CN101632473A - Apple probiotic fermented drink and production method - Google Patents

Apple probiotic fermented drink and production method Download PDF

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Publication number
CN101632473A
CN101632473A CN200910074472A CN200910074472A CN101632473A CN 101632473 A CN101632473 A CN 101632473A CN 200910074472 A CN200910074472 A CN 200910074472A CN 200910074472 A CN200910074472 A CN 200910074472A CN 101632473 A CN101632473 A CN 101632473A
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apple
probiotic
fermented drink
bifidobacterium
probio
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CN200910074472A
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赵敏
李爱梅
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Individual
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Abstract

An apple probiotic fermented drink is characterized by taking apple juice as a culture medium, fermenting and culturing such probiotics as bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and lactobacillus under certain temperature and time, then inactivating the fermented probiotic liquid and adding appropriate amount of white sugar to produce the drink. The residues produced in the process of producing the apple probiotic fermented drink can be used for producing apple probiotic jams. The invention greatly expands the application range of the culture medium materials of the probiotics such as bifidobacterium, provides a novel probiotic inactivated fermented drink for the consumers, enjoys rich raw material sources, low cost, stable production process, small investment on production equipment, simple operation and easy popularization and, has good market application prospect.

Description

A kind of apple probiotic fermented drink and preparation method
Technical field
The present invention relates to a kind of probiotic beverage of making by biofermentation, particularly relate to a kind of apple probiotic fermented drink and preparation method thereof.
Background technology
Fruit drink is liked by the consumer, has the supping of abundant nutrition.Yet at present the fruit drink of supply the market still is all not ideal enough on nutrition, the local flavor from mouthfeel, does not particularly have the delicate flavour of fresh fruit, and taste is more puckery.The use of additives such as artificial color and seasoning essence, thickener, anticorrisive agent makes fruit drink exist many unhealthful unsafe factors in addition.Therefore, existing fruit drink process technology has greatly limited the nutrition of fruit drink, the deep level development of functions such as natural color and utilization.
The drinks starting of the microorganism that utilization has a specific function by the fermentation brew soon, lactic acid drink and the acetic acid beverage that rises in the world is verified at present, the status of fermented beverage can not be ignored in the market of health drink from now on.The non-alcoholic fermented beverage also very lacks on China's beverage market, and therefore, exploitation trophism fermented beverage has great potential.
Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici etc. are the probios that is of value to health, generally be applied to treating disease of digestive system clinically, particularly helicobacter pylori is had and kill and inhibitory action, probio class good for health by clinical trial certificate, as can to strengthen human immunity.At present, having on the market with fresh cow milk, sheep breast is that bifid probio dairy produce or the medicine preparation that the culture medium fermentation is made occurs.But the bifid probiotic composition that occurs with sour milk and medicine viable bacteria goods is subjected to preserving the restriction with the bacterial activity cycle, and the use of probio has significant limitation.Particularly having greatly among the yellow, individuality has allergic reaction to ox, sheep lactoprotein, simple with the culture medium of cow's milk as probiotics fermention dairy products such as Bifidobacterium and medicine, greatly limited the market scope of application and the clinical application range of probios such as Bifidobacterium.
Summary of the invention
The purpose of this invention is to provide and a kind ofly probios such as Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici are seeded in apple squeeze the juice on the culture medium of making apple probiotic fermented drink that fermentation is made and preparation method thereof.
Apple probiotic fermented drink provided by the invention is to squeeze the juice with apple to make culture medium, the live vaccine of probios such as inoculated and cultured Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici, to finish the probio liquid deactivation of fermentation again and make beverage, and obtain apple probiotic material slag byproduct.
The preparation method of apple probiotic fermented drink of the present invention is as follows:
