CN101690454A - Russula vinosa Lindbl yield increasing fungi and using method thereof - Google Patents
Russula vinosa Lindbl yield increasing fungi and using method thereof Download PDFInfo
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Abstract
The invention provides russula vinosa Lindbl yield increasing fungi and a using method thereof, which belong to the technical field of russula vinosa Lindbl yield increase and solve the problems of low yield, high price, low quality and the like of wild russula vinosa Lindbl of the prior art. The russula vinosa Lindbl yield increasing fungi of the invention are a mixed flora of locally common ectomycorrhizal fungi and comprise Boletus brunneissimus Chiu, R. albida Peck, Boletus luridus and amanita yellow corner. Mycelia of various fungi of the russula vinosa Lindbl yield increasing fungi are cultured by a shaker culture method respectively; the mycelia are separate and used to prepare mycelium mixtures at a concentration of 50mg/L respectively; the mixtures are mixed uniformed in the same amount before inoculation to form the russula vinosa Lindbl yield increasing fungi; and on a woodland wherein russula vinosa Lindbl grows, the russula vinosa Lindbl yield increasing fungi are inoculated in dent pits by a root cutting and mycorrhization technique. In the method, ectomycorrhizal fungi flora is used to increase the biomass of thin roots of arbor, so the fruiting capacity, yield and quality of wild russula vinosa Lindbl are improved obviously and remarkable economic benefits are created. Thus, the method is suitable to be promoted and used in large areas.
Description
Technical field
The invention belongs to the Russula vinosa Lindbl yield-increasing technology, more specifically relate to Russula vinosa Lindbl yield increasing fungus and using method thereof.
Technical background
The symbiosis of ectotrophic mycorrhiza and trees is the important element of forest ecosystem foundation, evolution and stable development, especially in the blreal forest and the orohylile ecosystem, mycorrhizas homobium is indispensable component, as trees such as a genus of the Pinus of Pinaceae, Fagaceae, Fagus, Castaneas, all be must property ectotrophic mycorrhiza plant.Ectotrophic mycorrhiza infects the formed bacterium cover of short root of these trees and prolongs mycelia and mycorhiza network, is the real nutrient absorption organs of these trees.Under nature, there are not these trees of symbiosis of ectotrophic mycorrhiza almost can't survive.In forest ecosystem, the group of mycorrhizal fungi and the relation of plant community are so close, to such an extent as to any change of mycorrhizal fungi group all can cause the change of plant community ecology, and this change affects existence, competition and the diversity of mycorrhizal fungi conversely.
Russula vinosa Lindbl (Russula griseocarnosa X.H.Wang, Zhu L.Yang ﹠amp; Knudsen) being the ectotrophic mycorrhiza of a kind of symbiosis the Fagaceae trees, is a kind of delicious wild edible fungus (see figure 1) of Fujian Province simultaneously, is liked by the common people for a long time.Modern scientific research proof Russula vinosa Lindbl enriches blood really, anticancer, lipopenicillinase, protect health-care effects such as liver, Qiang Ti, is a kind of delicious food, healthy pollution-free food.Fujian Province is the suitable long district of China Fagaceae forest few in number, also is that Russula vinosa Lindbl is concentrated distribution ground.Do not feed owing to for a long time Russula vinosa Lindbl is only gathered; only eat and do not manage and protect; be embodied in the forest person of managing and protecting and the sylvan life Russula vinosa Lindbl picker two-areas of competence-separated; add that the picker is the mysteriousness that power is gathered in protection; it is very general to cause virgin mushroom collection and mushroom nest to destroy, and to the Russula vinosa Lindbl destruction Of resources and predatory the use, Fujian Province's Russula vinosa Lindbl resource has seriously been die-offed since the sixties in last century; not only Russula vinosa Lindbl the Fagaceae woods that gives birth to and is die-offed, and the density of Russula vinosa Lindbl also die-offs.The health care of Russula vinosa Lindbl constantly drapes over one's shoulders by scientific method and shows or confirm on the other hand, the consumer group is constantly enlarged, spurt in prices.
