CN105766284B - Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group - Google Patents

Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group Download PDF

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CN105766284B
CN105766284B CN201610128769.0A CN201610128769A CN105766284B CN 105766284 B CN105766284 B CN 105766284B CN 201610128769 A CN201610128769 A CN 201610128769A CN 105766284 B CN105766284 B CN 105766284B
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phalaris
reed
grass
phalaris grass
under
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CN105766284A (en
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付为国
赵云
王凡坤
滕博群
王雨轩
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Jiangsu University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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Abstract

The invention discloses Phalaris grass light in a kind of reed Phalaris grass group to compensate the determination method utilized, belongs to light resources effective utilization field in plant vertical distribution.Include the following steps: that Phalaris is careless under the reed canopy of peak of growing season in selection reed Phalaris grass group, it is fitted using the photoresponse curve that artificial light source carries out Phalaris blade of grass piece, and the intensity of illumination under Phalaris grass surface layer and the unobstructed environment of surrounding is tested respectively, then according to the photoresponse curve of Phalaris grass, calculate Net Photosynthetic Rate and its ratio possessed by Phalaris grass under both the above intensity of illumination, to estimate the light compensation utilization rate of Phalaris grass under reed canopy, and then it can determine the Suitable Density of reed in reed Phalaris grass group.The present invention is simple and easy to do, and data are accurate, stablize, and can be applied to have the light of plant under Characteristics of Vertical Distribution group canopy to compensate the determination utilized.

