CN103113148A - Culture medium for germinating boletus spore - Google Patents
Culture medium for germinating boletus spore Download PDFInfo
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- CN103113148A CN103113148A CN2013100738069A CN201310073806A CN103113148A CN 103113148 A CN103113148 A CN 103113148A CN 2013100738069 A CN2013100738069 A CN 2013100738069A CN 201310073806 A CN201310073806 A CN 201310073806A CN 103113148 A CN103113148 A CN 103113148A
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Abstract
The invention provides a culture medium for germinating boletus spores, and the culture medium comprises a dried chroogomphus rutilus product, a PDA (Potato Dextrose Agar) culture medium and water. The invention also provides a method for preparing the culture medium and a method for germinating the boletus spores by adopting the culture medium. The culture medium provided by the invention has the advantages of simple formula, easiness for preparation, more spore germination, regular colonial morphology, thick and dense hypha, and the like.
Description
Technical field
The present invention relates to the cultural method of edible mycorrhizal fungi spore, specifically, the present invention relates to a kind of substratum be used to making the bolete spore germination and adopt the mycelial method of described culture medium culturing bolete.
Background technology
The Boletaceae (Boletaceae) of Boletus in the Basidiomycotina Agaricales, the poisonous or bitter except the minority kind and can not eating, the equal edible of most of kind.Mainly contain white, yellow, Boletus aereus.The Suillus albidipes (Peck) Sing. delicious flavour, nutritious.This bacterium thalline is larger, and meat is plump, and handle is sturdy, and food flavor is fragrant and sweet good to eat, and is nutritious, is a kind of worldwide famous edible mushrooms.It is edible that the masses of all nationalities of Yunnan Province like gathering the bright bacterium cooking.Also there is the extensively custom of edible Suillus albidipes (Peck) Sing. in every Western Europe country, and except fresh cooking, most of chip drying is processed into various small packagess, is used for preparing soup stock or makes soy sauce medicinal extract, also makes salted product edible.
Wherein, suillusgranulatus (Suillus granulatus (L.:Fr.) O.Kuntce) belongs to Suillus, in summer and autumn pine forest and mixed forest scattered, all living creatures or grow thickly on the ground.Its sporophore edible is rich in multiple nutrients, delicious flavour.Suillusgranulatus main with Chinese pine formation ectomycorrhiza, be the advantage edible mycorrhizal fungi of Pinus tabulaeformis stand.
Mycorhiza edible mushrooms (Edible mycorrhizal fungi, EMF) is to having the general designation of the macro fungi of edibleness in forest ectomycorrhiza (Ectomycorrhizal fungi, ECM fungi) fungi.After the growth needs of mycorhiza edible mushrooms and root system of plant are set up symbiotic relationship, formation symbiote mycorhiza, draw self growth desired nutritional material by mycorhiza from tree root, simultaneously provide its other nutritive substances that grow required or moisture etc. to trees again, produce sporophore when condition is suitable.A lot of famous and precious wild mushrooms are all edible mycorrhizal fungis, because the high nutritive value that they have, pharmaceutical use and important Ecology Action make it become most active field in edible mushrooms and mycorhiza research, are also to have one of challenging front subject.
Less about the research report that the mycorhiza edible mushrooms can be cultivated, only have at present Delicious lactarius forest land cultivation to succeed.The edible mushrooms spore is important reproductive material, but the sprouting of spore is different different because of the classification under it, the rare report of research of relevant suillusgranulatus spore.Mycelium after the employing spore germination is the reliable guarantee in wild mushroom domesticated strain source as bacterial classification.For the domesticating and cultivating suillusgranulatus, need to carry out artificial a large amount of cultivation bacterial classification, and find the appropriate media that makes its spore germination to be undoubtedly the important beginning of domestication.
Summary of the invention
In order to solve the above problems, the inventor has carried out continuous for many years investigation and collection to the biological habit of suillusgranulatus.On the basis that obtains pure spore, the contriver has collected the culture medium prescription of a large amount of relevant edible mycorrhizal fungi mycelium culture, and in conjunction with growing environment and the pests occurrence rule of suillusgranulatus, several formulas have been formulated, inoculation is observed repeatedly, has finally obtained being suitable for the substratum of suillusgranulatus spore germination.
