CN101717716B - Automatic chain-type solid-state fermentation reaction device and method of automatic solid-state fermentation thereof - Google Patents
Automatic chain-type solid-state fermentation reaction device and method of automatic solid-state fermentation thereof Download PDFInfo
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Abstract
The invention discloses an automatic chain-type solid-state fermentation reaction device, which comprises a inoculation barrel, a spiral conveyer and a fermentation device which are connected in sequence, wherein the fermentation device comprises at least two fermentation beds which are arranged sequentially and can automatically convey and output material. The position of the input end of the fermentation bed behind can just receive the falling material from the output end of the fermentation bed before. Each section from the preparation of liquid seeds to the solid fermentation, as well as to the processing procedure after the fermentation, the mechanical automation operation is realized, labor intensity is considerably reduced, the mistakes caused by manual operation in the course of fermentation are eliminated, and the disadvantage that the quality of the fermentation product is not easy to keep table is overcome.
Description
Technical field
The present invention relates to the fermentation industry technical field, be specifically related to the method for a kind of automatic chain-type solid-state fermentation reaction device and automatic solid-state fermentation thereof.
Background technology
Solid state fermentation is a kind of fermenting process under the condition that does not have or almost do not have free-water.China just begins to use its technology in ancient times and makes food, like steamed bun etc.Owing to compare with liquid fermenting, it is low that solid fermentation has power consumption, pollutes little advantage, therefore more and more about the research of solid state fermentation in recent years.But the development of solid state fermentation is subject to its fermentation technique, and more being subject to does not have the outstanding reaction unit that is fit to its fermenting characteristic as liquid fermenting.The small-scale production of traditional solid fermentation reaction device still can be dealt with, in case but large-scale production, it can not continuous production, labour intensity is big, fermentation is wayward, be subject to the human factor influence, the unsettled shortcoming of fermentation will expose undoubtedly.
The traditional solid fermentation belongs to anaerobically fermenting or heap fermentation basically; The ulking thickness of fermentation materials is very big during heap fermentation, and the temperature contrast inside and outside the fermented substrate is big, the fermentation level significant difference; Toward fermentation level is very high inside; Outer fermentation level is then much lower, for producing fermented feed, causes the homogeneity of product of gained poor.Its major cause is that device can not be accomplished automatic stirring, walks material automatically.
To above-mentioned shortcoming, application number is to disclose a kind of automatic closed solid fermentation reactor in 95110567.1 the one Chinese patent application, is made up of main fermentation tank, ring support and material input-output system three parts; Main fermentation tank is supported on the track of ring support by 24 support rollers, can left-right rotation, and fermentation bed and tank body fuse; Adopt the bottom centre position air intake; The top air-out has realized totally-enclosed fermentation, stirring strickling automatically, self-emptying; But this complex structure, difficulty of processing is big.
Summary of the invention
The invention provides a kind of be applicable to aerobic solid-state fermentation, simple in structure, automatically stirring, walk the automatic chain-type solid-state fermentation reaction device of material automatically; Solved that present solid state fermentation complex operation, labor capacity are big, the problem of fermentation level consistence difference, helped the progress of solid-state fermentation technology.
The present invention also provides a kind of method of automatic solid-state fermentation, can reduce labour intensity, improves the quality of leavened prod.
A kind of automatic chain-type solid-state fermentation reaction device; Comprise the inoculation bucket, spiral conveyer and the fermentation unit that connect successively; Wherein said fermentation unit be at least two of arranging successively can transmit automatically, the fermentation bed of outputting material, wherein after the position of input terminus of fermentation bed just can accept the material that falls from fermentation bed output terminal formerly.
Described fermentation bed is made up of the driving mechanism of the travelling belt that is used to transmit material and this travelling belt, and wherein driving mechanism can be selected the variable-frequency motor that this area is used always for use, to control the movement velocity of travelling belt better.
Described spiral conveyer has opening for feed and stone mouth; Wherein opening for feed is used to receive and treats fermentation materials and from the bacterial classification or the seed liquor of inoculation bucket; The stone mouth is positioned at the top of fermentation bed, and the movement velocity of controlling spiral conveyer and fermentation bed with variable-frequency motor is with control load and layer thickness.
