CN101642032B - Determining method of reasonable illuminating dose of facility vegetable - Google Patents
Determining method of reasonable illuminating dose of facility vegetable Download PDFInfo
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- CN101642032B CN101642032B CN2009100347938A CN200910034793A CN101642032B CN 101642032 B CN101642032 B CN 101642032B CN 2009100347938 A CN2009100347938 A CN 2009100347938A CN 200910034793 A CN200910034793 A CN 200910034793A CN 101642032 B CN101642032 B CN 101642032B
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- transfer rate
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Abstract
The invention discloses a determining method of reasonable illuminating dose of a facility vegetable, which belongs to the technical field of facility growth. The reasonable illuminating dose of the facility vegetable is determined according to the following steps: taking the facility vegetable in the peak of a growing season; measuring a quick light response curve of vegetable leaves by a chlorophyll luminoscope and determining the reasonable illuminating dose of the facility vegetable according to a response curve of the transmission rate of apparent electrons to photosynthetic effective radiation in the quick light response curve of the vegetable leaves. By using the reasonable illuminating dose of the facility vegetable determined by the method, higher yield and lower nitrate accumulation level are ensured and energy sources are saved to the maximum, thereby the unification of high yield, good quality and low consumption is realized. The method has the advantages of high efficiency and speed, convenient operation and wide application range and can be used for facility growth vegetables and facility growth medicaments.
Description
Technical field
The invention belongs to facility culture technical field, be specifically related to a kind of determining method of reasonable illuminating dose of facility vegetable.
Background technology
At present, the production of vegetables facility cultivation is extensively carried out in worldwide.By 2003, domestic facilities horticulture area under cultivation surpassed 1,400,000 hectares, ranks first in the world, and according to scholarly forecast, national horticultural facility area will reach about 2,500,000 hectares by 2010.The vegetables facility cultivation has become one of main means of vegetables production.But, because the facility covering, skeleton structure is sheltered from heat or light and winter-spring season wet weather, factor such as snow weather etc. make crop in the facility often be in low light environment to grow down.Because nitrate excessive buildup in the illumination deficiency, leaf vegetables body, edible this vegetables are totally unfavorable to health.
Light not only provides reducing power and carbon skeleton by photosynthesis for nitrate reductase, activates nitrate reductase but also transmit stream by photosynthetic electronics.Artificial light filling becomes vegetables and produces raising output, reduces the important means of nitrate content.But under the different light intensity, the utilization ratio of light is different.Under low light intensity, optical energy utilization efficiency increases consumingly with the increase of light intensity, and optical energy utilization efficiency is under high light intensity, with the increase of light intensity as long as faintly increase.Applying of nitrate reductase ability and illumination is also disproportionate.Therefore desirable light intensity be the leaf vegetables of high yield and high quality in winter (protonitrate accumulation) low (energy) consumption produce the key factor of necessary consideration.The present invention utilizes the chlorophyll fluorescence technology to determine reasonable illuminating dose of facility vegetable exactly, for artificial light filling provides technical parameter.
Summary of the invention
The objective of the invention is to overcome the deficiency that present reasonable illuminating dose of facility vegetable neither one is objectively determined method, disclose a kind of determining method of reasonable illuminating dose of facility vegetable.
Technical scheme of the present invention is: the facilities vegetable of getting peak of growing season, utilize the chlorophyll fluorescence instrument, measure the quick photoresponse curve of vegetable leaf, the response curve of photosynthetic active radiation followed these steps to determine reasonable illuminating dose of facility vegetable according to the apparent electronics transfer rate in the quick photoresponse curve of vegetable leaf:
(1) calculates actual photosynthesis electronics transfer rate.Find out under low photosynthetic active radiation, straight line portion in apparent electronics transfer rate and the photosynthetic active radiation curve, utilize statistical software to obtain the linear equation Y=aX of this part, wherein a is a constant, the initial slope of representing apparent electronics transfer rate and photosynthetic active radiation curve, Y is apparent electronics transfer rate, and X is photosynthetic active radiation (μ mol m
-2s
-1).Obtain electronics transfer rate under each photosynthetic active radiation according to this linear equation, this electronics transfer rate is defined as actual photosynthesis electronics transfer rate.
(2) calculate the photosynthetic electronics transmission stream of residue percentage under each photosynthetic active radiation.The difference that to remain photosynthetic electronics transfer rate be actual photosynthesis electronics transfer rate and apparent electronics transfer rate.Remain photosynthetic electronics transmission stream percentage and remain the percentage that photosynthetic electronics transfer rate accounts for actual photosynthesis electronics transfer rate down for each photosynthetic active radiation.