The present invention is achieved by the following technical solution.
1, a kind of substratum be used to making the suillusgranulatus spore germination, wherein, described substratum comprises:
1) Gomphidius rutilus dry product;
2) PDA substratum; With
3) water.
2, substratum as described in technical scheme 1, wherein, with the weighing scale of described water, making the content of described Gomphidius rutilus dry product of the substratum of suillusgranulatus spore germination in order to preparation is 4% to 6%.
3, method as described in technical scheme 1 or 2, wherein, with the weighing scale of described water, making the content of described PDA substratum of the substratum of suillusgranulatus spore germination in order to preparation is 20% to 30%.
4, a kind of method of the described substratum of any one in compounding process scheme 1 to 3, wherein, described method comprises the steps:
1) Gomphidius rutilus boiling step: get Gomphidius rutilus, boiling 20 minutes to 40 minutes obtains the Gomphidius rutilus cooking liquor;
2) filtration step: described Gomphidius rutilus cooking liquor is filtered, obtain Gomphidius rutilus filtrate;
3) PDA substratum boiling step: use the described PDA substratum of described Gomphidius rutilus filtrate boiling, obtain be used to the described substratum that makes the bacterium spore germination.
5, method as described in technical scheme 4, wherein, described method comprises that further the described cultivation that Gomphidius rutilus boiling step is made is based on 120 ℃ to 121 ℃ sterilising treatment steps of carrying out 30 minutes sterilising treatment.
6, a kind of method that makes the bacterium spore germination, the described substratum that in described method operation technique scheme 1 to 3, the described substratum of any one or the described method of technical scheme 4 or 5 make carries out.
7, method as described in technical scheme 6, wherein, described method comprises the steps:
1) a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices step: will pour culture dish into through the described substratum of sterilising treatment;
2) spore suspension preparation process: the suillusgranulatus spore that collects is prepared into spore suspension;
3) spore smearing step: use pipettor that described spore suspension is spread upon on described culture dish; With
4) cultivate the sprouting step: described culture dish is placed in incubator cultivates, make described suillusgranulatus spore germination.
8, method as described in technical scheme 7, wherein, the concentration of described spore suspension is 10 times to 100 times diluents.
9, method as described in technical scheme 7 or 8, wherein, the applying amount of the described substratum of each described culture dish is 40 μ l to 60 μ l.
10, method as described in any one in technical scheme 7 to 9, wherein, described cultivation is sprouted step and is cultivated at the temperature of 20 ℃ to 30 ℃.
11, method as described in technical scheme 10, wherein, described cultivation is sprouted step and is comprised that successively just putting cultivation cultivates and be inverted and cultivate.
12, method as described in technical scheme 11, the wherein said incubation time of just putting is 3 days to 5 days.
The present invention has considered the culture medium prescription that germination process needs, aseptic spore suspension preparation, spore inoculating, cultivation sprouting etc., and taken into full account each link dependency each other, set up the substratum system that is applicable to the suillusgranulatus spore germination.
Substratum system of the present invention has the advantages such as formula is simple and workable, and described suillusgranulatus spore bacterial classification is easily obtained, and mycelial growth is sturdy dense, and colonial morphology is neat.Lay a good foundation for realizing the suillusgranulatus artificial domesticating cultivation.
Description of drawings
In order more to be convenient to understand the present invention, this paper provides following accompanying drawing at this, but these accompanying drawings only are used for illustration purpose, and should not be construed as for limiting the present invention, wherein:
Fig. 1 has shown that employing substratum of the present invention (M2) makes the sprouting initial stage photo of suillusgranulatus spore germination.
Fig. 2 has shown that employing substratum of the present invention (M2) makes the sprouting photo in mid-term of suillusgranulatus spore germination.
Fig. 3 has shown that employing substratum of the present invention (M2) makes the sprouting later stage photo of suillusgranulatus spore germination.
Fig. 4 has shown the 10th day after adopting substratum of the present invention (M2) to make the suillusgranulatus spore germination, will continue to cultivate resulting mycelium in the fresh M2 substratum of single bacterium colony switching.
Fig. 5 has shown the mycelial enlarged view described in Fig. 4.
Embodiment
In first aspect, the invention provides a kind of substratum be used to making the suillusgranulatus spore germination, described substratum comprises 1) the Gomphidius rutilus dry product; 2) PDA substratum; With 3) water.
