CN101721533B - Traditional Chinese medicine composition and application thereof in preparing medicine for preventing and treating porcine pseudorabies - Google Patents
Traditional Chinese medicine composition and application thereof in preparing medicine for preventing and treating porcine pseudorabies Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition and an application thereof in preparing a medicine for preventing and treating porcine pseudorabies. The traditional Chinese medicine composition comprises 6-9 random Chinese herbal medicines of elephantopus scaber, echinacea, Chinese lobelia, radix isatidis, wild chrysanthemum flower, groundsel, weeping forsythia, cordate houttuynia or astragalus root, wherein the weight ratio of various Chinese herbal medicines of the elephantopus scaber to the echinacea to the Chinese lobelia to the radix isatidis to the wild chrysanthemum flower to the groundsel to the weeping forsythia to the cordate houttuynia to the astragalus root is 30:18:26:20:18:26:20:30. Under the guide of the basic theory of the traditional Chinese medicine, the invention screens out a traditional Chinese medicine composition by combining literature search and clinical experience. The Chinese herbal medicines in the composition respectively have the function of suppressing the multiplication of porcine pseudorabies viruses and have the functions of resisting the adsorption of the porcine pseudorabies viruses and directly killing the porcine pseudorabies viruses. The composition can enhance the immunity and the oxidation resistance of a rat, so that the traditional Chinese medicine composition can be used for preventing and treating the porcine pseudorabies and has extensive market prospect.
Description
Technical field
The present invention relates to field of veterinary, be specifically related to pig industry, relate in particular to a kind of Chinese medicine composition and the application in preparation control pseudorabies medicine thereof.
Background technology
Porcine pseudorabies is that a kind of (Psendorabies virus, the acute infectious disease that PRV) causes can cause in-pig miscarriage, stillborn fetus and respiratory symptom by PRV.This disease has fast, the characteristics such as high, the popular scope of mortality rate is wide, the route of transmission is many, pathogen stubbornness propagated in swinery, caused massive losses to pig industry every year.
The medicine research and development of viral disease are slower, mainly due to virus strict parasitics arranged, and are close with cell relation, behind the virus infected cell, get in the cell, in host cell, utilize the cellular enzymes system copies; Some viral nucleic acid is integrated in cell chromosome, is difficult to remove.The virus strictness colonizes in the cell, and making also has infringement to body cell when medicine kills and wounds virus.Existing antiviral chemicals is promptly because the too big or chemical sproof generation of toxicity, and it is clinical to be difficult to obtain gratifying effect.Many target spots mechanism of action of Chinese medicine, low toxic and side effects, advantage such as have no drug resistance make it show tempting application prospect, and the anti virus herb of screening high-efficiency low-toxicity is one of current antiviral research focus.
The pseudorabies of pig is at present main to adopt vaccine to prevent, and lacks treatment and prophylactic agent, in case the morbidity loss is huge, designs corresponding treatment by Chinese herbs medicine to PRV, and the treatment of porcine pseudorabies is significant.
At present; The antiviral mechanism of relevant Chinese medicine is not studied clear as yet fully, but significant feature comprises: direct repression, raise immunity effect, repair tissue damage, prevent and treat complication and secondary infection, actuator complete machine body relief of symptoms, prevent stress or the like.Wherein, direct repression stops absorption, the interior inhibition propagation of cell and anti-expression to infect three aspects again before comprising intrusion.Raise immunity comprises promotion humoral immunization and cellular immunization, promotes immune organ to grow, strengthens hematid immunity function, inducement interferon and enhanced NK cytoactive, and then kills pathogen.
At present, the domestic research and the effective ingredient of screening anti virus herb or the main research means of effective part group (like the element of the first species of water solublity or pure dissolubility or liposoluble dissolubility) are the cell in vitro methods.External antiviral screening study mainly is extraction, a separating effective ingredient from natural biological; And adopt multiple molecular biology research means (especially with the relevant method of modern immunological experiment research) to study the relation between its molecular structure and antiviral molecule mechanism; Thereby seek effective antiviral drugs, this point is at present domestic still to be weak.Simultaneously because viral species is various, simple in structure, variation is complicated; Drug metabolism processes was complicated after Chinese medicine got into body; Add that Chinese medicine gets into behind the body the existence of the many factors such as conspiracy relation between immune influence and antiviral, this just makes that to the suitable treatment by Chinese herbs medicine of a certain virus design be a complicacy and difficult work.
