CN101658177A - Complex bactericidal composition containing cyazofamid - Google Patents
Complex bactericidal composition containing cyazofamid Download PDFInfo
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- CN101658177A CN101658177A CN200910161260A CN200910161260A CN101658177A CN 101658177 A CN101658177 A CN 101658177A CN 200910161260 A CN200910161260 A CN 200910161260A CN 200910161260 A CN200910161260 A CN 200910161260A CN 101658177 A CN101658177 A CN 101658177A
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- China
- Prior art keywords
- cyanogen
- azoles
- frost azoles
- cyanogen frost
- metalaxyl
- Prior art date
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Links
- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 17
- 239000005754 Cyazofamid Substances 0.000 title abstract description 3
- YXKMMRDKEKCERS-UHFFFAOYSA-N cyazofamid Chemical compound CN(C)S(=O)(=O)N1C(C#N)=NC(Cl)=C1C1=CC=C(C)C=C1 YXKMMRDKEKCERS-UHFFFAOYSA-N 0.000 title abstract description 3
- 239000005807 Metalaxyl Substances 0.000 claims abstract description 30
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 claims abstract description 30
- CJPQIRJHIZUAQP-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(phenylacetyl)alaninate Chemical compound CC=1C=CC=C(C)C=1N(C(C)C(=O)OC)C(=O)CC1=CC=CC=C1 CJPQIRJHIZUAQP-UHFFFAOYSA-N 0.000 claims abstract description 30
- CIEXPHRYOLIQQD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-2-furoylalaninate Chemical compound CC=1C=CC=C(C)C=1N(C(C)C(=O)OC)C(=O)C1=CC=CO1 CIEXPHRYOLIQQD-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000005808 Metalaxyl-M Substances 0.000 claims abstract description 29
- ZQEIXNIJLIKNTD-GFCCVEGCSA-N metalaxyl-M Chemical compound COCC(=O)N([C@H](C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-GFCCVEGCSA-N 0.000 claims abstract description 29
- 201000010099 disease Diseases 0.000 claims abstract description 22
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 22
- 241000233654 Oomycetes Species 0.000 claims abstract description 11
- 239000004480 active ingredient Substances 0.000 claims abstract description 11
- 235000013311 vegetables Nutrition 0.000 claims abstract description 4
- 229920000742 Cotton Polymers 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 3
- 235000021307 Triticum Nutrition 0.000 claims abstract description 3
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 3
- 235000009566 rice Nutrition 0.000 claims abstract description 3
- 241000209140 Triticum Species 0.000 claims abstract 2
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 claims description 358
- 150000003851 azoles Chemical class 0.000 claims description 153
- JHXVRRJXCDAINK-UHFFFAOYSA-N NC(=O)N.N#CC#N Chemical compound NC(=O)N.N#CC#N JHXVRRJXCDAINK-UHFFFAOYSA-N 0.000 claims description 36
- 238000009736 wetting Methods 0.000 claims description 27
- 239000000843 powder Substances 0.000 claims description 24
- 241000233679 Peronosporaceae Species 0.000 claims description 23
- 239000000375 suspending agent Substances 0.000 claims description 21
- 239000004562 water dispersible granule Substances 0.000 claims description 16
- 239000000839 emulsion Substances 0.000 claims description 7
- 239000004530 micro-emulsion Substances 0.000 claims description 7
- -1 Evil frost Chemical compound 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 230000002195 synergetic effect Effects 0.000 abstract description 31
- 239000005821 Propamocarb Substances 0.000 abstract description 26
- WZZLDXDUQPOXNW-UHFFFAOYSA-N propamocarb Chemical compound CCCOC(=O)NCCCN(C)C WZZLDXDUQPOXNW-UHFFFAOYSA-N 0.000 abstract description 26
- XERJKGMBORTKEO-VZUCSPMQSA-N (1e)-2-(ethylcarbamoylamino)-n-methoxy-2-oxoethanimidoyl cyanide Chemical compound CCNC(=O)NC(=O)C(\C#N)=N\OC XERJKGMBORTKEO-VZUCSPMQSA-N 0.000 abstract 1
- 239000005734 Benalaxyl Substances 0.000 abstract 1
- 240000002791 Brassica napus Species 0.000 abstract 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 abstract 1
- 239000005756 Cymoxanil Substances 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 abstract 1
- UWVQIROCRJWDKL-UHFFFAOYSA-N oxadixyl Chemical compound CC=1C=CC=C(C)C=1N(C(=O)COC)N1CCOC1=O UWVQIROCRJWDKL-UHFFFAOYSA-N 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 62
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 28
- 238000012360 testing method Methods 0.000 description 25
- 239000002131 composite material Substances 0.000 description 23
- 230000003449 preventive effect Effects 0.000 description 21
- 240000008067 Cucumis sativus Species 0.000 description 17
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 17
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 16
- 240000003768 Solanum lycopersicum Species 0.000 description 16
- 238000000034 method Methods 0.000 description 15
- 230000001988 toxicity Effects 0.000 description 15
- 231100000419 toxicity Toxicity 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 14
- 239000002270 dispersing agent Substances 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000000945 filler Substances 0.000 description 10
- 239000003995 emulsifying agent Substances 0.000 description 9
- 238000011835 investigation Methods 0.000 description 9
- 230000002265 prevention Effects 0.000 description 9
- 239000002904 solvent Substances 0.000 description 9
- 238000010998 test method Methods 0.000 description 9
- 238000005507 spraying Methods 0.000 description 7
- 239000000080 wetting agent Substances 0.000 description 7
- 206010039509 Scab Diseases 0.000 description 6
- 239000003905 agrochemical Substances 0.000 description 6
- 230000003902 lesion Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000005995 Aluminium silicate Substances 0.000 description 5
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 5
- 235000012211 aluminium silicate Nutrition 0.000 description 5
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000009313 farming Methods 0.000 description 5
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 240000008415 Lactuca sativa Species 0.000 description 4
- 235000003228 Lactuca sativa Nutrition 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 239000012752 auxiliary agent Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 description 4
- 231100000820 toxicity test Toxicity 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 3
- 230000000857 drug effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 244000302512 Momordica charantia Species 0.000 description 2
- 235000009811 Momordica charantia Nutrition 0.000 description 2
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 2
- 235000018365 Momordica dioica Nutrition 0.000 description 2
- 244000183278 Nephelium litchi Species 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 241000233614 Phytophthora Species 0.000 description 2
- 241000233622 Phytophthora infestans Species 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000006184 cosolvent Substances 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 102100025287 Cytochrome b Human genes 0.000 description 1
- 108010075028 Cytochromes b Proteins 0.000 description 1
- 241001281805 Pseudoperonospora cubensis Species 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- XUZHLZDRCCUWEV-UHFFFAOYSA-N formaldehyde;methyl naphthalene-1-sulfonate Chemical compound O=C.C1=CC=C2C(S(=O)(=O)OC)=CC=CC2=C1 XUZHLZDRCCUWEV-UHFFFAOYSA-N 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a bactericidal composition, the active ingredients are cyazofamid (A) and one of metalaxyl, metalaxyl-M, oxadixyl, benalaxyl, furalaxyl, cymoxanil or propamocarb (B), and theweight ratio of A to B is 50:1-1:50. The complex of A and B realizes the obvious synergistic effect, and the bactericidal composition is used for the disease control of the crops of fruit trees, flowers, cotton, wheat, rice, rapeseed, vegetables and the like, especially the diseases of oomycetes.
