CN103404738A - Artificial feed as well as preparation method and applications thereof - Google Patents

Artificial feed as well as preparation method and applications thereof Download PDF

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CN103404738A
CN103404738A CN201310367524XA CN201310367524A CN103404738A CN 103404738 A CN103404738 A CN 103404738A CN 201310367524X A CN201310367524X A CN 201310367524XA CN 201310367524 A CN201310367524 A CN 201310367524A CN 103404738 A CN103404738 A CN 103404738A
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parts
grams
mixture
man
made feeds
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杨旭
牛瑞琪
岳健婷
田聪
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BIOTECHNOLOGY CENTER OF BEIJING DABEINONG TECHNOLOGY GROUP Co Ltd
Beijing Dabeinong Technology Group Co Ltd
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BIOTECHNOLOGY CENTER OF BEIJING DABEINONG TECHNOLOGY GROUP Co Ltd
Beijing Dabeinong Technology Group Co Ltd
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Abstract

The invention relates to an artificial feed as well as a preparation method and applications thereof. The artificial feed comprises the following components in parts by weight: 50-80 parts of corn flour, 50-80 parts of soybean meal, 40-60 parts of yeast, 20-50 parts of wheat germ meal, 2-5 parts of casein, 10-30 parts of corn leaf meal, 0.1-1 part of cholesterol, 3-6 parts of a vitamin mixture, 0.2-1.5 parts of choline chloride, 18-22 parts of agar, 12-16 parts of saccharose, 2-5 parts of a preservative and 600-800 parts of water, wherein the preservative is a sorbic acid or nipagin. Because only one preservative is contained in the artificial feed disclosed by the invention, the artificial feed is simple, convenient, safe, corrosion-resistant and comprehensive in nutrition, the breeding period is shortened, and the pupal weight and the survival rate of larvae are improved.

Description

Man-made feeds and its production and use
Technical field
The present invention relates to a kind of man-made feeds and its production and use, particularly relate to man-made feeds of a kind of black cutworm larvae and its production and use.
Background technology
Black cutworm (Agrotis ypsilon Rottemberg), belong to Lepidoptera (Lepidoptera), Noctuidae (Noctuidae).Black cutworm is that in cutworm, distribution is the widest, endangers the heaviest a kind of subterranean pest-insect, is commonly called as cutworm.In China, generally occur, the Yangtze river basin and southeastern coast generating capacity especially abundant with rainfall, that weather is moistening are large.The seedling of the larva various vegetables of harm and crops, bite its stem near the ground broken, makes whole strain death.Black cutworm has the property of migrating. and after three ages, enter the gluttony phase, cause the disconnected ridge that is short of seedling, the serious kind of even ruining, can bring heavy losses to production.
Along with to the deepening continuously of insect Study on Ecophysiological Characteristics of Narrow, and the development of control of insect technology, need to provide the raised growth development condition basically identical for the examination polypide.Therefore, only greatly develop the indoor feeding of insect, could meet entomology research to the demand for the examination polypide; And the exploitation of suitable man-made feeds is the most important things of carrying out the extensive indoor feeding of insect.
At present known black cutworm man-made feeds have (CN101584411B) the improved man-made feeds on the people's such as Mangant basis such as Zhang Yongjun, comprise: 650 milliliters, wheat germ powder 15 grams, corn flour 15 grams, casein 28 grams, sucrose 28 grams, agar 20 grams, Webster salt 8 grams, 26.4 milliliters of vitamin mixtures, sorbic acid 1.6 grams, nipalgin 1.4 grams, 3 milliliters, formaldehyde, 16 milliliters, 10% potassium hydroxide, 15 milliliters of 95% ethanol and water.Although above-mentioned man-made feeds reach more than 80% in the survival rate of having done improvement and larva on antiseptic effect, but still have following defect:
(1) need to add Determination of Preservatives.The anticorrisive agent such as sorbic acid and nipalgin is used and too much not only to insect, can produce toxic and side effect, but also can cause to the experimenter of Long Term Contact formaldehyde the harm such as stimulation, sensitization, mutagenesis is even dead.
