CN115259556A - Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater - Google Patents
Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater Download PDFInfo
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- 239000002351 wastewater Substances 0.000 title claims abstract description 89
- 239000010802 sludge Substances 0.000 title claims abstract description 63
- 230000035755 proliferation Effects 0.000 title claims abstract description 30
- 238000012364 cultivation method Methods 0.000 title claims description 12
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 21
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 238000009630 liquid culture Methods 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- 229920002401 polyacrylamide Polymers 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000001035 drying Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 15
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 239000011780 sodium chloride Substances 0.000 claims description 12
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 12
- 239000012621 metal-organic framework Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000004537 pulping Methods 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000005189 flocculation Methods 0.000 claims description 9
- 230000016615 flocculation Effects 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- LXBGSDVWAMZHDD-UHFFFAOYSA-N 2-methyl-1h-imidazole Chemical compound CC1=NC=CN1 LXBGSDVWAMZHDD-UHFFFAOYSA-N 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 6
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 6
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 6
- 235000018417 cysteine Nutrition 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 claims description 6
- 229940025294 hemin Drugs 0.000 claims description 6
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims description 6
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims description 6
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 6
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 6
- -1 polyethylene Polymers 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 239000011592 zinc chloride Substances 0.000 claims description 6
- 235000005074 zinc chloride Nutrition 0.000 claims description 6
- 229920001131 Pulp (paper) Polymers 0.000 claims description 5
- 238000010411 cooking Methods 0.000 claims description 4
- 239000002699 waste material Substances 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- 230000002062 proliferating effect Effects 0.000 claims 5
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000000463 material Substances 0.000 claims 1
- 239000002114 nanocomposite Substances 0.000 claims 1
- 238000012136 culture method Methods 0.000 abstract description 7
- 238000005202 decontamination Methods 0.000 abstract description 6
- 230000003588 decontaminative effect Effects 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 238000004065 wastewater treatment Methods 0.000 description 4
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010000 carbonizing Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000002289 effect on microbe Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 230000002650 habitual effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
- C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater, and relates to the technical field of anaerobic granular sludge. When the proliferation culture of the anaerobic granular sludge for the papermaking wastewater is carried out, the papermaking wastewater is flocculated by using a polyacrylamide flocculant to obtain flocculated papermaking wastewater, a nutrient solution is added into the flocculated papermaking wastewater to carry out anaerobic treatment to obtain an anaerobic treatment solution, nitrogen-containing carbon quantum dots are added into the anaerobic treatment solution to obtain active granular sludge, and a liquid culture medium is used for culturing the active granular sludge to obtain the anaerobic granular sludge for the papermaking wastewater. The proliferation and culture method of the anaerobic granular sludge for the papermaking wastewater has excellent decontamination performance.
Description
Technical Field
The invention relates to the technical field of anaerobic granular sludge, in particular to a proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater.
Background
In China, straw, wood pulp and the like are mostly adopted as paper making raw materials. The pulping and papermaking production generally comprises the working procedures of pulping, pulp washing, bleaching and papermaking. The pulping in the paper industry includes alkaline pulping, chemi-mechanical pulping and mechanical pulping. The waste water has different generation and discharge of pollutants according to different pulping methods, different raw materials, different pulping yields, different paper making varieties and the existence of chemical recovery, but basically contains a large amount of suspended matters, BOD, COD and partial toxic substances and is treated by adopting a mode of combining a physicochemical method and a biological method.
The anaerobic digestion process is a wastewater treatment process with potential feasibility, which is successfully applied to the wastewater treatment in the fields of life, industry, agriculture and the like in recent years. Upflow Anaerobic Sludge Blanket (UASB) is one of the most competitive anaerobic digestion technologies in current wastewater treatment technologies, showing great potential in treating various kinds of wastewater with different properties, and nearly 80% of the world's anaerobic sewage treatment systems are based on the UASB technology. The UASB has the advantages of strong methane production capacity, low residual sludge yield and the like; another significant advantage of UASB is high pollutant removal efficiency, with Chemical Oxygen Demand (COD) removal rates mostly exceeding 90%. UASB is one of the most efficient and widely used anaerobic reactors, and has good settleability and biological activity.
Disclosure of Invention
The invention aims to provide a proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater, which aims to solve the problems in the prior art.
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps: flocculation treatment, anaerobic treatment, granulation and multiplication culture.
