CN101692825B - Pesticide composition containing carbosulfan and application thereof - Google Patents

Abstract

The invention relates to a pesticide composition, wherein effective components comprise carbosulfan and flonicamid. The pesticide composition has the obvious synergy effect on preventing and controlling pests in agriculture, particularly on resistant rice planthopper and thrips. By using in the field, the invention can reduce the pesticide dosage as well as the environmental pollution and pesticide residues and delay the development speed of the pesticide resistance of pests.

Description

A kind of Pesticidal combination and application thereof that contains carbosulfan

Technical field

The present invention relates to a kind of composition pesticide and application thereof.

Background technology

In agricultural production, breeding amount is big, the tangible insect of generation overlap, and is very fast like the resistance generation speed of insects such as plant hopper, aphid, aleyrodid, thrips, and the speed of exploitation new varieties of pesticides does not catch up with the resistance development speed of insect far away.So, the composite of agricultural chemicals just seems very valuable, particularly the mechanism of action different, each other the composite of the medicament of coordinating synergistic effect arranged, can improve drug effect, and the development of delaying drug resistance, to prolong the service life of effective ingredient.

Rice hoppers is one type of important homoptera pest, is migratory pest common on the paddy rice, and the rice clump is agglomerating withered when serious, even the dead stalk lodging in full field.According to the result of rice fulgorid resistance monitoring, along with constantly migrating, the resistance of rice fulgorid constantly rises, and this has brought great difficulty for the control of rice fulgorid.Medicaments such as Imidacloprid commonly used in the past, Mobucin use very poor to the rice fulgorid control efficiency separately, especially to brown planthopper and small brown rice planthopper.

Thrips is important thrips insect, is common in melon bean kind vegetable and fruit tree.It is very big to have breeding amount, and the bodily form is little, is prone to characteristics such as outburst.The tender flower of main harm, the tender tip and fruitlet, cause use up the fruit, plant stunts and the melon and fruit exterior quality under degradation, can cause heavy losses, percent of loss can reach 10%-50%.In recent years in Hainan, the melon and fruit growing area in Guangdong rises to primary pest.Use such as main control medicament such as Imidacloprid, carbosulfan etc. separately cannot be prevented and treated this insect.

Carbosulfan, english common name carbosulfan, chemical name 2,3-dihydro-2,2-dimethyl benzofuran-7-base (dibutylamino sulphur) methyl carbamate.Carbosulfan belongs to carbamate insecticide, the inhibitor of cholinesterase, have tag, stomach toxicity, interior absorption, effect is fast rapidly, especially Homoptera, thrips insect is had good preventive effect.But owing to use for many years, a lot of insects produce resistance to it, thereby cause control efficiency obviously to descend.

Flonicamid, English common name flonicamid is a kind of picolinamide class insect growth regulator, IGR, is mainly used on cotton, cucurbit, fruit, vegetables, potato, the operatic circle and the drupe.Its mechanism of action is unique, and insect is had good effects on neural system and quick antifeedant activity, does not have cross resistance with existing insecticide.But its pesticide resistance risk of using separately is also higher.

Summary of the invention

The purpose of this invention is to provide a kind of Pesticidal combination.Said composition synergy is remarkable, and the control spectrum is wide, and the antagonism pest controling effect is good, and is particularly evident to the control that produces resistance rice planthopper and thrips; Another purpose of invention provides the application of said composition in control of insect.

For solving the problems of the technologies described above; The inventor is through a large amount of biologicall test screenings; Find that carbosulfan and flonicamid are composite with certain proportion; Antagonism rice fulgorid and thrips have obvious synergistic effect, to other insects, like homoptera pests such as trialeurodes vaporariorum, aphid, Bemisia tabaci, leafhopper good field control effect are arranged also.

Technical scheme of the present invention is:

The insecticide of employing different effects mechanism carries out the biological activity determination of different proportionings, occurs three kinds of condition of different after the determination of activity of different proportionings usually: summation action, antagonism, synergistic effect.With the synergy proportioning is that effective ingredient is mixed with suitable preparation, is used to prevent and treat the insect on the crops, can effectively reduce the usage amount of medicament, reduces drug cost, the generation of delaying drug resistance, thus reduce pollution to environment.

