CN101595836B - Method for preservation of pure state of hybrid plants by chemical emasculation - Google Patents
Method for preservation of pure state of hybrid plants by chemical emasculation Download PDFInfo
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- CN101595836B CN101595836B CN2009100231626A CN200910023162A CN101595836B CN 101595836 B CN101595836 B CN 101595836B CN 2009100231626 A CN2009100231626 A CN 2009100231626A CN 200910023162 A CN200910023162 A CN 200910023162A CN 101595836 B CN101595836 B CN 101595836B
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- imazethapyr
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Abstract
The present invention discloses a method for preservation of pure state of hybrid plants by chemical emasculation. In the method, a breed sensitive to acetolactate synthase inhibitory type herbicide is selected as female parent and a breed resistant to acetolactate synthase inhibitory type herbicide is selected as male parent, acetolactate synthase inhibitory type herbicide with activity of chemical hybridizing agent is used for processing the female parent to obtain male sterility, the male parent pollinates the female parent, the seeds of the female parent line are harvested to be cross breeds, the obtained F1 generation cross breed has herbicide resistance, after the F1 generation cross breed is planted, and acetolactate synthase inhibitory type herbicide seedling stage processing is used for removing uncrossed female parent seedling, so as to remain real cross breed. The method utilizes herbicide for preservation of pure state while realizing weeding. The method for preservation of pure state of hybrid plants by chemical emasculation provided by the present invention has more advantages than common breed in the aspect of adapted breed simplified culture requirement.
Description
Technical field
The invention belongs to crop hybrid superiority and utilize the field, relate to production, the kind method for planting of chemical emasculation crossbreed, particularly a kind of state of hybrid plants by chemical emasculation is protected pure method.
Background technology
The intervarietal hybrid advantage of many crops various degrees such as cabbage type rape of gramineous plants wheat, Cruciferae.Chemical emasculation is one of feasible way that utilizes crop heterosis, by using chemical hybridizing agent (or claiming male gametocide) inducing plant male sterile, promotes cross pollination, obtains plant hybrid.
Chemical emasculation is one of main path of wheat heterosis utilization at present.The efficient chemical hybridizing agent GENESIS of Monsanto Company's development has started chemical hybridization wheat research climax.Domestic chemical hybridizing agent SQ-1, the BAU9403 of also having developed.But, limited the popularization of wheat hybrid because of hybrid purity is difficult to guarantee to wait some problems.
Recent study is found some key enzyme at the route of synthesis of plant branched-chain amino acid leucine, isoleucine, valine---the weed killer herbicide of acetolactate synthestase ALS (or acetohydroxy acid synthetase AHAS) has the chemical emasculation activity to sensitive plant such as rape, wheat etc.(Yu et al, Inducingmale sterility in Brassica napus L.by a sulfonylurea herbicide, tribenuron-methyl, Plant Breeding, 2006,125 (1): 61-64 such as Yu; Yu et al, Efficiencyof a novel gametocide amidosulfuron on rapeseed (Brassica napus) .PlantBreeding 2009, DOI:10.1111/j.1439-0523.2008.01594.x) and the Chinese patent (patent No.: ZL200610042886.1, Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology, Yu Chengyu, Hu Shengwu, a kind of compound is used to prepare the application of brassica plant chemical hybridizing agent), Chinese patent application, application number: 200810231884.6 (Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology, Yu Chengyu, Hu Shengwu, compound (RS)-5-ethyl-2-(4-isopropyl-4-methyl-5-oxo-2-imidazoline-2-yl) nicotinic acid and ammonium salt thereof are used for the application as Phytochemistry hybridization agent) wait the document priority to disclose some sulfonylureas, imidazolinone herbicide such as tribenuron-methyl (tribenuron-methyl), amidosulfuron (amidosulfuron), Imazethapyr (imazethapyr) is as the sensitive plant rape, the super-high-efficient chemical hybridizing agent of wheat etc., using dosage is 1/10～1/100 of a weed killer herbicide usage amount, and cost is very cheap.
