CN101658242A - Microbial Chinese medicinal herb fodder zymophyte agent, preparation method thereof and application thereof - Google Patents
Microbial Chinese medicinal herb fodder zymophyte agent, preparation method thereof and application thereof Download PDFInfo
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- CN101658242A CN101658242A CN200810146724A CN200810146724A CN101658242A CN 101658242 A CN101658242 A CN 101658242A CN 200810146724 A CN200810146724 A CN 200810146724A CN 200810146724 A CN200810146724 A CN 200810146724A CN 101658242 A CN101658242 A CN 101658242A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a fodder zymophyte agent, a preparation method thereof and application thereof, which belong to the field of livestock cultivation. The microbial Chinese medicinal herb fodderzymophyte agent is prepared from the following raw materials in portion by weight: 20 to 40 portions of rice water, 0.3 to 0.5 portion of brown sugar and 60 to 80 portions of Chinese medicinal herbs.Through the microbial Chinese medicinal herb fodder zymophyte agent, the straw 'lignin' can be thoroughly denatured; the substances such as nodes, wax coats and the like which are not easy to be digested in the straws can be degraded into small molecular substances which are easy to be absorbed by animals through the decomposition function of microbial colonies; and the crude protein of the strawsis improved to 9 to 13 percent, the digestion rate reaches over 75 to 85 percent, the crude protein can be well absorbed by the animals, the weight of the animals is increased quickly, the fodder conversion rate is high, the daily gain and the milk yield are improved obviously, the meat quality is good, and the mouth feel is good. Moreover, the fodder zymophyte agent can kill all impurity bacteria, parasitic ovum and harmful components in the raw materials, ensure safety and innocuousness after the animals eat the fodder zymophyte agent, improve the immunity of the animals, and reduce the occurrence of epidemic diseases.
Description
Technical field
The present invention relates to a kind of fodder zymophyte agent, relate in particular to microorganism Chinese herbal feed fermenting agent that the traditional Chinese herbal medicine of a kind of usefulness makes and preparation method thereof, the invention still further relates to this microorganism Chinese herbal feed fermenting agent various straws are fermented into application in the animal feed, belong to the livestock-raising field.
Background technology
Agricultural crop straw is considered as unconventional roughage by animal nutrition circle always, and this is because agricultural crop straw exists following shortcoming: 1. crude protein content is low, lacks necessary nutrient, bad; 2. the crude fiber content height wherein contains the difficult lignin that is digested by domestic animal; 3. NFE content height, main component is hemicellulose and polyuronide; 4. some stalk quality is thick and stiff, and palatability is poor, and directly the nutritive value as feed is very low so cause stalk, and digestibility only has 25-35%, and feed intake only is about 60% of a high quality forage; 5. the metabolizable energy utilization rate is relatively poor.
China's stalk crude fibre class resource is extremely abundant, and annual production reaches 5.7 hundred million tons, and quantity is equivalent to 50 times of the northern grassland amount of beating the grass in every year, accounts for the 20%--30% of whole world stalk total output.But stalk as the part of feed conversion also not enough total output 10%.
Fermented feed is to utilize growth and breeding and the metabolism of microorganism in feed, accumulate mesostates such as useful thalline, enzyme and pass through fermentation, the physicochemical character that changes feed improves nutritional labeling and digestibility, prolongs storage period, a kind of new technology that turns waste into wealth.
Summary of the invention
One of purpose of the present invention provides a kind of microorganism Chinese herbal feed fermenting agent.
Two of purpose of the present invention provides a kind of preparation method who prepares mentioned microorganism Chinese herbal feed fermenting agent.