Description

Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group
Technical field:
The present invention relates to Phalaris grass light in a kind of reed Phalaris grass group to compensate the determination method utilized, belongs to plant vertical distribution Middle smooth resources effective utilization field.
Background technique:
Phalaris grass (Phalaris herba) is distributed mainly on North America, Northern Europe and Asia zone of constant temperature area, in Northeast China, China The ground such as north, northwest and East China are distributed.Phalaris grass is hygrophilous, is frequently grown and overflows side, lakeside, low-lying land and marshland in river.Phalaris grass tool There is the features such as yield is high, and protein, cellulose and content of mineral substances are abundant, is high quality forage.In addition, Phalaris grass and reed although North America is considered as invasive species and is controlled, but in Europe, and the two is but widely used for building people because of high dirt-removing power Work Wetland Communities, the purposes also starts to be utilized in China in recent years.However, the great competition energy of reed and Phalaris grass Power, the two is often by inhibiting other species to be evolved into the excellent population of list of group in natural ecosystems, but the two occupies sometimes It so can flat maintain in certain riverine wetlands, lakeside and for a long time to coexist, this is classical competition row under general Population Level Reprimand theory is difficult to the phenomenon that explaining.Therefore, must be by taking certain ecology strategies between the two, realization competes-plants a colony compromise, thus Maintain competitive coexistence pattern.According to the lower special efficacy of Phalaris grass light compensation point, proposing Phalaris grass can be in reed for recent team of the present invention Realize that light compensation utilizes under canopy, to can be relieved the deduction of competition of the two to light resource, this judgement has also obtained the world Water assists " wastewater treatment " Committee of Experts chairman, Europe Wetland Science man association chairman Jan Vymazal to teach Approve.But the quantification measuring method for compensating utilization rate not yet has been reported that.This technology method will be in conjunction with light distribution under reed canopy The photosynthesis characteristics of feature and Phalaris grass determine that Phalaris grass light compensates utilization efficiency under reed canopy.
As the LI-6400XT of one of most representative instrument of test plants photosynthesis is portable photosynthetic in the world at present Measuring system is acted on, the CO around blade can be effectively controlled during the experiment2Concentration, humidity, temperature, intensity of illumination and leaf chamber All relevant environmental conditions such as temperature, solve problems in photosynthesis field measurement: gas concentration can be suitable In the range of control and variation, to measure response curve;It solves leaf temperature to increase with light application time and be stepped up Problem, while measuring the intensity of illumination of blade surface;Light source is portable and can control intensity of illumination, and independent of natural weather Condition;System is sturdy and durable, can adapt to various environmental conditions;The red blue light source of LED can be in 0-3000 μm of olm-2·s-1 Between consecutive variations, and be nearly free from heat, disturbance will not be generated to blade.The above plurality of advantages, it can be ensured that this technology method Middle test data is accurate, stablizes.
Summary of the invention:
The purpose of the present invention is to provide Phalaris grass light in a kind of reed Phalaris grass group to compensate the determination method utilized, with determination Phalaris grass realizes the method that light resource compensation utilizes, while also can determine other with plant under Characteristics of Vertical Distribution group canopy Light compensation utilizes method.
Technical solution: in order to achieve the above object, the present invention mainly takes following steps:
(1) density for selecting peak of growing season in reed Phalaris grass group is 25-45 plants/m2Reed canopy under Phalaris grass, one In it in main light application time (8:00-16:00), the piece photoresponse of Phalaris blade of grass is carried out using the portable photosynthetic instrument of LI-6400XT Curve matching, wherein measurement light when measurement is set by endoprosthetic's light source that instrument carries, and sets intensity of illumination range as 0- 2500μmol m-2s-1, system automatically records the Net Photosynthetic Rate of Phalaris blade of grass piece every 3min, and the photoresponse for completing blade is bent Line fitting.
(2) density for selecting peak of growing season in reed Phalaris grass group is 25-45 plants/m2Reed canopy under Phalaris grass, utilize Biradial instrument is under natural lighting, and the different time points Phalaris grass surface layer blade and surrounding in determination step (1) the mentioned period are without screening Intensity of illumination under baffle ring border.
(3) photoresponse curve according to the Phalaris blade of grass piece being fitted in step (1), when calculating each under natural light in step (2) Between intensity of illumination under point Phalaris grass surface layer blade and the unobstructed environment of surrounding Net Photosynthetic Rate corresponding on photoresponse curve.
(4) each time point Phalaris grass surface layer net photosynthetic rate and the unobstructed ring of surrounding under natural light are calculated in step (3) The ratio of Net Photosynthetic Rate is spent under border, to estimate the light compensation utilization rate of Phalaris grass under reed canopy, and then determines reed Phalaris The Suitable Density of reed in group coexists in grass.
Beneficial effects of the present invention:
1. providing Phalaris grass light in a kind of reed Phalaris grass group compensates the determination method utilized, to disclosing Phalaris grass-reed people Competitive coexistence mechanism both in work wetland is of great significance.
It is of great significance 2. pair other determining light compensation with plant under Characteristics of Vertical Distribution group canopy utilize.
Detailed description of the invention
Fig. 1 is the photoresponse curve of Phalaris grass of the present invention surface layer blade morning 8:00 Net Photosynthetic Rate.
Fig. 2 is the photoresponse curve of Phalaris grass of the present invention surface layer blade morning 10:00 Net Photosynthetic Rate.
Fig. 3 is the photoresponse curve of Phalaris grass of the present invention surface layer blade noon 12:00 Net Photosynthetic Rate.
Fig. 4 is the photoresponse curve of Phalaris grass of the present invention surface layer blade 14:00 in afternoon Net Photosynthetic Rate.
Fig. 5 is the photoresponse curve of Phalaris grass of the present invention surface layer blade 16:00 in afternoon Net Photosynthetic Rate.
Fig. 6 is Phalaris grass of the present invention and reed plant height and the disparity map of breeding time.
Specific embodiment
Embodiment 1:
On May 5th, 2015, fair weather select reed Phalaris grass group, and sample area is 1m × 1m, and the height of reed is 2.0m or so, density are 25 plants/m2, the height of Phalaris grass is 1.5m or so.Follow these steps to determine Phalaris grass in reed Phalaris grass group Light compensates the method utilized.
