CN100529049C - Magnetic bacteria fast-collection device - Google Patents
Magnetic bacteria fast-collection device Download PDFInfo
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- CN100529049C CN100529049C CN 200610051057 CN200610051057A CN100529049C CN 100529049 C CN100529049 C CN 100529049C CN 200610051057 CN200610051057 CN 200610051057 CN 200610051057 A CN200610051057 A CN 200610051057A CN 100529049 C CN100529049 C CN 100529049C
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- enrichment
- collecting bin
- cylindrical shell
- collection device
- magnetic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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Abstract
The invention discloses a rapid collecting device of magnetic bacteria, which comprises the following parts: enriched bin (1), collecting bin (2), cylinder (3) and coil (4), wherein the overflow hole (5) and enriched bin pipe (6) within filter membrane (7) are set on the top of enriched bin (1); the collecting hole (8) is set on the top of collecting bin (2), whose bottom contains collecting bin pipe (9); the control valve (10) is set on the collecting bin pipe (9); the enriched bin (1) and collecting bin (2) connect through enriched bin pipe (6) and collecting bin pipe (9); the enriched bin (1) is placed on the cribellum (11) in the cylinder (3), which is winded by coil (4).
Description
Technical field
The present invention relates to a kind of magnetic bacteria fast-collection device.
Background technology
Magnetotactic bacteria (magnetic bacteria) is the general name of a class special bacterium that can move along magnetic line of force.Magnetotactic bacteria can be used as the model animals of biomagnetism research, complex biological to the research higher organism becomes the ore deposit system, inquire into origin of life hypothesis that the Mars aerolite causes and have the most important theories researching value in order to environment indication etc., in addition, bacteriogenic magnetic corpusculum can be widely used in fields such as materialogy, biology, medical science, electronics, optics, magnetics, Pollution abatement, energy storage and electrochemistry.Therefore make up the primary prerequisite that a kind of effective magnetotactic bacteria separation method is research and application.The separation method of magnetotactic bacteria mainly is based on magnetotaxis at present and utilizes the externally-applied magnetic field separation energy to synthesize the magnetotactic bacteria of magnetic corpusculum and tool mobility, as: it is uncovered that Wei Yangbao etc. utilize the left-hand thread method to design an end, the other end side has thief hole, the centre is the cylindrical collector of a control piston, the compound sample of part enrichment is put into plate, and flood iron net in the ware, collector (sterilizing) is full of sterilized water, opening end covers two filter paper, it is online to be inverted in iron rapidly, a magnet is placed on collector bottom (S is extremely downward), collect the 3-4h closure piston, cultivate from the thief hole sampling with asepsis injector.This method required time is longer, and complicated operation, collecting effect are poor, lacks quantizating index.
Summary of the invention
The objective of the invention is to overcome above-mentioned shortcoming and provide a kind of simple in structure, efficient is high, the effective magnetic bacteria fast-collection device of the magnetotactic bacteria in concentration and separation soil or the water body.
Purpose of the present invention and solve its technical problem underlying and realize by the following technical solutions: magnetic bacteria fast-collection device of the present invention, comprise enrichment storehouse 1, collecting bin 2, cylindrical shell 3 and coil 4,1 top, enrichment storehouse is provided with overflow weir 5 and enrichment storekeeper road 6, in the enrichment storekeeper road 6 filter membrane 7 is arranged; Collecting bin 2 tops are provided with collection hole 8, and its bottom is provided with collecting bin pipeline 9, and collecting bin pipeline 9 is provided with a by-pass valve control 10; Enrichment storehouse 1 links together by enrichment storekeeper road 6 and collecting bin pipeline 9 with collecting bin 2, and enrichment storehouse 1 places on the sieve plate 11 in the cylindrical shell 3, cylindrical shell 3 outer winding arounds 4.
Above-mentioned magnetic bacteria fast-collection device, wherein sieve plate 11 is the 1/6-1/5 of cylindrical shell 3 length overalls with the distance of cylindrical shell 3 bottoms.
