CN101590175B - Pharmaceutical preparation with function of regulating blood sugar level and production method thereof - Google Patents
Pharmaceutical preparation with function of regulating blood sugar level and production method thereof Download PDFInfo
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Abstract
The invention discloses a pharmaceutical preparation with function of regulating blood sugar level and a production method thereof, which are characterized by being an extract obtained from pseudo-ginseng, codonopsis pilosula, Szechuan lovage rhizome, the root of red-rooted salvia, rhizoma anemarrhenae, and mulberry leaf and prepared into solid preparation, wherein the parts by weight is as follows: 1-10 of pseudo-ginseng, 1-10 of codonopsis pilosula, 1-10 of Szechuan lovage rhizome, 1-5 of the root of red-rooted salvia, 1-5 of rhizoma anemarrhenae and 1-5 of mulberry leaf. Shown by pharmacologic experiment result, the granule preparation of the invention has better pharmacologic efficacy in regulating blood sugar level.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of pharmaceutical preparation and preparation method thereof with hypoglycemic activity.
Background technology
Raising along with China's living standards of the people; Great changes have taken place in people's dietary structure, and long-term irrational diet makes the long-term secretion of intravital insulin more; Cause the insulin target cell that the sensitivity of insulin is reduced gradually; Thereby produced hyperglycemia, the sickness rate of diabetes also improves thereupon, and diabetes are a kind of whole body chronic progressive diseases.Because the normal excreting insulin of islet cells, thereby insulin relatively or absolute deficiency, and target cell is to the reduction of insulin sensitivity, and cause sugar, albumen, fat and water, metabolic disturbance of electrolyte, and hepatic glycogen and muscle glycogen can not be synthesized.Clinical manifestation is that blood sugar increasing, the glucose in urine positive and carbohydrate tolerance reduce, and classical symptom is " three-many-one-little ", i.e. polydipsia, polyuria, polyphagia and lose weight; For a long time it, in succession with a series of complication; The traditional Chinese medical science claims that diabetes are diabetes.With China is example, brought up to by 0.5% of the past present about 3%, at present; In case the medicine of treatment diabetes biases toward mostly stimulates islets of langerhans to produce insulin, and the target cell of insulin does not obtain basic improvement to the sensitivity of insulin, so after drug withdrawal a period of time; Blood glucose is bounce-back once more often, simultaneously, because long-term prescription is to the stimulation of islets of langerhans; Make islets of langerhans be in the duty of long-term anxiety, and finally cause the tired out of islet function, cause the diabetes patient finally to have to help blood sugar lowering by external insulin; Can further cause the part or all of forfeiture of islet function like this, because blood glucose rebounds repeatedly, the viscosity of blood increased simultaneously; The microcirculation generation obstacle of blood, the final complication that aspects such as the heart, brain, kidney and cutaneous nerve still can take place.
Diabetes can betide any age; Along with the course of disease prolongs; Easy concurrent whole body nerve, blood capillary, macroangiopathy, and can cause the chronic progressive external pathological changes of histoorgans such as the heart, brain, kidney, nerve and eye, many complication increase day by day; Degree increases the weight of, and is the endocrine metabolism sexual maladjustment of serious harm patient health and life.
How making the blood glucose stable for extended periods of time of diabetic, is the important topic of pendulum in face of the medical scientific research worker, need confirm new medicine through the experiment of science.
Summary of the invention
For these reasons; We are through secular experimentation, from traditional differential diagnosis in tcm opinion method, and modern chemistry composition, bioactivity research angle analysis; Chinese medicine Radix Notoginseng, Radix Codonopsis, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, the Rhizoma Anemarrhenae, Folium Mori extraction are obtained extract, be prepared into hypoglycemic drug.
The object of the invention is to provide a kind of pharmaceutical preparation with hypoglycemic activity.
The present invention also aims to provide a kind of method for preparing of hypoglycemic pharmaceutical preparation.
The present invention realizes through following scheme.
Preparation of the present invention is prepared by following crude drug:
A kind of pharmaceutical preparation with hypoglycemic activity, its crude drug is prepared from: Radix Notoginseng 1-10 weight portion, Radix Codonopsis 1-10 weight portion, Rhizoma Chuanxiong 1-10 weight portion, Radix Salviae Miltiorrhizae 1-5 weight portion, Rhizoma Anemarrhenae 1-5 weight portion, Folium Mori 1-5 weight portion.