Because the chemical emasculation production of hybrid seeds has very high specification requirement, its effect is subjected to weather, maternal growing way, the maternal uniformity, sprays the restriction of multiple factors such as period, dosage order of accuarcy, when the large tracts of land production of hybrid seeds, deviation can appear unavoidably, just can not guarantee to obtain the crossbreed of 100% purity, even the purity that connects more than 90% can not guarantee fully.How improving purity of hybrid is one of main difficult problem of chemical emasculation approach.The genetic marker that utilization is convenient to discern for example mark parents such as wax powder-free, floral leaf, etiolated seedling so that F
1For impurity elimination is feasible, but needs to pull out assorted seedling by hand, and labour intensity is bigger.Zoltan Barabas proposes, available in theory recessive auxotrophic mutation is as female parent, filial generation nutrition is defective not, and maternal self progeny can automatically dead (Zoltan Barabas.Utilization of auxotrophy inhybrid seed production for elimination of the disadvantages of gametocides.Euphytica when planting, 1993,70:75-76).But the feasibility of this scheme is not verified.
In view of the chemical weed control technology is very general, coming removal of impurities to protect pure in conjunction with chemical herbicide is a feasible way.For example, Chinese patent application, application number: 200610048242.3 (a kind of kind method for planting of millet cenospecies of anti weedicide), Chinese patent application, publication number: CN97109814.X (production method of hybrid rice seed), anti-herbicide gene is changed over to the male sterile line of crossbreed or recovers system, produce hybrid seed.Utilize weed killer herbicide to remove the pseudostationary plant then.But adopt weed killer herbicide that the pure bibliographical information of not seeing is protected in the impurity elimination of chemical emasculation crossbreed, particularly protect the pure research that also do not obtain for the impurity elimination of the crossbreed of acetolactate synthestase inhibition type chemical hybridizing agent preparation.Even the chemical emasculation crossbreed also can educate entirely because crossbreed purity is not high, generally can not cause the dispute of seed purity problem, purity for the chemical emasculation crossbreed improves at present, also do not give enough attention, this can cause varietal purity not high, and the volume increase advantage is difficult to bring into play fully.
Discover that plant is variant to the susceptibility of sulfonylurea, imidazolinone herbicide.Particularly induced mutations or transgenosis can obtain high anti-type.Can obtain herbicide resistant plants (Su Shaoquan by the trans-exogenous resistant gene, the initiative and the plantation of transgenosis antiweed rape, 2006,45 the 5th phases of volume), the gene that transforms is owing to be subjected to strong promoter to drive expression, generally all follow simple dominance Mendelian inheritance pattern, can be as a kind of important genetic screening mark.Du pont company has been developed the genetically engineered soybean (STS of sulfonylurea herbicides such as anti-chlorimuronethyl and thifensulfuron methyl
) and cotton (19-51acotton), thereby make the field weeding that is applied to soybean and cotton that above-mentioned weed killer herbicide kind can safety.
The Ala122 of encoding acetolactate synthase albumen in the arabidopsis, Pro197, Ala205, Trp574, the DNA base of Ser653 and Gly654 is replaced and can be produced Herbicid resistant sudden change (Wang Xiujun, Lang Zhihong, Dan Anshan, Huang Dafang, amino acid bio synthetic inhibitor class herbicide action mechanism and herbicide-resistant transgenic plant research progress, Chinese biological engineering magazine, 2008,20 (2): 110-116).Because the action target of weed killer herbicides such as sulfonylureas, imidazolone, triazolo pyrimidine class is identical, a lot of acetolactate synthase gene sudden changes present the cross tolerance to similar weed killer herbicide or different weed killer herbicides.Obtain acetolactate synthase gene sudden change (the Swanson EB of cabbage type rape by mutagenesis, MJ Herrgesell, MArnoldo, DW Sippell.Microspore mutagenesis and selection:Canola plants withfield tolerance to the imidazolinones.Theor.and Appl.Genet., 1989,78 (4): 525-530).Company of American Cyanamid Company obtains imidazolinone herbicide Imazethapyr etc. is had wheat (IMI-wheat), the rape (IMI-canola) of height resistance by artificial screening, by BASF Aktiengesellschaft with
For trade mark and realization large-scale commercial applicationsization, can spray Imazethapyr in the resistant variety field and carry out chemical weed control, common variety can not be used this weed killer herbicide because of susceptibility is too high.The CLEARFIELD resistant gene presents the dominant inheritance mode, in crossbreed, obtain performance (Zhang WQ Risk assessment of the transfer of imazethapyr herbicide tolerance fromClearfield rice to red rice (Oryza sativa), Euphytica, 2006,152 (1): 75-86).
