CN101195803A - Anaerobic ammonoxidation bacterium concentrating device - Google Patents
Anaerobic ammonoxidation bacterium concentrating device Download PDFInfo
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- CN101195803A CN101195803A CNA2007101648399A CN200710164839A CN101195803A CN 101195803 A CN101195803 A CN 101195803A CN A2007101648399 A CNA2007101648399 A CN A2007101648399A CN 200710164839 A CN200710164839 A CN 200710164839A CN 101195803 A CN101195803 A CN 101195803A
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Abstract
The invention discloses a high- efficient enrichment device of the anaerobic ammonium oxidation bacteria. The lower circular cylinder of the main body of the device is connected with an upper circular cylinder through an increasing coupling, a sealing cover is arranged at the top part of the upper circular cylinder, an air outlet pipe is arranged on the sealing cover and is connected with a gas washing bottle, grain bamboo charcoal carriers are installed at the lower part of the lower circular cylinder, a primary gas-liquid-solid three phase separating component is arranged inside the upper circular cylinder, a secondary mud water separating component is arranged on the outer side, a grain sludge blocking component is arranged between the secondary mud water separating component and the upper circular cylinder, a grain sludge collecting opening is arranged at the lower part of the secondary mud water separating component, a overflow dam is arranged at the upper part of the grain sludge blocking component and is connected with a water outlet tank through a water collecting pipe, a water inlet pipe is arranged at the bottom part of the lower circular cylinder and is connected with the water inlet tank through a water inlet pump, a returning pipeline is arranged on the side wall at the middle part of the upper circular cylinder, and the returning pipeline passing a returning pump directly enters into the water inlet pipe through a three way pipe. The invention can block the anaerobic ammonium oxidation bacteria on the surface of the carrier, and obtains the enrichment culture of the anaerobic ammonium oxidation bacteria with high activity; the culture presents red, and the character is obvious; the culture is grain sludge, and is easy to be obtained.
Description
Technical field
The present invention relates to environmental protecting device, relate in particular to a kind of anaerobic ammonoxidation bacterium concentrating device.
Background technology
Anaerobic ammonia oxidizing bacteria is the newfound anaerobism autotroph of a class, ammonia nitrogen and nitrite nitrogen can be changed into nitrogen in 1: 1.32 ratio, have special cellularstructure and physiological characteristic, scientific research value and exploitation are worth all good, and extremely organic sphere and environmental engineering circle are paid close attention to.But owing to reach 11d the generation time of anaerobic ammonia oxidizing bacteria, and cell yield is very low, be difficult to obtain the enrichment culture thing with traditional micro-biological process, therefore study new enriching method and device, have important practical significance for the research that promotes Anammox mechanism and the application of anaerobic ammonia oxidation process.
Summary of the invention
The purpose of this invention is to provide a kind of anaerobic ammonoxidation bacterium concentrating device.
Anaerobic ammonoxidation bacterium concentrating device has device body, the following cylinder of device body is connected with last cylinder through increaser, last cylinder top is provided with sealing cover, sealing cover is provided with vapor pipe and is connected with scrubbing bottle, particle bamboo charcoal carrier is equipped with in following cylinder bottom, last cylinder interior is established the gas-liquid-solid three phase separation assembly of one-level, secondary mud-water separation assembly is established in the gas-liquid-solid three phase separation assembly of the one-level outside, between secondary mud-water separation assembly and last cylinder, be provided with the granule sludge intercepting component, granule sludge intercepting component below, last cylinder side wall is provided with granule sludge and collects mouth, granule sludge intercepting component top is provided with overflow weir, overflow weir is connected with water tank through header, following cylinder bottom is provided with water inlet pipe, water inlet pipe is connected with inlet chest through intake pump, last cylinder middle part sidewall is provided with return line, and return line directly enters water inlet pipe through reflux pump by threeway.
The described diameter of cylinder down is 12～14cm, and the diameter of last cylinder is 18～20cm.Particle bamboo charcoal carrier, its particle diameter are 2～3mm, and shared volume is 2/3～3/4 of following cylinder volume.The gas-liquid-solid three phase separation assembly of one-level is funnel-form, flare opening diameter 15～17cm, and funnel neck length 11.5～12.5cm, diameter are 4～6cm, apart from device top cover 4～5cm.Secondary mud-water separation assembly is cylinder, is fixed on the device top cover, long 11～12cm, and diameter 8～10cm in height overlaps 6～8cm with the gas-liquid-solid three-phase separating device of one-level, and both spacings are 2～3cm.The granule sludge intercepting component is the plastics reticulated structure, and aperture 2～3mm is positioned at 5～6cm place under the liquid level.Granule sludge is collected mouth, is positioned at 1～2cm place, granule sludge intercepting component below.Device body selects for use transparent synthetic glass to make.