As optimization, the proliferation and culture method of the anaerobic granular sludge for the papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 20 to 24h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding a nutrient solution with the mass of 0.1 to 0.2 times of that of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the mixture by using a polyethylene film, and standing the mixture for 30 to 36h at the temperature of 30 to 40 ℃ to obtain an anaerobic treatment solution;
(3) Granulating: adding nitrogen-containing carbon quantum dots with the mass 0.03 to 0.05 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring at 30 to 40 ℃ and 300 to 500r/min for 40 to 50min, filtering, and drying at 10 to 20 ℃ and 1 to 10Pa for 6 to 8h to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 20-30 times that of the activated granular sludge, standing for 30-36h in an anaerobic environment at 30-40 ℃, filtering, and drying for 6-8h at 10-20 ℃ and 1-10Pa to prepare the anaerobic granular sludge for the papermaking wastewater.
Preferably, the papermaking wastewater in the step (1) is one or a mixture of waste liquor generated by cooking wood pulp, wastewater discharged by a beating machine and a refiner and papermaking machine wastewater.
Preferably, the solvent of the nutrient solution in the step (2) is water, and the composition comprises 20 to 30g/L glucose, 7 to 9g/L magnesium sulfate, 6 to 8g/L calcium chloride, 1 to 2g/L cobalt chloride, 1 to 2g/L ferric chloride, 0.03 to 0.05g/L zinc chloride, 0.08 to 0.1g/sodium chloride, 0.03 to 0.05g/L nickel chloride and 0.2 to 0.3mL/L hydrogen chloride.
As an optimization, the preparation method of the nitrogen-containing carbon quantum dot in the step (3) comprises the following steps: preheating a metal organic framework at 100-120 ℃ for 30-40min in a nitrogen atmosphere, raising the temperature to 250-300 ℃ at the speed of 5-6 ℃/min, standing for 60-80min, raising the temperature to 800-900 ℃, and standing for 2-3h to obtain the nitrogen-containing carbon quantum dot.
Preferably, the liquid medium solvent in the step (4) is water, and the components of the liquid medium solvent comprise 20% of polypeptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Preferably, the anaerobic granular sludge for papermaking wastewater prepared by the proliferation and cultivation method of the anaerobic granular sludge for papermaking wastewater is applied to pollution treatment of waste liquid generated by cooking wood pulp, wastewater discharged by a pulping machine and a refiner and papermaking machine wastewater.
Compared with the prior art, the invention has the following beneficial effects:
the invention carries out flocculation treatment, anaerobic treatment, granulation and enrichment culture in turn when the proliferation culture of the anaerobic granular sludge for the papermaking wastewater is carried out.
Firstly, flocculating treatment is carried out to remove suspended particles in the papermaking wastewater, anaerobic treatment is carried out to propagate anaerobic microorganisms in the residual papermaking wastewater, and targeted microorganism culture is carried out on the papermaking wastewater treatment, so that the microorganisms have good targeted decontamination on the papermaking wastewater.
Secondly, the nitrogen-containing carbon quantum dots obtained by carbonizing the zinc nitrate and the 2-methylimidazole after reaction are granulated, have better adsorption loading effect on microorganisms in the water body, and enable the microorganisms to proliferate on the nitrogen-containing carbon quantum dots, so that the anaerobic granular sludge for the papermaking wastewater has good decontamination effect; and (4) proliferation culture, wherein the liquid culture medium contains a large amount of nutrients, so that the proliferation of microorganisms is promoted, and the using effect is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to more clearly illustrate the method provided by the invention, the following examples are used for detailed description, and the method for testing each index of the proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater prepared in the following examples is as follows:
decontamination performance: the proliferation and cultivation method of the anaerobic granular sludge for the papermaking wastewater obtained in each embodiment and a comparative product take the same mass, the anaerobic granular sludge and the comparative product are placed in the same kind of papermaking wastewater with the same light absorption rate, the light absorption rate is measured again after the same time, and the habitual light absorption rate reduction rate is calculated.
