CN101715665B - Method for improving germination rate of monkshood seed - Google Patents
Method for improving germination rate of monkshood seed Download PDFInfo
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- CN101715665B CN101715665B CN2009103126956A CN200910312695A CN101715665B CN 101715665 B CN101715665 B CN 101715665B CN 2009103126956 A CN2009103126956 A CN 2009103126956A CN 200910312695 A CN200910312695 A CN 200910312695A CN 101715665 B CN101715665 B CN 101715665B
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Abstract
The invention discloses a method for improving a germination rate of a monkshood seed. Before the monkshood seed is sown, the monkshood seed is soaked in a GA3 solution with the concentration of 400-1,000mg/L. By soaking the monkshood seed by gibberellin with proper concentration, the method can obviously improve the germination rate and the germinability of the monkshood seed and increase the fresh weight of the overground part of monkshood seedlings, and the seedlings are rapid to emerge and are in order.
Description
Technical field
The invention belongs to medicinal material culture technique field, particularly a kind of method that improves germination rate of monkshood seed.
Background technology
The rhizome of Chinese monkshood (Aconitum carmichaeli Debx.) is Ranunculaceae (Ranunculaceae) herbaceos perennial; Its sub-root is medicinal material river, birth canal ground, a famous traditional river monkshood; Have pharmacological action widely and clinical application, distribute extensively in China, artificial cultivation is with a long history.
At present; Because of cultivation condition and geographical position limitting, monkshood production all is the vegetative propagation pattern that adopts numerous kind in high mountain, Pingba regional culture over the years, and cultivation is high with kind of cost; And output reduces in recent years, disease is serious, quality descends, and the monkshood area under cultivation reduces year by year.Seminal propagation has unit are and produces advantages such as high, with low cost, the fast natural disposition of seedling amount is good; The one strain rhizome of Chinese monkshood becomes strain can breed 500 seeds every year at least; Therefore through seminal propagation; And then expanded the piece root so that seedling produced and produce with kind producing a monkshood as the land for growing field crops, can change traditional monkshood production model, become present stage and break the new approaches of monkshood industry development bottleneck.
But because rhizome of Chinese monkshood seed has hibernation feature, its resting stage is long, germination is slow, germination rate is low, germination is irregular; Rhizome of Chinese monkshood seed germination rate directly has influence on the success rate of monkshood breeding and culturing.Therefore, how to improve rhizome of Chinese monkshood seed germination rate and become the key technique of monkshood cultivation mode for a change.
Li Henan is in " radix coniti coreani seed germination and water-soluble endogenous inhibiting substances research " (Anhui agricultural science .2008,36 (27): 11790-11792) disclose following variable concentrations gibberellin (CA in the literary composition
3) soak the influence to rhizome of Chinese monkshood seed germination rate in 24 hours: 0,50,100,150,200,250,300,350ug/ml (being mg/L); Its conclusion is: the rhizome of Chinese monkshood seed that 200-300ug/ml (being mg/L) gibberellin soaked 24 hours has higher germination rate and germination vigor; Rhizome of Chinese monkshood seed germination rate and germination vigor reduce on the contrary during to 350ug/ml (being mg/L).
Li Henan, Zhou Meng is in " the different chemical medicament is to the influence of radix coniti coreani seed germination " (the journal .2008 of Jilin agricultural science and technology institute, 17 (4): 5-6) disclose following variable concentrations gibberellin (CA in the literary composition
3) soak the influence to rhizome of Chinese monkshood seed germination rate in 12 hours: 100,200,300mg/ml (promptly be respectively 0.1,0.2,0.3mg/L), and the potassium permanganate of variable concentrations soaks the influence to rhizome of Chinese monkshood seed germination rate in 12 hours.Be superior to the effect that potassium permanganate is handled with the effect of gibberellin treatment, and with 200mg/ml (being 0.2mg/L) be superior to 100 with the effect of 300mg/ml (promptly be respectively 0.1 and 0.3mg/L) processing; The concentration of gibberellin is all very little in this method.
