CN101574430B - Chinese medicinal composition for resisting trichomonas columbae disease and method for preparing oral liquid thereof - Google Patents
Chinese medicinal composition for resisting trichomonas columbae disease and method for preparing oral liquid thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal composition for resisting trichomonas columbae disease. The Chinese medicinal composition comprises the following components by weight portion: 30 to 50 portions of houttuynia, 30 to 50 portions of fried baikal skullcap root, 25 to 40 portions of madder root, 25 to 40 portions of purple gromwell root, 15 to 30 portions of schizonepta, and 15 to 30 portions of bupleurum root. The invention also discloses a method for preparing an oral liquid of the Chinese medicinal composition. In the method, traditional Chinese medicaments are used to replace Western medicaments such as metronidazole, which are prohibited from being used for animal products, to facilitate the production of pollution-free foods. When a pigeon infects the trichomonas columbae disease, the pigeon can be better treated if taking the Chinese medicinal composition and the curative ratio is 93.33 percent, and the effectiveness ratio is 100 percent.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, the Chinese medicine composition of a kind of resisting trichomonas columbae disease of specific design and the preparation method of oral liquid thereof.
Background technology
Pluma Columba livia trichomoniasis is the modal a kind of infectiousness protozoacide of Columba livia, and its main parasitic is on the digestive tract of Columba livia, Anywhere, kind, the pigeon in the length of time all can be fallen ill, and are to support Columba livia already to threaten one of maximum commonly encountered diseases.This disease was found in the small intestinal Columba livia by Rirolta first in 1878.Show that according to foreign data and domestic investigation 80% Columba livia taeniasis is arranged approximately, the symptom of the Columba livia that grows up is not obvious, and young Columba livia and young Columba livia symptom are had outstanding performance.Young Columba livia is that disease causes thus more than 70% in its dead Columba livia in breeding process.The children Columba livia is usually infected because of tasting in the adult Columba livia crop of food " pigeon's milk " first, and keeps lifelong taeniasis.Before the immunity that forms enough protections, when infected by strong poisonous insect strain, mortality rate can be up to 50%.Even the morbidity of taeniasis squab slightly also influences alimentation, quality descends.Though medicines such as this sick available metronidazole are treated, and are prone to drug residue, cause producing be obstructed, benefit descends.
The cause of disease of Pluma Columba livia trichomoniasis is to belong to flagellum subphylum, Zoomastigophorea, polymastigina, Trichomonadidae.The fowl trichomonacide of Trichomonas (Trichmonas gallinae).Fowl trichomonacide polypide is Semen Benincasae shape or pyriform, and size is 5～9 μ m * 2～9 μ m.The polypide front end has kinetosome to stretch out 4 flagellums, and polypide can be moved rapidly.Polypide central authorities have an elongated jack-post to stretch from front to back to the polypide trailing edge.Side polypide has undulating membrane, the front end that it originates in polypide, and ending at the square after a while polypide of polypide front portion has 1 oval-shaped nuclear.Polypide is anterior relative with undulating membrane, and a side has cytostome.In Ji's nurse Sa stained preparation, the polypide protoplasm is light blue, and nucleus and kinetosome take on a red color.Flagellum is kermesinus or black, and the jack-post color is shallower than protoplasm.The fowl trichomonacide parasitizes the mucosa top layer of meat pigeon oral cavity, nasal cavity, pharynx, esophagus and crop, breeds in the mode of binary fission, and be food with mucus, mucosa fragment, microorganism, erythrocyte etc., in cytostome is taken the photograph human body, or absorb nutrition in endosmosis mode.Polypide resistance in external environment is not strong, under 20～25 ℃ of conditions, and the 3～7d of in the pathological material of disease of gathering, can surviving.Polypide is very weak to high temperature and disinfectant resistance, the 14～18d of in 4～8 ℃ of refrigerators, can surviving, in the time of 55 ℃ through 2min death; Disinfectant commonly used all can kill polypide very soon as 3～5% caustic lyes, lime cream, lysol etc.