Summary of the invention
The objective of the invention is to, provide a kind of chemical emasculation crossbreed impurity elimination to protect pure method, this method is particularly suitable for impurity eliminations such as chemical emasculation hybrid plant such as rape, wheat and protects pure.
In order to realize above-mentioned task, the present invention takes following technical solution:
Pure method is protected in a kind of chemical emasculation crossbreed impurity elimination, it is characterized in that, this method is when the preparing hybrid kind, select for use acetolactate synthestase inhibition type herbicide sensitive kind is made female parent, select for use acetolactate synthestase inhibition type Herbicid resistant kind as male parent, obtain male sterile with the acetolactate synthestase inhibition type herbicide treatment female parent with chemical hybridizing agent activity, male parent is given maternal pollination, the maternal row of results seed is crossbreed, the F of acquisition
1Has Herbicid resistant for crossbreed, F
1After the crossbreed plantation, handle the young plant of removing the female parent of wherein not hybridizing seedling stage with acetolactate synthestase inhibition type weed killer herbicide, keep true crossbreed.
In the acetolactate synthestase inhibition type weed killer herbicide, there are the compound that sulfonylurea is arranged, particularly tribenuron-methyl, amidosulfuron, pyrazosulfuron, the chlorine sulphur of chemical hybridizing agent activity grand to rape, perhaps imidazolone compound, for example Imazethapyr; Be suitable for the imidazolone compound that has of wheat, for example Imazethapyr.Plant as male parent should be with the resistance to acetolactate synthestase inhibition type weed killer herbicide by dominant gene control.
Because some sudden change has cross tolerance to different acetolactate synthestase inhibition type weed killer herbicides, so used chemical hybridizing agent can be identical with weed killer herbicide among these father and mother, also can be different.
The present invention and other are that the method main distinction that pure means are protected in impurity elimination is with the weed killer herbicide, ingenious utilized some acetolactate synthestase inhibition type weed killer herbicides can be simultaneously as the characteristics of chemical hybridizing agent, utilize female parent, the male parent of hybrid combination the difference of herbicide sensitive to be realized a compounds, two kinds of functions.Usage amount during as chemical hybridizing agent is 1/10～1/100 of a weed killer herbicide dosage, and because of male parent has resistance, spray need not be blocked the male parent row because of worrying poisoning during medicine, can be relieved whole seed farm is sprayed.The dominance resistant gene of male parent has guaranteed F
1In generation,, true hybrid had resistance, even there is maternal self progeny to sneak into, it is pure also to use the weed killer herbicide impurity elimination to protect, and has played the weeding purpose simultaneously.Gently simplify cultivation in adapted breed and have bigger advantage than common variety aspect requiring.
Embodiment
The present invention is described in further detail below in conjunction with embodiment that the inventor provides, the invention is not restricted to these embodiment.
Embodiment 1:
With common wheat is maternal, and Imazethapyr resistance wheat is as male parent, and this went father and mother than plantation at interval with 2: 8.When the female parent master is tillered young spike length degree 1cm～1.5cm, and the herbicide spraying Imazethapyr (spray is in right amount: 7g Imazethapyr/hectare), and to induce male sterile.Treat maternal heading, when clever shell is opened a business, manually catch up with powder, promptly shake male parent and pollinate, the maternal row of maturing stage results seed to female parent with stay cord or bamboo pole.
Plantation gained seed, (spray is in right amount: 70g Imazethapyr/hectare), pseudostationary and weeds are killed seedling spraying weed killer herbicide Imazethapyr, and true hybrid has resistance and survived.
Embodiment 2:
With cabbage type rape variety AC Elect is maternal, and Imazethapyr resistance rape is as male parent, and this went father and mother than plantation at interval with 1: 2.When the maximum bud length of female parent is 2.5cm～3cm, and the herbicide spraying amidosulfuron (amount of spraying: the 0.4mg amidosulfuron/L), to induce male sterile.When treating that female parent is bloomed, shake male parent and pollinate, the maternal row of maturing stage results seed to female parent.
