Summary of the invention
The purpose of this invention is to provide a kind of feed addictive of layer chicken and using method take hypericum perforatum as primary raw material, be intended to improve performance in layers, reduce egg lipid material content, improve the antibiotic antioxygenic property of egg, prevent and treat simultaneously the poultry bird flu.Satisfy breeding layer chicken industry to the demand of natural green, with low cost, multifunction quick-effect feed addictive.
The technical solution adopted in the present invention is finished in the following way: a kind of feed addictive of layer chicken, the component of this feed addictive of layer chicken comprises Hypericum Perforatum P.E, phytase and vitamin C, and the weight proportion of the component of feed addictive of layer chicken is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs.
In order to strengthen the stable and easy to use of additive, can add carrier in above-mentioned feed addictive of layer chicken makes it become the dilution type feed addictive of layer chicken, the weight proportion of the component of dilution type feed addictive of layer chicken is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs, 400~600 parts in carrier, wherein, carrier adopts a kind of in white carbon, diatomite, the zeolite powder, optimization be to adopt white carbon as carrier.
The preparation method of feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, after the harvesting lucifuge and with 35 ℃~55 ℃ temperature clear water clean, immersion 2~4 hours;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding and extract solvent, then under 20 ℃~40 ℃, extracted 20~40 minutes with ultrasonic wave; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
The preparation of feed addictive of layer chicken is divided into the preparation of plain edition feed addictive of layer chicken and the preparation of dilution type feed addictive of layer chicken, wherein:
(1) preparation of plain edition feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase and vitamin C, stir, after packing a kind of plain edition feed addictive of layer chicken.
(2) preparation of dilution type feed addictive of layer chicken
Weight proportion by dilution type feed addictive of layer chicken component takes by weighing Hypericum Perforatum P.E, phytase, vitamin C and carrier, wherein, the vegetable oil that adds vehicle weight percentage 0.5~1% in carrier, vegetable oil adopt a kind of in soya-bean oil, corn oil, peanut oil, cottonseed oil, the rapeseed oil; During preparation, in carrier, add first vegetable oil, to be mixed evenly after, add successively Hypericum Perforatum P.E, vitamin C and phytase, after mixing, packing a kind of dilution type feed addictive of layer chicken.
In the preparation method of above-mentioned feed addictive of layer chicken, the used hypericum perforatum plant of preparation Hypericum Perforatum P.E is to adopt the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant.
In the preparation method of above-mentioned feed addictive of layer chicken, in the preparation process of Hypericum Perforatum P.E (4), it is used that to dissolve chlorophyllous solvent be a kind of in chloroform, the ether, optimization be to adopt chloroform as dissolving chlorophyllous solvent; As can dissolve chlorophyllous solvent the time, the proportioning of described hypericum perforatum powder and chloroform is: described hypericum perforatum powder 1kg, chloroform 3000ml~8000ml with chloroform.
In the preparation method of above-mentioned feed addictive of layer chicken, in the preparation process of Hypericum Perforatum P.E (5), used extraction solvent is a kind of in methyl alcohol, ethanol, normal propyl alcohol, acetone, the ethyl acetate, optimization be to adopt methyl alcohol as solvent; When extracting solvent, the filtered residue of described drying and the proportioning of methyl alcohol are: the filtered residue 1kg of described drying, methyl alcohol 3000ml~8000ml with methyl alcohol.
The using method of feed addictive of layer chicken of the present invention is:
In the egg feedstuff of 10kg, add plain edition feed addictive of layer chicken 5~10g of the present invention and mix, the operating process lucifuge of trying one's best.In order to keep the effect of additive,<15 days batch mixings once and pack feed addictive, place shady and cool drying and lucifuge place.
Or in the egg feedstuff of 10kg, add dilution type feed addictive of layer chicken 20~50g of the present invention and mix, the operating process lucifuge of trying one's best.In order to keep the effect of additive,<15 days batch mixings once and pack feed addictive, place shady and cool drying and lucifuge place.
Giving the laying hen feeding time is respectively to feed once in the morning and afternoon of every day, and the laying hen feeding time of giving of optimization is that 8 of every days and 15 respectively feed once.
Plain edition feed addictive of layer chicken of the present invention can add in the drinking-water of laying hen, and additional proportion is 500mg/kg~1000mg/kg.
Phytase in above-mentioned feed addictive of layer chicken component has the digestibility that can improve the elements such as calcium, phosphorus in the laying hen plant feed, alleviates hypericin and reduces the effect that blood calcium causes the egg shell thickness to reduce.
Vitamin C in above-mentioned feed addictive of layer chicken component helps in the laying hen body cholesterol to resolve into cholic acid to excrete, strengthen laying hen body disease-resistant power and defensive enginery, the vitamin C in the feed can prevent that active component is oxidized in the Hypericum Perforatum P.E simultaneously.