The main route of infection of Pluma Columba livia trichomoniasis is a peroral infection.The position of Chang Jisheng of polypide and infringement is a digestive tract.Trichomonacide at first infects digestive tract, causes its ulcer, enters blood circulation from ulcer then, causes the pathological changes of organs such as liver.Can confirm this disease by the diagnostic method of cause of diseases such as direct smear microscopy, artificial culture inspection.The oral ulcer kitchen range of suffering from Columba livia is trichomonal settlement.A large amount of living worm bodies is also arranged in the saliva, and suffering from Columba livia and taeniasis Columba livia all is the source of infection.Polypide only all can infect Columba livia by drinking-water, feedstuff, wound and not closed approach such as annulus umbilicalis.Cheng Geke is by billing and cooing or polypide being directly passed to companion or the offspring of oneself by " pigeon's milk " nurture during young Columba livia.The infection of other diseases and stressors can become the inducement of primary disease.
4～14d incubation period of Pluma Columba livia trichomoniasis often infects the young Columba livia in 1～5 age in week.General 3～the 20d of the course of disease does not wait.Common aborning type mainly contains following three kinds: 1, swallows and dyspnea, lassitude, loss of appetite based on the performance of pharynx type, but the increase of drink desire, row's yellow green feces is often because of the dyspnea acute death.Cuing open on the visible oral mucosa of inspection has yellow cheesy accumulation, forms the smooth pinhead-sized focus of one deck on the mucous membrane of nasopharynx.2, random unglazed based on performance lassitude, the feather pine of visceroatonia type, row's yellow green or faint yellow mucus, pasty state feces, progressive emaciation, last depleted dead (it is reported that mortality rate can reach more than 30%).Cut open the visible internal organs of inspection the caseous necrosis kitchen range is arranged.3, performance dull mind, the appetite based on the umbilicus type reduce, become thin, the umbilical part inflammation, be reluctant to lie prostrate the very fast death of severe patient.Cut open that the visible umbilical part of inspection is subcutaneous to be had cheesy or the ulcer sexually transmitted disease (STD) becomes.
The Pluma Columba livia infusorian is to nitre azole drug sensitivity, but very easily causes in vivo residual of medicine, and carcinogenecity is arranged, and belongs to the medicine at the aquaculture forbidding.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese medicine composition of resisting trichomonas columbae disease.
The technical problem that the present invention also will solve provides the preparation method of the oral liquid of above-mentioned Chinese medicine composition.
The technical problem that the present invention also will solve provides the application of above-mentioned Chinese medicine composition in preparation resisting trichomonas columbae disease medicine.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of Chinese medicine composition of resisting trichomonas columbae disease comprises following compositions in weight portion: Herba Houttuyniae 30-50 part, Radix Scutellariae (parched) 30-50 part, Radix Rubiae 25-40 part, Radix Arnebiae (Radix Lithospermi) 25-40 part, Herba Schizonepetae 15-30 part, Radix Bupleuri 15-30 part.
The Chinese medicine composition of above-mentioned resisting trichomonas columbae disease preferably comprises following compositions in weight portion: Herba Houttuyniae 40-45 part, Radix Scutellariae (parched) 40-45 part, Radix Rubiae 30-35 part, Radix Arnebiae (Radix Lithospermi) 30-35 part, Herba Schizonepetae 20-25 part, Radix Bupleuri 20-25 part.
The Chinese medicine composition of above-mentioned resisting trichomonas columbae disease, its dosage form are oral liquid.
A kind of preparation method of oral liquid of Chinese medicine composition of resisting trichomonas columbae disease comprises the steps:
(1) Herba Houttuyniae 30-50 part, Radix Scutellariae (parched) 30-50 part, Radix Rubiae 25-40 part, Radix Arnebiae (Radix Lithospermi) 25-40 part, Herba Schizonepetae 15-30 part, Radix Bupleuri 15-30 part is mixed;
(2) in the mixed material that step (1) obtains, add 8～15 times water of mixture weight, first intense fire is heated to boiling, and slow fire boils 20～60min again, leaches medicinal liquid; The residue medicinal residues add the water of 5～10 times of amounts of medicinal residues weight and fry in shallow oil 2 times again; Merge 3 times medicinal liquid;
(3) the medicinal liquid centrifuging and taking supernatant that step (2) is obtained, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, the centrifuging and taking supernatant again;
(4) supernatant is through 100 ℃ of autoclaving 20～40min, 4 ℃ of preservations.