Crude drug is prepared from: Radix Notoginseng 3 weight portions, Radix Codonopsis 5 weight portions, Rhizoma Chuanxiong 5 weight portions, Radix Salviae Miltiorrhizae 2 weight portions, the Rhizoma Anemarrhenae 2 weight portions, Folium Mori 2 weight portions.
Its preparation method is:
(1) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water filters, and filtrating is condensed into clear paste;
(2) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water, filter, concentrate, concentrated solution adds ethanol and is 60%-80% to containing the alcohol amount, and hold over night is got supernatant, reclaims ethanol, is condensed into clear paste;
(3) two kinds of clear paste are mixed, drying, grinding and sieving, the powder that gets dry extract is processed solid preparation.
The specific embodiment
One. pharmacological evaluation
Experiment material
Laboratory animal: Japanese purebred large ear rabbit, male and female half and half, female person does not have pregnant, body weight 2kg scholar 0.2kg; Kunming mouse, male and female half and half, body weight 20g scholar 2g; The SD rat, male and female half and half, body weight 100g scholar 10g.
Experiment medicine: solid preparation of the present invention; Diabetes pill, the heavy 0.25g of every ball.
Main agents: alloxan; The adrenalin hydrochloride injection; The blood glucose test kit; Serum insulin radioimmunoassay medicine box.
Key instrument: 721 type spectrophotometers; Put and exempt from detector; Generic centrifuge, three water incubators, automatic biochemistry analyzer.
Experiment 1
Influence to adrenal gland's disposition hyperglycemia mouse blood sugar level
Get healthy mice, body weight 18-22g, male and female half and half are divided into 6 groups at random, 10 every group, are respectively normal control group, model control group, diabetes pill group and the high, medium and low dose groups of solid preparation of the present invention.Normal group and model group are irritated stomach respectively with the 0.25ml/10g normal saline.The diabetes pill group is used the 2.5g/kg diabetes pill; Solid preparation group of the present invention is used 1.5g/kg respectively, solid preparation medicated powder of the present invention.More than 4 groups of suspensions that are mixed with variable concentrations respectively, all roll over the 0.25ml/10g volume.Each organizes all gastric infusion once a day, a continuous week, behind last administration 1h, except that normal group, give the equal lumbar injection epinephrine of all the other each treated animals 0.2mg/kg, and the equal eye socket of all animals is got blood behind the 30min, measures blood sugar concentration with the ortho-aminotoluene method.The result sees table 1
Table 1 solid preparation solid preparation of the present invention is to adrenal gland's disposition hyperglycemia mouse blood sugar level
Shadow noon
Annotate: compare with the model matched group
*P＜0.05
*P＜0.01; Compare △ P＜0.05 △ △ P＜0.01 with the diabetes pill group
Visible by table 1, solid preparation of the present invention can make adrenal gland's disposition hyperglycemia mouse blood sugar level reduce. and compare with diabetes pill, its blood sugar reducing function is slightly excellent.
