CN101637290A - Grape probiotic fermentation beverage and making method - Google Patents
Grape probiotic fermentation beverage and making method Download PDFInfo
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- CN101637290A CN101637290A CN200910074466A CN200910074466A CN101637290A CN 101637290 A CN101637290 A CN 101637290A CN 200910074466 A CN200910074466 A CN 200910074466A CN 200910074466 A CN200910074466 A CN 200910074466A CN 101637290 A CN101637290 A CN 101637290A
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Abstract
The invention relates to a grape probiotic fermentation beverage. Squeeze juice of grape is used as a culture medium; fermentation is carried out under specific temperature and for specific time to culture probiotics, such as bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus, lactic acid bacillus and the like; then, probiotic liquid after fermentation is inactivated, and then aproper amount of white granulated sugar is added to make a beverage. Slag byproducts generated in the production of the grape probiotic fermentation beverage can be also used for producing grape probiotic jam. The invention greatly broadens the application range of probiotic culture medium selection of materials, such as bifidobacterium and the like, provides a novel probiotic inactivation fermentation beverage, and has the advantages of rich material source, low cost, stable preparation process, little production equipment investment, simple operation, easy popularization and favorable market application prospect.
Description
Technical field
The present invention relates to a kind of probiotic beverage of making by biofermentation, particularly relate to a kind of grape probiotic fermentation beverage and preparation method thereof.
Background technology
Fruit drink is liked by the consumer, has the supping of abundant nutrition.Yet at present the fruit drink of supply the market still is all not ideal enough on nutrition, the local flavor from mouthfeel, does not particularly have the delicate flavour of fresh fruit, and taste is more puckery.The use of additives such as artificial color and seasoning essence, thickener, anticorrisive agent makes fruit drink exist many unhealthful unsafe factors in addition.Therefore, existing fruit drink process technology has greatly limited the nutrition of fruit drink, the deep level development of functions such as natural color and utilization.
The drinks starting of the microorganism that utilization has a specific function by the fermentation brew soon, lactic acid drink and the acetic acid beverage that rises in the world is verified at present, the status of fermented beverage can not be ignored in the market of health drink from now on.The non-alcoholic fermented beverage also very lacks on China's beverage market, and therefore, exploitation trophism fermented beverage has great potential.
Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici etc. are the probios that is of value to health, generally be applied to treating disease of digestive system clinically, particularly helicobacter pylori is had and kill and inhibitory action, probio class good for health by clinical trial certificate, as can to strengthen human immunity.At present, having on the market with fresh cow milk, sheep breast is that bifid probio dairy produce or the medicine preparation that the culture medium fermentation is made occurs.But the bifid probiotic composition that occurs with sour milk and medicine viable bacteria goods is subjected to preserving the restriction with the bacterial activity cycle, and the use of probio has significant limitation.Particularly having greatly among the yellow, individuality has allergic reaction to ox, sheep lactoprotein, simple with the culture medium of cow's milk as probiotics fermention dairy products such as Bifidobacterium and medicine, greatly limited the market scope of application and the clinical application range of probios such as Bifidobacterium.
Summary of the invention
The purpose of this invention is to provide and a kind ofly probios such as Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici are seeded on the culture medium that grape extraction juice makes grape probiotic fermentation beverage that fermentation is made and preparation method thereof.
Grape probiotic fermentation beverage provided by the invention is to make culture medium with grape extraction juice, the live vaccine of probios such as inoculated and cultured Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici, to finish the probio liquid deactivation of fermentation again and make beverage, and obtain grape probio material slag byproduct.
The preparation method of grape probiotic fermentation beverage of the present invention is as follows:
(1) preparation of probio liquid: will press into fruit juice in the stoning grape input juice extractor, put into pottery or stainless steel round after the sterilization, add the drinking water of 1～2 times of raw material weight and the probiotics viable bacteria seedling of raw material weight 0.3% under the continuous stirring, comprise 〉=1 * 10
7The Bifidobacterium of cfu/g, 〉=1 * 10
6The lactobacillus bulgaricus of cfu/g, 〉=1 * 10
6The streptococcus thermophilus of cfu/g and 〉=1 * 10
6The Bacillus acidi lactici of cfu/g, the control temperature is crossed 180～200 mesh sieves at 25～30 ℃ of fermentation 24～28h, obtains grape probio virgin pulp liquid, and grape probio material slag is stocked usefulness in addition;
(2) making of probiotic beverage: grape probio virgin pulp liquid is moved in the stainless steel boiling vessel, add the sugar of probio virgin pulp liquid weight 15～20%, 15～20% drinking water, boil 15～20min deactivation under the normal pressure, can, seal, sterilize and make product.