The application of the Chinese medicine composition of above-mentioned resisting trichomonas columbae disease in the medicine of preparation resisting trichomonas columbae disease.
Beneficial effect: the alternative Western medicine of Chinese medicinal formulae of the present invention is used for the infection of pigeon trichomonacide can reduce or eliminate the residual of Fowl meat, egg Chinese medicine, thereby helps the nuisanceless production of livestock products.
The specific embodiment:
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, process conditions and result thereof only are used to illustrate the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
Press 30 parts of prescription Herba Houttuyniae, 30 parts of Radix Scutellariae (parched), 25 parts in Radix Rubiae, 25 parts of Radix Arnebiae (Radix Lithospermi)s, 15 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription with the prepared slices of Chinese crude drugs for 15 parts, add 10 times of water gaging elder generation intense fires and be heated to boiling, slow fire boils 30min again, leaches medicinal liquid, medicinal residues add 8 times of water gagings and fry in shallow oil 2 times again, merge 3 times medicinal liquid, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, the centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations.
Embodiment 2:
With 40 parts of Herba Houttuyniae, 40 parts of Radix Scutellariae (parched), 35 parts in Radix Rubiae, 35 parts of Radix Arnebiae (Radix Lithospermi)s, 25 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription for 20 parts, adds 8 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 20min again, leaches medicinal liquid, and medicinal residues add 6 times of water gagings and fry in shallow oil 2 times again.Merge 3 times medicinal liquid, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations.
Embodiment 3:
With 50 parts of Herba Houttuyniae, 50 parts of Radix Scutellariae (parched), 40 parts in Radix Rubiae, 40 parts of Radix Arnebiae (Radix Lithospermi)s, 30 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription for 30 parts, adds 12 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 50min again, leaches medicinal liquid, and medicinal residues add 10 times of water gagings and fry in shallow oil 2 times again.Merge 3 times medicinal liquid, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 40min of each medicinal liquid, 4 ℃ of preservations.
Embodiment 4:
1, the cultivation of the preparation of Pluma Columba livia infusorian culture medium and Pluma Columba livia infusorian:
Get Columba livia liver 15g, chopping is the small rice grain size, is soaked in the 100mL distilled water, puts 4 ℃ of refrigerator overnight, and after taking out every other day, heated and boiled 30min filters through gauze, replenishes distilled water to 100mL.With peptone 2g, maltose 1g, sodium chloride 0.5g, L-cysteine hydrochloride 0.2g mixes with it, heating is short molten, filter through fine filtering paper, regulate pH to 5.6～5.8, divide the test tube of packing into, every pipe 3.5mL, 121 ℃ of autoclaving 30min, 24h in the rearmounted 37 ℃ of calorstats of cooling proves that aseptic-4 ℃ of preservations in back are standby.Carry out the wing venous blood collection with disposable needle tubing dialogue plumage Wang Ge (pigeon take from Liuhe District Donggou, Nanjing town support Columba livia field), with adopt Columba livia blood place 37 ℃ of calorstats, take out after placing 2～3h, draw upper serum with suction pipe.Columba livia serum is placed 55～56 ℃ of water-bath heating 30min deactivation, and after the packing, freezing preservation is standby.
Antibiotic selects for use penicillin 800,000 u/ to prop up, and streptomycin 1,000,000 u/ prop up each one, soak the soup dissolved dilution with the 8ml liver, preserves standby below 0 ℃.
Every pipe culture fluid 5mL, its composition are that the 3.5mL liver soaks that soup+containing antibiotic liver soaks the aseptic deactivation Columba livia of soup 0.5mL+ serum 1mL.
