CN101703005A - Microbial strain and application thereof - Google Patents
Microbial strain and application thereof Download PDFInfo
- Publication number
- CN101703005A CN101703005A CN200910218307A CN200910218307A CN101703005A CN 101703005 A CN101703005 A CN 101703005A CN 200910218307 A CN200910218307 A CN 200910218307A CN 200910218307 A CN200910218307 A CN 200910218307A CN 101703005 A CN101703005 A CN 101703005A
- Authority
- CN
- China
- Prior art keywords
- root
- knot nematode
- microbial strain
- nematode
- microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a microbial strain and application thereof, and belongs to the technical field of biological pesticide. The microbial strain of the invention is Stropharia sp. 2052, the 2052 strain is preserved in CGMCC on November 5th, 2009, and the preservation number is CGMCC NO.3399. The microbial strain is applied in preparing a preparation for preventing and controlling root-knot nematode. A bactericide prepared by the microbial strain of the invention has more than 60 percent of greenhouse prevention effect on tomato root-knot nematode, has more than 50 percent of greenhouse prevention effect on tobacco root-knot nematode, and can obviously promote the growth of plants. The preparation prepared by the invention for preventing and controlling the root-knot nematode has the advantages of high efficiency, low toxin and no residue.
Description
Technical field:
The present invention relates to a kind of microbial strains and application thereof, the microorganism belonging to genus technical field of pesticide.
Technical background:
Plant nematode is one of disease that generally takes place on the crop, endangers nearly all crop.Only the annual loss that causes in the whole world of root-knot nematode (Meloidogyne spp.) is just up to tens billion of dollars.Lack resistant variety owing to produce to go up, crops cropping index height, the use of chemical nematode killing agent is restricted, and the needs of modern agriculture sustainable development, and the biological control root-knot nematode comes into one's own day by day.
The special mushroom member of one class is arranged in the Nematophagous fungi, be also referred to as food nematode basidiomycetes.These mushrooms are because of its attack and utilize the behavior of nematode to be called as " mushroom of food meat ".Numerous species can produce some accessory structures in these mushrooms, comprising water clock shape slimeball and the stephanocyst as predacious organs, also has toxinferous toxin ball and toxoid ball, and these accessory structures are by adhering to the mode effect nematode that seizure or toxin are knocked down.In the biological control of nematode, has potential and actual using value.
Summary of the invention:
The object of the present invention is to provide a kind of microbial strains and application thereof.
The bacterial strain with suitability for industrialized production prospect is sought in the present invention's screening from food nematode basidiomycetes, and the biological prevention and control agent of a kind of tool better prevention root-knot nematode is provided for agricultural production.The present invention specifically is achieved in that
The inventor is in early-stage Study, the basidiomycetes Stropharia rugoso-annulata 2052 (Stropharia sp.2052) that screening has obtained to have good killing root-knot nematode function, 2052 bacterial strains have been stored in China Microbial Culture Preservation Commission common micro-organisms center, preserving number: CGMCC No.3399 on November 5th, 2009.
