CN102334604A - Application of copper threonate - Google Patents
Application of copper threonate Download PDFInfo
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- CN102334604A CN102334604A CN2011101956926A CN201110195692A CN102334604A CN 102334604 A CN102334604 A CN 102334604A CN 2011101956926 A CN2011101956926 A CN 2011101956926A CN 201110195692 A CN201110195692 A CN 201110195692A CN 102334604 A CN102334604 A CN 102334604A
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Abstract
The invention discloses application of a threonine chelate named as copper threonate. The copper threonate can be used as an additive for an animal feed, so that the aims of effectively promoting growth of animals, enhancing resistance of the animals and improving the utilization efficiency of the feed are fulfilled.
Description
The application divides an application, and the application number of its original bill application is 200910043299.8, and the applying date is on 05 05th, 2009, and denomination of invention is " copper-threonine ".
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof.Relate in particular to a kind of amino-acid chelate.
Background technology
Threonine is a kind of important nutrition fortifier, can play the fortification effect to cereal, cake, dairy products etc.Threonine is the same with tryptophan, also has to recover human-body fatigue, promote the function of growing.Pharmaceutically,, human body skin is had about water holding, after oligonucleotide chain combines, can play an important role the protection cell membrane owing to contain hydroxyl in the structure of threonine; Threonine can also promote the synthetic and fatty acid oxidation of phosphatide in vivo, and its preparation has medicinal efficacies such as the human development of promotion, AFL, uses always and makes a constituent in the compound amino acid transfusion.Simultaneously, threonine is still made efficient low irritated antibiotic---the base stock of monobactam.The Along with people's living standard propose the continuous development of doing with aquaculture, threonine is used amino acid as feed, has been widely used in to add pig starter feed, plant pig feed, broiler fodder, prawn feed and eel feed etc.Threonine has following characteristics as animal feed additive: can adjust amino acid whose balance in the feed, promote growth of animal; Can improve meat; Can improve the nutritive value of the low animal feed raw material of amino acid digestibility; Can be used for producing the feed of low albumen, help to practice thrift protein resource; Can reduce the animal feed cost of material; Can reduce ammonia concentration and rate of release in nitrogen content in feces of livestock and poultry and the urine, the livestock and poultry house.Threonine is as the common a kind of limiting amino acid of animal, and its content and proportioning directly affect the nutritive value of daily ration and the production performance of livestock and poultry, in the animal daily ration, adds limiting amino acid and can improve efficiency of feed utilization to greatest extent.
Trace copper can effectively be participated in the important physiological process such as pigmentation and the formation of spinal cord myelin of formation, keratinization and tissue of cellular oxidation, bone and the connective tissue of body, and keeps normal cardiac function to have important function to body.Copper also is the essential component of some enzyme systems, and especially relevant with cellular oxidation metalloenzyme is participated in organism metabolism directly.The absorption and the function of copper and iron are closely related, can keep the eubolism of ferro element, help the synthetic and erythrocytic maturation of hemoglobin; Can also participate in the breeding activity of body through promoting hypothalamus secretion luteinising hormone-releasing hormo.As human body and animal trace elements necessary; At present inorganic salts that adopt copper as animal feed additive more, and animal absorbs these inorganic salts must pass through the various biological barrier, and its absorptivity is low; Addition is big; Biological value is low, most of discharges also contaminated environment of back through ight soil, and the activity of other nutritional labelings in the feed (like vitamin etc.) is had destructive effects. employing solid threonine and inorganic copper are also arranged jointly as the situation of feed addictive; But operating process is numerous and diverse, and the dissolubility of solid threonine is relatively more difficult.
The advantage that how to combine above-mentioned nutritional labeling and trace element better; The complementarity of two kinds of materials can be found full expression in feed; And better promote the synchronous absorption of animal body to above-mentioned two kinds of materials just to become the technical problem that those skilled in the art need to be resolved hurrily.
Summary of the invention
The technical problem that the present invention will solve is the deficiency that overcomes prior art; Providing a kind of can promote animal amino acid and trace copper to be carried out the feed addictive copper-threonine of synchronous absorption; And the preparation method of a kind of technology copper-threonine simple, with low cost is provided to this novel substance copper-threonine; Also provide a kind of copper-threonine to be applied to the method in the animal feed additive simultaneously, to reach the purpose of effective promotion growth of animal, enhancing animal resistance and raising efficiency of feed utilization.
