CN100427099C - Juglans mandshurica maxim pdysaccharide preparation capable of effectively treating brain cancer and its preapring method - Google Patents
Juglans mandshurica maxim pdysaccharide preparation capable of effectively treating brain cancer and its preapring method Download PDFInfo
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- CN100427099C CN100427099C CNB2006100242769A CN200610024276A CN100427099C CN 100427099 C CN100427099 C CN 100427099C CN B2006100242769 A CNB2006100242769 A CN B2006100242769A CN 200610024276 A CN200610024276 A CN 200610024276A CN 100427099 C CN100427099 C CN 100427099C
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Abstract
The present invention relates to a manchurian walnut polyhexose preparation capable of effectively curing brain cancer and a preparation method of the manchurian walnut polyhexose preparation, belonging to the technical field of medicine. The manchurian walnut preparation of the present invention comprises the following ingredients (by weight percentage): 85 to 8% of manchurian walnut polyhexose and 15 to 2% of flavonoid materials. The preparation method has the following steps: (1) the collection and the drying of manchurian walnut materials; (2) the extraction of anti-cancer activity components of manchurian walnut, which has the following steps: the dried manchurian walnut materials are pulverized, watered, heated and insulated, clear liquid and dregs are separated, the dregs of the manchurian walnut materials is heated and boiled out by adding green water according to an identical method, the clear liquid is filtered and collected, the clear liquid is combined to be concentrated, and then, chloroform is respectively added to remove protein and lipid components with high contractibility; ethanol is added to cause the manchurian walnut polyhexose to deposit; finally, active polyhexose powder is obtained through drying, pulverization and sieving; (3) the configuration of an anticancer decoction. The present invention has the advantages of preparation of a traditional Chinese preparation which can effectively treat the brain cancer, high therapeutic effect, low toxic side effect, simple process and no three wastes pollution; the present invention is suitable for large scale commercial process.
Description
Technical field
The present invention relates to product of a kind of medical technical field and preparation method thereof, specifically, what relate to is a kind ofly can effectively treat Juglans mandshurica polysaccharide formulation of the brain cancer and preparation method thereof.
Technical background
Present stage, cancer therapy drug commonly used belonged to chemotherapeutics, substantially all was the Western medicine that is feature with pure product chemical compound.These medicines also have great lethal effect to the normal human cell when killing cancerous cell, show toxic and side effects in various degree.Therefore seek the popular domain that the little cancer therapy drug of toxic and side effects becomes a research.Natural drug, particularly plant amedica become the emphasis that domestic and international researcher is investigated.The paclitaxel that extracts from Ramulus et folium taxi cuspidatae, the vinblastine that obtains from Herba Catharanthi Rosei and vincristine, the camptothecine that obtains from Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae), isolating bilobalide is the model of Western medicine cancer therapy drug success from Semen Ginkgo.And from fungus, the complex polysaccharide composition that extracts such as mood ear fungus, Tremella, Ganoderma, hedgehog hydnum has for example then been annotated Chinese medicine anticancer future.Simultaneously owing to cried out multiple composition in the Chinese medicine, its anticancer effect is the synergism of many materials, in human body cell, have a plurality of drug targets, the synergism of the many target spots of this multicomponent can improve therapeutic effect on the one hand, simultaneously also anti-by closely questioning each other, to Normocellular lethality, reduced its toxic and side effects when offsetting each component and using separately.