Find through experiment: adopt feed addictive of layer chicken of the present invention to feed after 4 weeks, compare with control group (not adding feed addictive of layer chicken of the present invention), each index result is as follows: egg size increase by 2.76%, feedstuff-egg ratio reduce by 3.98%, the egg volume improves 3.80%, and the laying rate of laying hen is without impact; Yolk SOD content improves 7.43%, and yolk MDA reduces by 35.91%; Laying-hen Serum and yolk total cholesterol level reduce respectively 27.92% and 13.79%, yolk triglyceride content 16.23%; Egg white lysozyme content improves 11.18%; The laying hen quantity of leucocyte improves 41.84%.Therefore feed addictive of the present invention can improve performance in layers, reduced the breeding production cost, improved the egg oxidation stability, reduced the lipid material content of egg, make egg effectively resist bacterial invasion, strengthen the egg property stability, prolong the egg product period of storage, and improved immunity of layer chicken.
The specific embodiment
Below by embodiment the present invention is made further and to specify, but the invention is not restricted to these embodiment.
Embodiment 1
A kind of feed addictive of layer chicken, the component of this additive comprises Hypericum Perforatum P.E, phytase and vitamin C, the weight proportion of the component of additive is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs.
The preparation method of this feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, lucifuge is also cleaned, was soaked 2~4 hours with 35 ℃~55 ℃ temperature clear water after the harvesting, wherein, the hypericum perforatum plant is adopted the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding methyl alcohol, then extracted 20~40 minutes with ultrasonic wave under 20 ℃~40 ℃, dry filtered residue and the proportioning of methyl alcohol are: dry filtered residue 1kg, methyl alcohol 3000ml~8000ml; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase and vitamin C, stir, after packing a kind of feed addictive of layer chicken.
The using method of feed addictive of layer chicken of the present invention is:
In the egg feedstuff of 10kg, add plain edition feed addictive of layer chicken 5~10g of the present invention and mix, the operating process lucifuge of trying one's best.In order to keep the effect of additive,<15 days batch mixings once and pack feed addictive, place shady and cool drying and lucifuge place.
Giving the laying hen feeding time is respectively to feed once in the morning and afternoon of every day, and the laying hen feeding time of giving of optimization is that 8 and 15 of every day respectively feed once.
Feed addictive of layer chicken of the present invention is added in the drinking-water of laying hen, additional proportion is 500mg/kg~1000mg/kg.
Embodiment 2
A kind of feed addictive of layer chicken, the component of this additive comprises Hypericum Perforatum P.E, phytase, vitamin C and white carbon, and the weight proportion of the component of additive is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs, 400~600 parts of white carbons.
The preparation method of this feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, lucifuge is also cleaned, was soaked 2~4 hours with 35 ℃~55 ℃ temperature clear water after the harvesting, wherein, the hypericum perforatum plant is adopted the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding ethanol, then extracted 20~40 minutes with ultrasonic wave under 20 ℃~40 ℃, dry filtered residue and the proportioning of ethanol are: dry filtered residue 1kg, ethanol 3000ml~8000ml; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase, vitamin C and white carbon, wherein, the vegetable oil that adds vehicle weight percentage 0.5~1% in white carbon, vegetable oil adopt a kind of in soya-bean oil, corn oil, peanut oil, cottonseed oil, the rapeseed oil; During preparation, in white carbon, add first vegetable oil, to be mixed evenly after, add successively Hypericum Perforatum P.E, vitamin C and phytase, after mixing, packing a kind of dilution type feed addictive of layer chicken.
The using method of dilution type feed addictive of layer chicken of the present invention is:
In the egg feedstuff of 10kg, add dilution type feed addictive of layer chicken 20~50g of the present invention and mix, the operating process lucifuge of trying one's best.In order to keep the effect of additive,<15 days batch mixings once and pack feed addictive, place shady and cool drying and lucifuge place.
Giving the laying hen feeding time is respectively to feed once in the morning and afternoon of every day, and the laying hen feeding time of giving of optimization is that 8 and 15 of every day respectively feed once.
Embodiment 3
A kind of feed addictive of layer chicken, the component of this additive comprises Hypericum Perforatum P.E, phytase, vitamin C and diatomite, and the weight proportion of the component of additive is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs, 400~600 parts in diatomite.
The preparation method of this feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, lucifuge is also cleaned, was soaked 2~4 hours with 35 ℃~55 ℃ temperature clear water after the harvesting, wherein, the hypericum perforatum plant is adopted the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding normal propyl alcohol, then extracted 20~40 minutes with ultrasonic wave under 20 ℃~40 ℃, dry filtered residue and the proportioning of normal propyl alcohol are: dry filtered residue 1kg, normal propyl alcohol 3000ml~8000ml; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase, vitamin C and diatomite, wherein, the vegetable oil that adds vehicle weight percentage 0.5~1% in diatomite, vegetable oil adopt a kind of in soya-bean oil, corn oil, peanut oil, cottonseed oil, the rapeseed oil; During preparation, in diatomite, add first vegetable oil, to be mixed evenly after, add successively Hypericum Perforatum P.E, vitamin C and phytase, after mixing, packing a kind of dilution type feed addictive of layer chicken.