For solving the problems of the technologies described above, the present invention proposes a kind of threonine chelated thing, it is characterized in that: the name of said threonine chelated thing is called copper-threonine, and its molecular formula is:
Copper-threonine of the present invention is blueness or powderblue powder, and is soluble in water, mainly absorbs through amino acid pinocytosis form in animal body.
The present invention also provides a kind of preparation method of above-mentioned copper-threonine, may further comprise the steps:
(1) with the crystalline hydrate of copper sulphate or copper sulphate and mass concentration is 5%～30% threonine aqueous solution; The crystalline hydrate of said copper sulphate or copper sulphate and the mol ratio of threonine are (0.5～1.1): 2; Under inert gas shielding, react; Reaction temperature is controlled at 60 ℃～100 ℃, adds NaOH and keeps the pH value 4～8 when reacting, and obtains product behind reaction 0.5～6h;
(2) said product is carried out vacuum and concentrates, perhaps obtain sediment with the moisture in the said product of organic solvent extraction until obtaining thick concentrate, more said concentrate or sediment are carried out drying, pulverizing after, obtain finished product copper-threonine.
Above-mentioned preparation method's main reaction formula is:
Among the above-mentioned preparation method, described inert gas is a nitrogen.
Among the above-mentioned preparation method, the vacuum degree control of said vacuum Concentrating Process is at 0.05～0.7kgf/cm
2, thickening temperature is controlled at 50 ℃～90 ℃, and concentration time is 0.5～4h.
Among the above-mentioned preparation method, said organic solvent is ethanol, propyl alcohol or butanols, and the volume ratio of the product of said organic solvent and its extraction is 4～12: 1, and the time of extraction is 2～30min.
Among the above-mentioned preparation method, the method for said drying is to adopt air-flow wink-dry method or flash distillation wink-dry method, and baking temperature is 80 ℃～160 ℃.
The present invention also provides the application of above-mentioned copper-threonine as animal feed additive.
With the additive of above-mentioned copper-threonine as the fattening pig feed; Add at feed per ton under the situation of 200～400g; Can obviously improve the speed of growth of fattening pig; Improve its price of deed, and help to improve fur outward appearance and the meat of fattening pig, and help trace elements absorbed utilizations such as iron, zinc.
With the additive of above-mentioned copper-threonine as sow feed; Add at feed per ton under the situation of 200～400g, can obviously improve the reproductive performance of sow and utilize the time limit, improve piglet birth weight and weight of weaning litter; Strengthen the resistance of piglet, improve the survival rate of piglet disease.
With the additive of above-mentioned copper-threonine as newborn pig starter feed, add at feed per ton under the situation of 200～400g, can improve newborn growth in piglets speed and survival rate, reduce the incidence of cad pig.
With the additive of above-mentioned copper-threonine as poultry feed, add at feed per ton under the situation of 100～200g, can promote the poultry growth, improve the utilization rate of poultry feed, strengthen the feather glossiness of poultry, improve the poultry anti-stress ability, reduce the death rate.
With the additive of above-mentioned copper-threonine as aquatic animal feed, add at feed per ton under the situation of 100～300g, can strengthen the immunity of aquatic livestock, reduce the disease incident of aquatic livestock.
With the additive of above-mentioned copper-threonine as ruminant feed; Add at feed per ton under the situation of 300～600g, can effectively improve the fur outward appearance of ruminant, keep the growth of its central nervous system; Improve the hemoglobin level of ruminant; Prevent exsanguine generation, improve milk crop, do not influence the content of milk composition.
The addition manner of above-mentioned feed addictive preferably adds with solid form, will make other composition in the feed can the oxidation because of the moisture absorption because add with solid form.
