore pipe, packing layer, get the mud mouth, the disk circular hole, the shower nozzle circular hole, the reactor body bottom is provided with support, be provided with shower nozzle in the reactor body from top to bottom successively, earth pillar, disk with holes, packing layer, the settling region, the reactor body bottom is provided with water shoot, shore pipe, put at reactor body on the sidewall of earth pillar and establish a plurality of mud mouths of getting, shower nozzle upper end and peristaltic pump, inlet chest connects successively, and peristaltic pump is connected with time controller.
Described packing layer is the pebbles of different size shape, and particle diameter is 10～40mm.The volume ratio of described earth pillar and packing layer is 5.5～6: 1.Described settling region is taper, the volume ratio 5～6% of settling region and reactor body.The diameter of described disk is identical with reactor inside diameter, and the uniform distribution aperture is the aperture of 3～5mm in the disk.Described a plurality of spacing of getting the mud mouth is 8～9cm.Described time controller makes peristaltic pump advance water one time every 3～3.2h by the timing power on/off of programming Control peristaltic pump, each flooding time 2～3min.The diameter of described shower nozzle is identical with reactor inside diameter, and shower nozzle is covered with nozzle, and nozzle diameter is 1～2mm.The aspect ratio of described reactor body is: 4～5: 1.Described earth pillar is the soil of rice terrace.
The beneficial effect that the present invention compared with prior art has: 1) reactor body is column, get the respective depth soil layer in reactor, set up the earth pillar of simulating natural environment, keep soil ecosystem system, guarantee the original existence of soil bacteria group structure, helped the enrichment of anaerobic ammonia oxidizing bacteria in the soil; 2) setting of reactor filler layer, the obstruction of current and a large amount of losses of soil in can effectively preventing to react have preferably biology and hold the ability of staying, and can play a supporting role; 3) dividing plate with holes with holes can effectively intercept soil between earth pillar layer and packing layer, reduces the loss of soil, and makes the water of earth pillar layer dirty smoothly; 4) time control equipment has been realized the intermittence water inlet of reactor, and the hydraulic pressure that its instantaneous generation is bigger can make the sprinkling equipment of its connection produce a large amount of little water droplets, has realized water distribution uniformity in the reactor; 5) this enrichment system is simulated ecosystem fully, but the anaerobic ammonia oxidizing bacteria in the enrichment soil ecosystem, the population scope of expansion anaerobic ammonia oxidizing bacteria can be further investigation soil anaerobic ammoxidation and the contribution in the soil nitrogen circulation lays a good foundation.
Description of drawings
Fig. 1 is the soil anaerobic ammonia oxidation enrichment system structural representation of simulation natural surroundings;
Fig. 2 is a disc structure synoptic diagram with holes of the present invention;
Fig. 3 is a nozzle structure synoptic diagram of the present invention;
Among the figure: inlet chest 1, peristaltic pump 2, time controller 3, reactor body 4, shower nozzle 5, earth pillar 6, disk with holes 7, support 8, water shoot 9, settling region 10, shore pipe 11, packing layer 12, get mud mouth 13, disk circular hole 14, shower nozzle circular hole 15.
Embodiment
The soil anaerobic ammonoxidation bacterium concentrating system of simulating natural environment comprises inlet chest 1 as shown in drawings, peristaltic pump 2, time controller 3, reactor body 4, shower nozzle 5, earth pillar 6, disk 7 with holes, support 8, water shoot 9, settling region 10, shore pipe 11, packing layer 12, get mud mouth 13, disk circular hole 14, shower nozzle circular hole 15, reactor body 4 bottoms are provided with support 8, be provided with shower nozzle 5 in the reactor body 4 from top to bottom successively, earth pillar 6, disk 7 with holes, packing layer 12, settling region 10, reactor body 4 bottoms are provided with water shoot 9, shore pipe 11, put at reactor body 4 on the sidewall of earth pillar 6 and establish a plurality of mud mouths 13 of getting, shower nozzle 5 upper ends and peristaltic pump 2, inlet chest 1 connects successively, and peristaltic pump 2 is connected with time controller 3.