Description
Technical field
The present invention relates to a kind of bactericidal composition, especially a kind of combination sterilization composition that contains cyanogen frost azoles.
Background technology
In recent years, because single drug and other unscientific application methods have caused many diseases that a lot of agricultural chemicals of current use have been produced resistance, become a great problem of chemical control.Because resistance strengthens, the frequent dispenser of peasant, the corresponding increase of dosage with the medication number of times, thus cause peasant burden to increase the weight of and increasing environmental pollution.Performance is particularly outstanding in the disease chemical control that Oomycete causes.Because in the control of oomycetes diseases such as downy mildew, selectable medicament is also few, single drug and repeated drug taking phenomenon are quite obvious.Therefore, the bactericidal composition of urgent need synergy addresses this problem.
The English common name Cyazofamid of cyanogen frost azoles, chemical name 4-chloro-2-cyano group-5-p-methylphenyl-imidazoles-1-N, N-dimethyl methyl acid amides, a kind of mitochondrial respiratory inhibitor by Japanese Ishihara Sangyo Kaisha, Ltd. Development and Production acts on the Qi site among the cytochrome b cl.It is active that cyanogen frost azoles has good protection, and the lasting period is long, and resistance of rainwater washing against, also has certain interior suction and therapeutic activity.Cyanogen frost azoles all has activity to all vegetative stages of oomycetes.If can obtain some good synergistic formulas with some other composite reagent, then can reduce the usage amount of agricultural chemicals greatly, and can delay the speed that cyanogen frost azoles produces resistance to a certain extent.
Summary of the invention
The objective of the invention is to: the safety, the Synergistic bactericidal composition that contain cyanogen frost azoles that use on a kind of suitable agricultural, the oomycetes disease had outstanding control efficiency are provided.
For solving the problems of the technologies described above, the inventor is by a large amount of biologicall test screenings, a kind of (B) is composite with certain proportion in unexpected discovery cyanogen frost azoles (A) and metalaxyl, the spirit of Metalaxyl-M, Evil frost, M 9834, furalaxyl, white urea cyanogen or the Propamocarb, and the plant Oomycete disease is had significant synergies.The spirit of metalaxyl, Metalaxyl-M, Evil frost, Propamocarb, M 9834, furalaxyl, these several medicaments of white urea cyanogen all are the medicaments commonly used of preventing and treating the plant disease that oomycetes causes, because single drug, oomycetes diseases such as downy mildew can not be ignored to their pesticide resistance problem.Their mechanism of action is different with cyanogen frost azoles, action site is different may be its composite in twos reason that causes synergy.
On the basis of above-mentioned discovery, through composition is carried out symphyogenetic quantitative analysis, formed technical scheme of the present invention, promptly be a kind of active ingredient, be another active ingredient with (B) with cyanogen frost azoles (A), described (B) is a kind of in metalaxyl, the spirit of Metalaxyl-M, Evil frost, M 9834, furalaxyl, white urea cyanogen or the Propamocarb, (A) be 50 with (B) mass ratio: 1-1: 50, ratio is 10 preferably: 1-1: 20.The present composition can be prepared into known method and be fit to the formulation that agricultural is used, and as suspending agent, missible oil, aqueous emulsion, microemulsion, wetting powder and water dispersible granules, the total content of active ingredient is 5%-80% in the preparation.
The adjuvant that uses in the composition of the present invention comprises that dispersant, wetting agent, carrier etc. and other are of value to the known substance that active ingredient is stablized and drug effect is brought into play in storing and using, all be the various compositions of using or allow use in the pesticidal preparations always, be not particularly limited, concrete composition and consumption require to determine by simple experiment according to prescription.
Composition described in the invention can the finished product preparation form provide, and promptly each material mixes in the composition, and the composition of composition also can provide with single agent form, directly directly mixes in bucket or jar before the use, is diluted to required concentration then.
Bactericidal composition of the present invention is mainly used in the disease of crops such as fruit tree, flowers, cotton, wheat, paddy rice, rape, vegetables, especially oomycetes disease control.
Compared with prior art, the beneficial effect of the present invention's generation is: (1) is compared with single agent, and this bactericidal composition has tangible synergy to disease, has improved control efficiency; (2) can significantly reduce the field dosage, effectively reduce environmental pollution and residue of pesticide, reduce and produce and use cost; (3) mechanism of action of active ingredient is different in the bactericidal composition, helps the generation speed of avoiding disease to produce resistance and delaying disease drug resistance.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention is limited to these examples absolutely not.