(2) breeding cycle is longer, while supplying with in a large number at short notice the worm source for various experiment, may have the problems such as supply deficiency;
(3) need to add the Determination of Preservatives of certain proportioning, the preparation process relative complex.
Summary of the invention
The purpose of this invention is to provide a kind of man-made feeds and its production and use, effectively overcome the prior art anticorrisive agent too much, breeding cycle technological deficiencies such as length, process for preparation relative complex.
For achieving the above object, the invention provides a kind of man-made feeds, the constituent that comprises following parts by weight: corn flour 50-80 part, soy meal 50-80 part, yeast 40-60 part, wheat germ powder 20-50 part, casein 2-5 part, maize leaves powder 10-30 part, cholesterol 0.1-1 part, vitamin mixtures 3-6 part, Choline Chloride 0.2-1.5 part, agar 18-22 part, sucrose 12-16 part, anticorrisive agent 2-5 part and water 600-800 part, described anticorrisive agent is sorbic acid or nipalgin.
Further, described man-made feeds comprise the constituent of following parts by weight: corn flour 55-70 part, soy meal 60-75 part, yeast 45-55 part, wheat germ powder 25-40 part, casein 3-4 part, maize leaves powder 15-20 part, cholesterol 0.3-0.7 part, vitamin mixtures 4-5 part, Choline Chloride 0.5-1 part, agar 18-22 part, sucrose 12-14 part, anticorrisive agent 3-4 part and water 650-750 part, described anticorrisive agent is sorbic acid or nipalgin.
Further, described man-made feeds comprise the constituent of following parts by weight: 730 parts, 60 parts of corn flour, 65 parts of soy meals, 50 parts, yeast, 30 parts, wheat germ powder, 3.3 parts of casein, 20 parts, maize leaves powder, 0.5 part, cholesterol, 4.6 parts of vitamin mixtures, 0.7 part of Choline Chloride, 20 parts, agar, 13 parts of sucrose, 3.3 parts of anticorrisive agents and water, described anticorrisive agent is sorbic acid or nipalgin.
On the basis of technique scheme, described anticorrisive agent is sorbic acid.
Preferably, described vitamin mixtures comprises vitamin C and vitamin B compound.
For achieving the above object, the present invention also provides a kind of preparation method of described man-made feeds, comprising:
Corn flour, soy meal, yeast, wheat germ powder, casein and maize leaves powder are mixed into to the first mixture;
Agar is added in water, is heated to add again sucrose after boiling, be mixed into the second mixture;
Described the second mixture and described the first mixture, anticorrisive agent, vitamin mixtures, cholesterol and Choline Chloride are mixed.
For achieving the above object, the present invention also provides a kind of method of raising black cutworm, comprising: with the described man-made feeds black cutworm of feeding.
Further, at temperature 26-28 ℃, relative humidity 65-75%, under photoperiod L:D=24:0 condition, with the described man-made feeds black cutworm newly hatched larvae of feeding.
For achieving the above object, the present invention also provides the purposes of a kind of described man-made feeds in raising black cutworm.
The invention provides a kind of man-made feeds and its preparation method and application, have the following advantages:
1, simple and convenient.The artificial feed of the present invention only adds a kind of anticorrisive agent, need not consider the proportioning between anticorrisive agent, and the preparation method is simple, is easy to grasp.
2, safety is anticorrosion.The artificial feed of the present invention only adds a kind of anticorrisive agent, can effectively reduce the toxic and side effect that anticorrisive agent produces insect, if only adopt sorbic acid or nipalgin, more can avoid the harm such as stimulation, sensitization, sudden change that the experimenter causes because of Long Term Contact formaldehyde be even dead; The artificial feed of the present invention simultaneously still can reach good antiseptic effect.
3, shorten breeding cycle.The artificial feed of the present invention can solve the experiment demand of supplying in a large number at short notice the worm source.
4, comprehensive nutrition.The artificial feed of the present invention has improved formula and content thereof, makes its nutrition more comprehensive, thereby the pupa of black cutworm heavily waits physical signs more outstanding; Because the artificial feed of the present invention only adds a kind of anticorrisive agent, reduce anticorrisive agent to the toxic and side effect that insect produces simultaneously, increased substantially survival rate of larvae.