Described packing layer 12 is the pebbles of different size shape, and particle diameter is 10～40mm.Described earth pillar 6 is 5.5～6: 1 with the volume ratio of packing layer 12.Described settling region 10 is taper, the volume ratio 5～6% of settling region 10 and reactor body 4.The diameter of described disk 7 is identical with reactor inside diameter, and the uniform distribution aperture is the aperture 14 of 3～5mm in the disk.Described a plurality of spacing of getting mud mouth 13 is 8～9cm.Described time controller 3 makes peristaltic pump advance water one time every 3～3.2h by the timing power on/off of programming Control peristaltic pump 2, each flooding time 2～3min.The diameter of described shower nozzle 5 is identical with reactor inside diameter, and shower nozzle is covered with nozzle 15, and nozzle diameter is 1～2mm.The aspect ratio of described reactor body 4 is: described earth pillar 6 is the soil of rice terrace.
Reactor body of the present invention is made by synthetic glass, and it is cylindric that profile is.Be provided with shower nozzle, earth pillar, disk with holes, packing layer, settling region in the reactor body from top to bottom successively.Reserve certain space on earth pillar top, be used for temporarily accumulating into water.The earth pillar layer has been filled the soil layer of different depths, keeps soil ecosystem system, guarantees the original existence of soil bacteria group structure, helps the enrichment of anaerobic ammonia oxidizing bacteria in the soil; Packing layer is filled the pebbles of different size shape, and the obstruction of current and a large amount of losses of soil in can effectively preventing to react are played and filtered and supporting role, has guaranteed that reactor has preferably biology and holds the ability of staying.The reactor body bottom is provided with the settling region, can precipitate the part soil of taking out of on initial operating stage top, has prevented the obstruction of rising pipe, and the sedimentary too much soil in settling region can be discharged from the shore pipe of reactor bottom.And with natural lake water as water inlet, artificially add an amount of ammonia nitrogen, nitrite nitrogen and nitre nitrogen to it, purpose is to accelerate the enrichment of soil anaerobic ammonia oxidation bacteria.Control intermittent water inflow in the peristaltic pump realization response device by the order that time controller is provided with.Water inlet forms a large amount of little water droplets behind spray equipment, evenly spill to be distributed in reactor soil layer surface.Water body slowly oozes down in earth pillar, enters packing layer behind the disk of band circular hole, and the soil that part is taken out of can be tackled by filler, and water body then continues dirty, flows out through rising pipe.Whole enrichment system has been realized intermittent operation.
Claims (10)
1. the soil anaerobic ammonoxidation bacterium concentrating system of a simulating natural environment, it is characterized in that comprising inlet chest (1), peristaltic pump (2), time controller (3), reactor body (4), shower nozzle (5), earth pillar (6), disk with holes (7), support (8), water shoot (9), settling region (10), shore pipe (11), packing layer (12), get mud mouth (13), disk circular hole (14), shower nozzle circular hole (15), reactor body (4) bottom is provided with support (8), be provided with shower nozzle (5) in the reactor body (4) from top to bottom successively, earth pillar (6), disk with holes (7), packing layer (12), settling region (10), reactor body (4) bottom is provided with water shoot (9), shore pipe (11), put at reactor body (4) on the sidewall of earth pillar (6) and establish a plurality of mud mouths (13) of getting, shower nozzle (5) upper end and peristaltic pump (2), inlet chest (1) connects successively, and peristaltic pump (2) is connected with time controller (3).
2. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described packing layer (12) is the pebbles of different size shape, and particle diameter is 10～40mm.
3. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that the described earth pillar (6) and the volume ratio of packing layer (12) are 5.5～6: 1.
4. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described settling region (10) is taper, the volume ratio 5～6% of settling region (10) and reactor body (4).
5. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that the diameter of described disk (7) is identical with reactor inside diameter, and the uniform distribution aperture is the aperture (14) of 3～5mm in the disk.
6. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1 is characterized in that described a plurality of spacing of getting mud mouth (13) is 8～9cm.
