CN101530163A - Method for keeping activity of probiotic - Google Patents
Method for keeping activity of probiotic Download PDFInfo
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- CN101530163A CN101530163A CN200910031028A CN200910031028A CN101530163A CN 101530163 A CN101530163 A CN 101530163A CN 200910031028 A CN200910031028 A CN 200910031028A CN 200910031028 A CN200910031028 A CN 200910031028A CN 101530163 A CN101530163 A CN 101530163A
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Abstract
The invention relates to a method for keeping activity of probiotic. The probiotic is adsorbed into holes of microporous starch to generate a microporous adsorbate, and the microporous adsorbate and a mixture of nutrients required by aquatic seedlings form a core material; chitosan is used as a wall material, wherein the chitosan is insoluble in water but soluble in 1 percent lactic acid solution; and a microcapsule is prepared by using a spraying and drying method, the grain diameter of the microcapsule is less than 150mu m, and the ratio of the wall material to the core material is (3-10):100. The method can prevent the inactivation of the probiotic, and can play a role in slow release. The aperture of the micropore is 1mu m; the microporous starch, the ratio of the micropore cavity volume of which to the total volume of the starch is more than 50 percent, is used for adsorbing the probiotic into the holes of the microporous starch; the adsorbate of the microporous starch, which adsorbs the probiotic, is used as the core material; and the chitosan is used as a coating of the wall material. The method has the advantages that the microcapsule prepared by micropore adsorption and envelope can effectively keep the activity of the envelope during processing and manufacturing through experiment so as to develop the application prospect of the probiotic in aquatic feed.
Description
Technical field:
What the present invention relates to is a kind of method that keeps activity of probiotic.Belong to feed and field of feed additive technology.
Background technology:
Microorganism formulation is active bacteria formulation (Biogen), bacteria-promoting agent, probiotic (Probitics) again, it is under little ecological theory instructs, by Bacillus acidi lactici, bacillus. beneficial microbes such as saccharomycete (PM), biologic product or the active bacteria formulation produced through special process such as compound cultivation, fermentation, drying, processing, it can replenish the normal microorganism that lacks or lack in the animal intestinal on quantity or kind, adjust or keep microecological balance in the enteron aisle, improve and strengthen the immunity function of body, improve the anti-stress ability of body.The mechanism of action of microbial product mainly shows physiological metabolism characteristics and microbial growth modes of reproduction two aspects of microorganism.By the eubolism of profitable strain, produce the pH value that lactic acid, acetate, propionic acid etc. reduce organismic internal environment; Produce hydrogen peroxide and kill some potential pathogens; Producing some metabolites descends the concentration of interior ammonia of enteron aisle and amine; Produce proferment and promote substance decomposition; Synthetic B family vitamin, generation antibacterial material; Produce the nospecific immunity regulatory factor, improve the animal's antibody level and hugely have a liking for bacterial activity, its effect prerequisite is that beneficial bacterium becomes the dominant bacteria in the system.Simultaneously microorganism take oxygen by force and barrier action also is its mode of action.Microbial product is exactly the quantity of former flora in the artificial additional animal body in fact, strengthens its vigor.The antibiotic of feeding for a long time makes the bacterium that develops immunity to drugs in the animal body, even causes the interior antibiotic high concentration of animal body residual, and antibiotic-resistant bacteria and high residue antibiotic are per mu harmful to going into.Microbial product does not have residue problem, can also improve efficiency of feed utilization 17%～23%.
Therefore adding the quantity that probio is replenished former flora in the animal body, strengthen its vigor, is necessary and significant.Yet microbial product after being heated, most of probio devitalization and lose effect.This makes aquatic micro-capsule bait can't use the microbial product of thermal sensitivity in the processing pelletization.Therefore, keep the compound probiotic vigor, solving the probio thermal stability problems is exactly the problem of the required solution of present feed sector application probio.
Summary of the invention:
The present invention proposes a kind of process that keeps activity of probiotic, is intended to overcome the above-mentioned defective of existing in prior technology, to solve the possibility that probio survives in hot and humid environment.
Technical solution of the present invention: the technology that it is characterized in that this method: probio is adsorbed in the hole of micropore starch, adding each required nutrients of aquatic seedling (micro mist particle degree is less than 0.046 μ m) in addition with above-mentioned micro-porous adsorption thing is core, and the weakly acidic aqueous solution that is dissolved in 1% lactic acid solution with shitosan is made wall material dressing; Make microcapsules with spray drying process.During microcapsules, the ratio of wall material and core is (3-10) in preparation: 100, preventing the inactivation of probio, and can play slow releasing function, and improved heat endurance.
Described micropore size is at 1 μ m, and the ratio of micropore cavity volume and starch cumulative volume is adsorbed in used probio in the aperture of micropore starch greater than 50% micropore starch.
The described weakly acidic aqueous solution that is dissolved in 1% lactic acid solution with shitosan is made the wall material.
Described method is a spray drying process, and turnover wind-warm syndrome degree is respectively 200 ℃ and 70 ℃, and dry back moisture content is controlled at 6 ± 0.8%.