Example 1
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, and naturally standing for 20 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.1 time of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 36 hours at 30 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water and comprises 20g/L glucose, 7g/L magnesium sulfate, 6g/L calcium chloride, 1g/L cobalt chloride, 1g/L ferric chloride, 0.03g/L zinc chloride, 0.08 g/L sodium chloride, 0.03g/L nickel chloride and 0.2mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4:10:100, uniformly mixing, stirring and reacting for 5 hours at 30 ℃ and 300r/min, centrifugally separating, washing for 3 times by using absolute ethyl alcohol, and drying for 8 hours at 10 ℃ and 1Pa to prepare a metal organic framework; preheating a metal organic framework at 100 ℃ for 40min in a nitrogen atmosphere, heating to 250 ℃ at the speed of 5 ℃/min, standing for 80min, heating to 800 ℃, and standing for 3h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.03 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 50min at 30 ℃ and 300r/min, filtering, and drying for 8h at 10 ℃ and 1Pa to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass 20 times that of the activated granular sludge, standing for 36h in an anaerobic environment at 30 ℃, filtering, and drying for 8h at 10 ℃ under 1Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Example 2
A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, and naturally standing for 22 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.15 times of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 33 hours at 35 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water, and the nutrient solution comprises 25g/L glucose, 8g/L magnesium sulfate, 7g/L calcium chloride, 1.5g/L cobalt chloride, 1.5g/L ferric chloride, 0.04g/L zinc chloride, 0.09 g/sodium chloride, 0.04g/L nickel chloride and 0.25mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4.5:15:110, stirring and reacting for 4 hours at 35 ℃ and 400r/min, centrifugally separating, washing for 4 times by using absolute ethyl alcohol, and drying for 7 hours at 15 ℃ and 5Pa to prepare a metal organic framework; preheating a metal organic framework at 110 ℃ for 35min in a nitrogen atmosphere, heating to 280 ℃ at the speed of 5.5 ℃/min, standing for 70min, heating to 850 ℃, and standing for 2.5h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.04 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 45min at 35 ℃ and 400r/min, filtering, and drying for 7h at 15 ℃ and 5Pa to obtain active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 25 times that of the activated granular sludge, standing for 33h at 35 ℃ in an anaerobic environment, filtering, and drying for 7h at 15 ℃ under 5Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Example 3
A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 24 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.2 time of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 30 hours at 40 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water and comprises 30g/L glucose, 9g/L magnesium sulfate, 8g/L calcium chloride, 2g/L cobalt chloride, 2g/L ferric chloride, 0.05g/L zinc chloride, 0.1 g/L sodium chloride, 0.05g/L nickel chloride and 0.3mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:5:20:120, stirring and reacting for 3 hours at 40 ℃ and 500r/min, centrifugally separating, washing for 5 times by using absolute ethyl alcohol, and drying for 6 hours at 20 ℃ and 10Pa to obtain a metal organic framework; preheating a metal organic framework at 120 ℃ for 30min in a nitrogen atmosphere, heating to 300 ℃ at the speed of 6 ℃/min, standing for 60min, heating to 900 ℃, and standing for 2h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.05 time of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 40min at 40 ℃ and 500r/min, filtering, and drying for 6h at 20 ℃ and 10Pa to obtain active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass 30 times that of the activated granular sludge, standing for 30h at 40 ℃ in an anaerobic environment, filtering, and drying for 6h at 20 ℃ under 10Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Comparative example 1
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 22h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.15 times of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 33 hours at 35 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water, and the nutrient solution comprises 25g/L glucose, 8g/L magnesium sulfate, 7g/L calcium chloride, 1.5g/L cobalt chloride, 1.5g/L ferric chloride, 0.04g/L zinc chloride, 0.09 g/L sodium chloride, 0.04g/L nickel chloride and 0.25mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, terephthalic acid, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4.5:15:110, stirring and reacting for 4 hours at 35 ℃ and 400r/min, centrifugally separating, washing for 4 times by using absolute ethyl alcohol, and drying for 7 hours at 15 ℃ and 5Pa to prepare a metal organic framework; preheating a metal organic framework at 110 ℃ for 35min in a nitrogen atmosphere, heating to 280 ℃ at the speed of 5.5 ℃/min, standing for 70min, heating to 850 ℃, and standing for 2.5h to obtain nitrogen-containing carbon quantum dots; adding carbon quantum dots with the mass 0.04 time of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 45min at 35 ℃ and 400r/min, filtering, and drying for 7h at 15 ℃ and 5Pa to obtain activated granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 25 times that of the activated granular sludge, standing for 33h at 35 ℃ in an anaerobic environment, filtering, and drying for 7h at 15 ℃ under 5Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Examples of effects