Three of purpose of the present invention is the application with mentioned microorganism Chinese herbal feed fermenting agent, that is: with it as the microbial fermentation microbial inoculum, various straws are converted into the feed that livestock and poultry can absorb and utilize.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of microorganism Chinese herbal feed fermenting agent, mainly make by each raw material of following weight portion:
Rice thin rice gruel 20-40 part, brown sugar 0.3-0.5 part and Chinese herbal medicine 60-80 part;
Preferably, the weight portion of each raw material is: 30 parts of rice thin rice gruels, 0.4 part in brown sugar, 70 parts in Chinese herbal medicine;
Wherein, described Chinese herbal medicine is made up of each component of following weight fraction:
Medium-height grass 800-1200 part, Radix Astragali 125-180 part, genseng 40-60 part, safflower 20-30 part, Radix Glycyrrhizae 65-85 part, glossy ganoderma bacterium conidia powder 20-30 part, Cordyceps sinensis 40-60 part, Radix Angelicae Sinensis 40-60 part, fruit of Chinese magnoliavine 20-30 part, beautiful osmanthus 40-60 part, tamarind seed 40-60 part, Radix Isatidis 65-85 part, fruit of Chinese wolfberry 90-110 part, dried orange peel 20-30 part, fructus hordei germinatus 200-300 part of deer stomach the inside;
Preferably, the weight portion of each component is: 1000 parts of wild deer stomach medium-height grass, 150 parts of the Radixs Astragali, 50 parts of gensengs, 25 parts of safflowers, 75 parts in Radix Glycyrrhizae, 25 parts of glossy ganoderma bacterium conidia powder, 50 parts of Cordyceps sinensis, 50 parts of Radix Angelicae Sinensis, 25 parts in the fruit of Chinese magnoliavine, 50 parts in beautiful osmanthus, 50 parts of tamarind seeds, 75 parts of Radix Isatidis, 100 parts of the fruits of Chinese wolfberry, 25 parts of dried orange peels, 250 parts of fructus hordei germinatus;
In order to reach better effect, described rice thin rice gruel is preferably with the made thin rice gruel of old rice.
Wherein, the medium-height grass of described deer stomach the inside is meant the various food grass that do not digested as yet of deer stomach the inside, contains a large amount of deer gastric mucus in this food grass, the effect that has fermentation and increase energy, preferably, the medium-height grass of the described deer stomach the inside medium-height grass of wild deer stomach the inside preferably.
The Radix Astragali: contain sucrose, glucuronic acid, mucilaginous substance, several amino acid, bitter principle, choline, betaine, folic acid etc.Function: can increase albumen and albuminous total amount.Help gas, strengthen the bone, put on flesh and enrich blood.Prevention and treatment scrofula, goitre Ao, intestines wind, metrorrhagia, heatstroke etc.
Genseng: contain sugared consisting of in panaxoside, the personal saponin molecule: carbohydrate, VB1, VB2, nicotinic acid, pantothenic acid etc. such as glucose, fructose, maltose, sucrose, several panose, pectin.Function: can strengthen the ability of body to the defence capability of various destructive stimuluses, the metabolic capability that strengthens animal, the enhancing circulatory system.And can increase food proteins and crude fibre.Increase the heat and the energy of feed, increase the adaptive capacity of the immunity of physique.
Safflower: it is about 2% to contain crocin, is crocetin and two molecule gentiobiose be combined into esters, contains safflower dimethyl ester, safflower again and covers plain about 2%, volatile oil 0.4-1.3% (being mainly safranal) with mat.Contain abundant VB2 again.Bulb contains glucose, amino acid, saponin etc.Function: promoting blood circulation and removing blood stasis, diffusing Yu Kaijie.Promote that feed ferments fast.Flavouring, enhancing palatability.
Radix Glycyrrhizae: contain triterpenoid saponin oxalic acid, promptly glycyrrhizin is the glucosulfone aldehydic acid glucoside of enoxolone, is the sweet taste composition of Radix Glycyrrhizae.Also contain biosone's (18a-enoxolone) in the hydrolysate of licorice.By 18 kinds of sample analysis results: hydrotrope 18.7-40.54%, glycyrrhizic acid 3.63-13.06%, reduced sugar 3.38-13.67%, starch and colloid 2.04-6.32%, moisture content 6.04-8.44%, ash content 3.35-6.68%.Function: and middle emergency, speed fermentation, detoxicating, killing bacteria, all medicines of adjusting.