Stropharia rugoso-annulata (Stropharia) is under the jurisdiction of Basidiomycotina, Hymenomycetes, Agaricales, Stropharia rugoso-annulata section.Its main morphological feature is: the spore of puce to infuscate arranged, be common in places such as the woods, meadow, compost, animal wastes.Fruit body is medium to bigger, bacteria cover diameter 60～160 (200) mm, and flat hemispherical, hemispherical are to flat, and the wine red brown transfers reddish brown brown or cheese look gradually to, and yellow sometimes cheese look also has the claret filament to distribute therebetween.Cap when children taper is to bell, and is bell to open and flat when ripe, level and smooth or cilium shape scale is arranged, and sticking slightly when moistening, there is the velum relic at the initial stage, after polish, the edge is sharp, often inwardly curls.The lamella initial stage is dark brown slightly, and after stain deepens, and dusty blue is closely given birth to and adhesion to atropurpureus, and is close, and broad is not isometric, and the edge is slightly white.Stem white, slightly yellow, thicker sometimes, 70～150 * 15～30mm, cylindrical, base portion slightly expands, and is solid.Collarium is thicker, white.Stem is at smooth, the no squama in the following position of collarium.Bacterial context white is thicker.Green grass fragrance is arranged.The spore print puce, spore is oval to avette, heavy wall, 9.5～12 * 7～9 μ m have germ pore.Singly life or all living creatures are in habitats such as the woods and meadows.The pure culture of nearly all Stropharia fungi can both produce the accessory structure nematoblast, and this structure is considered to a key character of this genus classification of fungi
The present invention by solid culture, obtains to comprise the thalline culture of mycelia and mycelia accessory structure with Stropharia rugoso-annulata 2052, and solid culture is directly made granula, as the application of killing root-knot nematode preparation.
Stropharia rugoso-annulata (Stropharia) original strain poor growth. we are separated to different strains from soil; wherein most characteristic that does not possess we required " obvious stable control efficiency; comprehensive proterties is good; be easy to large-scale production ". after bacterial strain is screened and improves; it not only has good control efficiency to root-knot nematode (Meloidogyne spp.) to have obtained stable bacterial strain 2052., has possessed the basic proterties of suitability for industrialized production simultaneously.
Because (spontaneous, physics or chemical) sudden change, protoplast are merged, and conversion or other can change by biotechnology or be transformed, the mutant strain of any bacterial strain of this bacterial classification, mutation and derivative are all in the protection domain of this patent in view of this bacterial strain.
This biological prevention and control agent can mix use with other nematicide agricultural chemicals, plant growth regulator, but must guarantee that institute's confection has no side effect to the Stropharia rugoso-annulata that the present invention adopts.
Nematode biological prevention and control agent of the present invention is generally used when crop sowing or transplanting.Method is during when sowing and seed are manured into soil jointly, during transplanting this preparation to be applied around the crop rhizosphere.Serious area is taken place nematode can impose in process of crop growth once, and method is that this preparation is applied around the crop rhizosphere.
The present invention compares with the existing plant nematode medicine that kills, and has the advantage of efficient, low toxicity and noresidue.
Embodiment:
The fermentation method for producing of The compounds of this invention (conventional method):
1, the mycelium with 2052 bacterial strains is inoculated on the test tube culture medium slant, and culture medium prescription is the PDA medium, cultivates 4-8 days down, obtains the test tube kind for 20-30 ℃.
2, cultured solid seed is inserted in the solid culture medium, the prescription of medium is: 70-80% wheat berry, 10-20% glucose, and 5-10% calcium carbonate is cultivated after 25-35 days for 22-28 ℃, and 40-45 ℃ of temperature condition made granula after the oven dry down.
Preparation of the present invention is to the efficiency test of root-knot nematode:
1, the cultivation of Meloidogyne incognita (Meloidogyne incognita)
The tomato root that has Meloidogyne incognita of hot-house culture is cleaned with sterile water, the egg capsule of dissecting needle picking root-knot nematode places the KCl solution of 0.4M, use NaClO solution washing and the sterilization of 4ml sterile water (three times), the two anti-solution of the aseptic green grass or young crops-streptomycin of 1ml, 3ml 0.1% in the 5ml centrifuge tube successively, each step is all removed supernatant by of short duration centrifugal (8000 changeed 30 seconds).The worm's ovum that washing is good places the ZnCl of 0.4M
2Hatching is 48 hours in the solution, and second instar larvae is collected in the 1.5ml centrifuge tube with pipettor, and is standby by of short duration centrifugal (5000 changeed 30 seconds) enrichment.