(1) the portable photosynthetic instrument of LI-6400 of U.S. LI-COR company production, the following third piece expansion in measurement top are utilized Leaf central part.Utilize the artificial light source of instrument configuration, time 8:00,10:00,12:00,14:00,16:00, intensity of illumination gradient For 0,20,50,100,150,200,400,600,800,1 000,1200,1500,1800,2000,2200,2500 μm of ol m- 2s-1, Net Photosynthetic Rate corresponding to different illumination intensity is automatically recorded every 3min as system, it is quasi- to carry out photoresponse curve It closes.According to coefficient of determination R2It is found that the fitting effect of quadratic curve equation is preferable.Therefore, under artificial light source, with conic section Equation is fitted the photoresponse curve of Phalaris grass surface layer blade.Phalaris grass surface layer blade is in 8:00,10:00,12: 00, photoresponse curve corresponding to 14:00,16:00 is respectively y=-5 × 10-6x2+ 0.0150x+1.5636 (Fig. 1), y=-4 × 10-6x2+ 0.0147x+1.3829 (Fig. 2), y=-4 × 10-6x2+ 0.0136x+1.2515 (Fig. 3), y=-4 × 10-6x2+ 0.0124x+1.2094 (Fig. 4), y=-6 × 10-6x2+ 0.0176x+1.2854 (Fig. 5).
(2) Phalaris grass is measured respectively in 8:00,10:00,12:00,14:00,16:00 under natural lighting using biradial instrument Intensity of illumination under surface layer blade and the unobstructed environment of surrounding.It the results are shown in Table 1.
Intensity of illumination under 1 natural light of table under different time sections Phalaris grass surface layer blade and the unobstructed environment of surrounding
(3) photoresponse curve according to the Phalaris blade of grass piece being fitted in step (1), when calculating each under natural light in step (2) Between intensity of illumination under section Phalaris grass surface layer blade and the unobstructed environment of surrounding Net Photosynthetic Rate corresponding on photoresponse curve. It the results are shown in Table 2.
Intensity of illumination under 2 natural light of table under different periods Phalaris grass surface layer blade and the unobstructed environment of surrounding is in photoresponse song Corresponding Net Photosynthetic Rate on line
(4) illumination in step (3) under natural light under each period Phalaris grass surface layer blade and the unobstructed environment of surrounding is calculated Ratio of the intensity in the corresponding Net Photosynthetic Rate of photoresponse curve.As a result be respectively 71.61%, 73.41%, 76.29%, 78.31%, 77.43%.
Embodiment 2:
On May 5th, 2015, fair weather select reed Phalaris grass group, and sample area is 1m × 1m, and reed height is 2.0 Rice or so, density are 45 plants/m2, Phalaris natural plant height degree is 1.5m or so.Follow these steps to determine that Phalaris grass light is mended in reed Phalaris grass group Repay the method utilized.
(1) the portable photosynthetic instrument of LI-6400 of U.S. LI-COR company production, the following third piece expansion in measurement top are utilized Leaf central part.Utilize the artificial light source of instrument configuration, time 8:00,10:00,12:00,14:00,16:00, intensity of illumination gradient For 0,20,50,100,150,200,400,600,800,1 000,1200,1500,1800,2000,2200,2500 μm of ol m- 2s-1, Net Photosynthetic Rate corresponding to different illumination intensity is automatically recorded every 3min as system, it is quasi- to carry out photoresponse curve It closes.According to coefficient of determination R2It is found that the fitting effect of quadratic curve equation is preferable.Therefore, under artificial light source, with conic section Equation is fitted the photoresponse curve of Phalaris grass surface layer blade.Phalaris grass surface layer blade is in 8:00,10:00,12: 00, photoresponse curve corresponding to 14:00,16:00 is respectively y=-5 × 10-6x2+ 0.0150x+1.5636 (Fig. 1), y=-4 × 10-6x2+ 0.0147x+1.3829 (Fig. 2), y=-4 × 10-6x2+ 0.0136x+1.2515 (Fig. 3), y=-4 × 10-6x2+ 0.0124x+1.2094 (Fig. 4), y=-6 × 10-6x2+ 0.0176x+1.2854 (Fig. 5).
(2) Phalaris grass is measured respectively in 8:00,10:00,12:00,14:00,16:00 under natural lighting using biradial instrument Intensity of illumination under surface layer blade and the unobstructed environment of surrounding.It the results are shown in Table 3.
Intensity of illumination under 3 natural light of table under different time sections Phalaris grass surface layer blade and the unobstructed environment of surrounding
(3) photoresponse curve according to the Phalaris blade of grass piece being fitted in step (1), when calculating each under natural light in step (2) Between intensity of illumination under section Phalaris grass surface layer blade and the unobstructed environment of surrounding Net Photosynthetic Rate corresponding on photoresponse curve. It the results are shown in Table 4.
Intensity of illumination under 4 natural light of table under different time sections Phalaris grass surface layer blade and the unobstructed environment of surrounding is in photoresponse Corresponding Net Photosynthetic Rate on curve
(4) light in step (3) under natural light under each period Phalaris grass surface layer blade and the unobstructed environment of surrounding is calculated
According to intensity the corresponding Net Photosynthetic Rate of photoresponse curve ratio.As a result be respectively 35.81%, 36.05%, 39.98%, 35.79%, 33.26%.
Implementation result
It can be calculated by table 2, when reed density is 25 plants/m2When, the mean compensation algorithm of Phalaris grass net photosynthetic rate utilizes Rate is 75.44%, and table 4 is shown, when density is 45 plants/m2When, the mean compensation algorithm utilization rate of the Net Photosynthetic Rate of Phalaris blade of grass piece It is 36.18%.It follows that density is 25 plants/m2Reed canopy on Phalaris grass Net Photosynthetic Rate influence it is smaller, illumination can be tieed up The normal growth of Phalaris grass is held, and density is 45 plants/m2Reed canopy on Phalaris grass Net Photosynthetic Rate influence it is very big, illumination can not expire The normal growth of sufficient Phalaris grass, this is consistent with Phalaris grass growing way on the spot.In addition, Phalaris grass and reed Growth trends are shown (Fig. 6), two species During coexisting, the time that Phalaris grass is substantially less than reed is relatively short, and Phalaris grass is in seed grouting and latter stage of ripening, this is for root For clone plant Phalaris grass based on stem breeding, influence of the decline to a certain degree to its history of life of later period photosynthetic rate it is inevitable compared with It is small.Therefore, when reed density is 25 plants/m2When controlling or further increasing, this light compensation of Phalaris grass utilizes still can be certain Ensure that two species are metastable in degree and pattern coexists.Therefore this technology method can pass through the density of reed in control artificial swamp It is utilized to realize that the light of Phalaris grass compensates, to realize that the two stabilization coexists, it is not only competing to announcement wetland Phalaris grass-reed metapopulation It strives the support mechanism coexisted to be of great significance, while to other determining light with plant under Characteristics of Vertical Distribution group canopy Compensation is utilized and is also of great significance.

