CN101575626A - Extraction method of water-soluble spirulina polysaccharide - Google Patents
Extraction method of water-soluble spirulina polysaccharide Download PDFInfo
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- CN101575626A CN101575626A CNA2008100158804A CN200810015880A CN101575626A CN 101575626 A CN101575626 A CN 101575626A CN A2008100158804 A CNA2008100158804 A CN A2008100158804A CN 200810015880 A CN200810015880 A CN 200810015880A CN 101575626 A CN101575626 A CN 101575626A
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- spirulina
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- spirulina polysaccharide
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Abstract
The invention relates to an extraction method of water-soluble spirulina polysaccharide, comprising the following steps: soaking a raw spirulina into water, stirring and putting the solution into water at a temperature of 20 DEG C below zero to freeze for 48 hours, taking out the frozen matter to rapidly melt, centrifugate and filter, remaining the centrifugating liquid to extract phycobiliprotein, extracting spirulina dreg to extract the spirulina polysaccharide; using HCl to adjust the extracted spirulina dreg to have a pH value of 3 to 10 at a temperature of 35 to 65 DEG C, adding complex enzyme for zymohydrolysis, sterilizing, filtering and separating a zymolytic solution, adding K2CO3 to the filtration residual to dissolve the filtration dreg into a solution, centrifugating and filtering the solution, discarding the filtration dreg and remaining the filtration dreg solution centrifugating liquid; and mixing the zymolytic solution and the filtration dreg solution centrifugating liquid, adjusting the mixture to have a neutral pH value and concentrating, spraying and drying the mixture into the water-soluble spirulina polysaccharide. The extraction method of the water-soluble spirulina polysaccharide has the following advantages that firstly the spirulina polysaccharide is water-soluble powder so as to be convenient to use and easy to absorb; secondly, the water-soluble powder is further processed so as to be used as a raw material of food additives, functional foods and medicaments, and the like, thereby having wide serviceable range; thirdly, the spirulina polysaccharide basically remains various original nutrient contents of the spirulina except spirulina protein; and fourthly, the pretreated centrifugating liquid in the extraction process can be used for extracting the phycobiliprotein so as to improve the utilization rate of the spirulina.
Description
Technical field
The present invention relates to biological technical field, particularly the extracting method of water-soluble spirulina polysaccharide.
Background technology
Spirulina is the blue-green algae in little algae, it has high protein, lower fat, low sugar, the characteristics of low-cholesterol, contain multivitamin, protein, fat, carbohydrate, mineral substance and trace element, and various ratios are scientific and reasonable, wherein a lot of compositions reach content of medicines and intensity, make its medicinal function very extensive, promoting human body metabolism, enhance immunity power, improve body constitution, radioprotective, auxiliary cancer is put, chemotherapy, reduce radiotherapy, many-sides such as chemotherapy side effect all have active effect, are the active heath food of a kind of ideal pure-natural biological.Therefore be recommended as " optimal food of 21st century " by Food and Argriculture OrganizationFAO and world's food association of United Nations.
Polysaccharide in the spirulina can strengthen the proliferation of bone marrow cells vigor, promote DNA to repair Synthesis, the good resistance radiation function is arranged and reduce radiotherapy, chemotherapy side effect, ionization radiation injury had tangible protection effect, can improve the nonspecific cellular immune function of body, improve the ability of resisting germ, poisoning intrusion, promote immune organ growths such as thymus gland, spleen gland, promote gpv protein synthetic, eliminate immunosuppressor immune restraining effect.
Existing in the market spirulina product is a lot, but specially at spirulina polysaccharide but seldom, water-soluble spirulina polysaccharide especially.
Summary of the invention
The extracting method that the purpose of this invention is to provide a kind of water-soluble spirulina polysaccharide, this extracting method preparation and isolated spirulina polysaccharide are water solube powder, absorb easily, and operating process is simple, nutritive ingredient is except that algae albumen in the spirulina polysaccharide that extracts, substantially kept original each nutritive ingredient of spirulina, pretreated centrifugate can be retained the extraction phycobiliprotein, has improved the utilization ratio of spirulina raw material.
The objective of the invention is to be achieved through the following technical solutions: a kind of extracting method of water-soluble spirulina polysaccharide may further comprise the steps:
A. the pre-treatment of spirulina raw material is immersed spirulina raw material in the water, is placed in the water of temperature-20 ℃ freezing 48 hours after stirring evenly, and takes out quick thawing, centrifugal filtration, and centrifugate is retained and is extracted phycobiliprotein, and algae-residue extracts spirulina polysaccharide;
B. the algae-residue that the extraction of spirulina polysaccharide, separation will be extracted in the steps A is regulated pH value at 3-10 with HCL, and temperature is at 35 ℃--and 65 ℃, add complex enzyme zymohydrolysis, carry out inactivation treatment, filter to isolate enzymolysis solution, add K in the filter residue
2CO
3Be dissolved into solution, centrifugal filtration discards the Lu slag, keeps Lu slag dissolving centrifugate;
C. enzymolysis solution and Lu slag dissolving centrifugate among the finished product dry mixed step B is regulated PH to neutral, concentrate, spraying drying water-soluble spirulina polysaccharide.
In technique scheme, PH is controlled at 3-10 during enzymolysis, and its temperature is controlled at 35 ℃-65 ℃.
In technique scheme, its PH is controlled at 4-6 during enzymolysis, and temperature is controlled at 40 ℃-55 ℃.