Glossy ganoderma bacterium (conidia powder): purple sesame contains ergosterol, organic acid (ricinic acid, fumaric acid etc.), Glucosamine, polysaccharide, resin, sweet mellow wine etc.Function: control consumptive disease, antitussive and antiasthmatic, aid digestion, enhancing palatability.Consumption proportion half two.
Cordyceps sinensis: water content 10.84%, fat 8.4%, thick protein 25.32%, crude fibre 18.53%, carbohydrate 28.90%, ash content 4.10%, fat contain about 7%, the VB130.29 microgram/100 grams of saturated fatty acid 13.00%, unrighted acid 82.20%, the acid of bat moth.Bat moth element is a kind of light yellow crystalline powder, can suppress the growth of streptococcus, Actinobacillus mallei, bacillus anthracis, pig bacillus bipolaris septicus and staphylococcus in vitro.Function: qi-restoratives damage, beneficial vital essence, relieving cough and reducing sputum, antibiotic desinsection have certain inhibitory action to pneumococcus and some pathogenic fungus.
Radix Angelicae Sinensis: contain volatile oil 0.2-0.7%, but the composition complexity of volatile oil singly is that the low boiling part just has kind more than 10, and multiple hydro carbons is wherein arranged, and comprises three kinds of terpenes.Other contains with sucrose 40%, VB12 (0.25-40 microgram/100 grams), VA class material.Contain palmitic acid, stearic acid, nutmeg and unsaturated oleic acid, linoleic acid in the saponification part.Contain cupreol in the not saponification composition.Function: enrich blood and close blood, menstruction regulating and pain relieving, moisturize laxation, sterilizing.Strengthen food energy.
The fruit of Chinese magnoliavine: fruit contains and contains citric acid 12%, malic acid 10% and a small amount of tartaric acid in volatile oil 3%, the dry fruit, also contains monosaccharide, resinae etc.The fatty oil of seed is about 33%, and the effective ingredient that its non-soap partly contains tonic is profound and plain about 0.12%, also contain r-, c-, p-, profound and plain, deoxidation is profound and plain and profound and pure.Also contain volatile oil 1.6%, Main Ingredients and Appearance is a citral.Contain chlorophyll, sterol, VC, E, resin, tannin and a small amount of carbohydrate in addition.Function: it is disorderly to normal value to regulate animal nerve.And the acid-base value that can regulate food, strengthen palatability.
Beautiful osmanthus: beautiful osmanthus is subtropical zone Lauraceae aiphyllium, the bark taupe, and sprout has four ribs, leaf alternate or to life, keratin, oblong, the short point of leaf front end, base portion (wooden contract) shape, above green glossy tool petiole. the panicle armpit is given birth to. the berry atropurpureus, black ovalize, the shallow shape hypocarp of tool. June at florescence, really October phase to next year 2 was to＜Compendium of Materia Medica in March to August〉record male osmanthus, bacterium osmanthus, claim meat Guo in the osmanthus heart and the hospital, beautiful osmanthus, purple osmanthus, the osmanthus heart is a thing and different name all.Nowadays, beautiful osmanthus mainly is distributed in China, Vietnam, Sri Lanka, Indonesia, Burma, states such as Cambodia. and China Guangxi, Guangdong, Yunnan are main producing region, beautiful osmanthus. mostly be artificial growth.Contain volatile oil (claiming beautiful cassia oil) 1-2%, Main Ingredients and Appearance is beautiful cinnaldehydrum 75-90%, and contains the beautiful cassia bark fat of small amount of acetic acid, acetate phenylpropyl alcohol fat, mucus, tannin etc.Function: complement sun, warming spleen and stomach, removing internal cold, promoting blood circulation.Exceptional function: kill the vegetation poison.