Plantation gained seed, (spray is in right amount: 70g Imazethapyr/hectare), pseudostationary and weeds are killed seedling spraying weed killer herbicide Imazethapyr, and true hybrid has resistance and survived.
Claims (1)
1. a state of hybrid plants by chemical emasculation is protected pure method, it is characterized in that:
With common wheat is maternal, and Imazethapyr resistance wheat is as male parent, father and mother this with 2: 8 row than plantation at interval, when the female parent master is tillered young spike length degree 1cm～1.5cm, the herbicide spraying Imazethapyr, spray is in right amount: 7g Imazethapyr/hectare, to induce male sterile;
Treat maternal heading, when clever shell is opened a business, manually catch up with powder, promptly shake male parent and pollinate, the maternal row of maturing stage results seed to female parent with stay cord or bamboo pole;
Plantation gained seed, seedling spraying weed killer herbicide Imazethapyr, spray is in right amount: 70g Imazethapyr/hectare, pseudostationary and weeds are killed, and true hybrid has resistance and is survived;
Perhaps:
With cabbage type rape variety AC Elect is maternal, and Imazethapyr resistance rape is as male parent, and this went father and mother than plantation at interval with 1: 2;
When the maximum bud length of female parent is 2.5cm～3cm, the herbicide spraying amidosulfuron, the amount of spraying: 0.4mg amidosulfuron/L, to induce male sterile;
When treating that female parent is bloomed, shake male parent and pollinate, the maternal row of maturing stage results seed to female parent;
Plantation gained seed, seedling spraying weed killer herbicide Imazethapyr, spray is in right amount: 70g Imazethapyr/hectare, pseudostationary and weeds are killed, and true hybrid has resistance and is survived.
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| CN101595836B true CN101595836B (en) | 2011-11-09 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810134B (en) * | 2010-03-19 | 2012-01-18 | 西北农林科技大学 | Seed production method for producing two-line hybrid rape by using photoperiod-temperature sensitive genic male sterile rape in winter rape area |
| CN101810135B (en) * | 2010-03-19 | 2011-11-30 | 西北农林科技大学 | Seed production method for producing high-purity hybrid rape seeds by using double-pollination control system |
| CN103237441B (en) * | 2011-11-02 | 2014-12-03 | 未名兴旺系统作物设计前沿实验室（北京）有限公司 | Method for maintaining nuclear male sterility line of wheat cells |
| CN102763506B (en) * | 2012-06-25 | 2014-02-05 | 北京兴博雅生物技术有限公司 | Method for increasing purity of plant variety |
| CN106165645A (en) * | 2016-05-25 | 2016-11-30 | 中国农业科学院油料作物研究所 | Cross rape producing method for seed based on Herbicid resistant and application thereof |
| CN108935079B (en) * | 2018-08-15 | 2021-06-25 | 华中农业大学 | Simplified seed production method of rape hybrid F1 seed |
| CN109511547A (en) * | 2018-12-28 | 2019-03-26 | 陕西省杂交油菜研究中心 | A kind of non-transgenic matter infertility antiweed hybrid seeds technology |
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| CN1263701A (en) * | 1999-01-27 | 2000-08-23 | 黄大年 | Breeding method for hybridized rice |
| CN1135916C (en) * | 1999-03-29 | 2004-01-28 | 湖北省农业科学院农业现代化研究所 | Method for removing female parent selfed seedling from rice hybrid strain |
| CN1251597C (en) * | 2003-02-28 | 2006-04-19 | 陕西省杂交油菜研究中心 | Chemical crossbred agent composition, its using method and application |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1263701A (en) * | 1999-01-27 | 2000-08-23 | 黄大年 | Breeding method for hybridized rice |
| CN1135916C (en) * | 1999-03-29 | 2004-01-28 | 湖北省农业科学院农业现代化研究所 | Method for removing female parent selfed seedling from rice hybrid strain |
| CN1251597C (en) * | 2003-02-28 | 2006-04-19 | 陕西省杂交油菜研究中心 | Chemical crossbred agent composition, its using method and application |
Non-Patent Citations (1)
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