Consider from saving cost, assurance ferment effect two aspects; Described fermentation unit is preferably first fermentation bed, second fermentation bed, the 3rd fermentation bed and the 4th fermentation bed of arranging successively; Wherein the input terminus of first fermentation bed is used to accept the material that the stone mouth falls; The input terminus of second fermentation bed is used to accept the material that the output terminal of first fermentation bed falls; The input terminus of the 3rd fermentation bed is used to accept the material that the output terminal of second fermentation bed falls, and the input terminus of the 4th fermentation bed is used to accept the material that the output terminal of the 3rd fermentation bed falls.
Described inoculation bucket bottom is provided with the outlet pipe that is positioned at spiral conveyer opening for feed top; When after the slaking treat that fermentation materials gets into spiral conveyer the time; To inoculate bacterial classification or seed liquor in the bucket through outlet pipe and be sprayed onto treating on the fermentation materials after the slaking; After spiral conveyer mixes, deliver to the stone mouth and carry out stone; Also can deliver into spiral conveyer through the treat fermentation materials of track type transfer roller after with slaking, the outlet pipe of inoculation bucket bottom is arranged on track type conveyer top, bacterial classification in material conveying process, will inoculate barrel or seed liquor are sprayed onto treating on the fermentation materials after the slaking; Outlet pipe is provided with under meter and valve, so that pass through the content of moisture under meter control inoculum size and the raw material.
Described inoculation bucket top is connected with the fermentor tank of band oxygen increasing pump, so that in fermentor tank, carry out culture of strains, directly is transported to the inoculation bucket through oxygen increasing pump after the preparation seed liquor, and is time saving and energy saving.
The output terminal of described fermentation unit is connected with take-off equipment, can select the track type take-off equipment for use, like track type handling machinery etc.
As preferably; First fermentation bed, second fermentation bed, the 3rd fermentation bed and the 4th fermentation bed are positioned at same plane and along continuous straight runs staggers mutually with better transmission material; In the adjacent fermentation bed formerly the lower surface of fermentation bed and the distance between the upper surface of secondary fermentation bed be 25～35cm, preferred 30cm.There is enough oxygen to supply with the space with material after guaranteeing stone.
A kind of method of utilizing automatic chain-type solid-state fermentation reaction device to carry out automatic solid-state fermentation may further comprise the steps:
The seed liquor for preparing is sprayed onto sterilization treating on the fermentation materials after the slaking; Mix back output continuously; Through beginning fermentation behind the fs stone, carry out the fs stirring again, and in the fs stirring, accomplish the subordinate phase stone; Stone is continued on stirring limit, repeated several times limit, accomplishes until fermentation.
For reaching ferment effect preferably; The thickness of stone is preferably 10～15cm in the described stone process; Can control through the movement velocity of fermentation bed and the travelling speed of spiral conveyer, the movement velocity of fermentation bed and the travelling speed of spiral conveyer are all controlled through driving mechanism.
The number of times that stone is continued on described fermentation time and stirring limit, limit needs to confirm according to the concrete fermentation materials of treating, guarantees that ferment effect gets final product preferably.
During concrete operations, seed liquor is placed in the inoculation bucket; Treat that fermentation materials after the sterilization slaking, in being delivered to the process of spiral conveyer, opens the outlet pipe of inoculation bucket bottom, under action of gravity, bacterial classification or seed liquor be sprayed onto and treat to accomplish on the fermentation materials inoculation; The material of inoculation is mixed and is delivered to the stone mouth through spiral conveyer; Fall into the input terminus of fermentation bed, accomplish stone and begin fermentation through the travelling belt of fermentation bed exercise, the drive through travelling belt falls within on the travelling belt of secondary fermentation bed and accomplishes stirring again; Accomplish the stone of next stage in the time of stirring; According to the fermentation bed quantity that is provided with, repeat stirring limit, limit and continue stone, until accomplishing fermentation.
Apparatus of the present invention and method are applicable to the aerobic solid-state fermentation that this area is conventional.
In solid state fermentation was produced, described inoculation bucket was generally before use with 120 ℃ of sterilizations of steam 30 minutes.
