CN101524132A - Carrot pectin and preparation method and application thereof - Google Patents
Carrot pectin and preparation method and application thereof Download PDFInfo
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- CN101524132A CN101524132A CN200910029387A CN200910029387A CN101524132A CN 101524132 A CN101524132 A CN 101524132A CN 200910029387 A CN200910029387 A CN 200910029387A CN 200910029387 A CN200910029387 A CN 200910029387A CN 101524132 A CN101524132 A CN 101524132A
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Abstract
The invention discloses carrot pectin and a preparation method and the application thereof. The method comprises the following steps: adding 3 to 15 times amount of ammonium oxalate water solution into carrots or industrial waste residue of carrots after the extraction of carotene and stirring at 30 to 100 DEG C for 0.5 to 3 hours; filtering and concentrating the mixture; adding 1 to 6 times amount of alcohol into the mixture; separating solid from liquid; and taking out and drying sediment to obtain the carrot pectin. Pharmacological experimental researches show that, the carrot pectin provided by the invention has the function of removing heavy metal, does not have poisonous side effects, has clear component, controllable quality and simple preparation method and process, can be developed into health-care food, food additive or drug. The preparation method extracts pectin from the industrial waste residue in carotene production, thereby having low cost, being suitable for industrial production, turning waste into valuables and reasonably utilizing resources.
Description
Technical field
The present invention relates to a kind of plant extracts, specifically relate to a kind of carrot pectin and its preparation method and the application in preparation row's heavy metal health food or row's heavy metal medicine thereof.
Background technology
Along with society, industrial development, the vehicle exhaust that contacts in people's daily life, newspaper, paint of coating, porcelain bowl dish, old and useless battery even edible vegetables, drinking water, milk powder, instant noodles, dry fruit, jam product, all there is serious heavy metals exceeding standard problem in dilated foods etc., common heavy metal mainly comprises mercury, copper, plumbous, zinc, iron, cobalt, nickel, manganese, cadmium, tungsten, molybdenum, gold etc., the human body heavy metals exceeding standard can cause people's headache, dizzy, insomnia, forgetful, refreshing smart entanglement, arthralgia, calculus, even can cause cancer as liver cancer, cancer of the stomach, intestinal cancer, breast cancer or prostate cancer etc., human body heavy metals exceeding standard especially can produce serious toxic and side effect to people's digestive system and urinary system.
In these several toxic heavy metals, plumbous harm maximum to human body, next is arsenic and mercury, child's brain is more responsive to lead contamination, can have a strong impact on children's intelligence development and behavior, when plumbous content surpasses 0.6 μ g/mL in children's blood, intellectual developmental disorder and abnormal behavior will appear, according to statistics, in nearly 300,000,000 children of China, there are 1.76 hundred million children blood leads to exceed standard.
Many for the treatment of heavy metal of body content overproof at present based on chemicals, stronger as calcium calcium trisodium pentetate and the effect of natrium adetate row heavy metal, but stronger renal toxicity is arranged, both are the wide spectrum complexing agent, can discharge the indispensable trace element of a large amount of human bodies during medication, and can not oral application; Penicillamine also has the effect of row's heavy metal, can be oral, but effect is relatively poor, and cause allergy easily.
Research to carrot at present is mainly carrotene, to carrot after extracting carrotene, all abandon as industrial residue, the carrot pectin that also contains 8-10% weight in the carrot, extracting method, structure and activity research to the more pectin composition of content in the carrot in the prior art do not appear in the newspapers.
Summary of the invention
Goal of the invention: the objective of the invention is to overcome the deficiencies in the prior art, provide a kind of arrange the heavy metal effect by force, safely, the carrot pectin that has no side effect and preparation method thereof, a further object of the invention provides the application of carrot pectin in preparation row's heavy metal health food or row's heavy metal medicine.
Technical scheme: for realizing above purpose, carrot pectin provided by the invention prepares by the following method: get the ammonium oxalate aqueous solution that dried carrot or carrot industrial residue add 3 to 15 times of amounts, at 30 to 100 ℃ of following constant temperature stirring reactions 0.5 to 3 hour, filtration, concentrate, add alcohol, Separation of Solid and Liquid, the taking precipitate of 1 to 6 times of amount, be drying to obtain carrot pectin.
