CN103087946A - Microbial strain and application thereof - Google Patents
Microbial strain and application thereof Download PDFInfo
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- CN103087946A CN103087946A CN2012105803229A CN201210580322A CN103087946A CN 103087946 A CN103087946 A CN 103087946A CN 2012105803229 A CN2012105803229 A CN 2012105803229A CN 201210580322 A CN201210580322 A CN 201210580322A CN 103087946 A CN103087946 A CN 103087946A
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Abstract
The invention relates to a microbial strain and application thereof, which belong to the technical field of microbial pesticides. The microbial strain is Streptomyces fimicarius D153. The D153 strain has been preserved in a general microorganism center of the China Committee for Culture Collection of Microorganisms since July 18th, 2012, and the preservation number of the D153 strain is CGMCC No.6353. The microbial strain is applied to preparation of a root-knot nematode control reagent. The greenhouse control effect of microbial inoculum prepared from the microbial strain to the root-knot nematode disease of tomatoes is higher than 60%, the greenhouse control effect of the microbial inoculum to the root-knot nematode disease of tobaccos is higher than 40%, and plants can be obviously promoted to grow. Therefore, the root-knot nematode control reagent prepared from the microbial strain has the advantages of high efficiency, low toxicity and no residue.
Description
Technical field:
The present invention relates to a kind of microorganism strains and application thereof, the microorganism belonging to genus technical field of pesticide.
Technical background:
Plant nematode is one of disease that generally occurs on crop, endangers nearly all crop.Only the annual loss that causes in the whole world of root knot nematode (Meloidogyne spp.) is just up to tens billion of dollars.Owing to producing the upper resistant variety that lacks, the farm crop multiple crop index is high, and the use of nematocide is restricted, and the needs of Sustainable Development of Modern Agriculture, and the biological control Plant nematode comes into one's own day by day.
Actinomycetes are as a kind of Microbial resources of uniqueness, and its abundant meta-bolites has diversified activity.This patent utilizes the distinctive Biological resources in Yunnan, filters out to produce the actinomycetes strain that efficiently poisoning nematode active metabolite is killed root knot nematode, has potential and actual using value in the biological control of nematode.
Summary of the invention:
The object of the present invention is to provide a kind of microorganism strains and application thereof.
The present invention specifically is achieved in that
The contriver is in early-stage Study, and screening has obtained to have actinomycetes streptomyces fimicarius (Streptomyces fimicarius) D153 of good nematode killing function.The D153 bacterial strain has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 12nd, 2012; Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica; Preserving number is CGMCC No.6353.
Streptomyces fimicarius (Streptomyces fimicarius) fibrillae of spores is straight or flexible, spore is Long Circle, cylindricality or shaft-like, smooth surface, when cultivating on synthetic medium, the little brown band of bacterium colony blush is transferred or light-yellow tinge, but lysochrome is brown or tawny, and aerial hyphae is dark dark brown or little grey (Taguchi, 1993).
Streptomyces fimicarius D153 bacterial strain of the present invention is as the application for the preparation of control root knot nematode preparation.
The present invention cultivates streptomyces fimicarius D153 by conventional liquid bulk, obtain to comprise the bacterium liquid of thalline and exocytosis thing, the use of spreading manuer in holes.
This actinomycete agent can mix use with other nematicide agricultural chemicals, plant-growth regulator, but must guarantee that institute's confection has no side effect to the microbial inoculum that the present invention adopts.
Actinomycete agent of the present invention is generally used when crop sowing or transplanting.Method is during when sowing and seed are manured into soil jointly, during transplanting, this preparation to be applied around crop rhizosphere.Serious area is occured nematode can impose in process of crop growth once, and method is that this preparation is applied around crop rhizosphere.
The present invention compares with the existing plant nematode medicine that kills, and has advantages of efficient, low toxicity and noresidue.