Russula vinosa Lindbl is not only the delicious food health care wild edible fungus of approving in a market; not only and the more important thing is the Russula vinosa Lindbl process of growth agricultural resources that accounts for out of question; there is not bacterium woods contradiction; and develop forestry; protection forestry; being and ecological protection, optimizing the high-quality industry that adapts with the human settlement, is the direction of mushroom industry development.
Main and the Fagaceae forest symbiosis of Russula vinosa Lindbl, but the tree radicula of living of its mycelia symbiosis diameter 0.05～0.8cm forms symbiont.Arid, acid rain, chemical fertilizer, agricultural chemicals, forest fire, harvest of forest trees etc. all can seriously reduce the fruiting amount of Russula vinosa Lindbl.
Summary of the invention
The purpose of this invention is to provide a kind of Russula vinosa Lindbl yield increasing fungus and using method thereof; Solve problems such as output is few in the wild Russula vinosa Lindbl collection in the prior art, price is high, quality is low, utilize the ectotrophic mycorrhiza flora to increase arbor radicula biomass, thereby reach the fruiting amount, output and the quality that significantly improve Russula vinosa Lindbl, have remarkable economic efficiency, be fit to apply in large area.
Russula vinosa Lindbl yield increasing fungus of the present invention is the mixed bacterial of Russula vinosa Lindbl ectotrophic mycorrhiza; Described mixed bacterial comprises brown bolete, white russule, Boletus luridus and yellow sharp squama Amanita fuliginea; Being prepared as of Russula vinosa Lindbl yield increasing fungus: adopt shaking table shaken cultivation method to cultivate the mycelium of four kinds of bacterium of Russula vinosa Lindbl yield increasing fungus respectively, attached to help thing to be mixed with concentration be 50mg.L with inoculation respectively
-1The mixed bacterial mixture of envelope-bulk to weight ratio (vermiculite, perlite, peat soil, sterile water were by 2: 1: 1: 0.5 mass ratio mixes and stirs), mixed in equal amounts becomes the yield increasing fungus flora before inoculation.
The using method of Russula vinosa Lindbl yield increasing fungus of the present invention is characterized in that: have on the forest land of Russula vinosa Lindbl growing, adopt the root Mycorrhizal technology of cutting, 4～6 meters one inoculation cave is carried out shallow cave and is inoculated described Russula vinosa Lindbl yield increasing fungus on the forest land.
Remarkable advantage of the present invention is:
(1) the present invention makes full use of rational ectotrophic mycorrhiza flora increases arbor radicula biomass; use this bacterium on Russula vinosa Lindbl protection ground, can increase substantially the radicula biomass of Fagaceae woods, increase substantially the amount of gathering of Russula vinosa Lindbl then; and can promote forest development, can be applicable to agriculture and forestry.
(2) the present invention is according to ecological mycorhiza diversity principle; increase considerably the arbor radicula biomass on Russula vinosa Lindbl protection ground by Russula vinosa Lindbl yield increasing fungus and secretion thereof, and then increase substantially the output of the ectotrophic mycorrhiza edible mushroom Zhao Russula vinosa Lindbl of symbiosis on the arbor radicula.Present technique can increase substantially healthy food Russula vinosa Lindbl output, and can promote forest development.Can be applicable to agriculture and forestry
(3) the present invention also has following four big characteristics, and the one, biological, the various bacterium in the Russula vinosa Lindbl yield increasing fungus are common at natural world, use back long-term surviving and amplification in woods, can promote the radicula amount of growth in forest zone to increase and the raising of mycorhiza symbiosis rate, and then improve Russula vinosa Lindbl output, pure green technology; The 2nd, long-lasting, volume increase all the life behind the applied once Russula vinosa Lindbl yield increasing fungus, inject forest zone ecology the Russula vinosa Lindbl yield increasing fungus multiply on the spot, constantly promote the growth of forest in the ecosystem and the volume increase of Russula vinosa Lindbl; The 3rd, high efficiency, technology are implemented the density of Russula vinosa Lindbl, output are all increased substantially, and amount of increase in production can be up to 200%, and increasing both production and income increases substantially farmers' income; The 4th, low-cost, technology is easy to learn, implements the stepmother and needs additional management, technical costs and labour costs are all very cheap, thereby reach the quality of the fruiting amount, output and the Russula vinosa Lindbl that significantly improve Russula vinosa Lindbl, have remarkable economic efficiency, be fit to apply in large area.
