CN101518261B - Rape drought-resistance seed-soaking agent and preparation method thereof - Google Patents
Rape drought-resistance seed-soaking agent and preparation method thereof Download PDFInfo
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- CN101518261B CN101518261B CN2009100614479A CN200910061447A CN101518261B CN 101518261 B CN101518261 B CN 101518261B CN 2009100614479 A CN2009100614479 A CN 2009100614479A CN 200910061447 A CN200910061447 A CN 200910061447A CN 101518261 B CN101518261 B CN 101518261B
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Abstract
The present invention discloses a rape drought-resistance seed-soaking agent and a preparation method thereof. The drought-resistance seed-soaking agent is prepared by mixing the following raw materials according to a certain proportion: GA3, KH2PO4 and 50 percent of mildothane. The method comprises the following steps: A, the GA3 and the 50 percent of mildothan are sufficiently stirred and evenly mixed through an agitator at normal temperature; and B, the drug combination prepared in the step (A) at the normal temperature is sufficiently stirred and evenly mixed with the KH2PO4 through the agitator to obtain an effective plant drought-resistant agent. The rape drought-resistance seed-soaking agent and the preparation method thereof provided by the present invention have the advantages of reasonable formula, convenient use, feasible method and simple and convenient operation. The innoxious, pollution-free and more ideal rape drought-resistance seed-soaking agent has low investment and high benefit, can remarkably improve the sprouting rate of the rape seeds, promotes the growth and development on-free and more ideal rape drought-resistance seed-soaking agent has low investment and high benefit, can remarkably improve the sprouting rate of the rape seeds, promotes the growth and development of the seedlings during the seedling stage, kills the pathogen of the seeds and finally achieves the purpose of resisting drought during the sprouting time of the rapes in the autumn of the seedlings during the seedling stage, kills the pathogen of the seeds and finally achieves the purpose of resisting drought during the sprouting time of the rapes in the autumn.
Description
Technical field
The present invention relates to plant growth regulator GA3 (gibberellin) and KH
2PO
4(potassium dihydrogen phosphate) plant regulator, more specifically relate to a kind of efficient rape drought-resistance seed-soaking agent, the preparation method who also relates to efficient rape drought-resistance seed-soaking agent simultaneously, it is applicable to resists area, middle and lower reach of Yangtze River rape seed phase arid, improves the rape germination rate and promotes the plumule growth.Relevant seed soaking agent has seed soaking agent that is applied to melon dish field and the seed soaking agent that is used to prevent and kill off bakanae disease of rice and strong sprout.And this product has been filled up area, raising middle and lower reach of Yangtze River rape germination period drought resistance, promotes the blank of the plant seed soaking agent invention in rape germination and plumule growth field.
Background technology
Crop is the restriction that is subjected to hereditary capacity and two kinds of factors of crop body physiological situation to the adaptation of adverse circumstance, and the latter has substantial connection with the crop hormone in vivo again.Utilize plant growth regulator to strengthen the resistivity of crop, become one of important channel of the degeneration-resistant cultivation of present crop adverse circumstance.
Area, the middle and lower reach of Yangtze River is as China rape main producing region, frequently take place based on the seasonal drought of autumn drought and winter drought, its in mid-autumn drought production has the greatest impact to rape.When autumn drought takes place generally on the occasion of the rape seed phase, normally season sowing of rape, and emerge slow, irregular, the basic seedling of emerging is few, usually is accompanied by the phenomenon that seed disease and mineral matter absorbing capacity descend, severe drought also causes sowing area to descend, and output is badly damaged.Need a kind of compound drought resistance agent of invention comprehensively to improve the drought resistance in rape germination period and seedling stage.
Use the drought that drought resistance agent is resisted to exist all the year round, for agrochemistry resisting drought saving water technology provides a brand-new approach.In recent years, the some kind drought resistance agents that occur on the market mostly are to improve the plant root water retention capacity or reduce transpiration by foliar spray in the mode of mixing soil, and compound drug effect of bound fraction and fertilizer efficiency reach the effect of control water strong sprout, combating drought and raising yields.And the seed soaking agent of minority also has been applied to improve the percentage of seedgermination of melon dish and paddy rice.But drought-resistance seed-soaking agent that also is not applicable to rape germination period arid at present and preparation method thereof is disclosed or uses.