Gomphidius rutilus, the Northeast is commonly called as red mushroom, pine tree umbrella, loose mushroom etc.Red mushroom belongs to Basidiomycotina Hymenomycetes, Agaricales, rivet mushroom section, Gomphidius on classification of fungi, sporophore is scattered, all living creatures or Dan Sheng.Meat fertilizer is tender, tasty, and is as famous and precious edible fungus, very popular for a long time.And the inventor finds that Gomphidius rutilus contains abundant nutritive ingredient.According to surveying and determination, the nutritive ingredient of every 100g Gomphidius rutilus dry product is roughly: protein: 18.4 grams; Fat: 0.7 gram; Carbohydrate: 58.1 grams; Food fibre: 24.6 grams; Riboflavin: 1.16 milligrams; Calcium: 14 milligrams; Phosphorus: 35 milligrams; Potassium: 169 milligrams; Sodium: 4.3 milligrams; Iron: 235.1 milligrams; Manganese: 3.75 milligrams; Zinc: 3.14 milligrams; Selenium: 91.7 milligrams; Copper: 0.51 milligram; Manganese: 3.75 milligrams.
The PDA substratum is the abbreviation (PDA, Potato Dextrose Agar) of potato dextrose agar, is made by 200g potato, 20g glucose and 20g agar, can be by commercially available acquisition.
The inventor is surprised to find that, adopts the combination of Gomphidius rutilus and PDA substratum can make the suillusgranulatus spore can be good at sprouting.
Some preferred embodiment in, with the weighing scale of described water, the weight that is used for the described Gomphidius rutilus dry product of the described substratum of boiling is 4% to 6%, is for example 4%, 5% or 6%.
Some preferred embodiment in, with the weighing scale of described water, the weight that is used for the described PDA substratum of boiling substratum of the present invention is 20% to 30%.Be preferably 22% to 28%, most preferably be 24%.For example, described content can be 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% or 30%.The weight of PDA substratum as herein described is the gross weight of potato, glucose and agar.
In second aspect, the invention provides a kind of method of preparing the above substratum, wherein, described method comprises the steps:
1) Gomphidius rutilus boiling step: get Gomphidius rutilus, boiling 20 minutes to 40 minutes obtains the Gomphidius rutilus cooking liquor;
2) filtration step: described Gomphidius rutilus cooking liquor is filtered, obtain Gomphidius rutilus filtrate;
3) PDA substratum boiling step: use the described PDA substratum of described Gomphidius rutilus filtrate boiling, obtain be used to the described substratum that makes the bacterium spore germination.
Within the preparation of PDA substratum is in the skill that those skilled in the art should have.for example, 50g Gomphidius rutilus dry product is put into the water of 1000ml, boil (for example 25 minutes to 35 minutes) about 30 minutes, filtration obtains Gomphidius rutilus and boils liquid, boil liquid 1000ml with this, put into potato fritter (soybean grain size fritter, for example 3mm to 5mmX3mm is to 5mm X3mm to 5mm fritter, as 4mm X4mm X4mm fritter) 200g, boil (for example 4 minutes to 6 minutes) about 5 minutes, filtration discards the potato residue, add agar 20g and glucose 20g, water is supplemented to 1000ml, boil and namely obtain Gomphidius rutilus to boil liquid+PDA substratum (be substratum of the present invention, for example compile in embodiment and be the M2 substratum).
Some preferred embodiment in, described method comprises that further the described cultivation that Gomphidius rutilus boiling step is made is based on 120 ℃ to 121 ℃ sterilising treatment steps of carrying out 30 minutes sterilising treatment.
In the third aspect, the present invention also provides the substratum that is made by described method.
In fourth aspect, the present invention also provides a kind of method that makes the suillusgranulatus spore germination, and described method is used above-described substratum or undertaken by the described substratum that the above method makes.
Some preferred embodiment in, make the described method of suillusgranulatus comprise the steps: 1) a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices step: will pour culture dish into through the described substratum of sterilising treatment; 2) spore suspension preparation process: the suillusgranulatus spore that collects is prepared into spore suspension; 3) spore smearing step: use pipettor that described spore suspension is spread upon on described culture dish; With 4) cultivate and sprout step: described culture dish is placed in incubator cultivates, make described suillusgranulatus spore germination.