Claims (1)

1. Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group, it is characterised in that carry out as steps described below:
(1) density for selecting peak of growing season in reed Phalaris grass group is 25-45 plants/m2Reed canopy under Phalaris grass, it is main in one day In the light application time wanted, i.e. 8:00-16:00;Phalaris blade of grass piece photoresponse curve is carried out using portable photosynthetic instrument to be fitted, wherein Measurement light when measurement is set by endoprosthetic's light source that instrument carries, and sets intensity of illumination range as 0-2500 μm of ol m-2 s-1, system automatically records the Net Photosynthetic Rate of Phalaris blade of grass piece every 3min, and all is used to complete the photoresponse curve fitting of blade;
(2) density for selecting peak of growing season in reed Phalaris grass group is 25-45 plants/m2Reed canopy under Phalaris grass, utilize biradial Different time points Phalaris grass surface layer blade and surrounding unobstructed environment of the instrument under natural lighting, in determination step (1) the mentioned period Under intensity of illumination;
(3) photoresponse curve according to the Phalaris blade of grass piece being fitted in step (1) calculates in step (2) each time point under natural light Intensity of illumination under Phalaris grass surface layer blade and the unobstructed environment of surrounding Net Photosynthetic Rate corresponding on photoresponse curve;
(4) it calculates in step (3) under natural light under each time point Phalaris grass surface layer net photosynthetic rate and the unobstructed environment of surrounding The ratio of Net Photosynthetic Rate is spent, to estimate the light compensation utilization rate of Phalaris grass under reed canopy, and then determines that reed Phalaris grass is total Deposit the Suitable Density of reed in group.
CN201610128769.0A 2016-03-07 2016-03-07 Phalaris grass light compensates the determination method utilized in a kind of reed Phalaris grass group Expired - Fee Related CN105766284B (en)

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