Embodiment:
The fermentation method for producing of the compounds of this invention (ordinary method):
1, liquid seeds is cultivated: the Gause I liquid nutrient medium divides and installs in the 500ml triangular flask, conventional sterilization, again on Bechtop with inoculating needle from the test tube slant some mycelium inoculations of picking in wherein, then 22-28 ℃, 150-180rpm/min cultivate on shaking table 5-8 days standby.
The seed that 2, will shake is inoculated in fermentor tank by the inoculum size of 10-20%, controls temperature at 22-28 ℃, cultivates 2-3 days, then the fermented liquid water is regulated, and makes CFU 10
5-10
8, standby.
The efficiency test of preparation of the present invention to root knot nematode:
1, the cultivation of Meloidogyne incognita (Meloidogyne incognita)
The tomato root with Meloidogyne incognita of hot-house culture is cleaned with sterilized water, the egg capsule of dissecting needle picking root knot nematode is placed in the KCl solution of 0.4M, use successively NaClO solution washing and the sterilization of 4ml sterilized water (three times), the two anti-solution of the aseptic green grass or young crops-Streptomycin sulphate of 1ml, 3ml 0.1% in the 5ml centrifuge tube, each step is all removed supernatant liquor by of short duration centrifugal (8000 turn 30 seconds).The worm's ovum that washing is good is placed in the ZnCl of 0.4M
2In solution, hatching is 48 hours, and second instar larvae is collected in the 1.5ml centrifuge tube with pipettor, and is standby by of short duration centrifugal (5000 turn 30 seconds) enrichment.
2, efficiency test method
(1) test institute's with medicament and processing
A, liquid bacterial agent, the disposable crop rhizosphere that imposes on when crop is transplanted;
B, positive control, 0.5% Avrmectin is by 37.5 kg/hm
2Process;
C, blank be not with microbial inoculum or chemicals treatment.
(2) test method
The basin alms bowl that is 15 cm at diameter adds 500g soil sample (sick soil: sandy soil: vegetable mould=1:1:1), do above several processing.Each is processed 5 basins and repeats for 3 times, and the greenhouse that is placed in 25-28 ℃ is cultivated.Watered every 3 days after transplanting, make its normal growth.Investigate respectively the weight in wet base of the heavy and root of plant height, the over-ground part of the worm's ovum number of root knot number, rhizosphere of each treatment group crop root and crop after microbial inoculum is processed in 30,60 days, calculate microbial inoculum to the prevention effect of root knot nematode disease.
Embodiment 1: with the sick test of microbial inoculum control tomato root-knot eelworm
Transplant 1 monthly age tomato (4-6 leaf), tomato variety is: in assorted No. 9, Shandong agricultural science and technology development company produces.Every basin is used the 100ml microbial inoculum, carries out efficiency test by above-mentioned test method.List is depicted as the preventive effect data.
The prevention effect (60d) of table 1 microbial inoculum to the tomato root-knot eelworm disease
Result shows, this microbial inoculum has obvious preventive and therapeutic effect to the tomato root-knot eelworm disease.
The impact (60d) of table 2 microbial inoculum on tomato growth
Result shows, this microbial inoculum has significant growth promoting function to the growth of tomato, apparently higher than contrast.
Embodiment 2: with the test of microbial inoculum control tobacco root-knot
Embodiment 2 methods are with embodiment 1, and difference is: transplanting tobacco bred is: Yunnan 85, Yunnan Province are educated Dohanykutato Intezet and are cultivated.
The prevention effect (60d) of table 3 microbial inoculum to tobacco root-knot
Result shows, this microbial inoculum has obvious preventive and therapeutic effect to tobacco root-knot.
The impact (60d) of table 4 microbial inoculum on tobacco growing
Result shows, this microbial inoculum has significant growth promoting function to the growth of tobacco, apparently higher than contrast.
Embodiment 3: the field efficacy of different microbial inoculum amounts of application
Embodiment 3 methods are with embodiment 1, and difference is: using microbial inoculum is every basin 30ml, 100ml and 180ml.