Anaerobic ammonia oxidizing bacteria can form the outer polymer of a large amount of born of the same parents, has adhesion property, and filling carrier in following cylinder can make its apposition growth in carrier surface, plays the effect of staying thalline of holding.Because the granule sludge that the anaerobic ammonia oxidizing bacteria apposition growth forms is the combination naturally of various microorganisms, have the compound group of optimization, active higher, function is stronger.Two two cylinders that diameter is different are set, can reduce current and gas velocity in the cylinder, be convenient to mud and separate, make part mud return down cylinder again by gravity settling, the gas of generation then can enter air chamber and discharge by filler neck.Between secondary mud-water separation assembly and last cylinder, establish the netted granule sludge intercepting component of plastics, be convenient to catch granule sludge.Select for use transparent organic glass as device materials, be convenient to observe the development trend of red granules mud, and collect mouth from mud and obtain granule sludge.
Description of drawings
Accompanying drawing is an anaerobism ammonia oxygen bacterium concentrating device structural representation;
Among the figure: inlet chest 1, intake pump 2, water inlet pipe 3, particle bamboo charcoal carrier 4, increaser 5, the gas-liquid-solid three phase separation assembly 6 of one-level, return line 8, reflux pump 9, overflow weir 10, header 11, water tank 12, vapor pipe 13, gas cylinder 14, granule sludge intercepting component 15, granule sludge reclaim mouth 16, sealing cover 17, last cylinder 18, following cylinder 19.
Embodiment
As shown in drawings, anaerobic ammonoxidation bacterium concentrating device has device body, the following cylinder 18 of device body is connected with last cylinder 19 through increaser 5, last cylinder 19 tops are provided with sealing cover 17, sealing cover 17 is provided with vapor pipe 13 and is connected with scrubbing bottle 14, particle bamboo charcoal carrier 4 is equipped with in following cylinder 18 bottoms, the gas-liquid-solid three phase separation assembly 6 of one-level is established in last cylinder 19 inside, secondary mud-water separation assembly 7 is established in the gas-liquid-solid three phase separation assembly of one-level 6 outsides, between secondary mud-water separation assembly 7 and last cylinder 19, be provided with granule sludge intercepting component 15, granule sludge intercepting component 15 belows, last cylinder 19 sidewalls are provided with granule sludge and collect mouth 16, granule sludge intercepting component 15 tops are provided with overflow weir 10, overflow weir 10 is connected with water tank 12 through header 11, following cylinder 18 bottoms are provided with water inlet pipe 3, water inlet pipe 3 is connected with inlet chest 1 through intake pump 2, and last cylinder 19 middle part sidewalls are provided with return line 8, and return line 8 directly enters water inlet pipe 3 through reflux pump 9 by threeway.
The described diameter of cylinder 18 down is 12～14cm, and the diameter of last cylinder 19 is 18～20cm.Particle bamboo charcoal carrier 4, its particle diameter are 2～3mm, and shared volume is 2/3～3/4 of following cylinder volume.The gas-liquid-solid three phase separation assembly 6 of one-level is funnel-form, flare opening diameter 15～17cm, and funnel neck length 11.5～12.5cm, diameter are 4～6cm, apart from device top cover 4～5cm.Secondary mud-water separation assembly 7 is cylinder, is fixed on the device top cover 17, long 11～12cm, and diameter 8～10cm in height overlaps 6～8cm with the gas-liquid-solid three-phase separating device 6 of one-level, and both spacings are 2～3cm.Granule sludge intercepting component 15 is the plastics reticulated structure, and aperture 2～3mm is positioned at 5～6cm place under the liquid level.Granule sludge is collected mouth 16, is positioned at 1～2cm place, granule sludge intercepting component 15 belows.Device body selects for use transparent synthetic glass to make, and is convenient to the color and the dynamic change of anaerobic ammonia oxidizing bacteria in the observing device.