1 monthly age of taking from Columba livia field, Nanjing for the strain of examination worm is diagnosed as the trouble Columba livia that trichomonacide infects, and kind is white plumage Wang Ge, gets secretions with disinfecting cotton swab in oral cavity and throat portion and does the normal saline smear for microscopic examination, and trichomonacide resembles a pear in shape, and 3-4 root flagellum is arranged.After making a definite diagnosis, use for separating, cultivate trichomonacide.
Get and suffer from Columba livia oral cavity and throat portion secretions, be inoculated in the culture medium, place 37 ℃ of calorstats to cultivate, every 3d goes down to posterity once, and after 3～4 generations, therefrom sampling is transferred in the culture fluid of other one bottle of 20ml then through in-vitro cultivation, treat to be used for this experiment after its a large amount of breedings.
The Pluma Columba livia infusorian is cultivated, observed result: the Pluma Columba livia infusorian moves active in culture medium, its morphosis is all near the naturalness of normal saline smear, polypide resembles a pear in shape, ellipse or circular, size is 6～10um * 2～9um, the body front end has 4 tractellums, wherein a flagellum turns back to the body rear end and invests on the undulating membrane, and end has the axle thorn like the tail sample, makes corkscrew motion in liquid.
Liver is soaked soup cultivation trichomonacide and increases sharply since 1d quantity, and 3d reaches summit of growth, and polypide quantity descends gradually behind 4d, generally most polypide death when 5d.External when carrying out blind passage, the 3rd～4 generation polypide the most stable, polypide death when reaching for the 6th generation.The 3rd day in the polypide breeding begins counting, and after culture tube was shaken up, aseptic condition was counted with blood cell counting plate with suction pipe sucking-off 20ul down.Borer population alive in this experiment culture fluid is about 2 * 10
5Individual/ml.
2, Chinese medicine composition trichomonacide effect measuring of the present invention:
(1) single medicinal material is to Pluma Columba livia infusorian inhibition test
17 kinds of Chinese medicines are all bought in Nanjing state and are contained chain pharmacy, are respectively Radix Bupleuri, Flos Buddlejae, Radix Rubiae, Herba Schizonepetae, snakeweed, Radix Scutellariae (parched), Herba Solidaginis, Gu Jingcao, Herba Agrimoniae, Radix Cynanchi Paniculati, Herba Artemisiae Anomalae, Herba Pyrolae, Rhizoma Cimicifugae, Herba Solani Lyrati, Cacumen Securinegae Suffruticosae, Herba Houttuyniae, Radix Arnebiae (Radix Lithospermi).17 kinds of Chinese herbal medicine are boiled respectively, the concrete grammar that boils is: with 100mL water logging bubble Chinese medicine 30min, carry out decocting then, decocting 1h after-filtration medicinal residues are got medicinal liquid one time, to remain medicinal residues again and add water and boil, the 1h after-filtration is got the secondary medicinal liquid, after twice medicinal liquid mixed, heating is condensed into 10ml, places 4 ℃ of refrigerators standby.
Get the above-mentioned Pluma Columba livia infusorian culture fluid of having cultivated 3 days, shake up gently, getting 1mL respectively joins in 18 culture tubes, preceding 17 pipes adding respectively are subjected to the reagent thing, last 1 pipe is control tube, and behind the adding medicine, each culture tube is put and cultivated in 37 ℃ of calorstats, 2h, 6h calculate each pipe borer population alive respectively after dosing, and compare with the borer population alive that does not add the medicine culture tube.
The result: in 6 hours pest-resistant viewing tests of 17 flavor Chinese medicines, having 13 flavors to have pest-resistant effect, have 4 flavors not have pest-resistant effect, is respectively Flos Buddlejae, Gu Jingcao, Herba Agrimoniae, Herba Agrimoniae and Radix Cynanchi Paniculati; Snakeweed, Rhizoma Cimicifugae, Herba Solani Lyrati still can not kill trichomonacide fully within 6 hours; All the other medicines all can kill trichomonacide fully in 6 hours, the result sees table 1 for details.
