CN101716347A - Ruminant rumen-protected lysine and production method thereof - Google Patents
Ruminant rumen-protected lysine and production method thereof Download PDFInfo
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- CN101716347A CN101716347A CN200910237384A CN200910237384A CN101716347A CN 101716347 A CN101716347 A CN 101716347A CN 200910237384 A CN200910237384 A CN 200910237384A CN 200910237384 A CN200910237384 A CN 200910237384A CN 101716347 A CN101716347 A CN 101716347A
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Abstract
The invention discloses ruminant rumen-protected lysine, which belongs to the technical field of animal nutrition. The rumen-protected lysine consists of a core and a shell, wherein the shell is rumen fat powder; and the core is a mixture of lysine serving as an active ingredient and accessories. The ruminant rumen-protected lysine comprises the following raw materials in percentages by mass: the lysine 32-48, the accessories 8-12 and the rumen fat powder 40 to 60. The invention also provides the production method of the ruminant rumen-protected lysine, which comprises the steps of pelletizing and coating. The ruminant rumen-protected lysine has a small particle size which averagely is 1.0 millimeter and consistent with a variation coefficient of 10 percent, has good liquidity and is convenient to use directly in production. The production process is simple and suitable for industrial production and the production cost is low.
Description
Technical field
The invention belongs to the Animal nutrition field, be specifically related to a kind of ruminant rumen-protected lysine and production method thereof.
Background technology
Lysine is essential amino acids and second (or the first, the difference according to the daily ration type) limiting amino acid of cow producing milk.Lysine content is generally lower in the general conventional milk cow forage.Amino acid imbalance in the dairy cow diet, the lysine wretched insufficiency causes the feed protein utilization rate low, and content of milk protein descends.Directly add common lysine in dairy cow diet, because the effect of rumen microorganism, most lysines of interpolation are degraded in cud, can not arrive small intestinal.So not only do not reach the effect of adding lysine, also can cause the waste of lysine.Unique way of dealing with problems is that the lysine that is added is carried out rumen-protected processing, and it is not degraded in cud, directly arrives small intestinal and is absorbed and used.In dairy cow diet, add the lysine of handling through overprotection (crossing cud lysine) and can make aminoacid balance more in the small intestinal chyme; thereby improve the utilization rate of dietary protein; save protein feeds; reduce the milch cow production cost; improve the content of milk protein of milk production of cow and milk; and then improve the economic benefit that milch cow produces, and reduce the discharging of nitrogen in the fecaluria simultaneously, have important function to reducing environmental pollution.
At present, the rumen-protected processing of lysine mainly contains chemoproection and two kinds of methods of physical protection.
The major product that adopts the chemoproection method to produce is the lysine metallo-chelate.The lysine metallo-chelate is to make the reaction of lysine and some metal ion generation sequestration, with lysine and metal ion sequestration together, reduces the degraded of lysine in cud with this.But because the chemical bond that lysine combines with metal ion in the lysine metallo-chelate is very insecure, it is undesirable therefore to cross the cud effect, and unstable.In addition, because animal is higher than requirement to metallic element far away to the requirement of lysine, if therefore adopt this method to replenish lysine, when lysine is satisfied the demand, must causes the seriously excessive of metallic element, even metal poisoning takes place to ruminant.
The physical protection of lysine mainly be adopt do not degrade at ruminant tumor gastric, to be at abomasum and the digestible material of small intestinal that packaging material wraps lysine processed; thereby make lysine can pass through cud as core; and in abomasum and small intestinal, discharge, digested and assimilated.The material that can be used as packaging material has dystectic fat (acid), fatty acid calcium and high molecular polymer etc.At present, wrap processed difficult point and be that the water solublity of lysine is stronger, very easily hydrolysis requires bag can not exposed at all, otherwise will have a strong impact on the cud effect fully.Have not yet to see bag by successful report,, say nothing of suitability for industrialized production even under laboratory condition, also can't realize.Wang Jianhua (Wang Jianhua; academic dissertation; 2004) and Guo Yuqin (Guo Yuqin; academic dissertation; 2006) it is processed to adopt different capsulating materials that lysine is wrapped under laboratory condition, measures bag by the cud degradation rate of product, and the result shows; 24 hours the cud degradation rate of rumen-protected lysine that adopts the Nylon Bag method to measure is respectively 79.29% and 66.62%, does not reach rumen-protected requirement.
Summary of the invention
The object of the present invention is to provide a kind of ruminant rumen-protected lysine.
Another object of the present invention is to provide the production method of above-mentioned rumen-protected lysine.