The method for preparing carrot pectin provided by the invention is: get the ammonium oxalate aqueous solution that dried carrot or carrot industrial residue add 3 to 15 times of amounts, at 30 to 100 ℃ of following constant temperature stirring reactions 0.5 to 3 hour, filtration, concentrate, add alcohol, Separation of Solid and Liquid, the taking precipitate of 1 to 6 times of amount, be drying to obtain carrot pectin.
As ammonium formiate concentration used among the above-mentioned preparation method of preferred version is 0.3% to 5%, and the consumption of ammonium oxalate is 15 times of the carrot quantity of slag.
As reaction temperature among the above-mentioned preparation method of preferred version is 90 ℃, and the reaction time is 1.5 hours.
As used alcohol among the above-mentioned preparation method of preferred version is ethanol, and consumption of ethanol is 2 times of the concentrate amount.
The carrot pectin that the present invention's invention provides can be used for preparation row heavy metal health food.
Carrot pectin provided by the invention also can become jelly, jam or fruit juice etc. with edible preparing carriers.
Carrot pectin provided by the invention also can become food additives with edible preparing carriers.
Carrot pectin provided by the invention can be used for preparation row heavy metal medicine.
Carrot pectin energy provided by the invention and pharmaceutically acceptable carrier are made the medicine of tablet, capsule, granule, micro-capsule, ointment formulation.
Be to realize the application of carrot pectin, add lactose and starch when making tablet or capsule, add magnesium stearate lubricant in case of necessity at preparation row heavy metal medicine, whole grain, dry, make granule or compressing tablet is made tablet or the encapsulated capsule of making; Or the dress micro-capsule is made microcapsules; Add tween, beeswax or polyethylene glycol when making ointment, mixing is made ointment;
The present invention's advantage compared with prior art has:
1, carrot pectin is to extract from people vegetables carrot commonly used, and carrot pectin is developed to health food or the medicine with the effect of row's heavy metal, the advantage that have safely, has no side effect.
2, extract pectin from the industrial waste of producing carrotene, cost is low, is fit to suitability for industrialized production, and can turns waste into wealth, and makes rational use of resources, and meets current sustainable development view.
3, to have technology workable for the method for preparing carrot pectin provided by the invention, and cost is low, and carrot pectin recovery rate height is fit to suitability for industrialized production.
4, carrot pectin provided by the invention can be prepared into health food with edible carrier, also can become the medicine of different dosage form with preparing carriers pharmaceutically.
5, it mainly forms by β-glycosidic bond with galacturonic acid carrot pectin provided by the invention through structural research, and chemical constitution is clear, steady quality, controlled.
The specific embodiment:
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, process conditions and result thereof only are used to illustrate the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
Get 20 kilograms of carrot industrial residues, dry, 15 times of amounts of adding concentration are 0.5% the ammonium oxalate aqueous solution, at 90 ℃ of following constant temperature stirring reactions 1.5 hours, filtration, concentrate, add ethanol, Separation of Solid and Liquid, the taking precipitate of 2 times of amounts, be drying to obtain 2.19 kilograms of carrot pectins.(lot number 01)
Embodiment 2:
Get 20 kilograms of carrot industrial residues, dry, 3 times of amounts of adding concentration are 0.3% the ammonium oxalate aqueous solution, at 30 ℃ of following constant temperature stirring reactions 0.5 hour, filtration, concentrate, add ethanol, Separation of Solid and Liquid, the taking precipitate of 1 times of amount, be drying to obtain 1.53 kilograms of carrot pectins.(lot number 02)
Embodiment 3:
Get 20 kilograms of carrot industrial residues, dry, 8 times of amounts of adding concentration are 2% the ammonium oxalate aqueous solution, at 60 ℃ of following constant temperature stirring reactions 2 hours, filtration, concentrate, add ethanol, Separation of Solid and Liquid, the taking precipitate of 4 times of amounts, be drying to obtain 1.99 kilograms of carrot pectins.(lot number 03)
Embodiment 4:
Get 20 kilograms of carrot industrial residues, dry, 15 times of amounts of adding concentration are 5% the ammonium oxalate aqueous solution, at 100 ℃ of following constant temperature stirring reactions 3 hours, filtration, concentrate, add ethanol, Separation of Solid and Liquid, the taking precipitate of 6 times of amounts, be drying to obtain 2.21 kilograms of carrot pectins.(lot number 04)
Embodiment 5: carrot pectin lead discharging pharmacological evaluation
1, be subjected to the reagent thing:
Carrot pectin, prepare voluntarily (lot number: 01), modeling reagent: lead acetate, digestion reagent: it is pure that red fuming nitric acid (RFNA) and perchloric acid are analysis; Plumbous standard reserving solution: 100mg/L (Jiangsu Prov. Disease Preventing and Controlling Center provides); Sampling Graphite Furnace Atomic Absorption spectrophotometer (AA-670 day island proper Tianjin).