Find that through literature search the root of Juglans mandshurica is among the people as the medicine extensive use in Korea, and is separable to multiple material with important physiological regulation function from Juglans mandshurica to prior art.People such as Min BS were separated to acyl group glucosides class material among four kinds of terpene substances and two in 2003 from the methanolic extract of Juglans mandshurica trunk, and prove that thereby it can suppress complement system and have antiinflammation, particularly wherein 1,2,3, the IC50 value of 6-four acidylate glucoses is 34mM, has tangible complement inhibit feature (Anti-complementactivity of constituents from the stem-bark of Juglans mandshurica (research of Juglans mandshurica extract anticomplementary activity) .Biol Pharm Bull (bio-pharmaceuticals circular) .2003Jul; 26 (7): 1042-4).Lee KS etc. were separated to naphthalene glycosides in six, two kinds of naphthane ketosides, naphthalene-carboxylic acid ester glycosides, five kinds of diaryl enanthol in 2002 from the methanolic extract of Juglans mandshurica root, wherein a plurality of chemical compounds demonstrate certain cytotoxic effect (Cytotoxicdiarylheptanoids from the roots of Juglans mandshurica (from the Juglans mandshurica root extraction separation to have a Cytotoxic diaryl enanthol class material) .J Nat Prod (natural product magazine) .2002Nov to the human cell line of canceration and lung carcinoma cell; 65 (11): 1707-8).Lee KS etc. 2005 by from the methanolic extract of Semen Juglandis Folium catalpae bungei, being separated to four kinds of Flavonoid substances, have antiinflammation (Flavonoids from the leaves of Litsea japonica and theiranti-complement activity (Flavonoid substances that from Japanese Litsea plant, is separated to and anticomplementary activity research thereof) .Phytother Res (plant treatment research) .2005 Apr thereby have the obvious suppression complement system; 19 (4): 273-6).Wang Chunling etc. are at " oncology's magazine " 2003,9 (3): writing articles the ethanol extraction that proposes Juglans mandshurica on the 144-6 can human cervical carcinoma cell be the HELA cell, can suppress telomerase activation wherein, induces its apoptosis.Above-mentioned document is the separation aspect of biological active substances that concentrates on the ethanol extract of Juglans mandshurica, still finding no the pass in further retrieving is the anticancer preparation of major physiological active component with the Juglans mandshurica polysaccharide, and the bibliographical information that effectively suppresses the growth aspect of cerebroma cell.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of and can effectively treat Juglans mandshurica polysaccharide formulation of the brain cancer and preparation method thereof.The Juglans mandshurica polysaccharide powder that the present invention makes can significantly suppress the growth of brain cancer cell, causes brain cancer cell death, and very little to normal impact cell simultaneously, method is simple.
The present invention is achieved by the following technical solutions, Juglans mandshurica polysaccharide formulation of the present invention, and its weight percentages of components is: Juglans mandshurica polysaccharide 85-98%, Flavonoid substances 15-2%.Flavonoid substances is meant that phenyl ring with two phenolic hydroxyl groups connects the general name of a series of chemical compounds that form by central three carbon units, comprises flavone, flavonol, flavanone, flavanonol etc.Juglans mandshurica polysaccharide formulation of the present invention is to be main active ingredient with the Juglans mandshurica polysaccharide, and containing the Water Soluble Compound body that the part Flavonoid substances is formed, the intravenously administrable of Western medicine cancer therapy drug relatively has that cost is low, the advantage of convenient drug administration, evident in efficacy, and toxic and side effects is little.
The preparation of Juglans mandshurica polysaccharide formulation of the present invention comprises the steps:
(1) collection of Juglans mandshurica material and drying
The Juglans mandshurica time acquired for materials gathered the position and comprises leaves, branch, trunk, root, fruit from March to November.The at first roguing of Juglans mandshurica of collecting, dry then.Drying means is included in that aeration-drying place is dried in the shade, three kinds of heating, drying and vacuum lyophilizations.Dried Juglans mandshurica material can be preserved in cold drying place and never degenerate half a year.
(2) extraction of Juglans mandshurica anticancer active constituent
Dried Juglans mandshurica material is pulverized, and adds several times water then, is heated to suitable temperature (60-100 ℃) insulation 4 hours, separates clear liquid and residue by vacuum filtration then.And then add new water heating infusion Juglans mandshurica material residue 2 times by the same way, and filter and collect clear liquid.Reject is endured the Juglans mandshurica material residue that boils through 3 times, merges 3 times and endures the collected clear liquid that boils, and carries out vacuum concentration to 5% to 20% of original volume.
(3) separation of Juglans mandshurica anticancer active constituent and reagent preparation
The chloroform that adds concentrated solution volume 20%-100% shook mixing 1-10 minute, left standstill then to make chloroform phase and water form boundary clearly in 10-30 minute.Highly hydrophobic lipid is dissolved in the chloroform phase, and the albumen of degeneration then is deposited on the boundary, collects the upper strata water.