The following table 2 shows the results of performance analysis of the decontamination performance of the anaerobic granular sludge for papermaking wastewater prepared by the proliferation culture method of the anaerobic granular sludge for papermaking wastewater in examples 1 to 3 of the present invention and in comparative example 1:
| rate of decrease in absorption | Rate of decrease in absorption | ||
| Example 1 | 18.5% | Example 3 | 18.9% |
| Example 2 | 18.8% | Comparative example 1 | 7.2% |
TABLE 1
From the comparison of the experimental data of examples 1, 2 and 3 and comparative example 1 in table 1, it can be seen that the light absorption reduction rate of examples 1, 2 and 3 is high compared with that of comparative example 1, which indicates that the nitrogen-containing carbon quantum dots obtained by carbonizing the zinc nitrate and 2-methylimidazole after reaction have a better adsorption loading effect on microorganisms in the water body and allow the microorganisms to proliferate on the nitrogen-containing carbon quantum dots, so that the anaerobic granular sludge for papermaking wastewater has a good decontamination effect.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps: flocculation treatment, anaerobic treatment, granulation and proliferation culture;
mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 20 to 24h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding a nutrient solution with the mass of 0.1 to 0.2 times of that of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the mixture by using a polyethylene film, and standing the mixture for 30 to 36h at the temperature of 30 to 40 ℃ to obtain an anaerobic treatment solution;
(3) Granulating: adding nitrogen-containing carbon quantum dots with the mass 0.03 to 0.05 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring at 30 to 40 ℃ and 300 to 500r/min for 40 to 50min, filtering, and drying at 10 to 20 ℃ and 1 to 10Pa for 6 to 8h to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 20-30 times that of the activated granular sludge, standing for 30-36h in an anaerobic environment at 30-40 ℃, filtering, and drying for 6-8h at 10-20 ℃ and 1-10Pa to prepare the anaerobic granular sludge for the papermaking wastewater.
2. The method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater as claimed in claim 1, wherein the papermaking wastewater in the step (1) is one or more of waste liquor generated by cooking wood pulp, wastewater discharged from a pulping machine and a refiner and wastewater discharged from a papermaking machine.
3. The method for proliferating the anaerobic granular sludge for papermaking wastewater according to claim 2, wherein a solvent of the nutrient solution in the step (2) is water, and the components of the solvent include 20 to 30g/L glucose, 7 to 9g/L magnesium sulfate, 6 to 8g/L calcium chloride, 1 to 2g/L cobalt chloride, 1 to 2g/L ferric chloride, 0.03 to 0.05g/L zinc chloride, 0.08 to 0.1g/L sodium chloride, 0.03 to 0.05g/L nickel chloride and 0.2 to 0.3mL/L hydrogen chloride.
4. The proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater according to claim 3, wherein the preparation method of the nitrogen-containing carbon quantum dots in the step (3) comprises the following steps: preheating a metal organic framework at 100 to 120 ℃ for 30 to 40min in a nitrogen atmosphere, raising the temperature to 250 to 300 ℃ at the speed of 5 to 6 ℃/min, standing for 60 to 80min, raising the temperature to 800 to 900 ℃, and standing for 2 to 3h to prepare the nitrogen-containing carbon quantum dot.
5. The proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater according to claim 4, characterized in that the preparation method of the metal organic framework comprises the following steps: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4:10:100 to 1:5:20:120, stirring and reacting for 3 to 5 hours at the temperature of 30 to 40 ℃ and at the speed of 300 to 500r/min, centrifugally separating, washing for 3 to 5 times by using absolute ethyl alcohol, and drying for 6 to 8 hours at the temperature of 10 to 20 ℃ and at the pressure of 1 to 10Pa to prepare the nano-composite material.
6. The method for proliferating and cultivating the anaerobic granular sludge for the paper-making wastewater according to claim 5, wherein the liquid culture medium solvent in the step (4) is water, and the composition of the liquid culture medium solvent comprises 20% of polypeptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
7. The method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater according to claim 6, wherein the anaerobic granular sludge for the papermaking wastewater, which is prepared by the method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater, is applied to pollution treatment of waste liquor generated by cooking wood pulp, waste water discharged from a pulping machine and a refiner and paper machine waste water.
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