CN101642129A - Sterilization composite - Google Patents

Abstract

The invention discloses a sterilization composite. The active components thereof is cyprodinil (A) and triazole fungicide (B), B is difenoconazole, hexaconazole, propiconazole, flusilazole, penconazole, triazolone, diniconazole, triflumizole or flutriafol; the mass ratio of (A) and (B) is 50-1:1-50. The active components (A) and (B) have obvious synergized effect after compounding in curing and preventing diseases, especially fungal diseases, of crops of fruit tree, cotton, rice, vegetable and the like.

Description

Bactericidal composition

technical field

The invention relates to a bactericidal composition, in particular to a bactericidal composition containing cyprodinil.

Background technique

In recent years, due to single and unscientific drug use, many diseases have become resistant to the currently used pesticides, such as rice blast, gray mold, powdery mildew, scab, and surplus blight, which have become one of the main methods of chemical control. big problem. At the same time, frequent application of pesticides has caused increased burden on farmers and increased environmental pollution. Therefore, there is an urgent need for new varieties of fungicides with high efficiency, low toxicity and environmental protection.

Cyprodinil, English common name Cyprodinil, chemical name 4-cyclopropyl-6-methyl-N-phenylpyrimidin-2-amine, is a protective fungicide, the mechanism of action is methionine biosynthesis inhibitor, Acts after spore germination, inhibits fungal penetration process and mycelium growth. No cross-resistance with fungicides such as triazoles, imidazoles, morpholines, and phenylpyrroles. Mainly used for the control of Botrytis cinerea, powdery mildew, scab, glume blight and wheat eye streak disease. However, the relative price of using alone is relatively high, and the types of diseases that can be prevented and controlled are not enough.

The bactericidal mechanism of triazole fungicides is relatively complex, mainly inhibiting the biosynthesis of ergosterol in the bacteria, thereby inhibiting or interfering with the development of spores and haustoria, the growth of mycelia and the formation of spores. It has a good effect on powdery mildew, but the powdery mildew bacteria in many areas are resistant to triazoles, and the effect of single use is not ideal. In addition, most triazole fungicides are likely to cause phytotoxicity to crops during use.

In view of this, it is indeed necessary to provide a safe and efficient fungicidal composition suitable for agricultural use.

Contents of the invention

The object of the present invention is to provide a safe and efficient bactericidal composition suitable for agricultural use and having excellent control effect on diseases.

In order to solve the above technical problems, the inventor, through a large number of bioassays and screenings, unexpectedly discovered that the combination of cyprodinil and triazole fungicides in a certain proportion has a significant synergistic effect on rice blast, powdery mildew, gray mold and other diseases effect.

On the basis of the above findings, through the quantitative analysis of the combined effect of the composition, the technical solution of the present invention has been formed, that is, using cyprodinil (A) as an active ingredient and a triazole fungicide ( B) is another active ingredient, and the mass ratio of (A) to (B) is 50:1-1:50; the triazole fungicides are difenoconazole, hexaconazole, propiconazole, fluorine Silazole, penconazole, triadimefon, diniconazole, fluconazole, or triconazole.

As an improvement of the present invention, the preferred ratio of active ingredients (A) to (B) is 20:1-1:20.

The composition of the present invention can be prepared into a dosage form suitable for agricultural use by known methods, and the total content of active ingredients in the preparation is 5%-80%.

The adjuvants used in the composition of the present invention include dispersants, wetting agents, antifreeze agents, penetrating agents, etc. and other known substances that are beneficial to the stability of active ingredients in storage and use and the exertion of drug effects, all of which are in pesticide formulations. There are no special restrictions on the commonly used or allowed ingredients, and the specific ingredients and dosage are determined through simple tests according to the formula requirements.

The composition described in the present invention can be provided in the form of a finished preparation, that is, the substances in the composition have been mixed, and the ingredients of the composition can also be provided in a single dose form, directly mixed in a barrel or tank before use, and then diluted to the required dosage. concentration.

The fungicidal composition of the invention is mainly used for the prevention and treatment of diseases of fruit trees, cotton, rice, vegetables and other crops, especially fungal diseases.

