CN101684500A - 测定结肠癌 - Google Patents
测定结肠癌 Download PDFInfo
- Publication number
- CN101684500A CN101684500A CN200910175739A CN200910175739A CN101684500A CN 101684500 A CN101684500 A CN 101684500A CN 200910175739 A CN200910175739 A CN 200910175739A CN 200910175739 A CN200910175739 A CN 200910175739A CN 101684500 A CN101684500 A CN 101684500A
- Authority
- CN
- China
- Prior art keywords
- seq
- gene
- people
- expression
- molectron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010009944 Colon cancer Diseases 0.000 title claims abstract description 30
- 208000001333 Colorectal Neoplasms Diseases 0.000 title claims abstract description 29
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 133
- 230000014509 gene expression Effects 0.000 claims abstract description 73
- 238000002493 microarray Methods 0.000 claims abstract description 24
- 206010028980 Neoplasm Diseases 0.000 claims description 27
- 201000010989 colorectal carcinoma Diseases 0.000 claims description 26
- 239000002299 complementary DNA Substances 0.000 claims description 24
- 238000012360 testing method Methods 0.000 claims description 17
- 238000003745 diagnosis Methods 0.000 claims description 16
- 238000011282 treatment Methods 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 201000011510 cancer Diseases 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 8
- 210000001072 colon Anatomy 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 7
- 150000007523 nucleic acids Chemical group 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 208000029742 colonic neoplasm Diseases 0.000 claims description 3
- 102000000820 Enterotoxin Receptors Human genes 0.000 claims description 2
- 108010001687 Enterotoxin Receptors Proteins 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 238000010208 microarray analysis Methods 0.000 claims description 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 38
- 239000000523 sample Substances 0.000 description 53
- 108020004414 DNA Proteins 0.000 description 50
- 230000002068 genetic effect Effects 0.000 description 30
- 108020004635 Complementary DNA Proteins 0.000 description 23
- 238000010804 cDNA synthesis Methods 0.000 description 23
- 210000004027 cell Anatomy 0.000 description 23
- 230000008859 change Effects 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 13
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000000370 laser capture micro-dissection Methods 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000004393 prognosis Methods 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 210000005259 peripheral blood Anatomy 0.000 description 5
- 239000011886 peripheral blood Substances 0.000 description 5
- 210000002826 placenta Anatomy 0.000 description 5
- 239000002243 precursor Substances 0.000 description 5
- 206010006187 Breast cancer Diseases 0.000 description 4
- 208000026310 Breast neoplasm Diseases 0.000 description 4
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 4
- 108700005075 Regulator Genes Proteins 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000005357 flat glass Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000012340 reverse transcriptase PCR Methods 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 210000004907 gland Anatomy 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Substances N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 3
- 210000004400 mucous membrane Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 102100037965 60S ribosomal protein L21 Human genes 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108020004394 Complementary RNA Proteins 0.000 description 2
- 102000029792 Desmoplakin Human genes 0.000 description 2
- 108091000074 Desmoplakin Proteins 0.000 description 2