(1) preparation of probio liquid: will press into fruit juice in the stoning apple input juice extractor, put into pottery or stainless steel round after the sterilization, add the drinking water of 1~2 times of raw material weight and the probiotics viable bacteria seedling of raw material weight 0.3% under the continuous stirring, comprise 〉=1 * 10 7The Bifidobacterium of cfu/g, 〉=1 * 10 6The lactobacillus bulgaricus of cfu/g, 〉=1 * 10 6The streptococcus thermophilus of cfu/g and 〉=1 * 10 6The Bacillus acidi lactici of cfu/g, the control temperature is crossed 180~200 mesh sieves at 37~39 ℃ of fermentation 12~24h, obtains the apple probiotic virgin pulp liquid, and apple probiotic material slag is stocked usefulness in addition;
(2) making of probiotic beverage: the apple probiotic virgin pulp liquid is moved in the stainless steel boiling vessel, add the sugar of probio virgin pulp liquid weight 15~20%, 15~20% drinking water, boil 15~20min deactivation under the normal pressure, can, seal, sterilize and make product.
Probiotics viable bacteria seedlings such as natural Bifidobacterium of the present invention, Bulgarian lactic acid bacterium, streptococcus thermophilus, Bacillus acidi lactici are to extract, separate the solid, powdery live vaccine of making from fermentation cattle and sheep dairy products, can make by oneself, also can buy the commodity viable bacteria medicine that professional production producer provides, its proterties is a pressed powder.
The present invention also can produce apple probiotic material slag in the production process of producing apple probiotic fermented drink, this byproduct can further be processed into apple probiotic jam, and its preparation method is as follows:
To filter that last apple probiotic material slag moves in the stainless steel boiling vessel behind the apple probiotic virgin pulp liquid, add the sugar of pan feeding slag weight 20~25%, be brewed into the apple probiotic jam of water content 35~40%, and can, seal, sterilize and make product.
The present invention has the following advantages:
(1) squeezes the juice as the culture medium of probios such as Bifidobacterium with apple, greatly widened the range of application of probiotic bacteria culture medium selections such as Bifidobacterium;
(2) apple probiotic fermented drink of producing with the zymotic fluid of probios such as apple cultivation Bifidobacterium Bifidum has delicious taste and good health care function, for the consumer provides a kind of new type functional fruit fermented beverage;
(3) when producing apple probiotic fermented drink, utilize apple probiotic material slag can also be processed into apple probiotic jam, the apple culture medium raw material is fully utilized, thereby has improved utilization ratio of raw materials and added value;
(4) raw material sources of the present invention are abundant, and are with low cost, stable preparation process, and investment of production equipment is less, and is simple to operate, easily promotes, and the market competitiveness is strong.
The specific embodiment
Embodiment 1
With peeling, stoning, go fruit, Hua Di, the apple 100kg that cleans up drops in the large-scale fruit juice extractor and presses into fruit juice, moves in the ceramic fermentation reaction jar, under agitation adds drinking water 150kg and probio mixed live seedling 0.3kg, wherein, Bifidobacterium 〉=1 * 10 7Cfu/g, lactobacillus bulgaricus 〉=1 * 10 6Cfu/g, streptococcus thermophilus 〉=1 * 10 6Cfu/g, Bacillus acidi lactici 〉=1 * 10 6Cfu/g.Temperature is controlled at 37 ℃ of fermentation 24h, filters by 180 order stainless steel sifts, obtains the apple benefit and gives birth to virgin pulp liquid 180kg.The apple probiotic virgin pulp liquid is placed in the stainless steel boiling vessel, with drinking water 30kg, edible white granulated sugar 30kg, under normal pressure, boil the 20min deactivation, being filled into tinplate, aluminium alloy beverage can, glass or polyethylene beverage bottle seals, through ultra high temperature short time sterilization, be cooled to normal temperature, be up to the standards, be finished product.
Embodiment 2
With peeling, stoning, go fruit, Hua Di, the apple 150kg that cleans up drops in the large-scale fruit juice extractor and presses into fruit juice, moves in the ceramic fermentation reaction jar, under agitation adds drinking water 200kg and probio mixed live seedling 0.45kg, wherein, Bifidobacterium 〉=1 * 10 7Cfu/g, lactobacillus bulgaricus 〉=1 * 10 6Cfu/g, streptococcus thermophilus 〉=1 * 10 6Cfu/g, Bacillus acidi lactici 〉=1 * 10 6Cfu/g.Temperature is controlled at 38 ℃ of fermentation 24h, filters by 180 order stainless steel sifts, obtains the apple benefit and gives birth to virgin pulp liquid 270kg.The apple probiotic virgin pulp liquid is placed in the stainless steel boiling vessel, with drinking water 50kg, edible white granulated sugar 45kg, under normal pressure, boil the 15min deactivation, being filled into tinplate, aluminium alloy beverage can, glass or polyethylene beverage bottle seals, through ultra high temperature short time sterilization, be cooled to normal temperature, be up to the standards, be finished product.
Embodiment 3
Getting the apple probiotic material slag 100kg that filters behind the apple probiotic liquid packs in the stainless steel boiling vessel, add the edible white granulated sugar of 25kg, be brewed into moisture 36% apple probiotic jam, send into filling pipeline, pack into and seal in the vial, after the ultra high temperature short time sterilization operation, be cooled to normal temperature, be up to the standards, be finished product.

Claims (4)