Anaerobic ammonoxidation bacterium concentrating device has device body, mainly by the different following cylinder 18 of two diameters and, go up cylinder 19 and constitute.Descending cylinder 18 inside that particle bamboo charcoal carrier 4 is housed, its particle diameter is 2-3mm, and shared volume is 2/3 of following cylinder volume.Be provided with water inlet pipe 3 in the device body bottom, water inlet pipe links to each other with intake pump 2, and the nutrient solution in the inlet chest 1 is pumped into device.Establish the gas-liquid-solid three phase separation assembly 6 of one-level in last cylinder 19 inside, when mixed solution flows through 6, the mud that is carried by current and air-flow precipitates again and returns down cylinder, and gas is discharged through scrubbing bottle 14 by vapor pipe 13 after entering air chamber, and current then continue to flow through secondary mud-water separation assembly 7.At this, mud is blocked once more, and returns cylinder down under downward current and action of gravity.Primary water after the separation flows through overflow weir 10, flows into water tank 12 through header 11.Between secondary mud-water separation assembly and last cylinder, establish the netted granule sludge intercepting component 15 of plastics, below this assembly, establish granule sludge and reclaim mouth 16.Sealing cover 17 is established in the device body upper end.Device is established return line 8 and reflux pump 9, and phegma directly enters water inlet pipe 3 through threeway, and effluent recycling can be alleviated the restraining effect of matrix to anaerobic ammonia oxidizing bacteria.
Adopt this device, with the nitrifying sludge of certain Gourmet Powder Factory and denitrification mud as seed sludge, with simulated wastewater as matrix, at pH7.5-8.5, under the condition that temperature is about 30 ℃, successfully enrichment anaerobic ammonia oxidizing bacteria.By 144 days cultivation, the particle bamboo charcoal carrier surface of device adhered to a large amount of red thalline.Under the combined action of current and air-flow, the part granule sludge moves up, and is held back by granule sludge intercepting component 15, can collect the mouth from mud as required and obtain.After testing, in these red granules mud, anaerobic ammonia oxidizing bacteria occupies absolute predominance, and anaerobic ammoxidation activity reaches 0.56 mgTN (mg
-1Protein) h
-1
Claims (8)
1. anaerobic ammonoxidation bacterium concentrating device, it is characterized in that: it has device body, the following cylinder 18 of device body is connected with last cylinder 19 through increaser 5, last cylinder 19 tops are provided with sealing cover 17, sealing cover 17 is provided with vapor pipe 13 and is connected with scrubbing bottle 14, particle bamboo charcoal carrier 4 is equipped with in following cylinder 18 bottoms, the gas-liquid-solid three phase separation assembly 6 of one-level is established in last cylinder 19 inside, secondary mud-water separation assembly 7 is established in the gas-liquid-solid three phase separation assembly of one-level 6 outsides, between secondary mud-water separation assembly 7 and last cylinder 19, be provided with granule sludge intercepting component 15, granule sludge intercepting component 15 belows, last cylinder 19 sidewalls are provided with granule sludge and collect mouth 16, granule sludge intercepting component 15 tops are provided with overflow weir 10, overflow weir 10 is connected with water tank 12 through header 11, following cylinder 18 bottoms are provided with water inlet pipe 3, water inlet pipe 3 is connected with inlet chest 1 through intake pump 2, and last cylinder 19 middle part sidewalls are provided with return line 8, and return line 8 directly enters water inlet pipe 3 through reflux pump 9 by threeway.
2. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that the described diameter of cylinder 18 down is 12～14cm, and the diameter of last cylinder 19 is 18～20cm.
3. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that described particle bamboo charcoal carrier 4, and its particle diameter is 2～3mm, and shared volume is 2/3～3/4 of following cylinder volume.
4. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that the gas-liquid-solid three phase separation assembly 6 of described one-level, is funnel-form, flare opening diameter 15～17cm, funnel neck length 11.5～12.5cm, diameter are 4～6cm, apart from device top cover 4～5cm.
5. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1, it is characterized in that described secondary mud-water separation assembly 7, be cylinder, be fixed on the device top cover 17, long 11～12cm, diameter 8～10cm in height overlaps 6～8cm with the gas-liquid-solid three-phase separating device 6 of one-level, and both spacings are 2～3cm.
6. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that described granule sludge intercepting component 15 is the plastics reticulated structure, and aperture 2～3mm is positioned at 5～6cm place under the liquid level.
7. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that described granule sludge collection mouth 16, is positioned at 1～2cm place, granule sludge intercepting component 15 belows.