Microporosity starch is meant the starch with many apertures.Therefore microporosity starch surface and inner owing to the structure with cellular porous property has the good adsorption performance, is a kind of new bio sorbing material.The micropore size of microporosity starch is about 1 μ m.The vestibule volume of microporosity starch and the ratio of starch cumulative volume are greater than 50%.
Beneficial effect of the present invention: the effect of adding probio in the feed is more and more affirmed and application.1, improves the inside and outside ecological environment, decompose and reduced carcinogen lactic acid bacteria growth and breeding in enteron aisle and can produce materials such as organic acid, bacteriocin, reduce the activity of urase, improve the utilization rate of protein,, reduce the ammonia amount of discharging with fecaluria, improve environmental condition.2, strengthen immunologic function, resist the infection probiotics and guaranteed the normal operation of gene flow, energy stream and material stream in the microecosystem, growth of animals or poultry is accelerated, promote the improvement of meat, fat, cholesterol level in the meat are obviously descended.3, the paired foreign nationality's harmful microbe of mutual group chemical barriers such as biological antagonist invasive organism beneficial microorganism metabolite such as acetate, propionic acid, lactic acid, antibiotic and bacteriocin; The ancestral home flora is colonizated in surfaces such as mucous membrane, skin orderlyly or forms biological barrier between cell.These barriers can stop the field planting of pathogenic microorganism, play occupy-place, contention nutrition, mutualism or short of moneyly draw effect.4, biology is taken oxygen, dominant population and competitive exclusion aerobic bacillus by force and is entered animal gastrointestinal tract consume oxygen in the growth and breeding process, form anaerobic environment, help anaerobic bacteria (as Bacillus acidi lactici, Bifidobacterium) growth, recover microecological balance, or the recovery dominant population, make unbalance little ecology reach new balance.And we use antibiotic, when eliminating pathogenic bacteria, have also eliminated useful physiological bacterium, have destroyed the ecological balance of enteric microorganism, cause the neurological susceptibility of animal to pathogenic microorganism.5, produce multiple enzyme, improve food conversion ratio.6, promote animal physiological function test of maturity to prove that little ecological reagent can increase the absorption area of small intestine, quicken the utilization of animal body, improve the metaboilic level of polysaccharide, and can promote the synthetic of RNA, DNA and protein glycogen.Probiotics does not have residue problem, can also improve efficiency of feed utilization 17%～23%.
Yet the application of probio has a lot of restrictive conditions in the feed, as: temperature, humidity, acid-base value, etc. (particularly temperature), can make the probio inactivation and lose meaning.In micro-capsule bait granulation dry run, need long heat drying.Under this condition, keep the activity of probio just to become the problem of being badly in need of solution.Evidence the present invention makes microcapsules by micro-porous adsorption and coating can effectively keep the activity of probio in granulation.Thereby opened up probio application prospect extensively in aquatic feeds.
Description of drawings
Accompanying drawing 1 is technological process of production figure of the present invention.
The specific embodiment
Embodiment 1 (is example with the Bacillus acidi lactici)
Every gram cornstarch prepares micropore starch (preparation method's prior art) with 100 microgram a-amylase, reaction time is 12 hours, reaction temperature be 55 ℃, the reaction PH be 6.0, (the micropore size is about 1 μ m to prepare preferable micropore starch with this understanding.The vestibule volume of microporosity starch and the ratio of starch cumulative volume are greater than 50%), adsorb (also claiming embedding) 50g lactic acid bacteria in its vestibule with this micropore starch 100g.
Adding each required nutrients of aquatic seedling (micro mist particle degree is less than 0.046 μ m) gross weight 100kg in addition with above-mentioned thing again is core, being dissolved in 400ml lactic acid with the 1kg shitosan adds the 40kg aqueous solution and makes the wall material, make microcapsules. the most frequently used method of system microcapsules is a spray drying process, turnover wind-warm syndrome degree is respectively 200 ℃ and 70 ℃, and dry back moisture content is controlled at 6 ± 0.8%.
Embodiment 2 (is example with the Bacillus acidi lactici)
Every gram cornstarch prepares micropore starch (using prior art) with 100 microgram a-amylase, the reaction time for the 12H reaction temperature be 55 ℃, the reaction PH be 6.0, (the micropore size is about 1 μ m to prepare preferable micropore starch with this understanding.The vestibule volume of microporosity starch and the ratio of starch cumulative volume are greater than 50%), adsorb (also claiming embedding) 55g Bacillus acidi lactici in its vestibule with this micropore starch 100g.
Adding each required nutrients of aquatic seedling (micro mist particle degree is less than 0.046 μ m) gross weight 100kg in addition with above-mentioned thing again is core, being dissolved in 400ml lactic acid with the 1kg shitosan adds the 40kg aqueous solution and makes the wall material, make microcapsules. the most frequently used method of system microcapsules is a spray drying process, turnover wind-warm syndrome degree is respectively 200 ℃ and 70 ℃, and dry back moisture content is controlled at 6 ± 0.8%.