The drug sensitive experiment result of 17 kinds of common Chinese medicine killing in vitro Pluma Columba livia infusorian of table 1
Data according to table 1, Radix Bupleuri, Radix Rubiae, Herba Schizonepetae, Radix Scutellariae (parched), Herba Solidaginis, Radix Arnebiae (Radix Lithospermi) have the effect of significantly killing the Pluma Columba livia infusorian as can be seen, behind dosing 2h, the disintegrate death of most polypides, still there is discrete polypide that very weak mobility is arranged, but flagellar movement power is lost, behind dosing 6h, and the whole dead disintegrates of polypide.Herba Houttuyniae, snakeweed etc. have certain effect to the Pluma Columba livia infusorian, and action effect becomes along with the prolongation of time significantly, and behind dosing 2h, the motor capacity of polypide weakens, and flagellum is LOM almost, part polypide disintegrate death.Behind dosing 6h, the whole disintegrate death of polypide.And the polypide of matched group, in the time period of 2h～6h, polypide is movable normal, and flagellum has very strong motor capacity, and form is normal.Comprehensive above-mentioned experimental result is screened 17 kinds of Chinese medicines, forms Chinese medicine compound.
(2) herbal mixture of the present invention is to Pluma Columba livia infusorian inhibition test:
With 50 parts of Herba Houttuyniae, 50 parts of Radix Scutellariae (parched), 40 parts in Radix Rubiae, 40 parts of Radix Arnebiae (Radix Lithospermi)s, 30 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription for 30 parts, adds 10 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 30min again, leaches medicinal liquid, and medicinal residues add 8 times of water gagings and fry in shallow oil 2 times again.Merge 3 times medicinal liquid, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations.
The Chinese medicine oral liquid of extracting is diluted to every milliliter with sterile saline contains crude drug 0.5g/mL and 1.0g/mL.Respectively get 1mL and add test tube as the 1st pipe and the 2nd pipe, other adds the 1mL sterile saline in the 3rd pipe in contrast.The trichomonacide culture fluid is shaken up gently, draw 1mL respectively and join in 3 culture tubes, make the drug level of the 1st pipe and the 2nd pipe be respectively 0.25g/mL and 0.5g/mL.Each culture tube is put in 37 ℃ of calorstats and is cultivated, and per half an hour, jog was 1 time.2h, 4h, the 6h worm that observe to live of taking a sample respectively after dosing is calculated each pipe borer population of living, and compares with the borer population alive of the culture tube that does not add medicine.
The result: this Chinese medicine oral liquid has the effect of tangible anti-Pluma Columba livia infusorian, and along with the growth with action time of increasing of drug level, kills trichomonal effect and significantly strengthen.For the test group of 0.25g/mL, behind dosing 2h, the polypide activity is more active, and flagellar movement power is stronger, the polypide well-grown, behind dosing 4h, the polypide hypoergia, the part distortion is expanded, most polypides lose mobility, behind dosing 6h, and the whole disintegrates of polypide; For the test group of 0.5g/mL, behind dosing 2h, the just disintegrate death of most polypides only has indivedual polypides extremely weak movable, behind dosing 4h, and the whole disintegrates of polypide.And the polypide of matched group morphosis in the time period of 2h～6h is normal, and behavior is active, and flagellar movement is normal.
Compound Chinese medicinal preparation is killed trichomonal effect and is seen Table 2.
The drug sensitive experiment result of table 2 Chinese medicinal formulae killing in vitro Pluma Columba livia infusorian
Embodiment 5: different dose combinations are to Pluma Columba livia infusorian inhibition test.
With 30 parts of Herba Houttuyniae, 30 parts of Radix Scutellariae (parched), 25 parts in Radix Rubiae, 25 parts of Radix Arnebiae (Radix Lithospermi)s, 15 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription with the prepared slices of Chinese crude drugs for 15 parts, adds 10 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 30min again, leaches medicinal liquid, and medicinal residues add 8 times of water gagings and fry in shallow oil 2 times again.Merge 3 times filtrate, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 1g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations get prescription I.
