CN101584411B - Black cutworm larva artificial feed and preparation method and application thereof - Google Patents
Black cutworm larva artificial feed and preparation method and application thereof Download PDFInfo
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- CN101584411B CN101584411B CN2008101123997A CN200810112399A CN101584411B CN 101584411 B CN101584411 B CN 101584411B CN 2008101123997 A CN2008101123997 A CN 2008101123997A CN 200810112399 A CN200810112399 A CN 200810112399A CN 101584411 B CN101584411 B CN 101584411B
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Abstract
The invention relates to a black cutworm larva artificial feed. The formulation of the feed is prepared from wheat germ, corn powder, casein, sucrose, agar, Weishi salt, vitamin mixed liquor, preservative and water. The invention further relates to a preparation method and application of the feed. The feed has low cost, excellent antiseptic effect, convenient preparation, stable feeding effect andstrong practicability.
Description
Technical field
The present invention relates to a kind of man-made feeds of raising insect, more particularly, the present invention relates to a kind of man-made feeds of extensive raising black cutworm larvae.Simultaneously, the invention still further relates to the compound method and the application thereof of this black cutworm larva artificial feed.
Background technology
Black cutworm Agrotis ypsilon (Rottemberg) belongs to Lepidoptera (Lepidoptera), and Noctuidae (Noctuidae) is a kind of migrating property agricultural pests.Be commonly called as cutworm, cutworm is important subterranean pest-insect, and its host plant reaches 106 kinds.Not only cause harm crops and various vegetables such as corn, Chinese sorghum, millet, broom corn millet, wheat class, cotton, tobacco, potato, sweet potato, sesame, beans, sunflower, clover, crudefiber crop and cause harm nursery stock and multiple ruderals such as each planting fruit-trees, forest, flowers.
In recent years; Because deepening continuously of entomology research; And the development of control of insect technology, need the identical insect of the thousands of physiology of supply, therefore; Need be in the extensive raising of indoor realization insect, this just needs suitable growing environment and the man-made feeds that are fit to replace the external environment and the natural feed of limits insect growth.
At present; The research of the man-made feeds of relevant black cutworm relates to Xin in 1979 prescription raising black cutworm that six usefulness whole wheat embryo 24.0g, the casein 28.0g that does not contain vitamin, sucrose 28.0g, agar 20.0g, Webster salt-mixture 8.0g, sorbic acid 1.6g, Ni Baijin (methyl p-hydroxybenzoate) 1.6g, potassium hydroxide (10%) 16ml, ethanol (95%) 15ml, vitamin mixture 26.4ml, water 650.0ml form that is situated between; Black cutworm larvae that this prescription is raised and development duration thereof have compared with the prolongation of fresh plant raising 5～10 days; Other index and contrast no significant difference, and do not mention indexs such as survival rate of larvae, egg hatching rate in the literary composition.The existing manual feed also has certain deficiency on the antiseptic effect of the survival rate of larva and feed, survival rate of larvae fails to reach more than 80%, and feed corrupt phenomenon promptly occurred in 5 days in the condition of raising held, influenced growing of larva; And, cause larva can't accomplish growth and development process, or produce lopsided pupa and the lopsided adult that sprouts wings with the virus and the appearance of germ contamination.
Summary of the invention
The man-made feeds that the purpose of this invention is to provide a kind of suitable raising black cutworm larvae are to solve the problems referred to above that exist in the prior art.
Another object of the present invention provides a kind of method for preparing black cutworm larva artificial feed.
A further object of the present invention provides the application of black cutworm larva artificial feed in breeding black cutworm larvae.
Of the present invention also have a purpose to provide a kind of method of breeding black cutworm larvae.
The man-made feeds of black cutworm larvae of the present invention, it comprises following raw material by weight:
Wheat germ powder: 10～20
Corn flour: 10～20
Casein: 26～30
Sucrose: 26～30
Agar: 18～22
Webster salt: 7～9
Vitamin mixture: 24～28
Anticorrisive agent: 0.2～9
Potassium hydroxide: 1～1.9
95% ethanol: 14～16
Water: 600-700.
The man-made feeds of black cutworm larvae of the present invention, the preferred following weight parts of wherein said raw material:
Wheat germ powder: 12～18
Corn flour: 12～18
Casein: 26～30
Sucrose: 26～30
Agar: 18～22
Webster salt: 7～9
Vitamin mixture: 24～28
Anticorrisive agent: 3～7
Potassium hydroxide: 1.3～1.7
95% ethanol: 14～16
Water: 620～680.
The man-made feeds of black cutworm larvae of the present invention, the preferred following weight parts of wherein said raw material:
The wheat germ powder: 15,
Corn flour: 15
Casein: 28
Sucrose: 28
Agar: 20
Webster salt: 8
Vitamin mixture: 25
Anticorrisive agent: 6.2
Potassium hydroxide: 1.6
95% ethanol: 15
Water: 620.