The combination of active principles of different agricultural chemicals being carried out composite, is a kind of mode effectively and efficiently that solves some problems in the agricultural chemicals list agent application process at present.The agricultural chemicals of different cultivars shows three kinds of type of action after mixing usually, and promptly summation action, synergistic effect and antagonism act on but which kind of is specially, and be unpredictable, has only by a large amount of tests and just can know.Compound synergic is well filled a prescription, and can improve actual control efficiency, reduces the usage amount of agricultural chemicals, also helps the generation of delaying drug resistance, is the important means of integrated control.
The present composition is a kind of active ingredient with cyanogen frost azoles (A), with a kind of (B) in metalaxyl, Metalaxyl-M, M 9834, the spirit of furalaxyl, Evil frost, white urea cyanogen or the Propamocarb is another kind of active ingredient, combination has obvious role in synergism to the oomycetes disease between them, and be not only the simple addition of two kinds of medicament effects, specifically be illustrated with following biologicall test example.
Biologicall test example 1: cyanogen frost azoles and metalaxyl, Evil frost spirit or the composite toxicity test of white urea cyanogen to cucumber downy mildew
Subjects: cucumber downy mildew germ
Pot-culture method is adopted in this test.Dip in the sporangium that the distilled water of getting about 10 ℃ washes the cucumber leaves back side that has Pseudoperonospora cubensis of picking up from the field with writing brush, be made into 3 * 10
5The suspension of individual sporangium/mL.Choose two leaf period cucumber seedlings of growing way unanimity, each processing selects for use 5 basins for examination melon seedling, sprays the about 5mL of every processing under 50PSI pressure with the Potter spray tower.Each medicament is provided with 5 concentration gradients, is blank with what spray the equivalent clear water.24h spray inoculation bacterium of downy mildew of cucumber sporangia suspension after the chemicals treatment, after the inoculation cucumber seedling placed climatic cabinate (relative moisture 100%, temperature 15-20 ℃) cultivate, keep 15-24 ℃ of temperature, relative moisture about 90% to preserve moisture and bring out behind the 24h, 5d " Invest, Then Investigate " disease index, and calculate control efficiency.
Grade scale
0 grade: blade does not have scab;
1 grade: lesion area accounts for below 5% of whole blade area;
3 grades: lesion area accounts for the 6-10% of whole blade area;
5 grades: lesion area accounts for the 11-25% of whole blade area;
7 grades: lesion area accounts for the 26-50% of whole blade area;
9 grades: lesion area accounts for more than 50% of whole blade area.
The drug effect computational methods:
Control efficiency is converted into probability value (y), and liquor strength (μ g/ml) converts logarithm value (x) to, calculates concentration EC in virulence equation and the inhibition with method of least squares
50, according to the toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (the ATI)/theoretical toxicity index (TTI) of mixture] * 100
When CTC≤80, then the composition performance is for antagonism, and when 80＜CTC＜120, then the composition performance is for summation action, and when CTC 〉=120, then the composition performance is synergistic effect.
Toxicity test result referring to table 1 to table 3
The serial proportioning of table 1 cyanogen frost azoles and metalaxyl is to the Toxicity Determination result of cucumber downy mildew
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.27 | ??100 | ??/ | ??/ |
| Metalaxyl | ??45.67 | ??0.6 | ??/ | ??/ |
| Cyanogen frost azoles 50: metalaxyl 1 | ??0.21 | ??128.6 | ??98.1 | ??131.1 |
| Cyanogen frost azoles 20: metalaxyl 1 | ??0.19 | ??142.1 | ??95.3 | ??149.2 |
| Cyanogen frost azoles 10: metalaxyl 1 | ??0.17 | ??158.8 | ??91.0 | ??174.6 |
| Cyanogen frost azoles 5: metalaxyl 1 | ??0.15 | ??180.0 | ??83.4 | ??215.7 |
| Cyanogen frost azoles 1: metalaxyl 1 | ??0.21 | ??128.6 | ??50.3 | ??255.6 |
| Cyanogen frost azoles 1: metalaxyl 5 | ??0.58 | ??46.6 | ??17.2 | ??271.3 |
| Cyanogen frost azoles 1: metalaxyl 10 | ??1.19 | ??22.7 | ??9.6 | ??235.6 |
| Cyanogen frost azoles 1: metalaxyl 20 | ??2.98 | ??9.1 | ??5.3 | ??170.1 |
| Cyanogen frost azoles 1: metalaxyl 50 | ??6.95 | ??3.9 | ??2.5 | ??152.9 |
Result of the test shows, cyanogen frost azoles and the composite control cucumber downy mildew of metalaxyl, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 131.1, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 170.1, and synergistic effect is more obvious.
Table 2 cyanogen frost Zuo Yu the serial proportioning of Evil frost spirit to the Toxicity Determination result of cucumber downy mildew
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.27 | ??100 | ??/ | ??/ |
| The spirit of Evil frost | ??48.37 | ??0.6 | ??/ | ??/ |
| Cyanogen frost azoles 50 ∶ Evil frost spirit 1 | ??0.19 | ??142.1 | ??98.1 | ??144.9 |
| Cyanogen frost azoles 20 ∶ Evil frost spirit 1 | ??0.17 | ??158.8 | ??95.3 | ??166.7 |
| Cyanogen frost azoles 10 ∶ Evil frost spirit 1 | ??0.15 | ??180.0 | ??91.0 | ??197.9 |
| Cyanogen frost azoles 5 ∶ Evil frost spirit 1 | ??0.14 | ??192.9 | ??83.4 | ??231.2 |
| Cyanogen frost azoles 1 ∶ Evil frost spirit 1 | ??0.21 | ??128.6 | ??50.3 | ??255.7 |
| Cyanogen frost azoles 1 ∶ Evil frost spirit 5 | ??0.53 | ??50.9 | ??17.1 | ??297.4 |
| Cyanogen frost azoles 1 ∶ Evil frost spirit 10 | ??1.18 | ??22.9 | ??9.6 | ??238.4 |
| Cyanogen frost azoles 1 ∶ Evil frost spirit 20 | ??2.71 | ??10.0 | ??5.3 | ??188.2 |
| Cyanogen frost azoles 1 ∶ Evil frost spirit 50 | ??7.03 | ??3.8 | ??2.5 | ??153.1 |
Result of the test shows, cyanogen frost Zuo is Yu the clever composite control cucumber downy mildew of Evil frost, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 144.9, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 197.9, and synergistic effect is more obvious.