Compared with prior art, the invention has the advantages that: copper-threonine of the present invention is in fowl poultry alimentary canal, to absorb through the amino acid approach, and its infiltration rate is fast, and absorptivity improves greatly, and biological value is high, can reduce the consumption of feed addictive.Copper-threonine of the present invention also has good facilitation to the micro-element nutrition of improving animal.Copper-threonine of the present invention also can provide amino acid whose nutrition except that satisfying the needs of animal to trace copper, so can promote growth of animal, effectively avoid the destruction of inorganic copper to vitamin isoreactivity material in the feed, can significantly improve the utilization rate of feed.Copper-threonine of the present invention has stable chemical constitution as trace mineral supplement, safety non-toxic, and nonirritant, the eliminating amount of inorganic copper reduces in the animal wastes, therefore also can protect environment, reduces and pollutes.Trace mineral supplement copper-threonine of the present invention can be widely used in (like pig, fowl, aquatic livestock, ruminant etc.) in the various animal feeds.Can significantly improve the growth performance of animal, improve the utilization rate of feed.The preparation method who the present invention is directed to above-mentioned copper-threonine proposition also has advantages such as technology is simple, easy to operate, with low cost.
The specific embodiment
Embodiment 1:
A kind of finished product copper-threonine of the present invention is to prepare through following steps: at first open nitrogen valve, the air in the replacement reaction kettle; Under nitrogen protection, get the 40.55g threonine earlier then, put in the agitated reactor that fills the 100g reaction water and fully stir; Drop into the cupric sulfate pentahydrate of 42.5g again, the pH value of using 13.48g NaOH to keep reaction mixture under 90 ℃ of temperature is 6.8g, and reaction is 2.5 hours under the condition of normal pressure; Then product is delivered in the concentration tank, at 0.25kgf/c m
2Vacuum, 90 ℃ thickening temperature under, vacuum concentrates 45 minutes, treat product be thick after, with the concentrate blanking, go into dish, under 120 ℃ of temperature, carry out the air-flow wink-dry, prepare finished product copper-threonine after the pulverizing.
Adopt gel filtration chromatography (GB/T13080.2-2005) that the chelation percent in the above-mentioned product finished product is detected, it is worth all more than 80%.
The feeding experiment that the above-mentioned feed addictive that makes is used for weanling pig.Select the piglet that 90 ages in days are close, birth weight difference is little that feeding and management condition, reproductive effect is close and parity is identical 9 head length sows and the mating of same Du Luoke boar produced as test pig; Wean back is according to body weight, principle that sex ratio is identical; Be divided into control group and one, two group of test at random; Every group of 3 repetitions, each repeats 10 piglets.Wherein add copper sulphate 400g in the control group feed per ton, test one group of feed per ton and add copper-threonine 200g, two groups of feeds interpolation copper-threonine 400g per ton of test.Result of the test is seen table 1.
Table 1: copper-threonine is to the influence of weanling pig growth performance
Annotate: same column shoulder marking-up parent phase expression difference together is remarkable (p＞0.05) not; The female different expression significant difference (p＜0.05) of same column shoulder marking-up.
Can find out from table 1, on the total augment weight of duration of test test group and control group, average daily gain, feedstuff-meat ratio index, reach significant difference (p＜0.05), the test group differences is not significantly (p＞0.05) all; For survival rate and cad pig incidence, test group also shows the difference (p＜0.05) of conspicuousness than control group.This result of the test shows: in the weanling pig daily ration, add copper-threonine and can significantly promote growth in piglets speed and survival rate, reduce the generation of cad pig.
Implement row 2:
A kind of finished product copper-threonine of the present invention is to prepare through following steps: at first open nitrogen valve, the air in the replacement reaction kettle; Under nitrogen protection, get the 47.51g threonine earlier then, put in the agitated reactor that fills the 150g reaction water and fully stir; Drop into the 35.42g copper sulfate monohydrate again, the pH value of using 11.21g NaOH to keep reaction mixture under 90 ℃ of temperature is 6.8, reacts 2h under the condition of normal pressure; Then product is extracted 30min with propyl alcohol; The volume ratio of propyl alcohol and product is 10: 1, and the sediment after the extraction is carried out the flash distillation wink-dry under 120 ℃ of temperature, prepares finished product copper-threonine after the pulverizing.