Compared with the prior art, the beneficial effects produced by the present invention are: (1) Compared with a single agent, the fungicidal composition has obvious synergistic effect on diseases such as rice blast, powdery mildew, gray mold, etc., and improves the control effect ; Moreover, because the amount of triazole fungicides is reduced, the safety of crops is improved. (2) It can greatly reduce the dosage of pesticides in the field, effectively reduce environmental pollution and pesticide residues, and reduce production and use costs; (3) The mechanisms of action of the active ingredients in the fungicidal composition are different, which is conducive to overcoming and delaying the generation of disease resistance .

Detailed ways

In order to make the object, technical solution and advantages of the present invention more clear, the present invention is illustrated with the following specific examples, but the present invention is by no means limited to these examples.

Compounding the active ingredients of different pesticides is an effective and quick way to solve some problems in the process of single-dose application of pesticides. When different varieties of pesticides are mixed, they usually show three types of effects, namely additive effect, synergistic effect and antagonistic effect, but the specific effect cannot be predicted, and can only be known through a large number of experiments. The formula with good compound synergy can improve the actual control effect, reduce the use of pesticides, and help delay the emergence of resistance. It is an important means of comprehensive control.

The composition of the present invention uses cyprodinil (A) as an active ingredient and a triazole fungicide (B) as another active ingredient, and the combination of them is effective against diseases such as rice blast, powdery mildew, gray mold, etc. There is an obvious synergistic effect, not just a simple additive effect of the two agents, which is evident from the results of the following bioassay examples.

Bioassay example 1 Toxicity determination of cyprodinil (A) and a triazole fungicide (B) compounded against cucumber powdery mildew

Test object: Cucumber powdery mildew

The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY/T1156.2-2006" This test uses the cucumber powdery mildew pot test method. On the basis of the preliminary test, select 2 melon seedlings at the true leaf stage with the same growth, write the label number with an oily marker, insert it into the pot of cucumber seedlings, discharge them in sequence, and spray the medicine on the melons by spraying the leaves. On the leaves of seedlings, after spraying treatment, dry the test materials in the fume hood or greenhouse for 24 hours before inoculation. Cultivate in the greenhouse for 7 days to investigate the control effect, the grading standard implements the national standard of the People's Republic of China "Pesticide Field Efficacy Test Guidelines (1)", calculate the control effect with the disease index, use DPS data processing software for statistical analysis, and calculate the EC of each agent ₅₀ , and then calculate the co-toxicity coefficient (CTC) according to Sun Yunpei's method.

When CTC≤80, the composition exhibits antagonistic effect; when 80<CTC<120, the composition exhibits additive effect; when CTC≥120, the composition exhibits synergistic effect.

Actual toxicity index (ATI) = (standard drug EC ₅₀ / test drug EC ₅₀ ) × 100

Theoretical toxicity index (TTI) = Toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture

Co-toxicity coefficient (CTC) = [mixture measured toxicity index (ATI) / mixture theoretical toxicity index (TTI)] × 100

See Table 1 for the results of the combined assay of cyprodinil and difenoconazole, and see Table 2 for the combined assay results of cyprodinil and trifenoconazole.

The series ratio of the composition of table 1 is to the indoor toxicity test result of cucumber powdery mildew

deal with _EC50 (μg/mL) ATI TTI Co-toxicity coefficient CTC Cyprodinil 8.12 100 / / Difenoconazole 15.02 54.1 / / Cyprodinil 50: Difenoconazole 1 5.07 160.2 99.1 161.6 Cyprodinil 20: Difenoconazole 1 4.62 175.8 97.8 179.7 Cyprodinil 10: Difenoconazole 1 3.85 210.9 95.8 220.1 Cyprodinil 5: Difenoconazole 1 2.22 365.8 92.3 396.1 Cyprodinil 1: Difenoconazole 1 2.57 316.0 77.0 410.2 Cyprodinil 1: Difenoconazole 5 3.28 247.6 61.7 401.1 Cyprodinil 1: Difenoconazole 20 5.94 136.7 56.2 243.0 Cyprodinil 1: Difenoconazole 50 8.72 93.1 55.0 169.4

The test results show that the combination of cyprodinil and difenoconazole can prevent and control cucumber powdery mildew. When the ratio is between 50:1-1:50, the co-toxicity coefficient CTC is above 120, which has obvious synergistic effect. And between 20:1-1:20, the co-toxicity coefficients of listed ratios are all higher than 179.7, and the synergistic effect is more obvious.