A kind of ruminant rumen-protected lysine is made up of nuclear and shell two parts, and described shell is the rumen-undegraded fat powder, and described nuclear is the mixture of active component lysine and adjuvant; Wherein, each raw material is respectively by mass percentage in the described rumen-protected lysine: lysinate 32-48%, adjuvant 8-12%, rumen-undegraded fat powder 40-60%.
Described rumen-undegraded fat powder is Bergafat T-300.
Described lysine active component is a lysine hydrochloride.
Described adjuvant is the mixture of starch and microcrystalline Cellulose, and wherein, the mass ratio of starch and microcrystalline Cellulose is 1: 1.
Described rumen-protected lysine is a graininess, and particle diameter is 0.90-1.10mm.
A kind of production method of ruminant rumen-protected lysine, carry out according to following operating procedure:
(1) preparation lysine particle
Lysine and adjuvant are pressed above-mentioned mass percent uniform mixing, add suitable quantity of water in the mixture that obtains, the preparation particle diameter is about the lysine particle of 0.40-0.60mm after modulating;
(2) drying of lysine particle
The lysine particle of making in the step (1) is carried out drying; Drying is carried out in fluidized granulation coating machine, and the heating-up temperature in the described drying is 85 ℃, air inducing frequency 35Hz, and rotating speed 45Hz, be 30 minutes drying time; The lysine particle that obtains after the drying is sieved, and collection cut size is more than or equal to the lysine particle of 0.40mm less than 0.60mm;
(3) the bag quilt of lysine particle
Bag is used fluidized granulation coating machine to carry out; concrete method for coating is: dried lysine particle is heated to 35 ℃; rumen-undegraded fat powder with fusing sprays to the lysine particle surface under the following conditions: 100 ℃ of compressed air temperature; peristaltic pump rotating speed 8rpm; air inducing frequency 28Hz, rotating speed 49Hz, 50 ℃ of air inducing temperature; 40 ℃ of leaving air temps finally obtain rumen-protected lysine.
Advantage of the present invention is:
(1) rumen-protected lysine of the present invention has higher cud effect excessively.
(2) used packaging material is the required important nutritional labeling of ruminant; it is effective to cross cud; small intestinal digestibility height; it is the high energy feed raw material that high yield cow must add; in the high yield cow daily ration, add rumen-protected lysine product of the present invention and can play the dual function and the effect of replenishing limiting amino acid and energy simultaneously; not only reduce cost of material, improved cost performance of product simultaneously greatly.
(3) adopt granulation and wrap by two stage production technologies, when the raising bag is by effect, greatly improved the physical aspect of product, granule little (particle mean size 1.0mm) and even (coefficient of variation 10% of granularity), good fluidity are convenient to directly use aborning.
(4) production technology of the present invention is simple, is fit to suitability for industrialized production, and production cost is low.
(5) through the animal experiment checking, product can obviously improve the nitrogen metabolism of milch cow.
The specific embodiment
Following examples further specify content of the present invention, but do not limit the scope of the invention.
Embodiment one rumen-protected lysine 1
(1) preparation of rumen-protected lysine
1. prepare lysine particle
After lysine hydrochloride 480g, starch 60g and microcrystalline Cellulose 60g mixed, add suitable quantity of water and be modulated into the state that can granulate, be prepared into the lysine particle of diameter 0.5mm with granulator, the spherical shot-blasting machine ball blast of reuse obtains lysine particle.
2. the drying of lysine particle
The 1. middle lysine particle for preparing of step is put into fluidized granulation coating machine carry out drying.The equipment heating-up temperature is 85 ℃, air inducing frequency 35Hz, rotating speed 45Hz, dry 30 minutes.Sieve with 30 orders and 40 mesh sieves respectively then, obtain under 30 mesh sieves, the dry lysine particle of the direct 0.40-0.60mm on 40 mesh sieve tops.
3. the bag quilt of lysine particle
400g rumen-undegraded fat powder Bergafat T-300 is melted under greater than 60 ℃ temperature; in fluidized granulation coating machine, adopt end pressure spray process that the lysine particle that 2. step prepares is wrapped quilt; the rumen-undegraded fat powder that is about to fusing sprays to the lysine particle surface; bag by the time technological parameter be: lysine particle is heated to 35 ℃; 100 ℃ of compressed air temperature; peristaltic pump rotating speed 8rpm; air inducing frequency 28Hz; rotating speed 49Hz; 50 ℃ of air inducing temperature; 40 ℃ of leaving air temps; the rumen-undegraded fat powder of fusing is all sprayed to the lysine particle surface; obtain the rumen-protected lysine 1 of diameter 0.90-1.10mm, the mass fraction of each raw material of this rumen-protected lysine 1 is a lysine hydrochloride 48%; adjuvant 12%; rumen-undegraded fat powder 40%.