Tamarind seed: contain tartaric acid, citric acid, smoked plum acid, flavonoids, lactone, sugar and glucoside.Contain citric acid, malic acid, smoked plum acid in the wild fruit.Tannin, saponin, fructose, vitamin C, protein and fat etc.Function: long-pending, diffusing extravasated blood, expelling tenia help digestion.Shigella dysenteriae and Pseudomonas aeruginosa there is the obvious suppression effect.Can also strengthen the palatability of fragrance and feed.
Radix Isatidis: contain indigo glucoside, cupreol, isatin, the brilliant second, third of indigo-blue root knot, fourth.Contain vegetable protein, resin-like thing, carbohydrate etc. again.Amino acid has arginine, proline, glutamic acid, tyrosine, r aminobutyric acid, propylhomoserin and leucine in the root.Contain sinigrin again.Also have anti-leather blue antibacterial substance and kinetin for positive and negative bacteria.Function: the plant poison that disappears, sterilization, and can improve protein content and energy in the feed.
The fruit of Chinese wolfberry: contain carrotene 3.39mg%, thiamine 0.23mg%, riboflavin 0.33mg%, nicotinic acid 1.7mg%, ascorbic acid 3mg%.Also contain cupreol, linoleic acid etc.Function: promote the generation and the growth of lactein, and can produce the comprehensive all medicines of acid.It is the first-selection of the feed processing of food processing.
Dried orange peel: contain volatile oil, be mainly citric acid, also have hesperidine, carrotene, VC150mg%, VB176mg%, pectin, glycosides displayed etc.Function: regulate the flow of vital energy transfer in, gentle, longue meat helps digestion.Consumption proportion half two.
Fructus hordei germinatus: contain allantoin.That function: Li Shui lets out is wet, promote the production of body fluid sliding dry, change that paddy helps digestion, expel flatulence and relieve chest stuffiness, help to promote the effect of crude protein.
A kind of method for preparing mentioned microorganism Chinese herbal feed fermenting agent comprises:
1, takes by weighing each raw material by described weight proportion;
2, be contained in the mud jar after each raw material is mixed, charge weight is no more than jar 1/3rd, is embedded in depths under the in the shade hillside fields after the altar mouth is obturaged, and takes out in 8-10 month, takes out the bacterium network that covers with white in the jar, gets original zymophyte;
3, the female kind of preparation second phase: the preparation-obtained original zymophyte of said method is carried out the inclined-plane with potato dextrose agar cultivate, after cultivating 35 days under 23-30 ℃ the temperature conditions, obtain female kind of second phase;
4, the preparation of cultigen:
(1) preparation of culture medium: take by weighing raw material: 1.2 jin of the powder of straw that nothing is gone mouldy, 0.3 jin of 10% white sugar water, 0.2 jin of bean cake powder by following weight; Above-mentioned raw materials is encased in autoclave sterilization in the aluminium box;
(2) in culture medium after sterilization, insert female kind of second phase (calculate by volume, inoculum concentration is about 2-10%), under 23-30 ℃ temperature conditions, be cultured to white hypha and cover with the aluminium box, promptly.
In the said method, preferred in the step 2, seal tight with pig urine the altar mouth; Be embedded in following 50 centimeters depths, in the shade hillside fields after the altar mouth obturaged;
The compound method of the potato dextrose agar described in the step 3 is as follows:
Potato 200g, agar 18-20g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, VB11 0mg, water 1000ml; Autoclave sterilization, promptly.
The resulting cultigen of above-mentioned preparation method can be inoculated into and carry out anaerobic fermentation in the stalk, wherein, but 1 ton of stalk of each box cultigen anaerobic fermentation; Fermentation temperature is 8-30 ℃, and fermentation time can change according to the height of fermentation temperature, the fermentation temperature height, and then fermentation time will be lacked, and is low as fermentation temperature, and then fermentation time wants corresponding longer, and preferred, described fermentation time is 7-10 days.
