CN101692877A - Diet regulating and controlling method for producing mutton enriched with conjugated linoleic acid - Google Patents
Diet regulating and controlling method for producing mutton enriched with conjugated linoleic acid Download PDFInfo
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- CN101692877A CN101692877A CN200910236442A CN200910236442A CN101692877A CN 101692877 A CN101692877 A CN 101692877A CN 200910236442 A CN200910236442 A CN 200910236442A CN 200910236442 A CN200910236442 A CN 200910236442A CN 101692877 A CN101692877 A CN 101692877A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a diet regulating and controlling method for producing mutton enriched with conjugated linoleic acid. The method adopts concentrate feed, roughage, oil seeds and activated charcoal to form diet for feeding sheep 5-12 month old, wherein the mass ratio of the concentrate feed and the roughage is 4:6-5:5, and the mass of oil seeds is 7%-12% of the sum of the masses of the concentrate feed and the roughage, and the mass of the active carbon is 2%-10% of the mass of the concentrate feed. As sheep are fed through the method, the content of cis-9, trans-11C18:2 conjugated linoleic acid in mutton obtained after slaughtering the sheep is improved above 80% on the average compared with mutton of sheep with the same variety and age which are fed by the common method, conjugated linoleic acid in mutton mainly comes from a generation path in sheep bodies. The method is simple and easy to operate, has lower production cost, and is easy to popularize and apply.
Description
Technical field
The present invention relates to a kind of production and be rich in the diet regulating and controlling method of CLA mutton.
Background technology
(conjugated linoleic acid CLA) is meant a series of mixtures that contain conjugated double bond, have the position octadecadienoic acid isomer different with steric configuration to CLA, is a kind of natural active matter.The kind of CLA is very abundant, has 24 kinds of isomers at least, and in these isomers, the conjugated linoleic acid isomers that human body is had the physiological hygiene effect mainly is: suitable-9, anti--11-grips linoleic acid altogether.The milk of ruminant domestic animal such as ox, sheep, meat product are the main sources that the mankind obtain CLA.
Studies show that, in the ruminant body, exist two kinds to generate the CLA approach.The one, cud generates approach, and promptly some bacterium in the cud can change into CLA with the linoleic acid in the feed; The 2nd, the endogenous route of synthesis of mammary gland and adipose tissue, promptly the linoleic acid in the feed, linolenic acid etc. after the intermediate product trans-11C18:1 (anti--110 eight carbon monoenoic acids) that cud generates is absorbed, the Δ in mammary gland and adipose tissue
9Dehydrogenation forms and grips linoleic acid altogether under the effect of-dehydrogenase.The CLA that deposits in the ruminant domestic animal soma is mainly derived from endogenous route of synthesis.
Research confirms that suitable-9, anti--11-CLA has the accumulation of the body fat of inhibition, antiatherosclerosis, enhancing body immunity, suppresses human tumor, improves bone density, regulates multiple important physiological function such as blood sugar and blood pressure.Research data shows, human body minimum absorption CLA 3 grams that need every day that body weight is 70 kilograms.Yet, even in dairy products and the higher western countries of animal product intake, CLA reality per capita daily intaking amount also less than 1/3rd of above-mentioned recommended amounts.Therefore, the mutton that is rich in CLA has vast market prospect.The mutton that this patent provided, the content of CLA is at 8.4-12.8mg/g TFA (TFA).Through State Intellectual Property Office's patent retrieval, not seeing both at home and abroad has the patent of producing the diet regulating and controlling method that is rich in CLA mutton.
According to the result in State Intellectual Property Office's patent retrieval, the patent No. 200610098409.7, denomination of invention are " a kind of milk that contains CLA ".There is similarity on this invention and the surface of the present invention, but on theoretical basis and technological means, has essential different.The patent No. 200610098409.7, invention are taked two kinds of preparation methods, first method on technological means be to add a certain proportion of mixing CLA contains CLA with preparation milk in conventional milk; Second method is directly to be obtained by the milk cow secretion by the method for breeding that improves milk cow.The method for breeding that improves milk cow is to add sunflower oil 150-250g, fish oil 150-250g, linoleic acid 150-250g, leukotrienes 30-80g active yeast powder 50g in milk cow forage, perhaps every day, perfusion mixed CLA 150-250g, Vitwas E 1.5g, vitamin C 12g.
Summary of the invention
The purpose of this invention is to provide a kind of production and be rich in the diet regulating and controlling method of CLA mutton.The sheep of adopting this method to feed can obtain being rich in the mutton of CLA (content is at 8.4-12.8mg/g) after butchering.