Summary of the invention
The objective of the invention is to be to provide a kind of efficient rape drought-resistance seed-soaking agent, prescription rationally, and is easy to use, and the serious harm that production causes to china rape of reply autumn drought has reduced rape because the loss that the germination period arid causes.Plant growth regulator GA3 can breaking dormancy, increase content, the raising cell water absorbing capacity of the intracellular soluble substance of seedling, strengthen the activity of antioxidase system in the seedling cell, the ability that the raising tissue is resisted oxidation harm, can also activate carbohydrase and amylase system, regulation and control starch metabolism; KH
2PO
4Deng can regulate cellular signal transduction, keep osmotic equilibrium, the increased remineralization element.
Another object of the present invention is to provide a kind of method for preparing the high-efficiency plant drought resistance agent, compares with traditional drought resistance agent technology such as seed dressing and foliar spraies, and this product cost is low, and is easy to implement the method, easy and simple to handle.
Technical scheme of the present invention is to realize like this, a kind of efficient rape drought-resistance seed-soaking agent is characterized in that: by following raw material by mass percentage mixed preparing form: comprising GA3 (gibberellin, Wuhan timely rain bio tech ltd is purchased) 0.5-4%, KH
2PO
4(potassium dihydrogen phosphate, Wuhan timely rain bio tech ltd is purchased) 51-80%, 50% thiophanate methyl (Wuhan timely rain bio tech ltd is purchased) 16-45%.
Described drought-resistance seed-soaking agent is that mixed preparing forms by a certain percentage with a plant growth regulators, a kind of inorganic adjusting material and a kind of efficient germicide etc.Add water to 200ml after during use this product being dissolved in a small amount of alcohol or liquor, seed is 1/1 with dilution back seed-soaking liquid mass volume ratio (W/V).Seed soaking time is 6-8 hour.After the seed soaking the clean back of drying of seed is sowed at once.
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 0.5-4％
KH
2PO
4 51-80％
50% thiophanate methyl 16-45%.
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 2.9-4％
KH
2PO
4 51-58.5％
50% thiophanate methyl 36.8-45%
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 2.2-3.4％
KH
2PO
4 58.1-66.8％
50% thiophanate methyl 28.2-38.5%.
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 1.4-2.7％
KH
2PO
4 66.2-73.4％
50% thiophanate methyl 22.4-31.6%.
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 0.5-1.6％
KH
2PO
4 73.6-80％
50% thiophanate methyl 16-25.5%.
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 1.3％
KH
2PO
4 76％
50% thiophanate methyl 22.7%.
A kind of method for preparing the high-efficiency plant drought resistance agent the steps include:
A, under normal temperature (4 ℃ or 8 ℃ or 15 ℃ or 23 ℃ or 28 ℃), the GA3 of 0.5-4% and 50% thiophanate methyl of 16-45% are fully mixed with agitator, just obtain a kind of mixture of medicament.
B, the confection that under normal temperature (4 ℃ or 8 ℃ or 15 ℃ or 9 ℃ or 23 ℃ or 28 ℃), makes in step (A) and the KH of 51-80%
2PO
4Fully mix with agitator, just obtain a kind of high-efficiency plant drought resistance agent.
C, three kinds of well-beaten confections of raw material are packed as the inner wrapping of 2 ± 0.2 gram/bags.Each bag can be used for one mu of rape seed soaking.
Advantage and application prospect:
This product adopts that drought resisting is remarkably productive, application technology is ripe, the more deep plant growth regulator GA3 of research is a core, the CaCl that is easy to get with raw material
2, KH
2PO
4Etc. inorganic adjusting material is composite dose.This product have drop into low, high efficiency, characteristics such as operating technology is simple are a kind of nontoxic, nuisanceless, comparatively ideal rape drought-resistance seed-soaking agents.This product can significantly improve the rape seed germination rate, promotes seedling growth to grow, and kills the seed cause of disease, finally reaches the purpose of resisting rape germination period in autumn arid.Its cost is low, and effect is remarkable, and seed soaking technology simple and feasible, and the new way that rape drought-resistance increases production has been opened up in strong adaptability, has broad application prospects on producing.
The present invention can be effectively ploughs direct-sowing rape with few (exempting from) during China rape is produced and gently simplifies the cultivation new model and combine; reduced production cost; reduced labour's input; brought into play the potential of plant growth regulator fully; simultaneously; rape production mechanization in the future also will be widely used in the process of scale, bring into play more important role in resisting the drought harm of rape autumn.