Experiment 2
Influence to alloxan diabetes man rabbit blood glucose and serum insulin release
Choose healthy rabbits, body weight 1.85-2.15kg, male and female half and half, behind the fasting 12h, auricular vein is injected 5% alloxan normal saline solution 120mg/kg, and the normal control group is left intact.Modeling, all animals be fasting 12h again, and heart extracting blood is measured fasting blood with the ortho-aminotoluene method and warded off fast.The approaching hyperglycemia rabbit of blood sugar level (blood glucose value is 14.11-20.84mmol/L) is chosen in higher and 20 lower rejectings with blood glucose in the modeling rabbit, is divided into group at random, is respectively model control group, diabetes pill group and solid preparation group of the present invention.Normal control group and model control group are used normal saline 5ml/kg; The diabetes pill group is used the 1.5g/kg diabetes pill; Dosage of solid formulations group of the present invention is used 2.4g/kg respectively, solid preparation medicated powder of the present invention.All be mixed with suspension. by the consumption of per kilogram of body weight 5ml, gastric infusion once a day, continuously around.During perfusion, all give normal diet, and note strengthening output.After around the administration completely, fasting 12h measures rabbit fasting glucose (BG) and serum insulin (IRI) value with all animals heart extracting blood.The result sees table 2
Table 2 solid preparation solid preparation of the present invention is to alloxan diabetes rabbit fasting glucose
And the shadow noon of serum insulin levels
Annotate: compare with the model matched group
*P＜0.05
*P＜0.01; Compare △ P＜0.05 △ △ P＜0.01 with the diabetes pill group
3. to the influence of normal mouse fasting glucose
Get healthy mice, body weight 18-22g, male and female half and half are divided 5 groups at random, 10 every group, are respectively normal control group, diabetes pill group and solid preparation group of the present invention.All animals gives normal diet.Each group is irritated stomach medication and dosage with experiment 2.2, gastric infusion once a day, and fasting 12h after continuous 7 days adopts the eye socket blood sampling, and to measure fasting glucose concentration, the result sees table 3
Table 3 solid preparation of the present invention is to the influence of normal mouse fasting blood glucose level
Visible by table 3, each organizes fasting blood sugar through statistical procedures, P＞0.05, and there was no significant difference between group shows that solid preparation of the present invention does not have obvious influence to the normal mouse fasting blood glucose level.
Experimental result: solid preparation of the present invention all has obvious hypoglycemic activity to alloxan diabetes rabbit and adrenal gland's disposition hyperglycemia mice. and the alloxan diabetes rabbit there is the release action of the serum insulin of promotion, and normal mouse blood sugar is not had obvious influence.Pointing out the hypoglycemic main mechanism of solid preparation of the present invention possibly be through to the cytoprotection of beta Cell of islet and promote beta Cell of islet repair function and regeneration to promote β cell uelralante, and unusual carbohydrate metabolism is had inhibition glycogenolysis and glyconeogenesis.
Two. toxicological experiment
1. mice LD50 measures
Get healthy mice, male and female half and half, body weight 18-22g, random packet, 10 every group.Each group is respectively by the dosage of 0.4g/kg, 0.8g/kg, 1.6g/kg, 3.2g/kg, 6.4g/kg, 12.8g/kg (6.25 times, 12.5 times, 25 times, 50 times, 100 times of the dosage that is equivalent to respectively to be grown up; 200 times); Be mixed with respective concentration; Be 0.25ml/10g with amount of liquid medicine, all animals is gastric infusion once a day at every turn, continuous 7 days.Observe during the medication animal activity, diet, two just, equal no abnormality seen such as hair color, figure.Animal was all put to death dissection in the 7th day, observe each important organ and organize also no abnormality seen.Explain that solid preparation of the present invention is far longer than 12.8g/kg (be equivalent to be grown up dosage 220 times) to mice LD50.
2. mice maximum tolerance determination
Get 20 of healthy mices, male and female half and half, body weight 18-22g.With solid preparation medicated powder of the present invention with dissolved in distilled water after, water-soluble to 50% concentration, with gastric infusion behind whole mice fasting 12h, each 0.7ml, every day 2 times, continuous irrigation stomach 7 days.Observation animal activity, diet, two just wait all normal, put to death and dissect animal each important organ of observation and organize also no abnormal.Explain that solid preparation of the present invention is 36g/kg (be equivalent to be grown up dosage 590 times) to the mice maximum tolerated dose.
3. long term toxicity test
Get 80 of healthy rats, body weight 90-110g, male and female half and half are divided into four groups at random, high, medium and low dose groups of solid preparation promptly of the present invention and normal control group, 20 every group.Each group irritate respectively stomach with 4g/kg, 2g/kg, 1g/kg solid preparation medicated powder of the present invention suspension and etc. the dosage normal saline. each 3ml/100g; Every day 1 time; Per 2 weeks weigh 1 time; With the adjustment dosage, successive administration is after 12 weeks, and each treated animal 1-10 sacrificed by decapitation is got hematometry routine blood test and liver function, kidney merit.Dissect perusal main organs form after getting blood; Take out successively and weigh.Calculate and write down dirty body ratio; Take out bladder, get urine from bladder and do routine urianlysis; Main organs is fixed with 10% formalin, and histological examination is done in section.After residue is respectively organized the drug withdrawal of 11-20 animal and observed for two weeks, use above-mentioned steps and method equally, obtain the experiments data.All animals blood, routine urinalysis and liver, renal function compare there was no significant difference all in normal range between group as a result; Each main organs perusal form is no abnormal.Histological examination cellular morphology structure is normal, and no pathologic changes; Dirty body is than each group there was no significant difference.