In the inventive method, treat the fermentation materials slaking of sterilizing according to the existing solid state fermentation conditions of the product of concrete production and get final product, when producing fermented feed, treat that the condition of fermentation materials sterilization slaking is generally 120 ℃ of sterilizations 5～10 minutes.
The present invention has the following advantages:
The present invention is incorporated into the different operations of solid state fermentation in the one cover reaction unit; Use chain conveyor as puopulsion unit; With stone, stirring, walk material and discharging becomes robotization control; Adjust charging, stirring, walk the speed of material and discharging with driving mechanism such as variable-frequency motor, avoided existing solid-state fermentation installation to need hand feed, artificial stirring and manual cleaning's complex process.
Adopt in apparatus of the present invention to transmit to bring and carry and stir material; That has avoided using solid materials stirs device or whipping appts, makes the material can upset naturally under self gravitation, has avoided mycelial destruction; Improve fermented quality, and can realize the production of continuously fermenting.
Description of drawings
Fig. 1 is the structural representation of automatic chain-type solid-state fermentation reaction device of the present invention.
Embodiment
As shown in Figure 1, automatic chain-type solid-state fermentation reaction device of the present invention comprises the fermentor tank 9, inoculation bucket 11, spiral conveyer 13, fermentation unit and the track type handling machinery 6 that connect successively.
The bottom of fermentor tank 9 is communicated with through the pipeline that is provided with oxygen increasing pump 10 with the top of inoculation bucket 11.
The bottom of inoculation bucket 11 is provided with the outlet pipe that is positioned at spiral conveyer 13 opening for feeds top, and outlet pipe is provided with under meter 16 and valve 12.
Fermentation unit is first fermentation bed 1 of arranging successively, second fermentation bed 2, the 3rd fermentation bed 3 and the 4th fermentation bed 4; The input terminus of first fermentation bed 1 is used to accept the material that stone mouth 7 falls; The input terminus of second fermentation bed 2 is used to accept the material that the output terminal of first fermentation bed 1 falls; The input terminus of the 3rd fermentation bed 3 is used to accept the material that the output terminal of second fermentation bed 2 falls, and the input terminus of the 4th fermentation bed 4 is used to accept the material that the output terminal of the 3rd fermentation bed 3 falls.
First fermentation bed 1, second fermentation bed 2, the 3rd fermentation bed 3 and the 4th fermentation bed 4 is positioned at same plane and along continuous straight runs staggers mutually, formerly the lower surface of fermentation bed and the distance between the upper surface of secondary fermentation bed are 30cm in the adjacent fermentation bed.
Each fermentation bed is by the travelling belt 8 and the variable-frequency motor composition that drives travelling belt 8 that are used to transmit material.
Embodiment 1
Automatic production fermented bean dregs in automatic chain-type solid-state fermentation reaction device shown in Figure 1.
Slant strains: take by weighing potato 1200g, glucose 120g, NaCl 30g and agar 120g; Add water 6000mL; At 0.12MPa sterilization 20min; With Candida utilis, to be permitted in prosperous yeast, the candida tropicalis one or more be after bacterial classification is inoculated, cultivate 24h at 30 ℃ and promptly get slant strains.
Liquid spawn: take by weighing potato 1200g, glucose 120g and NaCl 30g, add water 6000mL,, promptly get liquid spawn with above-mentioned slant strains inoculation at 0.12MPa sterilization 20min.
Take by weighing potato 1200g, glucose 120g and NaCl 30g and place fermentor tank 9, add water 6000mL,, use above-mentioned liquid-spawn inoculation, cultivate 24h for 30 ℃ and promptly get seed liquor at 0.12MPa sterilization 20min.
Taking by weighing 10000 kilograms of dregs of beans, place the inherent 120 ℃ of sterilizations of quality-adjusting device 10 minutes, is to add entry at 1: 1 by the weight ratio of dregs of beans and water again, obtains treating fermentation materials.
Seed liquor in the fermentor tank 9 is transported to 30 minutes inoculation buckets 11 of 120 ℃ of sterilizations of steam through oxygen increasing pump 10,, seed liquor is sprayed onto treating on the track type conveyer accomplishes on the fermentation materials and inoculate through the flow of under meter 16 control seed liquor.