Area, the middle and lower reach of Yangtze River is China winter rape main producing region, and the rape output reaches 85% of the whole nation.And because the regional gross output underproduction that the autumn drought causes can reach 25%-32%.Increasing the weight of of the autumn drought harm that causes along with the continuous expansion and the climatic variation of rape seed area, the present invention will have very huge market application foreground.Use this product, can significantly reduce the loss of the rape production that brings because of autumn drought harm, create huge economic and social benefit.
Embodiment
Embodiment 1:
The efficient rape drought-resistance seed-soaking agent of a kind of rape seed soaking, it by following raw material by mass percentage mixed preparing form:
The raw material mass percent
GA3 1.3％
KH
2PO
4 76％
50% thiophanate methyl 22.7%.
A kind of method for preparing the high-efficiency plant drought resistance agent the steps include:
A, under normal temperature (4 ℃ or 8 ℃ or 15 ℃ or 9 ℃ or 23 ℃ or 28 ℃), the GA3 of 0.5-4% and 50% thiophanate methyl of 16-45% are fully mixed with agitator, just a kind of mixture of medicament.
B, the confection and the 51-80%KH that under normal temperature (4 ℃ or 8 ℃ or 15 ℃ or 9 ℃ or 23 ℃ or 28 ℃), make in step (A)
2PO
4Fully mix with agitator, just obtain a kind of high-efficiency plant drought resistance agent.
C, three kinds of well-beaten confections of raw material are packed as the inner wrapping of 2 ± 0.2 gram/bags.Each bag can be used for one mu of rape seed soaking.
A kind of efficient rape drought-resistance seed-soaking agent, it is made by the raw material of following mass percent:
| Embodiment | GA(％) | KH 2PO 4(％) | 50% thiophanate methyl (%) |
| Embodiment 2 | 3.2 | 59 | 37.8 |
| Embodiment 3 | 2.2 | 65 | 32.8 |
| Embodiment 4 | 1.7 | 68 | 30.3 |
| Embodiment 5 | 3.6 | 53 | 43.4 |
| Embodiment 6 | 2.7 | 66.5 | 30.8 |
| Embodiment 7 | 1.4 | 72 | 26.6 |
| Embodiment 8 | 1.5 | 70.5 | 28 |
| Embodiment 9 | 3.9 | 58 | 38.1 |
| Embodiment 10 | 3.1 | 66.8 | 30.1 |
| Embodiment 11 | 2.1 | 70 | 27.9 |
| Embodiment 12 | 1.1 | 79.4 | 19.5 |
| Embodiment 13 | 0.6 | 76.4 | 23 |
| Embodiment 14 | 0.8 | 76.9 | 22.3 |
| Embodiment 15 | 2.4 | 62.6 | 35 |
| Embodiment 16 | 2.9 | 57.7 | 39.4 |
Its preparation process is identical with embodiment 1.
With in Inst. of Oil Crops, Chinese Academy of Agriculture's seed selection two No. 10 be experiment material.Choose size evenly, full rape seed is soaked seed with this product, is contrast with the clear water, adds water to 200ml after during use inner wrapping this product being dissolved in a small amount of alcohol or liquor, and seed is 1/1 with dilution back seed-soaking liquid mass volume ratio (W/V).Seed soaking time is 6-8 hour.After the seed soaking the clean back of drying of seed is sowed at once.
Seeds treated is carried out germination test, and germination test is with reference to standard GB/T3543.4-1995, with 15%PEG-6000 solutions simulate rape germinate and seedling stage drought condition.Sow and observe germination rate after 7 days, every processing picked at random 10 strain seedling are measured height of seedling, and fresh weight also calculates its relative vitality index: relative vitality index=relative height of seedling * relative germination rate, test repeats 3 times.
Simultaneously, get the mensuration that seedling cotyledon carries out the Physiology and biochemistry field:
1) mensuration of electrical conductivity: measure with DDS-11A type conductivity gauge.
2) mensuration of content of soluble protein is pressed Coomassie brilliant blue method mensuration.
3) the active guaiacol colorimetric method for determining of using of peroxidase (POD).
4) the cat catalase activity is built up the CAT kit measurement that bio-engineering corporation produces with Nanjing.
5) malonaldehyde (MDA) content adopts thiobarbituricacid (TBA) determination of color.
6) proline content is measured and is adopted ninhydrin.