2, efficiency test method
(1) test institute's with medicament and processing
A, solid fungicide 10-20%, the disposable crop rhizosphere that imposes on when crop is transplanted;
B, positive control, 0.5% Avermectin is pressed 37.5kg/hm
2Handle;
C, blank be not with microbial inoculum or chemicals treatment.
(2) test method
At diameter is the basin alms bowl adding 500g soil sample (sick soil: sandy soil: humus soil=1: 1: 1), do above several processing of 15cm.Each is handled 5 basins and repeats for 3 times, places 25-28 ℃ greenhouse to cultivate.Transplant the back and watered, make its normal growth every 3 days.Microbial inoculum is handled the weight in wet base of the heavy and root of plant height, the acrial part of the worm's ovum number of the root knot number of investigating each processed group crop root in 30,60 days respectively in the back, rhizosphere and crop, calculates the control efficiency of microbial inoculum to root knot nematode disease.
Embodiment 1: with the sick test of microbial inoculum control tomato root-knot eelworm
Transplant 1 monthly age tomato (4-6 leaf), tomato variety is: improvement hair powder 802, agricultural science and technology development company in Shandong produces.Every basin is used the 50g microbial inoculum, carries out efficiency test by above-mentioned test method.Tabulation is depicted as the preventive effect data.
Table 1 microbial inoculum is to the control efficiency (60d) of tomato root-knot eelworm disease
The result shows that this microbial inoculum has tangible preventive and therapeutic effect to the tomato root-knot eelworm disease.
Table 2 microbial inoculum is to the influence (60d) of tomato growth
The result shows that this microbial inoculum has significant growth promoting function to the growth of tomato, apparently higher than contrast.
Embodiment 2: with the test of microbial inoculum control tobacco root knot nematode disease
Embodiment 2 methods are with embodiment 1, and difference is: transplanting tobacco bred is: Yunnan 85, Yunnan Province are educated Dohanykutato Intezet and are cultivated.
Table 3 microbial inoculum is to the control efficiency (60d) of tobacco root knot nematode disease
The result shows that this microbial inoculum has tangible preventive and therapeutic effect to the tobacco root knot nematode disease.
Table 4 microbial inoculum is to the influence (60d) of tobacco growing
The result shows that this microbial inoculum has significant growth promoting function to the growth of tobacco, apparently higher than contrast.
Embodiment 3: the preventive effect of different microbial inoculum amounts of application relatively
Embodiment 3 methods are with embodiment 1, and difference is: using microbial inoculum is every basin 50g and 75g.
The different amount of table 5 microbial inoculum is to the control efficiency % of tomato root-knot eelworm disease
The result shows that the microbial inoculum amount of application increases by 50%, and preventive effect improves 20%.
Claims (2)
1. microbial strains, it is characterized in that this microbial strains is Stropharia rugoso-annulata 2052 (Stropharia sp.2052), 2052 bacterial strains have been stored in China Microbial Culture Preservation Commission common micro-organisms center, preserving number: CGMCC No.3399 on November 5th, 2009.