The using method of dilution type feed addictive of layer chicken of the present invention is identical with embodiment 2.
Embodiment 4
A kind of feed addictive of layer chicken, the component of this additive comprises Hypericum Perforatum P.E, phytase, vitamin C and diatomite, and the weight proportion of the component of additive is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs, 400~600 parts of zeolite powders.
The preparation method of this feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, lucifuge is also cleaned, was soaked 2~4 hours with 35 ℃~55 ℃ temperature clear water after the harvesting, wherein, the hypericum perforatum plant is adopted the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding acetone, then extracted 20~40 minutes with ultrasonic wave under 20 ℃~40 ℃, dry filtered residue and the proportioning of acetone are: dry filtered residue 1kg, acetone 3000ml~8000ml; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase, vitamin C and zeolite powder, wherein, the vegetable oil that adds vehicle weight percentage 0.5~1% in zeolite powder, vegetable oil adopt a kind of in soya-bean oil, corn oil, peanut oil, cottonseed oil, the rapeseed oil; During preparation, in zeolite powder, add first vegetable oil, to be mixed evenly after, add successively Hypericum Perforatum P.E, vitamin C and phytase, after mixing, packing a kind of dilution type feed addictive of layer chicken.
The using method of dilution type feed addictive of layer chicken of the present invention is identical with embodiment 2.
Embodiment 5
A kind of feed addictive of layer chicken, the component of this additive comprises Hypericum Perforatum P.E, phytase, vitamin C and diatomite, and the weight proportion of the component of additive is: 100 parts of Hypericum Perforatum P.Es, 6~17 parts of phytases, 6~17 parts of vitamin Cs, 400~600 parts of zeolite powders.
The preparation method of this feed addictive of layer chicken is:
1, preparation Hypericum Perforatum P.E
The preparation process of Hypericum Perforatum P.E is:
(1) 15~25 centimetres of parts in hypericum perforatum plant top of gathering, lucifuge is also cleaned, was soaked 2~4 hours with 35 ℃~55 ℃ temperature clear water after the harvesting, wherein, the hypericum perforatum plant is adopted the hypericum perforatum that is planted in land for growing field crops, middle part, Zhejiang Province of introducing a fine variety from Part of The Qinling Mountains In Shaanxi Province north slope Wild plant;
(2) the hypericum perforatum plant after the immersion is removed stem and branch, gets leaf and flower section, and lucifuge is dried to water content and is lower than percentage by weight 0.05%;
(3) after drying hypericum perforatum leaf and flower section pulverizes with pulverizer, and hypericum perforatum leaf and flower section pulverize rear 60 mesh sieves;
(4) remove chlorophyll, above-mentioned hypericum perforatum powder put into can dissolve chlorophyllous solvent and mix, at 55 ℃~65 ℃ with ultrasonic grinder extraction 20~40 minutes, with the chlorophyll in the removal hypericum perforatum powder;
(5) filter extract, residue is put into baking oven under 55 ℃~65 ℃ temperature dry 2~4 hours, the NaOH that adds dried residue percentage by weight 0.1~0.3% in the dry residue, mix rear adding ethyl acetate, then extracted 20~40 minutes with ultrasonic wave under 20 ℃~40 ℃, dry filtered residue and the proportioning of ethyl acetate are: dry filtered residue 1kg, ethyl acetate 3000ml~8000ml; After the filtration, extract 55 ℃~65 ℃ ,-0.10~-0.06Mpa condition Vacuum Concentration, reclaim solvent, obtain the pitchy powder, cross 80 mesh sieves, be used for the Hypericum Perforatum P.E of feed addictive of layer chicken.This Hypericum Perforatum P.E is stored under lucifuge, 0~4 ℃ of condition.
Above all operations step all operates according under the intensity in lucifuge or than low light.
2, the preparation of feed addictive of layer chicken
Weight proportion by additive component takes by weighing Hypericum Perforatum P.E, phytase, vitamin C and zeolite powder, wherein, the vegetable oil that adds vehicle weight percentage 0.5~1% in zeolite powder, vegetable oil adopt a kind of in soya-bean oil, corn oil, peanut oil, cottonseed oil, the rapeseed oil; During preparation, in zeolite powder, add first vegetable oil, to be mixed evenly after, add successively Hypericum Perforatum P.E, vitamin C and phytase, after mixing, packing a kind of dilution type feed addictive of layer chicken.
The using method of dilution type feed addictive of layer chicken of the present invention is identical with embodiment 2.