Beneficial effect of the present invention
1, nutritional labeling height: with the stalk animal feed of microorganism Chinese herbal medicine fermenting agent fermentation of the present invention, need not pass through high temperature, high pressure, just can make thoroughly sex change of stalk " lignin ", make its crude protein bring up to 9%-13%, digestibility reaches more than the 75%-85%, so animal food back good absorbing, fast gaining.
2, conversion ratio height: can make with the straw feed of microorganism Chinese herbal medicine zymophyte fermentation to be difficult for the joint that digested and wax coat etc. in the stalk by the decomposition of suitable microorganism species is arranged, be degraded into the small-molecule substance that is very easily absorbed by animal, ight soil is few behind the animal edible, the feed conversion rate height, the daily gain and the output of milk are significantly improved, meat is good, and mouthfeel is good.
3, eliminate stink: pollution-free to environment and air.Clean hygiene in the pen for animal, the four seasons do not have the foreign odor flavor.Living environment of animal and people's working environment there is great improvement.
4, safety and sanitation: by microorganism Chinese herbal medicine flora strain fermentation, make assorted bacterium all in the raw material, parasitic ovum and harmful components are killed; decompose and performance, guarantee that animal is safe and harmless after eating, and protective effect is arranged; be animal physique stalwartness, reduce the disease epidemic disease and take place.
5, easy to use: because volume is little, per 1.5 kilograms of microorganism Chinese herbal medicine fermenting agents of the present invention one ton of stalk that just can ferment can be used feed.And the maintenance phase can never degenerate up to 12 months.This has created the condition that cause is cultured in good development just for large-scale cultivation field and individual raiser.
6, raw material sources are extensive: all nontoxic, do not have that the various stalks go mouldy and various careless class, melon are climing, melon shell etc., behind microorganism Chinese herbal medicine flora strain fermentation of the present invention, all can be used as feed (wherein, only stalk or various careless class, melon moisture climing, the melon shell need be controlled at 25%-30%, just can ferment, and obtain nutritious feed) with microorganism Chinese herbal medicine flora bacterial classification of the present invention.
7, stalk grainization: after testing, after adopting microorganism Chinese herbal medicine flora strain fermentation maize straw of the present invention, can significantly improve use value, the corn composition of 34%-35% of purifying out behind the maize straw process microorganism Chinese herbal medicine bacteria fermentation, and contain the 9%-13% crude protein.
8, the object of feeding is extensive: it is edible to be suitable for all kinds of livestock and poultry animals such as horse, ox, sheep, goose, pig, dog, rabbit, chicken, duck, ermine, fox, racoon dog through the prepared feed of the inventive method.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
Embodiment 1
1, takes by weighing each raw material (unit: kg): rice thin rice gruel 40, brown sugar 0.5, Chinese herbal medicine 80 by following weight; Wherein, described Chinese herbal medicine is made up of each component of following weight portion:
85 parts in 30 parts of 60 parts of 180 parts of 1200 parts of medium-height grass, the Radixs Astragali, gensengs, safflowers, Radix Glycyrrhizae, 30 parts of glossy ganoderma bacterium conidia powder, 60 parts of Cordyceps sinensis, 60 parts of Radix Angelicae Sinensis, 30 parts in the fruit of Chinese magnoliavine, 60 parts in beautiful osmanthus, 60 parts of tamarind seeds, 85 parts of Radix Isatidis, 110 parts of the fruits of Chinese wolfberry, 30 parts of dried orange peels, 300 parts of the fructus hordei germinatus of deer stomach the inside;
2, be contained in the mud jar after each raw material is mixed, charge weight is no more than jar 1/3rd, is embedded in depths under the in the shade hillside fields after the altar mouth is obturaged, and takes out in 8-10 month, takes out the bacterium network that covers with white in the jar, gets original zymophyte;
3, the female kind of preparation second phase: the preparation-obtained original zymophyte of said method is carried out the inclined-plane with potato dextrose agar cultivate, after cultivating 35 days under 23-30 ℃ the temperature conditions, obtain female kind of second phase;
The preparation of potato dextrose agar: potato 200g, agar 18-20g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, Cobastab
110mg, water 1000ml; Autoclave sterilization, promptly.