The present invention adopts carbosulfan and flonicamid to prepare Pesticidal combination, and the mass ratio of flonicamid and carbosulfan is 1: 1～1: 160, and preferred ratio is 1: 10～1: 40.Said carbosulfan and the flonicamid gross mass content in preparation is 1%-85%, preferred 5%-50%.

The present composition is processed into any dosage formulation of using on the suitable agricultural in practical application, preferred dosage form is missible oil, microemulsion, aqueous emulsion, water dispersible granules, wetting powder, suspending agent.

Present composition preparation can be used for preventing and treating the insect of harm plant, is particularly useful for preventing and treating the insect that existing medicament has been produced resistance on the plant, more specifically is applicable to and prevents and treats resistance plant hopper and thrips.

Composition pesticide of the present invention compared with prior art produces following beneficial effect: two kinds of active ingredients in (1) composition have synergistic effect, and the synergy of antagonism insect is particularly evident, compare with single agent, have obviously improved control efficiency; (2) composition combines the effect characteristics of two kinds of effective ingredients, compares with single agent to have enlarged the control spectrum; (3) can reduce the field dosage, effectively reduce environmental pollution and residue of pesticide, reduce harm beneficial organism; (4) mechanism of action of both active ingredients is different in the composition, and the application of composition helps to delay pest resistance development, prolongs medicament service life.

Embodiment

In order to make the object of the invention, technical scheme and technological merit are clearer, below in conjunction with embodiment summary of the invention are done further to specify.Should be appreciated that described specific embodiment only is used to explain the present invention, and be not used in qualification the present invention.All any modifications of within spirit of the present invention and principle, being made, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.

Embodiment one: carbosulfan and flonicamid are to rice fulgorid, the test of thrips indoor virulence

It is tested object that paddy rice brown planthopper and thrips are adopted in test, and concrete grammar is:

(1) test medicine:

The former medicine of 90% carbosulfan (Hunan Haili Chemical Co., Ltd.);

The former medicine of 97.6% flonicamid (SUMITOMO CHEMICAL Co., Ltd.);

Former medicine is mixed with the test medicine that needs by conventional method.

(2) subjects:

Paddy rice brown planthopper (Nilaparvata lugens (Stal)): the brown planthopper nymph in 3 age that indoor paddy rice seedling is raised.Responsive examination worm is from Agricultural University Of Nanjing, and resistance examination worm is picked up from paddy rice base, Lingshui, Hainan county.

Flower thrips (Frankliniella intonsa): the thrips adult that indoor bean seedlings are raised.Responsive examination worm is picked up from beautiful rule food market, Shenzhen, and resistance examination worm is picked up from margin cities and towns, Sanya, Hainan.

(3) test method:

Brown planthopper: test method is with reference to " the agricultural industry criteria NY/T1154.11-2008 of the People's Republic of China (PRC) "; Adopt rice stem infusion process: extract with root the rice strain at healthy and strong tillering stage to booting initial stage, clean, be cut into the long band root rice stem of 15cm; One group of 3 strain locate to dry in the air not have washmarking to the surface in the cool place.The rice stem was soaked 30 seconds in the good medicament of dilution respectively; After taking-up is dried, encase root with the absorbent cotton that soaks and put into and cultivate cup, draw 3 mid-term in age nymph put into and cultivate cup; 20 every glass; Put temperature and be 27 ℃, photoperiod and be 16h: 8h (L: raise in incubator D), the clear water contrast, each concentration repeats 3 times.Check death toll after 4 days.

Thrips: test method is with reference to " the agricultural industry criteria NY/T1154.7-2006 of the People's Republic of China (PRC) ".Test method adopts leaf dipping method.Adopt the beans leaf dish of diameter 1cm to soak soup 5 seconds, put into dactylethrae after taking-up is dried.Clear water is soaked in contrast.Every dactylethrae inserts 20 thrips adults.Fruit came to an end after 72 hours.Check result, data and virulence evaluation method are identical with the test of rice fulgorid virulence.