(3) obtaining the photosynthetic electronics transmission of residue stream percentage is 8% o'clock photosynthetic active radiation.Utilize interpolation method to calculate the photosynthetic electronics transmission of residue stream percentage 8% o'clock photosynthetic active radiation value, this photosynthetic active radiation value is exactly the predicted value that is applied to the reasonable light intensity on the facilities vegetable.
(4) determine reasonable illuminating dose of facility vegetable.Because apparent photosynthetic electronics transfer rate has certain variation amplitude on plant leaf blade, therefore, employed illuminating dose should can guarantee that just nitrate significantly descends than above-mentioned predicted value is big in the production.Go sightseeing to close electronics transfer rate value variation amplitude according to blade table,, therefore, determine that reasonable illuminating dose of facility vegetable is the 115% more suitable of predicted value in conjunction with experimental result repeatedly generally below 15%.Also promptly: reasonable illuminating dose of facility vegetable for the photosynthetic electronics transmission of residue stream percentage in 115% of 8% o'clock photosynthetic active radiation value.
Basic principle of the present invention is: under low light intensity, the photosynthetic electronics transmission stream of vegetable leaf only is used to the reduction of carbon, and nitrate reductase speed is slower.Under high light intensity, the photosynthetic electronics transmission stream of vegetable leaf not only is used to the reduction of carbon, also is used to the reduction of nitric acid.Have only when photosynthetic electronics transmission stream and reach the switch that 8% electronics transmission stream just can be opened nitrate reductase satisfying to have more on the basis of carbon reduction, nitrate reductase speed is increased sharply, the illumination that vegetable leaf applied of this moment is a rational light intensity, can reach the unification that improves output, increases quality (nitrates accumulation decline), energy savings.
Advantage of the present invention:
(1) applies the reasonable illuminating dose of facility vegetable that this method determines and both can guarantee higher output, lower nitrates accumulation level, energy savings to greatest extent again.Reach the unification of high yield, high quality and low cost.
(2) efficient, quick, easy to operate.
(3) range of application is wider.Can be used for facility cultivation vegetables and facility plantation traditional Chinese medicine.
Description of drawings
Fig. 1 is apparent electronics transfer rate of the romaine lettuce of embodiment 1 and the quick photoresponse curve of photosynthetic active radiation;
Fig. 2 is apparent electronics transfer rate of the Orychophragmus violaceus of embodiment 2 and the quick photoresponse curve of photosynthetic active radiation;
Fig. 3 is apparent electronics transfer rate of the Chinese cabbage of embodiment 3 and the quick photoresponse curve of photosynthetic active radiation.
Embodiment
Embodiment 1
30 days greenhouse romaine lettuce (Lactuca sativa L.) (kind: the Italian romaine lettuce of anti-the bolting) behind the taking-up seedling.Measure the quick photoresponse curve of Chinese leaf with PAM-2000 modulation system chlorophyll fluorescence instrument.The apparent electronics transfer rate of foundation follows these steps to determine reasonable illuminating dose to the response curve (Fig. 1) of photosynthetic active radiation.
Step 1 is calculated actual photosynthesis electronics transfer rate.Find out under low photosynthetic active radiation, straight line portion in apparent electronics transfer rate and the photosynthetic active radiation curve, utilize statistical software to obtain the linear equation Y=aX (a is a constant, represents the initial slope of apparent electronics transfer rate and photosynthetic active radiation curve) of this part.This equation is:
Y=0.252X (R
2=0.997, n=6, P＜0.001, X is less than 134 μ mol m
-2s
-1) (1)
Y is apparent electronics transfer rate, and X is photosynthetic active radiation (μ mol m
-2s
-1), R
2For determination coefficient square, n for the statistics number, P is a significance.
Obtain actual photosynthesis electronics transfer rate (table 1) under each photosynthetic active radiation according to this linear equation.
The photosynthetic electronics transmission stream of the actual photosynthesis electronics transfer rate of table 1 embodiment 1 and residue percentage
Step 2 is calculated each photosynthetic active radiation photosynthetic electronics transmission stream of residue percentage down.Calculate the difference of actual photosynthesis electronics transfer rate and apparent electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transfer rate of residue.Calculate and remain the percentage that photosynthetic electronics transfer rate accounts for actual photosynthesis electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transmission stream of residue percentage (table 1).