Add 1-5 times of volume of ethanol at above-mentioned aqueous phase, concussion mixing 1-10 minute by filtering collecting precipitation, is the Juglans mandshurica polysaccharide formulation then after the drying.
The Juglans mandshurica polysaccharide formulation that the present invention makes can add and make decoction in the solvent, and concrete ratio is: every liter of solvent adding amount is 0.01 to restrain 200 gram Juglans mandshurica polysaccharide formulations.
Described solvent is water or low-concentration ethanol solution (concentration of alcohol is 0.1%-20%).
Juglans mandshurica preparation of the present invention can pass through the anticancer telomerase activation, and inducing cancer cell takes place to transfer dies, and the nuclear DNA of cancerous cell is ruptured between nucleosome, the ability of forfeiture further growth breeding.In experiment in vitro and zoopery, Juglans mandshurica preparation of the present invention shows the restriction inhibitory action to the cerebroma cell.
The present invention be with the Juglans mandshurica polysaccharide as main active, can effectively treat the Chinese medicine preparation of the brain cancer by oral administration.As a kind of composite natral medicine, main component is a polysaccharide, contains various active compositions such as a small amount of Flavonoid substances in addition, the curative effect height, and toxic and side effects is low.Medicament preparation technology is simple, three-waste free pollution, is suitable for large-scale industrial production.
Description of drawings
Fig. 1 is reagent vitro inhibition cerebroma cell effect figure of the present invention
Fig. 2 is for pressing down the cancer efficiency analysis in the reagent body of the present invention
The specific embodiment
The present invention earlier separates drying to the Juglans mandshurica extract, can accurately measure the content of its main active polysaccharide, and then is dissolved into the method for decoction with suitable solvent, can guarantee the quality of decoction and accurate dosage.Thereby stablize therapeutic effect.
One, polysaccharide component is identified
Get 100 milliliters in Juglans mandshurica preparation of the present invention, slowly add 200 milliliters of ethanol, stir while adding.By the centrifugal collecting precipitation of 5000g, promptly obtain polysaccharide component then.This component also can (CTAB pH=8) precipitates from its solution again, shows the reaction of the typical quaternary salt deposit of polysaccharide component by the bromide of the hexadecyl trimethylamine of 0.5%-20%.The ratio of this mixing polysaccharide ingredients constitute gross activity component content is 85%-98%.Uv atlas is presented between the 200-400 sodium rice has only end absorption, does not find the absworption peak of albumen about 280, therefore can conclude that this polysaccharide component does not contain albumen substantially.
Flavonoid component
Get 100 milliliters in Juglans mandshurica preparation of the present invention, add the basic lead acetate saturated aqueous solution of 10-50 milliliter, form the special xanchromatic lead salt precipitation of flavonoid substance, and then add the saturated sodium bicarbonate aqueous solution of 5-20 milliliter, fully stirred the back static 1 hour.Filter collecting precipitation, then precipitation is suspended in the 50%-95% ethanol, add the sodium sulfate deleading, form lead sulfate and flavonoids solution.Filter and collect supernatant, and the vacuum concentration drying, promptly obtain flavonoid component, account for gross activity composition 2%-15%.Uv atlas shows that two tangible absworption peaks appear in the alcoholic solution of this flavonoid component about 250 sodium rice and 340 sodium rice, correspond respectively to peak band 2 and peak band 1 in the flavone typical absorption pattern.But summit is broader smooth, illustrate to be the mixture of multiple flavone in this component, uv absorption for the uv absorption of these materials add and.
Two, active anticancer analysis
1 at brain mesenchymoma cells in vitro inhibition experiment
Adopt people's glioblastoma multiforme BT325IV type to carry out the anti-tumor experiment of Juglans mandshurica preparation of the present invention.Experimental technique adopts mtt assay.