The serial proportioning of table 3 cyanogen frost azoles and white urea cyanogen is to the Toxicity Determination result of cucumber downy mildew
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.27 | ??100 | ??/ | ??/ |
| Frost urea cyanogen | ??12.67 | ??2.1 | ??/ | ??/ |
| Cyanogen frost azoles 50: white urea cyanogen 1 | ??0.19 | ??142.1 | ??98.1 | ??144.9 |
| Cyanogen frost azoles 20: white urea cyanogen 1 | ??0.17 | ??158.8 | ??95.3 | ??166.6 |
| Cyanogen frost azoles 10: white urea cyanogen 1 | ??0.16 | ??168.8 | ??91.1 | ??185.2 |
| Cyanogen frost azoles 5: white urea cyanogen 1 | ??0.15 | ??180.0 | ??83.7 | ??215.1 |
| Cyanogen frost azoles 1: white urea cyanogen 1 | ??0.21 | ??128.6 | ??51.1 | ??251.8 |
| Cyanogen frost azoles 1: white urea cyanogen 5 | ??0.48 | ??56.3 | ??18.4 | ??305.0 |
| Cyanogen frost azoles 1: white urea cyanogen 10 | ??1.11 | ??24.3 | ??11.0 | ??220.6 |
| Cyanogen frost azoles 1: white urea cyanogen 20 | ??2.22 | ??12.2 | ??6.8 | ??179.1 |
| Cyanogen frost azoles 1: white urea cyanogen 50 | ??4.35 | ??6.2 | ??4.1 | ??153.3 |
Result of the test shows, cyanogen frost azoles and the composite control cucumber downy mildew of white urea cyanogen, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 144.9, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 179.1, and synergistic effect is more obvious.
Biologicall test example 2: cyanogen frost azoles and the composite toxicity test of Metalaxyl-M, M 9834, furalaxyl or Propamocarb to tomato late blight
Subjects: tomato late blight germ
Pot-culture method is adopted in test.Tomato is potted plant to be cultured to 2-4 leaf periods, numbers standby.To test with pathogen and on the suitable culture base, cultivate, after waiting to produce sporangium, sporangium be washed, filter, make 1 * 10 with double gauze with sterile water
5Individual sporangium/mL sporangia suspension.According to the medicament activity,, 5-7 series mass concentration is set according to the medicament activity.Soup is evenly sprayed in the blade face to all wetting, treat that the soup natural air drying is standby.Every processing 3 basins repeat for 4 times, and establish the processing that only contains solvent and surfactant and do not contain effective composition and make blank.Behind the chemicals treatment 24h, on tomato leaf, evenly spray inoculation with the crop sprayer.After the inoculation,, cultivated 7 days under the condition of relative moisture more than 90% at temperature 18-20 ℃.Add up the lesion area on every strain tomato leaf, and calculate disease index and control efficiency.30 leaves are investigated in every processing at least, and stage division is:
0 grade; Anosis;
1 grade: a small amount of little scab is only arranged on the blade, and scab accounts for leaf area below 10%;
3 grades: scab accounts for leaf area 10%-25% on the blade;
5 grades: scab accounts for leaf area 26%-50% on the blade;
7 grades: scab accounts for leaf area more than 50% on the blade;
9 grades: full leaf morbidity is withered.
Drug effect calculating, toxicity index and co-toxicity coefficient calculate, analytical method is the same.
Toxicity test the results are shown in Table 4 to table 7.
The serial proportioning of table 4 cyanogen frost azoles and Propamocarb is to the Toxicity Determination result of tomato late blight
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.32 | ??100 | ??/ | ??/ |
| Propamocarb | ??52.34 | ??0.6 | ??/ | ??/ |
| Cyanogen frost azoles 50: Propamocarb 1 | ??0.23 | ??139.1 | ??98.1 | ??141.9 |
| Cyanogen frost azoles 20: Propamocarb 1 | ??0.21 | ??152.4 | ??95.3 | ??160.0 |
| Cyanogen frost azoles 10: Propamocarb 1 | ??0.18 | ??177.8 | ??91.0 | ??195.4 |
| Cyanogen frost azoles 5: Propamocarb 1 | ??0.17 | ??188.2 | ??83.4 | ??225.6 |
| Cyanogen frost azoles 1: Propamocarb 1 | ??0.24 | ??133.3 | ??50.3 | ??265.0 |
| Cyanogen frost azoles 1: Propamocarb 5 | ??0.65 | ??49.2 | ??17.2 | ??286.6 |
| Cyanogen frost azoles 1: Propamocarb 10 | ??1.43 | ??22.4 | ??9.6 | ??232.0 |
| Cyanogen frost azoles 1: downy mildew becomes 20 | ??3.16 | ??10.1 | ??5.3 | ??189.5 |
| Cyanogen frost azoles 1: downy mildew becomes 50 | ??8.15 | ??3.9 | ??2.6 | ??153.4 |
Result of the test shows, cyanogen frost azoles and the composite control tomato late blight of Propamocarb, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 141.9, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 189.5, and synergistic effect is more obvious.