Experiment conclusion: the acute toxicity testing result shows: mice LD50 is far longer than 12.8g/kg (be equivalent to people's consumption 220 times), and the mice maximum tolerated dose reaches 36g/kg (be equivalent to people's consumption 590 times).The long term toxicity test results suggest: experimental rat routine blood test and liver, renal function there are not obvious influence, harmless to each internal organs of experimental rat.Explain that solid preparation of the present invention does not have obvious toxic-side effects.
Three. preparation embodiment
Embodiment 1
(1) Radix Notoginseng 1kg, Radix Codonopsis 1kg, Rhizoma Chuanxiong 1kg, Radix Salviae Miltiorrhizae 1kg, Rhizoma Anemarrhenae 1kg, Folium Mori 1kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 9 times for the first time; Boil the 1 hour time of carrying, amount of water is 7 times for the second time, boils the 1 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 9 times for the first time, boils the 1 hour time of carrying, and amount of water 6-10 doubly for the second time; Boil the 0.8 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 55% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized, and processes solid preparation.
Embodiment 2
(1) Radix Notoginseng 10kg, Radix Codonopsis 10kg, Rhizoma Chuanxiong 10kg, Radix Salviae Miltiorrhizae 5kg, Rhizoma Anemarrhenae 5kg, Folium Mori 5kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 11 times for the first time; Boil the 1.8 hours time of carrying, amount of water is 9 times for the second time, boils the 1.4 hours time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 11 times for the first time, boils the 1.8 hours time of carrying, and amount of water is 9 times for the second time; Boil the 1.4 hours time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 65% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 3
(1) Radix Notoginseng 3kg, Radix Codonopsis 5kg, Rhizoma Chuanxiong 5kg, Radix Salviae Miltiorrhizae 2kg, Rhizoma Anemarrhenae 2kg, Folium Mori 2kg.
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 10 times for the first time; Boil the 1.5 hours time of carrying, amount of water is 8 times for the second time, boils the 1 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 10 times for the first time, boils the 1.5 hours time of carrying, and amount of water is 8 times for the second time; Boil the 1 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 60% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 4
(1) Radix Notoginseng 6kg, Radix Codonopsis 6kg, Rhizoma Chuanxiong 3kg, Radix Salviae Miltiorrhizae 3kg, Rhizoma Anemarrhenae 1kg, Folium Mori 1kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 8 times for the first time; Boil the 1 hour time of carrying, amount of water is 6 times for the second time, boils the 0.5 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 8 times for the first time, boils the 1 hour time of carrying, and amount of water is 6 times for the second time; Boil the 0.5 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 50% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 5
(1) Radix Notoginseng 9kg, Radix Codonopsis 3kg, Rhizoma Chuanxiong 5kg, Radix Salviae Miltiorrhizae 1kg, Rhizoma Anemarrhenae 2kg, Folium Mori 2kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 12 times for the first time; Boil the 2 hours time of carrying, amount of water is 10 times for the second time, boils the 1.5 hours time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 12 times for the first time, boils the 2 hours time of carrying, and amount of water is 10 times for the second time; Boil the 1.5 hours time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 70% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 6
(1) Radix Notoginseng 3.5kg, Radix Codonopsis 3.5kg, Rhizoma Chuanxiong 4.0kg, Radix Salviae Miltiorrhizae 4.9kg, Rhizoma Anemarrhenae 2.0kg, Folium Mori 2.5kg.