Summary of the invention
The objective of the invention is to deficiency, a kind of Chinese medicine composition of preventing and treating porcine pseudorabies is provided to prior art.
Another object of the present invention is to provide the application of above-mentioned Chinese medicine composition in preparation control pseudorabies medicine.
Above-mentioned purpose of the present invention is achieved through following scheme:
The inventor at first selects the Radix Astragali, Radix Isatidis, Herba Portulacae Grandiflorae, Herba Senecionis Scandentis, Flos Lonicerae, Folium Isatidis, Fructus Forsythiae, Herba Moslae, Herba Houttuyniae, Elephantopus scaber L., Flos Chrysanthemi Indici, Rhizoma Osmundae, Venenum Bufonis, Borneolum Syntheticum and these 15 kinds of Chinese medicines of Radix Scutellariae as object of study according to PRV; Efficacy of medicine observing through 15 kinds of external anti-Pseudorabies viruses of single medicinal material; And mice in vivo test; Study of the influence of various compound preparations to mouse immune power and oxidation resistance; Thereby filter out a kind of Chinese medicine composition, this Chinese medicine composition is formed by any 6～9 kinds in these 9 kinds of Chinese herbal medicine of Elephantopus scaber L., Echinacea, Herba Scutellariae Barbatae, Radix Isatidis, Flos Chrysanthemi Indici, Herba Senecionis Scandentis, Fructus Forsythiae, Herba Houttuyniae and the Radix Astragali.
In the above-mentioned Chinese medicine composition, the amount ratio of 9 kinds of Chinese herbal medicine is: Elephantopus scaber L.: Echinacea: Herba Scutellariae Barbatae: Radix Isatidis: Flos Chrysanthemi Indici: Herba Senecionis Scandentis: Fructus Forsythiae: Herba Houttuyniae: the Radix Astragali=30: 18: 26: 20: 18: 26: 20: 30, said amount ratio was a weight ratio.
The method for using of Chinese medicine composition of the present invention is: from these 9 kinds of Chinese herbal medicine of Elephantopus scaber L., Echinacea, Herba Scutellariae Barbatae, Radix Isatidis, Flos Chrysanthemi Indici, Herba Senecionis Scandentis, Fructus Forsythiae, Herba Houttuyniae and the Radix Astragali, choose at random any 6～9 kinds; According to aforesaid amount ratio take by weighing the back admixed together, can obtain Chinese medicine composition required for the present invention.
In the Chinese medicine composition of the present invention; Fructus Forsythiae, Flos Chrysanthemi Indici, Elephantopus scaber L., Herba Houttuyniae, Herba Scutellariae Barbatae and Radix Isatidis all have the good restraining effect to PRV in intracellular propagation; The Radix Astragali, Radix Isatidis, Elephantopus scaber L., Flos Lonicerae, Herba Scutellariae Barbatae, Herba Houttuyniae, Flos Chrysanthemi Indici and Herba Senecionis Scandentis all have anti-attraction function to PRV, and Flos Lonicerae, Herba Senecionis Scandentis, Flos Chrysanthemi Indici, Herba Houttuyniae, the Radix Astragali, Radix Isatidis and Fructus Forsythiae have direct killing action to PRV; In addition; Chinese medicine composition of the present invention induces bone-marrow-derived lymphocyte to have significant short increment effect to lipopolysaccharide (LPS); The secretion of pair cell factor IFN-γ also has tangible promotion to rent, and can also keep the normal level of AST in the serum, ALT, TBIL, DBIL and these several indexs of CHOL, significantly improves these three biochemical indicators of TP, GLB and P simultaneously; Chinese medicine composition of the present invention in addition can also improve the vigor of mice serum superoxide dismutase SOD, reduces malonaldehyde MDA content.
In sum, Chinese medicine composition of the present invention can be used for preparing the medicine of preventing and treating porcine pseudorabies, is applied in the swine rearing prevention and treatment to porcine pseudorabies.