It is 8% o'clock photosynthetic active radiation that step 3 is obtained the photosynthetic electronics transmission of residue stream percentage.Utilize interpolation calculation to remain photosynthetic electronics transmission stream percentage 8% o'clock photosynthetic active radiation value, draw the predicted value that is applied to the reasonable light intensity on the facilities vegetable, the result is 177 μ mol m
-2s
-1
Step 4 is determined reasonable illuminating dose of facility vegetable.The predicted value of above-mentioned reasonable illuminating dose of facility vegetable be multiply by 115%, be reasonable illuminating dose of facility vegetable, the result is 204 μ mol m
-2s
-1
Embodiment 2
45 days greenhouse Orychophragmus violaceus (Orychophragmus violaceus L.) behind the taking-up seedling.Measure the quick photoresponse curve of Orychophragmus violaceus with PAM-2000 modulation system chlorophyll fluorescence instrument.According to the response curve (Fig. 2) of apparent electronics transfer rate, follow these steps to determine reasonable illuminating dose to photosynthetic active radiation.
Step 1 is calculated actual photosynthesis electronics transfer rate.Find out under low photosynthetic active radiation, straight line portion in apparent electronics transfer rate and the photosynthetic active radiation curve, utilize statistical software to obtain the linear equation Y=aX (a is a constant, represents the initial slope of apparent electronics transfer rate and photosynthetic active radiation curve) of this part.This equation is:
Y=0.189X (R
2=0.998, n=10, P＜0.001, X is less than 456 μ mol m
-2s
-1) (2)
Y is apparent electronics transfer rate, and X is photosynthetic active radiation (μ mol m
-2s
-1), R
2For determination coefficient square, n for the statistics number, P is a significance.
Obtain actual photosynthesis electronics transfer rate (table 2) under each photosynthetic active radiation according to this linear equation.
The photosynthetic electronics transmission stream of the actual photosynthesis electronics transfer rate of table 2 embodiment 2 and residue percentage
Step 2 is calculated each photosynthetic active radiation photosynthetic electronics transmission stream of residue percentage down.Calculate the difference of actual photosynthesis electronics transfer rate and apparent electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transfer rate of residue.Calculate and remain the percentage that photosynthetic electronics transfer rate accounts for actual photosynthesis electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transmission stream of residue percentage (table 2).
It is 8% o'clock photosynthetic active radiation that step 3 is obtained the photosynthetic electronics transmission of residue stream percentage.Utilize interpolation calculation to remain photosynthetic electronics transmission stream percentage 8% o'clock photosynthetic active radiation value, draw the predicted value that is applied to the reasonable light intensity on the facilities vegetable, the result is 537 μ mol m
-2s
-1
Step 4 is determined reasonable illuminating dose of facility vegetable.The predicted value of above-mentioned reasonable illuminating dose of facility vegetable be multiply by 115%, be reasonable illuminating dose of facility vegetable, the result is 618 μ mol m
-2s
-1
Embodiment 3
45 days greenhouse Chinese cabbage (Brassica rapa L.ssp.pekinensis) behind the taking-up seedling.Measure the quick photoresponse curve of Chinese cabbage with PAM-2000 modulation system chlorophyll fluorescence instrument.The apparent electronics transfer rate of foundation follows these steps to determine reasonable illuminating dose to the response curve (Fig. 3) of photosynthetic active radiation.
Step 1 is calculated actual photosynthesis electronics transfer rate.Find out under low photosynthetic active radiation, straight line portion in apparent electronics transfer rate and the photosynthetic active radiation curve, utilize statistical software to obtain the linear equation Y=aX (a is a constant, represents the initial slope of apparent electronics transfer rate and photosynthetic active radiation curve) of this part.This equation is:
Y=0.223X (R
2=0.997, n=7, P＜0.001, X is less than 249 μ mol m
-2s
-1) (3)
Y is apparent electronics transfer rate, and X is photosynthetic active radiation (μ mol m
-2s
-1), R
2For determination coefficient square, n for the statistics number, P is a significance.
Obtain actual photosynthesis electronics transfer rate (table 3) under each photosynthetic active radiation according to this linear equation.
The photosynthetic electronics transmission stream of the actual photosynthesis electronics transfer rate of table 3 embodiment 3 and residue percentage
Step 2 is calculated each photosynthetic active radiation photosynthetic electronics transmission stream of residue percentage down.Calculate the difference of actual photosynthesis electronics transfer rate and apparent electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transfer rate of residue.Calculate and remain the percentage that photosynthetic electronics transfer rate accounts for actual photosynthesis electronics transfer rate under each photosynthetic active radiation, draw the photosynthetic electronics transmission stream of residue percentage (table 3).