(4) up to the present, Russula vinosa Lindbl can't artificial cultivation, and method of the present invention is that Russula vinosa Lindbl is increased production effective method.
Description of drawings
Fig. 1 is the electron lens photo of Russula vinosa Lindbl fruit body and spore thereof.
Fig. 2 is brown bolete fruit body photo.
Fig. 3 is white russule fruit body photo.
Fig. 4 is a Boletus luridus fruit body photo.
Fig. 5 is yellow sharp squama Amanita fuliginea fruit body photo.
Fig. 6 is that the Russula vinosa Lindbl scene photograph is cultivated in forestry, place etc. on the spot.
Fig. 7 HPLC associating biology determination and analysis goes out with radicula quantity to increase closely-related 2 peak values.
Fig. 8 filters out corresponding peaks HPLC figure high and other 3 bacterium of promotion radicula biomass obvious results in mensuration.
Embodiment
The Russula vinosa Lindbl yield increasing fungus is the mixed bacterial of Russula vinosa Lindbl ectotrophic mycorrhiza; Described mixed bacterial comprises brown bolete, white russule, Boletus luridus and yellow sharp squama Amanita fuliginea.
Brown bolete Boletus aereus Fr. (Chinese fungi will the 22 volume Boletaceae (I)), the cap hemispherical is flat hemispherical extremely, diameter 7-15cm, the steel helmet shape, gradually open and flat after the maturation, roll up slightly on the limit.Brown is covered microvilli to taupe.The bacterial context near-white, thick, heavily fortified point when tender, soft slightly after ripe, hinder the yellowish-brown that shoals; Tube white is to lightpink, and is near straight living extremely closely from life, nozzle diameter 0.5-1mm, canescence, single hole, circle, long 4-8mm.Stem is cylindrical, and general top is thinner, sometimes in or the bottom expand, or go up inferior thick, long 4-9cm, thick 1.5-5cm, approximate cap look or top look shallow, there is the coarse reticulate pattern of dark brown on the surface, inside is solid.Spore is smooth, the nearly fusiformis of oblong, 9-12 μ m.The nearly spindle of pipe side utricule.Summer and autumn and be born in the oakery face of land.
Bai Honggu Russula albida Peck (Chinese macro fungi primary colors collection of illustrative plates, p222.) single living or scattered, bacteria cover diameter 2.5-6cm, white, flat, central authorities are recessed slightly, and epidermis is sticking, easily openable, bacterial context white, crisp, the recessed life of lamella or straight living, white, close or close slightly, the length unanimity has transverse vein between pleat, handle 2.2-5.5cm*3-8*0.8-2cm, white, smooth Gun Feng shape, inner soft, back hollow.The spore print milk yellow, spore is colourless, and subsphaeroidal or near oval has little wart, wart Jian Xiang Even sometimes, 6.4-8.7*5.5-7.3 μ m, cystidium prismatic.
Boletus luridus Boletus luridus Fr. (Chinese macro fungi primary colors collection of illustrative plates) bacteria cover diameter 3-12cm, semicircle to open and flat steel helmet shape, dry tack free, tool fine hair or very thin scale, yellow or brick yellow, the lid edge is orange-yellow; Meat is light yellow to little red, hinders to become blue, and tube is elongated, yellow, and the hole is little and justify, and light red is shown in the hole.Handle 5-13cm, bar-shaped, wait slightly, red or yellowish pink, visible sometimes reticulate pattern.Basidiospore 12-17 μ m*5.5-7 μ m, narrow avette, asymmetric.