The daily ration of sheep provided by the present invention is made up of concentrated feed, roughage, oil plant seed and active carbon; Wherein, the mass ratio of concentrated feed and roughage is 4 in the described daily ration: 6-5: 5, the quality of described oil plant seed is the 7%-12% of described concentrated feed and roughage quality sum, the quality of described active carbon is the 2%-10% of described concentrated feed quality, and the quality of described roughage and the quality of described concentrated feed are all in butt.
Reaching under the prerequisite of same feeding effect, from saving cost consideration, the quality optimization of active carbon is the 2%-5% of described concentrated feed quality in the daily ration of the present invention.
The oil plant seed can be linseed and/or sunflower seeds described in the daily ration of the present invention.
Described activated carbon is the food-grade active carbon, preferably apricot shell activated carbon, and granularity is that the content of apricot shell active carbon of 400-700um is greater than 85% in the described apricot shell active carbon.
Roughage in roughage in the daily ration of the present invention and concentrated feed and the existing daily ration is identical with the composition of concentrated feed; As: roughage can be made up of the material of following mass percent: grass family green hay 20%-30%, alfalfa hay 20%-30% and ear corn ensiling 40%-60%; Concentrated feed can be made up of the material of following mass percent: corn 63%-75%, dregs of beans 20%-35%, salt 2.13%-3.51%, premix 2.3%-2.7%.
Adopt the above-mentioned daily ration sheep at 5-12 monthly age of feeding, preferably fed 60-90 days, can obtain being rich in the mutton of CLA after butchering.
Feed before the daily ration of the present invention, the body weight of described sheep is preferably 20kg-35kg.Between feed period, feed every day twice of daily ration of every sheep.
In the mutton of producing with said method, cis-9, trans-11C18:2 grip linoleic acid content altogether than the same kind of producing with conventional method, on average can improve more than 80% with age in days mutton.The CLA that is deposited in this mutton is mainly derived from generation approach in the sheep body.
The supply level of other nutrition can provide according to NRC (1985) mutton sheep feeding standard between feed period.
The polyunsaturated fatty acid that method of the present invention is based on daily ration forms a certain amount of intermediate product through hydrogenation, isomate process in the sheep cud---and trans-11C18:1 (anti--110 eight carbon monoenoic acids), the further hydrogenation of trans-11C18:1 forms the end-product stearic acid.After intermediate product trans-11C18:1 is absorbed and runs to adipose tissue, generate cis-9 under the effect of Δ 9-dehydrogenase, trans-11C18:2 grips linoleic acid altogether.It is many more that trans-11C18:1 absorbs, cis-9 in the suet fat tissue, and it is high more that trans-11C18:2 grips linoleic content altogether.In daily ration, add the oil plant seed that is rich in polyunsaturated fatty acids such as linoleic acid, linolenic acid; can increase considerably the output of trans-11C18:1 in the cud; and active carbon has the effect of absorption, protection trans-11C18:1; reduce the quantity of its further hydrogenation of quilt in cud; making more trans-11C18:1 enter small intestine is absorbed; for adipose tissue provides more generation cis-9, trans-11C18:2 grips linoleic substrate altogether.
Method of the present invention has the following advantages:
(1) though the mutton that conventional method is produced contains a certain amount of CLA, and content is low, and health-care efficacy is not obvious, the present invention can improve the content of the CLA in the mutton more than 80%, and the health care of mutton is significantly improved;
(2) compare with the feed of independent interpolation oilseeds, in feed, add an amount of oilseeds and active carbon simultaneously, more trans-11C18:1 is absorbed, further improve the content of CLA in the mutton;
(3) method of the present invention is simple, and production cost is lower, is easy to apply.
The specific embodiment
Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
Embodiment 1, different types of active carbon adsorb the ability of LCFA in cud
1, experimental animal:
Acerous road Saite sheep (♂) * little tail sheep (♀) first-filial generation ram that trembles with fear is installed permanent fistula
2, the kind of active carbon:
AC1 raw material: apricot shell (active carbon Co., Ltd of the brilliant section of Beijing brilliance, model GH-11)
Granularity: 400-700 micron (28-42 order) is 85% (quality percentage composition)
Preparation method: physical activation method
AC2 raw material: apricot shell (active carbon Co., Ltd of the brilliant section of Beijing brilliance, model GH-15)
Granularity: 400-700 micron (28-42 order) 〉=85% (quality percentage composition)
Preparation method: physical activation method
AC3 raw material: coconut husk (active carbon Co., Ltd of the brilliant section of Beijing brilliance, model GH-12)
Granularity: 180-450 micron (40-80 order) 〉=90% (quality percentage composition)
Preparation method: physical activation method
3, daily ration is formed
The test daily ration is as shown in table 1.Wherein, oilseeds add at concentrated feed and sneak into fine fodder with mixer man-hour after fragmentation; The mass ratio of fine fodder and active carbon is 30: 1; Contain in every kilogram of premix: mass content is 1% KI 4.89g, FeSO
4H
2O 6.39g, CuSO
45H
2O 1.77g, mass content is 1%CoCl
20.96g, MnSO
4H
2O3.24g, ZnSO
4H
2O 4.11g, mass content is 1%Na
2SeO
31.65g, bentonite 276.99g, corn flour 700g.