Some preferred embodiment in, the filter paper band that comprise spore of described spore suspension for for example being collected by method of the present invention, sterile distilled water with 3ml rinses the spore on the spore band to sterile chamber, rinse colourless to band till, gained spore suspension solution is prepared into 10 times to 100 times diluents.Be for example 10,20,30,40,50,60,70,80,90 or 100 times of diluents.
Some preferred embodiment in, the applying amount of the described substratum of each described culture dish is 40 μ l to 60 μ l, is preferably 50 μ l.For example can be 40 μ l, 4l μ l, 42 μ l, 43 μ l, 44 μ l, 45 μ l, 46 μ l, 47 μ l, 48 μ l, 49 μ l, 50 μ l, 51 μ l, 52 μ l, 53 μ l, 54 μ l, 55 μ l, 56 μ l, 57 μ l, 58 μ l, 59 μ l or 60 μ l.
Some preferred embodiment in, described cultivation is sprouted step and is cultivated at the temperature of 20 ℃ to 30 ℃.In more preferred embodiments, described cultivation sprout step comprise successively just putting cultivations (be the lid of culture dish up) and be inverted cultivation (that is, and with the culture dish inversion, make lid below).Other preferred embodiment in, the described incubation time of just putting is 3 days to 5 days, is for example 3 days, 4 days or 5 days.
Explanation is in passing, and numerical range used in the present invention means to comprise end value and the interior any number of this scope and the anyon scope of this scope.
Embodiment
Hereinafter will be further detailed the present invention by embodiment, but these embodiment should not be construed as limitation of the scope of the invention only for the purpose of illustration.
The present invention's preparation used is all available from Beijing chemical reagents corporation.
The collection of suillusgranulatus spore
Gather get 250ml the day before yesterday wide-mouth glass Bottle and bottle cap several, one of bottle bottom paving circular filter paper, capping; Prepare the kraft paper of 20cm X20cm length and width or newspaper several, bungee several, 5-10, sterilization blade, scissors and tweezers.In 121 ℃ of autoclavings 30 minutes, be placed in the plastics normal temperature case with the alcohol surface sterilization standby above-mentioned article.Also the insulation can 1-2 of preparation surface alcohol disinfecting.All apparatus are the commercially available prod, there is no the specialty products of special use of the present invention.
7 to September, Pinus tabulaeformis stand select not yet to distribute spore without worm (just open in the bacterium hole, naked eyes as seen, please refer to Fig. 1 and 2) the suillusgranulatus sporophore, carefully take off cap with the sterilization blade, be placed on respectively the collecting bottle bottleneck after sterilization, cover newspaper or the kraft paper of sterilization on sporophore again, be placed in fast in clean insulation can after fixing with bungee, the temperature in insulation can keeps being no more than 15 ℃.Insulation can and plastics normal temperature case are all taken back the laboratory.
The wide-mouth vial that is placed with sporophore is placed in the refrigerator that temperature is made as 5 ℃.After 3 hours, take out filter paper under aseptic condition, be cut into band, be put in sterilization in vitro.Test tube is stored in the refrigerator that temperature is made as 5 ℃.
Smear inoculation
In Bechtop, be stored in a band that contains spore of test tube with the aseptic nipper gripping, with the sterile distilled water of 3ml, the spore on the spore bar rinsed to the aseptic triangular flask of 100ml, rinse colourless to the spore bar till, obtain spore suspension, shake up standby.Getting suspension 100 μ l joins in 900 μ l sterilized waters and namely gets 10
-1Spore suspension shakes up, and draws 10 with 100 μ l aseptic straws
-1Spore suspension is put in the 900 aseptic water pipes of μ l and is obtained 10
-2Spore suspension makes 10 successively according to identical mode
-1To 10
-5Serial dilution to 10
-5Observe at Hematocyte Counter, find that the spore suspension that is suitable for inoculating is 10
-3Spore suspension.
The spore suspension 50 μ l that draw 10 times of dilutions with aseptic pipettor join on blank substratum central position, and with the glass spatula of sterilising treatment, spore suspension is smeared out, make spore suspension be distributed in uniformly media surface, but do not scratch substratum.