The series proportioning of table 2 composition is to the indoor toxicity test result of cucumber powdery mildew

deal with _EC50 (μg/mL) ATI TTI Co-toxicity coefficient CTC

Cyprodinil 14.12 100 / / Fenazole 20.15 70.1 / / Cyprodinil 50: Triconazole 1 10.13 139.4 99.4 140.2 Cyprodinil 20: Triconazole 1 9.72 145.3 98.6 147.4 Cyprodinil 10: Triconazole 1 9.52 148.3 97.3 152.5 Cyprodinil 5: Triconazole 1 9.35 151.0 95.0 158.9 Cyprodinil 1: Triconazole 1 8.73 161.7 85.0 190.2 Cyprodinil 1: Triconazole 5 12.74 110.8 75.1 147.7 Cyprodinil 1: Triconazole 20 13.54 104.3 71.5 145.9 Cyprodinil 1: Triconazole 50 15.12 93.4 70.7 132.2

The test results showed that when the combination of cyprodinil and triazol was used to control cucumber powdery mildew, when the ratio was between 50:1-1:50, the co-toxicity coefficient CTC was higher than 120, which had a synergistic effect, while at 20 :1-1:20, the co-toxicity coefficients of listed ratios are all above 145.9, and the synergistic effect is more obvious.

Biological Assay Example 2: Cyprodinil (A) and a triazole fungicide (B) combined toxicity assay for rice blast

Test object: rice blast fungus

The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY/T1156.2-2006". There were five replicates for each concentration, and a total of 10 rice plants were divided into 5 pots for each treatment. Water treatment was used as blank control. Wash the spores on the lower surface of the cultivated pathogenic bacteria with sterile water to prepare a spore suspension of 1×10 ⁶ spores/ml. When the rice is in the three-leaf stage, use a crop sprayer to inoculate it on the rice leaves and evenly spray the inoculation. After 24 hours of darkness, moisturize and remove after 10 days. The number of diseased spots on each rice leaf (30 leaves in total) was counted, and graded according to the number of leaf diseased spots. The disease index was used to calculate the control effect, and the DPS data processing software was used for statistical analysis to calculate the EC ₅₀ of each agent, and then the co-toxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

Grading standards:

Leaf blast (in leaves)

Level 0: no disease;

Grade 1: There are less than 5 lesion spots on the leaves, and the length is less than 1cm;

Grade 3: 6-10 lesions on leaves, some of which are longer than 1cm;

Grade 5: 11-25 lesion spots on leaves, some of which are contiguous into sheets, accounting for 10%-25% of the leaf area;

Level 7: There are more than 26 diseased spots on the leaves, and the diseased spots are connected into sheets, accounting for 26-50% of the leaf area;

Grade 9: Lesions are contiguous into sheets, accounting for more than 50% of the leaf area, or the whole leaf is dead.

Drug efficacy calculation method:

The determination results of cyprodinil combined with hexaconazole and propiconazole respectively against rice blast are shown in Table 3 and Table 4.

Table 3 The indoor toxicity test results of the composition series ratio to rice blast

deal with _EC50 (μg/mL) ATI TTI Co-toxicity coefficient CTC Cyprodinil 9.12 100 / / Hexaconazole 15.02 60.7 / / Cyprodinil 50: Hexaconazole 1 7.07 129.0 99.2 130.0 Cyprodinil 20: Hexaconazole 1 6.12 149.0 98.1 151.9 Cyprodinil 10: Hexaconazole 1 5.85 155.9 96.4 161.7 Cyprodinil 5: Hexaconazole 1 5.67 160.8 93.5 172.1

Cyprodinil 1: Hexaconazole 1 7.37 123.7 80.4 154.0 Cyprodinil 1: Hexaconazole 5 8.08 112.9 67.3 167.8 Cyprodinil 1: Hexaconazole 20 8.94 102.0 62.6 163.0 Cyprodinil 1: Hexaconazole 50 10.42 87.5 61.5 142.3

The test results showed that when the compounding ratio of cyprodinil and hexaconazole was between 50:1 and 1:50 for the control of rice blast, the co-toxicity coefficient CTC was above 130.0, which showed obvious synergistic effect. When the ratio is between 20:1-1:20, the co-toxicity coefficients of the listed ratios are all higher than 151.9, and the synergistic effect is the most obvious.