From the disclosed content of above-mentioned prior art, obviously, even with a kind of medicament (like gibberellin), rhizome of Chinese monkshood seed is soaked in same being used to, and its result of study that influences to germination rate of monkshood seed also is uncertain.In " radix coniti coreani seed germination and water-soluble endogenous inhibiting substances research " literary composition, soak 24 hours better with 200-300ug/ml (being mg/L) gibberellin, be lower than 200mg/L be higher than 300ug/ml downward trend all arranged; And in " the different chemical medicament is to the influence of radix coniti coreani seed germination " literary composition, then soak 12 hours better with 200mg/ml (being 0.2mg/L) gibberellin, be below or above all decreasing of this concentration.The concentration of these two the bests (scope) differs bigger, thereby can not represent variable concentrations gibberellin to be used to soak the general trend of rhizome of Chinese monkshood seed to the influence of its germination rate; Can't judge also that from its disclosed content the gibberellin of other concentration is used to soak rhizome of Chinese monkshood seed and will produces what kind of influence to its germination rate.
Summary of the invention
Main purpose of the present invention is to be directed against problems such as the seed sprouting that exists in the above-mentioned rhizome of Chinese monkshood seeds cultivation is slow, germination rate is low, germination is irregular; A kind of new method that improves germination rate of monkshood seed is provided; Improving rhizome of Chinese monkshood seed germination rate and germination vigor, and make rhizome of Chinese monkshood seed sprouting more rapidly, more neat.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of method that improves germination rate of monkshood seed before with rhizome of Chinese monkshood planting seed, is the GA of 400mg/L-1000mg/L in concentration with rhizome of Chinese monkshood seed earlier
3Immersion treatment in (gibberellin) solution.
GA
3The concentration of (gibberellin) solution is good with 600mg/L.
Soaking temperature can be 10-40 ℃, is good with 15-30 ℃.
Soak time can be 8-48 hour, is good with 18-30 hour.
When adopting gibberellin that rhizome of Chinese monkshood seed is handled, the concentration of gibberellin is most important, directly has influence on the germination vigor and the germination rate of rhizome of Chinese monkshood seed.
Owing to from prior art, can't judge the influence trend of gibberellin variable concentrations to germination rate of monkshood seed; The inventor is through a large amount of experimental studies and data comparative analysis; The unexpected discovery when gibberellin concentration is 400mg/L-1000mg/L, can make rhizome of Chinese monkshood seed germination rate and germination vigor be improved significantly; And rhizome of Chinese monkshood seedling acrial part fresh weight is increased, it is comparatively rapid, neat to emerge.Disclosed 200-300ug/ml (being mg/L) has notable difference in this concentration range and " radix coniti coreani seed germination and the water-soluble endogenous inhibiting substances research ", and is more remarkable with disclosed 0.2mg/L difference in " the different chemical medicament is to the influence of radix coniti coreani seed germination ".
Compared with prior art, the invention has the beneficial effects as follows:
The inventive method is through adopting gibberellin (400mg/L-1000mg/L) the immersion treatment rhizome of Chinese monkshood seed of debita spissitudo; Rhizome of Chinese monkshood seed germination rate and germination vigor are improved significantly; And rhizome of Chinese monkshood seedling acrial part fresh weight is increased, it is comparatively rapid, neat to emerge.
Description of drawings
Fig. 1 is GA
3Influence to germination rate of monkshood seed.
Embodiment
Below in conjunction with embodiment the present invention is made further detailed description.
But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment.
Embodiment
Present embodiment is investigated the influence of the gibberellin of variable concentrations to germination rate of monkshood seed and germination vigor etc. for adopting the gibberellin immersion treatment rhizome of Chinese monkshood seed of variable concentrations:
One, test material:
1. rhizome of Chinese monkshood seed: supply the examination rhizome of Chinese monkshood (Aconitum carmichaeli Debx.) seed to gather from the Qingchuan County, Sichuan in by the end of October, 2008, identify by professor Wu Wei of university of Sichuan Agricultural University in.Be stored in the drier subsequent use behind the rhizome of Chinese monkshood seed natural air drying of gathering.Test is cleaned seed before handling, and chooses full seed, and nothing is gone mouldy, the seed of no damage by disease and insect.