7. the soil anaerobic ammonoxidation bacterium concentrating system of a kind of simulating natural environment according to claim 1, it is characterized in that the timing power on/off of described time controller (3) by programming Control peristaltic pump (2), make peristaltic pump advance water one time, each flooding time 2～3min every 3～3.2h.
8. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that the diameter of described shower nozzle (5) is identical with reactor inside diameter, and shower nozzle is covered with nozzle (15), and nozzle diameter is 1～2mm.
9. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that the aspect ratio of described reactor body (4) is: 4～5: 1.
10. the soil anaerobic ammonia oxidation enrichment system of a kind of simulating natural environment according to claim 1 is characterized in that described earth pillar (6) is the soil of rice terrace.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200910155264 CN101735947B (en) | 2009-12-10 | 2009-12-10 | Soil anammox bacterium gathering system simulating natural environment |
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| CN 200910155264 CN101735947B (en) | 2009-12-10 | 2009-12-10 | Soil anammox bacterium gathering system simulating natural environment |
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| CN101735947B CN101735947B (en) | 2013-02-06 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955826A (en) * | 2010-08-27 | 2011-01-26 | 浙江大学 | Integrated aerobic/anaerobic safety methane biological desulfurization device |
| CN102267762A (en) * | 2011-07-12 | 2011-12-07 | 天津城市建设学院 | Intermittent water seepage type salt-alkali resistant plant-soil ecological filter bed |
| CN102464406A (en) * | 2010-11-09 | 2012-05-23 | 中国科学院生态环境研究中心 | Method for realizing denitrification of anaerobic ammonium oxidation by utilizing constructed wetland |
| CN102531177A (en) * | 2010-12-13 | 2012-07-04 | 中国科学院生态环境研究中心 | Method for improving nitration reaction rate of wetland system |
| CN107064463A (en) * | 2017-04-28 | 2017-08-18 | 中国农业科学院农田灌溉研究所 | Buried soil column leaching device |
| CN108802336A (en) * | 2018-06-26 | 2018-11-13 | 青岛海洋地质研究所 | It is a kind of to be used to measure wetland purification polluted by nitrogen efficiency and the stable state culture device of nitrogen cycle |
| CN111547866A (en) * | 2020-06-11 | 2020-08-18 | 北京工业大学 | Device and method for culturing and enriching anaerobic ammonium oxidation sludge |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955826A (en) * | 2010-08-27 | 2011-01-26 | 浙江大学 | Integrated aerobic/anaerobic safety methane biological desulfurization device |
| CN101955826B (en) * | 2010-08-27 | 2013-01-30 | 浙江大学 | Integrated aerobic/anaerobic safety methane biological desulfurization device |
| CN102464406A (en) * | 2010-11-09 | 2012-05-23 | 中国科学院生态环境研究中心 | Method for realizing denitrification of anaerobic ammonium oxidation by utilizing constructed wetland |
| CN102531177A (en) * | 2010-12-13 | 2012-07-04 | 中国科学院生态环境研究中心 | Method for improving nitration reaction rate of wetland system |
| CN102267762A (en) * | 2011-07-12 | 2011-12-07 | 天津城市建设学院 | Intermittent water seepage type salt-alkali resistant plant-soil ecological filter bed |
| CN107064463A (en) * | 2017-04-28 | 2017-08-18 | 中国农业科学院农田灌溉研究所 | Buried soil column leaching device |
| CN108802336A (en) * | 2018-06-26 | 2018-11-13 | 青岛海洋地质研究所 | It is a kind of to be used to measure wetland purification polluted by nitrogen efficiency and the stable state culture device of nitrogen cycle |
| CN108802336B (en) * | 2018-06-26 | 2023-10-31 | 青岛海洋地质研究所 | A steady state culture equipment for determining wetland purifies nitrogen pollution efficiency and nitrogen circulation |
| CN111547866A (en) * | 2020-06-11 | 2020-08-18 | 北京工业大学 | Device and method for culturing and enriching anaerobic ammonium oxidation sludge |
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| CN101735947B (en) | 2013-02-06 |
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