The series ratio of table 4 composition is to the indoor toxicity test result of rice blast

deal with _EC50 (μg/mL) ATI TTI Co-toxicity coefficient CTC Cyprodinil 7.12 100 / / Propiconazole 13.25 53.7 / / Cyprodinil 50: Propiconazole 1 4.87 146.2 99.1 147.5 Cyprodinil 20: Propiconazole 1 4.22 168.7 97.8 172.5 Cyprodinil 10: Propiconazole 1 4.22 168.7 95.8 176.1 Cyprodinil 5: Propiconazole 1 3.87 184.0 92.3 199.4 Cyprodinil 1: Propiconazole 1 3.42 208.2 76.9 270.8 Cyprodinil 1: Propiconazole 5 4.79 148.6 61.4 241.9 Cyprodinil 1: Propiconazole 20 4.57 155.8 55.9 278.5 Cyprodinil 1: Propiconazole 50 7.92 89.9 54.6 164.5

The test results show that the combination of cyprodinil and propiconazole can control rice blast, and when the ratio is between 50:1-1:50, the co-toxicity coefficient CTC is higher than 147.5, which has obvious synergistic effect, while When it is between 20:1-1:20, the co-toxicity coefficients of the listed ratios are all above 172.5, and the synergistic effect is the most obvious.

Bioassay Example 3: Toxicity determination of cyprodinil (A) and a triazole fungicide (B) compounded against Botrytis cinerea

Test object: Botrytis cinerea of cucumber, Botrytis cinerea of tomato

The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press) and "Pesticide Indoor Bioassay Test Guidelines NY/T1156.2-2006" using the growth rate method (plate method). On the basis of the preliminary test, on the aseptic operation table, quantitatively add the pre-thawed sterilized medium into the sterile Erlenmeyer flask according to the test treatment. Have 45ml of heat medium (PDA medium, 45-50 ℃) in the Erlenmeyer flask, after shaking well, pour into the glass petri dish of diameter 90mm rapidly, each petri dish is poured into 10ml of culture medium with medicine. Set aside horizontally to cool and form into slabs. Each concentration was replicated five times. The treatment without the active ingredient of the drug was used as the blank control. With the cultivated pathogenic bacteria, under aseptic conditions, use a sterilized hole puncher with a diameter of 4mm to cut the bacteria cake from the edge of the colony, inoculate the bacteria cake in the center of the drug-containing plate with an inoculator, and put the side with mycelium down and The culture medium fits well, and the lid of the dish is covered. All of the above operations were performed aseptically on an ultra-clean bench. After treatment, place them in a constant temperature sterile incubator at 26±0.5°C for cultivation, and take them out after 5 days. The colony diameter (in millimeters) of each treatment was measured by the cross method, and the average value of the colony diameter was calculated. DPS data processing software was used for statistical analysis to calculate the EC ₅₀ of each drug, and then the co-toxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

See Table 5 for the determination results of the combination of cyprodinil and penconazole against Botrytis cinerea, and Table 6 for the determination results of the combination of cyprodinil and flusilazole against gray mold.

The series proportioning of table 5 composition is to the indoor toxicity test result of cucumber botrytis

The test results showed that when the compounding ratio of cyprodinil and penconazole was between 50:1 and 1:50, the co-toxicity coefficient CTC of the listed ratio was above 146.6, which showed a significant increase. Effect, while between 20:1-1:20, the co-toxicity coefficients of listed ratios are all higher than 219.4, and the synergistic effect is the most obvious.