Chinese medicine composition of the present invention can be made into any one dosage form commonly used in use, like powder, granule, injection or oral agents, in feedstuff or drinking-water, orally uses by 0.5%～1%, or processes the injection intramuscular injection and use.
Chinese medicine composition of the present invention is when being prepared into powder, and its specific operation process is: 6～9 kinds of Chinese herbal medicine in the above-mentioned Chinese medicine composition are taken by weighing mixing in proportion, pulverize the back and cross 80～100 mesh sieves, process powder.
Chinese medicine composition of the present invention is when being prepared into granule, and its specific operation process is: 6～9 kinds of Chinese herbal medicine in the above-mentioned Chinese medicine composition are taken by weighing mixing in proportion, adopt the method for water boiling concentration then, process granule.
Chinese medicine composition of the present invention is when being prepared into injection; Its specific operation process is: 6～9 kinds of Chinese herbal medicine in the above-mentioned Chinese medicine composition are taken by weighing mixing in proportion; Adopt the method for water boiling concentration filtration sterilization then, process the injection that concentration is 1g crude drug/mL.
Chinese medicine composition of the present invention is when being prepared into oral agents; Its specific operation process is: 6～9 kinds of Chinese herbal medicine in the above-mentioned Chinese medicine composition are taken by weighing mixing in proportion; Adopt the method for water boiling concentration filtration sterilization then, process the oral liquid that concentration is 1g crude drug/mL.
Compared with prior art, the present invention has following beneficial effect:
The present invention is under the guidance of Chinese medicine basic theories; In conjunction with literature search and clinical experience; Filter out a kind of Chinese medicine composition, said composition comprises Elephantopus scaber L., Echinacea, Herba Scutellariae Barbatae, Radix Isatidis, Flos Chrysanthemi Indici, Herba Senecionis Scandentis, Fructus Forsythiae, Herba Houttuyniae and the Radix Astragali, and these 9 kinds of Chinese herbal medicine are inhibited to the increment of PRV respectively; PRV had anti-adsorption and direct kill activity; And the compound recipe of these 9 kinds of Chinese herbal medicine can improve mouse immune power and oxidation resistance, so this Chinese medicine composition can be used for the control of porcine pseudorabies, has vast market prospect.
The specific embodiment
Below in conjunction with specific embodiment the present invention is done description further, but specific embodiment is not done any qualification to the present invention.
The efficacy of medicine observing of the external resisting pstudorabies poison of 1 15 kinds of single medicinal materials of embodiment
Present embodiment is selected the Radix Astragali, Radix Isatidis, Herba Portulacae Grandiflorae, Herba Senecionis Scandentis, Flos Lonicerae, Folium Isatidis, Fructus Forsythiae, Herba Moslae, Herba Houttuyniae, Elephantopus scaber L., Flos Chrysanthemi Indici, Rhizoma Osmundae, Venenum Bufonis, Borneolum Syntheticum and 15 kinds of Chinese medicines of Radix Scutellariae, these 15 kinds of single medicinal materials is carried out the efficacy of medicine observing of external resisting pstudorabies poison.
One, the research of single medicinal material
1.15 plant the extracting solution preparation of single medicinal material
Rhizoma Osmundae extract: Rhizoma Osmundae coarse powder 50g warp 80% ethanol heating and refluxing extraction 2 times, each 10 times of volumes, 40min, extracting solution filter the back while hot and merge, decompression rotary evaporation crude extract, standardize solution is to 50ml (1: 1, volume ratio) alcohol extract.
The Venenum Bufonis extracting solution: Venenum Bufonis 1g is through 80% ethanol heating extraction, and pure volume is 200 times of medical material, decompression rotary evaporation extracting solution, and standardize solution is to 100ml (1: 100, volume ratio) alcohol extract.
The Borneolum Syntheticum extracting solution: Borneolum Syntheticum 1g extracts through the dissolving of 80% ethanol, is settled to 100ml (1: 100, volume ratio) alcohol extract.