- 102100032822 Homeodomain-interacting protein kinase 1 Human genes 0.000 description 2
- 101001066404 Homo sapiens Homeodomain-interacting protein kinase 1 Proteins 0.000 description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000030279 gene silencing Effects 0.000 description 2
- 238000012226 gene silencing method Methods 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 239000006101 laboratory sample Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000012775 microarray technology Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 239000013610 patient sample Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 108090000327 ribosomal protein L21 Proteins 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 101150039504 6 gene Proteins 0.000 description 1
- 102100033936 AP-3 complex subunit beta-1 Human genes 0.000 description 1
- 101710165439 AP-3 complex subunit beta-1 Proteins 0.000 description 1
- 206010001167 Adenocarcinoma of colon Diseases 0.000 description 1
- 108060000903 Beta-catenin Proteins 0.000 description 1
- 102000015735 Beta-catenin Human genes 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000252983 Caecum Species 0.000 description 1
- 101100422242 Caenorhabditis elegans sqv-7 gene Proteins 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
- 206010007275 Carcinoid tumour Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102100021934 Cyclin-D1-binding protein 1 Human genes 0.000 description 1
- 102100028843 DNA mismatch repair protein Mlh1 Human genes 0.000 description 1
- 102100034157 DNA mismatch repair protein Msh2 Human genes 0.000 description 1
- 102100021147 DNA mismatch repair protein Msh6 Human genes 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102100027297 Fatty acid 2-hydroxylase Human genes 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 1
- 101000897488 Homo sapiens Cyclin-D1-binding protein 1 Proteins 0.000 description 1
- 101001134036 Homo sapiens DNA mismatch repair protein Msh2 Proteins 0.000 description 1
- 101000968658 Homo sapiens DNA mismatch repair protein Msh6 Proteins 0.000 description 1
- 101000937693 Homo sapiens Fatty acid 2-hydroxylase Proteins 0.000 description 1
- 101000738901 Homo sapiens PMS1 protein homolog 1 Proteins 0.000 description 1
- 101000685275 Homo sapiens Protein sel-1 homolog 1 Proteins 0.000 description 1
- 101000825182 Homo sapiens Transcription factor Spi-B Proteins 0.000 description 1
- 101100483382 Homo sapiens UBE2J1 gene Proteins 0.000 description 1
- 101150062179 II gene Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 1
- 229910015837 MSH2 Inorganic materials 0.000 description 1
- 208000032818 Microsatellite Instability Diseases 0.000 description 1
- 108010074346 Mismatch Repair Endonuclease PMS2 Proteins 0.000 description 1
- 102100037480 Mismatch repair endonuclease PMS2 Human genes 0.000 description 1
- 108010026664 MutL Protein Homolog 1 Proteins 0.000 description 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 101710157860 Oxydoreductase Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 102100037482 PMS1 protein homolog 1 Human genes 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 102100023159 Protein sel-1 homolog 1 Human genes 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 102100022281 Transcription factor Spi-B Human genes 0.000 description 1
- 102100024860 Ubiquitin-conjugating enzyme E2 J1 Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 108010061314 alpha-L-Fucosidase Proteins 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- 101150036080 at gene Proteins 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 208000002458 carcinoid tumor Diseases 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 208000029664 classic familial adenomatous polyposis Diseases 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 201000010897 colon adenocarcinoma Diseases 0.000 description 1