(2) evaluation of rumen-protected lysine 1 stability in cud
In order to evaluate the stability of rumen-protected lysine 1 in cud that adopts said method to produce; with 3 He Sitan lactogenic cows that the permanence lymphoma stomach fistulization pipe is housed is experimental animal, and lysine is at the cud degradation rate of 0,2,4,8,12,24 hour 6 incubation time point in the employing cud Nylon Bag method mensuration rumen-protected lysine 1.Measurement result sees Table 1.
The degradation rate of lysine 1 in cow rumen that table 1 Nylon Bag method is measured
Measurement result shows that the lysine degradation rate that the rumen-protected lysine 1 that the present invention produces was cultivated 12 hours is 82% in cud, cultivating 24 hours degradation rates in cud is 87.93%.
Example two: rumen-protected lysine 2
(1) preparation of rumen-protected lysine
1. prepare lysine particle
After lysine hydrochloride 400g, starch 50g and microcrystalline Cellulose 50g mixed, add water and be modulated into the state that can granulate, be prepared into the lysine particle of diameter 0.5mm with granulator, the spherical shot-blasting machine ball blast of reuse obtains lysine particle.
2. the drying of lysine particle
The 1. middle lysine particle for preparing of step is put into fluidized granulation coating machine carry out drying.Equipment heating-up temperature scope is 85 ℃, air inducing frequency 35Hz, rotating speed 45Hz, dry 30 minutes.Sieve with 30 orders and 40 mesh sieves respectively then, obtain under 30 mesh sieves, the dry lysine particle of the diameter 0.40-0.60mm on 40 mesh sieve tops.
3. the bag quilt of lysine particle
500g rumen-undegraded fat powder Bergafat T-300 is melted under greater than 60 ℃ temperature; in fluidized granulation coating machine, adopt end pressure spray process that the lysine particle that 2. step prepares is wrapped quilt; the rumen-undegraded fat powder that is about to fusing sprays to the lysine particle surface; bag by the time technological parameter be: lysine particle is heated to 35 ℃; 100 ℃ of compressed air temperature; peristaltic pump rotating speed 8rpm; air inducing frequency 28Hz; rotating speed 49Hz; 50 ℃ of air inducing temperature; 40 ℃ of leaving air temps; the rumen-undegraded fat powder of fusing is all sprayed to the lysine particle surface; obtain the exsiccant rumen-protected lysine 2 of diameter 0.90-1.10mm, the mass fraction of each raw material of this rumen-protected lysine 2 is a lysine hydrochloride 40%; adjuvant 10%; rumen-undegraded fat powder 50%.
(2) evaluation of rumen-protected lysine 2 stability in cud
In order to evaluate the stability of rumen-protected property lysine 2 in cud that adopts method for preparing; with 3 He Sitan lactogenic cows that the permanence lymphoma stomach fistulization pipe is housed is experimental animal, and lysine is at the cud degradation rate of 0,2,4,8,12,24 hour 6 incubation time point in the employing cud Nylon Bag method mensuration rumen-protected lysine product 2.Measurement result sees Table 2.
The degradation rate of lysine 2 in cow rumen that table 2 Nylon Bag method is measured
Measurement result shows; the lysine degradation rate that the rumen-protected lysine 2 that the present invention produces was cultivated 12 hours in cud is 55.11%; cultivating 24 hours degradation rates in cud is 57.26%; promptly this product stops in cow rumen had 44.89% lysine not to be degraded by cud in 12 hours; stopping in cow rumen had 42.74% lysine not to be degraded by cud in 24 hours; illustrate that this product had preferably the cud effect, more stable in cud.
The lysine cud degradation rate of above-mentioned result of the test explanation rumen-protected lysine product of the present invention descends with the raising of fatty powder ratio, and promptly the fatty powder ratio is high more, and it is good more by effect to wrap, otherwise then poor more.If continue to increase the ratio of fatty powder, its protection effect can be better.But along with the increase of fatty powder consumption, the content of the effective ingredient in the product (lysine) can reduce gradually.In order to guarantee content of effective and to cross the cud effect and take into account simultaneously, the consumption of each raw material in the rumen-protected property lysine will be limited within the scope of the invention.