The serial proportioning of table 5 cyanogen frost azoles and Metalaxyl-M is to the Toxicity Determination result of tomato late blight
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.42 | ??100 | ??/ | ??/ |
| Metalaxyl-M | ??42.34 | ??1.0 | ??/ | ??/ |
| Cyanogen frost azoles 50: Metalaxyl-M 1 | ??0.28 | ??150.0 | ??98.1 | ??153.0 |
| Cyanogen frost azoles 20: Metalaxyl-M 1 | ??0.25 | ??168.0 | ??95.3 | ??176.3 |
| Cyanogen frost azoles 10: Metalaxyl-M 1 | ??0.22 | ??190.9 | ??91.0 | ??209.8 |
| Cyanogen frost azoles 5: Metalaxyl-M 1 | ??0.21 | ??200.0 | ??83.5 | ??239.5 |
| Cyanogen frost azoles 1: Metalaxyl-M 1 | ??0.33 | ??127.3 | ??50.5 | ??252.0 |
| Cyanogen frost azoles 1: Metalaxyl-M 5 | ??0.82 | ??51.2 | ??17.5 | ??292.8 |
| Cyanogen frost azoles 1: Metalaxyl-M 10 | ??1.82 | ??23.1 | ??10.0 | ??230.9 |
| Cyanogen frost azoles 1: Metalaxyl-M 20 | ??3.56 | ??11.8 | ??5.7 | ??206.7 |
| Cyanogen frost azoles 1: Metalaxyl-M 50 | ??8.75 | ??4.8 | ??2.9 | ??163.6 |
Result of the test shows, cyanogen frost azoles and the clever composite control tomato late blight of smart Shen frost, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 153.0, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 206.7, and synergistic effect is more obvious.
The serial proportioning of table 6 cyanogen frost azoles and M 9834 is to the Toxicity Determination result of tomato late blight
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.42 | ??100 | ??/ | ??/ |
| M 9834 | ??43.34 | ??1.0 | ??/ | ??/ |
| Cyanogen frost azoles 50: M 9834 1 | ??0.32 | ??131.3 | ??98.1 | ??133.8 |
| Cyanogen frost azoles 20: M 9834 1 | ??0.3 | ??140.0 | ??95.3 | ??146.9 |
| Cyanogen frost azoles 10: M 9834 1 | ??0.24 | ??175.0 | ??91.0 | ??192.3 |
| Cyanogen frost azoles 5: M 9834 1 | ??0.23 | ??182.6 | ??83.5 | ??218.7 |
| Cyanogen frost azoles 1: M 9834 1 | ??0.33 | ??127.3 | ??50.5 | ??252.1 |
| Cyanogen frost azoles 1: M 9834 5 | ??0.78 | ??53.8 | ??17.5 | ??308.1 |
| Cyanogen frost azoles 1: M 9834 10 | ??1.47 | ??28.6 | ??10.0 | ??286.5 |
| Cyanogen frost azoles 1: M 9834 20 | ??3.61 | ??11.6 | ??5.7 | ??204.7 |
| Cyanogen frost azoles 1: M 9834 50 | ??9.63 | ??4.4 | ??2.9 | ??149.8 |
Result of the test shows, cyanogen frost azoles and the composite control tomato late blight of M 9834, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 133.8, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 192.3, and synergistic effect is more obvious.
The serial proportioning of table 7 cyanogen frost azoles and furalaxyl is to the Toxicity Determination result of tomato late blight
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Cyanogen frost azoles | ??0.42 | ??100 | ??/ | ??/ |
| Furalaxyl | ??39.34 | ??1.1 | ??/ | ??/ |
| Cyanogen frost azoles 50: furalaxyl 1 | ??0.31 | ??135.5 | ??98.1 | ??138.2 |
| Cyanogen frost azoles 20: furalaxyl 1 | ??0.28 | ??150.0 | ??95.3 | ??157.4 |
| Cyanogen frost azoles 10: furalaxyl 1 | ??0.22 | ??190.9 | ??91.0 | ??209.8 |
| Cyanogen frost azoles 5: furalaxyl 1 | ??0.23 | ??182.6 | ??83.5 | ??218.7 |
| Cyanogen frost azoles 1: furalaxyl 1 | ??0.35 | ??120.0 | ??50.5 | ??237.5 |
| Cyanogen frost azoles 1: furalaxyl 5 | ??0.82 | ??51.2 | ??17.6 | ??291.7 |
| Cyanogen frost azoles 1: furalaxyl 10 | ??1.67 | ??25.1 | ??10.1 | ??250.0 |
| Cyanogen frost azoles 1: furalaxyl 20 | ??3.62 | ??11.6 | ??5.8 | ??200.8 |
| Cyanogen frost azoles 1: furalaxyl 50 | ??9.33 | ??4.5 | ??3.0 | ??149.7 |
Result of the test shows, cyanogen frost azoles and the composite control tomato late blight of furalaxyl, and proportioning is 50: 1-1: in the time of between 50, co-toxicity coefficient all more than 138.2, has synergistic effect, and 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 200.8, and synergistic effect is more obvious.
Above measurement result shows, the proportioning of a kind of (B) composite control cucumber downy mildew and tomato late blight is 50 in cyanogen frost azoles (A) and metalaxyl, Metalaxyl-M, M 9834, the spirit of furalaxyl, Evil frost, white urea cyanogen or the Propamocarb: 1-1: in the time of between 50, has synergistic effect, wherein 10: 1-1: in the time of between 20, co-toxicity coefficient is more than 170.1, and synergistic effect is more obvious.
Bactericidal composition of the present invention can be prepared into known method and be fit to wetting powder, suspending agent, water dispersible granules, missible oil, microemulsion or the aqueous emulsion that agricultural is used.Below describe with specific embodiment, percentage is mass percent in the prescription.(B) is a kind of in active component present specification middle finger cyanogen frost azoles (A) and metalaxyl, Metalaxyl-M, M 9834, the spirit of furalaxyl, Evil frost, white urea cyanogen or the Propamocarb, below repeats no more.