To inoculate material and after worm conveyor 13 mixes, deliver to stone mouth 7; Opening variable-frequency motor 5 makes the travelling belt 8 on first fermentation bed 1 move along counter clockwise direction; Control the travelling speed of the worm conveyor 13 and first fermentation bed 1 respectively through the variable-frequency motor 5 on the variable-frequency motor on the worm conveyor 13 and first fermentation bed 1; The inoculation material of stone mouth 7 is fallen on the travelling belt 8 on first fermentation bed 1, accomplish stone, bed thickness is 12cm.Open variable-frequency motor 14, variable-frequency motor 15 and variable-frequency motor 17 more successively; Speed through variable-frequency motor 5, variable-frequency motor 14, variable-frequency motor 15 and variable-frequency motor 17 control travelling belts 8; Travelling belt 8 on second fermentation bed 2 is moved along clockwise direction; Travelling belt 8 on the 3rd fermentation bed 3 is moved along counter clockwise direction; Travelling belt 8 on the 4th fermentation bed 4 is moved along clockwise direction, and the movement rate of the travelling belt on first fermentation bed 1, second fermentation bed 2, the 3rd fermentation bed 3 and the 4th fermentation bed 4 is consistent.The inoculation material falls into second fermentation bed, 2 input terminuss from first fermentation bed, 1 output terminal after 1 top fermentation for some time of first fermentation bed, carry out the stirring first time, accomplishes the stone on second fermentation bed 2 simultaneously; Then after 2 top fermentation for some time of second fermentation bed, fall into the 3rd fermentation bed 3 input terminuss, carry out the stirring second time, accomplish the stone on the 3rd fermentation bed 3 simultaneously from second fermentation bed, 2 output terminals; After 3 top fermentation for some time of the 3rd fermentation bed, fall into the 4th fermentation bed 4 input terminuss again, carry out stirring for the third time, accomplish the stone on the 4th fermentation bed 4 simultaneously from the 3rd fermentation bed 3 output terminals; Then in 4 top fermentation for some time of the 4th fermentation bed; After 24 hours, exported by the 4th fermentation bed 4 output terminals by fermentation ends in the fermentation bed top fermentation for assurance inoculation material; Be transported to oven dry workshop section through track type handling machinery 6 and dry, get product through pulverizing, measure packing.
Mensuration according to crude protein in following detection method: GB/T 6432 feeds; Coarse-fibred mensuration in GB/T 6434 feeds; The mensuration of crude fat in GB/T 6433 feeds; Reducing sugar method is measured digestive ferment; The measuring method of GB/T 6435 feed moisture; The mensuration of coarse ash in GB/T 6438 feeds; Amino acid whose mensuration in GB/T 18246 feeds (Threonine, Xie Ansuan, Methionin etc.); The mensuration O-Phenylene Diamine fluorescent method (VC) of vitamins C in the GB/T17816-1999 feed (total ascorbic acid); The mensuration HPLC of vitamin E in the GBT 17812-2008 feed; Vitamins B in the GBT14700-2002 feed
1Mensuration; The method for measuring of GB/T 13093-2006 yeast count, wherein substratum adopts the potato substratum, that is: potato 200 restrains, glucose 20 grams, sodium-chlor 5 grams add water to 1000ml, and incubation time is 24 hours.The above-mentioned fermented bean dregs that makes is carried out quality examination, detected result such as following table:
| Project | Detected result | Project | Detected result |
| Crude protein (%) | 48.5 | Methionin (%) | 3.8 |
| Coarse ash (%) | 4.5 | Methionine(Met)+Gelucystine (%) | 1.2 |
| Crude fat (%) | 5.3 | Threonine (%) | 1.7 |
| Robust fibre (%) | 4.4 | Xie Ansuan (%) | 2.3 |
| Moisture (%) | 10.2 | Digestive ferment (IU/ gram) | 1000 |
| Yeast quantity (hundred million/gram) | 30 | Vitamins B 1(mg/kg) | 265 |
| Vitamins C (mg/kg) | 365 | Vitamin E (mg/kg) | 133 |
Conclusion: adopt the fermented bean dregs of automatic chain-type solid-state fermentation reaction device of the present invention and fermentation process preparation, owing to be automatic stirring, fermentation is thorough, and the viable count of product, Methionin, crude protein etc. are all very high, and constant product quality, and the microbiological contamination rate is very low.