The series proportioning of table 6 composition is to the indoor toxicity test result of tomato botrytis cinerea

deal with _EC50 (μg/mL) ATI TTI Co-toxicity coefficient CTC Cyprodinil 0.82 100 / / Flusilazole 1.22 67.2 / / Cyprodinil 50: Flusilazole 1 0.58 141.4 99.4 142.3 Cyprodinil 20: Flusilazole 1 0.53 154.7 98.4 157.2 Cyprodinil 10: Flusilazole 1 0.51 160.8 97.0 165.7 Cyprodinil 5: Flusilazole 1 0.49 167.3 94.5 177.0 Cyprodinil 1: Flusilazole 1 0.47 174.5 83.6 208.7 Cyprodinil 1: Flusilazole 5 0.58 141.4 72.7 194.5 Cyprodinil 1: Flusilazole 20 0.64 128.1 68.8 186.3 Cyprodinil 1: Flusilazole 50 0.82 100.0 67.9 147.4

The test results showed that when the compounding ratio of cyprodinil and flusilazole was between 50:1-1:50, the co-toxicity coefficient CTC of the listed ratios were all higher than 142.3, which had obvious effect on the prevention and control of gray mold of tomato. synergistic effect, and between 20:1-1:20, the co-toxicity coefficients of the listed ratios are all above 157.2, and the synergistic effect is the most obvious.

By measuring on various objects, the results all show that when cyprodinil (A) is compounded with a selected triazole fungicide (B) at a ratio of 50:1-1:50, It has a synergistic effect, and the synergistic effect is more obvious when the ratio is between 20:1-1:20, and the co-toxicity coefficient is above 145.9.

The fungicidal composition of the present invention can be prepared into wettable powders, suspension concentrates, emulsifiable concentrates, water emulsions, microemulsions or water dispersible granules suitable for agricultural use by known methods. Illustrate below with specific embodiment, percentage is mass percent in formula.

Example 1: 51% Cyprodinil Difenoconazole Suspension Concentrate

Cyprodinil 50%

Difenoconazole 1%

Sodium methylnaphthalenesulfonate formaldehyde condensate (dispersant) 10%

Xanthan gum (thickener) 1%

Bentonite (carrier) 1%

Glycerol (antifreeze) 1%

Water to make up to 100%.

Mix the active ingredients (in this application document, cyprodinil and triazole fungicides, which will not be described in detail below), the original drug, dispersant, wetting agent and water according to the proportion of the formula, and grind and/or Or obtain 51% cyprodinil·difenoconazole suspension concentrate after high-speed shearing.

This embodiment is applied to the control of cucumber powdery mildew. Dilute and spray 51% cyprodinil·difenoconazole suspension concentrate 2800 times (the effective concentration of cyprodinil is 178.6g/ml, and the effective concentration of difenoconazole is 3.6 μg/ml) with water, The control effects of 7 days and 15 days were 96.5% and 94.2% respectively. 50% cyprodinil suspension was used 2000 times (effective concentration is 250 μg/ml) and 5% difenoconazole emulsifiable concentrate was 1000 times (effective concentration was 50 μg/ml), used in the same way, after 7 days and The 15-day control effects were 91.1%, 84.9%, 87.9%, 83.6%, respectively. The combination of cyprodinil and difenoconazole has obvious synergistic effect, and the control effect on cucumber powdery mildew is obviously better than that of single agent.

Embodiment 2: 20% cyprodinil·hexaconazole emulsion in water

Cyprodinil 15%

Hexaconazole 5%

N-Methylpyrrolidone (Solvent) 10%

Calcium dodecylbenzenesulfonate (emulsifier) 5%

Nongru 600# (emulsifier) 5%

Water to make up to 100%

Add the original drug (the original drug in this application document refers to the chemically synthesized, unprocessed active ingredient, which will not be described in detail below), solvent, and emulsifier to dissolve into a uniform oil phase; mix the water-soluble components with water to prepare Obtain an aqueous phase; under high-speed stirring, mix the oil phase and the aqueous phase to obtain a 20% cyprodinil·hexaconazole emulsion in water.

This embodiment is applied to the prevention and treatment of rice blast. Dilute 20% cyprodinil·hexaconazole aqueous emulsion 1500 times (the effective concentration of cyprodinil is 100 μg/ml, and the effective concentration of hexaconazole is 33.3 μg/ml) and spray it with water, 7 days and 15 days after the drug The control effects were 89.7% and 92.9% respectively. 20% cyprodinil suspension was used by 1500 times (effective concentration is 133.3 μg/ml) and 10% hexaconazole suspension by 1000 times (effective concentration was 100 μg/ml), used in the same way, after 7 days and The 15-day control effects were 86.3%, 87.2%, 82.8%, 83.4%, respectively. The combination of cyprodinil and hexaconazole has obvious synergistic effect, and the control effect on rice blast is obviously better than that of single agent.