Black cutworm larva artificial feed of the present invention, wherein said anticorrisive agent is selected from one or more in sorbic acid, Ni Baijin, the formaldehyde.
Black cutworm larva artificial feed of the present invention, wherein said anticorrisive agent are the mixture of sorbic acid, Ni Baijin, formaldehyde.
Black cutworm larva artificial feed of the present invention, the weight proportion of wherein said anticorrisive agent sorbic acid, Ni Baijin, formaldehyde is 1～2: 1～2: 1～3.
Black cutworm larva artificial feed of the present invention, wherein said vitamin mixture are the Fan Shi vitamin mixtures.
The preparation method of black cutworm larva artificial feed of the present invention, undertaken by following step:
(1) wheat germ is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt, corn flour, sucrose, casein mixing;
(2) agar is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid, Ni Baijin, formaldehyde, potassium hydroxide, 95% ethanol and vitamin mixtures that add in the steps (1).
The invention provides the application of black cutworm larva artificial feed in breeding black cutworm larvae.
The present invention breeds the method for black cutworm larvae, comprises the steps:
(1) man-made feeds is cut into the square fritter of 1cm, puts into 24 orifice plates, insert the larva that just incubates, 2～3 in every hole; Place 26 ± 1 ℃ of temperature, relative humidity 70 ± 5% is under the dark condition;
(2) every at a distance from man-made feeds of replacing in seven days, during 3 ages with black cutworm larvae go to sterilized in vitro until pupating;
(3) adult after the emergence is put into dependent insect cage, the hydromel of feeding.Be coated with gauze above, lay eggs for adult;
(4) repeat above-mentioned 1～3 step after the larva hatching.
The invention has the beneficial effects as follows:
1, this artificial fodder production process is easy, and cost is lower.
2, this artificial feed has been done change on the corrosion-resistant composition, makes the better effects if of man-made feeds on anticorrosion, and the survival rate of larva is higher.
3, adopt after the artificial feed raising of the present invention, satisfied the needs of indoor mass rearing.
The specific embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
(1) wheat germ 24g is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt 8g, sucrose 28g, casein 28g mixing;
(2) agar 20g is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid 1.6g, the golden 1.6g of Buddhist nun uncle, 10% potassium hydroxide 16ml, 95% ethanol 15ml and vitamin mixture 26.4ml that add in the steps (1), promptly gets.
Embodiment 2
(1) wheat germ 18g is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt 8g, corn flour 15g, sucrose 28g, casein 28g mixing;
(2) agar 20g is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid 1.6g, the golden 1.6g of Buddhist nun uncle, 10% potassium hydroxide 16ml, 95% ethanol 15ml and vitamin mixture 26.4ml that add in the steps (1), promptly gets.
Embodiment 3
(1) wheat germ 18g is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt 8g, corn flour 15g, sucrose 28g, casein 28g mixing;
(2) agar 20g is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid 1.6g, the golden 1.6g of Buddhist nun uncle, formaldehyde 3ml, 10% potassium hydroxide 16ml, 95% ethanol 15ml and vitamin mixture 26.4ml that add in the steps (1), promptly gets.
Embodiment 4
(1) wheat germ 10g is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt 7g, corn flour 10g, sucrose 26g, casein 26g mixing;
(2) agar 18g is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid 1g, the golden 1g of Buddhist nun uncle, formaldehyde 1ml, 10% potassium hydroxide 10ml, 95% ethanol 14ml and vitamin mixture 24ml that add in the steps (1), promptly gets.
Embodiment 5
(1) wheat germ 20g is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt 9g, corn flour 20g, sucrose 30g, casein 30g mixing;
(2) agar 22g is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid 1.6g, the golden 1.4g of Buddhist nun uncle, formaldehyde 3ml, 10% potassium hydroxide 19ml, 95% ethanol 16ml and vitamin mixture 28ml that add in the steps (1), promptly gets.
Table 1 man-made feeds prescription
Experimental example
Each worm attitude of black cutworm through under five kinds of man-made feeds (seeing table 1) are raised is respectively observed, and notes the similarities and differences of each the worm attitude life habit under each feed raising, draws some biological indicators shown in the table 2.Test is carried out under the dark condition of relative humidity 70 ± 5% 26 ± 1 ℃ of temperature.
The method of each index test is following:
Survival rate of larvae is measured
The larva that just incubates is chosen on the consistent access man-made feeds of 600 first-born vigor, established three repetitions, each repeats 200, notes the mature larva quantity before pupating, and asks its average, utilizes formula (1) to calculate the larva survival rate.
Larva survival rate (%)=(the survival mature larva number/initial examination larva sum that supplies) * 100... (1)
Percentage of pupation is measured
Choose the larva in the above survival rate of larvae determination test, it is added up and observes, write down three groups of pupa numbers of pupating in repeating respectively.Calculate percentage of pupation according to formula (2).