Yellow sharp squama Amanita fuliginea Amaita xanthogola Bas (Chinese macro fungi primary colors collection of illustrative plates) bacteria cover diameter 6-13cm, the flat hemispherical of hemispherical is to open and flat, fawn or shallow isabelline, tool angle vertebra shape scale and cotton-shaped scale, edge-smoothing or rill is arranged and the normal cotton-shaped adjunct of tool.Bacterial context white, the yellow or pale yellow buff that arrives in lamella hole, from life, line is wide, and is not isometric, and the long 12-16cm of stem, thick 1.5-2.6cm, base portion expand and locate to reach 3cm, and extend into the root shape, with lid look or shallow slightly, solid.Spore is colourless, ellipse, load 4 stigmas.The food poison is not clear.
Below prove absolutely the present invention according to a particular embodiment of the invention, but the present invention is not limited only to this.
Specific embodiment
Wherein brown bolete fruit body is from Yunnan Province's Lufeng County, and white russule, Boletus luridus and yellow sharp squama Amanita fuliginea fruit body are all from the new counties and townships of Hanjiang District rice paper mulberry woods.
Adopt the mycelium of the described various bacterium of shaking table shaken cultivation:
The mycelium isolation medium: potato 20 grams, glucose 20 grams, squama acid dihydride potassium 3 grams, magnesium sulfate 0.3 gram, agar 18 grams add water to 1 liter;
Separating method: gather new fresh sporophore, under gnotobasis, gather the pure bacterial context of stem or lid, move to above-mentioned isolation medium, be cultured under 26-28 ℃ grow mycelia after, move the pipe purifying and cultivate, observe record and cultivate proterties; Be seeded in vitro the aseptic Fagaceae shoot root portion of cultivating, 2 week the back observe mycorhiza, move≤mycorhiza of 1mm size observes and relatively cultivate proterties to above-mentioned isolation medium; The two cultivates the proterties unanimity, then carries out mycelia and segregant entity dna sequence analysis to determine autoploidy.
The mycelium liquid medium: potato 20 grams, glucose 20 grams, squama acid dihydride potassium 3 grams, magnesium sulfate 0.3 gram adds water to 1 liter
The mycelium liquid cultural characteristic of brown bolete: pH scope 5.5-6.0, temperature 26-29 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 7 days;
The mycelium of Bai Honggu is cultivated: pH scope 5.5-6, and temperature range 26-29 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 7 days;
The mycelium of Boletus luridus is cultivated: pH scope .6-6.5, and temperature range 26-28 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 6 days;
The mycelium of yellow sharp squama Amanita fuliginea is cultivated: pH scope 5-6, and temperature range 26-28 ℃, rotating speed 120-150, the shaking table shaken cultivation was cultivated 14-16 days.
The potassium of normal concentration, magnesium, calcium, iron, copper, manganese, zinc various element all do not influence mycelial growth.
Adopt shaking table shaken cultivation method to cultivate the mycelium of the various bacterium of Russula vinosa Lindbl yield increasing fungus respectively, disperse the back to add the attached thing that helps of inoculation respectively, being mixed with concentration is 50mg.L
-1The mycelium inoculation thing of (concentration is according to various bright mycelia weight/volumes), described inoculation is attached help thing be vermiculite, perlite, peat soil, sterile water by 2: 1: 1: 0.5 mass ratio mixes also and stirs, and the mycelium inoculation thing mixed in equal amounts of various bacterium becomes the yield increasing fungus flora before inoculation.(Luan Qingshu etc., the research of efficient ectotrophic mycorrhiza bacteria preparation, Liaoning forestry science and technology, 2003 (6))
The using method of Russula vinosa Lindbl yield increasing fungus: have on the forest land of Russula vinosa Lindbl growing, root Mycorrhizal technology is cut in employing, at a distance of 4～6 meters one inoculation cave, with apart 1.5～2.0 meters of inoculation elite stands, gently cut out top layer surface dust and leaf to the radicula of living and palm size of area occurs simultaneously, the degree of depth is in 10cm, if do not dig radicula, then change a position and dig, on the forest land, carry out shallow cave and inoculate described Russula vinosa Lindbl yield increasing fungus.
The consumption of Russula vinosa Lindbl yield increasing fungus is: the described mixed bacterial mycelia mixture of the yield increasing fungus of 10-15ml volume is poured in each inoculation cave into; The degree of depth 2-10cm in described inoculation cave; Diameter 15-12cm, and have elite stand to live radicula in the inoculation cave, mixed bacterial mycelia mixture accurately surrounds and is positioned over the radicula periphery.Fill and lead up the inoculation cave after the inoculation in case the diffusing inoculum of wind.