Embodiment 3 (is example with the Bacillus acidi lactici)
Every gram cornstarch prepares micropore starch (using prior art) with 100 microgram a-amylase, the reaction time for the 12H reaction temperature be 55 ℃, the reaction PH be 6.0, (the micropore size is about 1 μ m to prepare preferable micropore starch with this understanding.The vestibule volume of microporosity starch and the ratio of starch cumulative volume are greater than 50%), adsorb (also claiming embedding) 60g Bacillus acidi lactici in its vestibule with this micropore starch 100g.
Adding each required nutrients of aquatic seedling (micro mist particle degree is less than 0.046 μ m) gross weight 100kg in addition with above-mentioned thing again is core, being dissolved in 400ml lactic acid with the 1kg shitosan adds the 40kg aqueous solution and makes the wall material, make microcapsules. the most frequently used method of system microcapsules is a spray drying process, turnover wind-warm syndrome degree is respectively 200 ℃ and 70 ℃, and dry back moisture content is controlled at 6 ± 0.8%.
Attached; Other adds each required nutrients of aquatic seedling, and is as follows:
Imported fish meal 50kg, fermentation bean powder 18kg, the pure liver cream of cuttlefish 4kg, alphalise starch 20kg
Fish oil 1kg, phosphatidase 11 kg, additive (containing vitamin and mineral element) 6kg.
Claims (5)
1, a kind of method that keeps activity of probiotic is characterized in that this method comprises following processing step:
One, probio is adsorbed in the hole of micropore starch, thing with each required nutrients mixing of micro-porous adsorption thing and aquatic seedling is a core, its micro mist particle degree is a core less than 0.046 μ m, and the weakly acidic aqueous solution that is dissolved in 1% lactic acid solution with shitosan is a wall material dressing; Two, make microcapsules with spray drying process; Three, in preparation during microcapsules, the ratio of wall material dressing and core is (3-10): 100, preventing the inactivation of probio, and can play slow releasing function, and improved heat endurance.
2, a kind of method that keeps activity of probiotic according to claim 1, the aperture that it is characterized in that described micropore starch is at 1 μ m, the ratio of micropore cavity volume and starch cumulative volume is adsorbed in used probio in the aperture of micropore starch greater than 50% micropore starch.
3, according to the described a kind of method that keeps activity of probiotic of claim 1, it is characterized in that described to add each required nutrients weight ratio of aquatic seedling in addition with the micro-porous adsorption thing as follows: imported fish meal accounts for 50%, the fermentation bean powder accounts for 18%, the pure liver cream of cuttlefish accounts for 4%, alphalise starch accounts for 20%, fish oil accounts for 1%, and phosphatidase 11 kg accounts for 1%, and additive accounts for 6%.
4, a kind of method that keeps activity of probiotic according to claim 2 is characterized in that described micropore starch, and the reaction time in its preparation process is 12 hours, reaction temperature be 55 ℃, the reaction PH be 6.0.
5, a kind of method that keeps activity of probiotic according to claim 2 is characterized in that the described spray drying process of using, and turnover wind-warm syndrome degree is respectively 200 ℃ and 70 ℃, and dry back moisture content is controlled at 6 ± 0.8%.
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| CN2009100310280A CN101530163B (en) | 2009-04-24 | 2009-04-24 | Method for keeping activity of probiotic |
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| CN2009100310280A CN101530163B (en) | 2009-04-24 | 2009-04-24 | Method for keeping activity of probiotic |
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| CN101530163B CN101530163B (en) | 2011-08-24 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198115A (en) * | 2011-05-19 | 2011-09-28 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
| CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
| CN104472876A (en) * | 2014-12-10 | 2015-04-01 | 张家界恒兴生物科技有限公司 | Production method of special plant feed additive for aquaculture |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
| CN116898983A (en) * | 2023-07-18 | 2023-10-20 | 安徽善和生物科技有限公司 | High-stability crystal ball probiotics and application thereof |
-
2009
- 2009-04-24 CN CN2009100310280A patent/CN101530163B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198115A (en) * | 2011-05-19 | 2011-09-28 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
| CN102198115B (en) * | 2011-05-19 | 2012-11-14 | 甘肃易源生物制药有限公司 | Enteric-coated micro-capsule prepared by mixing traditional Chinese medicine extract and probitics |
| CN102919542A (en) * | 2012-11-13 | 2013-02-13 | 西华大学 | Multistage-controlled-release probiotics microecological preparation composite feed for Silurus meridionalis and preparation method thereof |
| CN104472876A (en) * | 2014-12-10 | 2015-04-01 | 张家界恒兴生物科技有限公司 | Production method of special plant feed additive for aquaculture |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
| CN116898983A (en) * | 2023-07-18 | 2023-10-20 | 安徽善和生物科技有限公司 | High-stability crystal ball probiotics and application thereof |
| CN116898983B (en) * | 2023-07-18 | 2024-03-26 | 安徽善和生物科技有限公司 | High-stability crystal ball probiotics and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101530163B (en) | 2011-08-24 |
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