Adopt gel filtration chromatography (GB/T13080.2-2005) that the chelation percent in the above-mentioned product finished product is detected, it is worth all more than 80%.
The feeding experiment that the above-mentioned feed addictive that makes is used for goose. select begin admittedly 162 of young geese of the health of the initial body weight basically identical of 23 ages in days for use, be divided into control group and one, two group of test immediately, every group of 3 repetitions, each repeats 18 geese.Wherein control group feed per ton adds copper sulphate 200g, tests one group of feed per ton and adds copper-threonine 100g, two groups of feeds interpolation copper-threonine 200g per ton of test.Result of the test is seen table 2.
Table 2: copper-threonine is to the influence of the young goose growth performance that begins admittedly
Annotate: same column shoulder marking-up parent phase expression difference together is remarkable (p＞0.05) not; The female expression significant difference (p＜0.05) inequality of same column shoulder marking-up.
Can find out from table 2, under the situation of just starting weight, feed consumption rate there are no significant influence (p＞0.05), control group and test group different Cu source and copper level to the weightening finish result of the white goose that begins admittedly growth period through multiple comparing difference all remarkable (p＜0.05).On material anharmonic ratio and death rate index, control group and test group also reach significant difference (p＜0.05), but test group differences not remarkable (p＞0.05).This result of the test shows: can significantly promote its growth, improve efficiency of feed utilization admittedly add copper-threonine in the white goose daily ration that begins; Improve resistance against diseases, reduce the death rate.
Execute example 3:
A kind of finished product copper-threonine of the present invention is to prepare through following steps: at first open nitrogen valve, the air in the replacement reaction kettle; Under nitrogen protection, get the 238Kg threonine earlier then, put in the agitated reactor that fills in the 1000Kg reaction water and fully stir; Drop into the 250Kg cupric sulfate pentahydrate again, the pH value of using 80Kg NaOH to keep reaction mixture under 90 ℃ of temperature is 6.5, reacts 2 hours under the condition of normal pressure; Then product is delivered in the concentration tank, at 0.5kgf/cm
2Vacuum, 80 ℃ temperature under, vacuum concentrates 30min, treat product be thick after, with the concentrate blanking, go into dish, under 120 ℃, carry out the air-flow wink-dry, prepare finished product copper-threonine after the pulverizing.
Adopt gel filtration chromatography (GB/T 13080.2-2005) that the chelation percent in the reactant finished product is detected, it is worth all more than 80%.
The feeding experiment that the above-mentioned feed addictive that makes is used for aquatic livestock.Select physique stalwartness, well-developed catfish 360 tails for use, be divided into 3 groups (two test group and a control group) immediately, every group of 3 repetitions, each repeats 40 tails; Test one group of feed per ton and add copper-threonine 100g, test two groups of feeds per ton and add copper-threonine 300g, control group feed per ton adds copper sulphate 300g.Result of the test is seen table 3.
Table 3: copper-threonine is to the influence of catfish growth performance
| Group | First counterpoise g | End counterpoise g | Tail daily gain g | Feed coefficient | Incidence of disease % |
| Control group | 39.38±0.89 a | 103.24±4.58 a | 1.57±1.24 a | 2.11±2.35 a | 11.25％ a |
| Test one group | 39.51±0.42 a | 139.17±6.27 b | 2.03±1.63 b | 1.81±2.47 b | 7.41％ b |
| Test two groups | 39.69±0.74 a | 157.36±6.52 b | 2.14±1.49 b | 1.67±3.04 b | 5.36％ b |
Annotate: same column shoulder marking-up parent phase expression difference together is remarkable (p＞0.05) not; The female expression significant difference (p＜0.05) inequality of same column shoulder marking-up.
Can find out from table 3; Each group is there was no significant difference (p＞0.05) between counterpoise just; But test group has the difference (p＜0.05) of conspicuousness than control group on last counterpoise, tail daily gain and feed coefficient index; But the test group differences is remarkable (p＞0.05) not, and the disease incident of test group significantly is lower than control group (p＜0.05).This result of the test shows: in the catfish diet, add growth, reduction feed coefficient and disease incident that copper-threonine can promote catfish.