The Radix Scutellariae extracting solution: Radix Scutellariae coarse powder 50g water is carried, decocting twice, and the 30min that at every turn seethes with excitement merges decocting liquid twice, and normal pressure vacuum filtration and concentrated transfers PH=7.2 to be settled to 50ml (1: 1, volume ratio) decocting liquid.
The Radix Astragali, Radix Isatidis, Herba Portulacae Grandiflorae, Herba Senecionis Scandentis, Flos Lonicerae, Folium Isatidis, Fructus Forsythiae, Herba Moslae, Herba Houttuyniae, Elephantopus scaber L., Flos Chrysanthemi Indici extracting solution: each medicine is slightly carried by traditional decocting method of boiling, and said medicine all is crushed to 20 orders, soaks 2h; Decocting twice boils 30min at every turn, merges decocting liquid twice; Normal pressure vacuum filtration and concentrated; Be settled to 50ml (1: 1, volume ratio) decocting liquid, be equivalent to 1g crude drug/ml.
2. the mensuration result of Chinese medicine pair cell half toxic concentration
Through the cell survival situation behind the mtt assay detection adding Chinese medicine, judge indirectly that according to calculating average OD value the toxicity of Chinese medicine is big or small, like table 1, shown in 2 and 3.
Can know by table 1, toxicity by little to being arranged as greatly: the Radix Astragali＜Herba Moslae＜Radix Isatidis＜Herba Senecionis Scandentis＜Fructus Forsythiae＜Herba Scutellariae Barbatae, Elephantopus scaber L.＜Folium Isatidis＜Herba Houttuyniae＜Flos Chrysanthemi Indici＜Radix Scutellariae＜Flos Lonicerae＜Rhizoma Osmundae＜Borneolum Syntheticum＜Venenum Bufonis.
In experimentation, observing Chinese medicine also has certain toxic action to the PK cell, and its toxic action shows as mainly that the cell refractivity increases, becomes circle, adhesion, fragmentation, comes off, death, light absorption value obviously descend.The rising of liquor strength, pair cell toxicity is big more, and survivaling cell is few more, and it is few more that MTT is reduced into the crystallization of blue first hairpin by the living cells mitochondrial dehydrogenase, and the OD value is more little, and cell survival rate is low more.
3.PRV TCID
50Measure the result
Mtt assay is measured the growth inhibition test of different dilution factor porcine pseudorabies virus PRV to the PK cell.Calculate viral growth inhibition ratio to the PK cell under each dilution factor by the OD value, the result is as shown in table 4.Get the TCID of PRV according to Reed-Muench Liang Shi method
50=10
-4.16/ 0.1ml.
Calculate according to formula: distance proportion=(being higher than 50% percent-50)/(be higher than 50% percent-be lower than 50% percent)=(57.07-50)/(57.07-12.34)=0.16.
LgTCID
50Between=distance proportion * dilution factor logarithm poor+be higher than logarithm=0.158 * (1)+(4)=-4.16 of 50% inhibitory rate of cell growth.
TCID
50＝10
-4.16/0.1ml。
4. Chinese medicine is to the inhibitory action of virus replication
Can know by table 5 and table 6; It is best in the effect of cell internal breeding that Fructus Forsythiae suppresses PRV; Next is Flos Chrysanthemi Indici, Elephantopus scaber L., Flos Lonicerae, Herba Houttuyniae, Herba Moslae, Herba Scutellariae Barbatae and Radix Isatidis; Borneolum Syntheticum, Radix Scutellariae and Venenum Bufonis take second place, and the Rhizoma Osmundae and the Radix Astragali suppress DeGrain, and Folium Isatidis and Herba Senecionis Scandentis do not have inhibitory action.
Table 6 Chinese medicine is to the therapeutical effect therapeutic index of PRV
5. Chinese medicine is to the anti-adsorption of virus
Shown in table 7 and table 8; The Radix Astragali, Radix Isatidis, Venenum Bufonis, Elephantopus scaber L., Flos Lonicerae, Herba Scutellariae Barbatae, Herba Houttuyniae, Folium Isatidis, Flos Chrysanthemi Indici, Herba Senecionis Scandentis and Herba Moslae all have anti-attraction function to PRV; Wherein best with Radix Astragali effect; Radix Isatidis, Venenum Bufonis, Elephantopus scaber L. and Flos Lonicerae etc. take second place, the Herba Moslae effect a little less than, Borneolum Syntheticum, Fructus Forsythiae, Radix Scutellariae and Rhizoma Osmundae be the effect of anti-absorption.