With 40 parts of Herba Houttuyniae, 40 parts of Radix Scutellariae (parched), 35 parts in Radix Rubiae, 35 parts of Radix Arnebiae (Radix Lithospermi)s, 25 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription for 20 parts, adds 10 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 30min again, leaches medicinal liquid, and medicinal residues add 8 times of water gagings and fry in shallow oil 2 times again.Merge 3 times filtrate, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 1g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations get prescription II.
With 50 parts of Herba Houttuyniae, 50 parts of Radix Scutellariae (parched), 40 parts in Radix Rubiae, 40 parts of Radix Arnebiae (Radix Lithospermi)s, 30 parts of Herba Schizonepetae, Radix Bupleuri is made into prescription for 30 parts, adds 10 times of water gaging elder generation intense fires and is heated to boiling, and slow fire boils 30min again, leaches medicinal liquid, and medicinal residues add 8 times of water gagings and fry in shallow oil 2 times again.Merge 3 times filtrate, centrifugal, slow fire boiling is concentrated into every milliliter and contains crude drug 1g, centrifuging and taking supernatant, packing.The equal 100 ℃ of autoclaving 30min of each medicinal liquid, 4 ℃ of preservations get prescription III.
The Chinese medicine concentrated solution of above-mentioned 3 prescriptions is respectively got 1mL, 3 test tubes of packing, other adds the 1mL sterile saline in the 4th pipe in contrast.The trichomonacide culture fluid of cultivating is shaken up gently, draw 1mL respectively and join in 4 culture tubes, shake up, each culture tube is put in 37 ℃ of calorstats and is cultivated, and per half an hour, jog was 1 time.2h, 4h, the 6h worm that observe to live of taking a sample respectively after dosing is calculated each pipe borer population of living, and compares with the borer population alive of the culture tube that does not add medicine.
Result: do not see the death that except that adding saline tube the 2nd to the 3rd pipe is all death of trichomonacide after two hours, shows that the prescription of above-mentioned various combination all can suppress the growth of Pluma Columba livia infusorian effectively.The results are shown in Table 3.
The drug sensitive experiment result of the different dose Chinese medicinal formulae of table 3 killing in vitro Pluma Columba livia infusorian
Embodiment 6:
According to prescription and the method for embodiment 2, the Chinese medicine oral liquid of preparing q.s is stand-by.
Select white plumage Wang Ge of the 1 monthly age meat pigeon of 60 health,, make its infection (being as the criterion to trichomonacide), they are divided into four groups then, 15 every group with oral cavity cotton swab sampling microscopy with the inoculation of isolating Pluma Columba livia infusorian oral cavity.The 1st group, the 2nd group and be suspended in the clean water with the Chinese medicine oral liquid that contains crude drug amount 0.5g, 1g and 1.5g respectively the 3rd group every every day and drink, serve on 10 days for Columba livia.The 4th group is matched group, not administration.Each organizes isolated rearing, observes the Columba livia oral cavity respectively at the 5th day and the 10th day and has or not the yellow-white exudate, does the buccalis microscopy simultaneously.All oral cavities are seen yellow-white and are oozed out point, and microscopy go out to have trichomonal classify as invalid; The oral cavity does not see that yellow-white oozes out point, and microscopy does not see that trichomonal healing of classifying as is arranged; The yellow-white exudate is not seen in the oral cavity but microscopy goes out to have trichomonal, and perhaps healing person classifies as effectively; Add up effective percentage, cure rate (table 4) respectively.
Table 4 various dose Chinese medicine oral liquid is cured test to artificial Pluma Columba livia trichomonacide
Annotate: invalid: the oral cavity is seen yellow-white and is oozed out point, and microscopy goes out to have trichomonacide; Cure: the oral cavity does not see that yellow-white oozes out point, and microscopy does not see that trichomonal healing of classifying as is arranged; Effectively: the yellow-white exudate is not seen in the oral cavity but microscopy goes out to have trichomonal, and healing person also classifies as effectively.