Example three rumen-protected lysines are to the evaluation of lactogenic cow biological effectiveness
In order to evaluate the biological effectiveness of the rumen-protected lysine product that adopts said method production; with 3 He Sitan lactogenic cows that the permanence lymphoma stomach fistulization pipe is installed is experimental animal; adopt 3 * 3 latin square experiments design to carry out metabolic test; measuring rumen-protected lysine 2 products (60 gram/heads day) that add the embodiment of the invention 2 methods in the daily ration and produce to the influence of milch cow digestion and metabolism and other main indexs of correlation, organizes in contrast not add the rumen-protected lysine product.Test mainly the results are shown in Table 3.
The influence of 2 pairs of milch cow digestion and metabolism of table 3 rumen-protected lysine and other main indexs of correlation
By table 3 as seen; in lactogenic cow daily ration, add rumen-protected lysine of the present invention; can improve total amino acids and lysine content, blood plasma total protein content, plasma free total amino acids and lysine content in the utilization rate, milk of digestibility, feed nitrogen of daily ration fibrous matter, plasma glucose content to a certain extent, and reduce urea nitrogen content in the blood plasma.The The above results explanation; in lactogenic cow daily ration, add rumen-protected lysine product of the present invention; can improve nitrogen metabolism and the energy metabolism of lactogenic cow greatly, improve the utilization rate of dietary protein, these effects are very beneficial for the give milk raising of performance and milk quality of lactogenic cow.
List of references
1, Wang Jianhua, the development of ruminally-protected acidic amino acid and the research that the Inner Mongolia White Cashmere Goat digestion and metabolism is influenced thereof, academic dissertation, 2004.
2, Guo Yuqin, the rumen-protected and effect assessment research of methionine and lysine, academic dissertation, 2006.
Claims (6)
1. a ruminant rumen-protected lysine is made up of nuclear and shell two parts, it is characterized in that described shell is the rumen-undegraded fat powder, and described nuclear is the mixture of active component lysine and adjuvant; Wherein, each raw material is respectively by mass percentage in the described rumen-protected lysine: lysine 32-48%, adjuvant 8-12%, rumen-undegraded fat powder 40-60%.
2. ruminant rumen-protected lysine according to claim 1 is characterized in that, described rumen-undegraded fat powder is Bergafat T-300.
3. ruminant rumen-protected lysine according to claim 1 is characterized in that, described lysine active component is a lysine hydrochloride.
4. ruminant rumen-protected lysine according to claim 1 is characterized in that, described adjuvant is the mixture of starch and microcrystalline Cellulose, and wherein, the mass ratio of starch and microcrystalline Cellulose is 1: 1.
5. according to the arbitrary described ruminant rumen-protected lysine of claim 1-4, it is characterized in that described rumen-protected lysine is a graininess, particle diameter is 0.90-1.10mm.
6. the production method of a ruminant rumen-protected lysine is characterized in that, may further comprise the steps:
(1) preparation lysine particle
Lysine and adjuvant are pressed above-mentioned mass percent uniform mixing, add suitable quantity of water in the mixture that obtains, the preparation particle diameter is the lysine particle of 0.40-0.60mm after modulating;
(2) drying of lysine particle
The lysine particle of making in the step (1) is carried out drying; Dry carry out in fluidized granulation coating machine, described heating-up temperature when dry is 85 ℃, air inducing frequency 35Hz, and rotating speed 45Hz, be 30 minutes drying time; The lysine particle that obtains after the drying is sieved, and collection cut size is more than or equal to the lysine particle of 0.40mm less than 0.60mm;
(3) the bag quilt of lysine particle
The dry lysine particle that step (2) is obtained adopts end pressure spray process to wrap quilt in fluidized granulation coating machine; concrete method for coating is: dried lysine particle is heated to 35 ℃; rumen-undegraded fat powder with fusing sprays to the lysine particle surface under the following conditions: 100 ℃ of compressed air temperature; peristaltic pump rotating speed 8rpm; air inducing frequency 28Hz, rotating speed 49Hz, 50 ℃ of air inducing temperature; 40 ℃ of leaving air temps finally obtain rumen-protected lysine.