4, the preparation of cultigen:
(1) preparation of culture medium: take by weighing raw material: 1.2 jin of the powder of straw that nothing is gone mouldy, 0.3 jin of 10% white sugar water, 0.2 jin of bean cake powder by following weight; Above-mentioned raw materials is encased in autoclave sterilization in the aluminium box;
(2) in culture medium after sterilization, insert female kind of second phase, under 23-30 ℃ temperature conditions, be cultured to white hypha and cover with the aluminium box, promptly.
Embodiment 2
1, takes by weighing each raw material (unit: kg): rice thin rice gruel 20, brown sugar 0.3, Chinese herbal medicine 60 by following weight;
Wherein, described Chinese herbal medicine is made up of each component of following weight portion:
65 parts in 20 parts of 40 parts of 125 parts of 800 parts of medium-height grass, the Radixs Astragali, gensengs, safflowers, Radix Glycyrrhizae, 20 parts of glossy ganoderma bacterium conidia powder, 40 parts of Cordyceps sinensis, 40 parts of Radix Angelicae Sinensis, 20 parts in the fruit of Chinese magnoliavine, 40 parts in beautiful osmanthus, 40 parts of tamarind seeds, 65 parts of Radix Isatidis, 90 parts of the fruits of Chinese wolfberry, 20 parts of dried orange peels, 200 parts of the fructus hordei germinatus of deer stomach the inside;
2, be contained in the mud jar after each raw material is mixed, charge weight is no more than jar 1/3rd, is embedded in depths under the in the shade hillside fields after the altar mouth is obturaged, and takes out in 8-10 month, takes out the bacterium network that covers with white in the jar, gets original zymophyte;
3, the female kind of preparation second phase: the preparation-obtained original zymophyte of said method is carried out the inclined-plane with potato dextrose agar cultivate, after cultivating 35 days under 23-30 ℃ the temperature conditions, obtain female kind of second phase;
The preparation of potato dextrose agar: potato 200g, agar 18-20g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, Cobastab
110mg, water 1000ml; Autoclave sterilization, promptly.
4, the preparation of cultigen:
(1) preparation of culture medium: take by weighing raw material: 1.2 jin of the powder of straw that nothing is gone mouldy, 0.3 jin of 10% white sugar water, 0.2 jin of bean cake powder by following weight; Above-mentioned raw materials is encased in autoclave sterilization in the aluminium box;
(2) in culture medium after sterilization, insert female kind of second phase, under 23-30 ℃ temperature conditions, be cultured to white hypha and cover with the aluminium box, promptly.
Embodiment 3
1, takes by weighing each raw material (unit: kg): rice thin rice gruel 30, brown sugar 0.4, Chinese herbal medicine 70 by following weight;
Wherein, described Chinese herbal medicine is made up of each component of following weight portion: 1000 parts of wild deer stomach medium-height grass, 150 parts of the Radixs Astragali, 50 parts of gensengs, 25 parts of safflowers, 75 parts in Radix Glycyrrhizae, 25 parts of glossy ganoderma bacterium conidia powder, 50 parts of Cordyceps sinensis, 50 parts of Radix Angelicae Sinensis, 25 parts in the fruit of Chinese magnoliavine, 50 parts in beautiful osmanthus, 50 parts of tamarind seeds, 75 parts of Radix Isatidis, 100 parts of the fruits of Chinese wolfberry, 25 parts of dried orange peels, 250 parts of fructus hordei germinatus;
2, be contained in the mud jar after each raw material is mixed, charge weight is no more than jar 1/3rd, and the altar mouth is sealed with pig urine and is embedded in following 50 centimeters depths, in the shade hillside fields after tight, takes out in 8-10 month, takes out the bacterium network that covers with white in the jar, gets original zymophyte;
3, the female kind of preparation second phase: the preparation-obtained original zymophyte of said method is carried out the inclined-plane with potato dextrose agar cultivate, after cultivating 35 days under 23-30 ℃ the temperature conditions, obtain female kind of second phase;
The preparation of potato dextrose agar: potato 200g, agar 18-20g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, Cobastab
110mg, water 1000ml; Autoclave sterilization, promptly.