Observe record: connect the annual variation of observing the radicula biomass behind the bacterium.And weigh by whole Russula vinosa Lindbl oven dry that test region and check plot are collected in mushroom farming, dry to constant weight post analysis balance with random sampling and weigh tender positive Russula vinosa Lindbl fruit body collection capacity.
Example is cultivated in forestry, place etc. on the spot
The technology implementation specification: one encloses, and finishes the bamboo fence enclosure wall of russule woods in the past in April; Two see, promptly see soil temperature and see soil moisture, and the soil temperature when guaranteeing to inoculate is in (generally before and after the Dragon Boat Festival) more than 26 ℃, and humidity is more than 90%, or have a saturating rain after the inoculation, make bacterium can successfully enter host's radicula; Three technology, employing cuts a root Mycorrhizal technology during inoculation, and 5 meters optimum density technology and composite flora inoculation technique are deposited in this bacterium around the radicula of soil table 5-10cm layer at interval, guarantee success rate of inoculating and effective promotion that radicula is grown; Four checks are looked into the mycorhiza rate, look into the radicula biomass, look into Russula vinosa Lindbl density and are looked into Russula vinosa Lindbl output after the inoculation, guarantee success ratio of inoculation.The auspicious concrete case study on implementation of seeing is operated in inoculation.
The russule mountain that case one, Hanjiang District ocean township Lian Feng village area are 12 mu, surrounding 2 sections of 6 mu with the condition homalographic with the bamboo hedge tests and contrasts, two districts all are divided into each 3 sub-district of 2 mu of area successively along the slope direction, microbial inoculum adopts the mycelium of the Russula vinosa Lindbl yield increasing fungus of shaking table shaken cultivation, breaks up that back and inoculation are attached to be helped thing to be mixed with concentration to be about 50mgL
-1The mycelia mixture, and adopt the flower root Mycorrhizal technology of cutting that dawn, plum was introduced, the inoculation of shallow cave is carried out in an inoculation of 4-6 rice cave on the forest land.Mixing the attached thing that helps of inoculation with sterile water is contrast.Connect the annual variation of observing the radicula biomass behind the bacterium.And weigh by whole Russula vinosa Lindbl oven dry that test region and check plot are collected in mushroom farming, dry to constant weight post analysis balance with random sampling and weigh tender positive Russula vinosa Lindbl fruit body collection capacity.
1. 0-10cm soil layer arbor radicula quantity sees Table 1 before and after the inoculation of Russula vinosa Lindbl yield increasing fungus, and the quantity increase is very remarkable.The radicula biomass data in totally 6 years in test processing front and back see Table 2, the radicula biomass reached the highest on the 3rd year in inoculation as can be seen, substantially maintain this radicula biomass level,, slightly descend so compare because concurrent control district radicula biomass still is and increases trend year by year thereafter.
Table 1 is distinguished 0-10cm soil layer arbor radicula quantity (unit: prop up/0.385cm for examination before and after connecing the bacterium processing
2)
Table 2 connects bacterium and handles the variation of front and back for the average radicula biomass in examination district
2.2005 the Russula vinosa Lindbl fruit body density that year onsite acceptance is measured sees Table 3,5 the average fruit body numbers in test region are 12/0.785m
2, 5 the average fruit body numbers in check plot are 6/0.785m
2, the density ratio is 2: 1.
The onsite acceptance of table 3 russule fruit body density (piece/0.785m
2)
The Russula vinosa Lindbl output of 3 test regions and check plot is compared to table 4, and to inoculating back the 4th year, the highest can the reaching of Russula vinosa Lindbl collection capacity doubled.Comparative observation afterwards shows that russule output is stabilized in this yield level basically and does not descend that the increase that also shows russule output is to be associated with the increase of radicula biomass.