(4) statistical analysis

Carry out statistical analysis with the DPS data processing software, calculate the LC of each medicament ₅₀And 95% confidence limit, estimate with this and to supply the insecticidal activity of reagent agent the examination worm.

The co-toxicity coefficient of mixture (CTC value) calculates by following formula:

$\mathrm{ATI}=\frac{S}{M}\×100$

In the formula:

ATI---mixture actual measurement toxicity index;

The LC of S---standard insecticide ₅₀, unit is mg/l;

The LC of M---mixture ₅₀, unit is mg/l.

TTI＝TI _AxP _A+TI _BxP _B

In the formula:

The theoretical toxicity index of TTI---mixture;

TI _A----A medicament toxicity index;

P _AThe percentage composition of-----A medicament in mixture, unit is a percentage;

TI _B----B medicament toxicity index;

P _BThe percentage composition of-----B medicament in mixture, unit is a percentage.

$\mathrm{CTC}=\frac{\mathrm{ATI}}{\mathrm{TTI}}\×100$

In the formula:

The CTC----co-toxicity coefficient;

ATI---mixture actual measurement toxicity index;

The theoretical toxicity index of TTI---mixture.

Composite dose co-toxicity coefficient (CTC)>=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.

(5) interpretation of result

1, different disposal is to the toxicity test result of brown planthopper:

Resistance and responsive brown planthopper virulence test result see the following form:

Table 1 different disposal is to toxicity test result's (sensitive population) of responsive brown planthopper

Handle	LC 50(mg/ L)	AT1	TTI	Co-toxicity coefficient CTC
					Flonicamid	1.89	100.00
Carbosulfan	12.01	15.74
					Flonicamid 1+ carbosulfan 1	2.32	81.47	57.87	140.78
Flonicamid 1+ carbosulfan 5	4.45	42.47	29.78	142.62
					Flonicamid 1+ carbosulfan 10	4.67	40.47	23.40	172.97
Flonicamid 1+ carbosulfan 20	5.64	33.51	19.75	169.68
					Flonicamid 1+ carbosulfan 40	8.70	21.72	17.79	122.10
Flonicamid 1+ carbosulfan 80	9.12	20.72	16.78	123.52
					Flonicamid 1+ carbosulfan 160	9.45	20.00	16.26	123.00

Toxicity test result's (resistant population) of table 2 different disposal antagonism brown planthopper

Handle	LC 50 (mg/L)	AT1	TTI	Co-toxicity coefficient CTC	Resistance index
						Flonicamid	2.42	100.00			1.28
Carbosulfan	434.23	0.56			36.16
						Flonicamid 1+ carbosulfan 1	2.34	103.42	50.28	205.69
Flonicamid 1+ carbosulfan 5	6.34	38.17	17.13	222.81
						Flonicamid 1+ carbosulfan 10	7.63	31.72	9.60	330.47
Flonicamid 1+ carbosulfan 20	12.12	19.97	5.29	377.26
						Flonicamid 1+ carbosulfan 40	21.67	11.17	2.98	374.40
Flonicamid 1+ carbosulfan 80	49.35	4.90	1.78	274.72
						Flonicamid 1+ carbosulfan 160	89.42	2.71	1.17	230.33

Find out that from table 1,2 flonicamid and carbosulfan are composite, mass ratio is 1: 1-1:, the CTC of sensitive population and resistant population brown planthopper all greater than 120, is had synergistic effect at 160 o'clock.Wherein antagonism population (to the carbosulfan resistance index up to 36.16) CTC is higher than sensitive population all greater than 200, shows that the synergy of the composite antagonism population of flonicamid and carbosulfan is particularly evident.In the resistant population test, be 1 with flonicamid and carbosulfan mass ratio again: 10-1: CTC was obviously higher in 40 o'clock, explained that synergy is more obvious under this proportioning.