Embodiment 3: 5% cyprodinil · penconazole microemulsion

Cyprodinil 2.5%

Penconazole 2.5%

N-Methylpyrrolidone (Solvent) 10%

Isopropanol (co-solvent) 15%

Agricultural milk 500# (emulsifier) 5%

Nongru 1601# (Emulsifier) 5%

Water to make up to 100%.

Add the original drug, solvent and emulsifier together to dissolve into a uniform oil phase; mix the water-soluble components with water to obtain a water phase; mix the oil phase with the water phase under high-speed stirring to obtain a 5% pyrimidine Cyprodinil penconazole microemulsion.

This embodiment is applied to the prevention and treatment of gray mold of cucumber. Dilute and spray 5% cyprodinil·pentconazole microemulsion 500 times (the effective concentration of cyprodinil is 50 μg/ml, and the effective concentration of penconazole is 50 μg/ml) with water. The control effects were 85.2% and 82.3% respectively. 5% cyprodinil suspension was used 500 times (effective concentration is 100 μg/ml) and 5% penconazole suspension was 500 times (effective concentration was 100 μg/ml). The control effects of days were 82.4%, 80.9%, and 80.1%, 79.8%, respectively. The combination of cyprodinil and penconazole has obvious synergistic effect, and the control effect on cucumber gray mold is obviously better than that of single agent.

Example 4: 31% cyprodinil · flusilazole wettable powder

Cyprodinil 1%

Flusilazole 30%

Sodium lauryl sulfate (wetting agent) 2%

Sodium lignosulfonate (dispersant) 5%

Naphthalene Sulfonate (Dispersant) 3%

Kaolin (Filler) Make up to 100%.

The active ingredient, various additives and fillers are fully mixed according to the proportion of the formula, and after being pulverized by a superfine pulverizer, a 31% cyprodinil-flusilazole wettable powder is obtained.

This embodiment is applied to pear scab. Dilute 31% cyprodinil · flusilazole wettable powder by 1500 times (the effective concentration of cyprodinil is 6.7 μg/ml, and the effective concentration of flusilazole is 200 μg/ml) and spray it with water. The control effects of days were 95.3% and 97.7%. 5% Cyprodinil Suspension Concentrate 1000 times (effective concentration 50 μg/ml) and 30% flusilazole EC 1000 times (effective concentration 300 μg/ml), use the same method, 7 days and 15 days after the drug The control effects of the two were 72.9%, 73.9% and 85.9%, 90.5%, respectively. The combination of cyprodinil and flusilazole has obvious synergistic effect, and the control effect on pear scab is obviously better than that of single agent.

Example 5: 41% cyprodinil · propiconazole EC

Cyprodinil 1%

Propiconazole 40%

N-Methylpyrrolidone (Solvent) 10%

DMF (Solvent) 10%

Nongru 1601# (emulsifier) 5%

Agricultural milk 500# (emulsifier) 5%

Xylene (solvent) Make up to 100%.

The active ingredient, emulsifier and auxiliary agent are sequentially added into the mixing kettle according to the proportion of the formula, and stirred evenly to prepare 41% cyprodinil·propiconazole emulsifiable concentrate.

This example is applied to powdery mildew of zucchini. Dilute 41% cyprodinil·propiconazole EC 3000 times (the effective concentration of cyprodinil is 3.3 μg/ml, and the effective concentration of propiconazole is 133.3 μg/ml) and spray it with water, 7 days and 15 days after treatment The control effects were 93.9% and 90.7%, respectively. 5% cyprodinil suspension was used at 600 times (effective concentration of 83.3 μg/ml) and 40% propiconazole EC was used at 2000 times (effective concentration of 200 μg/ml) in the same way. The control effects of days were 75.9%, 73.7% and 85.8%, 83.6%. The combination of cyprodinil and propiconazole has obvious synergistic effect, and the control effect on zucchini powdery mildew is obviously better than that of single agent.