Table 7 Chinese medicine is to the preventive effect suppression ratio %
of PRV
| Chinese medicine | C1 | C2 | C3 | C4 | C5 |
| Toad | -4.03±12.89 | -18.01±10.08 | 38.05±17.75 | 73.69±18.74 | 40.71±5.84 |
| Plate | 104.79±6.19 | 93.07±10.88 | 43.40±3.51 | 24.34±4.08 | 15.59±4.01 |
| Gold | 109.44±8.13 | 114.56±12.32 | 97.65±7.58 | 87.04±3.63 | 53.26±1.93 |
| Ice | 90.75±4.76 | 82.04±7.57 | 55.63±10.17 | 42.76±10.03 | 14.75±4.53 |
| Stilbene | 111.65±8.37 | 94.07±7.53 | 51.68±6.42 | 9.55±6.36 | 4.92±4.09 |
| Connect | 43.21±6.04 | 89.03±5.81 | 13.34±4.90 | 10.86±4.50 | 3.59±2.62 |
| Wild | 63.35±4.86 | 97.39±3.24 | 84.79±3.90 | 46.67±2.48 | 25.52±9.02 |
| Greatly | 104.38±7.72 | 97.42±3.66 | 23.73±7.25 | 10.09±11.36 | 10.11±4.18 |
| Core | -15.31±9.51 | 4.61±7.18 | 6.70±4.94 | 17.64±10.17 | 6.12±6.83 |
| Ground | 50.00±3.00 | 62.80±5.33 | 85.87±6.84 | 64.34±9.82 | 41.33±4.34 |
| Fragrant | 78.70±12.28 | 116.48±9.27 | 101.19±8.15 | 49.39±4.07 | 30.33±7.92 |
| Fish | 80.65±14.82 | 87.32±7.72 | 67.93±11.78 | 44.07±2.86 | 40.73±5.29 |
| Half | 89.15±12.12 | 88.79±11.05 | 60.33±16.14 | 90.87±3.08 | 42.41±4.82 |
| Thousand | -3.55±1.02 | -9.71±5.63 | 58.51±7.93 | 69.40±4.56 | 43.33±6.09 |
| Pass through | -10.52±12.34 | -12.79±19.13 | -10.27±6.71 | -13.74±17.51 | -15.37±19.75 |
In the table 7, C1, C2, C3, C4, C5 represent to begin from TC50 five concentration of doubling dilution.
Table 8 Chinese medicine is to the preventive effect therapeutic index of PRV
6. Chinese medicine is to the direct deactivation of virus
Can know by table 9 and 10; Better to Venenum Bufonis, Flos Lonicerae and Rhizoma Osmundae effect in the direct deactivation of PRV virus; Wherein best with the Venenum Bufonis effect; Herba Senecionis Scandentis, Flos Chrysanthemi Indici, Herba Moslae, Herba Houttuyniae, the Radix Astragali, Radix Isatidis, Folium Isatidis and Fructus Forsythiae also have direct killing action, but effect is not as Venenum Bufonis and Flos Lonicerae ideal; The direct killing action of Borneolum Syntheticum a little less than, almost the same with Radix Scutellariae and Elephantopus scaber L., the effect that PRV is not directly killed.
Table 10 Chinese medicine is to the therapeutic index of the direct deactivation of PRV
Two, compound preparation is to the influence of mouse immune power and oxidation resistance
Experiment in vitro result according to aforementioned 15 kinds of single medicinal materials carries out the mice in vivo test, is intended to study the mechanism of Chinese medicine antivirus action.