1, a kind of apple probiotic fermented drink, it is characterized in that squeezing the juice as the medium culture probio with apple, beverage is made in the probio liquid deactivation of finishing fermentation, and obtaining apple probiotic material slag byproduct, wherein said probio is natural Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici.
2, apple probiotic fermented drink according to claim 1 is characterized in that described natural Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus, Bacillus acidi lactici are to extract the solid, powdery live vaccine of making from fermentation cattle and sheep dairy products.
3, the preparation method of the described apple probiotic fermented drink of claim 1 is characterized in that comprising the steps:
(1) preparation of probio liquid: will press into fruit juice in the stoning apple input juice extractor, put into pottery or stainless steel round after the sterilization, add the drinking water of 1~2 times of raw material weight and the probiotics viable bacteria seedling of raw material weight 0.3% under the continuous stirring, comprise 〉=1 * 10 7The Bifidobacterium of cfu/g, 〉=1 * 10 6The lactobacillus bulgaricus of cfu/g, 〉=1 * 10 6The streptococcus thermophilus of cfu/g and 〉=1 * 10 6The Bacillus acidi lactici of cfu/g, the control temperature is crossed 180~200 mesh sieves at 37~39 ℃ of fermentation 12~24h, obtains the apple probiotic virgin pulp liquid, and apple probiotic material slag is stocked usefulness in addition;
(2) making of probiotic beverage: the apple probiotic virgin pulp liquid is moved in the stainless steel boiling vessel, add the sugar of probio virgin pulp liquid weight 15~20%, 15~20% drinking water, boil 15~20min deactivation under the normal pressure, can, seal, sterilize and make product.
4, utilize the method for the apple probiotic material slag byproduct production apple probiotic jam that obtains in the described apple probiotic fermented drink production of claim 1, it is characterized in that last apple probiotic material slag behind the filtration apple probiotic virgin pulp liquid is moved in the stainless steel boiling vessel, the sugar that adds pan feeding slag weight 20~25%, be brewed into the apple probiotic jam of water content 35~40%, can, seal, sterilize and make product.
CN200910074472A 2009-05-25 2009-05-25 Apple probiotic fermented drink and production method Pending CN101632473A (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008055A (en) * 2010-12-21 2011-04-13 南昌大学 Fruit and vegetable jam and preparation method thereof
CN102028135A (en) * 2010-12-21 2011-04-27 南昌大学 Pawpaw sauce and preparation method thereof
CN102599589A (en) * 2012-03-22 2012-07-25 谭望桥 Method for preparing Noni fruit juice by fermentation method
CN103230076A (en) * 2013-04-02 2013-08-07 华南理工大学 Carrot and apple composite fruit and vegetable lactic acid fermented beverage and preparation method thereof
CN104223258A (en) * 2014-09-04 2014-12-24 佛山市新战略知识产权文化有限公司 Compound probiotic fermented fruit juice and preparation method thereof
CN104872771A (en) * 2015-06-10 2015-09-02 东北农业大学 Fruit and vegetable type beverage prepared through compound lactobacillus fermentation and preparing method thereof
CN105495237A (en) * 2015-12-17 2016-04-20 安徽砀山海升果业有限责任公司 Preparing method for fermented apple juice beverage
CN106261424A (en) * 2016-10-10 2017-01-04 河南酵益生物科技有限公司 A kind of Vegetable Drink Fermented containing active probiotic and preparation method thereof
CN107319236A (en) * 2017-08-18 2017-11-07 河南酵益生物科技有限公司 A kind of fermented beverage formula using apple as raw material and preparation method thereof
CN107668455A (en) * 2017-11-21 2018-02-09 咀香园健康食品(中山)有限公司 A kind of probiotics fermention passiflora edulis drink and preparation method thereof
CN109480151A (en) * 2018-10-26 2019-03-19 沈阳农业大学 A kind of apple probiotic ferment particulate beverage and preparation method thereof
CN111418746A (en) * 2020-04-07 2020-07-17 中国农业大学 Pumpkin and apple compound fermented beverage and preparation method and application thereof

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008055A (en) * 2010-12-21 2011-04-13 南昌大学 Fruit and vegetable jam and preparation method thereof
CN102028135A (en) * 2010-12-21 2011-04-27 南昌大学 Pawpaw sauce and preparation method thereof
CN102008055B (en) * 2010-12-21 2013-07-31 南昌大学 Fruit and vegetable jam and preparation method thereof
CN102599589A (en) * 2012-03-22 2012-07-25 谭望桥 Method for preparing Noni fruit juice by fermentation method
CN103230076A (en) * 2013-04-02 2013-08-07 华南理工大学 Carrot and apple composite fruit and vegetable lactic acid fermented beverage and preparation method thereof
CN104223258A (en) * 2014-09-04 2014-12-24 佛山市新战略知识产权文化有限公司 Compound probiotic fermented fruit juice and preparation method thereof
CN104872771A (en) * 2015-06-10 2015-09-02 东北农业大学 Fruit and vegetable type beverage prepared through compound lactobacillus fermentation and preparing method thereof
CN105495237A (en) * 2015-12-17 2016-04-20 安徽砀山海升果业有限责任公司 Preparing method for fermented apple juice beverage
CN106261424A (en) * 2016-10-10 2017-01-04 河南酵益生物科技有限公司 A kind of Vegetable Drink Fermented containing active probiotic and preparation method thereof
CN107319236A (en) * 2017-08-18 2017-11-07 河南酵益生物科技有限公司 A kind of fermented beverage formula using apple as raw material and preparation method thereof
CN107668455A (en) * 2017-11-21 2018-02-09 咀香园健康食品(中山)有限公司 A kind of probiotics fermention passiflora edulis drink and preparation method thereof
CN109480151A (en) * 2018-10-26 2019-03-19 沈阳农业大学 A kind of apple probiotic ferment particulate beverage and preparation method thereof
CN111418746A (en) * 2020-04-07 2020-07-17 中国农业大学 Pumpkin and apple compound fermented beverage and preparation method and application thereof

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Application publication date: 20100127