Supply two russule woodss year output contrasts (kilogram/hectare) of examination before and after table 4 test
4. the correlation analysis of radicula biomass and Russula vinosa Lindbl output
The correlation analysis (table 5) of radicula biomass and Russula vinosa Lindbl output shows that the radicula biomass and the Russula vinosa Lindbl output of 0-100cm soil layer are proportionate, and correlation coefficient reaches 0.9627.
The correlation analysis of table 5 radicula biomass and Russula vinosa Lindbl output
Effect principle laboratory test to Russula vinosa Lindbl yield increasing fungus of the present invention:
The Russula vinosa Lindbl yield increasing fungus promotes the material (metabolic characteristics) of radicula growth:
The operation principle of Russula vinosa Lindbl yield increasing fungus is: forest zone inoculation volume increase bacterial classification Zhao enters the radicula system, and promoting radicula quantity to increase (unit are Russula vinosa Lindbl mycelia amount increases) Zhao Russula vinosa Lindbl density significantly at forest zone breeding Zhao increases, increase substantially the Russula vinosa Lindbl amount of gathering.
Use the HPLC technology and supply the examination bacterium in conjunction with the biology determination and analysis, find with radicula quantity and increase closely-related 2 peak value (see figure 7)s, and filter out according to this radicula biomass facilitation effect remarkable (Fig. 8), promote the very significant one group of bacterium of the Russula vinosa Lindbl amount of gathering increase in practice.
The Russula vinosa Lindbl yield increasing fungus reaches the antagonistic effect with Russula vinosa Lindbl between any two, and the result shows, the Russula vinosa Lindbl yield increasing fungus reaches between they and the Russula vinosa Lindbl does not between any two all have the reaction of the antagonism of appearance.Guarantee postvaccinal compatibility and the object bacteria Russula vinosa Lindbl is had no adverse effect extremely.
Claims (6)
1. Russula vinosa Lindbl yield increasing fungus, it is characterized in that: described Russula vinosa Lindbl yield increasing fungus is the mixed bacterial of the common ectotrophic mycorrhiza in forest zone; Described mixed bacterial comprises brown bolete, white russule, Boletus luridus and yellow sharp squama Amanita fuliginea.
2. Russula vinosa Lindbl yield increasing fungus according to claim 1, it is characterized in that: being prepared as of described Russula vinosa Lindbl yield increasing fungus: adopt the method for shaking table shaken cultivation to cultivate the mycelium of the various bacterium of Russula vinosa Lindbl yield increasing fungus respectively, help thing to be mixed mutually to make the mycelia mixture that concentration is 50mg/L with inoculation is attached respectively after breaing up, concentration is according to various bright mycelia weight/volume of mixture, and mixed in equal amounts becomes the yield increasing fungus flora before inoculation.
3. Russula vinosa Lindbl yield increasing fungus according to claim 2, it is characterized in that: add the attached thing that helps of inoculation after the mycelia of described four bacterium is broken up respectively in proportion, described inoculation is attached help thing be vermiculite, perlite, peat soil, sterile water by 2: 1: 1: 0.5 mass ratio mixes also and stirs, and mixed in equal amounts becomes the yield increasing fungus flora before inoculation.
4. Russula vinosa Lindbl yield increasing fungus according to claim 2 is characterized in that: the mycelium process of the various bacterium of cultivating the Russula vinosa Lindbl yield increasing fungus respectively of described employing shaking table shaken cultivation method is:
The mycelium isolation medium: potato 20 grams, glucose 20 grams, squama acid dihydride potassium 3 grams, magnesium sulfate 0.3 gram, agar 18 grams add water to 1 liter;
The separating method of various bacterium: gather new fresh sporophore, under gnotobasis, gather the pure bacterial context of stem or cap, move to above-mentioned isolation medium, be cultured under 26-28 ℃ grow mycelia after, single mycelia moves the pipe purifying and cultivates, and observes record and cultivates proterties.With the mycelium inoculation after the separation and purification to the aseptic Fagaceae shoot root portion of in vitro cultivating, 2 weeks back observation mycorhiza, the mycorhiza of intercepting≤1mm size is to isolation medium, observe and relatively cultivate proterties, the two cultivates the proterties unanimity, carry out mycelia and segregant entity dna sequence analysis again, further to determine autoploidy;
The mycelium liquid medium: potato 20 grams, glucose 20 grams, squama acid dihydride potassium 3 grams, magnesium sulfate 0.3 gram adds water to 1 liter;
The mycelium liquid cultural characteristic of brown bolete: pH scope 5.5-6.0, temperature 26-29 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 7 days;
The mycelium of Bai Honggu is cultivated: pH scope 5.5-6, and temperature range 26-29 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 7 days;
The mycelium of Boletus luridus is cultivated: pH scope .6-6.5, and temperature range 26-28 ℃, rotating speed 120-150rpm, the shaking table shaken cultivation was cultivated 6 days;
The mycelium of yellow sharp squama Amanita fuliginea is cultivated: pH scope 5-6, and temperature range 26-28 ℃, rotating speed 120-150, the shaking table shaken cultivation was cultivated 14-16 days.