Probiotics viable bacteria seedlings such as natural Bifidobacterium of the present invention, Bulgarian lactic acid bacterium, streptococcus thermophilus, Bacillus acidi lactici are to extract, separate the solid, powdery live vaccine of making from fermentation cattle and sheep dairy products, can make by oneself, also can buy the commodity viable bacteria medicine that professional production producer provides, its proterties is a pressed powder.
The present invention also can produce grape probio material slag in the production process of producing grape probiotic fermentation beverage, this byproduct can further be processed into grape probio jam, and its preparation method is as follows:
To filter that last grape probio material slag moves in the stainless steel boiling vessel behind the grape probio virgin pulp liquid, add the sugar of pan feeding slag weight 20～25%, be brewed into the grape probio jam of water content 32～35%, and can, seal, sterilize and make product.
The present invention has the following advantages:
(1), greatly widened the range of application of probiotic bacteria culture medium selections such as Bifidobacterium with the culture medium of grape extraction juice as probios such as Bifidobacteriums;
(2) grape probiotic fermentation beverage of producing with the zymotic fluid of probios such as grape cultivation Bifidobacterium Bifidum has delicious taste and good health care function, for the consumer provides a kind of new type functional fruit fermented beverage;
(3) when producing grape probiotic fermentation beverage, utilize grape probio material slag can also be processed into grape probio jam, the grape culture medium raw material is fully utilized, thereby has improved utilization ratio of raw materials and added value;
(4) raw material sources of the present invention are abundant, and are with low cost, stable preparation process, and investment of production equipment is less, and is simple to operate, easily promotes, and the market competitiveness is strong.
The specific embodiment
Embodiment 1
With clean up, the fresh grape 100kg of stoning drops in the large-scale fruit juice extractor and presses into fruit juice, moves in the ceramic fermentation reaction jar, under agitation adds drinking water 150kg and probio mixed live seedling 0.3kg, wherein, Bifidobacterium 〉=1 * 10
7Cfu/g, lactobacillus bulgaricus 〉=1 * 10
6Cfu/g, streptococcus thermophilus 〉=1 * 10
6Cfu/g, Bacillus acidi lactici 〉=1 * 10
6Cfu/g.Temperature is controlled at 28 ℃, and fermentation 24h filters by 180 order stainless steel sifts, obtains the grape benefit and gives birth to virgin pulp liquid 220kg.Grape probio virgin pulp liquid is placed in the stainless steel boiling vessel, with drinking water 40kg, edible white granulated sugar 40kg, under normal pressure, boil the 20min deactivation, being filled into tinplate, aluminium alloy beverage can, glass or polyethylene beverage bottle seals, through ultra high temperature short time sterilization, be cooled to normal temperature, be up to the standards, be finished product.
Embodiment 2
With clean up, the fresh grape 150kg of stoning drops in the large-scale fruit juice extractor and presses into fruit juice, moves in the stainless steel fermentation reaction jar, stir to add drinking water 200kg and probio mixed live seedling 0.45kg down, wherein, Bifidobacterium 〉=1 * 10
7Cfu/g, lactobacillus bulgaricus 〉=1 * 10
6Cfu/g, streptococcus thermophilus 〉=1 * 10
6Cfu/g, Bacillus acidi lactici 〉=1 * 10
6Cfu/g.Temperature is controlled at 26 ℃, and fermentation 26h filters by 200 order stainless steel sifts, obtains the grape benefit and gives birth to virgin pulp liquid 300kg.Grape probio virgin pulp liquid is placed in the stainless steel boiling vessel, with drinking water 45kg, edible white granulated sugar 60kg, under normal pressure, boil the 15min deactivation, being filled into tinplate, aluminium alloy beverage can, glass or polyethylene beverage bottle seals, through ultra high temperature short time sterilization, be cooled to normal temperature, be up to the standards, be finished product.
Embodiment 3
Getting the grape probio material slag 100kg that filters behind the grape probio liquid packs in the stainless steel boiling vessel, add the edible white granulated sugar of 25kg, be brewed into moisture 32% grape probio jam, send into filling pipeline, pack into and seal in the vial, after the ultra high temperature short time sterilization operation, be cooled to normal temperature, be up to the standards, be finished product.
Claims (4)
1, a kind of grape probiotic fermentation beverage, it is characterized in that with grape extraction juice as the medium culture probio, beverage is made in the probio liquid deactivation of finishing fermentation, and obtaining grape probio material slag byproduct, wherein said probio is natural Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and Bacillus acidi lactici.