8. a kind of anaerobic ammonoxidation bacterium concentrating device according to claim 1 is characterized in that described device body selects for use transparent synthetic glass to make.
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| CN2007101648399A CN101195803B (en) | 2007-12-25 | 2007-12-25 | Anaerobic ammonoxidation bacterium concentrating device |
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| CN2007101648399A CN101195803B (en) | 2007-12-25 | 2007-12-25 | Anaerobic ammonoxidation bacterium concentrating device |
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| CN101195803B CN101195803B (en) | 2011-07-27 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735947A (en) * | 2009-12-10 | 2010-06-16 | 浙江大学 | Soil anammox bacterium gathering system simulating natural environment |
| CN101863554A (en) * | 2010-06-04 | 2010-10-20 | 浙江大学 | Superhigh-efficiency anammox reactor |
| CN102219298A (en) * | 2010-03-12 | 2011-10-19 | 三菱丽阳株式会社 | biological treating device and biological treating method |
| CN102533529A (en) * | 2012-01-18 | 2012-07-04 | 浙江大学 | Magnetic-stirring gas-rising internal circulation denitrification type methane anaerobic oxidation bacterium enrichment device and method |
| CN103043791A (en) * | 2013-01-19 | 2013-04-17 | 桂林理工大学 | Method for lowering occurrence amount of nitrous oxide in anaerobic ammonia oxidization reactor |
| CN103145239A (en) * | 2013-03-11 | 2013-06-12 | 丽水学院 | Quick enriching reaction equipment for anammox |
| CN104556410A (en) * | 2015-01-09 | 2015-04-29 | 浙江大学 | Marine ammonia-oxidizing archaea enrichment device capable of automatically controlling dissolved oxygen |
| CN109502746A (en) * | 2018-11-26 | 2019-03-22 | 中广核环保产业有限公司 | Sewage treatment process based on Anammox |
| CN115259556A (en) * | 2022-07-26 | 2022-11-01 | 上海净豚环保科技有限公司 | Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004071324A2 (en) * | 2003-02-11 | 2004-08-26 | Intralytix, Inc. | Method to reduce, eliminate, or prevent bacterial pathogen colonization in eggs and/or poultry using bacteriophages |
| CN100529049C (en) * | 2006-05-12 | 2009-08-19 | 中国科学院地球化学研究所 | Magnetic bacteria fast-collection device |
| CN201162007Y (en) * | 2007-12-25 | 2008-12-10 | 浙江大学 | Anaerobic ammonia oxidation bacterium concentrating device |
-
2007
- 2007-12-25 CN CN2007101648399A patent/CN101195803B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735947A (en) * | 2009-12-10 | 2010-06-16 | 浙江大学 | Soil anammox bacterium gathering system simulating natural environment |
| CN102219298A (en) * | 2010-03-12 | 2011-10-19 | 三菱丽阳株式会社 | biological treating device and biological treating method |
| CN101863554A (en) * | 2010-06-04 | 2010-10-20 | 浙江大学 | Superhigh-efficiency anammox reactor |
| CN101863554B (en) * | 2010-06-04 | 2012-03-28 | 浙江大学 | Superhigh-efficiency anammox reactor |
| CN102533529A (en) * | 2012-01-18 | 2012-07-04 | 浙江大学 | Magnetic-stirring gas-rising internal circulation denitrification type methane anaerobic oxidation bacterium enrichment device and method |
| CN103043791A (en) * | 2013-01-19 | 2013-04-17 | 桂林理工大学 | Method for lowering occurrence amount of nitrous oxide in anaerobic ammonia oxidization reactor |
| CN103145239A (en) * | 2013-03-11 | 2013-06-12 | 丽水学院 | Quick enriching reaction equipment for anammox |
| CN103145239B (en) * | 2013-03-11 | 2014-10-29 | 丽水学院 | Quick enriching reaction equipment for anammox |
| CN104556410A (en) * | 2015-01-09 | 2015-04-29 | 浙江大学 | Marine ammonia-oxidizing archaea enrichment device capable of automatically controlling dissolved oxygen |
| CN109502746A (en) * | 2018-11-26 | 2019-03-22 | 中广核环保产业有限公司 | Sewage treatment process based on Anammox |
| CN115259556A (en) * | 2022-07-26 | 2022-11-01 | 上海净豚环保科技有限公司 | Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater |
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| CN101195803B (en) | 2011-07-27 |
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Granted publication date: 20110727 Termination date: 20111225 |