The result:
| Index | Rate of change % | Index | Rate of change % | Index | Rate of change % |
| Germination rate | +12.6～15.3 | Soluble protein | +26.4～35.6 | CAT | +21.8～45.6 |
| Height of seedling | +33.7～72.7 | Free proline | +55.1～62.9 | POD | +23.1～46.6 |
| Vitality index | +40.1～68.8 | Electricity leaks | -26.2～36.5 | MDA | -21.9～36.6 |
The result shows, the seed soaking of this product make rape germination and seedling stage drought-resistance ability significantly improve.This seed soaking agent can be by keeping water balance as a kind of conditioning agent at rape seedling, reduce oxidative stress, breaking dormancy, kill the seed cause of disease, promote starch metabolism to help resist drought stress, and reach and promote rape to germinate and the effect of seedling growth, can be used as a kind of resist the rape germination and seedling stage drought stress the efficient drought-resistant conditioning agent apply.
Claims (4)
1. rape drought-resistance seed-soaking agent, it is characterized in that: it is made by mass percentage by following raw material:
2. a kind of rape drought-resistance seed-soaking agent according to claim 1 is characterized in that: it is made by mass percentage by following raw material:
3. a kind of rape drought-resistance seed-soaking agent according to claim 1 is characterized in that: it is made by mass percentage by following raw material:
4. method for preparing the described rape drought-resistance seed-soaking agent of claim 1, it may further comprise the steps:
A, at normal temperatures the GA3 of 0.5-4% and 50% thiophanate methyl of 16-45% are fully mixed with agitator, just obtain the mixture of medicament;
B, at normal temperatures with the confection that makes in the step (A) and the KH of 51-80%
2PO
4Fully mix with agitator, make to obtain a kind of high-efficiency plant drought resistance agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100614479A CN101518261B (en) | 2009-04-07 | 2009-04-07 | Rape drought-resistance seed-soaking agent and preparation method thereof |
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|---|---|---|---|
| CN2009100614479A CN101518261B (en) | 2009-04-07 | 2009-04-07 | Rape drought-resistance seed-soaking agent and preparation method thereof |
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| CN101518261A CN101518261A (en) | 2009-09-02 |
| CN101518261B true CN101518261B (en) | 2011-09-14 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817708B (en) * | 2010-04-29 | 2012-11-21 | 司文会 | Plant source seed soaking agent and preparation method thereof |
| CN102786347A (en) * | 2012-08-15 | 2012-11-21 | 中国农业科学院油料作物研究所 | Rape growth regulator and preparation method thereof |
| CN103283784A (en) * | 2013-06-24 | 2013-09-11 | 合肥多加农业科技有限公司 | Long-acting environment-friendly paddy-rice seed-soaking agent and application method thereof |
| CN104885631A (en) * | 2014-10-17 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | Germinating method for rape seeds |
| CN104641761B (en) * | 2015-01-30 | 2016-05-04 | 巢湖市鑫宇良种农民专业合作社 | Rape seed sowing pre-treating method |
| CN105503393A (en) * | 2015-12-18 | 2016-04-20 | 中国农业科学院油料作物研究所 | Rape seed soaking agent and preparation method and application |
| CN105941405B (en) * | 2016-07-15 | 2018-11-20 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape drought stress |
| CN110402962B (en) * | 2019-08-23 | 2021-11-16 | 湖北省农业科学院中药材研究所 | Plant growth regulating composition and application thereof |
| CN114557168B (en) * | 2022-01-19 | 2023-03-17 | 中国建筑股份有限公司 | Composite seed capsule for rapidly forming plant community and preparation method thereof |
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| CN1580010A (en) * | 2004-05-19 | 2005-02-16 | 王树禹 | Dry farm cultivating agent |
| CN1820608A (en) * | 2006-01-13 | 2006-08-23 | 如东县作物栽培指导站 | Anti-disaster agent for disaster-stricken cotton remedy and producing method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580010A (en) * | 2004-05-19 | 2005-02-16 | 王树禹 | Dry farm cultivating agent |
| CN1820608A (en) * | 2006-01-13 | 2006-08-23 | 如东县作物栽培指导站 | Anti-disaster agent for disaster-stricken cotton remedy and producing method |
Non-Patent Citations (1)
| Title |
|---|
| 陆子梅,等.赤霉素和营养物质对油菜种子萌发及秧苗生育的协同作用.《天津农业科学》.2002,第8卷(第2期),第15-17页. * |
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