Table 1 test daily ration is formed (%, dry matter basis)
4, feeding and management:
Daily ration every day 08:00 and 15:00 divide and feed for 2 times, feed roughage earlier, concentrated feed is fed in the back.Between feed period, freely drink water, freely lick block salt.
5, measure different types of active carbon with the Nylon Bag method and in cud, adsorb the ability of LCFA, thereby filter out the activated carbon product that trans-11C18:1 is had good selective absorption function.
The extraction of aliphatic acid
1, accurately takes by weighing 200-300mg freeze-drying sample (fatty 20-50mg) in the tool plug test tube of 25ml.
2, add mark (C19:0) in the 5ml in test tube, 4ml gathers oxyacetone, tightens lid, and 1min vibrates on oscillator.
3, test tube is put into 80 ℃ of water-baths of thermostat water bath 2 hours, after the taking-up cooling, the adding mass concentration is 7%K
2CO
3Solution 7ml vibrated one minute, left standstill, and got supernatant and measured.
Adopt gas chromatography to measure the content of fatty acid of charcoal absorption, with gas chromatograph HP6890 (Hewlett-Packard, Avondale, PA, the U.S.).Measure condition of work: adopt 60m CP-Sil 88 capillary chromatographs (Varian, Walnut Creek, CA, the U.S.), carrier gas is high-purity helium, 250 ℃ of injection temperatures, 250 ℃ of detected temperatures; Temperature program(me) is 180 ℃ and keeps 15mi that the speed with 4 ℃/min rises to 220 ℃ then, 220 ℃ of maintenance 8min.In be designated as C19:0 (Sigma-Aldrich, St.Lousis, MO, the U.S.).The result is as shown in table 2, and the content of C18:0, C18:1, C18:2 all is higher than other processed group among the AC2.
The composition (mg/gTFA) of table 2 variety classes charcoal absorption aliphatic acid
The adding proportion of embodiment 2, different activities charcoal is adsorbed the ability of LCFA in cud
Adopt AC2 (raw material: apricot shell, granularity: in the test below 400-700 micron (28-42 order) 〉=85%) as adsorbent.
1, experimental animal: the same first step.
2, daily ration is formed: the same first step.
Activated carbon dosage is as shown in table 3 below.
The ratio of table 3 fine fodder and active carbon
3, feeding and management: with embodiment 1.
Measure the different proportion active carbon adsorbs LCFA in cud ability with the Nylon Bag method.
Adopt the assay method mensuration active carbon identical with embodiment 1 and the content of the CLA in the rumen content, the result is as shown in table 4, and the value of 20: 1 active carbon C18:0/C18:1/ cud C18:0/C18:1 of processed group is littler than other groups.
Table 4 different proportion active carbon is to the influence of active carbon C18:0/C18:1/ cud C18:0/C18:1
Can reach a conclusion by top test, (fine fodder: in the time of active carbon), active carbon was the strongest to the adsorption capacity of LCFA in the cud at 20: 1 for the adding proportion of active carbon.
The feed of embodiment 3, feed interpolation sunflower seeds and active carbon
1, the screening of mutton sheep
Kind: Suffolk (♂) * little tail sheep (♀) first-filial generation ram that trembles with fear
Body weight: 28.15 ± 0.7kg
Sheep age: 4 monthly ages
2, daily ration is formed
The test daily ration is as shown in table 5.Wherein oilseeds add at concentrated feed and sneak into fine fodder with mixer man-hour after fragmentation.
Table 5 test daily ration is formed and trophic level (% dry matter basis)
3, feeding and management: with embodiment 1.
Through the hybridization mutton sheep that said method is raised, after 2 months, butcher, promptly obtain being rich in the mutton of CLA.
Adopt the assay method identical with embodiment 1 operation to measure the content of soma's CLA, the result is as shown in table 6.In sheep body longissimus dorsi muscle, gluteus, the subcutaneous fat suitable-9, instead-11 grip linoleic content interpolation oilseeds group altogether and improved 64.5%, 77.1% and 90.6% respectively than conventional method for breeding (blank group), cla levels has improved 21.44%, 17.24%, 15.59% respectively in the tissue of the above-mentioned position of oilseeds group than only adding to add oilseeds and active carbon group simultaneously.