- 230000000112 colonic effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 238000003167 genetic complementation Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000002500 microbody Anatomy 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003499 nucleic acid array Methods 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000011524 similarity measure Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36868702P | 2002-03-29 | 2002-03-29 | |
| US60/368687 | 2002-03-29 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031378714A Division CN1502989A (zh) | 2002-03-29 | 2003-03-29 | 测定结肠癌 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101684500A true CN101684500A (zh) | 2010-03-31 |
Family
ID=28675528
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910175739A Pending CN101684500A (zh) | 2002-03-29 | 2003-03-29 | 测定结肠癌 |
| CNA031378714A Pending CN1502989A (zh) | 2002-03-29 | 2003-03-29 | 测定结肠癌 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031378714A Pending CN1502989A (zh) | 2002-03-29 | 2003-03-29 | 测定结肠癌 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20030186303A1 (enExample) |
| EP (1) | EP1355151A3 (enExample) |
| JP (2) | JP4354725B2 (enExample) |
| KR (1) | KR100984996B1 (enExample) |
| CN (2) | CN101684500A (enExample) |
| AR (1) | AR039211A1 (enExample) |
| AU (1) | AU2003203561A1 (enExample) |
| BR (1) | BR0303012A (enExample) |
| CA (1) | CA2422305C (enExample) |
| MX (1) | MXPA03002863A (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586423A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测结直肠癌易感基因的方法及试剂盒 |
| CN102586420A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测乳腺癌易感基因的方法及试剂盒 |
| CN115678994A (zh) * | 2022-01-27 | 2023-02-03 | 上海爱谱蒂康生物科技有限公司 | 一种生物标志物组合、含其的试剂及其应用 |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2475769C (en) * | 2003-08-28 | 2018-12-11 | Veridex, Llc | Colorectal cancer prognostics |
| US20050153352A1 (en) * | 2004-01-09 | 2005-07-14 | Stanley Chang | Cancer specific gene MG20 |
| CA2610709A1 (en) * | 2005-06-06 | 2006-12-14 | Genentech, Inc. | Transgenic models for different genes and their use for gene characterization |
| TW200730825A (en) * | 2005-10-21 | 2007-08-16 | Genenews Inc | Method and apparatus for correlating levels of biomarker products with disease |
| CN101437962A (zh) * | 2006-03-03 | 2009-05-20 | 维里德克斯有限责任公司 | 预测杜克氏b结肠癌复发的分子检测 |
| CN102099485A (zh) * | 2007-10-23 | 2011-06-15 | 临床基因组学有限公司 | 诊断新生物的方法-ⅱ |
| EP2169078A1 (en) | 2008-09-26 | 2010-03-31 | Fundacion Gaiker | Methods and kits for the diagnosis and the staging of colorectal cancer |
| WO2013092960A1 (en) | 2011-12-22 | 2013-06-27 | Fundacion Gaiker | Methods and kits for the diagnosis of colorectal cancer |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US635152A (en) * | 1899-06-05 | 1899-10-17 | Melancthon S Shotwell | Car-body bolster. |
| US6275814B1 (en) * | 1996-11-27 | 2001-08-14 | Investment Strategies Network | Investment portfolio selection system and method |
| US6003018A (en) * | 1998-03-27 | 1999-12-14 | Michaud Partners Llp | Portfolio optimization by means of resampled efficient frontiers |
| US6350578B1 (en) * | 1999-06-25 | 2002-02-26 | The Regents Of The University Of California | Method of quantitating dsDNA |
| US6175824B1 (en) * | 1999-07-14 | 2001-01-16 | Chi Research, Inc. | Method and apparatus for choosing a stock portfolio, based on patent indicators |
-
2003
- 2003-03-21 US US10/394,382 patent/US20030186303A1/en not_active Abandoned
- 2003-03-28 BR BR0303012-1A patent/BR0303012A/pt not_active IP Right Cessation
- 2003-03-28 CA CA2422305A patent/CA2422305C/en not_active Expired - Fee Related