Above-mentioned magnetic bacteria fast-collection device, wherein there is outside screw collecting bin pipeline 9 ends, and there is internal thread 6 ends, enrichment storekeeper road.
Above-mentioned magnetic bacteria fast-collection device, wherein sieve plate 11 places on the journal stirrup 12.
Above-mentioned magnetic bacteria fast-collection device, its middle cylinder body bottom is provided with supporting leg 13.
The present invention compared with prior art, the collecting bin and the enrichment storehouse of collecting magnetotactic bacteria are installed in the magnetic coil, control the size and Orientation of external magnetic field strength by the size and Orientation of regulating electric current in the magnetic coil, and in conjunction with external automatic control system and electromagnetic induction system, accurately parameters such as the temperature of control collection system and pH value make whole device collect various magnetotactic bacteria fast with the operating parameter of optimum.Its cost is low, makes simple, easy and simple to handle; Enriching apparatus and collector are directly assembled, and have simplified operation, have improved collection effciency; Use range is wide, the multiple magnetotactic bacteria of energy separated and collected; Separation and gathering speed are fast, can shorten to 1-3 hour by this device; The collection of magnetic bacteria has realized real-time control.
The specific embodiment of the present invention is provided in detail by following examples and accompanying drawing thereof.
Description of drawings
Accompanying drawing is a structural representation of the present invention.
Mark among the figure: 1, enrichment storehouse; 2, collecting bin; 3, cylindrical shell; 4, coil; 5, overflow weir; 6, enrichment storekeeper road; 7, filter membrane; 8, collection hole; 9, collecting bin pipeline; 10, valve; 11, sieve plate; 12, journal stirrup; 13, supporting leg.
Embodiment
Below in conjunction with accompanying drawing and preferred embodiment, embodiment, structure, feature and the effect thereof of the magnetic bacteria fast-collection device that foundation the present invention is proposed, describe in detail as after.
Referring to accompanying drawing, magnetic bacteria fast-collection device, comprise enrichment storehouse 1, collecting bin 2, cylindrical shell 3 and coil 4,1 top, enrichment storehouse is provided with overflow weir 5 and enrichment storekeeper road 6, filter membrane 7 (determining the pore size of filter membrane according to the needs of collecting different magnetic bacterias) is arranged in the enrichment storekeeper road 6, and filter membrane 7 is in order to prevent that magnetic mineral grain or impurity from entering collecting bin; Collecting bin 2 tops are provided with collection hole 8, and its bottom is provided with collecting bin pipeline 9, and collecting bin pipeline 9 is provided with a by-pass valve control 10, plays on-off action; Enrichment storehouse 1 links together by enrichment storekeeper road 6 and collecting bin pipeline 9 with collecting bin 2, and enrichment storehouse 1 places on the sieve plate 11 in the cylindrical shell 3, and sieve plate 11 places on the journal stirrup 12, cylindrical shell 3 outer winding arounds 4.There is outside screw collecting bin pipeline 9 ends, and there is internal thread 6 ends, enrichment storekeeper road, so that connect.Sieve plate 11 is the 1/6-1/5 of cylindrical shell 3 length overalls with the distance of cylindrical shell 3 bottoms.The cylindrical shell bottom is provided with supporting leg 13.