4, the preparation of cultigen:
(1) preparation of culture medium: take by weighing raw material: 1.2 jin of the powder of straw that nothing is gone mouldy, 0.3 jin of 10% white sugar water, 0.2 jin of bean cake powder by following weight; Above-mentioned raw materials is encased in autoclave sterilization in the aluminium box;
(2) in culture medium after sterilization, insert female kind of second phase, under 23-30 ℃ temperature conditions, be cultured to white hypha and cover with the aluminium box, promptly.
The application test of test example 1 microorganism Chinese herbal feed fermenting agent of the present invention
1, for the prepared fermenting agent of test agent: embodiment of the invention 1-3;
2, test method: will be inoculated in the maize straw of chopping for test agent, one box cultigen (about 1.5kg), the 1 ton of maize straw that can ferment is controlled at 25-30% with the moisture of maize straw, ferments under the condition of anaerobism, fermentation temperature is 8-30 ℃, and fermentation time is 7-10 days;
After testing, the content of the crude protein in the maize straw is by fermentation brought up to 9-13%, can compare to have had before the fermentation for the small-molecule substance (for example: amino acid, polysaccharose substance, mineral element, vitamin etc.) that livestock and poultry absorbed and utilized and significantly improve, and certain paste fragrance has been arranged, sweeter, the good palatability of mouthfeel.
Claims (10)
1, a kind of microorganism Chinese herbal feed fermenting agent is mainly made by each raw material of following weight portion: rice thin rice gruel 20-40 part, brown sugar 0.3-0.5 part and Chinese herbal medicine 60-80 part.
2, according to the microorganism Chinese herbal feed fermenting agent of claim 1, it is characterized in that the weight portion of each raw material is: 30 parts of rice thin rice gruels, 0.4 part in brown sugar, 70 parts in Chinese herbal medicine.
3, according to claim 1 or 2 described microorganism Chinese herbal feed fermenting agents, it is characterized in that described Chinese herbal medicine is made up of each component of following weight fraction: medium-height grass 800-1200 part, Radix Astragali 125-180 part, genseng 40-60 part, safflower 20-30 part, Radix Glycyrrhizae 65-85 part, glossy ganoderma bacterium conidia powder 20-30 part, Cordyceps sinensis 40-60 part, Radix Angelicae Sinensis 40-60 part, fruit of Chinese magnoliavine 20-30 part, beautiful osmanthus 40-60 part, tamarind seed 40-60 part, Radix Isatidis 65-85 part, fruit of Chinese wolfberry 90-110 part, dried orange peel 20-30 part and fructus hordei germinatus 200-300 part of deer stomach the inside.
4, according to the described microorganism Chinese herbal feed of claim 3 fermenting agent, it is characterized in that described Chinese herbal medicine is made up of each component of following weight fraction: 1000 parts of deer stomach medium-height grass, 150 parts of the Radixs Astragali, 50 parts of gensengs, 25 parts of safflowers, 75 parts in Radix Glycyrrhizae, 25 parts of glossy ganoderma bacterium conidia powder, 50 parts of Cordyceps sinensis, 50 parts of Radix Angelicae Sinensis, 25 parts in the fruit of Chinese magnoliavine, 50 parts in beautiful osmanthus, 50 parts of tamarind seeds, 75 parts of Radix Isatidis, 100 parts of the fruits of Chinese wolfberry, 25 parts of dried orange peels, 250 parts of fructus hordei germinatus;
According to claim 1 or 2 described microorganism Chinese herbal feed fermenting agents, it is characterized in that 5, described rice thin rice gruel is for using the made thin rice gruel of old rice.