It is 8% o'clock photosynthetic active radiation that step 3 is obtained the photosynthetic electronics transmission of residue stream percentage.Utilize interpolation calculation to remain photosynthetic electronics transmission stream percentage 8% o'clock photosynthetic active radiation value, draw the predicted value that is applied to the reasonable light intensity on the facilities vegetable, the result is 275 μ mol m
-2s
-1
Step 4 is determined reasonable illuminating dose of facility vegetable.The predicted value of above-mentioned reasonable illuminating dose of facility vegetable be multiply by 115%, be reasonable illuminating dose of facility vegetable, the result is 316 μ mol m
-2s
-1
The implementation result of each embodiment
In order to check the effect of embodiment, in romaine lettuce (the Italian romaine lettuce of anti-the bolting), Orychophragmus violaceus and Chinese cabbage cultivation in the controlled environment chamber, apply different light intensity and cultivate.The artificial climate room environmental is controlled to be: 25 ℃/20 ℃ of temperature (day/night), and illumination every day 16 hours dark 8 hours, was handled for two weeks under such intensity gradient; Humidity is constant humidity 60%, because of indoor gas concentration lwevel maintains this level of 450-600ppm basically.In the experiment, water in right amount according to doing wet situation.Each is handled and places 10 strain seedlings, triplicate.After the processing, measure each Net Photosynthetic Rate handled and the nitrate content in the vegetables blade.Result such as table 4.
Table 4 romaine lettuce (the Italian romaine lettuce of anti-the bolting), Orychophragmus violaceus and Chinese cabbage are cultivated the content at different light intensity lower blade Net Photosynthetic Rate and nitrate
As can be seen from Table 4, romaine lettuce is from 70 μ mol m
-2s
-1Increase to 145 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 1.12 μ mol m
-2s
-1, nitrate has reduced 6090mg.g
-1FW is from 145 μ mol m
-2s
-1Increase to 210 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 1.40 μ mol m
-2s
-1, nitrate has reduced 4680mg.g
-1FW is from 210 μ mol m
-2s
-1Increase to 285 μ mol m
-2s
-1The time, Net Photosynthetic Rate has only increased by 0.52 μ mol m
-2s
-1, nitrate has only reduced 200mg.g
-1FW is from 285 μ mol m
-2s
-1Increase to 360 μ mol m
-2s
-1The time, Net Photosynthetic Rate has only increased by 0.31 μ mol m
-2s
-1, nitrate has only reduced 85mg.g
-1FW.Obviously, at 210 μ mol m
-2s
-1Intensity of illumination under, Net Photosynthetic Rate increases and the nitrate reduction has a flex point.Simultaneously, Ci Shi blade nitrate content has been lower than 3000mg.g
-1FW reaches the standard of pot-herb.So 210 μ mol m
-2s
-1Intensity of illumination be illuminating dose best in this experiment.204 μ mol m in this and the example 1
-2s
-1Intensity of illumination more close, show 204 μ mol m
-2s
-1Intensity of illumination be believable as greenhouse romaine lettuce reasonable illuminating dose.Verified the effect of example 1.
As can be seen from Table 4, Orychophragmus violaceus is from 245 μ mol m
-2s
-1Increase to 370 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 3.45 μ mol m
-2s
-1, nitrate has increased 230mg.g
-1FW is from 370 μ mol m
-2s
-1Increase to 495 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 3.15 μ mol m
-2s
-1, nitrate has reduced 290mg.g
-1FW is from 495 μ mol m
-2s
-1Increase to 620 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 1.89 μ mol m
-2s
-1, nitrate has reduced 980mg.g
-1FW is from 620 μ molm
-2s
-1Increase to 745 μ mol m
-2s
-1The time, Net Photosynthetic Rate has only increased by 0.58 μ mol m
-2.s
-1, nitrate has only reduced 30mg.g
-1FW.Obviously, at 620 μ mol m
-2s
-1Intensity of illumination under, Net Photosynthetic Rate increases and the nitrate reduction has a flex point.Simultaneously, Ci Shi blade nitrate content has only 870mg.g
-1FW.So 620 μ mol m
-2s
-1Intensity of illumination be illuminating dose best in this experiment.618 μ mol m in this and the example 2
-2s
-1Intensity of illumination more close, show 618 μ molm
-2s
-1Intensity of illumination be believable as greenhouse Orychophragmus violaceus reasonable illuminating dose.Verified the effect of example 2.