In technique scheme, PH is 5.0 during enzymolysis, and temperature is 50 ℃.
In technique scheme, its main component of prozyme used during enzymolysis is cellulase, proteolytic enzyme, and the vigor of two kinds of enzymes respectively is 50000 unit meters.
The present invention compared with prior art, its advantage is: one, spirulina polysaccharide is water solube powder, and is easy to use, absorbs easily; Two, further deep processing on the water solube powder basis can be used as the raw material of foodstuff additive, functional food and medicine etc., and use range is extensive; Three, nutritive ingredient has kept original each nutritive ingredient of spirulina substantially in the spirulina polysaccharide except that algae albumen; Four, pretreated centrifugate can be extracted phycobiliprotein in the leaching process, improves the utilization ratio of spirulina.
Embodiment
Take by weighing spirulina raw material 10kg, add 10 times of water, stirred evenly under-20 ℃ of temperature freezing 48 hours, fast the centrifugal Shanghai of crossing after the thawing, centrifugate is retained, algae-residue adds 5 times of water, transfers PH=6.2, adds 4-6% prozyme (cellulase, proteolytic enzyme etc., the vigor of enzyme respectively is 50000 unit meters) 50 ℃ of following enzymolysis of temperature 12 hours, under 80 ℃, carry out deactivation half an hour then, filter to isolate enzymolysis solution, filter residue is added K
2CO
3Be dissolved into solution, centrifugal filtration discards the Lu slag, and mixed enzymolysis solution is regulated PH to neutral, concentrate, spraying drying water-soluble spirulina polysaccharide.
Claims (5)
1, a kind of extracting method of water-soluble spirulina polysaccharide may further comprise the steps:
A. the pre-treatment of spirulina raw material is immersed spirulina raw material in the water, is placed in the water of temperature-20 ℃ freezing 48 hours after stirring evenly, and takes out quick thawing, centrifugal filtration, and centrifugate is retained and is extracted phycobiliprotein, and algae-residue extracts spirulina polysaccharide;
B. the algae-residue that the extraction of spirulina polysaccharide, separation will be extracted in the steps A is regulated pH value at 3-10 with HCL, and temperature is at 35 ℃--and 65 ℃, add complex enzyme zymohydrolysis, carry out inactivation treatment, filter to isolate enzymolysis solution, add K in the filter residue
2CO
3Be dissolved into solution, centrifugal filtration discards the Lu slag, keeps Lu slag dissolving centrifugate;
C. enzymolysis solution and Lu slag dissolving centrifugate among the finished product dry mixed step B is regulated PH to neutral, concentrate, spraying drying water-soluble spirulina polysaccharide.
2, according to the extracting method of claim 1 described water-soluble spirulina polysaccharide, PH is controlled at 3-10 when it is characterized in that enzymolysis, and its temperature is controlled at 35 ℃-65 ℃.
3, according to the extracting method of claim 1 described water-soluble spirulina polysaccharide, its PH is controlled at 4-6 when it is characterized in that enzymolysis, and temperature is controlled at 40 ℃-55 ℃.
4, according to the extracting method of claim 1 described water-soluble spirulina polysaccharide, PH is 5.0 during its feature enzymolysis, and temperature is 50 ℃.
5, according to the extracting method of claim 1 described water-soluble spirulina polysaccharide, its main component of prozyme used when it is characterized in that enzymolysis is cellulase, proteolytic enzyme, and the vigor of two kinds of enzymes respectively is 50000 unit meters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100158804A CN101575626A (en) | 2008-05-06 | 2008-05-06 | Extraction method of water-soluble spirulina polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100158804A CN101575626A (en) | 2008-05-06 | 2008-05-06 | Extraction method of water-soluble spirulina polysaccharide |
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| CN101575626A true CN101575626A (en) | 2009-11-11 |
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| CNA2008100158804A Pending CN101575626A (en) | 2008-05-06 | 2008-05-06 | Extraction method of water-soluble spirulina polysaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999517A (en) * | 2010-10-28 | 2011-04-06 | 北京资源亚太饲料科技有限公司 | Preparation method and application of low-molecular weight seaweed polysaccharide trace element chelate |
| CN103947913A (en) * | 2014-05-13 | 2014-07-30 | 高天荣 | Method for reducing harmful elements in spiral seaweeds |
| CN104323065A (en) * | 2014-10-30 | 2015-02-04 | 常德炎帝生物科技有限公司 | Nostoc sphaeroids kutz jelly and preparation method |
-
2008
- 2008-05-06 CN CNA2008100158804A patent/CN101575626A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999517A (en) * | 2010-10-28 | 2011-04-06 | 北京资源亚太饲料科技有限公司 | Preparation method and application of low-molecular weight seaweed polysaccharide trace element chelate |
| CN101999517B (en) * | 2010-10-28 | 2013-11-27 | 北京资源亚太饲料科技有限公司 | Preparation method and application of low-molecular weight seaweed polysaccharide trace element chelate |
| CN103947913A (en) * | 2014-05-13 | 2014-07-30 | 高天荣 | Method for reducing harmful elements in spiral seaweeds |
| CN104323065A (en) * | 2014-10-30 | 2015-02-04 | 常德炎帝生物科技有限公司 | Nostoc sphaeroids kutz jelly and preparation method |
| CN104323065B (en) * | 2014-10-30 | 2016-04-20 | 常德炎帝生物科技有限公司 | A kind of nostoc jelly and preparation method |
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Open date: 20091111 |