The above-mentioned feed addictive that makes is used for the milk cow feeding experiment. select 18 to be in the lactation healthy china holstein cows in early stage,, be divided into 3 groups (two test group and a control group) at random, 6 every group; Test one group of feed per ton and add copper-threonine 300g, test two groups of feeds per ton and add copper-threonine 600g, control group feed per ton adds copper sulphate 600g.Result of the test is seen table 4.
Table 4: copper-threonine is to the influence of milk cow production performance
| Group | Daily yielding kg | Milk sugar rate % | Butterfat percnetage % | Protein ratio % | Milk dry matter rate % |
| Control group | 14.21 a | 4.66±0.05 a | 3.59±0.19 a | 3.26±0.15 a | 11.53±0.38 a |
| Test one group | 15.89 b | 4.59±0.21 a | 3.65±0.29 a | 3.21±0.21 a | 11.76±0.32 a |
| Test two groups | 15.97 b | 4.49±0.19 a | 3.67±0.20 a | 3.24±0.13 a | 11.96±0.39 a |
Annotate: same column shoulder marking-up parent phase expression difference together is remarkable (p＞0.05) not; The female expression significant difference (p＜0.05) inequality of same column shoulder marking-up.
Can find out that from table 4 average daily milk production of test group is 15.93kg, control group is 14.21kg, and daily yielding improves 12.10%, significant difference (p＜0.05), but test group differences not significantly (p＞0.05); There was no significant difference (p＞0.05) between each is organized on milk sugar rate, butterfat percnetage, protein ratio, the milk dry matter rate index.This result of the test shows: in dairy cow diet, add copper-threonine and can significantly improve the output of milk, but the milk composition is not had influence.
Claims (5)
1. a name is called the application of the threonine chelated thing of copper-threonine, and it is characterized in that: said copper-threonine is the additive that is used to prepare animal feed, and its molecular formula is:
2. the application of copper-threonine according to claim 1 is characterized in that: said copper-threonine is the feed addictive that is used to prepare pig feed, and the addition of said feed addictive is that pig feed per ton adds 200～400g.
3. the application of copper-threonine according to claim 1 is characterized in that: said copper-threonine is the feed addictive that is used to prepare poultry feed, and the addition of said feed addictive is that poultry feed per ton adds 100～200g.
4. the application of copper-threonine according to claim 1 is characterized in that: said copper-threonine is the feed addictive that is used to prepare aquatic livestock, and the addition of said feed addictive is that aquatic animal feed per ton adds 100～300g.
5. the application of copper-threonine according to claim 1 is characterized in that: said copper-threonine is the feed addictive that is used to prepare ruminant, and the addition of said feed addictive is that ruminant feed per ton adds 300～600g.
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| CN2011101956926A CN102334604A (en) | 2009-05-05 | 2009-05-05 | Application of copper threonate |
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| CN2011101956926A CN102334604A (en) | 2009-05-05 | 2009-05-05 | Application of copper threonate |
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| CN2009100432998A Division CN101597235B (en) | 2009-05-05 | 2009-05-05 | Copper-threonine and preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102550830A (en) * | 2012-02-17 | 2012-07-11 | 长沙兴嘉动物营养科技有限公司 | Method for applying and preparing gamma-aminobutyric acid complex compound |
| CN104761462A (en) * | 2015-03-23 | 2015-07-08 | 长沙兴嘉生物工程股份有限公司 | A preparing method of a threonine copper compound |
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- 2009-05-05 CN CN2011101956926A patent/CN102334604A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1103640A (en) * | 1993-12-09 | 1995-06-14 | 深圳东深科技发展有限公司 | Chemosynthesis process for threonine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550830A (en) * | 2012-02-17 | 2012-07-11 | 长沙兴嘉动物营养科技有限公司 | Method for applying and preparing gamma-aminobutyric acid complex compound |
| CN104761462A (en) * | 2015-03-23 | 2015-07-08 | 长沙兴嘉生物工程股份有限公司 | A preparing method of a threonine copper compound |
| CN104761462B (en) * | 2015-03-23 | 2017-03-15 | 长沙兴嘉生物工程股份有限公司 | A kind of preparation method of copper-threonine |
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Application publication date: 20120201 |