Embodiment 1:20.4% cyanogen frost azoles metalaxyl wetting powder
Cyanogen frost azoles 20%
Metalaxyl 0.4%
Lauryl sodium sulfate (wetting agent) 2%
Sodium lignin sulfonate (dispersant) 5%
Naphthalene sulfonate (dispersant) 3%
Kaolin (filler) complements to 100%.
Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, make 20.4% cyanogen frost azoles metalaxyl wetting powder, be used for the cucumber downy mildew field trial.
Test method: (the white azoles valid density of cyanogen is 50 μ g/ml by 4000 times with 20.4% cyanogen frost azoles metalaxyl wetting powder, metalaxyl valid density is 1 μ g/ml) the thin up spraying, two contrast medicaments are used with quadrat method and are used, diluted concentration is respectively 2000 times of 15% cyanogen frost azole suspending agents (cyanogen frost azoles valid density is 75 μ g/ml) and 500 times of 30% metalaxyl water dispersible granules (valid density is 600 μ g/ml), 7 days and 15 days investigation prevention effects behind the medicine.
Result of the test: the preventive effect of 7 days and 15 days is respectively 87.2%, 86.2% behind the 20.4% cyanogen frost azoles metalaxyl wetting powder medicine, and the preventive effect of 7 days and 15 days is respectively 82.1%, 80.3% and 72.3%, 71.7% behind white azole suspending agent of contrast medicament 15% cyanogen and the 30% metalaxyl water dispersible granules medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of metalaxyl, to the preventive effect of cucumber downy mildew significantly better than single agent.
The clever suspending agent of embodiment 2:5% cyanogen Shuan Zuo Evil frost
Cyanogen frost azoles 2.5%
Evil frost spirit 2.5%
Methyl naphthalene sulfonate formaldehyde condensate (wetting dispersing agent) 10%
Xanthans (thickener) 1%
Bentonite (carrier) 1%
Glycerine (antifreeze) 1%
Water complements to 100%.
The ratio of each component such as active component, wetting dispersing agent, thickener, carrier and water in prescription mixed, after grinding and/or high speed shear, obtain the clever suspending agent of 5% cyanogen Shuan Zuo Evil frost.Be used for the peronophythora litchi field trial.
Test method: the clever suspending agent of 5% cyanogen Shuan Zuo Evil frost is sprayed by 500 times of (cyanogen frost azoles valid density is that the clever valid density of 50 μ g/ml ， Evil frost is 50 μ g/ml) thin ups.Two contrast medicaments are used with quadrat method and are used, and diluted concentration is respectively 15% cyanogen frost azole suspending agent 2000 times of (valid density is 75 μ g/ml) and 40% Evil frost, 500 times of clever wetting powders (valid density is 800 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the preventive effect of 7 days and 15 days is respectively 89.6%, 88.2% behind the clever suspending agent medicine of 10% cyanogen Shuan Zuo Evil frost.The preventive effect of 7 days and 15 days is respectively 84.9%, 83.2% and 81.4%, 80.6% behind contrast medicament 15% cyanogen frost azole suspending agent and the clever wetting powder medicine of 40% Evil frost.Cyanogen frost Zuo is Yu the composite back synergistic effect of Evil frost spirit is obvious, to the preventive effect of peronophythora litchi significantly better than single agent.
Embodiment 3:20.4% cyanogen frost azoles furalaxyl wetting powder
Cyanogen frost azoles 0.4%
Furalaxyl 20%
Lauryl sodium sulfate (wetting agent) 2%
Sodium lignin sulfonate (dispersant) 5%
Naphthalene sulfonate (dispersant) 3%
Kaolin (filler) complements to 100%.
Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, make 20.4% cyanogen frost azoles furalaxyl wetting powder.Be used for the downy mildew of lettuce field control effectiveness test.
Test method: choose the consistent lettuce vegetable plot of downy mildew of lettuce morbidity, (the white azoles valid density of cyanogen is 4 μ g/ml by 100 times with 20.4% cyanogen frost azoles furalaxyl wetting powder, furalaxyl valid density is 200 μ g/ml) the thin up spraying, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 4% cyanogen frost azole suspending agent 1000 times of (valid density is 40 μ g/ml) and 500 times of 20% furalaxyl wetting powders (valid density is 400 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the control efficiency of 7 days and 15 days is respectively 85.1%, 84.7% behind the 20.4% cyanogen frost azoles furalaxyl wetting powder medicine.The preventive effect of 7 days and 15 days is respectively 74.5%, 73.3% and 79.3%, 78.5% behind contrast medicament 4% cyanogen frost azole suspending agent and the 20% furalaxyl wetting powder medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of furalaxyl, to the preventive effect of downy mildew of lettuce significantly better than single agent.
Embodiment 4:80% cyanogen frost azoles frost urea cyanogen water dispersible granules
Cyanogen frost azoles 30%
Frost urea cyanogen 50%
Negel (dispersant) 0.5%
Sodium lignin sulfonate (dispersant) 0.5%
Lauryl sodium sulfate (wetting agent) 0.5%
Kaolin (filler) is to 100%
Active component, dispersant, wetting agent and the filler ratio in prescription is mixed, become wetting powder, add a certain amount of water mixing extruding again and make material through comminution by gas stream.Obtain 80% cyanogen frost azoles frost urea cyanogen water dispersible granules after the drying screening.Be used for the tomato late blight field trial.