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
The accompanying drawing explanation
Fig. 1 is preparation method's flow chart of the artificial feed of the present invention.
The specific embodiment
Below by specific embodiment, further illustrate the technical scheme of the artificial feed of the present invention.
Artificial feed the first embodiment of the present invention (feed 1)
Man-made feeds comprise following constituent: 800 milliliters, corn flour 50 grams, soy meal 80 grams, yeast 40 grams, wheat germ powder 50 grams, casein 2 grams, maize leaves powder 30 grams, cholesterol 0.1 gram, vitamin C 4 grams, vitamin B compound 2 grams, Choline Chloride 0.2 gram, agar 22 grams, sucrose 12 grams, sorbic acid 5 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 26 ℃ of temperature, relative humidity 65%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
Artificial feed the second embodiment of the present invention (feed 2)
Man-made feeds comprise following constituent: 730 milliliters, corn flour 60 grams, soy meal 65.4 grams, yeast 50 grams, wheat germ powder 30 grams, casein 3.3 grams, maize leaves powder 19.6 grams, cholesterol 0.5 gram, vitamin C 3.3 grams, vitamin B compound 1.3 grams, Choline Chloride 0.7 gram, agar 19.6 grams, sucrose 13.1 grams, sorbic acid 3.3 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 27 ℃ of temperature, relative humidity 70%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
The artificial feed of the present invention the 3rd embodiment (feed 3)
Man-made feeds comprise following constituent: 600 milliliters, corn flour 80 grams, soy meal 50 grams, yeast 60 grams, wheat germ powder 20 grams, casein 5 grams, maize leaves powder 10 grams, cholesterol 1 gram, Catergen .1 gram, vitamin B compound 0.9 gram, Choline Chloride 1.5 grams, agar 18 grams, sucrose 16 grams, sorbic acid 2 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 28 ℃ of temperature, relative humidity 75%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
The artificial feed of the present invention the 4th embodiment (feed 4)
Man-made feeds comprise following constituent: 800 milliliters, corn flour 50 grams, soy meal 80 grams, yeast 40 grams, wheat germ powder 50 grams, casein 2 grams, maize leaves powder 30 grams, cholesterol 0.1 gram, vitamin C 4 grams, vitamin B compound 2 grams, Choline Chloride 0.2 gram, agar 22 grams, sucrose 12 grams, nipalgin 5 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 26 ℃ of temperature, relative humidity 65%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
The artificial feed of the present invention the 5th embodiment (feed 5)
Man-made feeds comprise following constituent: 730 milliliters, corn flour 60 grams, soy meal 65.4 grams, yeast 50 grams, wheat germ powder 30 grams, casein 3.3 grams, maize leaves powder 19.6 grams, cholesterol 0.5 gram, vitamin C 3.3 grams, vitamin B compound 1.3 grams, Choline Chloride 0.7 gram, agar 19.6 grams, sucrose 13.1 grams, nipalgin 3.3 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 27 ℃ of temperature, relative humidity 70%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
The artificial feed of the present invention the 6th embodiment (feed 6)
Man-made feeds comprise following constituent: 600 milliliters, corn flour 80 grams, soy meal 50 grams, yeast 60 grams, wheat germ powder 20 grams, casein 5 grams, maize leaves powder 10 grams, cholesterol 1 gram, Catergen .1 gram, vitamin B compound 0.9 gram, Choline Chloride 1.5 grams, agar 18 grams, sucrose 16 grams, nipalgin 2 grams and water.
The method of raising black cutworm comprises with the above-mentioned man-made feeds black cutworm of feeding.Be specially: 28 ℃ of temperature, relative humidity 75%, under photoperiod L:D=24:0 condition, with the above-mentioned man-made feeds black cutworm newly hatched larvae of feeding.
The purposes of a kind of above-mentioned man-made feeds in raising black cutworm.
In the invention described above man-made feeds the first embodiment to the six embodiment, vitamin B compound comprises 3.0% vitamin B1,1.5% vitamin B2,0.2% vitamin B6,10% niacinamide and 1.0% calcium pantothenate, and vitamin B compound can be commercially available medical tablet.