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 10 times for the first time; Boil the 1.5 hours time of carrying, amount of water is 8 times for the second time, boils the 1 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 10 times for the first time, boils the 1.5 hours time of carrying, and amount of water is 8 times for the second time; Boil the 1 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 60% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 7
(1) Radix Notoginseng 1kg, Radix Codonopsis 1kg, Rhizoma Chuanxiong 41kg, Radix Salviae Miltiorrhizae 5kg, Rhizoma Anemarrhenae 5kg, Folium Mori 5kg.
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 10 times for the first time; Boil the 1.5 hours time of carrying, amount of water is 8 times for the second time, boils the 1 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 10 times for the first time, boils the 1.5 hours time of carrying, and amount of water is 8 times for the second time; Boil the 1 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 60% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 8
(1) Radix Notoginseng 10kg, Radix Codonopsis 10kg, Rhizoma Chuanxiong 10kg, Radix Salviae Miltiorrhizae 1kg, Rhizoma Anemarrhenae 3kg, Folium Mori 5kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 8 times for the first time; Boil the 1 hour time of carrying, amount of water is 6 times for the second time, boils the 0.5 hour time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 8 times for the first time, boils the 1 hour time of carrying, and amount of water is 6 times for the second time; Boil the 0.5 hour time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 50% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, vacuum drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Embodiment 9
(1) Radix Notoginseng 5kg, Radix Codonopsis 5kg, Rhizoma Chuanxiong 5kg, Radix Salviae Miltiorrhizae 2.5kg, Rhizoma Anemarrhenae 2.5kg, Folium Mori 2.5kg;
(2) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water 2 times, amount of water is 11 times for the first time; Boil the 1.8 hours time of carrying, amount of water is 9 times for the second time, boils the 1.4 hours time of carrying; Collecting decoction filters, and it is the clear paste of 1.28-1.32 that filtrating is concentrated into 60 ℃ of heat survey relative densities;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water 2 times, amount of water is 11 times for the first time, boils the 1.8 hours time of carrying, and amount of water is 9 times for the second time; Boil the 1.4 hours time of carrying, collecting decoction filters; Being evaporated to 60 ℃ of heat survey relative densities is 1.05-1.10, and adding ethanol is 65% to containing the alcohol amount, hold over night; Get supernatant, reclaim ethanol, being concentrated into 60 ℃ of heat survey relative densities is the clear paste of 1.28-1.32;
(4) two kinds of clear paste are mixed, drying is pulverized the back and is crossed 80 mesh sieves, and the powder that gets dry extract is processed solid preparation.
Claims (3)
1. pharmaceutical preparation with hypoglycemic activity is characterized in that being prepared from following raw material medicaments: Radix Notoginseng 1-10 weight portion, Radix Codonopsis 1-10 weight portion, Rhizoma Chuanxiong 1-10 weight portion, Radix Salviae Miltiorrhizae 1-5 weight portion, Rhizoma Anemarrhenae 1-5 weight portion, Folium Mori 1-5 weight portion.
2. a kind of pharmaceutical preparation with hypoglycemic activity according to claim 1 is prepared from following raw material medicaments: Radix Notoginseng 3 weight portions, Radix Codonopsis 5 weight portions, Rhizoma Chuanxiong 5 weight portions, Radix Salviae Miltiorrhizae 2 weight portions, the Rhizoma Anemarrhenae 2 weight portions, Folium Mori 2 weight portions.
3. a kind of pharmaceutical preparation according to claim 1 with hypoglycemic activity, its preparation method is:
(1) get Radix Notoginseng, Radix Codonopsis, the Rhizoma Anemarrhenae, Folium Mori four Chinese medicine, decocte with water filters, and filtrating is condensed into clear paste;
(2) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae two flavor medicine decocte with water, filter, concentrate, concentrated solution adds ethanol and is 60%-80% to containing the alcohol amount, and hold over night is got supernatant, reclaims ethanol, is condensed into clear paste;
(3) two kinds of clear paste are mixed, drying, grinding and sieving, the powder that gets dry extract is processed solid preparation.
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| CN1387778A (en) * | 2002-06-21 | 2003-01-01 | 郁云生 | Denatured sorghum candy and its use |
Non-Patent Citations (1)
| Title |
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| 屠庆祝，等.浅谈中药治疗糖尿病的用药规律.《中医药临床杂志》.2005,第17卷(第3期),第294-295页. * |
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