The prescription of compound recipe I is: Flos Lonicerae 30 grams, the Radix Astragali 18 grams, Elephantopus scaber L. 20 grams, Flos Chrysanthemi Indici 12 grams, Rhizoma Osmundae 20 grams, Borneolum Syntheticum 1 gram;
The prescription of compound recipe II is: Radix Scutellariae 30 grams, Flos Lonicerae 28 grams, Fructus Forsythiae 30 grams, Folium Isatidis 20 grams, Herba Houttuyniae 16 grams, Flos Chrysanthemi Indici 12 grams, Rhizoma Osmundae 20 grams;
The prescription of compound recipe III is: Elephantopus scaber L. 30 grams, Echinacea 30 grams, Herba Scutellariae Barbatae 18 grams, Radix Isatidis 26 grams, Flos Chrysanthemi Indici 20 grams, Herba Senecionis Scandentis 18 grams, Fructus Forsythiae 26 grams, Herba Houttuyniae 20 grams and the Radix Astragali 30 grams;
The prescription of compound recipe IV is: Folium Isatidis 20 grams, Herba Houttuyniae 16 grams, Flos Chrysanthemi Indici 12 grams, Rhizoma Osmundae 20 grams.
With normal saline as matched group.
1. compound preparation is to the result of mouse spleen index influence
Can know by table 11; Compare with matched group; Four kinds of compound preparations all have certain influence to the mouse spleen index; The index and spleen index of compound recipe I, compound recipe III and compound recipe IV all is higher than matched group, and compound recipe III and compound recipe IV and the equal difference of other group contrast is (P＜0.05) significantly, and is especially outstanding with compound recipe III group difference.
Table 11 compound preparation is to the exponential influence of mouse spleen
In the above-mentioned table 11, the subscript of each numerical value of index and spleen index place letter different table differential different significantly (P＜0.05).
2. compound preparation is to the result of mice spleen lymphocytes proliferation
Can be known by table 12, compare with matched group that compound recipe I, II, III, IV are to the inductive T lymphocyte of ConA, inductive bone-marrow-derived lymphocyte of LPS and cell matched group all have short proliferation function (P＜0.05) clearly; Compound recipe IV is to the short proliferation function of inductive T lymphocyte of ConA and cell matched group especially obviously (P＜0.05); Compound recipe III then induces the short proliferation function of bone-marrow-derived lymphocyte especially to give prominence to (P＜0.05) to LPS.
In the above-mentioned table 12, the subscript of each numerical value of index and spleen index place letter different table differential different significantly (P＜0.05).
3. compound preparation is to the influence of mice serum IFN-γ
Can know by table 13, compare that the content of compound recipe III, IV group mice serum IFN-γ is higher, and significant difference (P＜0.05) with matched group.Compare with compound recipe IV group, compound recipe III group promotes that the excretory effect of cytokine IFN-γ is more obvious, significant difference (P＜0.05).
Table 13 compound preparation is to the influence
of mice serum IFN-γ
In the above-mentioned table 13, the subscript of each numerical value of concentration place of IFN-γ letter different table differential different significantly (P＜0.05).
4. the mensuration result of blood parameters
Can know that by table 14 compare with irritating the normal saline group, compound recipe I group has 5 (AST, ALT, DBIL, TG, Ca) biochemical indicators to change and significant difference (P＜0.05); Compound recipe II group has 7 (AST, TP, GLB, TBIL, DBIL, TG, Ca) biochemical indicators to raise and significant difference (P＜0.05); Compound recipe III group raises and significant difference (P＜0.05) 3 (TP, GLB, P) biochemical indicators; Compound recipe IV group has 6 (AST, ALT, ALB, DBIL, TG, CHOL) biochemical indicator values to raise and significant difference (P＜0.05).AST in the serum, ALT, TBIL, DBIL, CHOL obviously raise (P＜0.05), show that body has certain damage, and medicine has certain toxic action.And compound recipe III can make These parameters keep normal level, and significantly improves TP, GLB, significant difference (P＜0.05).Show that compound recipe III can improve the immunity of body to a certain extent.
Table 14 compound recipe is to the influence
of mouse blood biochemical indicator
In the above-mentioned table 14, the subscript of each numerical value letter different table differential different significantly (P＜0.05).
5. the mensuration result of oxidation resistance
5.1SOD mensuration result
Can know that by table 15 compare with other group, compound recipe III can improve the vigor of mice serum SOD, but difference is not remarkable; Other group is all low than normal value, and difference is not remarkable.