Below by specific embodiment, further illustrate the preparation method's of the artificial feed of the present invention technical scheme.Fig. 1 is preparation method's flow chart of the artificial feed of the present invention, is specially:
Step B1, corn flour, soy meal, yeast, wheat germ powder, casein and maize leaves powder are mixed into to the first mixture;
Step B2, agar is added in water, is heated to add again sucrose after boiling, be mixed into the second mixture;
Step B3, described the second mixture and described the first mixture, anticorrisive agent, vitamin mixtures, cholesterol and Choline Chloride are mixed.
Preparation method first embodiment of the artificial feed of the present invention
Step B11, corn flour 50 grams, soy meal 80 grams, yeast 40 grams, wheat germ powder 50 grams, casein 2 grams and maize leaves powder 30 grams are mixed, obtain the first mixture;
Step B12, cholesterol 0.1 gram, vitamin C 4 grams, vitamin B compound 2 grams and Choline Chloride 0.2 gram are added in 50 ml waters and dissolve, obtain the 3rd mixture;
Step B13, agar 22 grams are added in 750 ml waters, after being heated to boiling, then add sucrose 12 grams, mix it is fully dissolved, obtain the second mixture;
Step B14, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B15, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B16, sorbic acid 5 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B17, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
Preparation method second embodiment of the artificial feed of the present invention
Step B21, corn flour 60 grams, soy meal 65.4 grams, yeast 50 grams, wheat germ powder 30 grams, casein 3.3 grams and maize leaves powder 19.6 grams are mixed, obtain the first mixture;
Step B22, cholesterol 0.5 gram, vitamin C 3.3 grams, vitamin B compound 1.3 grams and Choline Chloride 0.7 gram are added in 30 ml waters and dissolve, obtain the 3rd mixture;
Step B23, agar 19.6 grams are added in 700 ml waters, after being heated to boiling, then add sucrose 13.1 grams, mix it is fully dissolved, obtain the second mixture;
Step B24, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B25, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B26, sorbic acid 3.3 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B27, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
The preparation method of the artificial feed of the present invention the 3rd embodiment
Step B31, corn flour 80 grams, soy meal 50 grams, yeast 60 grams, wheat germ powder 20 grams, casein 5 grams and maize leaves powder 10 grams are mixed, obtain the first mixture;
Step B32, cholesterol 1 gram, Catergen .1 gram, vitamin B compound 0.9 gram and Choline Chloride 1.5 grams are added in 20 ml waters and dissolve, obtain the 3rd mixture;
Step B33, agar 18 grams are added in 580 ml waters, after being heated to boiling, then add sucrose 16 grams, mix it is fully dissolved, obtain the second mixture;
Step B34, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B35, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B36, sorbic acid 2 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B37, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
The preparation method of the artificial feed of the present invention the 4th embodiment
Step B41, corn flour 50 grams, soy meal 80 grams, yeast 40 grams, wheat germ powder 50 grams, casein 2 grams and maize leaves powder 30 grams are mixed, obtain the first mixture;
Step B42, cholesterol 0.1 gram, vitamin C 4 grams, vitamin B compound 2 grams and Choline Chloride 0.2 gram are added in 50 ml waters and dissolve, obtain the 3rd mixture;
Step B43, agar 22 grams are added in 750 ml waters, after being heated to boiling, then add sucrose 12 grams, mix it is fully dissolved, obtain the second mixture;
Step B44, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B45, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B46, nipalgin 5 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B47, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
The preparation method of the artificial feed of the present invention the 5th embodiment
Step B51, corn flour 60 grams, soy meal 65.4 grams, yeast 50 grams, wheat germ powder 30 grams, casein 3.3 grams and maize leaves powder 19.6 grams are mixed, obtain the first mixture;
Step B52, cholesterol 0.5 gram, vitamin C 3.3 grams, vitamin B compound 1.3 grams and Choline Chloride 0.7 gram are added in 30 ml waters and dissolve, obtain the 3rd mixture;
Step B53, agar 19.6 grams are added in 700 ml waters, after being heated to boiling, then add sucrose 13.1 grams, mix it is fully dissolved, obtain the second mixture;
Step B54, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B55, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B56, nipalgin 3.3 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B57, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
The preparation method of the artificial feed of the present invention the 6th embodiment
Step B61, corn flour 80 grams, soy meal 50 grams, yeast 60 grams, wheat germ powder 20 grams, casein 5 grams and maize leaves powder 10 grams are mixed, obtain the first mixture;
Step B62, cholesterol 1 gram, Catergen .1 gram, vitamin B compound 0.9 gram and Choline Chloride 1.5 grams are added in 20 ml waters and dissolve, obtain the 3rd mixture;
Step B63, agar 18 grams are added in 580 ml waters, after being heated to boiling, then add sucrose 16 grams, mix it is fully dissolved, obtain the second mixture;
Step B64, the first mixture and the second mixture are mixed, obtain 4 mixture;
Step B65, by 4 mixture under 121 ℃ sterilizing 30 minutes;
Step B66, nipalgin 2 grams are added to rapidly in the 4 mixture after sterilizing, stir evenly and obtain the 5th mixture;
Step B67, treat that described the 5th mixture is cooled to 50 ℃, be mixed evenly with described the 3rd mixture.