During use, come the enrichment culture magnetic bacteria with enrichment storehouse 1 earlier, to reach segregative concentration.Sieve plate 11 is placed on the intravital journal stirrup 12 of tube, in finishing sample to be separated, place one deck filter membrane 7 in the enrichment storekeeper road 6 at 1 top, enrichment storehouse of the enrichment culture process of magnetic bacteria, cooperate by the internal and external threads on enrichment storekeeper road 6 and the collecting bin pipeline 9 then enrichment storehouse 1 and collecting bin 2 are linked together, in sterilized collecting bin 2, inject aseptic culture fluid, opening valve 10 and overflow weir 5 allows enrichment storehouse 1 be communicated with the pipeline of collecting bin 2, on the sieve plate 11 that the enrichment storehouse 1 that links together and collecting bin 2 are put into cylindrical shell 3, make coil electricity, because magnetic corpusculum chain is given its magnetic dipole moment with inducedmagnetic field in the magnetotactic bacteria body, the magnetotactic bacteria that just can make those synthesize the magnetic corpusculum and have a mobility passes the filter opening of filter membrane 7, moves about in collecting bin 2.According to the different controlled temperature of kind of magnetic bacteria, the pH value, magneticstrength, under this temperature, pH value and magneticstrength, the speed of moving about of magnetic bacteria reaches the fastest, in 1-3h, makes magnetic bacteria reach segregative quantity in collecting bin 2.Valve-off 10, from top collection hole 8 usefulness aseptic straws draw contain magnetotactic bacteria collection liquid to aseptic Erlenmeyer flask, adopt conventional bacterium separation method can isolate magnetic bacteria.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, any technical solution of the present invention content that do not break away from,, all still belong in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment did according to technical spirit of the present invention.
Claims (5)
1, a kind of magnetic bacteria fast-collection device, it is characterized in that: comprise enrichment storehouse (1), collecting bin (2), cylindrical shell (3) and coil (4), top, enrichment storehouse (1) is provided with overflow weir (5) and enrichment storekeeper road (6), and filter membrane (7) is arranged in the enrichment storekeeper road (6); Collecting bin (2) top is provided with collection hole (8), and its bottom is provided with collecting bin pipeline (9), and collecting bin pipeline (9) is provided with a by-pass valve control (10); Enrichment storehouse (1) links together by enrichment storekeeper road (6) and collecting bin pipeline (9) with collecting bin (2), and enrichment storehouse (1) places on the interior sieve plate (11) of cylindrical shell (3), the outer winding around of cylindrical shell (3) (4).
2, magnetic bacteria fast-collection device as claimed in claim 1 is characterized in that: sieve plate (11) is the 1/6-1/5 of cylindrical shell (3) length overall with the distance of cylindrical shell (3) bottom.
3, magnetic bacteria fast-collection device as claimed in claim 1 or 2 is characterized in that: there is outside screw collecting bin pipeline (9) end, and there is internal thread end, enrichment storekeeper road (6).
4, magnetic bacteria fast-collection device as claimed in claim 3 is characterized in that: sieve plate (11) places on the journal stirrup (12).
5, magnetic bacteria fast-collection device as claimed in claim 4 is characterized in that: the cylindrical shell bottom is provided with supporting leg (13)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610051057 CN100529049C (en) | 2006-05-12 | 2006-05-12 | Magnetic bacteria fast-collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610051057 CN100529049C (en) | 2006-05-12 | 2006-05-12 | Magnetic bacteria fast-collection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1880441A CN1880441A (en) | 2006-12-20 |
| CN100529049C true CN100529049C (en) | 2009-08-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200610051057 Expired - Fee Related CN100529049C (en) | 2006-05-12 | 2006-05-12 | Magnetic bacteria fast-collection device |
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| CN (1) | CN100529049C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195803B (en) * | 2007-12-25 | 2011-07-27 | 浙江大学 | Anaerobic ammonoxidation bacterium concentrating device |
| CN105127195B (en) * | 2015-09-30 | 2017-11-07 | 宁夏锐盛明杰知识产权咨询有限公司 | A kind of repairing method of microorganism of heavy-metal contaminated soil |
| CN106967603B (en) * | 2017-04-11 | 2019-12-10 | 苏州理合文科技有限公司 | Magnetotactic bacteria sampling culture instrument |
| CN108441422A (en) * | 2018-04-08 | 2018-08-24 | 郭庆 | A kind of soil bacteria screening system |
| CN114604969B (en) * | 2022-04-11 | 2022-12-20 | 中国石油天然气股份有限公司长庆油田分公司第五采油厂 | Anaerobic ammonium oxidation bacterium enrichment bioreactor |
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- 2006-05-12 CN CN 200610051057 patent/CN100529049C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1880441A (en) | 2006-12-20 |
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Granted publication date: 20090819 Termination date: 20130512 |