Percentage of pupation (%)=(the pupa number/initial examination larva number sum that supplies) * 100......... (2)
Average pupa is heavy:
Collect the pupa of being changed in the above percentage of pupation test, it is heavy that average pupa is calculated in weigh by formula (3).
Average pupa weighs=total total number of pupa weight/pupa ... ... ... ... ... ... ... (3)
Eclosion rate is measured:
After measuring percentage of pupation, pupa is packed in the plate, the adult quantity that record is sprouted wings, by formula eclosion rate is calculated in (4).
Eclosion rate (%)=(sprouting wings into borer population/confession examination pupa number) * 100............... (4)
Egg hatching rate is measured
After measuring eclosion rate, with the adult pairing, treat that it lays eggs after, measure the ovum number of hatching.Utilize formula (5) to calculate egg hatching rate.
Egg hatching rate (%)=(the larva sum/sum of laying eggs of hatching) * 100... (5)
Table 2 different component feed is raised the black cutworm larvae result relatively
Visible by table 2 result: as to compare with the feed that does not add corn flour 1; The black cutworm larvae survival rate that the artificial feed of the present invention is raised is significantly increased, and the feed formula that the adds formaldehyde prescription survival rate of larvae, eclosion rate and the egg hatching rate that do not add formaldehyde all has in various degree and improve.
More than further illustrate content of the present invention through embodiment and experimental example, but should not be construed as limitation of the present invention.Those of ordinary skills will be understood that component and preparation method's step or modification or the replacement that condition is done to the artificial feed of the present invention all fall among the scope of the present invention under the situation that does not deviate from the present invention's spirit and essence.
Claims (4)
1. the man-made feeds of a black cutworm larvae is characterized in that, it comprises following raw material by weight:
Wheat germ powder: 15g
Corn flour: 15g
Casein: 28g
Sucrose: 28g
Agar: 20g
Webster salt: 8g
Vitamin mixture: 26.4ml
Sorbic acid: 1.6g
Nipalgin: 1.4g
Formaldehyde: 3ml
10% potassium hydroxide: 16ml
95% ethanol: 15ml
Water: 650ml.
2. with the method for the described formulation black cutworm larvae of claim 1 feed, comprise the steps:
(1) wheat germ is broken into powdery,, add an amount of water and be mixed into pasty state Webster salt, corn flour, sucrose, casein mixing;
(2) agar is joined in the remaining water, be heated to boiling and be thick;
(3) treat that agar is cooled to mixing behind 60～65 ℃ of blending constituent and sorbic acid, nipalgin, formaldehyde, potassium hydroxide, 95% ethanol or vitamin mixtures that add in the steps (1).
3. the application of black cutworm larva artificial feed as claimed in claim 1 in breeding black cutworm larvae.
4. breed the method for black cutworm larvae with the man-made feeds of black cutworm larvae as claimed in claim 1, comprise the steps:
(1) the described man-made feeds of claim 1 are cut into the square fritter of 1cm, put into 24 orifice plates, insert the larva that just incubates, 2～3 in every hole places 26 ± 1 ℃ of temperature, and relative humidity 70 ± 5% is under the dark condition;
(2) every at a distance from man-made feeds of replacing in seven days, during 3 ages with black cutworm larvae go to sterilized in vitro until pupating.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102578425B (en) * | 2012-03-14 | 2014-02-19 | 中国农业科学院植物保护研究所 | Artificial diet for Lepidopterous young larvae, preparation method for artificial diet and application of artificial diet |
| CN103190549B (en) * | 2013-04-28 | 2016-01-20 | 西南大学 | A kind of black cutworm feed and method for breeding thereof |
| CN103404738A (en) * | 2013-08-21 | 2013-11-27 | 北京大北农科技集团股份有限公司 | Artificial feed as well as preparation method and applications thereof |
| CN105192383B (en) * | 2015-09-30 | 2019-03-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of coconut knits moth larvae man-made feeds |
| CN106538902B (en) * | 2016-10-25 | 2018-10-16 | 力源生物科技（广州）有限公司 | A kind of man-made feeds and preparation method thereof of bat moth larvae |
| CN107969576B (en) * | 2017-12-11 | 2021-01-19 | 四川省农业科学院植物保护研究所 | Artificial feed for cantharis larvae, and preparation method and application thereof |
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| CN1682600A (en) * | 2004-12-15 | 2005-10-19 | 中国医学科学院实验动物研究所 | Fodder formulation of constructing nutritive fat animal model |
| CN101181019A (en) * | 2007-12-13 | 2008-05-21 | 济南和美华饲料有限公司 | Feedstuff for pigling replacing milk |
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- 2008-05-23 CN CN2008101123997A patent/CN101584411B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1682600A (en) * | 2004-12-15 | 2005-10-19 | 中国医学科学院实验动物研究所 | Fodder formulation of constructing nutritive fat animal model |
| CN101181019A (en) * | 2007-12-13 | 2008-05-21 | 济南和美华饲料有限公司 | Feedstuff for pigling replacing milk |
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