- 2003-03-28 AU AU2003203561A patent/AU2003203561A1/en not_active Abandoned
- 2003-03-29 KR KR1020030019815A patent/KR100984996B1/ko not_active Expired - Fee Related
- 2003-03-29 CN CN200910175739A patent/CN101684500A/zh active Pending
- 2003-03-29 CN CNA031378714A patent/CN1502989A/zh active Pending
- 2003-03-31 JP JP2003094744A patent/JP4354725B2/ja not_active Expired - Fee Related
- 2003-03-31 EP EP03252024A patent/EP1355151A3/en not_active Withdrawn
- 2003-03-31 MX MXPA03002863A patent/MXPA03002863A/es active IP Right Grant
- 2003-04-01 AR ARP030101132A patent/AR039211A1/es not_active Application Discontinuation
-
2009
- 2009-03-31 JP JP2009086981A patent/JP2009153521A/ja active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586423A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测结直肠癌易感基因的方法及试剂盒 |
| CN102586420A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测乳腺癌易感基因的方法及试剂盒 |
| CN115678994A (zh) * | 2022-01-27 | 2023-02-03 | 上海爱谱蒂康生物科技有限公司 | 一种生物标志物组合、含其的试剂及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003203561A1 (en) | 2003-10-23 |
| CA2422305A1 (en) | 2003-09-29 |
| AR039211A1 (es) | 2005-02-09 |
| JP2003325191A (ja) | 2003-11-18 |
| BR0303012A (pt) | 2004-09-08 |
| KR20030078801A (ko) | 2003-10-08 |
| CN1502989A (zh) | 2004-06-09 |
| KR100984996B1 (ko) | 2010-10-04 |
| CA2422305C (en) | 2013-07-30 |
| EP1355151A3 (en) | 2004-08-25 |
| EP1355151A2 (en) | 2003-10-22 |
| JP4354725B2 (ja) | 2009-10-28 |
| JP2009153521A (ja) | 2009-07-16 |
| US20030186303A1 (en) | 2003-10-02 |
| MXPA03002863A (es) | 2004-08-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10889865B2 (en) | Thyroid tumors identified | |
| CA2442820A1 (en) | Microarray gene expression profiling in clear cell renal cell carcinoma: prognosis and drug target identification | |
| US20040241725A1 (en) | Lung cancer detection | |
| KR100984996B1 (ko) | 결장직장암의 검사방법 | |
| CN101603092B (zh) | 评估结肠直肠癌 | |
| AU2016377391B2 (en) | Triage biomarkers and uses therefor | |
| CA3067730A1 (en) | Methods for detection of plasma cell dyscrasia | |
| WO2007135174A1 (de) | Praediktives genexpressionsmuster fuer kolorektale karzinome | |
| US20070298419A1 (en) | K-Ras Oligonucleotide Microarray and Method for Detecting K-Ras Mutations Employing the Same | |
| KR20210122017A (ko) | 색소침착 피부 타입 판단용 유전자 다형성 마커 및 이의 용도 | |
| CN1856573A (zh) | 用于神经母细胞瘤预后诊断的微阵列和神经母细胞瘤预后诊断方法 | |
| KR101774275B1 (ko) | 고추의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도 | |
| KR20220141658A (ko) | 피부색 판단용 유전자 다형성 마커 및 이의 용도 | |
| CN1985004A (zh) | 监测丝状真菌细胞中基因表达的方法 | |
| JP2008263971A (ja) | ラクトバチラス属乳酸菌の検出用プライマーおよびプローブ | |
| ten Asbroek et al. | Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome | |
| JP2002112799A (ja) | 癌疾患の予後判定法 | |
| KR20220137382A (ko) | 주름 피부 타입 판단용 유전자 다형성 마커 및 이의 용도 | |
| KR101139360B1 (ko) | Ｐｒｋｃｉ, ｍａｐｋ10, ｓｐｐ1, ｉｑｇａｐ2, ｆｇｆｒ4, ｎｏｔｃｈ4, ｈｌａ-ｄｒａ, ｈｌａ-ｄｏａ, ｔｈｂｓ2, ｄｆｎａ5, ｔｂｘａｓ1, ｔｎｋｓ, ｃｄｈ17, ｕｂｒ5, ｋｉａａ0196, 및 ｎｓｍｃｅ2 유전자로부터 유래된 단일염기다형을 포함하는 폴리뉴클레오티드, 이를 포함하는 마이크로어레이 및 진단키트, 및 이를 이용한 분석방법 | |
| US20070059714A1 (en) | Detection of presence and antibiotic susceptibility of enterococci | |
| KR20220141659A (ko) | 피부색 판단용 유전자 다형성 마커 및 이의 용도 | |
| KR20110043100A (ko) | Ａｎｋｒｄ15, ｈｐｄ, ｐｓｍｄ9, ｗｄｒ66, ｇｐｃ6, ｐａｘ9, ｌｒｒｃ28, ｔｎｓ4, ａｘｌ, 및 ｈｎｒｐｕｌ1 유전자로부터 유래된 단일염기다형을 포함하는 폴리뉴클레오티드, 이를 포함하는 마이크로어레이 및 진단키트, 및 이를 이용한 분석방법 | |
| KR20230036927A (ko) | 아토피 피부염의 발병 위험도 예측 또는 진단을 위한 정보제공 방법 | |
| KR20130100640A (ko) | 복부비만 예측용 ｓｎｐ 마커 및 이의 용도 | |
| Class et al. | Patent application title: METHODS FOR THE DETECTION OF SEQUENCE AMPLIFICATION IN THE BRCA1 LOCUS Inventors: Maurizio Ceppi (Issy-Les-Moulineaux, FR) Jennifer Abscheidt (Nogent Sur Marne, FR) Emmanuel Conseiller (Paris, FR) Etienne Rouleau (Paris, FR) Assignees: GENOMIC VISION |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100331 |