2. instrument and equipment: three circle boards, 0～150mm slide measure, ten thousand/electronic balance (BP110S), PGX type multi-segment Programmable illumination box, 2cm * 12cm standard germination box, spad-502 chlorophyll measuring appearance, ruler.
Two, test method
7 processing (i.e. 7 groups) are established in test, and each is handled and sees table 1 (A
1Be control group, A
2-A
7Be variable concentrations GA
3The solution-treated group), 4 repetitions, every germination box is 1 repetition, each repeats 100 rhizome of Chinese monkshood seeds.Earlier with the GA of rhizome of Chinese monkshood seed at variable concentrations
3Evenly disseminate on the husky bed of having sterilized after soaking 24h (20 ℃) in the solution, and cover husky 1～2mm.After the germination box is placed illumination box, temperature is controlled at 15～20 ℃ (12h illumination/dark) and carries out germination test.Regularly observe, write down the germinative number of rhizome of Chinese monkshood seed in every box every afternoon, up to the germinative number of every box compare with the previous day all do not have marked change till, calculate germination vigor, the germination rate of seed.Wherein, germination vigor and germination rate are represented with the mean value that repeats several times.During off-test; From each germination box, randomly drawing 10 strain rhizome of Chinese monkshood seedling investigates; Selected seedling is measured petiole length, blade length and width with slide measure; With the fresh weight of ten thousand/electronic balance weighing acrial part, measure chlorophyll content with spad-502 chlorophyll measuring appearance, and calculate blade aspect ratio, leaf area.11d calculates germination vigor in the process of the test, and 15d calculates germination rate, and measures other index on the same day.After the survey data were put in order, utilization DPS Version 7.05 carried out correlation analysis.
7 processing of table 1 test
Three, result of the test and analysis
The different disposal group is seen below table 2 to the result that influences of the germination vigor of rhizome of Chinese monkshood seed, germination rate etc.
Table 2GA
3Rhizome of Chinese monkshood seed germination and the growth of seedling amount handled
| Handle | Germination vigor (%) | Germination rate (%) | Fresh weight (mg/ strain) | Ratio of length to breadth | Leaf area (mm 2) | Petiole long (mm) | Chlorophyll |
| A 1(CK) | 50.21 cC | 55.73 cC | 38.10 aA | 1.52 aA | 50.88 aA | 19.01 cB | 35.21 abAB |
| A 2 | 65.71 bB | 72.00 bB | 38.65 aA | 1.44 bB | 49.12 aA | 19.08 cB | 37.63 aA |
| A 3 | 69.87 bAB | 77.55 abAB | 36.92 aA | 1.49 abAB | 49.36 aA | 19.18 cB | 36.86 aAB |
| A 4 | 74.23 abA | 82.27 aA | 39.91 aA | 1.34 cdC | 48.99 aA | 21.39 bcB | 35.83 abAB |
| A 5 | 77.04 aA | 85.44 aA | 39.84 aA | 1.33 cdC | 48.77 aA | 22.33 bB | 33.57 bcB |
| A 6 | 76.77 aA | 82.53 aA | 42.08 aA | 1.29 dC | 49.09 aA | 22.05 bB | 35.74 abAB |
| A 7 | 74.47 abA | 82.79 aA | 44.45 aA | 1.36 cC | 48.68 aA | 25.63 aA | 32.01 cB |
Annotate: letter representation is represented α=0.05 level through the result of LSD test with lowercase in the table, representes α=0.01 level with capitalization, and alphabetical different table differential is different significantly.
1. the variable concentrations gibberellin treatment is to the influence of rhizome of Chinese monkshood seed germination characteristic
By Fig. 1 (GA
3Influence to germination rate of monkshood seed) can know; Rhizome of Chinese monkshood seed germination process was long, and each gibberellin treatment group germination rate and growth rate thereof generally were higher than control group since the 5th day, was the germination rush day about the 11st day; Compare with control group, it emerges more rapid, neat.Wherein, GA
3Concentration is that the germination rate under the 600mg/L condition is totally maximum.
Visible by table 2, use variable concentrations GA
3After solution soaking was handled rhizome of Chinese monkshood seed, its germination vigor and germination rate be contrast all.Wherein: maximum with potentiality of seed under the 600mg/L concentration and germination rate, reach 77.04% and 85.44% respectively; Germination vigor and germination rate after 400～1000mg/L concentration is handled all are significantly higher than 100mg/L and 200mg/L.
