CN101679990B - △8去饱和酶及其在制备多不饱和脂肪酸中的用途 - Google Patents

△8去饱和酶及其在制备多不饱和脂肪酸中的用途 Download PDF

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CN101679990B
CN101679990B CN2008800187631A CN200880018763A CN101679990B CN 101679990 B CN101679990 B CN 101679990B CN 2008800187631 A CN2008800187631 A CN 2008800187631A CN 200880018763 A CN200880018763 A CN 200880018763A CN 101679990 B CN101679990 B CN 101679990B
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seq
desaturase
acid
sequence
host cell
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CN101679990A (zh
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H·G·达穆德
Q·Q·朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
CN2008800187631A 2007-04-10 2008-04-10 △8去饱和酶及其在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN101679990B (zh)

Applications Claiming Priority (3)

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US91083107P 2007-04-10 2007-04-10
US60/910,831 2007-04-10
PCT/US2008/004700 WO2008124194A1 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids

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CN101679990A CN101679990A (zh) 2010-03-24
CN101679990B true CN101679990B (zh) 2013-06-19

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US (5) US8188338B2 (cg-RX-API-DMAC7.html)
EP (2) EP2121737B1 (cg-RX-API-DMAC7.html)
JP (1) JP5501952B2 (cg-RX-API-DMAC7.html)
CN (1) CN101679990B (cg-RX-API-DMAC7.html)
AU (2) AU2008237065A1 (cg-RX-API-DMAC7.html)
BR (1) BRPI0808222A2 (cg-RX-API-DMAC7.html)
CA (2) CA2681145C (cg-RX-API-DMAC7.html)
DK (1) DK2132317T3 (cg-RX-API-DMAC7.html)
WO (2) WO2008124806A2 (cg-RX-API-DMAC7.html)

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WO2011133610A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
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JP2013535988A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
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BR112016003225B1 (pt) 2013-08-16 2022-10-25 Pioneer Hi-Bred International, Inc. Polipeptídeo pip-47, polipeptídeo pip-47 quimérico, composição, proteína de fusão, método para controlar uma população de inseto-praga, método para inibir o crescimento ou matar um inseto-praga, construto de dna, polinucleotídeo isolado, cassete de expressão, método de obtenção de uma planta transgênica e método para controlar infestação de inseto
BR112016005543B1 (pt) 2013-09-13 2022-03-08 Pioneer Hi-Bred International, Inc Polipeptídeo pip-72 recombinante, construto de dna, método de obtenção de planta transgênica, célula hospedeira, composição, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto, método para controlar uma infestação de insetos em uma planta transgênica, método para identificar em uma amostra biológica uma sequência de nucleotídeos, método para identificar em uma amostra um polipeptídeo pip-72
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
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WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
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RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN107771181A (zh) 2015-06-16 2018-03-06 先锋国际良种公司 用以防治昆虫有害生物的组合物和方法
BR122023016256B8 (pt) 2015-08-06 2024-04-30 Du Pont Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo
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EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11008585B2 (en) 2016-05-04 2021-05-18 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3022858A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
EP3478052B1 (en) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
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WO2018084936A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110087481A (zh) 2016-12-15 2019-08-02 帝斯曼知识产权资产管理有限公司 包含硅酸盐和含具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的微生物细胞和/或植物细胞的共混物制剂
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CA2681145C (en) 2016-02-16
US20120196347A1 (en) 2012-08-02
CA2683497C (en) 2015-08-11
US8188338B2 (en) 2012-05-29
EP2121737A2 (en) 2009-11-25
BRPI0808222A2 (pt) 2014-07-08
EP2132317A1 (en) 2009-12-16
AU2008236595A1 (en) 2008-10-16
CN101679990A (zh) 2010-03-24
EP2121737B1 (en) 2015-05-27
US20080254521A1 (en) 2008-10-16
US20100267099A1 (en) 2010-10-21
US20080254192A1 (en) 2008-10-16
WO2008124194A1 (en) 2008-10-16
CA2681145A1 (en) 2008-10-16
JP5501952B2 (ja) 2014-05-28
AU2008237065A1 (en) 2008-10-16
US20100099900A1 (en) 2010-04-22
WO2008124806A3 (en) 2010-07-22
EP2132317B1 (en) 2013-02-27
JP2010523147A (ja) 2010-07-15
US8338152B2 (en) 2012-12-25
AU2008236595B2 (en) 2013-06-13
CA2683497A1 (en) 2008-10-16
WO2008124806A2 (en) 2008-10-16
DK2132317T3 (da) 2013-06-03
US7790156B2 (en) 2010-09-07
US8318463B2 (en) 2012-11-27

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