Every hole adds BT325 cell culture fluid 200 microlitres of exponential phase on 96 porocyte culture plates, its cell density is that 105/mL. is at 37 ℃, the CO2 of 5-10%, hatched in the incubator of humidity 40%-85% 4 hours, after treating cell attachment, every hole adds the Juglans mandshurica preparation medicinal liquid of the present invention of the different diluted concentrations of 10-40 microlitre, with normal saline as blank.Continue to cultivate 2 days.100 milliliters of three solution of adding 10%; the dyeing of under former incubation conditions, spending the night; succinate dehydrogenase in the living cells can make the blue color crystalloid granule of MTT decomposition generation amass in cell and cell peripheral, and its amount is proportional, also proportional with cell viability with cell number.Under 570 nanometers, detect each hole absorbance then.Calculate cancerous cell according to following formula and suppress efficient:
Fig. 1 preparation vitro inhibition of the present invention cerebroma cell effect figure
The preparation of the present invention of 45% concentration just can reach the semilethal effect to brain mesenchymoma cell as can be seen, and the preparation of the present invention of 100% concentration can make 92% brain mesenchymoma cell forfeiture growth activity, has the obvious suppression effect.
2 utilize experiment mice to carry out the vivo antitumor experiment
Treating excess syndrome is tested mice, male and female half and half.Every Mus is injected G422 tumor tissues suspension 20 microlitres in brain, cell density is that 107/mL. is administered three times by every from inoculating the 3rd day every day in this suspension, one time 2,5,10,15,20,25 milliliters, with normal saline as blank.1 all every group of execution animals are 3 behind the self administration of medication, get tumor and weigh, by the tumour inhibiting rate of its mean value calculation medicine.
Press down the cancer efficiency analysis in Fig. 2 preparation body of the present invention
As can be seen, along with the increase of administration spine, tumor weight increases slowly, and when each dosage surpassed 8 milliliters, exemplary embodiment lock was reduced to half of matched group, demonstrates the effect of obvious suppression tumor growth.
Provide embodiment below in conjunction with technology contents of the present invention:
Example one prepares anticancer agent from leaves
The collection of Juglans mandshurica material and drying:
Gather 1 kilogram of Juglans mandshurica leaves in October.1 centimetre of wide bar is cut at first roguing then, dries in the shade in aeration-drying place, obtains 0.2 kilogram of Juglans mandshurica cured leaf.Dried Juglans mandshurica blade can be preserved in cold drying place and never degenerate half a year.
The extraction of Juglans mandshurica anticancer active constituent
Get above-mentioned Juglans mandshurica dried leaves bar 200 grams, add 2 liters in water, last heating kept fluidized state (100 ℃) 4 hours, and the vacuum filtration by 0.8 normal atmosphere separates boiling liquid clear liquid and residue then.And then add 2 premium on currency heated and boiled Juglans mandshurica material residues 2 times by the same way respectively, and filter and collect boiling liquid clear liquid.The Juglans mandshurica material residue of reject through boiling for 3 times merges 3 times and boils collected boiling liquid clear liquid (about 6 liters), heats under the vacuum of 0.9 normal atmosphere with Rotary Evaporators to be concentrated into 0.5 liter.Add 0.5 liter of chloroform, concussion mixing 5 minutes, leave standstill then made in 30 minutes chloroform mutually and water form boundary clearly.Highly hydrophobic lipid is dissolved in the chloroform phase, and the albumen of degeneration then is deposited on the boundary, collects the upper strata water.Add 0.7 liter of ethanol to aqueous phase, concussion mixing 10 minutes filters collecting precipitation then, be the Juglans mandshurica polysaccharide, be placed in 30-100 degree centigrade the vacuum desiccator dry 24 hours then, obtain the anticancer powder of plain edition Juglans mandshurica end 12 grams, yield 6% (12/200=6%).The anticancer powder of plain edition Juglans mandshurica is imitated pulverizer by ultra micro pulverize, cross 400 mesh sieves, obtain the anticancer powder of instant type Juglans mandshurica.