2, animal used as test and grouping
The mouse of body weight 18-22g, the male and female dual-purpose, mouse conforms and raised for 1 week, equal ad lib standard feed, the grouping situation is as follows:
1. normal group: irritate stomach and give deionized water; 2. low dosage lead acetate model group: drink 0.5g/L lead acetate water solution, give normal feed; 3. high dose lead acetate model group: drink 1g/L lead acetate water solution, give normal feed; 4. positive controls: drink 1g/L lead acetate water solution gives calcium disodium edetate by 0.013ml/g bw; 5. carrot pectin group: drink 1g/L lead acetate water solution, press 8g/kg/d and take food the proportioning foodstuff of pectin by the free diet of mouse.
3. experimental technique
3.1 the preparation of animal model
To analyze pure acetate trihydrate lead [Pb (CH
3COO)
23H
2O] for being mixed with concentration with deionized water (or distilled water), the modeling material is respectively 1.0g/L, and the solution of 0.5g/L is pressed Pb
2+Count 546.2mgPb/L and 273.1mgPb/L.Making film and lead discharging carries out simultaneously.
3.2 medication
Calcium disodium edetate (CaNa
2EDTA) positive contrast medicine, every successive administration 3d drug withdrawal 2d. (irritated stomach and gave CaNa according to the dosed administration mosatil of 50mg/kg every day
2EDTA (13ml/kgbwd).
Carrot pectin is for being subjected to the reagent thing, with quantitative pectin with make the particle foodstuff, ad lib after normal foodstuff evenly mixes.Press 4g/kg.d, the administration respectively of 8g/kg.d dosage, administration 20 days.
Observe each cage mouse body weight, amount of drinking water calculates plumbous dosage, the indexs such as pectin food-intake taken in.
3.3 lead content is measured in the body
After last filling stomach was tried thing or deionized water 24h, eye socket was got blood, took off neck and put to death animal, got whole blood, liver and femur and carried out lead content mensuration.
The tissue digestion method: get liver, whole blood and femur and place the flask of crossing through acid soak respectively, (red fuming nitric acid (RFNA): perchloric acid=3: 1), WET DIGESTION is in sampling Graphite Furnace Atomic Absorption spectrophotometric determination lead content to add mixed acid.
4 experimental results
Experimental result shows, give lead acetate solution drinking-water continuously after, the mouse blood lead content obviously increases, low module reach high blood lead disease standard (100～199mg/L), high module be slight lead poisoning standard (200～249mg/L), show the modeling success.Wherein, the model group bone is plumbous increase the most remarkable, for the 45-55 of normal group doubly, illustrate that lead redistributes in vivo, and be deposited on bone.
The visible blood lead of pectin administration group, bone lead level obviously reduce, especially with high dose group and negative group comparing difference remarkable (p＜0.01).
But liver lead content model group and administration group difference are not obvious, and model group and administration group liver lead content are all lower.Liver lead is not seen obvious decline after the administration, is speculated as plumbously after the administration to circulate in enteron aisle through liver sausage and discharge, and mobilizes bone lead again to hepatic metastases and distribution, so the bone lead content obviously reduces, and liver lead still is in high-level state.Experimental result sees Table 1.
Table 1 carrot pectin is to the influence of lead content in the animal body
* compare p＜0.01 with high module comparison p＜0.05 * * and high module
Embodiment 6: carrot pectin row arsenic pharmacological evaluation
1, be subjected to the reagent thing:
Carrot pectin, prepare voluntarily (lot number: 04), modeling reagent oxidation arsenic, digestion reagent: it is pure that red fuming nitric acid (RFNA) and perchloric acid are analysis; Arsenic standard reserving solution: 100mg/L (Jiangsu Prov. Disease Preventing and Controlling Center provides); Sampling Graphite Furnace Atomic Absorption spectrophotometer (AA-670 day island proper Tianjin).