The prevention effect % of the different amount of table 5 microbial inoculum to the tomato root-knot eelworm disease
| Amount of application | 30d | 60d |
| 30ml | 30.76 | 52.63 |
| 100ml | 69.24 | 52.63 |
| 180ml | 53.84 | 31.58 |
| Positive control | 69.24 | 68.42 |
| Blank | - | - |
Result shows, when using dosage was 100ml, prevention effect was best.
Claims (2)
1. a microorganism strains, is characterized in that this microorganism strains is streptomyces fimicarius (Streptomyces fimicarius) D153, and preserving number is: CGMCC No.6353.
2. streptomyces fimicarius claimed in claim 1 (Streptomyces fimicarius) D153 bacterial strain is for the preparation of the application in control root knot nematode preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105803229A CN103087946A (en) | 2012-12-28 | 2012-12-28 | Microbial strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105803229A CN103087946A (en) | 2012-12-28 | 2012-12-28 | Microbial strain and application thereof |
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| Publication Number | Publication Date |
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| CN103087946A true CN103087946A (en) | 2013-05-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012105803229A Pending CN103087946A (en) | 2012-12-28 | 2012-12-28 | Microbial strain and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106336329A (en) * | 2016-08-29 | 2017-01-18 | 佛山市艳晖生物科技有限公司 | Microbial bacterial fertilizer special for honeysuckles and preparation method thereof |
| CN108208016A (en) * | 2017-12-31 | 2018-06-29 | 海南大学 | Application of the volatile materials that streptomyces fimicarius generates in control of plant disease |
| CN113832063A (en) * | 2021-09-29 | 2021-12-24 | 海南大学 | Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum |
| CN117264851A (en) * | 2023-11-10 | 2023-12-22 | 中国热带农业科学院三亚研究院 | Streptomyces strain and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3116219A (en) * | 1961-08-21 | 1963-12-31 | American Cyanamid Co | Microbiogical 16alpha-hydroxylation of steroids |
| CN101508968A (en) * | 2009-03-06 | 2009-08-19 | 中国农业大学 | Biological medicament for preventing and treating plant parasitic nematode and uses thereof |
| CN102358887A (en) * | 2011-08-31 | 2012-02-22 | 云南大学 | Streptomyces fimicarius for eliminating toxicity of jatropha curcas cake |
-
2012
- 2012-12-28 CN CN2012105803229A patent/CN103087946A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3116219A (en) * | 1961-08-21 | 1963-12-31 | American Cyanamid Co | Microbiogical 16alpha-hydroxylation of steroids |
| CN101508968A (en) * | 2009-03-06 | 2009-08-19 | 中国农业大学 | Biological medicament for preventing and treating plant parasitic nematode and uses thereof |
| CN102358887A (en) * | 2011-08-31 | 2012-02-22 | 云南大学 | Streptomyces fimicarius for eliminating toxicity of jatropha curcas cake |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106336329A (en) * | 2016-08-29 | 2017-01-18 | 佛山市艳晖生物科技有限公司 | Microbial bacterial fertilizer special for honeysuckles and preparation method thereof |
| CN108208016A (en) * | 2017-12-31 | 2018-06-29 | 海南大学 | Application of the volatile materials that streptomyces fimicarius generates in control of plant disease |
| CN113832063A (en) * | 2021-09-29 | 2021-12-24 | 海南大学 | Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum |
| CN113832063B (en) * | 2021-09-29 | 2023-11-24 | 海南大学 | Preparation method and application of Streptomyces faecalis BWL-H1 microbial inoculum |
| CN117264851A (en) * | 2023-11-10 | 2023-12-22 | 中国热带农业科学院三亚研究院 | Streptomyces strain and application thereof |
| CN117264851B (en) * | 2023-11-10 | 2024-02-09 | 中国热带农业科学院三亚研究院 | Streptomyces strain and application thereof |
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Application publication date: 20130508 |