2, different disposal is to the toxicity test result of thrips:

Resistance and responsive flower thrips virulence test result see the following form:

Table 3 different disposal is to toxicity test result's (sensitive population) of responsive flower thrips

Handle	LC 50(mg/ L)	AT1	TTI	Co-toxicity coefficient CTC
					Flonicamid	0.78	100.00
Carbosulfan	11.45	6.81
					Flonicamid 1+ carbosulfan 1	1.11	70.27	53.41	131.58
Flonicamid 1+ carbosulfan 5	2.56	30.47	22.34	136.37
					Flonicamid 1+ carbosulfan 10	3.13	24.92	15.28	163.05
Flonicamid 1+ carbosulfan 20	4.51	17.29	11.25	153.74
					Flonicamid 1+ carbosulfan 40	5.67	13.76	9.09	151.42
Flonicamid 1+ carbosulfan 80	7.76	10.05	7.96	126.23
					Flonicamid 1+ carbosulfan 160	7.80	10.00	7.39	135.30

Toxicity test result's (resistant population) of table 4 different disposal antagonism flower thrips

Handle	LC 50 (mg/L)	AT1	TTI	Co-toxicity coefficient CTC	Resistance index
						Flonicamid	0.79	100.00			1.01
Carbosulfan	806.65	0.10			70.45
						Flonicamid 1+ carbosulfan 1	0.69	114.49	50.05	228.76
Flonicamid 1+ carbosulfan 5	2.20	35.91	16.75	214.40
						Flonicamid 1+ carbosulfan 10	2.56	30.86	9.18	336.16
Flonicamid 1+ carbosulfan 20	4.66	16.95	4.86	349.17
						Flonicamid 1+ carbosulfan 40	8.75	9.03	2.53	356.22
Flonicamid 1+ carbosulfan 80	22.89	3.45	1.33	259.24
						Flonicamid 1+ carbosulfan 160	45.67	1.73	0.72	240.77

Find out that from table 3,4 flonicamid and carbosulfan are composite, mass ratio is 1: 1-1:, the CTC of sensitive population and resistant population flower thrips all greater than 120, is had synergistic effect at 160 o'clock.Wherein antagonism population (to the carbosulfan resistance index up to 70.45) CTC is higher than sensitive population all greater than 200, shows that the synergy of the composite antagonism population of flonicamid and carbosulfan is particularly evident.In the resistant population test, be 1 with flonicamid and carbosulfan mass ratio again: 10-1: CTC was obviously higher in 40 o'clock, explained that synergy is more obvious under this proportioning.

Embodiment three: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 2.78 grams of 90% carbosulfan, former medicine 2.60 grams of 97.6% flonicamid, calcium dodecyl benzene sulfonate 5 grams, OPEO 4 restrain, supply gross weight to 100 gram with xylol.With mixing in the method input mixing kettle of above component by routine preparation missible oil, stirring forms preparation 5% flonicamid carbosulfan missible oil (mass ratio of flonicamid and carbosulfan is 1: 1).

Should fill a prescription and dilute spray, be used to prevent and treat rice hoppers (brown planthopper accounts for more than 95%, and the resistance of Imidacloprid more than 160 times, is belonged to high resistance) by effective ingredient 30 gram/hectares.Behind the medicine 1 day control efficiency be 98.31%, 20% carbosulfan missible oil and 10% flonicamid missible oil according to using with quadrat method and dosage, 1 day preventive effect is respectively 46.12% and 81.27%, significant difference behind the medicine.

7 days control efficiency are that 7 days preventive effects are respectively 15.12% and 75.27%, significant difference behind 88.31%, 20% carbosulfan missible oil and the 10% flonicamid missible oil medicine behind the present embodiment product medicine.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, thereby reduce environmental pollution, reduce residue of pesticide.

Embodiment four: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 27.78 grams of 90% carbosulfan, former medicine 2.60 grams of 97.6% flonicamid, calcium dodecyl benzene sulfonate 13.5 grams, dimethyl formamide 8.9 grams, propane diols 10.2 gram waters are supplied gross weight to 100 gram.Carbosulfan and the former medicine of flonicamid are dissolved with dimethyl formamide earlier; Ratio in prescription adds propane diols, calcium dodecyl benzene sulfonate mixes; Under agitation mixture is added in the entry, obtain 27.5% flonicamid carbosulfan microemulsion (mass ratio of flonicamid and carbosulfan is 1: 10) after being stirred to evenly.

