CN101680002A - 一组重组腺相关病毒载体及其构建方法与应用 - Google Patents
一组重组腺相关病毒载体及其构建方法与应用 Download PDFInfo
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Abstract
一组重组腺相关病毒载体(rAAV)及其构建方法与其应用。该rAAV是将腺相关病毒载体中的腺相关病毒结构基因替换为肿瘤抗原基因PSA、PSMA、CEA、CK19、Her－2/neu、LMP－1、BA46或AFP或其突变型基因得到。本发明的rAAV可将其携带的野生型或突变型的前列腺特异性抗原基因输送入单核细胞－巨噬细胞－树突状细胞系中，被用于刺激免疫系统的效应细胞。实验证明，被本发明的rAAV感染的DC所诱导的CTL在患者体内可有效地抑制恶性肿瘤细胞的生长或者杀灭肿瘤细胞。本发明的重组腺相关病毒载体或其相关产品可被用于制备治疗前列腺癌、上皮细胞恶性肿瘤、乳腺癌、结肠癌、胃癌、肺腺癌、肺癌、卵巢癌、鼻咽癌、宫颈癌、肺鳞癌、肝癌等的药物。
Description
PCT国内申请，说明书已公开。
Claims (1)
- PCT国内申请，权利要求书已公开。
Priority Applications (1)
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| CN200710097935 | 2007-04-23 | ||
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| CN2008800129496A CN101680002B (zh) | 2007-04-23 | 2008-04-23 | 一组重组腺相关病毒载体及其构建方法与应用 |
| PCT/CN2008/000835 WO2008128440A1 (fr) | 2007-04-23 | 2008-04-23 | Série de vecteurs recombinés dérivés du virus adéno-associé (raav), leurs procédés de construction et leurs utilisations |
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| CN2011101256327A Division CN102268454B (zh) | 2007-04-23 | 2008-04-23 | 一种psma重组腺相关病毒载体及其构建方法与应用 |
| CN2011101256030A Division CN102277384B (zh) | 2007-04-23 | 2008-04-23 | 一种psa重组腺相关病毒载体及其构建方法与应用 |
| CN2011101256435A Division CN102268455B (zh) | 2007-04-23 | 2008-04-23 | 一种cea重组腺相关病毒载体及其构建方法与应用 |
| CN2011101256651A Division CN102268453B (zh) | 2007-04-23 | 2008-04-23 | 一种lmp-1重组腺相关病毒载体及其构建方法与应用 |
| CN2011101256971A Division CN102268458B (zh) | 2007-04-23 | 2008-04-23 | 一种ba46重组腺相关病毒载体及其构建方法与应用 |
| CN201110125683XA Division CN102268457B (zh) | 2007-04-23 | 2008-04-23 | 一种afp重组腺相关病毒载体及其构建方法与应用 |
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| CN2011101256609A Active CN102268456B (zh) | 2007-04-23 | 2008-04-23 | 一种Her-2/neu重组腺相关病毒载体及其构建方法与应用 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US11761020B2 (en) | 2020-10-15 | 2023-09-19 | Aavocyte, Inc. | Recombinant adeno-associated virus vectors with CD14 promoter and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101775375B (zh) * | 2009-07-31 | 2013-04-10 | 华中科技大学同济医学院附属同济医院 | 制备含eb病毒潜伏膜蛋白1,2基因的重组腺相关病毒及其应用 |
| CN101638655B (zh) * | 2009-09-03 | 2011-05-04 | 杭州安倍生物科技有限公司 | 癌胚抗原阳性细胞靶向性基因表达元件cpe及其应用 |
| CN105316361B (zh) * | 2014-08-01 | 2017-11-28 | 广东拓谱康生物科技有限公司 | 携带人乳头瘤病毒16型突变型E7m58抗原基因的重组腺相关病毒载体及其构建方法与应用 |
| CN105177047B (zh) * | 2014-08-12 | 2018-02-02 | 广东拓谱康生物科技有限公司 | 携带人乳头瘤病毒16型突变型E7m94抗原基因的重组腺相关病毒载体及其构建方法与应用 |
| CN105018525B (zh) * | 2014-08-12 | 2017-06-13 | 广东拓谱康生物科技有限公司 | 携带人乳头瘤病毒16型突变型E7m91抗原基因的重组腺相关病毒载体及其构建方法与应用 |
| CN105177048B (zh) * | 2014-08-12 | 2018-02-02 | 广东拓谱康生物科技有限公司 | 携带人乳头瘤病毒16型多点突变型E7mm抗原基因的重组腺相关病毒载体及其构建方法与应用 |
| CN104830780A (zh) * | 2015-05-05 | 2015-08-12 | 杨光华 | 基于ba46抗原的dc细胞、靶向性免疫细胞群及其制备方法和用途 |
| CN104830782A (zh) * | 2015-05-05 | 2015-08-12 | 杨光华 | 基于ck19抗原的dc细胞、靶向性免疫细胞群及其制备方法和用途 |
| CN104830800A (zh) * | 2015-05-05 | 2015-08-12 | 杨光华 | 基于psma抗原的dc细胞、靶向性免疫细胞群及其制备方法和用途 |
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| US5173414A (en) * | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
| US6652850B1 (en) * | 1993-09-13 | 2003-11-25 | Aventis Pharmaceuticals Inc. | Adeno-associated viral liposomes and their use in transfecting dendritic cells to stimulate specific immunity |
| US6254862B1 (en) * | 1997-03-03 | 2001-07-03 | Calydon, Inc. | Adenovirus vectors specific for cells expressing alpha-fetoprotein and methods of use thereof |
| CN100402655C (zh) * | 2005-03-17 | 2008-07-16 | 中山大学中山医学院科技开发中心 | 人神经营养素-3受体基因重组腺病毒构建方法 |
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| US11761020B2 (en) | 2020-10-15 | 2023-09-19 | Aavocyte, Inc. | Recombinant adeno-associated virus vectors with CD14 promoter and use thereof |
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| CN102268458B (zh) | 2013-04-03 |
| CN101307328A (zh) | 2008-11-19 |
| CN102268455A (zh) | 2011-12-07 |
| CN101307326A (zh) | 2008-11-19 |
| CN101307327A (zh) | 2008-11-19 |
| CN102268456A (zh) | 2011-12-07 |
| CN102268457A (zh) | 2011-12-07 |
| CN101680002B (zh) | 2011-11-23 |
| CN101307325A (zh) | 2008-11-19 |
| CN101255441A (zh) | 2008-09-03 |
| CN102268455B (zh) | 2013-09-18 |
| CN102277384A (zh) | 2011-12-14 |
| CN101307330A (zh) | 2008-11-19 |
| CN102268457B (zh) | 2013-06-26 |
| CN102268456B (zh) | 2013-02-06 |
| CN101255442A (zh) | 2008-09-03 |
| CN102268453A (zh) | 2011-12-07 |
| CN102268458A (zh) | 2011-12-07 |
| WO2008128440A1 (fr) | 2008-10-30 |
| CN102268454A (zh) | 2011-12-07 |
| CN101307329A (zh) | 2008-11-19 |
| CN102268454B (zh) | 2013-07-24 |
| CN102268453B (zh) | 2013-04-03 |
| CN102277384B (zh) | 2013-06-26 |
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