Spore suspension is inoculated in respectively in 6 kinds of substratum 15 wares of every kind of culture medium inoculated.
Substratum
The formula of described 6 kinds of substratum is as follows:
M1 (/L): the PDA substratum
M2 (/L): PDA+ Gomphidius rutilus dry product 50g
M3 (/L): PDA+ wheat bran 50g
M4 (/L): the soil 100g bacterium pool, PDA+ bacterium pool soil need be carried and being soaked in water the day before yesterday, gets vat liquor and makes substratum.
M5 (/L): PDA synthetic medium (potato 200g, glucose 20g, agar 20g, KH
2PO
43g, MgSO
47H
2O1.5g, VB
1Trace)
M6 (/L): improvement MMN substratum (CaCl
22H
2O0.05g, NaCl0.025g, KH
2PO
40.5g, (NH
4)
2HPO
40.25g, MgSO
47H
2O0.15g, FeCl
3(1% solution) 1.2ml, malt meal 3g, VitB1 0.001g, glucose 10g, agar 20g, pH5.8)
Cultivate and sprout
With postvaccinal culture dish be positioned in 25 ℃ of constant incubators dark cultivate 4 days after, then it changed into being inverted cultivate, and the situations such as the time of observed and recorded spore germination, mycelia growing way, germination rate.
Found that, the spore of suillusgranulatus all can be sprouted on different culture media, but the sprouting situation is different.That spore germination needs shortest time is M6 substratum 28d, and the longest is that the M1 substratum needs 36d.15 wares for examination on M2, M3, M6 substratum have spore germination, higher than other substratum.But the colony number of sprouting in single culture dish in the M6 substratum is less than M2 and M3, and it is sparse to sprout the bacterium colony mycelia that forms.Spore all can be sprouted on M2 and M3 substratum and produce a large amount of bacterium colonies, but spore sprout time on M2 is shorter than M3, and the colonial morphology that forms is neat, and mycelia is sturdy dense.Therefore, comparatively speaking, the sprouting of M2 substratum optimum suillusgranulatus spore adopts colonial morphology that the difference of M2 substratum sprouts period as shown in Figures 1 to 3.
After spore germination the 10th day, select the single bacterium colony that grows fine in M2 substratum group and be forwarded to and continue in fresh M2 substratum to cultivate, obtain suillusgranulatus spore separation mycelium (Fig. 4 and Fig. 5).
The impact of table 1 different culture media on spore germination
Annotate: the every ware bacterium colony of+expression is sprouted number＜10, ++ represent that it is 10～20 that every ware is sprouted quantity, ++ the every ware sprouting of+expression quantity＞20; Sprout time refers to begin to the number of days that bacterium colony occurs being observed visually from the substratum that spore is spread upon culture dish.
Although described and pointed out of the present invention as being applied to the basic new feature of the preferred embodiment of the present invention, but should be understood that, those skilled in the art can carry out the form of the embodiment that sets forth and details are carried out various omissions, replacement and change and do not broken away from essence of the present invention.The present invention is not limited to embodiment described above, and these embodiments only provide for giving an example purpose, and can correct in every way in the protection domain that appended Patent right requirement limits.
Claims (10)
1. one kind is used for substratum that suillusgranulatus is sprouted, and wherein, described substratum comprises:
1) Gomphidius rutilus dry product;
2) PDA substratum; With
3) water.
2. substratum as claimed in claim 1, wherein, with the weighing scale of described water, being used for boiling, to make the weight of the described Gomphidius rutilus dry product of the substratum that suillusgranulatus sprouts be 4% to 6%.
3. substratum as claimed in claim 1 or 2, wherein, with the weighing scale of described water, being used for boiling, to make the weight of the described PDA substratum of the substratum that suillusgranulatus sprouts be 20% to 30%.
4. method of preparing the described substratum of any one in claims 1 to 3, wherein, described method comprises the steps:
1) Gomphidius rutilus boiling step: get Gomphidius rutilus, boiling 20 minutes to 40 minutes obtains the Gomphidius rutilus cooking liquor;
2) filtration step: described Gomphidius rutilus cooking liquor is filtered, obtain Gomphidius rutilus filtrate;
3) PDA substratum boiling step: use the described PDA substratum of described Gomphidius rutilus filtrate boiling, obtain be used to the described substratum that makes the bacterium spore germination.