Should fill a prescription by effective ingredient 20 gram/hectares dilution spraying, be used for EC against Citrus Aphides.Behind the medicine 1 day control efficiency be 99.31%, 20% carbosulfan microemulsion and 5% flonicamid microemulsion according to using with quadrat method and dosage, 1 day preventive effect is respectively 54.33% and 75.17%, significant difference behind the medicine.Inspection simultaneously finds that also this compound drug also has certain effect to the citrus rust mite, and preventive effect is 95.34%, and the agent of flonicamid list is invalid to rust mite.

The present embodiment product was that 90.34%, 20% carbosulfan microemulsion and 5% flonicamid microemulsion are respectively 27.12% and 71.27%, significant difference according to what use with quadrat method and dosage to the citrus aphid control efficiency in 7 days behind the medicine.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, the expanding prevention spectrum, thus reduce environmental pollution, reduce residue of pesticide.

Embodiment five: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 44.44 grams of 90% carbosulfan, the former medicine 1.02 of 97.6% flonicamid restrains, alkylaryl polyoxyethylene polyoxypropylene ether 6.9 restrains, gathers naphthaldehyde sulfonate sodium 3.5 grams, xanthans 0.89 restrains, water is supplied gross weight to 100 gram.Above component is mixed, after high speed shear, obtain 41% flonicamid carbosulfan suspending agent (mass ratio of flonicamid and carbosulfan is 1: 40).

Should fill a prescription by effective ingredient 30 gram/hectares dilution spraying, be used to prevent and treat the cucumber thrips.Behind the medicine 1 day control efficiency be 97.35%, 20% carbosulfan suspending agent and 5% flonicamid suspending agent according to using with quadrat method and dosage, 1 day preventive effect is respectively 77.33% and 78.17%, significant difference behind the medicine.Inspection simultaneously finds that also this compound drug also has certain effect to the cucumber aleyrodid, and preventive effect is 89.33%, and the agent of carbosulfan list is invalid basically to aleyrodid.

The present embodiment product to cucumber thrips medicine after 7 days control efficiency be that 89.78%, 20% carbosulfan suspending agent and 5% flonicamid suspending agent are respectively 46.17% and 72.56%, significant difference according to what use with quadrat method and dosage.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, the expanding prevention spectrum, thus reduce environmental pollution, reduce residue of pesticide.

Embodiment six: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 22.22 grams of 90% carbosulfan, former medicine 1.02 grams of 97.6% flonicamid, calcium dodecyl benzene sulfonate 5.5 grams, N-Methyl pyrrolidone 10.5 grams, dimethyl formamide 5.8 restrain, supply gross weight to 100 gram with deionized water.The former medicine of carbosulfan and flonicamid, dimethyl formamide, calcium dodecyl benzene sulfonate added with N-Methyl pyrrolidone be in the same place, make dissolving, be stirred into oil phase; Under high-speed stirred, oil phase is mixed with water, make 21% flonicamid carbosulfan aqueous emulsion (mass ratio of flonicamid and carbosulfan is 1: 20).

Should fill a prescription by effective ingredient 30 gram/hectares dilution spraying, be used for control joint melon thrips.Behind the medicine 1 day control efficiency be 95.31%, 20% carbosulfan aqueous emulsion and 5% flonicamid aqueous emulsion according to using with quadrat method and dosage, 1 day preventive effect is respectively 55.77% and 76.12%, significant difference behind the medicine.Inspection simultaneously finds that also this compound drug also has certain effect to joint melon aphid, and preventive effect is 89.33%.

The present embodiment product to joint melon thrips medicine after 7 days control efficiency be that 85.44%, 20% carbosulfan aqueous emulsion and 5% flonicamid aqueous emulsion are respectively 42.14% and 69.86%, significant difference according to what use with quadrat method and dosage.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, the expanding prevention spectrum, thus reduce environmental pollution, reduce residue of pesticide.