2, animal used as test and grouping
The mouse of body weight 18-22g, the male and female dual-purpose, mouse conforms and raised for 1 week, equal ad lib standard feed, the grouping situation is as follows:
1. normal group: irritate stomach and give deionized water; 2. low dosage arsenic oxide arsenoxide model group: drink 0.0012mg/L arsenic oxide arsenoxide solution, give normal feed; 3. high dose arsenic oxide arsenoxide model group: the drink 0.012mg/L arsenic oxide arsenoxide aqueous solution, give normal feed; 4. carrot pectin group: the drink 0.012mg/L arsenic oxide arsenoxide aqueous solution, press 4g/kg/d and 8g/kg/d and take food the proportioning foodstuff of pectin by the free diet of mouse.
3. experimental technique
3.1 the preparation of animal model
Be mixed with concentration with analysis pure zirconia arsenic modeling material with deionized water (or distilled water) and be respectively 0.0012mg/L, the solution of 0.012mg/L.
3.2 medication
Carrot pectin is for being subjected to the reagent thing, with quantitative pectin with make the particle foodstuff, ad lib after normal foodstuff evenly mixes.Press 4g/kg.d, the administration respectively of 8g/kg.d dosage, administration 20 days.
Observe each cage mouse body weight, amount of drinking water calculates arsenic and takes in dosage, indexs such as pectin food-intake.
3.3 arsenic content in the body
After last filling stomach was tried thing or deionized water 24h, eye socket was got blood, took off neck and put to death animal, got whole blood, liver and femur and carried out arsenic content.
The tissue digestion method: get whole blood and place the flask of crossing through acid soak, (red fuming nitric acid (RFNA): perchloric acid=3: 1), WET DIGESTION is in sampling Graphite Furnace Atomic Absorption spectrophotometric determination arsenic content to add mixed acid.
4. experimental result
Experimental result shows, give arsenic oxide arsenoxide solution drinking-water continuously after, mouse blood arsenic content obviously increases, low module reaches high blood arsenic disease standard, high module is slight arsenic poisoning standard, shows the modeling success.
The visible blood arsenic of pectin administration group level obviously reduces, especially with high dose group and negative group comparing difference remarkable (p＜0.01).
Experimental result such as table 2.
Table 2 carrot pectin is to the influence of blood arsenic content in the animal body
* and high module be p＜0.01 relatively
Embodiment 7: carrot pectin row mercury pharmacological evaluation
1, be subjected to the reagent thing:
Carrot pectin, prepare voluntarily (lot number: 02), modeling reagent mercury chloride, digestion reagent: it is pure that red fuming nitric acid (RFNA) and perchloric acid are analysis; Mercury standard reserving solution: 100mg/L (Jiangsu Prov. Disease Preventing and Controlling Center provides); Sampling Graphite Furnace Atomic Absorption spectrophotometer (AA-670 day island proper Tianjin).
2, animal used as test and grouping
The mouse of body weight 18-22g, the male and female dual-purpose, mouse conforms and raised for 1 week, equal ad lib standard feed, the grouping situation is as follows:
1. normal group: irritate stomach and give deionized water; 2. low dosage mercury chloride model group: the drink mercuric chloride solution, average dosage 1.5mg/kg gives normal feed; 3. high dose mercury chloride model group: the drink mercuric chloride solution, average dosage 2.5mg/kg gives normal feed; 4. carrot pectin group: drink 2.5mg/g mercuric chloride solution, press 4g/kg/d and 8g/kg/d and take food the proportioning foodstuff of pectin by the free diet of mouse.
3. experimental technique
3.1 the preparation of animal model
Be mixed with concentration with deionized water (or distilled water) and be respectively 1.5mg/kg to analyze pure mercury chloride modeling material, the solution of 2.5mg/kg is irritated stomach, modeling to mouse.
3.2 medication
Carrot pectin is for being subjected to the reagent thing, with quantitative pectin with make the particle foodstuff, ad lib after normal foodstuff evenly mixes.Press 4g/kg.d, the administration respectively of 8g/kg.d dosage, administration 20 days.