Suitable in table 6 mutton-9, anti--11 grip linoleic acid content (mg/gTFA) altogether
Claims (8)
1. the daily ration of a sheep is made up of concentrated feed, roughage, oil plant seed and active carbon; Wherein, the mass ratio of concentrated feed and roughage is 4 in the described daily ration: 6-5: 5, the quality of described oil plant seed is the 7%-12% of described concentrated feed and roughage quality sum, the quality of described active carbon is the 2%-10% of described concentrated feed quality, and the quality of described roughage and the quality of described concentrated feed are all in butt.
2. daily ration according to claim 1 is characterized in that: the quality of described active carbon is the 2%-5% of described concentrated feed quality.
3. daily ration according to claim 1 and 2 is characterized in that: described oil plant seed is linseed and/or sunflower seeds.
4. according to arbitrary described daily ration among the claim 1-3, it is characterized in that: described activated carbon is an apricot shell activated carbon, and granularity is that the content of apricot shell active carbon of 400-700um is greater than 85% in the described apricot shell active carbon.
5. the method for the sheep of feeding is to adopt among the claim 1-4 arbitrary described daily ration 5-12 monthly age sheep of feeding.
6. method according to claim 5 is characterized in that: before the described daily ration of feeding, the body weight of described 5-12 monthly age sheep is 20kg-35kg.
7. require 5 or 6 described methods according to power, it is characterized in that: the fate of the described 5-12 of feeding monthly age sheep is 60-90 days.
8. according to arbitrary described method among the claim 5-7, it is characterized in that: between feed period, feed every day twice of described daily ration of every sheep.
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| CN2009102364425A CN101692877B (en) | 2009-10-22 | 2009-10-22 | Diet regulating and controlling method for producing mutton enriched with conjugated linoleic acid |
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| CN2009102364425A CN101692877B (en) | 2009-10-22 | 2009-10-22 | Diet regulating and controlling method for producing mutton enriched with conjugated linoleic acid |
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| CN101692877A true CN101692877A (en) | 2010-04-14 |
| CN101692877B CN101692877B (en) | 2012-02-01 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856060A (en) * | 2010-05-28 | 2010-10-13 | 中国科学院东北地理与农业生态研究所 | Sheep ration for increasing conjugated linoleic acid (CLA) and precursor thereof in mutton |
| CN101953431A (en) * | 2010-10-15 | 2011-01-26 | 吴建平 | Feed capable of improving CLA content in meat products |
| CN107259110A (en) * | 2017-08-01 | 2017-10-20 | 合肥合丰牧业有限公司 | It is a kind of effectively to lift the feed addictive of cla levels in mutton |
| CN107259120A (en) * | 2017-06-26 | 2017-10-20 | 兰州大学 | A kind of method that utilization linseed diet produces function mutton |
| CN107455674A (en) * | 2016-06-02 | 2017-12-12 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Improve quality of mutton, the production method of cla levels and its mutton sheep Ration Compound method |
| CN108935321A (en) * | 2018-08-02 | 2018-12-07 | 四川科为民生物技术有限公司 | One kind being rich in CLA nutrition swine rearing method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002351537A1 (en) * | 2002-10-31 | 2004-06-18 | Piotr Zajac | Feed additive and process for manufacturing thereof |
| CN1224332C (en) * | 2004-02-20 | 2005-10-26 | 中国农业科学院畜牧研究所 | Method for increasing output of trans-11 oleic acid from rumen |
| CN1709126A (en) * | 2005-06-23 | 2005-12-21 | 刘蒙榕 | Active-carbon efficient environmental-protection fodder |
-
2009
- 2009-10-22 CN CN2009102364425A patent/CN101692877B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856060A (en) * | 2010-05-28 | 2010-10-13 | 中国科学院东北地理与农业生态研究所 | Sheep ration for increasing conjugated linoleic acid (CLA) and precursor thereof in mutton |
| CN101953431A (en) * | 2010-10-15 | 2011-01-26 | 吴建平 | Feed capable of improving CLA content in meat products |
| CN107455674A (en) * | 2016-06-02 | 2017-12-12 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Improve quality of mutton, the production method of cla levels and its mutton sheep Ration Compound method |
| CN107259120A (en) * | 2017-06-26 | 2017-10-20 | 兰州大学 | A kind of method that utilization linseed diet produces function mutton |
| CN107259110A (en) * | 2017-08-01 | 2017-10-20 | 合肥合丰牧业有限公司 | It is a kind of effectively to lift the feed addictive of cla levels in mutton |
| CN108935321A (en) * | 2018-08-02 | 2018-12-07 | 四川科为民生物技术有限公司 | One kind being rich in CLA nutrition swine rearing method |
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| CN101692877B (en) | 2012-02-01 |
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