In preparation method first embodiment to the six embodiment of the invention described above man-made feeds, vitamin B compound comprises 3.0% vitamin B1,1.5% vitamin B2,0.2% vitamin B6,10% niacinamide and 1.0% calcium pantothenate, and vitamin B compound can be commercially available medical tablet.
Technique effect below by the concrete artificial feed of experimental evaluation the present invention.
By each worm state of black cutworm that the man-made feeds to different formulations are raised respectively, observe, obtain the experimental result of biological indicator as shown in table 1.
The larva just incubated is chosen on the consistent access man-made feeds of 540 first-born vigor to 27 ± 1 ℃ of temperature, relative humidity 70 ± 5%, photoperiod L:D=24:0.If 3 times are repeated, each repeats 180.Biological indicator and method of testing thereof are as follows:
1, survival rate of larvae
Mature larva quantity before record is pupated, ask its average.
Survival rate of larvae (%)=(the mature larva number of survival/confession examination larva sum) * 100
2, percentage of pupation
Choose the larva in above survival rate of larvae determination test, the pupa number that record is pupated.
Percentage of pupation (%)=(number of pupating/for examination larva sum) * 100
3, average pupa weight
Collect the pupa of changing in above percentage of pupation test, calculate average pupa weight.
Heavy=total pupa of average pupa weigh/pupates to count
4, eclosion rate
After measuring percentage of pupation, pupa is packed in culture dish, the adult quantity after record is sprouted wings.
Eclosion rate (%)=(the one-tenth borer population after the emergence/number of pupating) * 100
5, egg hatching rate
After measuring eclosion rate, by the adult pairing, after it is laid eggs, collect and measure the ovum number of hatching.
Egg hatching rate (%)=(the larva number of the hatching/sum of laying eggs) * 100
Feed 1 is to the constituent of feed 6 constituent of artificial feed the first embodiment to the six embodiment of corresponding the present invention respectively, and known feed comprises following constituent: 650 milliliters, wheat germ powder 15 grams, corn flour 15 grams, sucrose 28 grams, casein 28 grams, agar 20 grams, Webster salt 8 grams, 26.4 milliliters of normal form vitamin mixtures, sorbic acid 1.6 grams, nipalgin 1.4 grams, 3 milliliters, formaldehyde, 16 milliliters, 10% potassium hydroxide, 15 milliliters of 95% ethanol and water.
Table 1, different component man-made feeds are raised the experimental result of black cutworm larvae
Figure BDA0000369952880000081
The experimental result of table 1 shows: the artificial feed of the present invention is due to comprehensive nutrition and only added a kind of anticorrisive agent, with known feed, compare, not only make the artificial feed of the present invention still have the corrosion-resistant effect, and the survival rate of larvae of the black cutworm of the artificial feed raising of the present invention, percentage of pupation, pupa weight, eclosion rate and egg hatching rate all have raising in various degree.