Observe each cage mouse body weight, amount of drinking water calculates mercury and takes in dosage, indexs such as pectin food-intake.
3.3 mercury content is measured in the body
After last filling stomach was tried thing or deionized water 24h, eye socket was got blood, took off neck and put to death animal, got whole blood and carried out mercury content mensuration.
The tissue digestion method: get whole blood and place the flask of crossing through acid soak, (red fuming nitric acid (RFNA): perchloric acid=3: 1), WET DIGESTION is in sampling Graphite Furnace Atomic Absorption spectrophotometric determination mercury chloride content to add mixed acid.
4. experimental result
Experimental result shows, give mercuric chloride solution drinking-water continuously after, mouse blood mercury content obviously increases, low module reaches high blood mercury disease standard, high module is slight mercury poisoning standard, shows the modeling success.
The visible blood mercury of pectin administration group level obviously reduces, especially with high dose group and negative group comparing difference remarkable (p＜0.01).
Concrete experimental result sees Table 3.
Table 3 carrot pectin is to the influence of blood mercury content in the animal body
* and high module be p＜0.01 relatively
Showing by above result of the test that carrot pectin has well combines as heavy metals such as lead, arsenic, mercury with heavy metal, the effect that heavy metal is excreted, and do not see bad reaction is arranged, therefore, carrot pectin be a kind of natural, row heavy metal effective, the product that has no side effect is expected to develop the health food or the medicine that become the row of a new generation heavy metal.
Claims (10)
1, a kind of carrot pectin, it is characterized in that, it is to make with following method: get the ammonium oxalate aqueous solution that dried carrot adds 3 to 15 times of amounts, at 30 to 100 ℃ of following constant temperature stirring reactions 0.5 to 3 hour, filtration, concentrate, add alcohol, Separation of Solid and Liquid, the taking precipitate of 1 to 6 times of amount, be drying to obtain carrot pectin.
2, the preparation method of the described carrot pectin of claim 1 is characterized in that may further comprise the steps:
Get the ammonium oxalate aqueous solution that dried carrot adds 3 to 15 times of amounts, in 30 to 100 ℃ of following constant temperature stirring reactions 0.5 to 3 hour, filtrations, concentrate, alcohol, Separation of Solid and Liquid, the taking precipitate of 1 to 6 times of amount of adding, be drying to obtain carrot pectin.
3. the method for preparing carrot pectin according to claim 2 is characterized in that, described carrot is the carrot pomace of carrot after extracting carrotene, and ammonium formiate concentration is 0.3% to 5%, and the consumption of ammonium oxalate is 15 times of the carrot quantity of slag.
4, according to claim 2 or the 3 described methods that prepare carrot pectin, it is characterized in that described reaction temperature is 90 ℃, the reaction time is 1.5 hours.
5, the method for preparing carrot pectin according to claim 4 is characterized in that, described alcohol is ethanol, and consumption of ethanol is 2 times of concentrate amount.
6, the described carrot pectin of claim 1 is used to arrange the application of heavy metal health food in preparation.
7, the application of carrot pectin according to claim 6 is characterized in that, carrot pectin is become jelly, jam or fruit juice with edible preparing carriers.
8, the application of carrot pectin according to claim 6 is characterized in that, carrot pectin is become food additives with edible preparing carriers.
9, the described carrot pectin of claim 1 is used to arrange the application of heavy metal medicine in preparation.
10, the application of carrot pectin according to claim 9 is characterized in that, carrot pectin and pharmaceutically acceptable carrier is made the medicine of tablet, capsule, granule, micro-capsule or ointment formulation.
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| CN200910029387A CN101524132A (en) | 2009-04-10 | 2009-04-10 | Carrot pectin and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910029387A CN101524132A (en) | 2009-04-10 | 2009-04-10 | Carrot pectin and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019005915A1 (en) * | 2017-06-27 | 2019-01-03 | Dupont Nutrition, Usa Inc. | Plant-derived colouring texturants |
-
2009
- 2009-04-10 CN CN200910029387A patent/CN101524132A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019005915A1 (en) * | 2017-06-27 | 2019-01-03 | Dupont Nutrition, Usa Inc. | Plant-derived colouring texturants |
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Application publication date: 20090909 |