Test method: 5000 times (cyanogen frost azoles valid density is 60 μ g/ml with 80% cyanogen frost azoles frost urea cyanogen water dispersible granules, frost urea cyanogen valid density is 100 μ g/ml) the thin up spraying, the contrast medicament, with using with quadrat method, diluted concentration is respectively 20% cyanogen frost, 2000 times of water dispersible granules of azoles (valid density is 100 μ g/ml), 1000 times of 20% white urea cyanogen water dispersible granules (valid density is 200 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the control efficiency of 7 days and 15 days is respectively 98.9%, 97.7% behind the 80% cyanogen frost azoles frost urea cyanogen water dispersible granules medicine.The preventive effect of 7 days and 15 days is respectively 86.2%, 85.3% and 85.6%, 74.4% behind the contrast medicament medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of white urea cyanogen, to the preventive effect of tomato late blight significantly better than single agent.
Embodiment 5:30% cyanogen frost azoles Metalaxyl-M wetting powder
Cyanogen frost azoles 10%
Metalaxyl-M 20%
Lauryl sodium sulfate (wetting agent) 2%
Sodium lignin sulfonate (dispersant) 5%
Naphthalene sulfonate (dispersant) 3%
Kaolin (filler) complements to 100%.
Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, make 30% cyanogen frost azoles Metalaxyl-M wetting powder.Be used for balsam pear cream mildew field trial.
Test method: (the white azoles valid density of cyanogen is 50 μ g/ml by 2000 times with 30% cyanogen frost azoles Metalaxyl-M wetting powder, Metalaxyl-M valid density is 100 μ g/ml) the thin up spraying, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 8% cyanogen frost azole suspending agent 1000 times of (valid density is 80 μ g/ml) and 500 times of 25% Metalaxyl-M water dispersible granules (valid density is 500 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: 30% cyanogen frost azoles Metalaxyl-M wetting powder, the control efficiency of 7 days and 15 days is respectively 94.6%, 93.4% behind the medicine.The preventive effect of 7 days and 15 days is respectively 85.6%, 84.3% and 84.3%, 83.5% behind contrast medicament 8% cyanogen frost azole suspending agent and the 25% Metalaxyl-M water dispersible granules medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of Metalaxyl-M, to the preventive effect of balsam pear cream mildew significantly better than single agent.
Embodiment 6:30% cyanogen frost azoles Propamocarb wetting powder
Cyanogen frost azoles 5%
Propamocarb 25%
Lauryl sodium sulfate (wetting agent) 2%
Sodium lignin sulfonate (dispersant) 5%
Naphthalene sulfonate (dispersant) 3%
Kaolin (filler) complements to 100%.
Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, make 30% cyanogen frost azoles Propamocarb wetting powder.Be used for the downy mildew of garpe field trial.
Test method: (the white azoles valid density of cyanogen is 50 μ g/ml by 1000 times with 30% cyanogen frost azoles Propamocarb wetting powder, Propamocarb valid density is 250 μ g/ml) the thin up spraying, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 15% cyanogen frost azole suspending agent 2000 times of (valid density is 75 μ g/ml) and 1000 times in 60% Propamocarb aquas (valid density is 600 μ g/ml).7 days and 15 days investigation prevention effects behind the medicine.
Result of the test: the preventive effect of 7 days and 15 days is respectively 97.6%, 96.4% behind the 30% cyanogen frost azoles Propamocarb wetting powder medicine, and the preventive effect of 7 days and 15 days is respectively 84.3%, 83.4% and 83.4%, 82.6% behind the white azole suspending agent of contrast medicament 15% cyanogen, the 60% downy mildew salt water agent medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of Propamocarb, to the preventive effect of downy mildew of garpe significantly better than single agent.
Embodiment 7:30% cyanogen frost azoles M 9834 missible oil
Cyanogen frost azoles 10%
M 9834 20%
N-Methyl pyrrolidone (solvent) 10%
DMF (solvent) 10%
The newborn 1601# of farming (emulsifier) 5%
The newborn 500# of farming (emulsifier) 5%
Dimethylbenzene (solvent) complements to 100%.
Active component, solvent, emulsifier are added in the mixing kettle successively in the ratio of prescription, stir, make 30% cyanogen frost azoles M 9834 missible oil.Be used for the capsicum epidemic disease field trial.
Test method: (the white azoles valid density of cyanogen is 50 μ g/ml by 2000 times with 30% cyanogen frost azoles M 9834 missible oil, M 9834 valid density is 100 μ g/ml) the thin up spraying, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 8% cyanogen frost azole suspending agent 1000 times of (valid density is 80 μ g/ml) and 1000 times of 60% M 9834 water dispersible granules (valid density is 600 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the control efficiency of 7 days and 15 days is respectively 91.1%, 90.3% behind the 30% cyanogen frost azoles M 9834 missible oil medicine.The preventive effect of 7 days and 15 days is respectively 84.6%, 83.5% and 82.5%, 80.2% behind contrast medicament 8% cyanogen frost azole suspending agent and the 60% M 9834 water dispersible granules medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of M 9834, to the preventive effect of capsicum epidemic disease significantly better than single agent.
The clever microemulsion of embodiment 8:15% cyanogen Shuan Zuo Evil frost
Cyanogen frost azoles 5%
Evil frost spirit 10%
N-Methyl pyrrolidone (solvent) 10%
Isopropyl alcohol (cosolvent) 10%
The newborn 500# of farming (emulsifier) 5%
The newborn 1601# of farming (emulsifier) 5%
Water complements to 100%.
Former medicine, solvent, cosolvent, emulsifier are added together, make and be dissolved into even oil phase; With water-soluble component and the mixed water that gets of water; Under high-speed stirred, oil phase is mixed with water, make the clever microemulsion of 15% cyanogen Shuan Zuo Evil frost.Be used for the potato late blight field trial.
Test method: the clever microemulsion of 15% cyanogen Shuan Zuo Evil frost is sprayed by 1000 times of (cyanogen frost azoles valid density is that the clever valid density of 50 μ g/ml Evil frost is 100 μ g/ml) thin ups, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 8% cyanogen frost azole suspending agent 1000 times of (valid density is 80 μ g/ml) and 40% Evil frost, 500 times of dispersible granules of Lingshui Spring (valid density is 800 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the control efficiency of 7 days and 15 days is respectively 91.1%, 89.3% behind the clever microemulsion medicine of 15% cyanogen Shuan Zuo Evil frost.The preventive effect of 7 days and 15 days is respectively 81.6%, 80.5% and 84.5%, 83.2% behind the contrast medicament medicine.Cyanogen frost Zuo is Yu the composite back synergistic effect of Evil frost spirit is obvious, to the preventive effect of potato late blight significantly better than single agent.
