CN101580586B - 一种聚谷氨酸提取方法 - Google Patents
一种聚谷氨酸提取方法 Download PDFInfo
- Publication number
- CN101580586B CN101580586B CN2009100202214A CN200910020221A CN101580586B CN 101580586 B CN101580586 B CN 101580586B CN 2009100202214 A CN2009100202214 A CN 2009100202214A CN 200910020221 A CN200910020221 A CN 200910020221A CN 101580586 B CN101580586 B CN 101580586B
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- CN
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- Prior art keywords
- colloid
- ethanol
- liquid
- polyglutamic acid
- stirring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 229920002643 polyglutamic acid Polymers 0.000 title claims abstract description 34
- 108010020346 Polyglutamic Acid Proteins 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 77
- 239000000084 colloidal system Substances 0.000 claims abstract description 33
- 150000003839 salts Chemical class 0.000 claims abstract description 15
- 108700022290 poly(gamma-glutamic acid) Proteins 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims abstract description 13
- 239000012535 impurity Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 108091005804 Peptidases Proteins 0.000 claims abstract description 6
- 239000000839 emulsion Substances 0.000 claims abstract description 5
- 239000003292 glue Substances 0.000 claims abstract description 5
- 159000000000 sodium salts Chemical class 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 47
- 238000003756 stirring Methods 0.000 claims description 33
- 238000000926 separation method Methods 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 10
- 239000003513 alkali Substances 0.000 claims description 9
- 238000001556 precipitation Methods 0.000 claims description 9
- 239000002893 slag Substances 0.000 claims description 9
- 238000000108 ultra-filtration Methods 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 8
- 238000005189 flocculation Methods 0.000 claims description 8
- 230000016615 flocculation Effects 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 238000012546 transfer Methods 0.000 claims description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 claims description 5
- 102000035195 Peptidases Human genes 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 238000000502 dialysis Methods 0.000 claims description 5
- 239000000049 pigment Substances 0.000 claims description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 241000237502 Ostreidae Species 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- 235000020636 oyster Nutrition 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000001291 vacuum drying Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 2
- 102000016943 Muramidase Human genes 0.000 abstract 1
- 108010014251 Muramidase Proteins 0.000 abstract 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 241001052560 Thallis Species 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 239000004325 lysozyme Substances 0.000 abstract 1
- 229960000274 lysozyme Drugs 0.000 abstract 1
- 235000010335 lysozyme Nutrition 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000009777 vacuum freeze-drying Methods 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 28
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229960002989 glutamic acid Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009100202214A CN101580586B (zh) | 2009-03-30 | 2009-03-30 | 一种聚谷氨酸提取方法 |
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CN2009100202214A CN101580586B (zh) | 2009-03-30 | 2009-03-30 | 一种聚谷氨酸提取方法 |
Publications (2)
Publication Number | Publication Date |
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CN101580586A CN101580586A (zh) | 2009-11-18 |
CN101580586B true CN101580586B (zh) | 2010-12-29 |
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CN2009100202214A Expired - Fee Related CN101580586B (zh) | 2009-03-30 | 2009-03-30 | 一种聚谷氨酸提取方法 |
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CN (1) | CN101580586B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101857673B (zh) * | 2010-05-31 | 2012-05-23 | 杭州普济医药技术开发有限公司 | 从发酵液中分离纯化γ-聚谷氨酸的方法 |
CN102702508B (zh) * | 2012-05-24 | 2013-10-16 | 领先生物农业股份有限公司 | 一种以工业规模提取聚谷氨酸的方法 |
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2009
- 2009-03-30 CN CN2009100202214A patent/CN101580586B/zh not_active Expired - Fee Related
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CN101580586A (zh) | 2009-11-18 |
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Owner name: INNER MONGOLIA FUFENG BIOTECHNOLOGIES CO., LTD. Free format text: FORMER OWNER: SHANDONG FUFENG BIOLOGY SCIENCE + TECHNOLOGY DEVELOPMENT CO., LTD. Effective date: 20110728 |
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Free format text: CORRECT: ADDRESS; FROM: 276600 LINYI, SHANDONG PROVINCE TO: 010070 HOHHOT, INNER MONGOLIA AUTONOMOUS REGION |
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Effective date of registration: 20110728 Address after: 010070 Inner Mongolia Hohhot economic and Technological Development Zone, South Jinchuan district two road Patentee after: INNER MONGOLIA FUFENG BIOTECHNOLOGIES Co.,Ltd. Address before: 276600 Shandong city of Linyi province Junan County Long Road North Patentee before: Shandong Fufeng Bio-Technology Development Co.,Ltd. |
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