Automatic production fermented feed in automatic chain-type solid-state fermentation reaction device shown in Figure 1.
Slant strains: take by weighing potato 1200g, glucose 120g, NaCl 30g and agar 120g; Add water 6000mL; At 0.12MPa sterilization 20min; With Candida utilis, to be permitted in prosperous yeast, the candida tropicalis one or more be after bacterial classification is inoculated, cultivate 24h at 30 ℃ and promptly get slant strains.
Liquid spawn: take by weighing potato 1200g, glucose 120g and NaCl 30g, add water 6000mL,, promptly get liquid spawn with above-mentioned slant strains inoculation at 0.12MPa sterilization 20min.
Take by weighing potato 1200g, glucose 120g and NaCl 30g and place fermentor tank, add water 6000mL,, use above-mentioned liquid-spawn inoculation, cultivate 24h for 30 ℃ and promptly get seed liquor at 0.12MPa sterilization 20min.
Taking by weighing 6000 kilograms of material dregs of beans and 4000 kilograms in wheat bran, place the inherent 120 ℃ of sterilizations of quality-adjusting device 8 minutes, is to add entry at 1: 1 by the weight ratio of material and water again, obtains treating fermentation materials.
Seed liquor in the fermentor tank 9 is transported to 30 minutes inoculation buckets 11 of 120 ℃ of sterilizations of steam through oxygen increasing pump 10,, seed liquor is sprayed onto treating on the track type conveyer accomplishes on the fermentation materials and inoculate through the flow of under meter 16 control seed liquor.
To inoculate material and after worm conveyor 13 mixes, deliver to stone mouth 7; Opening variable-frequency motor 5 makes the travelling belt 8 on first fermentation bed 1 move along counter clockwise direction; Control the travelling speed of the worm conveyor 13 and first fermentation bed 1 respectively through the variable-frequency motor 5 on the variable-frequency motor on the worm conveyor 13 and first fermentation bed 1; The inoculation material of stone mouth 7 is fallen on the travelling belt 8 on first fermentation bed 1, accomplish stone, bed thickness is 12cm.Open variable-frequency motor 14, variable-frequency motor 15 and variable-frequency motor 17 more successively; Speed through variable-frequency motor 5, variable-frequency motor 14, variable-frequency motor 15 and variable-frequency motor 17 control travelling belts 8; Travelling belt 8 on second fermentation bed 2 is moved along clockwise direction; Travelling belt 8 on the 3rd fermentation bed 3 is moved along counter clockwise direction; Travelling belt 8 on the 4th fermentation bed 4 is moved along clockwise direction, and the movement rate of the travelling belt on first fermentation bed 1, second fermentation bed 2, the 3rd fermentation bed 3 and the 4th fermentation bed 4 is consistent.The inoculation material falls into second fermentation bed, 2 input terminuss from first fermentation bed, 1 output terminal after 1 top fermentation for some time of first fermentation bed, carry out the stirring first time, accomplishes the stone on second fermentation bed 2 simultaneously; Then after 2 top fermentation for some time of second fermentation bed, fall into the 3rd fermentation bed 3 input terminuss, carry out the stirring second time, accomplish the stone on the 3rd fermentation bed 3 simultaneously from second fermentation bed, 2 output terminals; After 3 top fermentation for some time of the 3rd fermentation bed, fall into the 4th fermentation bed 4 input terminuss again, carry out stirring for the third time, accomplish the stone on the 4th fermentation bed 4 simultaneously from the 3rd fermentation bed 3 output terminals; Then in 4 top fermentation for some time of the 4th fermentation bed; After 24 hours, exported by the 4th fermentation bed 4 output terminals by fermentation ends in the fermentation bed top fermentation for assurance inoculation material; Be transported to oven dry workshop section through track type handling machinery 6 and dry, get product through pulverizing, measure packing.