Embodiment six: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 88.89 grams of 90% carbosulfan; Former medicine 0.52 gram of 97.6% flonicamid, sodium lignin sulfonate 1.1 grams, PO-EO block polyether 2.1 restrain, gather naphthaldehyde sulfonate sodium 2.4 grams, ammonium sulfate 3.6 grams, supply gross weight to 100 gram with precipitated calcium carbonate; Above component is mixed; Become wetting powder through comminution by gas stream, add the granulation of a certain amount of water extrusion again.After dry screened, make 80.5% flonicamid carbosulfan water-dispersible granules (mass ratio of flonicamid and carbosulfan is 1: 160).

Should fill a prescription by effective ingredient 120 gram/hectares dilution spraying, be used to prevent and treat the plastic tent cucumber Bemisia tabaci.Behind the medicine 1 day control efficiency be 93.67%, 50% carbosulfan water-dispersible granules and 50% flonicamid water-dispersible granules according to using with quadrat method and dosage, 1 day preventive effect is respectively 46.77% and 89.13%, significant difference behind the medicine.Inspection simultaneously finds that also this compound drug also has certain effect to the cucumber liriomyza bryoniae, and preventive effect is 85.33%.

The present embodiment product to cucumber Bemisia tabaci medicine after 7 days control efficiency be 82.64%; 50% carbosulfan water-dispersible granules and 50% flonicamid water-dispersible granules are respectively 23.17% and 70.84%, significant difference according to what use with quadrat method and dosage.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, the expanding prevention spectrum, thus reduce environmental pollution, reduce residue of pesticide.

Embodiment seven: composite dose of preparation of carbosulfan and flonicamid and field efficacy

Take by weighing former medicine 22.22 grams of 90% carbosulfan, former medicine 0.52 gram of 97.6% flonicamid, lauryl sodium sulfate 2.4 grams, sodium lignin sulfonate 5.5 grams, naphthalene sulfonate 3.6 restrain, complement to 100 grams with kaolin., above component is mixed, become wetting powder to make 20.5% flonicamid carbosulfan wetting powder (mass ratio of flonicamid and carbosulfan is 1: 40) through comminution by gas stream.

Should fill a prescription by effective ingredient 90 gram/hectares dilution spraying, be used to prevent and treat rice hoppers (white-backed planthopper accounts for about 90%).Behind the medicine 1 day control efficiency be 96.65%, 50% carbosulfan water-wettable pulvis and 50% flonicamid wetting powder according to using with quadrat method and dosage, 1 day preventive effect is respectively 36.37% and 83.53%, significant difference behind the medicine.Inspection simultaneously finds that also this compound drug also has certain effect to rice-leaf hopper, and preventive effect is 82.33%.

The present embodiment product to the rice fulgorid medicine after 7 days control efficiency be that 89.84%, 50% carbosulfan wetting powder and 50% flonicamid wetting powder are respectively 7.87% and 79.14%, significant difference according to the preventive effect of using with quadrat method and dosage.Show that composite dose of lasting period rise appreciably, can reduce the medication number of times, the expanding prevention spectrum, thus reduce environmental pollution, reduce residue of pesticide.

Claims (5)

1. composition pesticide, it is characterized in that: effective ingredient is carbosulfan and flonicamid, the mass ratio of flonicamid and carbosulfan is 1: 1-1: 160.

2. composition pesticide according to claim 1 is characterized in that: the mass ratio of effective ingredient flonicamid and carbosulfan is 1: 10-1: 40.

3. composition pesticide according to claim 1 and 2 is characterized in that: effective ingredient carbosulfan and the flonicamid quality percentage composition in composition pesticide is 1%～85%.

4. according to the composition pesticide described in the claim 3, it is characterized in that: be mixed with one or more formulations in missible oil, microemulsion, aqueous emulsion, water dispersible granules, wetting powder and the suspending agent.

5. according to claim 1 or claim 2 the application of composition pesticide on control resistant rice plant hopper and thrips.