6, a kind of method for preparing claim 1 or 2 described microorganism Chinese herbal feed fermenting agents comprises:
(1), takes by weighing each raw material by described weight proportion;
(2), prepare original zymophyte: be contained in the mud jar after each raw material is mixed, charge weight is no more than jar 1/3rd, is embedded in depths under the in the shade hillside fields after the altar mouth is obturaged, and takes out in 8-10 month, take out the bacterium network that covers with white in the jar, get original zymophyte;
(3), the female kind of preparation second phase: the preparation-obtained original zymophyte of step (2) is carried out the inclined-plane with potato dextrose agar cultivate, after cultivating 35 days under 23-30 ℃ the temperature conditions, obtain female kind of second phase;
(4), preparation cultigen:
(a) preparation of culture medium: take by weighing raw material: 1.2 jin of the powder of straw that nothing is gone mouldy, 0.3 jin of 10% white sugar water, 0.2 jin of bean cake powder by following weight; Above-mentioned raw materials is encased in autoclave sterilization in the aluminium box;
(b) in culture medium after sterilization, insert female kind of second phase, under 23-30 ℃ temperature conditions, be cultured to white hypha and cover with the aluminium box, promptly.
7, in accordance with the method for claim 6, it is characterized in that: in the step (2), seal tight with pig urine the altar mouth.
8, in accordance with the method for claim 6, it is characterized in that: in the step (2), be embedded in following 50 centimeters depths, in the shade hillside fields after Jiang Tankou obturages.
9, in accordance with the method for claim 6, it is characterized in that: the compound method of the potato dextrose agar described in the step 3 is as follows: potato 200g, agar 18-20g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, Cobastab
110mg, water 1000ml; Autoclave sterilization, promptly.
10, claim 1 or the 2 described microorganism Chinese herbal feed fermenting agents application in the preparation animal and fowl fodder, comprise: with claim 1 or 2 described microorganism Chinese herbal feed fermenting agents be inoculated into stalk, careless class, melon is climing or the melon shell in, the control water content is fermented under the condition of anaerobism at 25-30%.
Priority Applications (1)
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| CN102178042A (en) * | 2011-05-04 | 2011-09-14 | 李国丽 | Feed additive for feeding |
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| CN1565228A (en) * | 2003-07-03 | 2005-01-19 | 延边高丽家庭生活服务咨询有限公司 | Straw fodder leaven |
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| CN102334600B (en) * | 2011-10-31 | 2012-12-26 | 李耀曾 | Fermented feed and preparation method thereof |
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| CN102630814B (en) * | 2012-05-14 | 2013-05-08 | 魏县鸿健养殖有限公司 | Feed for livestock and poultry |
| CN103283966A (en) * | 2013-06-08 | 2013-09-11 | 金华市金帆饲料有限公司 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of wheat straws and palm cakes |
| CN103283966B (en) * | 2013-06-08 | 2015-03-18 | 金华市金帆饲料有限公司 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of wheat straws and palm cakes |
| CN104522313A (en) * | 2014-11-25 | 2015-04-22 | 合肥迈可罗生物工程有限公司 | Application of cordyceps strain fermentation product to reduce somatic cell count in cow's milk |
| CN104522313B (en) * | 2014-11-25 | 2016-02-24 | 合肥迈可罗生物工程有限公司 | Cordyceps strain fermentation thing is reducing the application in Methods of Milk Somatic Cell Images quantity |
| CN104472949A (en) * | 2014-12-18 | 2015-04-01 | 韩褆 | Energy-saving and environment-friendly microbial fermentation feed and preparation method thereof |
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Inventor after: Wang Houguo Inventor after: Han Xuan Inventor before: Wang Houguo |
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Effective date of registration: 20180321 Address after: 110042 Baita Road, Dadong District, Shenyang, Liaoning Province, No. 53-1 Patentee after: Han Xuan Address before: 151814 former Siping mountain village of Zhongxing village, Xinlong Town, Bayan County, Heilongjiang Province Patentee before: Wang Houguo |