Detection method is with embodiment 1, and the fermented feed that embodiment 2 is made carries out quality examination, detected result such as following table:
| Project | Detected result | Project | Detected result |
| Crude protein (%) | 30.5 | Coarse ash (%) | 4.5 |
| Crude fat (%) | 4.2 | Robust fibre (%) | 5.1 |
| Moisture (%) | 10.2 | Digestive ferment (IU/ gram) | 1000 |
| Yeast quantity (hundred million/gram) | 50 | Vitamins B 1(mg/kg) | 265 |
| Vitamins C (mg/kg) | 365 | Vitamin E (mg/kg) | 133 |
Conclusion: adopt the mother of automatic chain-type solid-state fermentation reaction device of the present invention and fermentation process preparation happy strong, owing to be automatic stirring, ferment thoroughly, the viable count of product, Methionin, crude protein etc. are all very high, and constant product quality, and the microbiological contamination rate is very low.
Claims (4)
1. automatic chain-type solid-state fermentation reaction device; It is characterized in that: comprise the inoculation bucket, spiral conveyer and the fermentation unit that connect successively; Wherein said fermentation unit be for four of arranging successively can transmit automatically, the fermentation bed of outputting material, wherein after the position of input terminus of fermentation bed just can accept the material that falls from fermentation bed output terminal formerly;
Described fermentation bed is made up of the driving mechanism of the travelling belt that is used to transmit material and this travelling belt;
Described driving mechanism is a variable-frequency motor;
Described spiral conveyer has opening for feed and stone mouth, and wherein opening for feed is used to receive and treats fermentation materials and from the bacterial classification or the seed liquor of inoculation bucket;
Described fermentation unit is first fermentation bed, second fermentation bed, the 3rd fermentation bed and the 4th fermentation bed of arranging successively; Wherein the input terminus of first fermentation bed is used to accept the material that the stone mouth falls; The input terminus of second fermentation bed is used to accept the material that the output terminal of first fermentation bed falls; The input terminus of the 3rd fermentation bed is used to accept the material that the output terminal of second fermentation bed falls, and the input terminus of the 4th fermentation bed is used to accept the material that the output terminal of the 3rd fermentation bed falls;
First fermentation bed, second fermentation bed, the 3rd fermentation bed and the 4th fermentation bed are positioned at same plane and along continuous straight runs staggers mutually, and formerly the lower surface of fermentation bed and the distance between the upper surface of secondary fermentation bed are 25～35cm in the adjacent fermentation bed;
Described inoculation bucket bottom is provided with the outlet pipe that is positioned at spiral conveyer opening for feed top, and outlet pipe is provided with under meter and valve;
Described inoculation bucket top is connected with the fermentor tank of band oxygen increasing pump;
The output terminal of described fermentation unit is connected with the track type take-off equipment.
2. automatic chain-type solid-state fermentation reaction device as claimed in claim 1 is characterized in that: formerly the lower surface of fermentation bed and the distance between the upper surface of secondary fermentation bed are 30cm in the adjacent fermentation bed.
3. method of utilizing the described automatic chain-type solid-state fermentation reaction device of claim 1 to carry out automatic solid-state fermentation may further comprise the steps:
The seed liquor for preparing is sprayed onto sterilization treating on the fermentation materials after the slaking; Mix back output continuously; Through beginning fermentation behind the fs stone, carry out the fs stirring again, and in the fs stirring, accomplish the subordinate phase stone; Stone is continued on stirring limit, repeated several times limit, accomplishes until fermentation.
4. method as claimed in claim 3 is characterized in that: the thickness of stone is 10～15cm in the described stone process.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102771881A (en) * | 2012-08-10 | 2012-11-14 | 四川成都仙厨味业有限公司 | Continuous fermentation case |
| EP3395189A4 (en) * | 2015-12-24 | 2019-09-04 | CJ Cheiljedang Corporation | Continuous fermentation device |
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| CN102304557B (en) * | 2011-08-25 | 2013-06-05 | 中国农业科学院油料作物研究所 | Cascade combination type solid state fermentation method and system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771881A (en) * | 2012-08-10 | 2012-11-14 | 四川成都仙厨味业有限公司 | Continuous fermentation case |
| EP3395189A4 (en) * | 2015-12-24 | 2019-09-04 | CJ Cheiljedang Corporation | Continuous fermentation device |
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