The configuration of anticancer decoction
Get 500 milliliters in the water that meets standard for drinking, be heated to boiling, add above-mentioned Juglans mandshurica polysaccharide powder then, active component dissolves rapidly, form final anti-brain cancer preparation, wherein the reactive powder weight percent concentration of Juglans mandshurica is 2.3% (12/ (500+12)=2.3%), and concentration of volume percent is every liter 24 gram Juglans mandshurica polysaccharide powder.
Example two prepares anticancer agent from tree root
The collection of Juglans mandshurica material and drying:
Gather 1 kilogram of Juglans mandshurica tree root in October, and become the about 0.25 square centimeter bar of sectional area, long about 2 centimetres of bar adopts 60 degree heating, dryings then, obtains 0.4 kilogram of dried Juglans mandshurica bar root.Can preserve in cold drying place and never degenerate half a year.
The extraction of Juglans mandshurica anticancer active constituent
Dried Juglans mandshurica bar root 400 grams add 4 kilograms in water then, are heated to 80 ℃ of insulations 4 hours, separate boiling liquid clear liquid and residue by 0.8 normal atmosphere vacuum filtration then.And then add 4 kg of water heating infusion Juglans mandshurica material residue 2 times by the same way, and filter and collect clear liquid.Reject merges the collected clear liquid of 3 infusions (about 12 liters) through the Juglans mandshurica material residue of 3 infusions, heats under the vacuum of 0.8 normal atmosphere with Rotary Evaporators to be concentrated to 1 liter.Add 1 liter of chloroform, concussion mixing 10 minutes, leave standstill then made in 30 minutes chloroform mutually and water form boundary clearly.Highly hydrophobic lipid is dissolved in the chloroform phase, and the albumen of degeneration then is deposited on the boundary, collects the upper strata water.Xiang Shuizhong adds 2 liters of ethanol again, and concussion mixing 10 minutes filters collecting precipitation then, be the Juglans mandshurica polysaccharide, be placed in 30-100 degree centigrade the vacuum desiccator dry 24 hours then, obtain the anticancer powder of plain edition Juglans mandshurica end 20 grams, yield 5% (20/400=5%).The anticancer powder of plain edition Juglans mandshurica is imitated pulverizer by ultra micro pulverize, cross 400 mesh sieves, obtain the anticancer powder of instant type Juglans mandshurica.
The configuration of anticancer decoction
Get 833 milliliters of 2% alcoholic solution that meet standard for drinking, be heated to boiling, reactive powder 20 grams that add above-mentioned Juglans mandshurica then, active component dissolves rapidly, form final anti-brain cancer preparation, wherein the weight percent content of Juglans mandshurica polysaccharide powder is 2.3% (20/ (833+20)=2.3%), and volume percent content is every liter of 24 gram.
Example three utilizes preparation of the present invention to improve the time-to-live of cerebroma experiment mice
Treating excess syndrome is tested 28 of mices, male and female half and half.Every Mus is injected G422 tumor tissues suspension 20 microlitres in brain, cell density is that 107/mL. is administered three times by every from inoculating the 3rd day every day in this suspension, one time 2,5,10,15,20,25 milliliters, as blank, observe its time-to-live with normal saline.
Statistics finds that the mice of blank group on average survived 13 days, and there is obvious prolongation the administration group mean survival time, and observes the situation of a routine tumor complete obiteration in 20 milliliters of administration groups.
The experiment mice time-to-live statistical result of table 1 cerebroma
Claims (9)
1. the Juglans mandshurica polysaccharide formulation that can effectively treat the brain cancer, it is characterized in that: its weight percentages of components is: Juglans mandshurica polysaccharide 85-98%, the Flavonoid substances 15-2% that extracts from Juglans mandshurica.
2. the Juglans mandshurica polysaccharide formulation that can effectively treat the brain cancer according to claim 1, it is characterized in that: described Flavonoid substances, be meant that phenyl ring with two phenolic hydroxyl groups connects the general name of a series of chemical compounds that form by central three carbon units, comprises flavone, flavonol, flavanone, flavanonol.
3. the Juglans mandshurica polysaccharide formulation that can effectively treat the brain cancer according to claim 1 is characterized in that: every Hydrargyri Oxydum Rubrum is made decoction with adding Juglans mandshurica polysaccharide formulation 0.1 gram-200 grams in the solvent.