2. the variable concentrations gibberellin treatment is to the influence of rhizome of Chinese monkshood seedling acrial part fresh weight
It can also be seen that from table 2, through the GA of variable concentrations
3After solution soaking was handled, the performance of rhizome of Chinese monkshood seedling acrial part fresh weight was different.Wherein, except that 200mg/L concentration, seedling overground part fresh weight all is higher than contrast after other concentration solution-treated, and has along with GA
3The concentration of solution raises, and the overground part fresh weight has the trend that thereupon increases: 1000mg/L GA
3Fresh weight is maximum during concentration, reaches 44.45mg, and 800mg/L takes second place.
In addition, it can also be seen that, behind the variable concentrations gibberellin treatment, no significant, regular influences such as rhizome of Chinese monkshood seedling leaves aspect ratio, leaf area, petiole length, chlorophyll content in leaf blades from table 2.
Four, conclusion (of pressure testing)
Rhizome of Chinese monkshood seed is embarrassed germination seed, and it is long that it begins germinating time under the normal condition, and germination process is long, and germination vigor and germination rate are lower.And after 400mg/L-1000mg/L gibberellin immersion treatment; Its germination vigor and germination rate all are significantly higher than contrast; The time that seed begins to germinate, obviously contrast was shorter, and it is more neat more rapidly to emerge, and explained that the gibberellin of suitable concentration can be broken rhizome of Chinese monkshood seed dormancy, improved seed vitality.Result of study is further illustrated in this scope inner gibberellin concentration rising (apparently higher than the typical concentrations of prior art report) inhibitory action is not appearred in rhizome of Chinese monkshood seed germination.
Claims (3)
1. method that improves germination rate of monkshood seed is characterized in that: before with rhizome of Chinese monkshood planting seed, earlier with rhizome of Chinese monkshood seed at GA
3Immersion treatment in the solution, the temperature of immersion treatment are 10-40 ℃, and the time of immersion treatment is 8-48 hour, said GA
3Solution concentration is 600mg/L.
2. the method for raising germination rate of monkshood seed according to claim 1 is characterized in that: the temperature of described immersion treatment is 15-30 ℃.
3. the method for raising germination rate of monkshood seed according to claim 1 is characterized in that: the time of described immersion treatment is 18-30 hour.
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| CN102124889B (en) * | 2010-12-08 | 2012-07-04 | 陇西奇正药材有限责任公司 | Seedling culture method for Tibetan medicine Aconitum sinomontanum |
| CN102612894A (en) * | 2012-04-28 | 2012-08-01 | 南京林业大学 | Method for improving germination percentage of European hornbeam seeds |
| CN104541665B (en) * | 2015-01-23 | 2017-04-12 | 中国农业科学院特产研究所 | Method for breaking dormancy of aconitum kusnezoffii reichb seeds |
| CN105612856A (en) * | 2016-01-18 | 2016-06-01 | 云南农业大学 | Simple method for improving germination rate of aconitum vilmorinianum kom seeds |
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| CN1884490A (en) * | 2006-06-30 | 2006-12-27 | 李昌禹 | Agrobacterium rhizogenes mediated aconitum coreanum and its obtaining method |
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| CN1884490A (en) * | 2006-06-30 | 2006-12-27 | 李昌禹 | Agrobacterium rhizogenes mediated aconitum coreanum and its obtaining method |
Non-Patent Citations (4)
| Title |
|---|
| 周丽霞等.紫花高乌头种子萌发特性研究.《2007年中国园艺学会观赏园艺专业委员会年会论文集》.2007,448-452. * |
| 张岩等.黄花乌头种子萌发特性的研究.《中国观赏园艺研究进展(2005)》.2005,(第1版),399-402. * |
| 李赫男.黄花乌头种子萌发与水溶性内源抑制物质研究.《安徽农业科学》.2008,第36卷(第27期),11790-11792. * |
| 陈艳等.乌头种子萌发特性初探.《种子》.2009,第28卷(第6期),91-94. * |
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