Example 6: 80% cyprodinil·triconazole water dispersible granules

Cyprodinil 30%

Triadol 50%

Sodium alkylnaphthalene sulfonate (dispersant) 4%

Sodium lignosulfonate (dispersant) 2%

Sodium lauryl sulfate (wetting agent) 5%

Kaolin (filler) to 100%

The active ingredient, dispersant, wetting agent, disintegrating agent and filler are mixed evenly according to the ratio of the formula, pulverized into a wettable powder by airflow, and then a certain amount of water is added to mix and extrude the material. After drying and sieving, 80% cyprodinil·triconazole water-dispersible granules were obtained.

This embodiment is applied to the prevention and treatment of wheat powdery mildew. Dilute 80% cyprodinil·fluconazole water dispersible granules 4000 times (the effective concentration of cyprodinil is 75 μg/ml, and the effective concentration of cyprodinil is 125 μg/ml) and spray it with water, 7 days and 15 days after the medicine The control effects were 97.9%, 94.7%. 30% cyprodinil water-dispersible granules are used in the same way by 2000 times (effective concentration is 150 μg/ml) and 50% flutriazol water-dispersible granules are used in the same way (effective concentration is 200 μg/ml) The control effects of 7 days and 15 days were 86.5%, 84.9%, 85.6%, 83.4%, respectively. The synergistic effect of the combination of cyprodinil and triazol is obvious, and the control effect on wheat powdery mildew is obviously better than that of single agent.

Example 7: 30% cyprodinil·difenoconazole water dispersible granules

Cyprodinil 25%

Difenoconazole 5%

Sodium alkylnaphthalene sulfonate (dispersant) 4%

Sodium lignosulfonate (dispersant) 2%

Sodium lauryl sulfate (wetting agent) 5%

Kaolin (filler) to 100%

The active ingredient, dispersant, wetting agent, disintegrating agent and filler are mixed evenly according to the ratio of the formula, pulverized into a wettable powder by airflow, and then a certain amount of water is added to mix and extrude the material. After drying and sieving, 30% cyprodinil·difenoconazole water-dispersible granules were obtained.

This embodiment is applied to the prevention and treatment of wheat powdery mildew. Dilute and spray 30% cyprodinil·difenoconazole water dispersible granules 2000 times (the effective concentration of cyprodinil is 125 μg/ml, and the effective concentration of difenoconazole is 25 μg/ml) with water. 98.9% and 97.7% for 15 days and 15 days respectively. 30% cyprodinil water-dispersible granules are used in the same way by 2000 times (effective concentration is 150 μg/ml) and 10% difenoconazole water-dispersible granules are used by 2000 times (effective concentration is 50 μg/ml), The control effects were 89.5%, 87.9%, 82.6%, 80.4% after 7 days and 15 days after the medicine. The combination of cyprodinil and difenoconazole has obvious synergistic effect, and the control effect on wheat powdery mildew is obviously better than that of single agent.

Claims (8)

1. A bactericidal composition, characterized in that: the active ingredients in the bactericidal composition are cyprodinil (A) and a triazole bactericide (B), and (B) is difenoconazole, Hexaconazole, propiconazole, flusilazole, penconazole, triadimefon, diniconazole, fluconazole or fuconazole, the mass ratio of (A) to (B) is 50:1-1:50 . 2. According to claim 1, characterized in that the mass ratio of active ingredients (A) to (B) is 20:1-1:20. 3. According to claim 1 or 2, it is characterized in that: said fungicidal composition also contains auxiliary components of pesticide preparations, so as to make a dosage form suitable for agricultural use. 4. According to claim 3, characterized in that the suitable dosage form is wettable powder, emulsifiable concentrate, suspension concentrate, water emulsion, microemulsion or water dispersible granule. 5. According to claim 3, it is characterized in that: the total mass percentage of active ingredients (A) and (B) in the bactericidal composition is 5%-80%. 6. The fungicidal composition of claim 1, used in the control of crop diseases. 7. The application of the fungicidal composition according to claim 6, characterized in that: the crops are rice, fruit trees, cotton, vegetables. 8. According to claim 6 or 7, it is characterized in that: said bactericidal composition is used to control fungal diseases.