4. the Juglans mandshurica polysaccharide formulation that can effectively treat the brain cancer according to claim 3 is characterized in that: described medicinal solvent is a water, and perhaps concentration of alcohol is the ethanol water of 0.1%-20%.
5. the preparation method that can effectively treat the Juglans mandshurica polysaccharide powder of the brain cancer is characterized in that may further comprise the steps:
1) collection of Juglans mandshurica material and drying:
The Juglans mandshurica time acquired for materials gathered the position and comprises leaves, branch, trunk, root, fruit from March to November, and the at first roguing of the Juglans mandshurica of collection is dry then;
2) extraction of Juglans mandshurica anticancer active constituent
Dried Juglans mandshurica material is pulverized, and adds several times water then, and heat tracing separates boiling liquid clear liquid and residue by vacuum filtration then; Add new water heating infusion Juglans mandshurica material residue more by the same way, and filter and collect supernatant, reject merges and endures the collected supernatant that boils through enduring the Juglans mandshurica material residue that boils, and carries out vacuum concentration to 5% to 20% of original volume;
3) separation of Juglans mandshurica anticancer active constituent and powder preparation
Add chloroform, shake mixing, leave standstill, make chloroform phase and water form boundary clearly, highly hydrophobic lipid is dissolved in the chloroform phase, and the albumen of degeneration then is deposited on the boundary, collects the upper strata water; Add ethanol at above-mentioned aqueous phase, the concussion mixing by filtering collecting precipitation, is the anticancer powder of Juglans mandshurica polysaccharide then after the drying;
Described Juglans mandshurica polysaccharide powder weight percentages of components is: Juglans mandshurica polysaccharide 85-98%, the Flavonoid substances 15-2% that extracts from Juglans mandshurica.
6, the preparation method that can effectively treat the Juglans mandshurica polysaccharide powder of the brain cancer according to claim 5 is characterized in that: in the step 1), described drying, adopt dry in the shade in aeration-drying place, heating, drying or vacuum lyophilization.
7, the preparation method that can effectively treat the Juglans mandshurica polysaccharide powder of the brain cancer according to claim 5 is characterized in that: step 2) in, heating-up temperature is 60-100 ℃, is incubated 4 hours.
8, the preparation method that can effectively treat the Juglans mandshurica polysaccharide powder of the brain cancer according to claim 5 is characterized in that: in the step 3), add the chloroform of concentrated solution volume 20%-100%, concussion mixing 1-10 minute left standstill 10-30 minute then.
9, the preparation method that can effectively treat the Juglans mandshurica polysaccharide powder of the brain cancer according to claim 5 is characterized in that: in the step 3), add 1-5 times of volume of ethanol at aqueous phase, concussion mixing 1-10 minute.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181257A (en) * | 1996-11-06 | 1998-05-13 | 王占一 | Prescription of anti-cancer agent belonging to anti-cancer Chinese herbs |
| CN1457849A (en) * | 2003-05-07 | 2003-11-26 | 徐志强 | Specific medicine for curing carcinoma of stomach |
| CN1634292A (en) * | 2004-10-29 | 2005-07-06 | 刘今方 | Chinese traditional medicine for treating liver cancer and preparation technique thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181257A (en) * | 1996-11-06 | 1998-05-13 | 王占一 | Prescription of anti-cancer agent belonging to anti-cancer Chinese herbs |
| CN1457849A (en) * | 2003-05-07 | 2003-11-26 | 徐志强 | Specific medicine for curing carcinoma of stomach |
| CN1634292A (en) * | 2004-10-29 | 2005-07-06 | 刘今方 | Chinese traditional medicine for treating liver cancer and preparation technique thereof |
Non-Patent Citations (2)
| Title |
|---|
| 青龙衣两提取部位均有抗肿瘤作用. 季宇彬，马宏图，杨波.中国医药报. 2005 |
| 青龙衣两提取部位均有抗肿瘤作用. 季宇彬，马宏图，杨波.中国医药报. 2005 * |
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