As can be seen from Table 4, Chinese cabbage is from 120 μ mol m
-2s
-1Increase to 220 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 1.27 μ mol m
-2s
-1, nitrate has reduced 460mg.g
-1FW is from 220 μ mol m
-2s
-1Increase to 320 μ mol m
-2s
-1The time, Net Photosynthetic Rate has increased by 1.19 μ mol m
-2s
-1, nitrate has reduced 1540mg.g
-1FW is from 320 μ mol m
-2.s
-1Increase to 420 μ mol m
-2.s
-1The time, Net Photosynthetic Rate has only increased by 0.58 μ mol m
-2.s
-1, nitrate has only reduced 140mg.g
-1FW is from 420 μ mol m
-2s
-1Increase to 520 μ mol m
-2.s
-1The time, Net Photosynthetic Rate has only increased by 0.48 μ mol m
-2s
-1, nitrate has only reduced 80mg.g
-1FW.Obviously, at 320 μ mol m
-2s
-1Intensity of illumination under, Net Photosynthetic Rate increases and the nitrate reduction has a flex point.Simultaneously, Ci Shi blade nitrate content has been lower than 3000mg.g
-1FW reaches the standard of pot-herb.So 320 μ mol m
-2s
-1Intensity of illumination be illuminating dose best in this experiment.316 μ mol m in this and the example 3
-2s
-1Intensity of illumination more close, show 316 μ mol m
-2s
-1Intensity of illumination be believable as greenhouse Chinese cabbage reasonable illuminating dose.Verified the effect of example 3.
Claims (1)
1. determining method of reasonable illuminating dose of facility vegetable, get the facilities vegetable of peak of growing season, utilize the chlorophyll fluorescence instrument, measure the quick photoresponse curve of vegetable leaf, according to the response curve of the apparent electronics transfer rate in the quick photoresponse curve of vegetable leaf to photosynthetic active radiation, determine reasonable illuminating dose of facility vegetable, it is characterized in that determining reasonable illuminating dose of facility vegetable by following step: (1) calculates actual photosynthesis electronics transfer rate, find out under low photosynthetic active radiation, straight line portion in apparent electronics transfer rate and the photosynthetic active radiation curve, utilize statistical software to obtain the linear equation Y=aX of this part, wherein a is a constant, the initial slope of representing apparent electronics transfer rate and photosynthetic active radiation curve, Y is apparent electronics transfer rate, X is a photosynthetic active radiation; Obtain electronics transfer rate under each photosynthetic active radiation according to this linear equation, be actual photosynthesis electronics transfer rate;
(2) calculate the photosynthetic electronics transmission stream of residue percentage under each photosynthetic active radiation, the difference that to remain photosynthetic electronics transfer rate be actual photosynthesis electronics transfer rate and apparent electronics transfer rate remains photosynthetic electronics transmission stream percentage and remains the percentage that photosynthetic electronics transfer rate accounts for actual photosynthesis electronics transfer rate down for each photosynthetic active radiation;
(3) obtaining the photosynthetic electronics transmission of residue stream percentage is 8% o'clock photosynthetic active radiation, utilize interpolation method to calculate the photosynthetic electronics transmission of residue stream percentage 8% o'clock photosynthetic active radiation value, this photosynthetic active radiation value is exactly the predicted value that is applied to the reasonable light intensity on the facilities vegetable;
(4) determine reasonable illuminating dose of facility vegetable, reasonable illuminating dose of facility vegetable is 115% of the middle predicted value of above-mentioned steps (3).
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| CN102365925B (en) * | 2010-12-29 | 2013-02-27 | 中国科学院地球化学研究所 | Method for determining ability of plant on using nitrate |
| CN102147367A (en) * | 2010-12-29 | 2011-08-10 | 大连海事大学 | Method for detecting crop stress physiology and appraising stress resistance by delayed fluorescence spectrum |
| CN103718846B (en) * | 2013-12-23 | 2015-12-02 | 广西大学 | A kind of LED light source reduces the method for Nitrate |
| CN105766284B (en) * | 2016-03-07 | 2019-04-02 | 江苏大学 | Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group |
| NL2018324B1 (en) * | 2017-02-07 | 2018-09-03 | Priva Holding B V | Method and device for growing a crop |
| CN116171840B (en) * | 2023-01-18 | 2023-09-08 | 广州市林业和园林科学研究院 | Method for regulating flowering phase of bougainvillea |
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