2, grape probiotic fermentation beverage according to claim 1 is characterized in that described natural Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus, Bacillus acidi lactici are to extract the solid, powdery live vaccine of making from fermentation cattle and sheep dairy products.
3, the preparation method of the described grape probiotic fermentation beverage of claim 1 is characterized in that comprising the steps:
(1) preparation of probio liquid: will press into fruit juice in the stoning grape input juice extractor, put into pottery or stainless steel round after the sterilization, add the drinking water of 1～2 times of raw material weight and the probiotics viable bacteria seedling of raw material weight 0.3% under the continuous stirring, comprise 〉=1 * 10
7The Bifidobacterium of cfu/g, 〉=1 * 10
6The lactobacillus bulgaricus of cfu/g, 〉=1 * 10
6The streptococcus thermophilus of cfu/g and 〉=1 * 10
6The Bacillus acidi lactici of cfu/g, the control temperature is crossed 180～200 mesh sieves at 25～30 ℃ of fermentation 24～28h, obtains grape probio virgin pulp liquid, and grape probio material slag is stocked usefulness in addition;
(2) making of probiotic beverage: grape probio virgin pulp liquid is moved in the stainless steel boiling vessel, add the sugar of probio virgin pulp liquid weight 15～20%, 15～20% drinking water, boil 15～20min deactivation under the normal pressure, can, seal, sterilize and make product.
4, utilize the method for the grape probio material slag byproduct production grape probio jam that obtains in the described grape probiotic fermentation beverage production of claim 1, it is characterized in that last grape probio material slag behind the filtration grape probio virgin pulp liquid is moved in the stainless steel boiling vessel, the sugar that adds pan feeding slag weight 20～25%, be brewed into the grape probio jam of water content 32～35%, can, seal, sterilize and make product.
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| CN200910074466A CN101637290A (en) | 2009-05-25 | 2009-05-25 | Grape probiotic fermentation beverage and making method |
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| CN200910074466A CN101637290A (en) | 2009-05-25 | 2009-05-25 | Grape probiotic fermentation beverage and making method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102356913A (en) * | 2011-08-31 | 2012-02-22 | 哈尔滨北极人商贸有限公司 | Preparation method of probiotics fermented blueberry pulp powder |
| CN103976437A (en) * | 2014-04-29 | 2014-08-13 | 河南科技大学 | Preparing method of plant fermented beverage with antioxidant activity |
| CN105286006A (en) * | 2015-09-09 | 2016-02-03 | 周学义 | Pure grape essence ferment and production method thereof |
| CN106261424A (en) * | 2016-10-10 | 2017-01-04 | 河南酵益生物科技有限公司 | A kind of Vegetable Drink Fermented containing active probiotic and preparation method thereof |
| CN106282066A (en) * | 2016-08-24 | 2017-01-04 | 庐江县白山镇金沈葡萄种植园 | A kind of mixture cultural method of probiotic bacteria and organic acid and application thereof |
| CN109953321A (en) * | 2017-12-25 | 2019-07-02 | 海南合生源生物科技有限公司 | The preparation method of grape ferment |
| CN114145403A (en) * | 2021-12-10 | 2022-03-08 | 山西娇禾生物科技有限公司 | Grape probiotic health-care stock solution capable of strengthening spleen and stomach, benefiting intestines and expelling toxin and preparation method thereof |
-
2009
- 2009-05-25 CN CN200910074466A patent/CN101637290A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102356913A (en) * | 2011-08-31 | 2012-02-22 | 哈尔滨北极人商贸有限公司 | Preparation method of probiotics fermented blueberry pulp powder |
| CN103976437A (en) * | 2014-04-29 | 2014-08-13 | 河南科技大学 | Preparing method of plant fermented beverage with antioxidant activity |
| CN105286006A (en) * | 2015-09-09 | 2016-02-03 | 周学义 | Pure grape essence ferment and production method thereof |
| CN106282066A (en) * | 2016-08-24 | 2017-01-04 | 庐江县白山镇金沈葡萄种植园 | A kind of mixture cultural method of probiotic bacteria and organic acid and application thereof |
| CN106261424A (en) * | 2016-10-10 | 2017-01-04 | 河南酵益生物科技有限公司 | A kind of Vegetable Drink Fermented containing active probiotic and preparation method thereof |
| CN109953321A (en) * | 2017-12-25 | 2019-07-02 | 海南合生源生物科技有限公司 | The preparation method of grape ferment |
| CN114145403A (en) * | 2021-12-10 | 2022-03-08 | 山西娇禾生物科技有限公司 | Grape probiotic health-care stock solution capable of strengthening spleen and stomach, benefiting intestines and expelling toxin and preparation method thereof |
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Application publication date: 20100203 |