In sum, artificial feed of the present invention and its production and use only adds a kind of anticorrisive agent, need not consider the proportioning between anticorrisive agent, and the preparation method is simple, is easy to grasp; The toxic and side effect of anticorrisive agent to people and insect generation can be effectively reduced simultaneously, and still good antiseptic effect can be reached; And, due to comprehensive nutrition, make the physical signs such as the heavy and survival rate of larvae of the pupa of black cutworm more outstanding; Also solved the experiment demand of supplying in a large number at short notice the worm source.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not break away from the spirit and scope of technical solution of the present invention.

Claims (9)

1. man-made feeds, it is characterized in that, the constituent that comprises following parts by weight: corn flour 50-80 part, soy meal 50-80 part, yeast 40-60 part, wheat germ powder 20-50 part, casein 2-5 part, maize leaves powder 10-30 part, cholesterol 0.1-1 part, vitamin mixtures 3-6 part, Choline Chloride 0.2-1.5 part, agar 18-22 part, sucrose 12-16 part, anticorrisive agent 2-5 part and water 600-800 part, described anticorrisive agent is sorbic acid or nipalgin.
2. man-made feeds according to claim 1, it is characterized in that, the constituent that comprises following parts by weight: corn flour 55-70 part, soy meal 60-75 part, yeast 45-55 part, wheat germ powder 25-40 part, casein 3-4 part, maize leaves powder 15-20 part, cholesterol 0.3-0.7 part, vitamin mixtures 4-5 part, Choline Chloride 0.5-1 part, agar 18-22 part, sucrose 12-14 part, anticorrisive agent 3-4 part and water 650-750 part, described anticorrisive agent is sorbic acid or nipalgin.
3. man-made feeds according to claim 2, it is characterized in that, the constituent that comprises following parts by weight: 730 parts, 60 parts of corn flour, 65.4 parts of soy meals, 50 parts, yeast, 30 parts, wheat germ powder, 3.3 parts of casein, 19.6 parts, maize leaves powder, 0.5 part, cholesterol, 4.6 parts of vitamin mixtures, 0.7 part of Choline Chloride, 19.6 parts, agar, 13.1 parts of sucrose, 3.3 parts of anticorrisive agents and water, described anticorrisive agent is sorbic acid or nipalgin.
4. according to the described man-made feeds of claim 1-3 any one, it is characterized in that, described anticorrisive agent is sorbic acid.
5. according to the described man-made feeds of claim 1-4 any one, it is characterized in that, described vitamin mixtures comprises vitamin C and vitamin B compound.
6. the preparation method of the described man-made feeds of claim 1-5 any one, is characterized in that, comprising:
Corn flour, soy meal, yeast, wheat germ powder, casein and maize leaves powder are mixed into to the first mixture;
Agar is added in water, is heated to add again sucrose after boiling, be mixed into the second mixture;
Described the second mixture and described the first mixture, anticorrisive agent, vitamin mixtures, cholesterol and Choline Chloride are mixed.
7. a method of raising black cutworm, is characterized in that, comprising: with the described man-made feeds of the claim 1-5 any one black cutworm of feeding.
8. raise according to claim 7 the method for black cutworm, it is characterized in that, comprising: at temperature 26-28 ℃, relative humidity 65-75%, under photoperiod L:D=24:0 condition, with the described man-made feeds black cutworm newly hatched larvae of feeding.
9. described man-made feeds of the claim 1-5 any one purposes in raising black cutworm.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105192383A (en) * 2015-09-30 2015-12-30 中国热带农业科学院环境与植物保护研究所 Artificial feed for opisina arenosella walker larvae
CN105192383B (en) * 2015-09-30 2019-03-29 中国热带农业科学院环境与植物保护研究所 A kind of coconut knits moth larvae man-made feeds
CN106974140A (en) * 2017-03-22 2017-07-25 广东人为峰健康管理有限公司 A kind of high nutrition drosophila man-made feeds and preparation method thereof
CN108740611A (en) * 2018-05-24 2018-11-06 河南省农业科学院植物保护研究所 Dichocrocis punctiferalis larva artificial feed, preparation method and dichocrocis punctiferalis method for breeding
CN108740611B (en) * 2018-05-24 2021-10-29 河南省农业科学院植物保护研究所 Artificial feed for dichocrocis punctiferalis larvae, preparation method of artificial feed and dichocrocis punctiferalis breeding method

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Application publication date: 20131127