5. method as claimed in claim 4, wherein, described method comprises that further the described cultivation that Gomphidius rutilus boiling step is made is based on 120 ℃ to 121 ℃ sterilising treatment steps of carrying out 30 minutes sterilising treatment.
6. the substratum that is made by the described method of claim 4 or 5.
7. the described substratum that method that makes the suillusgranulatus spore germination, described method right to use require the described substratum of any one in 1 to 3 or the described method of claim 4 or 5 to make carries out.
8. method as claimed in claim 7, wherein, described method comprises the steps:
1) a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices step: will pour culture dish into through the described substratum of sterilising treatment;
2) spore suspension preparation process: the suillusgranulatus spore that collects is prepared into spore suspension;
3) spore smearing step: use pipettor and glass spatula that described spore suspension is spread upon on described culture dish; With
4) cultivate the sprouting step: described culture dish is placed in incubator cultivates, make described suillusgranulatus spore germination.
9. method as claimed in claim 8, wherein, described cultivation is sprouted step and is cultivated at the temperature of 20 ℃ to 30 ℃.
10. method as claimed in claim 9, wherein, described cultivation is sprouted step and is comprised successively just putting and cultivate and be inverted and cultivate, and the preferred described incubation time of just putting is 3 days to 5 days.
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| CN103609338A (en) * | 2013-12-19 | 2014-03-05 | 云南大学 | Brick-red xerocomus silwoodensis test tube sporocarp primordium inducing method |
| CN104370632A (en) * | 2014-11-06 | 2015-02-25 | 丽水市农业科学研究院 | Culture medium for improving separation survival rate of boletus mycelium |
| CN105039178A (en) * | 2015-08-13 | 2015-11-11 | 云南省热带作物科学研究所 | Phlebopus portentosus basidiospore germination method |
| CN117887595A (en) * | 2024-03-14 | 2024-04-16 | 云南省林业和草原科学院 | Phlebopus portentosus YAFMF008, separation method thereof and mycorrhizal seedling infection method |
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| CN101665379A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Culture medium for fermenting boletus liquid |
| CN102511302A (en) * | 2011-11-10 | 2012-06-27 | 云南省热带作物科学研究所 | Phlebopus portentosus cultivation fungus stick |
| CN102687641A (en) * | 2012-06-25 | 2012-09-26 | 苏州百趣食品有限公司 | Method for producing mycelia by liquid fermentation of gomphidius rutilus |
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| JP2009240276A (en) * | 2008-03-31 | 2009-10-22 | Naris Cosmetics Co Ltd | Liquid culture medium for boletaceae suillus basidiomycete |
| CN101665379A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Culture medium for fermenting boletus liquid |
| CN102511302A (en) * | 2011-11-10 | 2012-06-27 | 云南省热带作物科学研究所 | Phlebopus portentosus cultivation fungus stick |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609338A (en) * | 2013-12-19 | 2014-03-05 | 云南大学 | Brick-red xerocomus silwoodensis test tube sporocarp primordium inducing method |
| CN103609338B (en) * | 2013-12-19 | 2015-09-30 | 云南大学 | The abductive approach of brick red suede lid bolete test tube fruit body primordium |
| CN104370632A (en) * | 2014-11-06 | 2015-02-25 | 丽水市农业科学研究院 | Culture medium for improving separation survival rate of boletus mycelium |
| CN104370632B (en) * | 2014-11-06 | 2017-06-30 | 丽水市农业科学研究院 | It is a kind of to improve the culture medium that bolete mycelium separates survival rate |
| CN105039178A (en) * | 2015-08-13 | 2015-11-11 | 云南省热带作物科学研究所 | Phlebopus portentosus basidiospore germination method |
| CN117887595A (en) * | 2024-03-14 | 2024-04-16 | 云南省林业和草原科学院 | Phlebopus portentosus YAFMF008, separation method thereof and mycorrhizal seedling infection method |
| CN117887595B (en) * | 2024-03-14 | 2024-05-17 | 云南省林业和草原科学院 | Phlebopus portentosus YAFMF008, separation method thereof and mycorrhizal seedling infection method |
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| Publication number | Publication date |
|---|---|
| CN103113148B (en) | 2014-10-22 |
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