5. the using method of a claim 1,2,3,4 or 5 described Russula vinosa Lindbl yield increasing funguses, it is characterized in that: have on the forest land of Russula vinosa Lindbl growing, root Mycorrhizal technology is cut in employing, at a distance of 4～6 meters one inoculation cave, simultaneously with apart 1.5～2.0 meters of inoculation elite stands, gently cutting out top layer surface dust and leaf to the radicula of living occurs, palm size of area, the degree of depth are in 10cm, when not digging radicula, change a position and dig, on the forest land, carry out shallow cave and inoculate described Russula vinosa Lindbl yield increasing fungus.
6. the using method of Russula vinosa Lindbl yield increasing fungus according to claim 9 is characterized in that: the consumption of described Russula vinosa Lindbl yield increasing fungus is that the described mixed bacterial mycelia mixture of the yield increasing fungus of 10～15ml volume is poured in each inoculation cave into; The degree of depth 2～the 10cm in described inoculation cave, diameter 15～12cm, and have elite stand to live radicula in the inoculation cave; Yield increasing fungus accurately is positioned over radicula periphery alive, fills and leads up the inoculation cave after the inoculation, prevents that bacterial classification is blown to four winds.
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| CN101953273A (en) * | 2010-11-01 | 2011-01-26 | 湖南省林业科学院 | Production increasing method for bolete in forests |
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| CN103609338A (en) * | 2013-12-19 | 2014-03-05 | 云南大学 | Brick-red xerocomus silwoodensis test tube sporocarp primordium inducing method |
| CN103609338B (en) * | 2013-12-19 | 2015-09-30 | 云南大学 | The abductive approach of brick red suede lid bolete test tube fruit body primordium |
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| CN103980060A (en) * | 2014-06-05 | 2014-08-13 | 尤溪县林业科技推广中心 | Chinese amanita fuliginea culture medium as well as preparation method and application thereof |
| CN104106376A (en) * | 2014-07-16 | 2014-10-22 | 福建省农业科学院食用菌研究所 | Russula griseocarnosa yield increase method by adjusting mycorrhiza and fungus community structures |
| CN105103947A (en) * | 2015-08-20 | 2015-12-02 | 陈宇航 | Using method of special bacterium source formatted by ectotrophic mycorrhiza under natural forest |
| WO2017028767A1 (en) * | 2015-08-20 | 2017-02-23 | 陈宇航 | Method of using specialized fungus source formed from ectomycorrhiza under natural forest |
| CN106212042A (en) * | 2016-07-04 | 2016-12-14 | 李业武 | A kind of pseudo-wild cultivating method of Tricholoma matsutake (lto et lmai) Singer |
| CN107318467A (en) * | 2017-08-23 | 2017-11-07 | 佛山推启农业研究院（普通合伙） | A kind of method of the blood red bolete of artificial cultivation |
| CN110024615A (en) * | 2019-03-25 | 2019-07-19 | 广西壮族自治区林业科学研究院 | The method that field forest land wheel cuts down strengthening root and controls earth's surface temperature and humidity production Russula vinosa |
| CN110972811A (en) * | 2019-12-23 | 2020-04-10 | 云南农业大学 | Method for establishing DSE (DSE-activated carbon), AMF (Amf) and plant symbiotic system and application of DSE-AMF and plant symbiotic system |
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