Table 15 compound preparation is to the influence
of mice serum SOD
5.2MDA mensuration result
Can be known that by table 16 compare with other each group, compound recipe IV can improve MDA content in the serum, and significant difference (P＜0.05), and the MDA content of its excess-three group and matched group is approaching, compound recipe III can also reduce MDA content, but difference is not remarkable.
Table 16 compound recipe is to the influence
of mice serum MDA
In the above-mentioned table 16, the subscript of each numerical value of MDA content place letter different table differential different significantly (P＜0.05).
Through research to four compound recipes; Finally draw Chinese medicine composition composition formula of the present invention; Promptly be made up of any 6～9 kinds of Chinese herbal medicine in Elephantopus scaber L., Echinacea, Herba Scutellariae Barbatae, Radix Isatidis, Flos Chrysanthemi Indici, Herba Senecionis Scandentis, Fructus Forsythiae, Herba Houttuyniae or the Radix Astragali, the weight ratio of each Chinese herbal medicine is an Elephantopus scaber L.: Echinacea: Herba Scutellariae Barbatae: Radix Isatidis: Flos Chrysanthemi Indici: Herba Senecionis Scandentis: Fructus Forsythiae: Herba Houttuyniae: the Radix Astragali=30: 18: 26: 20: 18: 26: 20: 30.
In each table of present embodiment, toad, plate, gold, ice, stilbene, companys, open country, greatly, a kind of reed mentioned in ancient books,, perfume, fish, partly, thousand, pass through and represent Venenum Bufonis, Radix Isatidis, Flos Lonicerae, Borneolum Syntheticum, the Radix Astragali, Fructus Forsythiae, Flos Chrysanthemi Indici, Folium Isatidis, Radix Scutellariae, Elephantopus scaber L., Herba Moslae, Herba Houttuyniae, Herba Scutellariae Barbatae, Herba Senecionis Scandentis, Rhizoma Osmundae respectively.
Claims (7)
1. Chinese medicine composition of preventing and treating porcine pseudorabies is characterized in that this Chinese medicine composition is made up of following component: Elephantopus scaber L. 30 grams, Echinacea 30 grams, Herba Scutellariae Barbatae 18 grams, Radix Isatidis 26 grams, Flos Chrysanthemi Indici 20 grams, Herba Senecionis Scandentis 18 grams, Fructus Forsythiae 26 grams, Herba Houttuyniae 20 grams and the Radix Astragali 30 grams.
2. the application of the said Chinese medicine composition of claim 1 in the medicine of preparation control porcine pseudorabies.
3. according to the said application of claim 2, the medicine that it is characterized in that said control porcine pseudorabies is powder, granule, injection or oral liquid.
4. according to the said application of claim 3, the powder agent medicine that it is characterized in that said control porcine pseudorabies is through the said Chinese medicine composition of claim 1 being taken by weighing mixing in proportion, pulverizing back 80～100 mesh sieves excessively and prepare and get.
5. according to the said application of claim 3, the granules medicine that it is characterized in that said control porcine pseudorabies is through the said Chinese medicine composition of claim 1 is taken by weighing mixing in proportion, adopts the method preparation of water boiling concentration then and gets.
6. according to the said application of claim 3; The injection medicine that it is characterized in that said control porcine pseudorabies is through the said Chinese medicine composition of claim 1 is taken by weighing mixing in proportion; Adopt the method for water boiling concentration filtration sterilization then, thereby prepare the injection that concentration is 1g crude drug/mL.
7. according to the said application of claim 3; The oral medicine liquid that it is characterized in that said control porcine pseudorabies is through the said Chinese medicine composition of claim 1 is taken by weighing mixing in proportion; Adopt the method for water boiling concentration filtration sterilization then, process the oral liquid that concentration is 1g crude drug/mL.
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| CN104435795A (en) * | 2014-09-30 | 2015-03-25 | 全椒县大地种植专业合作社 | Traditional Chinese medicine for treating porcine pseudorabies |
| CN104606302A (en) * | 2015-01-06 | 2015-05-13 | 格特生物制药（天津）有限公司 | Traditional Chinese medicine oral liquid for treating porcine pseudorabies and preparation method thereof |
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