Embodiment 9:20% cyanogen frost azoles frost urea cyanogen aqueous emulsion
Cyanogen frost azoles 5%
Frost urea cyanogen 15%
N-Methyl pyrrolidone (solvent) 10%
Calcium dodecyl benzene sulfonate (emulsifier) 5%
The newborn 600# of farming (emulsifier) 5%
Water complements to 100%
Active substance, solvent, emulsifier are added together, make and be dissolved into even oil phase; Oil phase is added in the entry, under high-speed stirred, oil phase is mixed with water, make 20% cyanogen frost azoles frost urea cyanogen aqueous emulsion.Be used for cucumber eqpidemic disease field trial.
Test method: (the white azoles valid density of cyanogen is 50 μ g/ml by 2000 times with 20% cyanogen frost azoles frost urea cyanogen aqueous emulsion, frost urea cyanogen valid density is 150 μ g/ml) the thin up spraying, the contrast medicament is used with quadrat method and is used, and diluted concentration is respectively 8% cyanogen frost azole suspending agent 1000 times of (valid density is 80 μ g/ml) and 1000 times of 30% white urea cyanogen water dispersible granules (valid density is 300 μ g/ml).7 days and 15 days difference investigation prevention effects behind the medicine.
Result of the test: the control efficiency of 7 days and 15 days is respectively 97.7%, 96.3% behind the 20% cyanogen frost azoles frost urea cyanogen aqueous emulsion medicine.The preventive effect of 7 days and 15 days is respectively 82.6%, 81.2% and 87.2%, 78.2% behind the contrast medicament medicine.Cyanogen frost azoles is obvious with the composite synergistic effect afterwards of white urea cyanogen, to the preventive effect of cucumber eqpidemic disease significantly better than single agent.
Claims (7)
1, a kind of bactericidal composition, it is characterized in that: in containing active ingredient cyanogen frost azoles (A) in the described bactericidal composition and metalaxyl, the spirit of Metalaxyl-M, Evil frost, M 9834, furalaxyl, white urea cyanogen or downy mildew becoming a kind of (B), the mass ratio of A and B is 50: 1-1: 50.
2, according to claim 1, it is characterized in that: the mass ratio of active ingredient is 10: 1-1: 20.
3, according to claim 1 or 2, it is characterized in that: the gross mass percentage composition of active ingredient in bactericidal composition is 5%-80%.
4, according to claim 3, it is characterized in that: the formulation of described bactericidal composition is missible oil, aqueous emulsion, microemulsion, wetting powder, suspending agent or water dispersible granules.
5, according to claim 1, it is characterized in that: described bactericidal composition is used to prevent and treat crop pest.
6, the application of bactericidal composition according to claim 5 is characterized in that: described crop is flowers, paddy rice, wheat, fruit tree, cotton, rape, vegetables.
7, according to claim 5 or 6, it is characterized in that: described bactericidal composition is used to prevent and treat the Oomycete disease.
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| CN102763644A (en) * | 2012-06-30 | 2012-11-07 | 广东中迅农科股份有限公司 | Cyazofamid microemulsion and preparation method thereof |
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| CN105432697A (en) * | 2015-12-27 | 2016-03-30 | 胡凡营 | Biological pesticide containing cyazofamid and cymoxanil and preparation method of biological pesticide |
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| CN101874488B (en) * | 2010-03-13 | 2012-11-07 | 陕西美邦农资贸易有限公司 | Bactericide composition containing imazalil and cyazofamid |
| CN105724386A (en) * | 2012-05-25 | 2016-07-06 | 陕西韦尔奇作物保护有限公司 | Fluxapyroxad-containing bacteriocidal composition |
| CN105724386B (en) * | 2012-05-25 | 2018-06-26 | 陕西韦尔奇作物保护有限公司 | A kind of bactericidal composition of Fluxapyroxad-containsterilization |
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| CN102763644A (en) * | 2012-06-30 | 2012-11-07 | 广东中迅农科股份有限公司 | Cyazofamid microemulsion and preparation method thereof |
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| CN103563948B (en) * | 2013-11-19 | 2015-07-22 | 西安北农华农作物保护有限公司 | Bactericidal composition containing cyazofamid |
| CN103858911A (en) * | 2014-03-14 | 2014-06-18 | 曹荣成 | Compounded fungicidal composition containing cyazofamid and cymoxanil |
| CN104920369A (en) * | 2015-06-08 | 2015-09-23 | 柳州市天姿园艺有限公司 | Composition for controlling phytophthora root rot of vaniot houtt |
| CN105028417A (en) * | 2015-06-08 | 2015-11-11 | 柳州市天姿园艺有限公司 | Composition for preventing and treating phytophthora root rot of cymbidium kanran |
| CN105432697A (en) * | 2015-12-27 | 2016-03-30 | 胡凡营 | Biological pesticide containing cyazofamid and cymoxanil and preparation method of biological pesticide |
| CN105941408A (en) * | 2016-06-13 | 2016-09-21 | 浙江禾本科技有限公司 | Pesticide water suspending agent containing cyazofamid and metalaxyl-M composition and preparation method of pesticide water suspending agent |
| CN115299452A (en) * | 2022-06-30 | 2022-11-08 | 陕西美邦药业集团股份有限公司 | Bactericidal composition containing oxadixyl |
| CN115299452B (en) * | 2022-06-30 | 2024-02-27 | 陕西美邦药业集团股份有限公司 | Bactericidal composition containing oxadixyl |
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