Through observe, statistics, the treatment group that every Columba livia dosage every day is 1g was through 10 days treatment, sick Pluma Columba livia trichomonacide treatment effective percentage 100% is cured 13, cure rate 86.67%; The treatment group curative effect that every Columba livia dosage every day is 1.5g is best, and sick Pluma Columba livia trichomonacide treatment effective percentage 100% is cured 14, cure rate 93.33%.Every every day dosage be the 1st group of 0.5g treatment effective percentage 80%, cure 11, cure rate 73.33%.Untreated matched group does not have spontaneous recovery.The result shows, reach satisfactory effect, and therapeutic dose can be by every Columba livia 1g crude drug content suspendible drink-service every day, and need reach more than 10 days the course of treatment.
Claims (5)
1. the Chinese medicine composition of a resisting trichomonas columbae disease is characterized in that said composition made by the component of following parts by weight: Herba Houttuyniae 30-50 part, Radix Scutellariae (parched) 30-50 part, Radix Rubiae 25-40 part, Radix Arnebiae (Radix Lithospermi) 25-40 part, Herba Schizonepetae 15-30 part, Radix Bupleuri 15-30 part.
2. the Chinese medicine composition of resisting trichomonas columbae disease according to claim 1 is characterized in that said composition made by the component of following parts by weight: Herba Houttuyniae 40-45 part, Radix Scutellariae (parched) 40-45 part, Radix Rubiae 30-35 part, Radix Arnebiae (Radix Lithospermi) 30-35 part, Herba Schizonepetae 20-25 part, Radix Bupleuri 20-25 part.
3. the Chinese medicine composition of resisting trichomonas columbae disease according to claim 1 and 2, the dosage form that it is characterized in that this Chinese medicine composition is an oral liquid.
4. the preparation method of the oral liquid of the Chinese medicine composition of a resisting trichomonas columbae disease is characterized in that this method comprises the steps:
(1) Herba Houttuyniae 30-50 part, Radix Scutellariae (parched) 30-50 part, Radix Rubiae 25-40 part, Radix Arnebiae (Radix Lithospermi) 25-40 part, Herba Schizonepetae 15-30 part, Radix Bupleuri 15-30 part is mixed;
(2) in the mixed material that step (1) obtains, add 8～15 times water of mixture weight, first intense fire is heated to boiling, and slow fire boils 20～60min again, leaches medicinal liquid; The residue medicinal residues add the water of 5～10 times of amounts of medicinal residues weight and fry in shallow oil 2 times again; Merge 3 times medicinal liquid;
(3) the medicinal liquid centrifuging and taking supernatant that step (2) is obtained, slow fire boiling is concentrated into every milliliter and contains crude drug 2g, the centrifuging and taking supernatant again;
(4) supernatant is through 100 ℃ of autoclaving 20～40min, 4 ℃ of preservations.
5. the application of the Chinese medicine composition of the described resisting trichomonas columbae disease of claim 1 in the medicine of preparation resisting trichomonas columbae disease.
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| CN103735965B (en) * | 2013-12-30 | 2016-05-18 | 华南农业大学 | A kind of Chinese medicine compound prescription of preventing and treating Trichomonad of dove and preparation method thereof and application |
| CN108635492A (en) * | 2018-07-10 | 2018-10-12 | 广东科贸职业学院 | A kind of Chinese medicine composition and the preparation method and application thereof of prevention Trichomonad of dove |
| CN114657093B (en) * | 2022-03-07 | 2023-07-07 | 北京市农林科学院 | Pigeon-derived lactobacillus crispatus BL4014 and application thereof |
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| CN101214311A (en) * | 2007-12-27 | 2008-07-09 | 四川兴华药业有限责任公司 | Compound scutellaria injection for treating fowls and domestic animals infectious disease and preparation thereof |
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| CN101214311A (en) * | 2007-12-27 | 2008-07-09 | 四川兴华药业有限责任公司 | Compound scutellaria injection for treating fowls and domestic animals infectious disease and preparation thereof |
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