2. the described micro-organisms bacterial strain of claim 1 is characterized in that Stropharia rugoso-annulata 2052 production bacterial strains are used to prevent and treat the application of root-knot nematode preparation as preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102183078A CN101703005B (en) | 2009-12-09 | 2009-12-09 | Microbial strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102183078A CN101703005B (en) | 2009-12-09 | 2009-12-09 | Microbial strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101703005A true CN101703005A (en) | 2010-05-12 |
| CN101703005B CN101703005B (en) | 2011-11-16 |
Family
ID=42373322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102183078A Expired - Fee Related CN101703005B (en) | 2009-12-09 | 2009-12-09 | Microbial strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101703005B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
| CN104928192A (en) * | 2015-06-26 | 2015-09-23 | 云南大学 | Trichoderma viride strain and application thereof |
| CN112410226A (en) * | 2020-09-10 | 2021-02-26 | 云南菌视界生物科技有限公司 | Golden stropharia rugoso-annulata strain |
| CN114574371A (en) * | 2022-03-22 | 2022-06-03 | 上海市农业科学院 | Method for degrading residual organochlorine pesticide 2, 4-dichlorophenoxyacetic acid in straws by using stropharia rugoso-annulata |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175740C (en) * | 2003-05-21 | 2004-11-17 | 云南大学 | Method of producing biology agent for killing thread worm |
| CN1225548C (en) * | 2003-06-03 | 2005-11-02 | 云南大学 | Spinous spheric structure of coprinus comatus hypha and its application |
-
2009
- 2009-12-09 CN CN2009102183078A patent/CN101703005B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
| CN101861880B (en) * | 2010-06-24 | 2012-05-09 | 云南大学 | Microbial agent and application thereof |
| CN104928192A (en) * | 2015-06-26 | 2015-09-23 | 云南大学 | Trichoderma viride strain and application thereof |
| CN112410226A (en) * | 2020-09-10 | 2021-02-26 | 云南菌视界生物科技有限公司 | Golden stropharia rugoso-annulata strain |
| CN112410226B (en) * | 2020-09-10 | 2023-02-17 | 云南菌视界生物科技有限公司 | Golden stropharia rugoso-annulata strain |
| CN114574371A (en) * | 2022-03-22 | 2022-06-03 | 上海市农业科学院 | Method for degrading residual organochlorine pesticide 2, 4-dichlorophenoxyacetic acid in straws by using stropharia rugoso-annulata |
| CN114574371B (en) * | 2022-03-22 | 2023-08-15 | 上海市农业科学院 | Method for degrading residual organic chlorine pesticide 2,4-dichlorophenoxyacetic acid in straw by utilizing stropharia rugoso-annulata |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101703005B (en) | 2011-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103271095B (en) | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof | |
| AU2019204482B2 (en) | Hydroponic method utilizing beneficial micro-organisms | |
| CN103396954B (en) | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer | |
| CN103918475A (en) | Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus | |
| ES2819255T3 (en) | Bacteria with nematicidal activity and the ability to promote plant growth | |
| CN105248233B (en) | Oil tea seedling is inoculated with the method for AM bacterium and the high-yield planting method of oil tea | |
| Declerck et al. | Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials | |
| CN101280320A (en) | Method for preparing antibiotic substance from chaetomium globosum of plant endophytic fungi | |
| CN105296366A (en) | Compound microbial agent capable of promoting tomato growth and development and application thereof | |
| CN101861880B (en) | Microbial agent and application thereof | |
| CN101703005B (en) | Microbial strain and application thereof | |
| CN106222121B (en) | A kind of bacillus megaterium bacterial strain, biocontrol agent and the preparation method and application thereof | |
| CN103087947B (en) | Pseudoxanthomonas sp. ZKB4-4 and application thereof | |
| JP5074866B2 (en) | Methods to inhibit legume feeding by pests | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN102732430A (en) | Aspergillus niger strain and application thereof | |
| CN103087946A (en) | Microbial strain and application thereof | |
| CN103113148A (en) | Culture medium for germinating boletus spore | |
| CN1545841A (en) | Method for domesticating prairie Tricholomataceae and method for cultivating the same | |
| KR100886088B1 (en) | Culturing method of hyphae containing msm and cultivating method of mushroom using its | |
| CN105613173A (en) | Methods for preventing and controlling damping off of pine seedlings through Chroogomphus rutilus | |
| CN102321538A (en) | Microbial composite inoculum and applications thereof | |
| CN109706085A (en) | No. 7 bacterial strains of one plant of Tricholoma mongolicum prairie Tricholomataceae and its acclimation method | |
| CN109161486A (en) | A kind of preparation method of biocontrol trichoderma bacteria agent and the application in tobacco planting | |
| Muthukumar et al. | Occurrence, virulence and pathogenicity of species of Pythium inciting damping-off disease in chilli |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111116 Termination date: 20121209 |