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Cited By (13)
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| WO2012027876A1 (en) * | 2010-08-28 | 2012-03-08 | 青岛农业大学 | Rumen bypass amino acid high-energy composition for cow |
| CN103689239A (en) * | 2013-12-23 | 2014-04-02 | 宜兴市天石饲料有限公司 | Preparation method of rumen-through double-layer coated betaine |
| CN103918876A (en) * | 2014-04-16 | 2014-07-16 | 陈林书 | Method for producing rumen-passing protected product for ruminants and granules of rumen-passing protected product |
| CN104431369A (en) * | 2014-12-17 | 2015-03-25 | 宁夏伊品生物科技股份有限公司 | Rumen-protected lysine feed |
| CN104472877A (en) * | 2014-12-17 | 2015-04-01 | 宁夏伊品生物科技股份有限公司 | Preparation process of L-lysine product |
| CN104587482A (en) * | 2015-01-14 | 2015-05-06 | 长春大合生物技术开发有限公司 | Coating material used for rumen by-pass amino acid preparation and rumen by-pass amino acid preparation |
| CN104970228A (en) * | 2015-06-16 | 2015-10-14 | 浙江万方生物科技有限公司 | Preparation method for rumen-protected lysine |
| CN105076828A (en) * | 2015-08-28 | 2015-11-25 | 新疆西部牧业股份有限公司 | Cow-dedicated compound premix for balancing cotton source and corn source amino acid balance |
| CN105076729A (en) * | 2015-09-16 | 2015-11-25 | 中粮饲料有限公司 | Feed additive microcapsule and preparation method thereof |
| CN107115315A (en) * | 2017-05-09 | 2017-09-01 | 北京东方天合生物技术有限责任公司 | Formula and preparation method for the rumen-protected nicotinic acid that prevents ketosis of dairy cows |
| CN108029873A (en) * | 2017-12-28 | 2018-05-15 | 长沙善道新材料科技有限公司 | A kind of feed additive for ruminant |
| CN109414039A (en) * | 2016-08-10 | 2019-03-01 | 生物科学株式会社 | The manufacturing method of preparation for rumen and thus obtained granule |
| CN113115867A (en) * | 2021-05-18 | 2021-07-16 | 河南东灿生物科技有限公司 | Rumen-bypass feed additive special for beef cattle and sheep in later fattening period and preparation method and application thereof |
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| WO2012027876A1 (en) * | 2010-08-28 | 2012-03-08 | 青岛农业大学 | Rumen bypass amino acid high-energy composition for cow |
| CN103689239A (en) * | 2013-12-23 | 2014-04-02 | 宜兴市天石饲料有限公司 | Preparation method of rumen-through double-layer coated betaine |
| CN103918876A (en) * | 2014-04-16 | 2014-07-16 | 陈林书 | Method for producing rumen-passing protected product for ruminants and granules of rumen-passing protected product |
| CN104431369B (en) * | 2014-12-17 | 2017-05-24 | 宁夏伊品生物科技股份有限公司 | Rumen-protected lysine feed |
| CN104472877A (en) * | 2014-12-17 | 2015-04-01 | 宁夏伊品生物科技股份有限公司 | Preparation process of L-lysine product |
| CN104431369A (en) * | 2014-12-17 | 2015-03-25 | 宁夏伊品生物科技股份有限公司 | Rumen-protected lysine feed |
| CN104472877B (en) * | 2014-12-17 | 2017-07-18 | 宁夏伊品生物科技股份有限公司 | The preparation technology of L lysine products |
| CN104587482A (en) * | 2015-01-14 | 2015-05-06 | 长春大合生物技术开发有限公司 | Coating material used for rumen by-pass amino acid preparation and rumen by-pass amino acid preparation |
| CN104970228A (en) * | 2015-06-16 | 2015-10-14 | 浙江万方生物科技有限公司 | Preparation method for rumen-protected lysine |
| CN105076828A (en) * | 2015-08-28 | 2015-11-25 | 新疆西部牧业股份有限公司 | Cow-dedicated compound premix for balancing cotton source and corn source amino acid balance |
| CN105076729A (en) * | 2015-09-16 | 2015-11-25 | 中粮饲料有限公司 | Feed additive microcapsule and preparation method thereof |
| CN109414039A (en) * | 2016-08-10 | 2019-03-01 | 生物科学株式会社 | The manufacturing method of preparation for rumen and thus obtained granule |
| CN107115315A (en) * | 2017-05-09 | 2017-09-01 | 北京东方天合生物技术有限责任公司 | Formula and preparation method for the rumen-protected nicotinic acid that prevents ketosis of dairy cows |
| CN108029873A (en) * | 2017-12-28 | 2018-05-15 | 长沙善道新材料科技有限公司 | A kind of feed additive for ruminant |
| CN113115867A (en) * | 2021-05-18 | 2021-07-16 | 河南东灿生物科技有限公司 | Rumen-bypass feed additive special for beef cattle and sheep in later fattening period and preparation method and application thereof |
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